41
THE 9TH SERIES OF EXPERIMENTS.
The object of this series of experiments was to prove whether quinine when
given in tetanus infections, in addition to producing a local site to which the infec-
tion becomes transferred, would also give rise to conditions in which infection
would be found in the internal organs.
Three guinea pigs were taken, and each was injected subcutaneously in the
hind leg with a tetanus culture, and at the same time each was given 1 grain of
quinine subcutaneously on the front of the chest. On the next day local tetanus
appeared in all three animals, and death took place two days later.
Immediately after death, anærobic cultivations were made from the seat of
injections of spores, seat of injection of quinine, and from the liver, and the spleen.
A growth of tetanus bacilli took place in all the cultivations made from site of
spores, and site of quinine, but not from the liver and the spleen.
The control guinea pig which received ¼ c.c. tetanus culture only, developed
tetanus, and died about the same time as the others. Anærobic cultivations made
from the site of injection of quinine and site of injection of tetanus culture grew
tetanus bacilli, but the liver and the spleen gave negative results.
This series of experiments in addition to confirming the results of the anærobic
cultivations recorded in Table VIII, also proves that quinine has no influence in
transferring the infection of tetanus from a localised site to the internal organs,
although this influence is marked in transferring infection from a localised site to
the site of injection of quinine.
Table IX.
Experiments on Guinea Pigs, to prove whether Quinine, in addition to producing favour-
able local conditions for the growth of the Tetanus bacillus, has any influence in
producing conditions in which the bacillus may be found in the internal organs.
No. of
experiment.
Date and seat
of injection
of tetanus
culture.
Date and seat
of injection
of quinine.
Result.
RESULT OF AN ÆROBIC CULTIVATIONS MADE FROM
REMARKS.
Seat of in-
jection of
tetanus
culture.
Seat of
quinine
injection.
Liver.
Spleen.
1
30th August
1910, right
hind leg.
30th August
1910, 1 gr. on
front of chest.
Tetanus, 31st
August.
Died, 2nd
September.
Growth of
tetanus
bacilli.
Growth of
tetanus
bacilli.
Negative Negative Tetanus bacilli were
cultivated from
seat of injection of
culture, and from
seat of injection of
quinine, but not
from the liver or
the spleen.
2
Ditto
.
Ditto .
Ditto .
Ditto .
Ditto . Ditto . Ditto .
3
Ditto .
Ditto .
Ditto .
Ditto .
Ditto . Ditto . Ditto .
4 Control of
tetanus
Cu1ture
used.
Ditt o
.
Nil.
Ditto .
Ditto .
..
Ditto . Ditto . Tetanus bacilli were
cultivated from seat
of injection of cul-
ture, but not from
the liver or the
spleen.
This series of experiments proves that the influence of quinine in producing local favouring conditions for the transference of teta-
nus baci l l i from t he seat of i nfect i on t o t he seat of qui ni ne nj ect i on, does not ext end t o produci ng condi t i ons i n whi ch t he baci l l us i s
found in the internal organs. The tetanus bacilli isolated from the seat of injection of quinine in this series of experiments were
virulent for guinea pigs.
G 2