NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
xii
THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY
C
Calcium supplement on the digestion of organic nutrients in fodders in
which lime content is either low or has a tendency of poor assimilation,
the effect of
.
.
.
.
.
.
.
.
.
.
Carotene in green fodder, study on the vitamin A activity of . . .
Case of brucellosis (Aboratus fever) . . . . . . .
Cattle food position in Delhi Province . . . . . .
—
in India, an estimate of the food requirements of milch and other .
—
in India and suggestions as to how present agricultural practice can
be adjusted to provide them, an estimate of the food requirements of
milch and other
.
.
.
.
.
.
.
.
.
.
—,
investigations on famine rations-entrails as a protein substitute
for
.
.
.
.
.
.
.
.
.
.
.
—
feed, the value of boro rice straw as a . . . . . .
—
vitamin C content of the milk of some important breeds of Indian
Coliform organisms in milk, a comparative study in the use of different
media for the estimation of
.
.
.
.
.
.
.
.
Colouring vanaspati with ratanjot to prevent its use as an adulterant in ghee
possibility of
.
.
.
.
.
.
.
.
.
.
Comparative study in the use of different media for the estimation of coli-
form organisms in milk
.
.
.
.
.
.
.
.
.
.
Component of fatty acids of butterfats, hydrogenated groundnut oils and
mustard oil
.
.
.
.
.
.
.
.
.
.
.
Composition of free fatty acids in high-acid ghee . . . . .
Conjunctivitis of sheep and goats in India . . . . . .
Cows and buffaloes, nutritive value of raw and boiled milk of . . .
— of Tharparkar breed, effect of four times milking and handling on the
yield of milk in
.
.
.
.
.
.
.
.
.
.
Cows' udder, effect of local injections of penicillin on staphylococci in the
D
Dairy animals, the preparturient milking of . . . . . .
—
herds in Bengal, incidence of brucellosis in . . . . . .
—
utensils, resazurin test for assessing the sterility of . . .
Detection of adulteration of milk with powder . . . . .
Delhi Province, cattle food position in . . . . . . .
Different oils and fats, the relative rate of absorption of . . . .
Digestibility (some trials on Indian feeding stuffs, XIII, Desi sugarcane
(Katha)
.
.
.
.
.
.
.
.
.
.
PAGE
85
139
215
45
29
23
189
89
185
231
171
231
177
95
69
113
75
269
52
217
225
119
45
221
261
INDEX TO SUBJECTS
xiii
D–contd.
Digestion of organic nutrients in fodders in which lime content is either
low or has tendency of poor assimilation, the effect of calcium supple-
ment on the
.
.
.
.
.
.
.
.
.
.
Disease (Ranikhet) of fowls, transmission of . . . . . .
Dogs in health and when infected with Babesia gibsoni, studies on the
haematology
.
.
.
.
.
.
.
.
.
.
.
Ducklings, pasteurellosis in
.
.
.
.
.
.
.
.
.
.
Ducks, an outbreak of pasteurellosis in . . . . . .
E
Effect of calcium supplement on the digestion of organic nutrients in
fodders in which lime contents is either low or has tendency of poor
assimilation . . . . . . . . . .
—
four times milking and handling on the yield of milk in cows of
Tharparkar breed . . . . . . . . .
—
local injections of penicillin on staphylococci in the cows' udder
Eggs, shell sealing of
.
.
.
.
.
.
.
.
.
.
England (Scotland) and Wales, the herds of 1945-46
.
.
.
.
Entrails as a protein substitute for cattle, investigations on famine rations
Estimate of the food requirements of milch and other cattle in India .
Estimation of coliform organisms in milk, a comparative study in the use
of different media for
.
.
.
.
.
.
.
.
.
Evaporated baffalo milk, investigations on . . . . . .
Experiments on the transmission of surra by the tick, Ornithodorus tholo
zani Laboulbene and Megnin
.
.
.
.
.
.
.
.
Extension in wool fibre, a new method for the study of . . . .
F
Famine rations-entrails as a protein substitute for cattle investigations on
Fatty (free) acids in high acid ghee, composition of . . . . .
—
acids of butterfats, hydrogenated groundnut oils and mustard oil,
content of
.
.
.
.
.
.
.
.
.
.
Fats, the relative rate of absorption of different oil and
.
.
.
.
Feeding (Indian) stuffs some digestibility trials on. XIII, Desi sugarcane
(katha) . . . . . . . . . . .
Feeds and fodders and the requirements of the livestock in the United
Provinces, a survey of the . . . . . . . .
PAGE
85
167
135
249
251
85
75
269
265
275
189
29
231
105
165
209
189
95
177
221
261
34
xiv THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY
F—contd.
Fibre (wool), a new method for the study of extension in . . .
Fodder (green), study on the vitamin A activity of carotene . . .
Fodders and the requirements of the livestock in the United Provinces, a
survey of the feeds and
.
.
.
.
.
.
.
.
—
in which lime content is either low or has tendency of poor assimi-
lation, the effect of calcium supplement on the digestion of organic
nutrients in . . . . . . . . . .
Food (cattle) position in Delhi Province . . . . . .
— requirements of milch and other cattle in India, an estimate of .
— requirements of milch and other cattle in India and suggestions as
to how present agricultural practice can be adjusted to provide them,
an estimate of the
.
.
.
.
.
.
.
.
.
Foot-and-mouth disease in liberated Italy, Waldmann vaccine for . .
Fowls, transmission of Ranikhet disease of . . . . . .
Fresh buffalo milk, the variation of acidity alcohol test and phosphate
number samples of
.
.
.
.
.
.
.
.
.
G
Geese, pasteurellosis in
.
.
.
.
.
.
.
.
.
Geographical and seasonal incidence of surra in the Punjab . . .
Ghee, composition of free fatty acids in high-acid . . . . .
—, possibility of colouring vanaspati with ratanjot to prevent its use as
an adulterant in
.
.
.
.
.
.
.
.
.
.
— (stored), preservative effect of common salt on the development of
acidity in . . . . . . . . . . .
Goat-pox in Hissar District, an out-break of . . . . . .
Goat-pox vaccine, protective inoculation with dry pigeon-pox and sheep
and
.
.
.
.
.
.
.
.
.
.
.
.
Goats against rinderpest, some observations on the immunization of sheep
and
.
.
.
.
.
.
.
.
.
.
.
—,
conjunctivitis of sheep and
.
.
.
.
.
.
.
.
—
(Indian), an account of two species of lungworms from . . .
Grass (Napier), nutritive values of different parts of . . . .
Green fodder, study on the vitamin A activity of carotene in . . .
Groundnut oils (hydrogenated) and mustard oil, component of fatty acids
of butter fats
.
.
.
.
.
.
.
.
.
.
PAGE
209
139
34
85
45
29
23
145
167
237
247
201
95
171
59
243
193
11
69
63
253
139
177
INDEX TO SUBJECTS
xv
H
Haemotology of dogs in health and when infected with Babesia gibsoni,
studies on the
.
.
.
.
.
.
.
.
.
.
Handling on the yield of milk in cows of Tharparkar breed, effect of four
times milking and
.
.
.
.
.
.
.
.
.
Health and when infected with Babesia gibsoni, studies on the haematology
of dogs in
.
.
.
.
.
.
.
.
.
.
.
Herds (dairy) in Bengal, incidence of brucellosis in . . . . .
High-acid ghee, composition of free fatty acids in . . . . .
Highly susceptibility animals against rinderpest, the vaccination of .
Hissar district (Punjab), an outbreak of goat-pox in . . . .
Hydrogenated groundnut oils and mustard oil, component of fatty acids
of butter fats . . . . . . . . .
I
Immunization of sheep and goats against rinderpest, some observations
on the
.
.
.
.
.
.
.
.
.
.
.
Incidence of brucellosis in dairy herds in Bengal . . . . .
India, brucellosis in
.
.
.
.
.
.
.
.
.
.
—, an estimate of the food requirements of milch and other cattle in .
—, conjunctivitis of sheep and goats in . . . . . .
Indian cattle, vitamin C content of the milk of some important breeds of
—
feeding stuffs, some digestibility trials on. XIII, Desi sugarcane
(katha)
.
.
.
.
.
.
.
.
.
.
—
goats, an account of two species of Lungworms from . . .
—
species of brucella
.
.
.
.
.
.
.
.
.
Infected (when) with Babesia gibsoni, studies on the Haematology of dogs
in health and
.
.
.
.
.
.
.
.
.
.
Injections (local) of penicillin on staphylococci in the cows' udder, effect of
Inoculation (protective), with dry pigeon-pox and sheep and goat-pox
vaccine
.
.
.
.
.
.
.
.
.
.
.
Investigations on evaporated buffalo milk . . . . . .
—
famine rations—entrails as a protein substitute for cattle
Italy (liberated) Waldmann vaccine for foot-and mouth disease in (Abstract)
J
Jawar (poisonous), (Sorghum vulgare) Pers., studies on the curing of .
PAGE
135
75
135
217
95
1
243
177
11
217
147
29
69
185
261
63
157
135
269
193
105
189
145
101
xvi THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY
K
Katha, desi sugarcane, some digestibility trials on Indian feeding stuffs
L
Livestock in the United Provinces, a survey of the feeds and fodders and
the requirements of
.
.
.
.
.
.
.
.
.
.
Local injections of penicillin on staphylococci in the cows' udder, effect of
Lungworms from Indian goats, an account of two species of . . .
M
Milch and other cattle in India, an estimate of the food requirements of
Milk, a comparative study in the use of different media for the estimation of
coliform organisms in
.
.
.
.
.
.
.
.
.
.
— (buffalo), investigations on evaporated . . . . . .
(buffalo), the variation of acidity, alcohol test and phosphate number
samples of fresh . . . . . . . . . .
— in cows of Tharparkar breed, effect of four times milking and handling
on the yield . . . . . . . . . .
— of some important breeds of Indian cattle, Vitamin C content of .
— with powder, detection of adulteration of . . . . . .
Milking and handling on the yield of milk in cows of Tharparkar breed,
effect of four times . . . . . . . . . . .
—
of dairy animals, the preparturient . . . . . .
Milks (raw and boiled) of cows and buffaloes, nutritive value of. . .
Mustard oil, component of fatty acids of butterfats, hydrogenated ground-
nut oils and . . . . . . . . . . .
N
Napier grass, nutritive values of different parts of
.
.
.
.
New method for the study of extension in wool
fibre
.
.
.
.
Nutrients (organic) in fodders in which lime content is either low or has
tendency of poor assimilation, the effect of calcium supplement on
the digestion of . . . . . . . . . .
Nutritive value of raw and boiled milks of cows and buffaloes . . .
—
values of different parts of Napier grass . . . . .
PAGE
621
34
269
63
29
231
105
237
75
185
119
75
52
113
177
253
209
85
113
253
INDEX TO SUBJECTS
xvii
O
Oil and fats, the relative rate of absorption of different . . . .
— (mustard) component of fatty acids of butterfats hydrogenated ground-
nut oils and
.
.
.
.
.
.
.
.
.
.
Organic nutrients in fodders in which lime content is either low or has
tendency of poor assimilation, the effect of calcium supplement on the
digestion of
.
.
.
.
.
.
.
.
.
.
.
Organisms (coliform) in milk, a comparative study in the use of different
media for the estimation of
.
.
.
.
.
.
.
.
Ornithodoros tholozani laboulbene and Megnin, experiments on the trans-
mission of surra by the tick
.
.
.
.
.
.
.
.
Outbreak of goat-pox in Hissar district (Punjab) . . . . .
—
of pasteurellosis in ducks
.
.
.
.
.
.
.
P
Pasteurellosis in ducklings
.
.
.
.
.
.
.
.
.
—
in ducks, an outbreak of
.
.
.
.
.
.
.
—
in geese . . . . . . . . .
Penicillin in Veterinary practice, a preliminary review of the use of (Review)
—
on staphylococci in the cows' udder, effect of local injections of
Phosphate number samples of fresh buffalo milk, the variation of acidity
alcohol test and . . . . . . . . . .
Pigeon-pox (dry) and sheep and goat-pox vaccine, protective inoculation
with
.
.
.
.
.
.
.
.
.
.
.
Possibility of colouring vanaspati with ratanjot to prevent its use as an
adulterant in ghee
.
.
.
.
.
.
.
.
.
.
Powder, detection of adulteration of milk with . . . . .
Preparturient milking of dairy animals
.
.
.
.
.
.
.
Preservative effect of common salt on the development of acidity in stored
ghee
.
.
.
.
.
.
.
.
.
.
.
Protective inoculation with dry pigeon-pox and sheep and goat-pox vaccine
Protein substitute for cattle, entrails as a—Investigations on famine
ratiors
.
.
.
.
.
.
.
.
.
.
Punjab, geographical and seasonal incidence of surra in . . . .
R
Ranikhet disease of fowls, transmission of . . . . . .
Ratanjot to prevent its use as an adulterant in ghee, possibility of colouring
vanaspati with
.
.
.
.
.
.
.
.
.
.
PAGE
221
177
85
231
165
243
251
249
251
247
215
269
237
193
171
119
52
59
193
189
201
167
171
xviii THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY
R—contd.
Relative rate of absorption of different oils and fats . . . .
Resazurin test for assessing the sterility of dairy utensils . . .
Rice straw (Boro) as a cattle feed, the value of . . . . .
Rinderpest, some observations on the immunization of sheep and goats
against
.
.
.
.
.
.
.
.
.
.
.
—,
the vaccination of highly susceptible animals against . .
S
Salt (common) on the development of acidity in stored ghee, preservative
effect of
.
.
.
.
.
.
.
.
.
.
.
Samples (phosphate number) of fresh buffalo milk the variation of acidity,
alcohol test and
.
.
.
.
.
.
.
.
.
.
Scotland, England and Wales, 1945-46, the herds of . . . .
Seasonal incidence of surra in the Punjab, geographical and . . .
Sheep and goat-pox vaccine, protective inoculation with dry pigeon-pox
and
.
.
.
.
.
.
.
.
.
.
.
.
—
and goats in India, conjunctivitis of . . . . . .
—
and goats against rinderpest, some observations on the immuniza-
tion of
.
.
.
.
.
.
.
.
.
.
.
Shell sealing of eggs
.
.
.
.
.
.
.
.
.
.
Silage making, suitability of sugarcane tops for
.
.
.
.
.
Some digestibility trials on Indian feeding stuffs. XIII, Desi sugarcane
(katha)
.
.
.
.
.
.
.
.
.
.
.
— observations on the immunization of sheep and goats against rinder-
pest
.
.
.
.
.
.
.
.
.
.
.
Sorghum vulgare Pers., studies on the curing of . . . . .
Species (Indian) of brucella
.
.
.
.
.
.
.
.
.
Staphylococci in the cows udder, effect of local injections of penicillin on
Sterility of dairy-utensils, resazurin test for assessing the . . .
Studies on the curing of poisonous jawar (Sorghum vulgare) Pers. . .
—
on the ha matology of dogs in health and when infected with
Babesia gibsoni
.
.
.
.
.
.
.
.
.
.
Study (comparative) in the use of different media for the estimation of
coliform organisms in milk
.
.
.
.
.
.
.
.
—
of extension in wool fibre, a new method for
.
.
.
.
—
on the vitamin A activity of carotene in green fodder . . .
Sugarcane (desi) (katha), some digestibility trials on Indian feeding stuffs
—
tops for silage making, suitability of . . . . .
PAGE
221
225
89
11
1
59
237
275
201
193
69
11
265
48
261
11
101
157
269
225
101
135
231
209
139
261
48
INDEX TO SUBJECTS
xix
S—contd.
Surra by the tick Ornithodorus tholozani' Laboulbene and Megnin, experi-
ments on the transmission of
.
.
.
.
.
.
.
— in the Punjab, geographical and seasonal incidence of . . .
Survey of the feeds and fodders and the requirements of the livestock in
the United Provinces
.
.
.
.
.
.
.
.
.
.
T
Test (Resazurin), for assessing the sterility of dairy utensils . . .
Tharparkar breed, effect of four times milking and handling on the yield of
milk in cows of
.
.
.
.
.
.
.
.
.
.
.
Transmission of Ranikhet disease of fowls . . . . . .
—
of surra by the tick, Ornithodorus tholozani Laboulbene and
Megnin, experiments on
.
.
.
.
.
.
.
.
.
Trials on Indian feeding stuffs. XIII, desi sugarcane (katha), some diges-
tibility
.
.
.
.
.
.
.
.
.
.
.
Two species of lungworms from Indian goats, an account of . . .
U
United Provinces, a survey of the feeds and fodders and the requirements
of the livestock in
.
.
.
.
.
.
.
.
.
Use of different media for the estimation of coliform organisms in milk, a
comparative study in
.
.
.
.
.
.
.
.
.
.
—
penicillin in veterinary practice, a preliminary review of (Review)
Utensils (dairy), resazurin test for assessing the sterility of . . .
V
Vaccination of highly susceptible animals against rinderpest . . .
Vaccine, protective inoculation with dry pigeon-pox and sheep and goat-
pox
.
.
.
.
.
.
.
.
.
.
.
—
(Waldmann) for foot and mouth disease in liberated Italy (Abstract)
Value of boro rice straw as a cattle feed
.
.
.
.
.
.
.
Values (nutritive) of different parts of Napier grass
.
.
.
.
Vanaspati with ratanjot to prevent its use as an adulterant in ghee, possi-
bility of colouring
.
.
.
.
.
.
.
.
.
.
.
Variation of acidity, alcohol test and phosphate number samples of fresh
buffalo milk
.
.
.
.
.
.
.
.
.
.
.
Veterinary practice, a preliminary review of the use of penicillin in (Review)
PAGE
165
201
34
225
75
167
165
261
63
34
231
215
225
1
193
145
89
253
171
237
215
XX
THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY
V—contd.
Vitamin A activity of carotene in green fodder, study on . . .
Vitamin C content of the milk of some important breeds of Indian cattle
W
Waldmann vaccine for foot-and-mouth disease in liberated Italy (Abstract)
Wales (Scotland and England), 1945-46 the herds of . . . .
Wool fibre, a new method for the study of extension in . . . .
Y
Yield of milk in cows of Tharparkar breed, effect of four times milking
and handling on . . . . . . . . . .
PAGE
139
185
145
275
209
75
MGIPC—M—25 AR—15-4-52—700.
2
Vaccination Against Rinderpest.
Infected spleens collected at the height of reaction were minced and to this twice the quantity (by
weight) of 60 per cent glycerol was added. This product was then aged either at 37°C. or at 0°C.
Bennett concluded that glycerolized spleen pulp was rendered safe for vaccination purposes by storage
either for three days at 37°C. or for three months at 0°C. Stress was laid on dosage—the fresher
the vaccine the smaller the dose but 1 gram of spleen pulp per 100 kilograms live weight was consi-
dered suitable. This author also stated that vaccine after treatment at 37° C. could be stored f or
many months in the cold and used in the field without loss of antigenic power. Pfaff [1938] has
examined the subject of vaccination with glycerolized virus.
Vaccination of hill-bulls. Our experiments were designed in a similar manner to those of Bennett.
Line E virus was employed to infect bulls and spleens were collected in every case on the fifth day,
Twice the weight of 60 per cent sterilized glycerol was added to the minced tissue. The mixture
was stored at 37°C. and inoculations were made from it at various intervals. Table I shows t he
results of experiments on bulls, weighing about 100 kilograms.
TABLE I
Glycerolized vaccine (line E) in hill-bulls
Period of storage
37° C. for 24 hours
.
.
.
.
37°C. for 48 hours
.
.
.
.
37°C. for 72 hours . . . .
37°C. for 72 hours followed by storage
in refrigerator for 7 days
for 14 days . .
for 21 days . .
No. of
Animals
1
1
1
1
1
1
1
1
2
2
Dose of vaccine
suspension
3.0 c.c.
do.
do.
do.
do.
do.
do.
do.
do.
do.
Reaction
nil
do.
do.
do.
do.
do.
do.
Reacted and died
of rinderpest ;
day 11*
nil
do.
Tested after
days
17
17
16
16
15
15
27
20
13
Result of
test
I
Slt R; S
R; D
Slt R; S
R; S
I
R; D
I
R; D
Sit R=Slight temperature reaction ; R= reaction ; I=Immune; D=Died ; S=Survived ;
*Possibly an accidental infection
It would appear from Table I that the vaccine was rendered safe for use after only 24 hour' s
storage at 37°C., but with the dosage employed it failed to impart complete or consistent immunity;
also that the vaccine lost its potency completely after three weeks in cold storage.
To elucidate further the viability of the virus used for preparing the vaccine, spleen tissue was
emulsified in double its weight of distilled water, stored at 37°C. in flat containers and tested at the
same dose rate at 24, 48 and 72 hours. One of the two bulls tested with material stored for 24 hours
developed rinderpest and died on the tenth day. Its companion showed no reaction but developed
rinderpest on immunity test. All the remaining animals reacted on test with virulent virus. Thi s
experiment indicated that rinderpest virus in the form of thick suspensions is very variable in potency
when stored at 37°C.
In view of the fact that, line E virus was of highly infectious nature and therefore not likely
to be safe for widespread field use, further work was prosecuted with the less virulent virus, line D.
Vaccine was prepared in exactly the same way from line D, except that the period of ageing at 37°C.
was kept at two days. The results are shown in Table II.
J. R. HADDOW AND J. A. IDNANI
3
TABLE II
Glycerolized vaccine (line D) in hill-bulls
Period of storage
37°C. for 48 hours
.
.
.
.
37°C. for 48 hours, followed by storage
in refrigerator
for 7 days . . .
for 14 days . .
for 21 days . .
for 28 days . . .
No. of
animals
1
1
2
1
1
1
1
2
2
1
1
2
1
1
2
Dose of vaccine
suspension
3.0 c.c.
do.
30.0 c.c.
3.0 c.c.
do.
30.0 c.c.
Died of other
causes
3.0 c.c.
30.0 c.c.
3.0 c.c.
do.
30.0 c.c.
3.0 c.c.
do.
30.0 c.c.
Reaction
nil
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
Tested after
days
12
12
12
10
10
10
12
12
14
14
14
14
14
14
Result of
test
Slt R; S
R; D
I
I
R; D
I
I
I
R; S
I
I
R; S
R; D
I
The results indicate that the line D vaccine was safe for use after two days' ageing at 37°C.
and that it retained its antigenic value for four weeks, when administered at the higher dose rate,
which alone gave solid immunity.
The period of ageing of the vaccine was then further reduced to 24 hours in one lot and 30 hours
in another. The result of this experiment is given in Table III.
TABLE III
Glycerolized vaccine (line D) in hill-bulls
Period of storage
37°C. for 24 hours
.
.
.
.
37°C. for 30 hours
....
37°C. for 24 hours, transported 90
miles in 2 days on ice
37°C. for 30 hours transported 90 miles
in 2 days on ice
No. of
animals
1
1
2
2
2
11
11
Dose of vaccine
suspension
3.0 c.c.
do.
30.0 c.c.
3.0 c.c.
30.0 c.c.
3.0 c.c.
30.0 c.c.
Reaction
nil
do.
R; D
nil
do.
Slt R.
Slt R.
Tested after
days
23
23
23
23
2 months
12 months
2 months
12 months
Result of
test
R; D
I
Slt R; S
I
3 tested; I
4 tested ; I
1 tested ; I
8 tested ; I
In this experiment, as well as in previous tests, it became apparent that two important factors
needing control are the period of ageing and the size of the dose. After the vaccine has been rendered
safe, a fairly large dose has to be employed to ensure protection.
Another brew of glycerolized vaccine was now prepared with line D virus and tested after 18,
24 and 48 hours at 37°C. (Table IV).
4
Vaccination Against Rinderpest
TABLE IV
Glycerolized vaccine (line D) on hill-bulls
Period of storage
37°C. for 18 hours
.
.
.
.
37°C. for 24 hours
.
.
.
.
37°C. for 48 hours
.
.
.
.
No. of
animals
2
2
1
1
2
1
1
2
Dose of vaccine
suspension
3.0 c.c.
30.0 c.c.
3.0 c.c.
do.
30.0 c.c.
3.0 c.c.
do.
30.0 c.c.
Reaction
Slt R.
do.
R; D.
Slt R.
do.
nil
do.
do.
Tested after
days
25
25
25
25
24
24
24
Result of
test
I
I
I
I
R; D
Slt R; S
I
The results shown in Table IV confirm the finding that at least 30.0 c.c. of glycerolized vaccine
heated for 48 hours at 37° C. have to be employed to ensure protection.
Vaccination of goats. Vaccines were prepared with line E and line W viruses. A few tests
were also made on hill-bulls.
TABLE V
Glycerolized vaccines in goats (line E) and hill—bulls (lines E and W)
Period of storage
No of
animals
Dose of vaccine
suspension
Reaction
Test after
days
Result of
test
Vaccine prepared with line E virus—test in goats
37° C. for 48 hours
.
.
.
.
37°C. for 48 hours followed by storage
in the refrigerator for 7 days
for 14 clays . .
for 21 days . .
for 28 days . .
for 28 days . .
1
1
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
1
1
1
1
2
1
1
1.5 c.c.
do.
7.0 c.c.
15.0 c.c.
do.
1.5 c.c.
do.
7.0 c.c.
do.
15.0 c.c.
do.
1.5 c.c.
do.
7.0 c.c.
do.
15.0 c.c.
do.
1.5 c.c.
do.
7.0 c.c.
15.0 c.c.
do.
1.5 c.c.
do.
7.0 c.c.
15.0 c.c.
do.
nil
do.
Slt R
nil
Slt R
nil
Slt R
do.
D. other causes
nil
do.
Slt R; D; P
nil
D. other causes
nil
nil
Late R
nil
do.
do.
do.
do.
do.
R. D. other causes
nil
D. P. P
nil
20
20
20
20
20
22
22
22
D; P
22
15
15
15
15
17
17
17
17
17
23
23
23
Slt. R; S
D
I
I
D. other causes
Slt R; S
D
I
1
Slt R; S
I
I
I
D
D; P. P
I
R; S
D. other causes
D; P
I
Slt R; S
Vaccine prepared with line E virus—test in bulls
37°C. for 72 hours
.
.
.
. 1
1
1
3
3.0 c.c.
do.
do.
30.0 c.c.
Died of other
causes
nil
Slt R
nil
20
20
20
D. other causes
do.
I
Vaccine prepared with line W wirus—test in bulls
37°C. for hours 72 . . . .
1
2
3
3.0 c.c.
do.
30.0 c.c.
nil
do.
do.
20
20
20
Slt R; S
I
I
Slt R= Slight temperature reaction ; P=Pneun onia ; PP =Purulent pertonities