J.
CARMICHAEL
189
composed of epitheloid and lymphocytic cells
whilst there are also occasional concentric fibrosed
foci involving a few alveoli.
(3) ZEBU CALF NO. 3275
Received 50 mg. of strain B. V. 28 on
May 31, 1938. Killed May 20 1939. Duration
of infection: 354 days. Weight at commence-
ment 185 lb. Weight at conclusion: 380 lb.
Post-mortem. Excellent condition and appears
in perfect health. The only abnormality seen
was the regional gland (right prescapular), which
was about twice the normal size—hæmorrhagic on
section and showing an encapsulated tubercu-
-ous abscess about 1 c.m. in diameter and contain-
ing fibro-caseous material showing early calcifi-
cation. Lungs, spleen and all other glands
normal.
This animal had obviously recovered from the
tuberculous infection and shows almost complete
resistance.
It showed intermittent pyrexia for the first
three weeks but gained steadily in weight.
Sections. Lung: Lung normal except for very
occasional minute circumscribed fibrosed areas
involving only one or two alveoli. There are also
very occasional areas of circumsrcibed consolida-
tion consisting of a mass of lymphocytes rather
resembling a lymph node. These usually occur
near a small bronchiole and occupy three or
four alveoli only.
Controls. (1) Rabbit No. 1071: Received 10
mg. of B. V. 28 on May 31, 1938, subcutaneously
and died after 92 days of severe generalized
tuberculosis. Post-mortem:
Local abscess.
Tuberculosis of lungs and spleen and all glands
and raised tubercles on kidneys.
(2) Rabbit No. 1072 : Received 10 mg. of
B.V. 28 on May 31, 1938, subcutaneously and
died after 99 days of severe generalized tuber-
culosis. Post-mortem: Local abscess. Tuber-
culosis of lungs and spleen and all glands. Raised
tubercles on kidneys.
Experiment No. 3
ZEBU CALF NO. 3274
Received 50 mg. of strain B.V. 28 on August
27, 1938. Killed May 20, 1939. Duration of
infection: 266 days. Weight at commencement:
140 lbs. Weight at conclusion : 168 lbs.
Post-mortem. Fair condition. Regional gland
(right prescapular) slightly enlarged and firm
and showing a few discrete encapsulated tubercles
about the size of pea and tending to calcify.
A subcutaneous tuberculous abscess in this region
had already drained. Caseo-fibrous lesions show-
ing early calcification were seen in the left pres-
capular, presternal, mediastinal, axillary (right)
hepatic and left precrural glands.
Spleen showed numerous firm encapsulated
tubercles 3.8 mm. diameter throughout the
organ. Lungs showed many translucent firm
miliary tubercles throughout.
There were
numerous tubercles 3-4 mm. in diameter scattered
over the omentum.
The liver showed numerous firm small miliary
tubercles below the capsule and throughout the
organ. All showed early calcification.
This
animal had not resisted the infection as well as
others but it had gained in weight and the tendency
was to complete clinical recovery.
Sections. Lung: This section shows rather
more extensive and diffuse areas of consolidation
consisting of masses of epitheloid cells in which
there is evidence of early fibrosis. There is no
giant cell formation. There are several circum-
scribed and fairly large tubercle-like formations
consisting of masses of epitheloid cells through
which there are well-marked concentric stands of
fibrous tissue and in the centre an irregular area
of calcification. Other areas are very tiny
circumscribed areas of scar tissue involving
only two or three alveoli with or without a tiny
centre of calcified material.
Controls. Goat No. 1106 and Goat No. 1107
inoculated with 10 mg. of same strain subcuta-
neously. Both died 39 days later and on post-
mortem showed severe miliary tuberculosis of
the lungs with a local lesion in the regional gland—
right prescapular.
Experiment No. 4
ZEBU CALF NO. 3272
Weight 152 lb. Inoculated subcutaneously
with 50 mg. of a six-day-old culture B.V. 34
on December 22, 1939. Killed 91 days later.
Post-mortem. Weight 175 lb., an increase of
23 lb. Excellent condition. Right prescapular
gland (regional gland) enlarged, indurated, and
full of dry caseo-fibrous material. All other
glands normal. Lungs showed a very very
scattered miliary tubercles with no perituber-
cular reaction.
Sections. Lung: A section of a normal lung
with very rare tiny areas of concentrically ar-
ranged scar tissue involving one or two alveoli
and having in the centre a dot of calcified material
or a small cavity, or they may be entirely solid.
Liver: Normal except for rare and very
small and irregular-shaped localized infiltrations
of darkly stained stellate cells. Regeneration of
liver tissue appeared to be complete.
Controls. (1) Goat No. 987: Inoculated sub-
cutaneously with 10 mg. of the same culture.
Died after 30 days of severe generalized tuber
culosis.
(2) Rabbit No. 986: Inoculated subcuta-
neously with 10 mg. of the same culture. Died
after 87 days of severe generalized tuberculosis.