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The 9 grains taken for the vaseline was finely ground in a sterilised agate mortar,
and to it was added 36 grains of sterilised white vaseline. This was also put into another
sterilised glass bottle with a glass stopper.
The 9 grains taken for the lanoline was also finely ground in a sterilised agate
mortar, and to it was added 54 grains of sterilised neutral lanoline. This was also put
into another sterilised glass bottle like the others.
These three bottles were kept together and were not used until December 4th, a fort-
night from the date on which the vaccines in them were made. This time was allowed to
elapse so as to test the preservative properties of the several mixtures. It was now intended
to vaccinate unvaccinated children with them, making three places of inoculation on
each arm.
The contents of the bottle containing the glycerine vaccine was found to have putre-
fied and moulds were growing inside the bottle. The contents of the other two bottles were
quite unaffected and odourless. This result confirmed what was found in Mysore and
Bengal that the glycerine failed to preserve the vaccine.
On the 2nd December a pot of lanoline vaccine had been received from Bangalore
in good condition, it was stated to have been made on the 26th November; as the glycerine
vaccine had already failed, it was determined to compare the effect of the Mysore lano-
line vaccine with the vaseline and lanoline vaccine made by the author on the 21st
November.
The Mysore lanoline vaccine had the advantage of being five days fresher, but the
disadvantage of a warm journey by post.
Seven children were vaccinated in three places on each arm in the shape of a
triangle.
In each case the upper place was vaccinated with vaseline vaccine.
The front place with lanoline vaccine made here.
The back place with Mysore lanoline vaccine.
The children's names and the results found on the 10th December by author and
Surgeon Captain James were as follows :—
1. Ishar, age four years, unvaccinated previously.—All the places have taken
well. Vaseline vaccine best, Ráwalpindi lanoline vaccine next, and
Mysore least.
2. Moula, aged two years, unvaccinated previously.—The two upper vaseline
ones taken. Of the Ráwalpindi lanoline one taken. Of the Mysore one
taken.
3. Rám Dyál, aged two months, unvaccinated previously.—The two upper ones
vaseline very fine, large vesicles. The two front ones good but small.
The two back ones good but small.
4. Allah Ditta, aged five months, unvaccinated previously.—Upper both very
good ; front both fair ; back both fair.
5. Jumi, aged seven months, unvaccinated previously.—Upper
both good. Front
right arm good. Front left arm nil. Posterior on right arm fair.
Posterior on left arm nil.
6. Dropti, aged twelve months, unvaccinated previously.—Upper both good.
Front both good. Posterior both fair.
7. Sundar, aged eight months, unvaccinated previously.—Upper both good. Front
on right arm doubtful. Front on left arm nil. Posterior both nil.
The result of the above experiment was that the vaseline vaccine took in all the
14 places successes cent. per cent, and gave good large vesicles, the lanoline vaccine
made here gave 11 successes for the 14 insertions or 78.5 per cent., the vesicles were
generally good.
The lanoline vaccine from Mysore gave 10 successes for 14 insertions, or 71.4
per cent. This gave an easy victory to vaseline vaccine, but it was thought that it would
be more satisfactory to repeat this experiment.
On the 6th December the experiment was repeated, the result was. as given
below :—
(1) Kishen Devi, aged two years, unvaccinated previously.—Both upper vaseline
taken. Front Ráwalpindi lanoline, both unsuccessful. Posterior Mysore
lanoline, both unsuccessful.