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time of the discovery of the organism in the blood, the temperature registered 37.4°C., but at midday
had attained a much higher degree, 39.8°C., and a maximum of 41.2°C. was reached on the evening of
the second day of the disease. The fever was of the continued type accompanied by frequent remissions
and exacerbations and intermissions at irregular intervals. Death occurred on the fourteenth day of
the disease.
Autopsy.—Body very slightly emaciated, the extremities were swollen and œdematous. Lungs.—
The apices were the seat of localized emphysema. Pericardium.—The cavity contained about one pint
of dark blood-stained fluid. Heart.—The base is encompassed by yellow-coloured exudation and dotted.
over with sub-pericardial petechiæ. On section.—No sub-endocardial extravasations in the right or left
auricles or right ventricle, but there were well marked ones in the left ventricle. Liver enlarged, fairly
healthy. Spleen.—Three times the normal size, numerous sub-capsular petechiæ. Kidneys petechiated.
Stomach.—Cuticular portion stained yellow. Villous portion healthy. Small Intestines .—Limited area
petechiated, no ulceration. Large Intestines healthy. Rectum presents a patch which is partially
denuded of the superficial portion of the mucous membrane. Parasites none.
Rabbit No. II.—This animal was subjected to inoculation on the 21st July 1892, with 0.75 c.c.
of blood, containing numerous hæmatozoa, drawn from the heart of Horse No. LXII, fifteen minutes
post mortem. This horse was inoculated with rat's (Mus decumanus) blood. The latent period
occupied five days. The temperature rose to 41.2°C. on the morning of the first appearance of the
hæmatozoon, and the same evening registered 41.7°C. (107°F.). The animal succumbed on the morn-
ing of the 12th August.
(ii).—Results Negative.—Bull No. I, Rabbit No. I.
Bull No. I.—Deccani-bred. This animal was kept under observation for some time, during which
it appeared quite healthy. It was subcutaneously inoculated on 24th March 1891 with 1.75 c.c. of blood,
containing numerous Surra hæmatozoa, taken from the jugular vein of Horse No. XIX. A consider-
able amount of swelling and œdema, with tenderness on manipulation followed at the seat of inoculation.
On the evening of the 28th May, the temperature rose to 39.7 °C., and on the following morning one
hæmatozoon was discovered on microscopical examination after searching through several specimens of
blood. On the 30th no hæmatozoa were found in the blood, but on the 31st and 1st April a few were
observed; from this date until the 17th June, no more hæmatozoa or their immature forms were seen
although carefully searched for. On June 17th, eighty-five days after the inoculation of the animal,
two Guinea Pigs were inoculated subcutaneously with 0.2 c.c. of blood from the Bull. No. I Guinea Pig
developed hæmatozoon in the blood on the 27th, ten days after inoculation, while No. II Guinea Pig did
not develop any until the 5th July, or eighteen days after inoculation. The organisms were present off
and on in the blood of these animals for a considerable period. No. I finally succumbing to the disease
on the 8th of November 1891 or 144 days after its inoculation, whilst No. II died on the 12th October
1891, or 117 days after inoculation. During the interval which elapsed between the 17th June and
3rd September 1891, no hæmatozoa were discovered in the blood of the bull, although several cover-glass
specimens of it were examined every day. Consequently two more Guinea Pigs were inoculated.
Nos. III and IV each received 0.2 c.c. of blood from the bull on the 163rd day after its inoculation.
These animals both developed hæmatozoa on the 10th day and the organisms remained almost constantly
present in their blood until the day of their death, 24th October and 14th December respectively.
On the 13th November 1891, that is, 234 days after the inoculation of the bull, two Guinea Pigs Nos.
V and VI were inoculated with 0.2 c.c. of blood from that animal, but as no hæmatozoa were found
during the next 32 days, they were re-inoculated on the 16th December 1891, 267 days after the inocu-
lation of the bull, but with negative results a second time. Between the latter date, 16th December
1891 and 14th March 1892, the bull appeared to be enjoying good health and putting on flesh. On
this latter date, that is, 356 days from the time of inoculation, two new Guinea Pigs No. VII and
VIII were inoculated as previously with 0.2 c c. of blood from the bull, but this experiment was also fol-
lowed by negative results. Since 24th March, the bull has been in very fine condition, and as it is
desirable to ascertain whether animals of the bovine species are liable to a second attack of Surra, it was
resolved to re-inoculate this animal with a more virulent type of Surra, obtained by passing the hæma-
tozoon of the rat (Mus decumanus) through the horse and donkey. This second inoculation was performed
on the 22nd August 1892, when 1.0 c.c. of blood drawn from the jugular vien of the donkey, was subcuta-
neously injected over the left scapular region, the blood at the time containing very numerous and
active hæmatozoa. No swelling or œdema followed, only slight subcutaneous thickening in the tract
of the injection needle. On the 28th of the same month a peculiar organism was found in the blood, but
on that day only. The animal put on flesh, and appeared in robust health. On the 21st September
1892 two Guinea Pigs were inoculated with blood drawn from the jugular vein, but up to the 14th
November 1892, their blood, which had been examined daily, failed to show any signs of the Surra hæma-
tozoon. The blood of Bull No. I was submitted to microscopical examination until the 22nd July 1893,
a period of 345 days after the date of the second inoculation; no Surra hæmatozoa were discovered, but
only one giant form of Trypanosoma on 27th August 1892.
Rabbit No. I was subjected to inoculation on the 21st July 1892, with 0.75 c.c. of blood
containing numerous hæmatozoa, drawn from the heart of Horse No. LXII, fifteen minutes post
mortem. This horse was inoculated with rat's (Mus decumanus) blood. It succumbed on the morning
of Aguust 1st, 1892, but no hæmatozoa were discovered in the blood.