35
4th day. On the evening of the 5th day it registered 40.6°C., but from this time
until the morning of the 9th day, upon which death took place, the temperature
gradually fell and was 35°C.
Autopsy.—Body fairly nourished, absence of swelling and œdema from the
extremities and under-surface of the abdomen. Thorax. — No fluid in the pleural
cavities and no pleuritic adhesions. Lungs.—Sub-pleural extravasations of blood
invading right organ but more numerous on left. On section—Tissues healthy.
Cavity of the pericardium devoid of fluid. Heart.—Free from sub-pericardial
and sub-endocardial extravasations. Valves healthy. Liver.—Numerous sub-
capsular petechias. On. section—Tissues presented a nutmeg change. Spleen.—
Large, sub-capsular petechiæ. Tissues, firm. Kidneys.—The right was lying
surrounded by jelly-like exudation and both presented numerous dark-coloured
sub-capsular petechiæ involving the cortical portion of the organs. Stomach.—
Villous portion the seat of a Spiroptera tumour 1.5" in diameter. Small Intes-
tines.—Localized extravasations. Large Intestines.—Few small extravasations.
Bladder presented a healthy appearance.
Bull.—Deccani-bred.—This animal was kept under observation for some time,
during which it appeared quite healthy. It was subcutaneously inoculated on
24th March 1891 with 1.75 c.c. of blood, containing numerous Surra hæmatozoa
taken from the left Jugular Vein of Horse No. XIX. On the evening of the
28th March the temperature rose to 39.7°C. and on the following morning
one hæmatozoon was discovered on microscopical examination after searching
through several specimens of blood. On the 30th no hæmatozoa were found in
the blood, but on the 31st and 1st April a few were observed ; from this date
until the 17th of June no more hæmatozoa or their immature forms were seen
although carefully searched for. On June 17th, eighty-five days after the inocu-
lation of the animal, two Guinea-pigs were inoculated subcutaneously with 0.2 c.c.
No. I Guinea-pig developed hæmatozoa in the blood on the 27th, ten days after
inoculation, whilst No. II Guinea-pig did not develop any until the 5th July, or
eighteen days after inoculation. The organisms were present off and on in the
blood of these small animals for a considerable period. No. I finally succumbing
to the disease on the 8th of November 1891 or 144 days after its inoculation,
whilst No. II died on the 12th October .1891, or 117 days after inoculation.
During the interval which elapsed between the 17th June and 3rd September
1891, no hæmatozoa were discovered in the blood of the bull, although several
coverglass specimens of it were examined every day. Consequently two more
Guinea-pigs were inoculated. These were distinguished as No. III and No. IV
and each received 0.2 c.c. of blood from the bull on the 163rd day after its inocu-
lation. Nos. III and IV Guinea-pigs both developed hæmatozoa on the 10th day,
and the organisms remained almost constantly present in their blood until the
day of their death, 24th October and 14th December respectively.
On 13th November 1891, that is, 234 days after the inoculation of the bull,
two Guinea-pigs Nos. V and VI were inoculated with 0.2 c.c. of blood from that
animal, but as no hæmatozoa were found during the next 3'2 days, they were re-
inoculated on the 16th December 1891, 267 days after inoculation of the bull, but
with negative results a second time. Between the latter date, 16th December 1891,
and 14th March 1892, the bull appeared to be enjoying good health and putting
on flesh. On this latter date, that is, 356 days from the time of inoculation, two
new Guinea-pigs Nos. VII and VIII were inoculated as previously with 0.2 c.c. of
blood from the bull, but this experiment was also followed by negative results.
Since 24th March the bull has been in very fine condition, and as it was desirable to
ascertain whether animals of the bovine species are liable to a second attack of
Surra, it was resolved to re-inoculate this animal with a more virulent type of
Surra obtained by passing the hæmatozoon of the rat (Mus decumanus) through
the horse and donkey. This second inoculation was performed on 22nd August
1892, when l*0c.c. of blood drawn from the Internal Jugular Vein of the donkey,
was subcutaneously injected over the left scapular region, the blood at the time con-
taining very numerous and active hæmatozoa. On the 28th of the same month
a peculiar organism was found in the blood, but on that day only, which will be
hereafter described; The animal put on flesh and appeared in robust health. On
21st September two Guinea-pigs were inoculated with blood drawn from the