48
the liver or the spleen in any case, nor from the blood in the case of No. 1 experi-
ment.
The tetanus bacillus isolated from the seat of injection of quinine in these
three monkeys was virulent, and killed guinea pigs in small doses (½ c.c. of a 14
days' growth killed guinea pigs in two days ; less would have sufficed).
Table XII.
Experiment on Monkeys with "Washed Tetanus Spores " and Quinine.
No of
experiment.
Date and seat
of injection of
½ c.c. spores.
Date and seat
of injection of
quinine.
Result.
RESULT OF AN ÆROBIC CULTIVATIONS MADE FROM
R E M A R K S .
Seat of injection
of spores.
Seat of injection
of quinine. Lever. Spleen.
1
2nd September
1910, right
hind leg.
2nd September
1910, 3 grs.,
chest. 3rd
September, 4
grs., chest.
8th Septem-
ber, 4 grs.,
chest.
Tetanus, 19th
September.
Died, 26th
September.
Growth of teta-
nus bacilli.
Growth of teta-
nus bacilli.
Nil.
Nil. Quinine com-
menced same
day as spores.
Lockjaw, an
early symp-
tom, followed
by stiffness of
neck and
shoulder
muscles, and
difficulty in
swallowing.
2
2nd September
1910, right
hind leg.
3rd September
1910, 4 grs.,
chest. 4th
September, 4
grs., chest.
8th Septem-
ber, 4 grs.,
chest.
Tetanus, 17th
September.
Died, 20th
September.
Growth of teta-
naus bacilli.
Growth of teta-
nus bacilli.
Nil.
Nil. Quinine com-
menced, one
day after
spores. Lock-
jaw, an early
symptom, and
soon after
tetanus
became gene-
ral and very
acute.
3
3rd September
1910, right
hind leg.
2nd September
1910, 3 grs.,
chest. 3rd
September, 4
grs., chest. 8th
September, 4
grs., chest.
Tetanus, 15th
September.
Died, 21st
September.
Growth of teta-
nus bacilli.
Growth of teta-
nus bacilli.
Nil.
Nil. Quinine com-
menced one
day before
spores. Lock-
jaw, an early
sympton, then
slow' general
tetanus set in,
finishing up
with empros-
thotonos.
4 (Control
of spores
used.)
2nd September
1910, right
hind leg.
..
Remained well.
5 (Control
of teta-
nus cul-
ture used
to pre-
pare
spores.)
2nd September
1910, 1 c.c.
tetanus cul-
ture, right
hind leg.
..
Tetanus, 4th
September,
Died, 5th
September.
Growth of teta-
nus' bacilli,
..
Nil.
Nil. Lockjaw, an
early and pro-
minent symp-
tom. Opistho-
tonos marked
before death.
I n N o . 1 e x p e r i m e n t , i m m e d i a t e l y a f t e r d e a t h , a g u i n e a p i g w a s i n j e c t e d s u b c u t a n e o u s l y w i t h 3 c . c . o f b l o o d t a k e n f r o m t h e h e a r t ,
but the animal did, not contract tetanus. An anærobic cultivation made from ½ c. c. of the heart ' s blood transferred to a broth tube
also gave negative results.
It is evident om the results of the experiments recorded in this table that quinine when given hypodermically to monkeys pro-
duces conditions in which pure "washed tetanus spores" become infective, and capable of producing fatal tetanus. It will be ob-
served that lockjaw was an early and prominent symptom in all four cases, and that opisthotonos was present in one case, and empros-
thotonos in another.
THE 13TH SERIES OF EXPERIMENTS.
The possibility of tetanus spores remaining alive and localised at the seat of
a former injury is of the utmost importance in relation to the hypodermic adminis-
tration of quinine. It will be noticed that in Tables I, II, III, IV, V, VI. X, XI,