124
B received 6.0c.c. serum, survived twenty-two days.
0 received 9.0c.c. serum, survived forty days.
The control animal survived forty-seven days.
In the case of the Guinea-pigs—
X received 6.0c.c. serum, and survived the first appearance of the hæmato¬
zoon after inoculation with the soiled Surra blood, thirty-four days.
Y received 6.0c.c. serum, and survived sixty-six days.
The control animal survived the first appearance of the hæmatozoon in the
blood, eighty-four days.
TABLE XIX.
Animals.
Rabbit A ... ...
B ... ...
C ... ...
Control ... ...
Guiuea-pig X ...
Y ...
„
Control ...
Date of sub-
cutaneous
injections.
...
...
...
...
...
...
...
Quantity of
horse serum
injected.
3.0c.c.
6.0c.c.
9.0c.c.
...
...
...
...
Date of
inoculation
with blood
containing
the hæma¬
tozoa.
...
...
...
...
...
...
...
Quantity of
blood ino-
culated.
0.2c.c.
0.2c.c.
0.2c.c.
0.2c.c.
0.2c.c.
0.2c.c.
0.2c.c.
Date of the
first appear-
ance of'the
hæmatozoa
in blood.
D. m. y.
18-12-90
18-12-90
18-12-90
...
20-12-90
20-12-90
...
Date of
death.
D. m. y.
10-1-91
8-1-91
26-1-91
...
22-1-91
23-2-91
...
Number of
days elaps-
ing be-
tween ino-
culation
and death.
...
...
...
...
...
...
...
In the second series, blood was withdrawn from a horse immediately after
death, in which the hæmatozoa of Surra bad been swarming for some days, and
immediately subjected to the method described by Ogata, as the one he used in
isolating from the blood of dogs and fowls, substances capable of conferring im-
munity against anthrax, and swine erysipelas. Three Guinea-pigs were subjected
to daily injection of this fluid in varying quantities: No. 1 received 0.5c.d., No. 2
0.75 c.c., No. 3 1.0 c.c. daily, for a period of eleven days. After each animal had
received eleven injections, seven days were allowed to elapse, and on the eighth,
all were inoculated with an equal quantity, viz., 0.2c.c. of blood containing
numerous hæmatozoa, taken from a horse the subject of naturally contract-
ed ' Surra'; at the same time a healthy animal was inoculated with a similar
quantity to act as a control experiment.
The results were as follows :—
No. 1 received 5.5o.o. of the solution, and was inoculated with soiled blood
on the 3rd Oct. The hæmatozoon appeared on the 7th October, and death
occurred on the 12th December, sixty-six days after the discovery of the
organism in the blood.
No. 2 received 8.5c.c. of the solution, and was inoculated with soiled blood
on the 3rd October, the hæmatozoon appeared on the 6th, and death occurred
on the 25th October, nineteen days after the discovery of the organism in the
blood.
No. 3 received 11.0 c.c. of the solution, was inoculated with soiled blood on
the 3rd October, the hæmatozoon appeared on the 7th, and death occurred on
the 1st November, twenty-five days after the discovery of the organism in
the blood.
The control animal was inoculated on the 3rd October, the hæmatozoon
appeared on the 8th October, and death occurred forty-two days after the organism
appeared in the blood.
Consequently it will be observed, that the latent period in the three injected
animals occupied 4, 3 and 4 days respectively, whilst in the control animal it
occupied 5 days. The animal which received the smallest quantity of the above
solution lived 66 days, and survived the other two by 47 and 41 days respectively,
and the control by 24 days.