cclxvi
SUBCUTANEOUS INOCULATION OF SMALL ANIMALS WITH THE
URINE OF A HORSE IN AN ADVANCED STAGE OF ' SURRA.'
These experiments were undertaken with a double object:—
(i). In order to observe the effect, if any, produced by the toxic constituents in the
urine of horses in an advanced stage of Surra, and presenting the hæmatozoon
in large numbers in the blood, at the time when the urine was voided.
(ii). To ascertain in the event of the animal surviving the toxic effects of the injected
fluid, whether any mature or immature forms of the hæmatozoon are excreted
in the urine, and if so, whether they are capable of reproducing the disease,
by the inoculation of it into another animal.
Consequently on October 14th, 1891, a Guinea-pig and a rabbit were selected, and their blood
submitted to microscopical examination previous to the subcutaneous inoculation of -the
fluid. In the case of each animal, the blood was found to be healthy.
14th October.—A Guinea-pig No. 1, full grown, temperature 1-20 P M. 40.4°C., weight
988.0 grammes, was subcutaneously inoculated at 1-45 P.M. with 5 c.c. of urine slightly acid
in re-action, Sp. Gr. 1,014, and containing a few granular casts, and a trace of albumen.
This was a sample of collected urine voided during the previous twenty-four hours by Horse
No. XXXVIII. on the 50th day after inoculation with horse Surra blood. The hæmatozoa
were swarming in the blood of the horse during the period that the urine was being
collected.
This animal was carefully watched from 2 P.M. until 6 P.M., but with the exception of
the respirations being increased in number immediately after the injection of the urine, and
lasting so until 3-10P.M., when the breathing became tranquil, no toxic symptoms presented
themselves. Temperature P.M. 39.8°C.
15th.—Temperature A.M. 39.3°C. At noon, a second subcutaneous injection of 5 c.c. was
made. The urine was slightly acid, Sp. Gr. 1,016, and contained a few granular casts and a
trace of albumen. No symptoms followed. Temperature P.M. 40°C. No hæmatozoa in
the blood.
16th.—
17th
18th
19th
20th
21st
22nd
23rd
24th
25th
26th
27th
Temperature
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A.M.
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39°C. P.M.
39.3°C. „
39.1°C. „
39.2°C. „
39°C. „
38.6°C. „
38.2°C. „
38.5°C. „
38.7°C. „
38.5°C. „
38°C. „
38.3°C. „
39.8°C.
39.7°C.
39.7°C.
39.4°C.
39.5°C.
39.3°C.
39.2°C.
39.4°C.
39.2°C.
39.4°C.
39.1°C.
39.2°C.
Hæmatozoa absent from the blood.
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30th September, 1892.—The animal is still alive and healthy.
RABBIT.—This is a white coloured animal with pink eyes. Weight 2,145 grammes.
14th October, 1891, 1-20 P.M.—Temperature 39.4°C.
2 P.M.—Inoculated subcutaneously with 10 c.c. of urine from the same source as that
used in the previous experiment.
2-10 P.M.—The pulse and respirations are accelerated, and a few minutes later, the
pupils became slightly contracted. These conditions at 2-26 P.M. were more aggravated,
and lasted until 2-40 P.M., when the pupils began to dilate again and the breathing became
less frequent. At 3-35, the respirations had fallen in number per minute, to just one half
of what they were at 2-26. At 4 P.M. the animal appeared to be in the same condition as
observed previous to the inoculation. Temperature P.M. 39°C.
15th October.—Temperature A.M. 39.3°C. At noon, a second subcutaneous injection of
10 c.c. of urine from the same horse was administered with less result than observed after
the previous one. Temperature P.M. 39.6°C. No hæmatozoa in the blood.
16th.
17th
18th
19th
20th
21st
22nd
23rd
24th
25th
26th
27th
—Temperature A.M.
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39.2°C.
39°C.
39.1°C.
39.3°C.
39.2°C.
38.6°C.
38.8 c C.
38.6°C.
39°C.
38.8°C.
39.1°C.
39.2°C.
P.M.
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39.7°C.
39.5°C
39.6°C.
39.5°C.
39.3°C.
39.5°C.
39.5°C.
39.4°C.
39.3°C.
39.5°C.
39.4°C.
39.4°C.
No hæmatozoa in
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the blood.
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30th September, 1892.—The animal is still alive and healthy.