188
Bovine Tuberculosis
tuberculosis. Local abscess. Lungs, spleens,
kidney and all glands tuberculous.
ZEBU CALF NO. 3281.
Killed after a period of 90 days. Condition
fair. Weight same as at time of inoculation, i. e.,
129 lbs. Regional gland (right prescapular) enlarg-
ed and indurated and on section contained firm,
dry, caseous material tending to calcify. A
soft purulent abscess in adjacent subcutaneous
tissues. Presternal gland enlarged and indurated
and on section showed fibro-caseous material
tending to calcify. Liver showed numerous
very tiny miliary tubercles below the capsule.
Hepatic gland slightly enlarged. Lungs normal
in consistency but containing numerous very
small translucent miliary tubercles.
A guinea pig inoculated with 0.5 c.c. of an
emulsion from the prescapular gland died of severe
generalized tuberculosis 106 days later.
Sections. Lung: Interalveolar septa generally
thickened and congested with occasional circum-
scribed consolidations composed of fibroblasts
and lymphocytic cells disposed in a matrix of
structureless hyaline-like material with or with
out a calcified centre. No giant cells present.
Liver. Very small tubercles—few in number.
Epitheloid cells almost entirely replaced by a
ring of lymphocytes. Centre of tubercles shows
destruction of liver and in some cases complete
necrosis, whilst in other cases there was an
infiltration with lymphocytes and epitheloid cells.
Giant cells frequent.
ZEBU CALF NO. 2562.
Killed after a period of 90 days. Increase
in weight 34 lb. Condition fairly good. Regional
gland (right prescapular) consisted of a large
caseo-fibrous tumour-like mass. Presternal gland
enlarged and gland substance entirely replaced
by dry caseo-fibrous material tending to calcifica-
tion. Spleen showed a few miliary tubercles
scattered throughout the pulp. Liver showed
numerous miliary tubercles scattered throughout
the organ. Lungs showed a few discrete translu-
cent miliary tubercles.
The impression obtained from the post-mortem
examination of these two calves was that the
general tendency was one of resolution and
healing. There was a distinct tendency to cal-
cification in the gross lesions in the glands whilst
the miliary tubercles in the parenchyma of the
organs were discrete and firm in consistency,
giving the impression of sequestration and
resolution.
The pulmonary tubercles in calf No. 3281,
described as translucent, appeared to be under,
going a hyaline change.
Experiment No. 2
(1) ZEBU CALF NO. 3279
Received 50 mg. subcutaneously of strain
B.V. 28 on May 31, 1938. Killed May 20, 1939.
Duration of infection: 354 days. Weight at
commencement: 132 lb. Weight at conclusion:
284 lb.
Post-mortem. Condition
good.
Regional
gland (right prescapular) about twice normal
size and surrounded by a thick fibrous capsule.
A tuberculous abscess about the size of a walnut
well encapsulated and containing dry caseo-
fibrous material is seen at the upper pole of the
gland. The rest of the gland is normal with the
exception of a small encapsulated abscess about
1 c.m. diameter containing dry calcified tuber-
culous material.
No lesions in the lungs
and rest of body normal. This animal has
completely recovered from the tuberculous in-
fection and was in perfect health.
One guinea pig injected with 0.5 c.c. of emulsi-
fied prescapular gland died of generalized tuber-
culosis after 189 days.
Sections. Lung: The section has all the
characteristics of a normal lung. There appears
to be a slight thickening of some of the interal-
veolar septa, and there are very occasional tiny
circumscribed fibrosed areas involving perhaps
six or seven alveoli. The fibrous tissue is laid
down in a concentric manner and in the centre
there may be a microscopic cavity or a small
dot of calcified material.
(2) ZEBU CALF NO. 3277
Received 50 mg. of strain B.V. 28 on May 31,
1938. Killed January 14, 1939. Duration of
infection: 228 days. Weight at commencement:
153 lb. Weight at conclusion: 243 lb.
Post-mortem. Excellent condition and shows
every sign of health. Regional gland (right
prescapular) about three times normal size and
full of creamy yellow pus forming a large tuber-
culous abscess. Lungs showed a few scattered
millet seed-like tubercles—hard and obviously
healed.
Spleen normal in size and showed old millet
seed hard tubercles showing early calcification.
Middle cervical gland firmly encapsulated and
containing caseo-calcareous matter.
Mediastinal gland slightly enlarged and indurat-
ed . 0.5 c.c. of caseous matter from prescapular
gland emulsified and injected subcutaneously
into two guinea pigs which died of acute genera-
lized tuberculosis 124 days later.
This animal showed intermittent pyrexia for
the first month but gained steadily in weight.
Section. Lung: Most of the section shows
normal lung tissue. There are occasional areas
where the alveoli are filled with a cellular exudate