197
TIRUPATI
SAMPLES TAKEN—21ST NOVEMBER 1909.
REMARKS.—The Kalyani river is dry. The supply is constant. There has been about 6 . 55 inches of rain since last analysis.
Bacteriological Analyses.
SAMPLE I.—A SHALLOW WELL ON THE BANK OF KALYANI RIVER TO
THE EAST OF HEAD WORKS (UNFILTERED WATER).
C o l o n y n u m b e r .
L a c t o s e .
S a c c h a r o s e .
D u l c i t e . A d o n i t e . I n u l i n .
V o g e s a n d P r o s k .
I n d o l .
M o t i l i t y .
G r o u p .
Bacillus.
Groups and
Remarks.
(a) Total colonies per cc. on agar at 37°—1,230.
+
—
( b) Bile salt lactose, medium.
1
+ + - + - + + + IV
B . 1 1 5 . . .
Group I, 1.
1 tube of 20 cc. of water taken ..
..
1
. .
2 tubes of 10 cc. ,, .. ..
2
. .
„ II, nil.
2 + + - - - + - + IV B. Cloacæ ..
..
3 ,, of 5 cc.
,,
..
..
3
..
B. 77
..
..
„ III, 2.
3 + + + + - + + + III
3 ,, of 1 cc.
,,
..
..
3
. .
4 + + + + - + - + III
1
. .
3 ,, of
. 1
c c .
,,
..
..
B. 115
..
.. „ IV, 7.
5 + + - + - + + + IV
3 „ of .01 cc.
,,
..
..
1
..
B. Cloacæ..
..
6 + + - - - + - + IV
Class I, nil.
7 + + - - - + + + IV B. 113
. „ II, 1.
(c) Fœcal bacilli in •1 cc. and upwards.
B. Cloacæ..
..
8 + + - - - + - + IV
„ III, 4.
9 + + - + - + - - IV B Lactis ærogenes .. Undetermined 5.
10 + - - - - - - - I B. Mutabilis.
SAMPLE II .— A SHALLOW WELL ON THE BANK OF KALYANI RIVER TO
THE WEST OF HEAD WORKS (UNFILTERED WATER).
1
+
+
- + - + - + IV B. 102
..
.. Group I, nil.
(a) Total colonies per cc. on agar at 37°—countless.
2 + +
- + - + - - IV B. Lactisærogenes ..
„ II, nil.
(b) Bile salt lactose, medium.
+
-
3 + + -
+
- + - + IV
Do.
..
„III, 2.
4 + + + + - + - - III
5 + + - + - + - - IV B Lactis ærogenes ..
„ IV, 8.
1
tube of 20 cc. of water taken . . . .
1
..
Do.
..
6 + + - + - + - - IV
Class I, nil.
7 + + + + - + - - I I I B. 67
..
..
„ II, 9.
2
2 tubes of 10 cc.
, , . . . .
. .
8 + + - + - + - - IV B Lactis ærogenes .. „ III, nil.
Do.
..
3
. .
9 + + - + - + - - IV
3 „ of 5 cc.
,,
..
..
Undetermined 1.
3 „ of 1 cc.
,,
..
..
..
Do.
3
10
+
+ - + - + - - IV
3
. .
3 ,, of • 1 cc.
,,....
..
3 „ of .0 1
c c .
,,
..
..
1
2
(c) Fœcal bacilli in .1 cc. and upwards.
SAMPLE III.—COLLECTING CHAMBER—FILTERED WATER.
1
+
+ - - - + - + IV B. Cloacæ ..
.. Group I, nil.
(a) Total colonies per cc. on agar at 37°—4,680.
+
-
Do.
..
. .
2 + + - - - + - + IV
„ II, nil.
(b) Bile salt lactose, medium.
3 + + - - - + - + IV
Do.
.. ,, III, nil,
Do.
.. ..
4 + + - - - + - + IV
1
tube of 20 cc. of water taken ..
..
1
..
„ IV, 10,
5 + + - - - - - + IV B. 109.
2 tuhes t
10
of cc .
, , . . . .
2
B. Cloacæ..
..
..
6 + + - - - + - + IV
Class I, n i l
3 ,, of 5 cc.
,,
..
3
. .
Do.
..
..
7 + + - - - + - + IV
,, II, nil.
3
. .
3 ,, of 1 cc.
,, .. ..
8 + + - - - + - + IV
Do.
..
.. „ III , 10.
3
Do.
3 „ of .1 cc.
,, ..
9 + + - - - + - + IV
..
Do.
3 „ of .01 cc.
,,
..
..
2
.. 10 + + - - - + - + IV
(c) Fœcal bacilli in • 01 cc. and upwards.
SAMPLE IV.—A TAP IN WESTERN CORNER IN THE MIDDLE OF THE
KARNALA STREET .
1 + +
- - - - -
+ IV B. 109
..
.. Group I, nil.
(a )
T o t a l c o l o n i e s p e r c c
.
o n a g a r a t 3 7°— 2 , 3 6 0 .
+
-
2 + + + - - - - + III B. 74
..
..
„ II, nil.
(b) Bile salt lactose, medium.
3 + + - - - - + + IV B. 106
. . . .
„ III, 3.
1 tube of 20 cc. of water taken ..
..
1
..
B. 109
..
..
4 + + - - - - - + IV
,, IV, 7.
5 + + + - - + - + III B. 73.
2 tubes of 10 cc.
, , . . . .
. .
6 + + - - - - + + [V B. 106
..
..
2
Class I, nil.
3 ,, of 5 cc.
,,
..
..
3
7 + + - -
- - - + IV
. .
B. 109
..
.. ,, II , 10.
8 + + + - - + - + III B. 73
..
..
3
, , of 1 ce.
, , . . . .
3
. .
(Mixture of 5)
1
3
,, of .1 cc.
, , . . . .
2
9 + + - - - - - + IV B.109.
III, n i l
2
3
„ of .01 cc.
,,
..
..
1
B. 109.
10
+
+
- - - - -
+ IV
(C) Fœcal bacilli in .01 cc. and upwards.
50