NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
September 1951.]
AMRITAMOY
MUKHERJI
141
immunity test. As a routine practice all the animals were allowed only morning
grazing and were taken back to the shed before the sun was too hot.
Immunity test. Both the groups were tested for the resulting immunity three
months after their vaccination with 5 c.c. dose of virulent hill-bull blood virus keeping
one healthy hill-bull as control. The latter received the same dose of the virus
as the test animals. Reactions, if any, in both the groups were compared for any
evidence of the breakdown of immunity.
The experiment was over after the Immunity test.
(A) Experiment with goat tissue vaccine on hill-bulls
Fifteen hill-bulls were selected for the experiment and were inoculated subcu-
taneously each with 1 c.c. of 1 per cent saline emulsion of goat tissue vaccine in the
afternoon on 3 March, 1946.
TABLE I
Showing the grouping
Serial
number
1
2
3
4
5
6
7
TOTAL
Shade group
Hill bull
number
325
445
290
279
257
236
357
Group average
Colour
Black
„
„
„
„
„
„
Mean of 3
consecutive
days' Body
wt. in lb.
207
240
179
175
167
152
154
1274
182
Haemoglo-
bin gm.
(per 100 ml.
of blood)
61
7.1
61
6.9
6.9
7.6
7.0
47.7
6.8
Sun group
Serial
number
1
2
3
4
5
6
7
8
TOTAL
Hill bull
number
360
420
110
322
452
347
194
207
Group average
Colour
Black
„
„
„
„
„
„
„
Mean of 3
consecutive
days' body
wt. in lb.
206
241
179
172
169
193
168
135
1463
182.8
Haemoglo-
bin gm.
per 100 ml.
of blood)
6.4
6.1
6.9
6.0
5.5
7.5
6.8
6.1
51.3
6.4
142
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III.
TABLE II
(Showing the group average of body weight and blood constituents before vaccination and
after reaction period)
Group
B. W.
Mean of 3
consecutive
days
Hb .. gm
(per 100 ml.
of blood)
R. B. C.
millions per
c.m.m.
Percentage
of
C. V.
Ca
Inorg
P
Mg
Before vaccination
Shade Group .
Sun Group .
182
182.8
6.8
6.4
6.3
6.1
36.6
32.7
11.5
11.0
5.30
6.71
2.63
2.70
After reaction period
Shade Group .
Sun Group . .
180
175.5
6.5
6.4
5.4
4.9
30.1
31.9
12.6
11.6
6.45
6.24
2.50
2.84
Fig. 1. (Daily mean of 8 days' dry and wet bulb thermometer readings inside the shade from
4 to 11 March, 1946.)
September, 1951]
AMRITAMOY
MUKHERJI
143
Fig. 2. Daily mean of 8 days' dry and wet bulb thermometer readings in the exposed place
where 'Sun group' was exposed to the sun from 4 to 11 March, 1946.
Fig. 3. Daily mean of 8 days' body temperature of both the groups after vaccination from
4 to 11 March, 1946.
144
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II
[Vol. XXI, Part III.
TABLE III
Summary of clinical observations during the period of immunization, 4 to 13 March, 1946
Group
Shade group
Sun group
Serial
num-
ber
1
2
3
4
5
6
7
1
2
3
4
5
6
7
8
H. B.
number
325
445
290
279
257
236
357
369
420
110
420
452
347
914
207
Body
Tempera-
(O.F)
Highest, at
9 a.m.
during
the
period
of reaction
101.8
101.8
1010
100.4
99.8
101.8
101.4
101.8
101.6
100.6
102.4
101.4
101.8
102.8
102.0
Inappetence
Number
of days
feeds
fairly
3
..
..
..
..
2
..
..
..
..
3
3
3
6
3
Number
of days
off
feed
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Intestinal
disturbance
Number
of days
soft
faces
2
..
..
..
..
..
2
..
1
..
2
1
2
3
3
Number
of days
dia-
rrhoea.
2
..
..
..
..
..
..
..
..
1
2
..
1
2
Mouth
lesion
Number
of days
Vesicles
or ulcers
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Body wt. on lb.
Average
initial
Body
wt. lb.
207
240
179
175
167
152
154
206
241
179
172
169
193
168
135
Average
final
Body
wt. lb.
202
238
180
161
170
151
158
208
234
164
160
154
197
151
136
Differene
lb.
—5
—2
1
—14
3
—1
4
2
—7
—15
—12
—15
4
—17
1
Mortality
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Remarks.
Mild reactor
„ „
Non reactor
„ „
„ „
Mild reactor
„
„ „
„ „
Non
„
Satisfactory
reactor
„ „
„ „
„
„
„ „
September, 1951]
AMRITAMOY
MUKHERJI
145
Serum test for evaluation of potency
In accordance with the general procedure laid down in the beginning of this
article two composite mixtures of immune sera, one from the Shade group and the
other from the Sun group, were obtained and the potency test was done for two lots
of sera at the same time and under identical conditions. The results of the test are
given below:
'Shade group' serum : Gross potency . . .
'Sun group' serum :
„
„ . . . .
42.2 per cent
8.2 per cent
Mortality during the convalescent period and upto the immunity test
Hill-bull No. 257 of the Shade group having died of acute haematuria on 9-4-46,
there were only 6 hill-bulls of the Shade group and 8 hill-bulls of the Sun group
available for the immunity test.
TABLE IV
Summary of the results of the immunity test
Group
Shade group
Sun group
Serial
number
1
2
3
4
5
6
1
2
3
H. B.
number
325
445
290
279
236
357
369
420
10
Reaction
No reaction
„ „
„ „
„ „
„ „
„ „
„ „
Mild fever ; partial loss of
appetite
No Reaction
Result
Immune
„
„
„
„
„
„
Partially
immune
Immune
Remarks
Recovered
„
4
146
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III,
TABLE IV
Summary of the results of the immunity test—contd.
Group
Sun group
Serial
number
4
5
6
7
8
H. B.
number
322
452
347
194
207
Reaction
No Reaction
Mild fever ; partial loss of
appetite
No Reaction
High fever ; partial loss of
appetite; soft faeces
High fever, partial loss of
appetite ; soft faeces
Result
Immune
Partially
Immune
Immune
Partially
Immune
Partially
Immune
Remarks
Recovered
„
„
„
„
Two controls : Severe reaction. Both died on 9th day due to rinderpest.
Statistical results
TABLE V
Significance of the difference between the means of body weight and blood con'
stituents of both the groups before vaccination and after reaction period
Constituents
Kb
R.B.C.
C.V. per cent
C
P
Mg
Body wt.
Shade group
Significance of the diff-
erence between the
means of the consti-
tuents obtained before
vaccination and after
reaction period
Difference not significant
Difference significant
„ „
Difference highly signi-
ficant.
Difference not significant
„ „
„ „
Remark,
*
*
†
Sun group
Significance of the diff.
erence between the
means of the consti-
tuents obtained before
vaccination and after
reaction period
Difference not significant
Difference significant
Difference not signi-
ficant
„ „
„ „
„ „
Difference highly signi-
ficant
Remarks
*
*
*=In favour of group before vaccination
†=In favour of group after reaction period
September, 1951]
AMRITAMOY
MUKHERJI
147
(B) Experiment with goat tissue vaccine on buffaloes
TABLE VI
Showing grouping
Shade group
Serial
number
1
2
3
4
5
6
7
8
9
10
Buffalo
number
1256
1257
1258
1263
1266
1269
1270
1271
1274
1275
Colour
Black
„
„
„
„
„
„
„
„
„
Total
Group average
Mean of 3
consecutive
days B.W.
lb.
606
652
715
616
612
602
698
714
628
638
6481
648.1
Hb.gm.
per 100 ml.
of blood)
8.64
7.38
6.44
7.60
7.04
8.64
9.27
6.18
8.54
6.97
76.70
7.67
Sun group
Serial
number
1
2
3
4
5
6
7
8
9
10
Buffalo
number
1259
1260
1261
1262
1264
1265
1267
1268
1272
1273
Colour
Black
Total
Group average
Mean of 3
consecutive
days B.W.
lb.
664
622
605
552
729
596
706
665
584
782
6505
650.5
Hb. (gm.
per 100 ml.
of blood)
7.24
8.74
7.52
7.38
9.27
6.13
8.44
8.74
7.68
6.67
77.81
7.78
TABLE VII
Showing the group average of body weight and blood constituents of both the groups
before vaccination and after reaction period
Group
B. W. (Mean
of 3 conse-
cutive
days lb.)
Hb. (gm.
per 100
ml. of
blood)
R. B. C.
millions
per c.c.m.
Percentage
of C. V.
Mg per 100 ml. of serum
Ca
Inorg
P
Mg
Before vaccination
Shade group
Sun group
648.1
650.5
7.67
7.78
606
6.12
34.74
36.31
10.0
9.9
7.00
6.89
2.72
2.73
After reaction period
Shade group
Sun group
614.7
614.5
7.64
6.77
5.99
5.28
34.28
29.92
9.6
9.6
6.29
5.81
2.71
2.33
148
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II
[Vol. XXI, Part III,
Fig. 4. Daily mean of 8 days, dry and wet bulb thermometer readings inside the shed.
From 5 to 14 December 1946, excluding 8th and 9th December.
Fig. 5. Daily mean of 8 days, dry and wet bulb thermometer readings in the exposed place.
From 5 to 14 December, 1946, excluding 8th and 9th December.
September, 1951]
AMRITAMOY
MUKHERJI
149
Fig. 6. Daily mean of 8 days, body temperature of both the groups after vaccination.
From 5 to 14 December, 1946, excluding 8th and 9th December.
VACCINATION
All the 20 buffaloes were inoculated subcutaneously each with 1 c.c. of per
cent saline emulsion of goat tissue vaccine in the afternoon on 4 December 1946.
150
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III,
TABLE VIII
Summary of clinical observations during the period of immunization.
From 5 to 14 December 1946
Group
Shade group
Sun group
Serial
num
ber
1
2
3
4
5
6
7
8
9
10
1
2
3
4
5
6
7
8
9
10
Buffalo
bull
number
1256
1257
1258
1263
1266
1269
1270
1271
1274
1275
1259
1260
1261
1262
1264
1265
1267
1268
1272
1273
Body tem-
perature
(°F.)
Highest
at 9 a.m.
during
reaction
period
102.0
99.8
101.2
100.2
98.8
97.6
100.0
100.4
100.8
98.8
101.4
98.4
100.8
98.8
101.0
100.8
102.0
98.4
97.2
98.0
Inappetence
Number
of days
feeds
fairly
1
..
2
..
..
..
..
2
3
..
2
1
..
..
2
..
..
2
1
..
Number
of days
off
feed
..
..
3
..
..
..
..
1
1
..
1
2
..
..
..
..
..
1
..
..
Intestinal
Disturbance
Number
of days
soft
faeces
4
..
..
..
3
..
..
1
1
..
2
3
..
..
4
..
..
2
4
..
Number
of days
diarr-
hoea
..
..
5
..
..
..
..
4
5
..
2
2
..
..
..
..
..
3
..
..
Mouth
lesion
Vesicles
or
ulcers
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
..
Average
initial
B.W.
606
652
715
616
612
602
698
714
628
638
664
622
605
552
729
596
706
665
584
782
Body wt.
in lb.
Average
final
B.W.
596
639
Died on
611
612
589
676
655
539
616
635
594
554
534
688
571
679
619
550
721
Mortality
Difference
—10
—13
10th day
—5
nil
—13
—22
—59
—89
—22
—29
—28
—51
—18
—41
—25
—27
—46
—34
—61
Remarks
Mild reactor
Non-reactor
Severe reactor
Non-reactor
Mild reactor
Non-reactor
Non-reactor
Satisfactory reactor
Satisfactory reactor
Non-reactor
Satisfactory reactor
Satisfactory reactor
Non-reactor
Non-reactor
Mild reactor
Non-reactor
Mild reactor
Satisfactory reactor
Mild reactor
Non-reactor
N.B.—Buffalo No. 1260 died of debility on the 18th day after vaccination as a direct result of rinderpest
September, 1951]
AMRITAMOY
MUKHERJI
151
Serum test for evaluation of potency
The results are as follows:
Shade group serum:—Gross potency . . .
Sun group serum :—Gross potency . . . .
74 per cent
41.8 per cent
Mortality during the convalescent period and upto the immunity test
In the Shade group, 2 buffaloes, viz., 1271 and 1274, died of debility as a result
of reaction due to rinderpest, and buffalo No. 1269 died of acute tympanitis. Whereas,
in the Sun group, 4 buffaloes viz., 1259, 1260, 1267 and 1272 died of debility as a
result of reaction due to rinderpest, and 3 buffaloes, viz., 1261, 1265 and 1273 died of
causes other than the direct result of rinderpest, such as distomiasis and eating of
poisonous plants while grazing (?). Hence, the total number of deaths on each side
during the period under review was 3 from the Shade group and 7 from the Sun group.
TABLE IX
Summary of results of the immunity test
Group
Shade Group
Sun Group
Serial
number
1
2
3
4
5
6
1
2
3
Baffalo
number
1256
1257
1263
1266
1270
1275
1262
1264
1268
Reaction
No reaction
do.
do.
do.
do.
do.
do.
do.
do.
Result
Immune
„
„
„
„
„
„
„
„
Remarks
..
..
..
..
..
..
..
..
..
Control : Severe reaction.
Died on 10th day due to rinderpest.
152
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III,
Statistical results
TABLE X
Significance of the difference between the means of body weight and blood constituents of
both the groups before vaccination and after reaction period
Constituent
Hb
R. B. C.
C. V. per cent
Ca
P
Mg
Body wt.
Shade group
Significance of the
difference between the
means of the
constituents before
vaccination and
after reaction
period
Difference not significant
Difference not significant
Difference not significant
Difference highly signi-
ficant
Difference just highly
significant
Difference not significant
Difference significant
Remarks
*
*
*
Sun group
Significance of the
difference between the
means of the
constituents before
vaccination and
after reaction
period
Difference highly signi-
ficant
Difference highly signi-
ficant
Difference highly signi-
ficant
Difference Significant
Difference Significant
Difference not significant
Difference highly signi-
ficant
Remarks
*
*
*
*
*
*
* In favour of the group before vaccination
September, 1951]
AMRITAMOY
MUKHERJI
153
(C) Experiment with goat tissue vaccine on goats
TABLE XI
(Showing grouping)
Shade group
Serial
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Total:
Goat
number
116
120
125
126
127
128
135
137
139
142
133
134
119
132
136
Group average
Colour
All black
„ „
„ „
„ „
„ „
„ „
„ „
„ „
„ „
„ „
Black and white
White and red
All white
White with small black
patches on neck
Black with white patch
on abdomen
B. W. (Mean
of 3 conse-
cutive
days)
lb.
35
32
28
36
39
27
32
23
25
29
28
23
28
30
31
458
30
oz.
9
14
13
10
6
2
4
13
9
4
2
13
2
13
5
7
9
Hb
(gm. per 100
ml. of
blood)
6.03
6.55
7.45
6.33
5.94
5.24
6.13
6.23
6.33
7.92
8.17
7.52
6.67
7.52
7.92
101.95
6.79
Sun Group
Serial
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Total:
Goat
number
115
117
118
121
122
123
181
140
141
143
124
114
138
129
130
Group average :
Colour
All black
„ „
„ „
„ „
„ „
„ „
„ „
„ „
„ „
„ „
Black and white
White and red
All white
White with small black
patches on neck
Black with white patch on
abdomen
B. W. (Mean
of 3 conse-
cutive
days) lb.
31
20
23
34
27
40
31
37
25
34
32
26
23
33
30
458
30
oz.
5
9
13
6
14
12
13
14
4
9
0
10
2
13
0
12
9
Hb
per cent
(gm. per 100
ml. of blood)
7.24
5.55
7.92
6.97
7.45
7.17
7.52
5.63
7.38
6.03
7.92
4.87
6.18
6.44
5.35
99.62
0.64
5
154
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III.
TABLE XII
Showing the group average of body wt. and blood constituents of both the groups before
vaccination and after reaction period
Group
Shade group
Sun group
Shade group
Sun group
B. W.
(Mean of 3
consecutive
days)
lb. oz.
30—9
30—9
28—5
26—8
Hb (gm.
per 100
ml. of
blood)
R. B. C.
millions
per
c.m.m.
C. V.
per cent
Before vaccination
6.79
6.64
13.11
12.27
30.43
29.24
After reaction period
6.51
5.88
12.41
10.02
29.23
26.89
Mg. per 100 ml. of
serum
Ca
10.1
10.3
10.1
9.9
Inorg
P
6.25
6.76
7.04
4.96
Mg
2.57
2.45
3.21
2.72
VACCINATION
All the 30 goats were inoculated subcutaneously each with 1 c.c. of 1 per cent
saline emulsion of goat tissue vaccine in the afternoon on 2 April, 1947.
TABLE XIII
Air temperature and relative humidity for the period covered in the experiment, from
3 April, to 10 April, 1947
Readings
Air Temperature
°F
Percentage of relative
humidity
Dry and wet bulb thermometer
readings inside the shed at 9 a.m.,
10.30 a.m. and from 12 a.m.
to 4 p.m. at 1 hour intervals
Range
82.5 to 100.0
13 to 36
Mean
94.1
20.6
Dry and wet bulb thermometer
readings near the place for
exposure of the Sun Group
at 10 a.m. and 11 a.m.
Range
90.0 to 98.0
12 to 22
Mean
94.5
17.5
September, 1951]
AMRITAMOY
MUKHERJI
155
Fig. 7. Daily mean of 8 days' body temperature of both the groups after vaccination from
3 April to 10 April 1947
156
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III.
TABLE XIV
Summary of clinical observations during the period of immunization from 3 to 12 April 1947
Group
Shade Group
Sun Group
Serial
num-
ber
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Goat
number
116
120
125
126
127
128
135
137
139
142
133
134
119
132
136
115
117
118
121
122
123
131
140
141
143
124
114
138
129
130
Body
Tempera-
ture ºF.
Highest
at 9 a.m.
during
reaction
period
106.2
107.2
106.4
106.8
106.8
106.2
107.0
107.2
107.0
106.6
107.0
106.0
105.6
106.0
106.6
107.0
103.2
106.4
106.0
106.6
106.2
105.6
106.6
107.0
106.4
106.4
106.4
105.8
107.2
106.2
Inappetence
Number
of
days
feeds
fairly
3
3
2
1
1
1
.. 1
.. .. 1
2
1
.. 2
1
3
3
1
.. 2
..
..
..
..
..
..
..
..
..
Number
of
days
oft.
feed
2
2
2
4
5
4
6
5
4
1
6
3
2
2
3
7
4
5
2
4
5
5
6
1
3
4
3
3
3
2
Intestinal
disturbance
Number
of
days
soft
faces
..
..
..
..
..
..
.. ..
..
..
..
..
..
..
.. ..
..
..
..
..
.. ..
.. ..
.. 1
..
..
..
..
..
Number
of
days
diarr-
hoea
..
..
..
..
.. 3
.. 6
2
1
.. 5
..
.. 1
..
6
3
5
.. 2
4
5
5
.. 3
3
2
.. 1
..
Mouth
lesions
Vesicles
or
ulcers
nil
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
Body weight
Average
initial
B.W.
lb.
35
32
.. 36
39
.. 32
23
..
.. 28
28
..
.. 31
31
20
28
..
.. 40
31
37
..
..
..
..
..
..
..
oz.
9
14
.. 10
6
.. 4
13
..
.. 2
13
..
.. 5
5
9
13
..
.. 12
13
14
..
..
..
..
..
..
..
Average
final
B.W.
lb.
29
29
.. 32„
33
..
30
21
..
.. 25
24
..
.. 29„
28
16
25
..
..
34
26
31
..
..
..
..
..
..
..
oz.
14
8
.. 9
13
.. 8
9
..
.. 10
4
..
.. 9
0
4
14
..
.. 2
4
12
..
..
..
..
..
..
..
Average
Difference
lb.
5
3
.. 4
5
..
1
2
..
.. ..
4
..
.. 1
3
4
2
..
.. 6
5
6
..
..
..
..
..
..
..
oz.
11
6
.. 1
9
.. 12
4
..
.. 8
9
..
.. 12
5
5
15
..
.. l0
9
2
..
..
..
..
..
..
..
Mortality
Recovered
„
Died on 7th day
Recovered
„
Died on 9th day
Recovered
„
Died on 7th day
Died on 5th day
Recovered
„
Died on 7th day
Died on 5th day
Reocvered
Recovered
„
„
Died on 7th day
Died on 7th day
Recovered
„
„ Died on 4th day
Died on 7th day
Died on 8th day
Died on 6th day
Died on 6th day
Died on 7th day
Died on 5th day
Note—From the 11th day and upto the 21st day after vaccination, 2 goats of the shade group,
viz., Nos. 126 and 136, and 3 goats of the Sun group,
viz.,
Nos.115, 131 and 140 died of debility due to the after effects of rinderpest. Therefore for the collection of immune sera ,only 7 goats of the Shade group and 3 goats
of the Sun group were available.
September, 1951]
AMRITAMOY
MUKHERJI
157
Serum test for evaluation of potency
The results are as follows:
Shade group serum:—Gross potency
....
Sun group serum:—Gross potency
....
26.5 per cent.
12 per cent.
Mortality during the convalescent period and upto the immunity test
Mortality upto the 21st day after vaccination, i.e., prior to bleeding for immune
sera, has already been noted above. There was no more mortality among both the
groups after the 21st day. Therefore, only 7 goats of the Shade group and 3 goats
of the Sun group were available for the immunity test.
TABLE XV
Summary of results of the immunity test
Group
Shade
Group
Sun group
Serial
number
1
2
3
4
4
6
7
1
2
3
Goat
number
116
120
127
133
134
135
137
117
118
123
Reaction
No reaction
„
„
„
„
„
„
„
„
„
Result
Immune
„
„
„
„
„
„
„
„
„
Remarks
..
..
..
..
..
..
..
..
..
Control: Severe reaction; recovered.
TABLE XVI
Statistical results
Significance of the difference between the means of body weight and blood consti-
tuents of both the groups before vaccination and after reaction period
Constituents
Hb
R.B.C.
C.V. per cent
Ca
P
Mg
Body wt.
Shade group
Significance of the
difference between the
means of the
constituents before
vaccination and after
reaction period
Difference not significant
Difference not significant
Difference not significant
Difference not significant
Difference not significant
Difference significant
Difference highly signi-
ficant
Remarks
..
..
..
*
*
¶
Sun group
Significance of the
difference between the
means of the
constituents before
vaccination and after
reaction period
Difference significant
Difference highly signi-
ficant
Difference significant
Difference not significant
Difference significant
Difference significant
Difference highly signi-
ficant
Remarks
*
*
*
..
*
¶
*
* In favour of group before reaction period
¶ In favour of group after vaccination
158
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II [Vol. XXI, Part III.
(D) Experiment with goat tissue vaccine on hill-bulls
TABLE XVII
(Showing grouping)
Group
Shade group
Sun group
Serial
number
1
2
3
4
5
6
7
8
Total
1
2
3
4
5
6
7
8
Total
H. B.
number
179
189
209
2
277
281
40
34
Colour
Black
„
„
Red
„
„
„
„
Group average
211
187
283
284
289
279
10
293
Black
„
„
Red
„
„
„
„
Group average
Mean of 3
consecutive
days Body
wt. in lb.
365
324
280
169
214
212
160
184
1,908
238.5
362
320
181
264
216
200
176
186
1,905
238.1
Haemoglobin
(gm. per
100 ml.
of blood)
7.76
8.94
7.45
8.09
6.44
6.50
7.17
6.55
58.90
7.36
8.84
916
7.04
6.73
6.97
6.23
7.04
7.76
59.77
7.47
September, 1951]
AMRITAMOY
MUKHERJI
159
TABLE XVIII
Showing the group average of body weight and blood constituents of both the groups
before vaccination and after reaction period
Group
B. W. (Mean
of 3
consecutive
days in lb.)
Hb. (gm.
per 100
ml. of
blood
R. B. C.
millions
per
c.m.m.
Per cent Ca
(Mg. per 100 ml.
of serum)
Inorg
P
Mg
Before vaccination
Shade group
Sun group
238.5
2381
7.36
7.47
6.53
0.18
33.5
34.6
10.5
10.5
7.30
7.02
2.13
2.02
After reaction period
Shade group
Sun group
229.5
223.5
6.63
7.09
6.36
5.68
28.2
31.1
10.2
10.3
6.07
6.45
1.98
1.65
Vaccination—All the 16 hill-bulls were inoculated subcutaneously each with 1 c.c. of 1 per cent
saline emulsion of goat tissue vaccine in the afternoon on 6 June, 1947
FIG. 8. Daily mean of 8 days' dry and wet bulb thermometer readings inside the shed.
From 7 to 14 June, 1947
160
INFLUENCE
OF SOLAR RADIATION ON IMMUNITY, II
[Vol. XXI, Part III.
FIG. 9. Daily mean of 8 days' dry and wet bulb thermometer readings in the exposed place.
From 7 to 14 June, 1947
FIG. 10. Daily mean of 8 days' body temperature of both the groups after vaccination.
From 7 to 14 June, 1947
September, 1951]
AMRITAMOY
MUKHERJI
161
TABLE XIX
Summary of clinical observations during the period of immnfiization. From 7 to 15
June, 1947
Group
Shade group
Sun group
Serial
num-
ber
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
H. B.
number
179
189
209
2
277
281
40
34
211
187
282
284
289
279
10
293
Body
Tempera-
ture F.
Highest
at 9 a.m.
during
reaction
period
101.4
101.4
165.0
103.0
101.4
101.6
103.6
101.8
101.6
103.4
104.0
104.6
105.6
103.0
106.0
102.8
Inappetance
Number
of
days
feeds
fairly
..
..
4
2
..
..
3
..
..
..
4
2
1
..
2
..
Number
of
days
off
feed
..
..
..
..
..
..
..
..
..
..
..
..
4
..
4
..
Intestibal disturbance
Number
of
days
soft
faeces
..
..
3
..
..
..
2
..
..
..
..
4
..
..
1
..
Number
of
days
diarrhoea
..
..
..
..
..
..
..
..
..
..
..
..
4
..
4
..
Mouth
lesions
Vesicles
or
ulcers
..
..
..
..
..
..
..
..
..
..
..
..
..
..
ulcers
..
Body wt. in lb.
Average
initial
B. W.
365
324
280
169
214
212
160
184
362
320
181
264
216
200
176
186
Average
final
B. W.
369
322
253
150
210
203
147
182
360
292
154
243
172
184
..
160
Mortality
Difference
4
—2
—27
—19
—4
—9
—1.3
—2
—2
—28
—27
—21
—44
—16
Died
—26
Remarks
Non reactor
do.
Satisfactory reactor
do.
Non reactor
do.
Satisfactory reactor
Non reactor
Non reactor
Mild reactor
do.
do.
Severe reactor
Mild reactor
Severe reactor
Mild reactor
162
INFLUENCE OF SOLAR RADIATION ON IMMUNITY, II
[Vol. XXI, Part III,
Serum test for evaluation of potency
All the six hill-bulls, which were protected with 'Sun group serum', reacted
severely and died within 10 days after inoculation, thus proving that the potency
of the 'Sun group serum' was zero. Among the other six hill-bulls which were
protected with 'Shade group serum', 4 died of rinderpest, but the remaining 2
survived, thus proving that the protective power of the 'Shade group serum' was
better than that of the 'Sun group serum'. Inspite of the fact that 2 out of 6 hill-
bulls survived, the gross potency of the 'Shade group serum' was also calculated
as zero in accordance with the rules for the evaluation of potency of immune serum.
TABLE XX
Summary of results of immunity test
Group
Shade group
Sun group
Serial
number
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
H. B.
number
179
189
209
2
277
281
40
34
211
187
283
284
289
279
293
Reaction
High temperature, diar-
rhoea, off feed, mouth
lesions.
Mild temperature, partial
loss of appetite
No reaction .
do.
High temperature, diar-
rhoea, off feed, mouth
lesions
do.
Mild temperature, partial
loss of appetite
do.
High temperature, diar-
rhoea, off feed, mouth
lesions
Mild temperature, soft
faeces, partial loss of
appetite
High temperature, partial
loss of appetite
Mild temperature, soft
faeces, partial loss of
appetite
High temperature, diar-
rhoea, off feed
High temperature, diar
rhoea, off feed, mouth
lesions
do.
Results
Susceptible
Partially
immune
Immune .
do.
Susceptible
do.
Partially
immune
do.
Susceptible
Partially
immune
do.
do.
Susceptible
do.
do.
Remarks
Died
Recovered
do.
do.
Died
do.
Recovered
do.
Died
Recovered
do.
do.
Died
do.
do.