H. K.
LALL
13
Goatis produced a very severe reaction in all the goats except Nos. 950, 84 and 104 which had reacted
mildly in the previous experiment. Two goats (Nos. 49, 998) which were inoculated with mixed
serum and goatis in the previous experiment exhibited a severe reaction thus indicating that serum
and goatis mixed had no immunizing value. Goat Nos. 603 and 998 died showing lesions of rinder-
pest. It appears from these observations that goatis need not necessarily cause mortality in all the
injected goats and that it produces a different type of reaction in individual animals. Blood virus
did not produce a reaction in any way different from that produced by goatis.
Four animals reacted on the fourth day, three on the fifth day and one (No. 9) without having
any thermal reaction remained very dull for a considerable period, thus proving that the temperature
was not the only criterion of the severity of the reaction. From this experiment it became clear
that:
(i) Goatis by itself was of no use because of the severe reaction produced by it, and
(ii) serum and goatis mixed were also useless as their inoculation failed to produce any reaction.
Experiment No. 3
Batch No. of the virus
.
.
.
.
.
.
.
.
Date of preparation of virus . . . . . . .
Date of experiment . . . . . . . .
190
8 December, 1944
9 December 1944
Thus, in order to compare the reactions of goatis and serum injected at the same time and goatis
followed by serum 24 hours later another five animals (78, 91, 70, 73 and 100) were injected with
0.01 gm. goatis in 1 c.c. of saline followed by 5 c.c. of anti-rinderpest serum, half an hour later, four
(16, 882, 808, 95) were inoculated with goatis followed by serum the next day and one goat (No. 859)
was kept as a control (goatis alone). The results are tabulated in Table III.
TABLE III
Reaction of Betal goats to goatis followed by serum immediately and 24 hours later
Nos. of goats
859
.
.
.
17 . . .
882
.
.
.
808
.
.
.
95 . . .
100 and 78 . .
70 . . .
91 . . .
73 . . .
Vaccine used
Goatis
.
.
.
.
Goatis followed by 20 c.c. of
serum next day
do. . . .
do. . . .
do. . . .
Goatis followed by 5 c.c. of
serum 30 minutes later
do.
. . .
do.
. . .
do.
. . .
Type of reaction
No thermal reaction other-
wise very dull
Very severe . . .
Fairly severe . . .
do.
.
.
.
.
Mild
.
.
.
.
No reaction (No. 100 got 8
c.c.)
Severe
.
.
.
.
Mild
.
.
.
.
do.
.
.
.
.
Day of reaction
....
5th day . .
4th day . .
do. . .
3rd day . .
....
5th day . .
4th day . .
do. . .
Duration of
reaction
....
5 days, aborted on
6th day
4 days
5 days
do.
....
5 days
3 days
2 days
The control as well as the four goats which got the serum 24 hours after the injection of virus
reacted severely. Four out of five injected with goatis and virus at the same time had a mild reac-
tion and no loss of appetite was noticed in them. No. 17 aborted, while goat No. 859 was fairly
dull without having any thermal reaction. The observations further reveal that serum and goatis
given at the same time produce a mild reaction in Betal goats and in virtue of this some immunity
is likely to be produced.
Experiment No. 4
Batch No. of the virus . . . . . . . . .
Date of preparation of virus
.
.
.
.
.
.
.
Date of experiment . . . . . . . . .
253
13 January, 1945
8 January, 1945