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vesicle cicatrice. There were, however, not wanting cases in which the vesicles were
rather late in appearing—so many, sometimes, as two or even three days ; but once
formed, the course of the vesicles afterwards was perfectly regular, and all the dis-
tinctive characters well marked. It appeared as if the germinal matter contained in
the inserted lymph, though effective, was not sufficiently developed or advanced to
cause the appearance of the vesicles on the fourth day, but required two or three days
time to attain to that state. During the time that animal vaccination has been carried
on, I have had ample opportunities of testing the effectiveness of vaccination performed
with animal vaccine virus in protecting the system from a further vaccination with
human lymph, and, therefore, from an attack of small-pox. In some successful cases
of primary vaccination with animal lymph—on the fifth day of the operation, when the
vesicles had made their appearance just the day previous—more punctures were made,
and human vaccine lymph inserted therein. Vesicles made their appearance at the
sites of all the later punctures in the course of twenty-four or thirty-six hours, and after-
wards ran their course with the previously developed vesicles, in every respect, as if
they were all formed at the same time ; the later vesicles, resembling these first ones in
every characteristic save size, being considerably smaller, as if they had not time enough
to attain their usual size, thus showing that the system was already brought under the
influence of the lymph first used ; but the necessary changes in the system were not
completed, and time was still left for the later inserted lymph to act. The same thing
happened when the order of the lymph was reversed : human lymph being used
first, and the animal virus on the fifth day. In the same way, cross experiments
were made on the eighth day of the operation. Generally, no effects where produced.
In some a small, raised, red spot did make its appearance at the seat of the puncture ;
but this always faded away in the course of twenty-four or forty-eight hours, leaving
the bare marks of the punctures. Animal vaccination has, in my humble opinion, proved
itself to be a perfect success in Bombay ; and, as such, well worthy the fullest patronage
of both the Government, the Municipality, and the public at large. It is a matter for
congratulation that the whole community of Bombay has taken to animal vaccina-
tion very kindly. At the commencement there were a few objecting voices, strange
to say, from the European quarters chiefly. Two or three Parsees and one Jewess
objected to get their children vaccinated with animal lymph. This feeling of distrust
has now quite disappeared, and one and all now ask for animal lymph in preference
to that obtained from the human subject. At the commencement I had great appre-
hensions that the Bunyas and Bhattias would, on religious grounds, raise a voice of
opposition to animal vaccination, powerful in proportion to the extent of their
community, and on account of their containing very many individuals holding a high
and influential position. It is, however, gratifying that, instead of opposing it, they
were some of the first to accept it. Animal vaccination, whatever else it has achieved
or left undone, has proved a perfect boon to Bombay in one particular. The chief
burden of our reports, year after year, was the want of a sufficient quantity of good
lymph ; and this was owing to the general unwillingness shown by all the most learned,
intelligent, and informed, as well as the most ignorant and illiterate, to allow lymph to