17
REPORT OF THE KING INSTITUTE, GUINDY
investigated. These rabbits had been vaccinated at least two months before the
onset of symptoms, so in these three cases the vaccinia virus was found in the
tissues sixty days after the primary vaccination which is worth recording. The
cause of death was not the vaccinia virus, but probably an infection with B.
mucosus capsulatus which was isolated from the lungs from all cases, and from
the heart blood and other organs in some of the cases. This organism produced
similar lesions and death in other animals after their experimental infection.
( 11) Differentiation of coliform organisms in water. —Dr . T. Sitapati Ayyar.
His method of the classification of lactose fermenters into human, bovine
and soil types has been applied to 974 water samples from all over the Pres
i-dency, and he has correlated the results with the routine chemical and bacterio
-logical analyses. His findings and the claims of the method to be definite and
useful in the separation of these three types form an important and interesting
paper to be published shortly.
( 1 2
) P a t h o g e n s e l e c t i o n c u l t u r e s .—
D r.
T . S i t a p a t i A y y a r .
In the preparation of autogenous vaccines, particularly from sputum, stools
and materials whose bacterial flora is varied, Solis-Cohen's Pathogen Selective
Culture method of using the patient's own blood has been applied as described in
last year's Annual Report. During the year twenty specimens have been sub
-jected to the new method and the autogenous vaccines made from the selected
organisms have proved beneficial. The work will be reported when sufficient
cases have been collected.
( 1 3 )A
g e i n g o f v a c c i n e s .—
M i l i t a r y A s s i s t a n t S u r g e o n F . G . M a r t i n .
Experiments have been done and are being continued to determine the
period during which certain vaccines remain potent while at air temperatures and
in cold store respectively.
( 1 4
) I m p r o v e m e n t s i n m e d i a .—
M i l i t a r y A s s i s t a n t S u r g e o n J . H . T h e o d o r e .
Experiments have been continued on the use of autolysatès of placenta for
the growth of organisms that do not grow freely on ordinary media. The results
of some experiments are as follows in millions per c.c. :—
Growth on various
standard media.
Growth on placental
extra agar.
Staphylococcus . . .
1,700
1,500
M. Melitensis . . .
3 , 0 0 0
4,000
Streptococcus . . .
700
3,000
Gonococcus I . . .
1,073
2,600
Gonococcus III . . .
7 0 0
2,000
Thus, while there was no improvement in the case of hardy organisms like
staphylococcus, there was very definite improvement in the case of the more
delicate organisms.
(15) Widal reactions. —Assistant Surgeon K. V. Venkataraman.
Experiments are in progress on using this reaction against both the somatic
and the flagellar antigens from the typhoid group of organisms.
(F) SUMMARY.
Comparing this year with the last, the work of the Institute while being a
little less on .the bacterial vaccine side, and remaining about the same in the
general clinical section, has increased a little in the vaccine lymph section and
has very much expanded in the Water and Wasserman sub-sections. The staff
have worked well. I should particularly like to express my thanks and those of
the Assistant Directors to Khan Sahib Muhammad Oomar Sahib Bahadur to
whose zeal and great experience in vaccine lymph work we largely owe our
success in manufacture, to Mr. T. N. S. Raghavachari whose hard work has been
of the greatest use to the Institute and to the Water and Sewage Purification.
Committee, and to Dr. J. H. Theodore whose keenness and ability in the clinical
section have been of the greatest value.
THE KING INSTITUTE, GUINDY,
Dated 23 rd July 1930.
3
H. H. KING, LIEUT.-COL., I.M.S.,
Director.