S. R. HASSAN AND J. A. IDNANI 63
TABLE II
Primary transmission experiments
Source of maurial
Cow 100 . . .
Cow 100 and Bullock
136
Bullock 1543 .
Cow 172 . .
Cow 100 . .
Bullock 136 .
Cow 91 . .
Cow 106 . . {
Cow 142 .
.
{
Bullock 114 .
Buffalo 296 .
Cow 82 . .
Cow 153 .
.
Cow 74
.
.
Cow 56 . .
Cow 40
.
.
Pony 5B .
Inoculated to
Rabbit No. 1 .
Bull-calf No. 7
Hill bull 530 .
Hill bull 65
.
Hill bull 69
.
Hill bull 68 .
Hill bull 404 .
Hill bull 143 .
Rabbit 2 .
Rabbit 3.
Rabbit 4 . .
G. pig l . .
G. pig 2 . .
G. pig 3 .
Mouse 1 .
G. pig 4 .
Rabbit 5 .
Rabbit 6 .
Kabbit 7 .
G. pig 5 .
G. pig 6 .
Rabbit 8 .
Rabbit 9
G. pig 7 .
G. pig 8 .
Rabbit 10 .
Rabbit 11 .
G. pig 9 .
G. pig 10 .
G. pig 11 .
G. pig 12 .
G. pig 13 .
G. pig 14 .
Rabbit 12 .
Rabbit 13 .
Bull-call 35 .
Rabbit 14 .
Rabbit 15 .
Rabbit 16 .
Rabbit 17 .
Rabbit 18 .
Rabbit 19 .
Rabbit 20 .
Details of inoculation
Medulla, 1 per cent susp., 0.1 c.c
intrathecally
Liver, splcen, kidney and urine, mixed
susp., 5.0 c.c. subcutaneously and
brain susp.intranasally
Brain, medulla and cord. mixed susp.,
5.0 c.c. subcutaneously
Ditto . . .
Ditto . . .
Brain, medulla and cord, mixed
susp., 5.0 c.c. intranasally
Ditto . . .
Ditto . . .
Brain, medulla and cord, mixed
susp., 0.2 c.c. subdurally
Brain, medulla and cord, mixed
susp., 0.5 c.c. intranasally
Brain, medulla and cord, mixed
susp., 0.1 c.c. intracerebrally
Brain, medulla and cord, mixed
susp., 0.2 c.c. subdurally
Brain, medulla and cord, mixed
susp., 0.5 c.c. intranasally
Brain, medulla and cord, mixed
susp., 0.1 c.c. intracerebrally
Brain, medulla and cord, mixed
susp., 0.2 c.c. intranasally
Cord susp., 0. 1 c.c. subdurally .
Medulla susp., 0 . 1 c.c intrathecally .
Cord susp., 0. 1 c.c. subdurally .
Ditto . . .
Ditto . . .
Ditto . . .
Ditto . . .
Ditto . . .
Ditto . . .
Ditto . . .
Ditto . . .
Brain susp., 0.1 c.c subdurally .
Ditto . .
Cord susp., 0.1 c.c subdurally .
Ditto . . .
Cord susp., 01 c.c. subdurally
Ditto . . .
Ditto . . .
Ditto . . .
Medulla susp., 0.1 c.c intrathecally
Medulla susp. 0.1 c.c. subdurally .
Blood total 40.0 c.c. subcutaneously
Brain susp., 0.1 c.c. subdurally .
Ditto . . .
Blood, 10.0 c.c subcutaneously .
Ditto . . .
Brain and cord, mixed susp., 0.1 c.c.
subdurally
Urine, 10.0 c.c. subcutaneously, blood
10.0 c.c. intranasally
Urine, 10.0 c.c subcutaneously
Date
17-12-38
16-12-38
20-12-38
20-12-38
20-12-38
20-12-38
20-12-38
20-12-38
21-12-38
21-12-38
21-12-38
21-12-38
21-12-38
21-12-38
21-12-38
27-12-38
19-12-38
27-12-38
30-12-38
30-12-38
30-12-38
30-12-38
2-1-39
2-1-39
2-1-39
2-1-39
5-1-39
5-1-39
1-1-39
1-1-39
6-1-39
6-1-39
6-1-39
6-1-39
25-1-39
25-1-39
21-1-39
to 24-1-39
9-2-39
9-2-39
28-2-39
28-2-39
1-3-39
1-3-39
1-3-39
Result
D 13
D 22
No reaction
D 113
No reaction
Do.
Do.
Do.
Do.
Dest. 17 ; paralysis
No reaction
D 6
No reaction
D 33
D 33
D 18
D 11
After first inoculation
D 122
D 38
D 29
D 12
D 80
D 80
No reaction
D 30
No reaction
Do.
D 20
D 29
D 23
D 32
D 68
D 41
No reaction
D 21
No reaction
D 22
No reaction
Do.
Do.
D 30
No reaction
Do.
Susp. = suspension
D=died on
day
TABLE III
Serial inoculations with natural encephalomyelitis material
Source
of
material
Rabbit
1
(vide Table
II)
B. calf 7
(vide Table
II)
Inoculated to
Rabbit 21
Rabbit 22
B. calf 19
.
B. calf 25 .
Passage
No. from
original
material
2
2
2
Details of inoculation
Brain and cord, mixed susp.,
0 1 c.c. subdurally
Medulla susp., 3.0 c.c. sub-
cutaneousiy
Medulla susp., 3.0 c.c. and
0.5 c.c. intranasally
Date
30 December,
1038
10
January,
1939
10
January,
1939
Result
No reaction
D 30
D 36