ccxxiii
mens were examined every day. Consequently two more Guinea-pigs were inoculated.
These were distinguished as Nos. III and IV and each received 0.2c.c. of blood drawn from
the ear of the Bull, with antiseptic precautions, on the 163rd day after the inoculation of
the animal with Surra. No. III developed Surra hæmatozoa in its blood on the tenth day
following inoculation, and these were present with few exceptions until the day of its death,
October 24th, 1891, fifty-one days after inoculation. No. IV developed Surra hæmatozoa
also on the tenth day after inoculation, and they remained almost constantly present in the
blood until the day of death on the 14th December 1891, one hundred and two days after
inoculation.
During the period which elapsed between September 3rd, on which Guinea-pigs III
and IV were inoculated, and November 13th, 1891, the morning and evening temperatures
of the Bull oscillated as much as two or more degrees ; no hæmatozoa were discovered,
although, as before stated, great care was bestowed upon the examination of the blood
each day.
On November 13th, 1891, that is, 234 days after the inoculation of the Bull, two Guinea-
pigs V and VI were inoculated with 0.2c.c. of blood from the Bulls' ear, but as no hæma-
toza were found during the next 32 days, they were re-inoculated on the 16th December
1891, 267 days after the inoculation of the Bull, but with negative results a second time.
Between the latter date, December 16th, 1891, and March 14th, 1892, the Bull appeared
to be enjoying good health and putting on flesh. On this date March 14th, 1892, that is,
356 days from the time of inoculation, two new Guinea-pigs VII and VIII were inoculated
as previously described with 0.2c.c. of blood from the ear of the Bull, but these experiments
were followed by negative results. Since March 14th, the Bull has been in very fine con-
dition, and as it is desirable to ascertain whether animals of the bovine species are liable
to a second attack of Surra, it was resolved to re-inoculate this animal with a more virulent
type of Surra obtained by passing the hæmatozoon of the rat (Mus decumanus) through
the horse and donkey. This second inoculation was performed on August 22nd, 1892,
at 11 A.M., 1 c.c. of blood drawn from the Jugular Vein of the donkey being injected
subcutaneously over the left shoulder, the blood at the time containing very numerous and
active hæmatozoa.
TABLE.
Showing the dates of inoculation and re-inoculation of the Bull, and also those of the Guinea-Pigs
inoculated with the blood of the former, as well as the result in each case.
Animal.
Bull ..
Guinea-Pig No.
I
„
„
„
„
„
„
„
„
„
II
III
IV
V
VI
VII
VIII
IX
X
..
..
..
..
..
..
..
..
..
..
Inoculat-
ed from
horse
No. XIX.
D.
24
m.
3
..
..
..
..
..
..
..
..
..
..
y.
91
Date of
inocula-
tion of
Guinea-
pigs with
Bull's
blood. D.
17
17
3
3
13
13
14
14
..
..
m.
..
6
6
9
9
11
11
3
3
y.
91
91
91
91
91
91
92
92
Guinea-
Pigs in-
oculated
No. of
days
after
Bull's
inocula-
tion.
Days.
..
85
85
163
163
234
234
356
356
..
..
Date of Hæmato-
zoa 1st
appear-
ance in
the blood.
D.
29
27
5
12
12
m.
3
6
7
9
9
..
..
..
..
..
..
y.
91
91
91
91
91
Guinea-
Pigs died.
D.
8
12
24
14
m.
..
11
10
10
12
..
..
..
..
..
..
y.
91
91
91
91
No. of
days
Guinea-
Pigs
lived.
Days.
..
144
117
51
102
..
..
..
..
..
..
Date of
re-inocu-
lation
with
blood of
Donkey.
D.
22
m.
8
..
..
..
..
..
..
..
..
..
..
y.
92
Date of
re-inocu-
lation of
Guinea-
Pigs ater
inocula-
tion of
Bull.
D.
16
16
m.
..
..
..
..
..
12
12
..
..
..
..
y.
91
91
No. of
days
Guinea-
Pigs in-
oculated
after 1st
inocula-
tion of
Bull.
Days.
..
..
..
..
..
267
267
..
..
..
..
Date of
inocula-
tion of
Guinea-
Pigs after
Bull's re-
inocula-
tion.
D.
21
21
m.
..
..
..
..
..
..
..
..
..
9
9
y.
92
92
No. of
days
Guinea-
Pig in-
oculated
after
re-in-
ocula-
tion of
Bull.
Days.
..
..
..
..
..
..
..
..
..
30
30
Remarks.
{ 1st Positive.
2nd Negative.
..
..
..
..
Results negative.
„
„
„
„
„
The chief points to draw attention to in this case are :—
This is the first case recorded of any animal of the bovine species having been success-
fully inoculated with the hæmatozoon of Surra.
The short period during which the hæmatozoa were observed in the blood of the
animal, and the small numbers of them present at any time during that period.
That although no hæmatozoa or their immature forms were recognizable in the blood
after very numerous and careful observations, nevertheless Guinea-pigs inoculated with
relatively small quantities of it, on the 85th day after the inoculation of the Bull, developed
hæmatozoa exactly resembling the organisms observed in the blood of the animal from
which it was primarily taken, and died from the effects of Surra after a period of 117 and
144 days respectively.
That Guinea-pigs subcutaneously injected with a small quantity of this Bull's blood
on the 163rd day after its inoculation, under exactly the same conditions as those before