P. R KRISHNA IYER 103
presumably by the alimentary route. Most of
the diseased animals were in fairly good condi-
tion, and in some the condition was excellent.
Calcification of the lesions (15 out of 25 cases)
was not uncommon. Of the six cases at Izat-
nagar, three alone yielded tubercle bacilli in
culture. Whilst 16 cases were detected at
Bombay and confirmed microscopically, mate-
rial from only eight cases was inoculated into
guineapigs. Of the three cases in Calcutta the
organism was recovered from two: thus 13
strains were available for typing.
TYPING OF TUBERCLE BACILLI FROM BUFFALOES
Cultural. Tubes
of plain egg (Dorset
medium) inoculated from the precural and sub-
lumbar glands and spleen of affected guinea
pigs were incubated at 37°C. Growth appeared
after five weeks or in some cases after nine
weeks. The average number of days for appear-
ance of growth in primary culture was 53 days.
The second and third generation cultures grew
more quickly but growth was not prolific. Cul-
turally, the buffalo tubercle strains appeared to
be typically dysgonic and were nearly always
restrained by added glycerin, with one excep-
tion (strain 7), which grew equally in plain and
in glycerinated egg media.
Animal inoculation. Guinea-pig inoculation
with organ-suspension from the primary lesion
appeared to indicate that the virulence of the
organism was lower than that usual for bovine
types, since in some cases five months elapsed
before death. Experiments with the second and
third generation cultures indicated, however,
that the virulence was of standard type. Table
II. shows the results of virulence tests in rabbits,
guinea-pigs and fowls:
TABLE II
Strain
1
2
3
4
Animal
G. pig .
„ .
„ .
„ .
Rabbit .
„ .
„ .
Fowl
„ .
G. pig
„ .
„ .
„ .
Rabbit
„ .
„ .
Fowl
„ .
G. pig
„ .
„ .
Rabbit .
„ .
Fowl
„ .
Rabbit
„ .
„ .
„ .
„ .
G. pig .
„ .
Inoculum
Gland suspension 0„5 c.c. S/c .
„ „ „ .
1.0 mg. of 2nd gon. cult. S/c. .
0.1 mg.
„
„
„ .
0.1 mg.
„
„
I/v. .
„ „ „ . . .
5.0 mg.
„
„
S/c .
1.0 mg.
„
„
I/v..
0.01 mg.
„
„
„ .
0.5 c.c. of gland suspension S/c
„ „ „ .
1.0 mg. 2nd gen. cult. S/c .
0.1 „ „ „ .
0.1 mg.
„
„
I/v. .
0.01 mg. „ „ „ .
5.0 mg.
„
„
„ .
1.0 mg.
„
„ „ .
0.01 mg. „
„
„ .
0.5 c.c. gld. suspension S/c .
„ „ „ .
1.0 mg. 2nd gen. cult. S/c .
0.1 mg. „ „ .
0.1 mg.
„
I/v .
0.01
mg.
„ „ .
5.0 mg.
„
S/c. .
1.0 mg.
„
I/v .
0.01 mg. „ „ .
5.0 mg. 2nd gen. cult. S/c .
0.1 mg.
„
„ I/v .
0.01 mg. „
„ „ .
0.1 mg. 4th gen. „
„
0.01 mg. „ „ „ .
1.0 mg. 2nd gen. „
S/c .
0.1 mg.
„
„
„ .
Result
(days)
K. 105 .
K. 66 .
D. 60 .
D. 67 .
D. 22 .
D. 38 .
D. 101 .
D. 54 .
D. 190 .
K. 64 .
K. 99 .
D. 54 .
D. 91 .
D. 52 .
D. 38 .
D. 114
.
K. 248
.
D. 19 .
K. 94 .
K. 59 .
D. 24 .
D. 66 .
D. 25 .
D. 29 .
D. 50 .
D. 32 .
K. 248
.
D. 58 .
D. 15 .
D. 19 .
D. 30 .
D. 33 .
D. 42 .
D. 50 .
Post mortem
G. T.
G. T.
G. T.
G. T.
G. T.
G. T.
G. T.
Spirochaetosis. No T. B.
Death due to other causes. No T. B.
Enlarged and caseous local and lumbar gld.
„ „ „ „
G. T.
G. T.
G. T.
G. T.
G. T.
No T. B.
No T. B.
G. T.
G. T.
Peritonitis
G. T.
G. T.
G. T.
G. T.
Ranikhet disease
No T. B.
G. T.
T. B. ?
T. B. ?
G. T.
G. T.
G. T.
G. T.
S/c = Subcutaneously.
I/v = Intravenously
D. = Died
K. = Killed
G. T. = Generalized tuberculosis