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Horse No. XXIV.—Brown Country-Bred Mare, aged. A short incision was
made in the white skin of the muzzle, a quarter of an inch in length and just through
the cuticle, so as not to cause any bleeding. A drop of blood was taken from
Horse No. XXII in which very numerous hæmatozoa were present, and a small
quantity, a fraction of a minim, of it was immediately placed between the edges
of the incision. No change was observed until the morning of the fourth day,
when some induration was noted at the seat of inoculation and a pink flush, a
third of an inch in width, surrounding it. The induration increased until the
morning of the sixth day, after which it remained unchanged until the eighth
day, when it was found to have diminished in size and a few hæmatozoa were
discovered in the blood of the general circulation. The latent period occupied
seven days. There was continued fever with remissions for the first nine days
followed by a period of six days until death on the 6th July, the fifteenth day
of the disease, during which the temperature only just exceeded the normal limit
of health.
Autopsy.—Emaciation. Emphysema especially at the apices and along the
free borders of the lungs with a few sub-pleural extravasations ; tissues
stained a yellow colour. Pericardium contained two ounces of fluid. Heart,
yellow gelatinous exudation round the base of the organ, dotted over with
ecchymoses. There were a few sub-endocardial extravasations in the ventricles.
Liver, enlarged, a few calcareous bodies encapsuled in the tissues. Stomach, a
patch of congestion on the mucous membrane of the villous portion. Spleen
enlarged, a few sub-capsular ecchymoses. Kidneys.—Both organs found lying in
a bed of yellow gelatinous exudation, pale but healthy in appearance. Intestines
healthy in appearance. Brain membrane pale, veins distended with dark fluid
blood, tissues pale, lateral ventricles distended with fluid. Nasal mucous
membrane intensely congested.
Horse No. XXVI.—Grey Country-Bred Horse, aged. Two hundred cubic
centimetres of blood were removed from the heart of Horse No. XXII. on 22nd
June 1891, one hour and ten minutes post mortem, and were administered by the
mouth to this animal. The blood given contained very numerous hæmatozoa an
hour previous to death. Slight oedema of no importance however occurred on
the inner side of the right thigh on the 6th July, and six days later ecchymoses
were observed on both conjunctival membranes. At the same time the animal
appeared to be losing flesh. Twice daily a careful examination was made in
order to be " au courant" with any symptoms which might appear, externally or
in the blood. From the time of administering the blood until the commence-
ment of November 1891, the temperature did not exceed the normal range ; but
on the evening of the 5th of that month it rose to 39.2°C. and the following
morning fell to 37.6°C., but again became elevated the same evening and remained
so until death on the 9th.
Autopsy.—Emaciation.
Lungs, well marked emphysema. Localized patch
of consolidation in the centre of the right anterior lobe. The lobules divided off
from one another by lemon-coloured gelatinous exudation, pleura thickened.
Heart, healthy. Liver, small. Spleen, normal in size, no petechiæ. Kidneys,
healthy. In this case it would appear that the post mortem blood given by
gastric ingestion failed to reproduce the disease.
Horse No. XXVII.—Brown Country-Bred Gelding, aged. Subcutaneously
inoculated on the 16th July 1891 with 2.0 c.c. of blood taken from the ventricles
of the heart of Horse No. XXX, sixteen hours after death. One cubic centi-
metre was injected on the right side from right ventricle and 1c.c. was inject-
ed on the left side from the left ventricle. After considerable swelling and œdema
had taken place at the seat of inoculation on the left side, it suppurated. The
swelling and oedema on the right side disappeared to a great extent by the 23rd
July, leaving a tense and tender tumor, which maintained these conditions until
the morning of the 27th, when it was found that the tension of the part had
considerably diminished, and the tenderness on manipulation had almost disap-
peared. At the same time the hæmatozoa were numerous in the blood. The latent
period occupied eleven days. Temperature presented an intermittent type
although it never exceeded the normal range in the horse from 37.2°C. to 39.3°C.
Death occurred on the 29 th July.