93
TIRUPATI.
SAMPLES TAKEN—13TH JUNE 1909.
REMARKS.—There has been 11.3 inches of rain since last report.
Bacteriological Analyses.
SAMPLE I.—A SHALLOW WELL ON THE BANK OF KALYANI
RIVER TO THE EAST OF HEADWORKS—UNFILTERED WATER..
Co l o n y n u mb e r .
S a c c h a r o s e .
Dul ci t e.
Adoni t . I nul i n.
V o g e s o r P r o s k .
I ndol .
M o t i l i t y .
Group.
Bacillus.
Groups and
Remarks
.
(a) Total colonies on agar at 37° — countless.
(b ) Bile salt lactose, medium.
+
—
1 tube 20 cc. of water taken ...
1
...
1 + — — — + + + IV
......
Group I, n i l .
2 tubes 10 cc.
„
2
. . . 2 + — + — + + — IV
......
„ II, nil.
4
„
5 cc
.
„
...
...
4
... 3 +
+
+ — — + — III
......
„ III, 7.
5 „ 1 cc. „
"
5
. . . 4 + + + — + + + III
. . . . . .
„
IV,
3.
5 „ .1 cc.
„
,,
...
...
5
. . . 5 + + + — + + — III
5 „ .01 cc.
„
,,
...
....
1
4
6 + + + — + + — III
7 + + + — + + +
I I I
(c) Fœcal bacilli in .1 cc. and upwards.
8 + + + — + + — III
9 + — — — + + +
I V
(d) Sporogenes milk test—in 20 cc.
10 + + + — + + — III
SAMPLE II.—A. SHALLOW WELL ON THE BANK OF KALYANI
RIVER TO THE WEST OF HEADWORKS AT A DISTANCE OF 32
0 FEET FROM THE FIRST—UNFILTERED WATER.
1 + — + — + — — IV B. Lactis ærogenes... Group I, nil.
2 + — + — + — — IV
Do.
„
II, nil.
3 + — + — + — — IV
Do. „.
„ III, 4.
4 + + + — + — — III B. No. 67
„
IV, 6.
(a) Total colonies on agar at 37° — countless.
5 + + + — + — — III B. No. 67.
+
—
6 + — + —+ — + IV B. No. 102.
(b) Bile salt lactose, medium.
7 + — + — + — + IV B. No. 102.
8 + — + — + — + IV B. No. 102.
1 tube 20 cc. of water taken ...
...
1
..
9 + + + — + — +
I I B. No. 73.
2 tubes 10 cc.
„
...
...
2
...
10 + + — — + — + III
4 „ 5 cc.
„
...
...
4
5 „ 1 cc.
„
...
...
5
...
5 „ .1 cc.
„
7,
...
...
5
...
5 „ .01 cc.
„
...
...
5
...
(c) Fœcal bacilli in .01 cc. and upwards.
(d) Sporogenes milk test —in 20 cc.
SAMPLE III.—COLLECTING CHAMBER—FILTERED WATER..
1 + — + — + — — IV B. Lactis ærogenes ... Group I, nil.
2 + — + — + — — IV
Do.
...
„ II, nil.
(a) Total colonies on agar at 37° —countless.
3 + — + — + — — IV
Do.
...
„ III, 1.
+
—
4 + — — —+ — — IV
......
„ IV, 9.
(b) Bile salt lactose, medium.
5 + — + — + + — lV
6 + — + ——+ + IV
1 tube 20 cc. of water taken
..,
..,
1
...
7 + — + — + — — IV B. Lactis ærogenes.
2 tubes 10 cc.
„
2
...
8 + — + — + — — IV
Do.
4 „ 5 cc.
„
4
...
9 + — + — + — — IV
Do.
5
„ 1 c c . „
5
...
10 + + + — + — — III
5 „ .1 cc.
„
5
...
5
„
.
01 cc.
„
5
. .
(c) Fœcal bacilli in .01 cc. and upwards.
(d) Sporogenes milk test—in 20 cc.
SAMPLE IV.—FROM A TAP IN WESTERN CORNER, 7½ MILES
FROM HEADWORKS—A FEW YARDS OFF THE MAHANT'S HIGH SCHOOL.
1 + + + — + —+ III
......
Group I, n i l .
2 + — + — + — — IV B. Lactis ærogenes ...
„
II, nil.
3 + — + — + — + IV B. No. 102 ...
...
„
III, 6.
4 + — + — + — — IV B. Lactis ærogenes ...
„
IV, 4.
(a ) Total colonies on agar at 37° — countless.
5 + + + — + + + III
......
+
—
6 + + — — + — — III
. . . . . .
(b) Bile salt lactose, medium.
7 + + —— + +
—
I I I
......
8 + — —— + — + IV B. Cloacæ.
1 tube 20 cc. of water taken ... ...
1
...
9 + + — — + — — III
2 tubes 10 cc.
„
...
2
...
10 + + — ——— — III B. Neapolitanus.
4
„ 5 c c . „
4
...
5
„
1
cc.
„
...
5
...
5
„
.
1 cc.
„
,,
...
...
1
4
5 „ .01 cc.
„
...
...
5
(c) Fœcal bacilli in 1 cc. and upwards
.
(d) S porogenes milk test — in 20 cc.
24