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from this date until the midday of the 9th it steadily rose, attaining a maximum of 39.8° C.; after this it
gradually became reduced until on the morning of the 11th it was 36.8°C. and continued oscillating for
the next eight days. After the second inoculation on the 19th November, the temperature attained a
maximum of 40.1°C. at midday on the 21st, reaching a maximum three days later of 36.2°C. After this
date it seldom exceeded the normal limits.
No. XLIV.—Dunn Country-Bred gelding, aged. Inoculated subcutaneously on the 2nd October 1891
with 1.0 c.c. of blood swarming with hæmatozoa taken from the hearts of two rats (Mus decumanus)
immediately after death. Swelling and œdema followed at the seat of inoculation accompanied with
considerable tenderness on manipulation. The blood remained free from hæmatozoon during the whole
period during which the animal was under observation. The temperature, which was 35.6° C. at the time
of the injection of blood gradually became elevated, attaining a maximum of 39.8°C. at midday on the
12th October, and on the following morning registered only 36.2° C.—a fall of 3.6°C. From this date
until death on the 27th November the thermometric readings were very variable, but were marked by
morning remissions and exacerbations which attained a maximum sometimes at midday, but more
frequently in the evening.
Autopsy.—Body emaciated. Lungs.—The
anterior portion of each organ was consolidated.
Heart.—The base of the organ is the seat of cherry-coloured exudation, otherwise there were no
pathological changes to note. Liver small and healthy. Spleen, normal in size, numerous sub-capsular
circular patches. Stomach.—Spiroptera tumour involving the villous portion, otherwise healthy. Kidneys
healthy.
(d) SUBCUTANEOUS INOCULATION AND RE-INOCULATION AT A LATER PERIOD WITH SOILED RAT'S BLOOD
WITH NEGATIVE RESULTS ; FURTHER INOCULATION OF THE SAME ANIMAL WITH SOILED HORSE SURRA
BLOOD. RESULTS POSITIVE.
(i).—Directly into the Jugular vein.
Horse No. XIII.
XIII.— Pony Country-Bred, entire, aged. Directly inoculated into the jugular vein on the 22nd of
January 1891, with 0.6 c.c. of blood containing numerous hæmatozoa taken from the heart and vessels of
a rat (Mus decumanus). As no symptoms supervened the animal was re-inoculated on February 12th
with 1.0 c.c. of blood containing numerous hæmatozoa from a similar source as on the first occasion.
Swelling and œdema, however, followed at the seats of inoculation, but no organisms were discovered in
the blood. After an interval of seventy-six days, the animal was inoculated with 1.0 c.c. of blood from
Horse No. XIX in order to prove whether any protection had been acquired through the previous
injections of rat's blood.
The temperature after the first inoculation varied between a maximum of 38.5°C. and a minimum of
36.6°C., during the second period between 38.6°C. and 36.1°C., and during the latent period of the horse
blood between 38.4°C and 37.4°C. From the first day until the thirty-eighth day of the disease, on which
latter day death occurred, continued fever accompanied by remissions and marked intermissions at
irregular intervals was present, the hæmatozoon being present in the blood on twenty-eight days.
Autopsy.—Body fairly nourished; considerable œdema of the under-surface of the abdomen. Lungs.—
The apices were the seat of localized emphysema. The pericardial sac contained a few ounces of yellow-
coloured fluid. Heart.—A few sub-pericardial ecchymoses; cavities free from sub-endocardial extravasa-
tions. Liver.- Healthy. Spleen.— Normal in size, presented a few sub-capsular petechiæ.
Kidneys.—
Healthy. Stomach— Cuticular portion yellow in colour; villous portion presents a few congested patches
Intestines.— Healthy ; no parasites.
(ii).—Subcutaneously.
Horses Nos. XII, XLVIII, XLVI, LVIII.
Horse No. XII.—Country-Bred
gelding, aged. Subcutaneously inoculated on the 21st January 1891
with 1.0 c.c. of rat's blood containing numerous hæmatozoa taken from the heart and great vessels of a
rat (Mus decumanus) immediately after death. Swelling and œdema accompanied by tension and
tenderness of the part appeared up to the evening of the fifth day, but disappeared entirely in the course
of a few days. As no symptoms of disease had supervened by the 12th February, the animal was re¬
inoculated with 2.0 c.c. of blood of a rat containing hæmatozoa on that date. On the 5th April, as no hæma-
tozoa had been discovered in the blood, the animal was inoculated with one drop of blood containing
hæmatozoa on the muzzle, taken from Horse No. XIX. During the first period lasting twenty-two
days the temperature oscillated between 38.5°C. and 36.8°C., during the second. lasting fifty-two days,
between 38.7°C. and 36.6°C. and during the third after inoculation from the horse between 38.6°C. and
38.1°C. The latent period occupied six days. There was continued fever from the first until the even-
ing of the tenth day of the disease, the maximum temperature 40.9°C. being attained on the evening of
the sixth day, and it is a point of great interest that although the hæmatozoon was absent during the
8th, 9th and 10th days, the temperature nevertheless remained elevated. On the morning of the 11th,
the thermometer registered 2.1°C. lower than on the previous night; the organisms, however, had re-
appeared and were noted as few in the blood. Another rise in temperature took place on the twelfth
and remained high until the evening before death which took place on the sixteenth day of the disease.