148
TIRUPATI.
SAMPLES TAKEN—3RD.SEPTEMBER 1909.
REMARKS.—The Kalyani river is partially flooded. About 13 inches of rain have fallen since last analysis. The supply is constant.
Bacteriological Analyses.
SAMPLE I.—A SHALLOW WELL ON THE BANK OF K.ALTANI
RIVER TO THE EAST OF HEAD WORKS (UNFILTERED WATER) . C o l o n y n u m b e r .
L a c t o s e .
S a c c h a r o s e .
D u l c i t e . A d o n i t e .
I n u l i n .
V o g e s a n d P r o s k .
I n d o l .
M o t i l i t y .
G r o u p .
Bacillus.
G r o u p a n d R e m a r k s .
(a) Total colonies per cc. on agar at 37°—1,960.
(b) Bile salt lactose, medium.
+
—
1 tube 20 cc. of water taken
. . 1
..
2 tubes 10 cc.
,,
.. 2
..
1 + + + + – + - - III 67 ..
..
.. Group I,n i l ,
3 ,, 5 cc.
,, .. 3
..
2 + - + - - - + - II Schafferi . . ,, II, 4.
3
, ,
1 c c .
. . 3
..
3 + - + - - - + - II Do.
..
..
, , I I I , 3 .
3 ,, .1 cc.
, ,
3
..
4 + + - + - + - - IV Lactis ærogenes. . . ,, IV, 3.
3 „
. 0 1 c c .
, ,
. . ..
3
5 + + - + - + - - IV
Do.
..
6 + + - + - - - - IV Gasoformans
..
7 + + + + - + - - III 67 ..
..
.. Class 1, 4,
8 + + + + - + - - III 67 ..
..
. . ,, II, 6.
9 + - + - - - + +
I I Coli communis.
. .
III,n i l ,
(c) Fœcal bacilli in .1 cc. and upwards.
10 + - + - - + + II
Do.
.. Undetermined.
(d) Glucose fermenters in 01 cc. and upwards.
nil.
SAMPLE II.—A SHALLOW WELL ON THE BANK OF KALYANI
RIVER TO THE WEST OF HEAD WORKS AT A DISTANCE OF
ABOUT 320 FEET FROM THE FIRST(UNFILTERED WATER).
1 + + - - - + - + IV Cloacæ
..
.. Group I, ni l ,
2 + + - - - + - + IV Do.
..
. . ,, II, ni l .
3 + + - - - + - + IV Do.
..
. . ,, III, ni l .
4 + + - - - - - + IV 109 ..
..
. . ,, IV, 1 0 .
(a) Total colonies per cc. on agar at 37º—6,300.
5 + + - - - - - + IV 109 ..
..
..
6 + + - - - + - + IV Cloacæ
..
..
(b) Bile salt lactose, medium
+
-
7 + + - - - + - + IV
Do.
..
.. Class I, n i l .
8 + + - - - + - + IV Do.
..
. .
, , I I , 1.
9 +
+ - + - + - - IV Lactis ærogenes ..
, , I I I , 9 .
1 tube 20 cc. of water taken
..
1
.. 10 ++ - - - + - + IV Cloacæ
..
.. Un d e t e r mi n e d .
2 tubes 10 cc.
, ,
. .
2
. .
nil.
3 „ 5 cc , ,
. . 3
. .
3 „ 1 cc.
,,
.. 1
2
3 „
. 1 c c .
, ,
. . 1
2
3 „
. 0 1 c c .
, ,
. . ..
3
(c) Fœcal bacilli in 5 cc. and upwards.
(d) Glucose fermenters in .1 cc. and upwards.
SAMPLE III.—COLLECTING CHAMBER—FILTERED WATER.
1 + + - + - + - +
I V 102 ..
..
Group I, 1,
2 + - + - - - + + II Coli communis
.. ,, II, 1,
(a) Total colonies per cc. on agar at 37°—1 colony
occupying whole plate.
3 + + - + - + - + IV 102 ..
..
.. „ III, n i l
4 + + - + - + - - IV Lactis ærogenes .. „ IV, 8.
5 + + - + - + - + IV 102 ..
..
(b) Bile salt lactose, medium.
+
- 6 + - - - - - + I 7
..
..
..
7 + + - + - + - + IV 102 ..
..
.. Class I, 1.
8 + + - + - + - + IV 102
..
. .
II, 8.
1 tube 20 cc. of water taken
. .
1
. . 9 + + - + - + - + IV 102
. .
. .
..
, , I I I , 1
2 tubes 10 cc.
, ,
. .
2
. . 10 + + - + - + - + IV 102 ..
..
.. Undetermined.
3 „ 5 cc.
, ,
. .
3
. .
ni l .
3 „ 1 cc.
, ,
. . 2
1
3 „
. 1 c c
, ,
. . . .
3
3 „
. 0 1 c c
, ,
. . ..
3
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Glucose fermenters in 01 cc. and upwards.
SAMPLE IV.—FROM A TAP IN WESTERN CORNER, 7½ MILES
FROM HEAD WORKS. A FEW YARDS OFF THE MAHUNT'S
HIGH SCHOOL.
1 + + - + - + - + IV 102 ..
..
.. Group I,1.
2 + - + - - - - + II 36 ..
..
.. ,, II, 2.
3 + + - + - + - + IV 102 ..
..
.. „ III, 1.
4 + + - + - + - - IV Lactis ærogenes
. . „ IV,7.
(a) Total colonies per cc. on agar at 37°—8,190
5 + +
- - - - + - IV Coscoroba ..
. .
6 + - + - - - + + II Coli communis.
. .
(b) Bile salt lactose, medium.
+
- 7 + + - + - - - + IV
. . .
Class 1, 2.
8 + - - + - - + + I
1
. .
. .
. .
, , I I , 6
9 + +
+ + - + + - III 76 ..
..
..
, , I I I ,
n i l .
1 tube 20 cc. of water taken
. . 1
. . 10 + + - + - - + - IV 101 .. ..
. . Undetermined.
2 tubes 10 cc
, ,
. .
2
. .
n i l .
3 „ 5 cc.
,,
. .
3
. .
3 „ 1 cc.
, ,
. .
3
. .
3 „ . 1 c c .
, ,
. .
3
. .
3 „
. 0 1 c c .
„ . . ..
3
(c) Fœcal bacilli in .1 cc. and upwards.
(d) Glucose fermenters in .01 cc.