NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
42
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
" ordinary " vaccine, an "extracted" vaccine was prepared for trial
during the 1923-24 season, and also anti-serum against each, of the vac-
cines, to be administered simultaneously with the first dose of vaccine.
Each incoming batch of remounts admitted into the depot was to be
divided into three lots,—the first to be treated with a course of " ordinary"
vaccine, the second to be treated with " extracted" vaccine, and the
third to be left untreated as " true " controls. The " extracted " vaccine
was made up to the same strength in organisms as the "ordinary"
vaccine. On arrival at the admission camps, the young animals to be
treated were given a dose of vaccine and anti-serum, followed a week
later by an increased dose of vaccine, and again a week later by a more
increased dose. Animals that did not contract the disease on admission
to the main depot, nine days afterwards, were then injected at intervals
of one month with vaccine of the same kind and dosage as that given
at the third injection.
As in the 1922-23 season, strangles made an early appearance at
Mona, but the veterinary officer in charge decided to carry through the
original programme so that every vaccinated colt received the three
doses laid down in the treatment. At Sargodha, all colts except one
received the full three doses before they got strangles. The following
table represents the crude results obtained:—
1923-24 Season.
Depot
Sargodha
Mona
Treatment
" Extracted " vac-
cine.
" Ordinary " vac-
cine.
Controls "true"
" Extracted" vac-
cine.
" Ordinary " vac-
cine.
Controls " true "
No.
129
128
120
107
108
108
Re-
mained
free
66
(50.4%)
75
(50.9%)
53
(44.2%)
64
(59.8%)
45
(41.6%)
36
(33.2%)
Became
infected
63
(49.6%)
53
(41.1%)
67
(55.8%)
43
(40.2%)
63
(58.3%)
72
(66.8%)
SEVERITY OF AFFECTION
Mild,
recover-
ed
Severe,
recover-
ed
50
(39.5%)
44
(34.4%)
48
(40.0%)
35
(32.7%)
48
(44.4%)
53
(49.1%)
11
(8.5%)
5
(3.6%)
13
(10.8%)
7
(6.5%)
8
(7.4%)
12
(11.1%)
Died
2
1.6%)
4
(3.1%)
3
(5.0%)
1
(0.92%)
7
(6.48%)
7
(6.48%)
It has been stated that at Mona strangles made its appearance early
during the season, and hence a large proportion of the vaccinated animals
showed signs of infection before the completion of the treatment. The
MUKTESAR, FOR THE TWO YEARS ENDING THE 31 ST MARCH, 1924 43
development of infection at the depot is illustrated in the following
table:—
Number of animals
that developed
strangles after
1st dose
2nd dose
3rd dose and subsequently .
TREATMENT
"Extracted " vaccine
12 (deaths, nil)
16 (deaths, nil)
15 (death, 1)
" Ordinary " vaccine
14 (deaths, 4)
19 (death, 1)
30 (deaths, 2)
At Sargodha, most of the foals developed strangles after admission
into the main depot, i.e., after the 3rd dose of the initial treatment.
Several colts, however, were subjected to several inoculations before
they eventually developed strangles, but after the seventh injection
strangles disappeared and treatment was discontinued. The develop-
ment of infection after the successive injections is illustrated in the
following table :—
Number
of doses
3
4
5
6
7
Treatment
"Extracted" .
"Ordinary" .
"Extracted" .
" Ordinary " .
"Extracted" .
" Ordinary " .
"Extracted" .
" Ordinary " .
"Extracted" .
" Ordinary " .
Animals
clinically
affected
52
30
9
14
2
5
2
1
2
SEVERITY OF AFFECTION
Mild,
recovered
41
26
7
10
2
5
1
1
2
Severe,
recovered
9
2
2
3
..
..
..
..
..
..
Died
2
2
1
..
..
..
1
..
..
It is not proposed to discuss at legnth the value of the treatments.
This question will be dealt with in a technical paper now under prepara-
tion by Mr. Bennett, who was in charge of the execution of all technical
laboratory processes entailed in the research. The results displayed
above would appear to indicate that prophylactic vaccination exerts
an appreciable effect in depressing the incidence of morbidity and
44
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
mortality from strangles, and that continued treatment with the "ex-
tracted " form of vaccine yields superior results to those obtained with
a vaccine made in the " ordinary " way from dead streptococci. Ne-
vertheless, one is bound to admit that the efficacy of either form of in¬
tervention, when compared, for example, with the success obtainable in
the prophylaxis of rinderpest and bovine hæmorrhagic septicæmia, leaves
very much to be desired. Perhaps, the recent indications of Brocq¬
Rousseu, Forgeot, and Urbain upon the rapid antigenic changes that
take place in the strangles streptococci after isolation in artificial culture
media may afford a key to the relative inefficacy of the ordinary means
of prophylactic inoculation against strangles. Researches based upon
these conceptions are being continued at the laboratory. The infor-
mation now available in regard to the common methods of prophylaxis
would not have been available without the hearty and efficient cooper-
ation of Major W. N. Rowston, R.A.V.C., and Captain R. E. Bond,
R.A.V.C., Veterinary Officers in charge of the " country-bred " Remount
Depôts at Sargodha and Mona, respectively. The clinical data supplied
by these officers left nothing to be desired. Trials have also been made
at the breeding depôt at Ahmednagar, in collaboration with Major G.
W. Williamson, R.A.V.C., Veterinary Officer in charge, but the results
of the tests are still to be awaited.
(f) Contagious Equine Abortion. As stated in the last Annual report
of the laboratory, methods of controlling the common form of equine
contagious abortion, due to Bacillus abortivo-equinus, were taken up
for study. The application of the agglutination test to the breeding
animals upon the Government Cattle Farm, Hissar, Punjab, had
indicated that infection was serious and widespread, and hence an
attempt was made by periodic testing of all animals on the Farm, every
3 to 6 months, and the segregation of reactors, to stay the ravages of
the infection. The application of the test for over two years did not
enable us to attain the desired degree of control, but the prospects will
be stated more clearly in the next annual report. Systematic tests
were also conducted upon a number of other studs, notably two large
remount breeding studs in the Punjab and the Ahmednagar Remount
Depôt. It would appear that on one of these establishments the disease
is gaining a foothold, while on others the natural conditions for the spread
of infection limit the incidence of abortion to a small recurring, but
varying, annual percentage. The results of serological tests conducted
at the laboratory until the end of the period under report were:—
Year
1921-22 . . .
1922-23 . . .
1923-24 . . .
Positive
22
122
139
Suspicious
48
165
178
Negative
261
1168
1429
TOTAL
331
1455
1746
MUKTESAR, FOR THE TWO YEARS ENDING THE 31ST MARCH, 1924 51
into the micro-organisms responsible for affections of the very young
equines in India. From "joint-ill" material there was isolated Bac-
terium viscosum equi for the first time in this country ; from the joint
cavity of another specimen affected with this condition there was grown
a peculiar fusiform organism in pure culture ; from the joint cavity of
an aborted foetus there was isolated in pure culture a haemolytic strep-
tococcus, pointing, contrary to the available authoritative evidence,
to intra-uterine infection with this type of organism.
Numerous examinations were made upon an enzootic form of pleuro-
pneumonia affecting lambs in a certain district in Madras.
Exhaustive bacteriological tests were made upon a complicated
apparatus set up for the " hyperimmunization " of rinderpest serum
producers. These tests showed that massive contamination with pyogenic
organisms was inevitable owing to the insurmountable difficulty of pro-
viding for effective sterilisation of the tubular portions of the apparatus.
The simple procedures previously adopted involving the use of plain
easily sterilisable vessels were therefore resumed.
Tests were also made upon the efficacy of certain preservatives other
than carbolic acid for anti-sera. It was found that the addition of this
chemical in the usual half per cent. quantity did not prevent the rapid
growth of certain, relatively phenol-fast, organisms, notably staphy-
lococci. Ortho-cresol, pure, at 0.35 per cent. concentration, was,
however, the most effective of the series tried, but short of sterilising
the serum the addition of phenol preservative was found to have a very
marked effect upon the keeping qualities of the serum in antibodies.
Further tests upon the factors involved in the preservation of the anti-
body content of serum were under execution by Mr. Bennett at the close
of the period.
During the 1922-23 season the active immunization against rinder-
pest of the Pusa herd was undertaken by Mr. Bennett, and again at
the commencement of the 1923-24 season the Government Military
Dairy herds at Lahore and Ambala were inoculated by him ; the Luck-
now and Sitapur herds were inoculated by Mr. Haddow, and the herd
belonging to the Imperial Institute of Animal Husbandry and Dairying
at Bangalore and the herd at Wellington were inoculated by Mr. Cooper.
A considerable amount of information was collected in this manner upon
the factors that make for the safety of the method.
A few examinations were made at the laboratory upon " bursattee "
material, and although intense eosinophilia could be detected invariably
in specimens of young lesions, indicating infection of verminous origin,
the actual presence of a worm parasite was not discovered.
Specimens of tissues from cattle suspected to be affected with Johne's
disease were examined, with positive results.
Neoplasms, comprising carcinoma, adeno-carcinoma, round-celled
sarcoma, and papilloma, were diagnosed in the specimens forwarded
to the laboratory for examination. Specimens from a number of Army
" debilitated " horses revealed intense verminous infestation.
52
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
It was intended at the commencement of the period to prosecute
major researches upon Kumri in horses, and materials were collected
for the purpose, but pressure of other work precluded the execution of
anything more than preliminary experiments.
In the post-mortem houses the carcases of 2,222 animals were
systematically examined in 1922-23 (cattle 1,248, buffaloes 164, horses
15, donkeys 3, sheep 10, goats 7, dogs 83, cat 1, rabbits 676, guinea-
pigs 14, pigeon 1) and of 3,318 animals in 1923-24 (cattle 1,449, buffaloes
222, horses 15, mules 2, donkey 1, sheep 7, goats 6, dogs 8, rabbits 1,591,
guinea-pigs 13, rat 1, fowls 3). A special note of commendation must
be paid to Veterinary Inspector Mr. R. G. Sathe for the manner in which
he assisted Mr. Cooper in this work.
As will be seen from the amount of material available for study there
is a pressing need for the appointment of a whole-time helminthologist
upon the staff of the laboratory. A resolution was passed by the Second
Meeting of Veterinary Officers in India at Calcutta in February—March
1923 urging the economic necessity of appointing a specialist in this branch
of zoological study for the benefit of veterinary workers in India. Some
experiments were carried out by Mr. Cooper in 1922-23 upon the efficacy
of certain authelmintics in treatment of worm infections in dogs.
V. CONCLUSION.
In the course of the text of this report mention has been made of the
names of individuals who have undertaken the execution of certain works
directed to them, but conspicuous service, even to the purely research
side of the work, has been rendered by all other men employed at the
laboratory and who now number on an average 895 souls. It has been
a great privilege to direct the energies of men imbued with so much
willingness and gentle manners. Special mention must be made of the
whole office staff, superintended by Rai Sahib Pandit Krishna Nand,—
whose great experience and tact have been an invaluable asset in the
peculiar and difficult administration of the institution. During his
absence on sick leave from 1st September, 1923, to 31st March, 1924,
his place was taken by Pandit Buddhi Ballabh Joshi, who performed
the duties in a quiet and most effective manner. Munshi Azimushan
Khan (2nd Clerk), Babu Godarmal Lamba and Pandit Ram Datt
Joshi (Accountant) deserve much credit for their work, and Babu Sri
Ram Saxena for his services as clerk on my tours. In the conduct of
work at the branch laboratory at Izatnagar, Munshi Abdul Ghani,
through his ability to command labour and to obtain the needs of the
laboratory from the locality, has rendered most meritorious service. In
the routine work of serum production at Muktesar Khan Sahib Wazir
Mohammed has been the mainstay of the outside duties. In the pre-
paration of vaccines and other products Mr. D. Keiller has borne heavy
responsibility and he could always be relied upon to discharge effectively
the exceptionally arduous new tasks allotted to him; he also took a
MUKTESAR, FOR THE TWO YEARS ENDING THE 31 ST MARCH, 1924 53
leading part in the administration of the laboratory during my absence
on tour in the cold weather season 1923-24. The multifarious tasks
entrusted to Mr. W. Pryde Watson have been dwelt upon earlier in the
report, and many important schemes of research would have been im-
possible of execution without his ingenuity. Veterinary Inspectors
P. C. Banerji and G. R. Viswanathan rendered highly intelligent assis-
tance to the officers in charge of the research schemes of the laboratory-
The services of Mr. M. J. Brett in charge of outside duties as Veteri.
nary Officer and his genial manner proved to be an asset of inestimable
value to the institution.
During the period under report the laboratory was visited by, among
other visitors, (i) Sir William Edwards, Director General, Indian Medical
Service, on 8th June 1922. (ii) Mr. S. Milligan, Agricultural Adviser
to the Government of India, on 11th March 1923. (iii) Colonel W. Pallin,
Assistant Director, Veterinary Services, Eastern Command, on 9th
June 1922. (iv) Mr. M. S. D. (now Sir Montagu) Butler, Secretary to
the Government of India, Department of Education, Health and Lands,
on 25th October, 1923. (v) Colonel J. W. D. Megaw, I.M.S., Principal
of the Calcutta School of Tropical Medicine, on 22nd April, 1923. (vi)
The Deputy Commissioner, Naini Tal, on 18th October, 1923. (vii)
Sir Arnold Theiler, K.C.M.G., Director of Veterinary Education and
Research, Union of South Africa, on 7th February, 1924. (viii) M.
Schem, Veterinary Service, French Indo-China, on 27th January, 1924.
A great deal of invaluable information and advice was obtained from
these distinguished gentlemen in the course of their visits.
APPENDIX I-A.
Table showing a summary of results of inoculations in the field reported
from the use of different products issued from the Imperial Bacterio-
logical Laboratory, Muktesar, during the year 1922-23.
Province
Punjab.
Rinderpest serum .
Hæmorrhagic Septicæ-
mia serum.
Hæmorrhagic Septicæ-
mia vaccine.
Black-quarter vaccine .
Number of outbreaks in
which inoculated
298
309
755
87
NUMBER OF
ANIMALS WHICH
DIED UNINOCU¬
LATED IN COURSE
OF DISEASE
Equines
..
..
..
..
Bovines
3,432
2,853
..
..
Others
17
..
..
..
NUMBER OF ANIMALS
INOCULATED
Equines
..
..
..
..
Bovines
50,216
82,443
175,216
9,809
Others
654
..
..
..
NUMBER OF
ANIMALS
WHICH DIED
AFTER
INOCULATION
Equines
..
..
..
..
Bovines
284
65
..
..
Others.
..
..
..
..
Percentage of deaths in
inoculated bovines
..
..
..
..
54
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
APPENDIX I-A.—contd.
Table showing a summary of results of inoculations in the field reported
from the use of different products issued from the Imperial Bacterio-
logical Laboratory, Muktesar, during the year 1922-23.
Province
North-West Frontier
Province and North
Punjab.
Rinderpest serum
Hæmorrhagic Septicæ-
mia serum.
Hæmorrhagic Septicæ-
mia vaccine.
United Provinces.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ-
mia serum.
Black-quarter vaccine .
Bengal.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septice-
mia serum.
Assam.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septice-
mia serum.
Bihar and Orissa.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ-
mia serum.
Black-quarter vaccine .
Number of outbreaks in
which inoculated
11
2
107
321
1
108
4
584
4
29
196
50
30
834
15
245
6
NUMBER OF
ANIMALS WHICH
DIED UNINOCU¬
LATED IN COURSE
OF DISEASE
Equines
..
..
..
..
..
..
..
..
..
..
..
14
3
..
..
..
..
Bovines
101
17
..
4,047
15
857
72
10,108
24
875
3,176
187
263
6,508
59
1,488
23
Others
..
..
..
..
..
..
..
..
..
..
...
7
..
..
..
..
..
NUMBER OF ANIMALS
INOCULATED
Equines
..
..
..
..
..
..
..
..
1
..
..
273
4
..
..
...
..
Bovines
3,630
932
23,852
34,650
87
12,665
1,000
111,756
84
6,859
16,783
1,699
3,237
113,190
1,206
42,729
2,200
Others
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
NUMBER OF
ANIMALS
WHICH DIED
AFTER
INOCULATION
Equines
..
..
..
..
..
..
..
..
..
..
..
2
..
..
..
..
..
Bovines
6
..
..
58
..
4
..
788
..
14
144
6
1
573
2
38
13
Others
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Percentage of deaths in
inoculated bovines
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
MUKTESAR, FOR THE TWO YEARS ENDING THE 31 ST MARCH, 1924
55
APPENDIX I-A—contd.
Table showing a summary of results of inoculations in the field reported
from the use of different products issued from the Imperial Bacterio-
logical Laboratory, Mulctesar, during the year 1922-23.
Province
Central Provinces.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ-
mia serum.
Bombay.
Rinderpest serum
Anthraxserum . .
Hæmorrhagic Septicæ-
mia serum.
Hæmorrhagic Septicæ-
mia vaccine.
Black-quarter vaccine .
Madras.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ-
mia serum.
Black-quarter vaccine .
Sind and Rajputana.
Rinderpest serum .
Hæmorrhagic Septiæ-
mia serum.
Baluchistan.
Rinderpest serum
Anthrax serum .
Number of outbreaks in
which inoculated
86
13
31
93
14
35
6
1
130
10
37
1
51
7
3
2
NUMBER OF
ANIMALS WHICH
DIED UNINOCU¬
LATED IN COURSE
OF DISEASE
Equines
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Bovines
777
124
241
1,800
126
410
10
1,769
127
608
503
82
13
..
Others
..
..
..
..
..
..
..
..
..
..
..
..
..
86
NUMBER OF ANIMALS
INOCULATED
Equines
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Bovines
8,410
1,166
3,163
10,374
857
3,263
915
125
21,543
948
6,998
402
8,153
737
191
..
Others
..
..
..
..
..
..
..
7
..
48
..
..
..
..
1,060
NUMBER OF
ANIMALS
WHICH DIED
AFTER
INOCULATION
Equines
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Bovines
16
5
..
146
..
9
1
64
5
6
..
74
7
..
..
Others
..
..
..
..
..
..
..
..
..
1
..
..
..
..
1
Percentage of deaths in
inoculated bovines
..
..
..
...
..
..
..
..
..
..
..
..
..
..
..
56
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
APPENDIX I-A.-concld.
Table showing a summary of results of inoculations in the field reported
from the use of different products issued from the Imperial Bacterio-
logical Laboratory, Muktesar, during the year 1922-23.
Province
Burma.
Rinderpest serum
Bengal Veterinary Col-
lege.
Rinderpest serum
Hissar Cattle Farm.
Anthrax serum .
Hæmorrhagic Septicæ-
mia vaccine.
Black-quarter vaccine .
Mysore State.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ-
mia serum.
Black-quarter vaccine .
Total.
Rinderpest serum
Anthrax serum .
Hæmorrhagic Septicæ
mia serum.
Hæmorrhagic Septicæ-
mia vaccine.
Black-quarter vaccine .
Number of outbreaks in
which inoculated
270
628
1
..
1
423
35
50
501
3,928
145
883
868
601
NUMBER OF
ANIMALS WHICH
DIED UNINOCU¬
LATED IN COURSE
OF DISEASE
Equines
..
..
..
..
..
..
..
..
..
..
14
3
..
..
Bovines
3,294
1
3
1
1
4,764
924
571
2,874
41,293
1,589
8,265
1
2,980
Others
..
..
1
1
..
100
30
..
..
117
124
1
..
NUMBER OF ANIMALS
INOCULATED
Equines
..
..
..
..
..
..
..
..
..
274
4
..
..
Bovines
23,793
891
100
855
1,976
74,512
4,355
8,180
29,235
478,092
10,502
172,206
200,838
45,347
Others
..
31
..
..
214
794
..
..
906
1,854
48
..
..
NUMBER OF
ANIMALS
WHICH DIED
AFTER
INOCULATION.
Equines
..
..
..
..
..
..
..
..
..
2
..
..
Bovines
600
..
..
22
169
..
13
18
2,922
18
157
..
54
Others
..
..
..
..
338
4
..
..
338
5
1
..
..
Percentage of deaths in
inoculated bovines
..
..
..
..
..
..
..
..
0.61
0.17
0.09
0.00
0.11
MUKTESAR, FOR THE TWO YEARS ENDING THE 31ST MARCH, 1924 57
APPENDIX II-A.
Table showing amounts of various products prepared and issued from
the Imperial Bacteriological Laboratory, Muktesar, during 1922-23,
as compared with the average amounts of the previous 5 years.
Name of products
Rinderpest serum
.
.
.
.
Anthrax serum
Hæmorrhagic Septicæmia serum .
Hæmorrhagic Septicæmia vaccine
Black-quarter vaccine
Mallein
DOSES PREPARED
1922-23
2,000,661
65,118
202,714
273,100
63,000
21,279
Average of
previous 5
years
2,980,239
28,847
149,556
118,846
20,300
126,703
DOSES ISSUED
1922-23
1,937,552
34,496
226,998
273,100
57,550
13,484
Average of
previous
5 years
2,840,010
27,226
145,011
118,846
24,080
130,608
APPENDIX III-A.
Table showing the details concerning the nature of specimens, received and
examined at the Imperial Bacteriological Laboratory, Muktesar,
during the year 1922-23.
Nature of specimens
Piroplasmosis
Glanders
Black-quarter
Trypanosoma Evansi
.
.
.
.
Filaria
Fowl cholera
Foot and mouth
Hæmorrhagic Septicæmia
.
.
.
.
Epizootic Lymphangitis .
.
.
.
Tumours
Abortion Equine
Strangles
Necrosis
Nasal granuloma
.
.
.
.
.
Catarrhal pneumonia and pleurisy
Anthrax
Johne's Disease
.
.
.
.
.
Miscellaneous . . . . .
TOTAL . .
NUMBER OF SPECIMENS
Positive
5
2
..
1
1
2
19
..
..
2
122
5
..
1
1
5
..
20
186
Negative
10
1
2
..
..
..
..
1
6
..
1,168
2
..
..
..
4
1
14
1,209
Doubtful
2
..
..
..
..
..
..
..
..
..
165
1
5
..
..
1
..
8
182
58
REPORT OF THE IMPERIAL BACTERIOLOGICAL LABORATORY,
APPENDIX
Table showing the doses of different products issued from the Imperial
Products
Rinderpest serum . .
Anthrax serum . .
Hæmorrhagic
Septicæ-
mia serum.
Hæmorrhagic Septicæ-
mia vaccine.
Black-quarter vaccine .
Mallein . . .
Concentrated mallein .
Tuberculin . . .
Streptococcic serum .
Streptococcic vaccine .
Streptococcic and Sta-
phylococcic
(mixed)
vaccine.
Special vaccine . .
Autogenous vaccine .
Abortion vaccine . .
TOTAL .
QUANTITY OF SERA
Punjab
23,000
..
32,000
75,800
..
20
..
..
..
..
..
..
..
130,820
N.W.F.
P. and
North
Punjab
....
..
5,000
89,000
1,400
50
..
..
..
..
..
..
..
95,450
South
Punjab.
154,000
600
39,500
89,300
14,200
62
..
..
..
..
..
..
..
297,662
United
Pro-
vinces
108,100
1,033
22,900
..
3,500
52
..
..
..
..
..
..
..
135,585
Bengal
220,000
3,000
21,500
..
..
..
..
..
..
..
..
..
..
244,500
Assam
75,000
4,000
4,000
..
..
..
..
..
..
..
..
..
..
83,000
Bihar
and
Orissa
452,000
1,000
68,000
..
..
6
..
..
..
..
..
..
..
521,006
Central
Pro-
vinces
60,000
3,000
4,500
..
..
..
..
..
..
..
..
..
..
67,500
MUKTESAR, FOR THE TWO YEARS ENDING THE 31ST MARCH, 1924 59
IV-A.
Bacteriological Laboratory, Muktesar, during the year 1922-23.
IN DOSES
Bombay
15,000
1,320
6,000
2,500
200
1,500
..
..
..
..
..
..
..
..
26,520
Madras
57,100
1,800
8,000
..
1,000
406
..
65
..
..
..
..
..
..
68,371
Sind and
Rajpu¬
tana
37,000
..
1,000
..
..
..
..
50
..
..
..
..
..
..
38,050
Balu¬
chistan
1,400
600
..
..
..
24
..
..
..
..
..
..
..
..
2,024
Burma
70,000
..
..
..
..
100
..
..
..
..
..
..
..
..
70,100
Coorg
10,800
1,100
500
..
..
..
..
..
..
..
..
..
..
..
12,400
Military
Depart¬
ment
43,813
100
816
..
220
10,020
..
41
1,700
3,840
6
..
..
168
68,724
Native
States
289,090
6,938
12,017
16,500
37,000
794
..
286
100
..
29
24
26
..
362,804
Foreign
Coun¬
tries
277,640
9,999
..
..
..
10
390
..
..
..
..
..
..
..
288,039
Impe-
rial
Bacte-
riolo-
gical
Labo-
ratory
43,609
6
1,265
..
30
50
..
..
..
..
..
..
..
..
44,960
TOTAL
1,937,552
34,496
226,998
273,100
57,550
13,094
390
442
1,800
3,840
35
24
26
168
2,549,515