cxx
decided to endeavour to produce the disease in a somewhat similar manner to that in which
it is naturally contracted. With this end in view. small quantities of blood mixed with a
large hulk of water were to he given at short intervals. Accordingly on the 4th May. four
drops of blood swarming with the Surra hæmatozoa. mixed with one gallon of fresh water
were given to the animal to drink. Up to the 9th no change at all had taken place in the
animal's condition ; and on this date. eight drops of blood containing numerous hærnatozoa
were given in the same way. On the 10th and 11th. the animal showed no change. On the
11th six drops of blood containing Surra hæmatozoa were given in a quart of water. On the
12th. a few small petechiæ were noticed on the membranæ nictitantes. Up to the 24th. with
the exception of a slight indisposition on the 18th. which rapidly passed off. no symptoms of
disease were noticed. On the 24th. he received eight drops of blood containing very numerous
hæmatozoa. in two quarts of water ; but no changes of any importance were noticed up to the
6th June; and on this date. the animal received eight drops of blood. containing numerous
hæmatozoa. in water. No change was observed till the 14th June. when thirty minims of
blood containing very numerous hæmatozoa. were administered in three quarts of water.
On the 16th no change having taken place. ten drops of blood swarming with hæmatozoa
were given in water. On the 17th June. twenty-five drops containing no hæmatozoa. but
taken from Horse No. XXIII suffering from Surra were given in a gallon of water. From
this date up to the 28th June. no change was noticed. On the latter date two hundred minims
of blood containing very numerous hæmatozoa. were given in a bucket of water. From this
date (June 28th) up to the 1st September. absolutely no symptom of disease was noticed;
but from this date until the 6th September. there was a very slight disturbance of tempera-
ture. which. however. remained normal. On the 11th September the temperature had risen
to 39.4°C. in the morning. and numerous Surra hæmatozoa were observed in the blood;
the temperature at noon had risen to 40.4°C. and ranged for the next few days between
38.3° C. and 41° C. during which period the hæmatozoa were swarming in the blood.. For
the next three days. the 16th. 17th. 18th. the temperature varied between 38.3° C. and 39.6° C.
and during this period the hæmatozoa were absent. On the 19th. the temperature was normal.
but a slight eruption appeared on the skin of the fore and hind quarters. and the hind limbs
became swollen and œdematous; a few hæmatozoa were during this time found in the blood.
On the 20th September. although the temperature was only very slightly above normal.
the hæmatozoa were found to be very numerous in the blood. On the 21st. the temperature
became elevated at mid-day and in the evening reached 41°C. and the hæmatozoa were
swarming in the blood. On the 22nd. the temperature had fallen to 37.7°C. that is 3.30°C..and
the hæmatozoa were numerous. On the 23rd the temperature had fallen to normal. and the
hæmatozoa were absent. On the 24th the temperature still remained normal. but a few
hæmatozoa were found in the blood. On the 25th. the temperature was still normal. but
the hæmatozoa were numerous in the blood. On the 26th. the temperature had risen to
40.4°C. in the morning and to 41°C. at 1 P.M.. the hæmatozoa being very numerous in the
blood. The temperature fell from 41°C. to 39.1°C. in the evening but rose again on the
27th to 40.4°C. and reached 42.1°C. at mid-day. the hæmatozoa being very numerous in the
blood. On the 28th. the temperature had fallen from 42.l°C. to 38.5°C. and the hæmatozoa
were absent from the blood; and this condition continued for the next two days. On October
1st. the temperature remained within normal limits. and a few hæmatozoa were found in the
blood. On the 2nd the temperature rose to 39.8°C. and the hæmatozoa were numerous in
the blood. On the 3rd. the temperature at midday reached 41° C. but had fallen to 39° 0.
in the evening. but the hæmatozoa were very numerous in the blood. The morning tempera-
ture on the 4th was 41.1°C. but there was a very considerable fall to 37.4°C. in the evening.
the hæmatozoa remaining very numerous. On the 5th. the morning temperature was normal.
but it rose to 40°C. at midday and the hæmatozoa were numerous in the blood. On the
morning of the 6th and during the day. the temperature was normal and the hæmatozoa few
in the blood. On the 7th the temperature was normal and the hæmatozoa absent. On the
8th. the highest temperature was 39.6°C. but the hæmatozoa were very numerous in the
blood. For the three days. viz.. 9th. 10th and 11th October. the temperature oscillated
between 38.1°C. and 40.1.°C. and the hæmatozoa remained swarming in the blood. On the
12th. 13th and 14th the temperature oscillated between 38.4°C. and 40.6°C.. and during
this time. the hæmatozoa were few in the blood. From the 15th to the 18th. the hæmatozoa
swarmed in the blood. and the highest temperature was 40.l°C; this. during the next few
days. gradually declined and reached 37.6°C. on the 19th. on which day the hæmatozoa were
numerous. On the 20th. the hæmatozoa were few in the blood and the highest temperature
39.1°C. On the following day (21st October). the hæmatozoa were numerous and the
temperature rose to 40°C. and later. before death took place. to 40.3°C.
The points of interest in this case are as follows:—
(i). That the disease was conveyed to an animal by mixing small quantities of blood
containing hæmatozoa. in a relatively enormous bulk of water.
(ii). The very long period of time which elapsed between the last exhibition of blood
containing the hæmatozoon. and the appearance of it in the blood. viz.. 75 days.
This is another instance showing the absolute necessity which exists for observa-
tions on inoculated animals being continued for a very considerable time. before
pronouncing on the efficacy or otherwise of the experiments. It is also of extreme
importance clinically showing as it does the difficulty which exists in fixing the time