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No. II.—The latent period occupied nine days. From the evening of the
first day of the disease until that of the fourteenth day, the temperature was
marked by slight exacerbations at night with remissions in the morning ; after-
wards from the latter date until the evening of the eighty-sixth day the fever
was of the continued type from which intermissions were entirely absent. Death
occurred on the 7th of February 1891, the 89th day of the disease.
Autopsy.—Localized emphysema of the lungs. Spleen small. The other
organs presented a healthy appearance.
No. III.—A pregnant doe ; it was subcutaneously inoculated on November
24th 1890, with 0.5 c.c. of blood containing very numerous hæmatozoa
taken from the Jugular Vein of Horse No. IV. Up to the 30th no organisms
were found in the blood ; on that date it gave birth to three young ones, one
being born dead. The blood of the mother and young ones was free from the
hæmatozoon. On the day following parturition, the mother's blood contained
organisms, and continued to do so off and on until the date of death. The milk
of the mother was examined daily, but no organisms were ever discovered in it ;
nevertheless on December 16th the blood of No. 1 young one contained a few
hæmatozoa, but they were never discovered in that of No. II.
The mother presented a temperature indicating continued fever from the
3rd day until the 90th clay of the disease, with intermissions on the 33rd, .45th,
50th, 58th and 90th days. Death occurred on the 1st March 1891, the 91st of
the disease.
Autopsy.—Body emaciated. Lungs.—The apices were the seat of well
marked localized emphysema. The right middle lobe was consolidated. Liver
enlarged. Stomach.—The mucous membrane at the pyloric extremity was con-
gested, no ulceration. Spleen enlarged to three times normal size. The other
organs were healthy. At a later date, 4th February 1891, the two young ones
were inoculated when sixty-seven days old, each with 0.2 c.c. of blood containing
numerous hæmatozoa taken from Horse No. XI.
No. 1.—The latent period occupied six days. Death occurred on 2nd June
1891, the one hundred and eighteenth clay after inoculation.
No. 2.—The latent period occupied six days. Death occurred on 3rd July
189J, one hundred and forty-ninth day after inoculation.
RATS.
Rat (Mus decumanus).—The blood of this animal was submitted to careful
microscopical examination for a period of fourteen days, and as the hæmatozoon
was not observed in it, subcutaneous inoculation was made with 0.2 c.c. of blood
containing very numerous hæmatozoa from the Jugular Vein of Horse No. VII.
On the next day a few organisms were found in the blood, which in the course
of a few days increased to very numerous and swarming. The rat died on the
seventh day after the inoculation, with its blood teeming with hæmatozoa.
Autopsy.—Body well nourished. All the organs with the exception of the
spleen which was much increased in size and dark-red in colour, presented a
healthy appearance.
Three rats (Mus decumanus) were selected, two full grown and one young
one, on account of their blood being free from the hæmatozoon. After two days
the adult animals received 0.1 c.c. of blood containing a few organisms from Horse
No. VII. The small animal was not inoculated, but placed with the two others
to act as a control experiment, and in order to observe whether it would contract
the disease. The period which elapsed between the inoculation and the first
appearance of the hæmatozoon in the blood of the adult animals was two days,
and on the fifth day the organism was found in the blood of the young animal.
The adult animals survived inoculation by eight days, and the young one by
nine days only.
Autopsies.—In all three animals, respectively, the spleen was much enlarged
and presented numerous localized patches of extravasation which had given rise