28
TABLE V.
RABICIDAL PROPERTIES OF SERUM.
Experiments on rabbits to test the rabicidal properties of the serum of animals immunised with
dead rabies virus.
No. of ex-
periment and
animal im-
munised.
Preparation of
dead rabies virus
used for
immunising.
Time which
had elapsed.
after com-
pletion of
treatment
when serum
was tested.
Proportion of serum and
living virus tested, also
time and temperature at
which kept.
Test applied to
mixture of serum
and virus.
Result.
REMARKS.
1. Monkey. 4 per cent. rabies
virus in 0.5
per cent. carbo-
lic acid.
11 days
Equal parts serum and, 1
in 300 " fixed virus,"
kept 2 hours at 37°C.
Rabbit inocula-
ted subdurally
with 0.2 c.c.
Remained,
well.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal mon-
key's serum and 1 in 300
" fixed, virus," kept for
2 hours at 37°C developed
rabies on the 7th day.
2. Monkey.
Ditto.
. 11 days .
Ditto ditto.
Ditto
. . Rabies,
10th day.
Ditto. ditto.
3. Monkey.
Ditto
. 14 days . Equal parts serum and 1
in 200 1st passage human
virus, mixed and kept 2
hours at 37°C
Ditto
. Rabies,
17th day.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal mon-
key's serum and 1 in 200
1st passage human virus
kept for 2 hours at 37°C
developed rabies on the
10th day.
4. Monkey.
Ditto
. 14 days .
Ditto ditto.
Ditto
. Remained
well.
Ditto. ditto.
5. Dog
Ditto
. 11 days . Equal parts serum and 1
in 300 " fixed virus "
kept 2 hours at 37°C.
Ditto
.
Ditto.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal dog's
serum and 1 in 300 "fixed
virus " kept for 2 hours
at 37°C developed rabies
on the 7th day.
..
....
23 days . Equal parts serum and 1
in 300 2nd passage jac-
kal's virus mixed and
kept for 2 hours at 37°C.
Ditto
. Rabies,
15th day.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal dog's
serum and 1 in 300 2nd
passage jackal's virus
kept 2 hours at 37°C
developed rabies on the
11th day.
6. Dog
Ditto
. 11 days . Equal parts serum and 1
in 300 " fixed, virus "
mixed and kept for 2
hours at 37°C.
Ditto
. Remained
well.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal dog's
serum and 1 in 300
"fixed virus" kept for 2
hours at 37°C developed
rabies on the 7th day.
..
....
23 days . Equal parts serum and 1
in 300 2nd. passage jac-
kal's virus, mixed and
kept 2 hours at 37°C.
Ditto
. Rabies,
15th day.
A c o n t r o l r a b b i t i n o c u l a t e d
subdurally with 0.2 c.c.
equal parts normal dog's
serum and 1 in 300 2nd
passage jackal's virus,
kept for 2 hours at 37°C
developed rabies on the
11th day.
7. Rabbit. 2 per cent.
virus in 0.5 per
cent carbolic
acid.
8 days
. Equal parts serum and 1
in 200 " fixed, virus,"
kept 2 hours at 37°C.
Ditto
. Remained
well
.
A control rabbit inoculated
subdurally with 0.2 c.c.
equal parts normal rab-
bit's serum and 1 in 200
fixed " virus kept 2
hours at 37°C developed
rabies on the 7th day.
. .
....
25 days .
Ditto ditto
.
Ditto
.
Ditto.
Ditto. ditto.
8. Rabbit.
Ditto
. 8 days .
Ditto ditto
.
Ditto
.
Ditto.
Ditto. ditto.
. .
....
25 days .
Ditto ditto
.
Ditto
. Rabies,
10 th
day.
Ditto. Ditto.
All these experiments with the serum from immunised rabbits, dogs, and, monkeys, were controlled with serum from normal rab-
bits, dogs, and monkeys, and treated exactly the same after being mixed with the same dilutions of virus as the serum which was
being tested. All the control rabbits contracted rabies after the usual incubation period according to the virus used.