99.8°, nil, e. t. 100°: sixteenth day m. t. 99.6°, nil; and for a few days longer
similar negative results.
(2) A small monkey (Macacus radiatus) with healthy aspect and blood m. t. 101°p
ossibly not all the injected material reached the peritoneal cavity, as the blood
had congulated and it may have been blood-serum chiefly which penetrated
deeply. Second day m. t. 100°; 18 hours after experiment the animal seems
well, no local swelling, but abdomen slightly tender at site of puncture to the
right of the navel; in the first sample of blood taken from a digit 2 monads
were detected very actively moving and otherwise presenting all the aspects
of the hæmatozoon seen the day previously in the rat; they kept near to each
other and were long watched: temperature at 1° P.M. was 100.6°; at 5 P .M.t.102°;
another active organism was seen in a fresh specimen of the blood from another
digit : t. at 9° P.M. 100° : third day m. t. 101°; a specimen of the blood now
taken showed a single active monad : at 1° P.M. t. 100°6; at 5 P.M. t. 102°;
a flagellate organism like that of the rat was again found in the blood, as
before: at 9° P.M. t. 100.2°: fourth day m. t.101.4°; I failed to detect any
parasite ; e. t. 101.6°,; no parasite seen, but much plasmic matter? and many
white cells; at 9° P.M. t. 99 . 6°: fifth day m.t. 100 . 4° nil, e.t. 102°; plasmic
particles small, many: at 9° P.M. t. 100°: sixth day m.t. 100.6° nil., at 1°P
.M. 100.4°, at 5 P.M. t.
101°, nil, at 9° P.M. t.
100°: seventh day m.t.
100.4°,
n i l ,
e . t . 1 0 1 . 6° a n d l a t e r 9 9 . 6°: e i g h t h d a y m . t . 1 0 0 . 2°,n
i l ,
b u t m a n y p l a s m i c
particles, e.t.
101.4° and 99°: ninth day m.t. 100.4° nil. e.t. 101.4° and 99.6°:
tenth day m.t. 100 . 2°, nil, e.t. 102°, nil, at. 9° P.M. t. 100°: eleventh day
m.t. 100.8°, e.t.s. 101.6°and 99.6°: twelfth day m.t. 100.4°, nil, e.t.s. 101.6° and
99.4°: thirteenth day m.t. e.t. 102° nil; at 9°P.M. t. 100°: fourteenth
day m.t. 101° nil, e.t.s, 102° and 99.6°: fifteenth day m.t. 100.4° nil, e.t. s.
101.6° and 99.8°: sixteenth day m.t. 100.4° nil, e.t.s. 101.6° and 100°: seven-
teenth day m.t. 100.4° nil; and subsequently this animal long remained well.
SUMMARY OF EXPERIMENTAL RESULTS:—Of 15 varied trials one furnished decided posi-
tive results, and the chief object I had in view was thus attained. Records are detailed
here for the guidance of future investigators; and the conditions of effective inoculation
being probably complex, some amendments in the methods above used will doubtless
suggest themselves. Culture of the inoculated blood seems a promising means, varied
at discretion. Respecting the successful result in the monkey, to all appearance the
organism newly visible in the blood was identical with those before found in the rat;
and I calculated that if there were no more than 3 ounces of blood in the monkey and only 2
monads in a drop, there might have been about 3,000 of these organisms circulating on
the second day after inoculation, with a probability of the total being really larger. It
did not seem quite certain that a reproduction of the rat's organism had taken place in the
circulation of the monkey; but possibly this was so to a very limited extent. By experi-
ment it is known that on mixing tainted blood with healthy mule's blood, or that of
healthy bullock or goat, the organisms are not at once rendered quiescent, or killed.
(Steel.)
Having now given the data acquired in Bombay, I proceed to compare
them briefly with other information, including the material sent to me by
Mr. Steel from British Burma.