208
Studies on Pleuropneumonia
55ºC. for 2 days, the first test indicated that a strong immunity was conferred by the vaccine. These
results were not repeated in a second test, although the conditions of this test were somewhat different
from the first. A modification of Willem's tail tip vaccination, using slightly attenuated cultures,
produces a high grade immunity superior to that produced by cultures attenuated so as to be safe
on subcutaneous inoculation. An equally good immunity can be produced by subcutaneous ino-
culation of lymph virus refrigerated at 5°C. for just over three months.
7. The direct agglutination test did not give clear-cut reactions in assessing immunity or in
serological identification of strains.
8. The morphological and cultural characters of the organism isolated from cases of bovine
pleuropneumonia in Assam are described.
ACKNOWLEDGEMENTS
The authors gratefully acknowledge the help and courtesy of the following gentlemen who
supplied pathological materials and strains of the bovine pleuropneumonia virus for comparative
study : Mr R. Daubney, C.M.G., Director of Veterinary Services, Kenya; Dr A. W. Turner, Div-
sion of Animal Health, Melbourne, Australia; Dr P. J. du Toit, Director of Veterinary Services
Onderstepoort, Union of S. Africa. Thanks are due to Rai Sahib S. C. Ghose, G.B.V.C., late Super-
intendent, Civil Veterinary Department, Assam, for information concerning contagious bovine pleu-
ropneumonia in his province and to Mr Gopalkrishnan, G.M.V.C.. Veterinary Investigation Officer,
Assam, who helped with clinical work in the field and in the supply of materials, as well as with ex-
perimental vaccination of cattle in Assam. Mr Ram Narain, L.V.P., assisted in much of the bac-
teriological work and in checking references, and Mr J. A. Idnani, G.B.V.C.. also helped in the inves-
tigation into the nature of the abomasal lesions in virus inoculated cattle; to both these officers
on the staff of the Institute the help rendered is gratefully acknowledged.
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