J. F.
SHIRLAW
303
Puppy No. 16 was subcutaneously inoculated on 1st August 1935 with
5 c. c. of a normal saline suspension of spleen and bone marrow from Puppy
No. 11, which showed numerous, if periodic, P. gibsoni in its blood during
life. This case was observed for eighty-six days. On the thirteenth day,
the normal incubation period of a P. gibsoni infection, there was a mild up-
ward trend to a slightly higher temperature level and in the first half of the
second month, the temperature fluctuated between 103—104°F., with P.
gibsoni absent. Anaemic changes set in about this period and continued pro¬
gressively until the end, when the haemoglobin percentage had fallen to thirty
per cent and a gross haemocytological picture was present. On the seventy¬
sixth day, until death on the eight-sixth, P. gibsoni was evident although some-
what scantily. At post mortem the liver and spleen were enlarged (unfortu-
nately these organs were not weighed) and the bone marrow noted as being
markedly hyperaemic. Acute interstitial myocarditis was noted. The spleen
was markedly diffluent. The histopathological changes affecting the spleen,
bone marrow and liver were those described in ' Lahore Canine Fever '.
No particular disease syndrome was noted in Puppies Nos. 16, 15, and 13
save towards the end when debility accompanied the profound secondary
anaemia.
The case of Puppy No. 10 (Chart 3) was, however, of a different category
and here there was a marked syndrome corresponding very closely to that of an
authentic 'Lahore Canine Fever'. The temperature reaction was not so
pronounced, but the symptomatology was identical. Puppy No. 10 was in-
oculated subcutaneously with 4 c. c. of citrated blood containing P. gibsoni and
observations were extended over fifty-two days when death, occurred. There
was a slight rise of temperature on the twelfth day after inoculation, becoming
very definite on the twentieth day. From the twenty-first day to the twenty-
fourth, P. gibsoni was found in numbers in the blood, and not again during
the course of the illness. Secondary anaemia was noted on the thirty-second
day with 70 per cent haemoglobin which dropped steadily to 30 per cent four
days before death.
The post mortem and histopathological examinations were similar to those
seen in Puppy No. 16 (Chart 7).
Puppies Nos. 6 and 7 which were selected for experimental treatment, are
further examples of cases, which showed febrile reactions with slowly develop-
ing and progressive secondary anaemia with erratic and scanty presence of P.
gibsoni in the blood during these developments. The charts of these two dogs
prior to treatment are given to illustrate the point (Charts 1 and 2). Puppy
No. 6 was inoculated on 9th February 1934 with 2 c.c. virulent blood. Eighteen
days after inoculation, the temperature rose three degrees and slowly abated
to normal on the thirty-first day, when, after repeated examinations, only one
P. gibsoni could be seen in the blood films examined. The haemoglobin per-
centage had, at this date, fallen to 70. At this stage, secondary anaemia was
evident. The parasite did not reappear until the forty-fourth day, when it was
very scanty. The following two days it was definitely absent. On the