145
Autopsy.—Body somewhat emaciated, subcutaneous tissues bright yellow colour.
Thorax.—Pleural cavities each contain about two pints of a reddish coloured fluid, feebly alkaline
in reaction.
Lungs.—Surfaces petechiated.
Heart.—8 lbs. 14½ ozs., considerably hypertrophied, orange-coloured gelatinous material surrounding
base of the organ. On section several small subendocardial extravasations.
Abdominal Cavity.—Contains two pints of a pale coloured fluid.
Liver.—13 lbs. 15½ ozs., anterior-surface of right lobe presents numerous small concretions. On
section tissues firm. Spleen.—4 lbs. 1½ ozs., much enlarged. Both surfaces dotted over with numerous
small purple and bright red coloured petechiæ. On section tissues dark in colour, soft and flabby.
Kidneys.—Right 1 lb. 14 ozs., Left 1 lb. 13 ozs., capsules strip easily. Few petechiæ dotted over the
external surfaces of the organs. On section tissues pale. Stomach, on section, contains semi-digested food.
Cuticular portion presents four spiroptera tumours, villous portion, healthy. Intestines healthy. Bladder
ditto, contains no urine.
In this case the temperature, pulse, respiration, water drunk, urine passed and total urea excreted
were noted.
Two Guinea Pigs were inoculated on July 25th, 1893, each with 0 25 c.c. of blood, containing very
numerous hæmatozoa, taken from the heart of Dog No. VI, one hour post mortem
No. I Guinea Pig.—The latent period occupied 8½ days. On the evening before the appearance
of the hæmatozoon in the blood, temperature rose to 40°C. and on the second evening of the disease
registered 40.4°C., but never again attained the same altitude. The animal lived 18 days after the
appearance of the hæmatozoon in the circulation. There were two paroxysms lasting 4 and 10 days
respectively, and one intermission lasting 4 days.
No. II Guinea Pig.—The latent period occupied 10½ days. The temperature only exceeded 40° C.
on one occasion during the whole period the case lasted. The animal lived 18½ days after the appear-
ance of the hæmatozoon in the blood which consisted of one long paroxysm. Death on the 23rd
August 1893 at 10.15 A.M.
Précis of experimental cases inoculated with the blood of different animals, which had been
previously inoculated from the Dog.
Two Guinea Pigs were inoculated on the 7th September, each with 0.25 c.c. of blood, taken
from the heart of Horse No. XCIII, 8 hours post mortem. Previous to death the hæmatozoa were
swarming. The temperatures of both animals never exceeded that of health, and as the parasite had
not made its appearance in the blood by the 9th October 1893, taking temperatures was discontinued,
but the blood was examined miscroscopically for a further period of 63 days, making 101 in all.
Cat II was inoculated on the 7th September 1893, with 0.5 c.c. of blood taken from the
heart of Horse No. XCIII, 8 hours post mortem. Previous to death the hæmatozoa were swarm-
ing. The temperature of the animal never exceeded the normal limits of health until the 6th to 8th of
October, but again quickly fell. The investigation was carried on for a further period of 59 days,
but as up to that time no hæmatozoa had appeared in the blood the experiment was discontinued : —
TABLE XLVI.
Showing details of animals inoculated with blood obtained from the Dog (spontaneous Surra) and passed
through other animals from the dog.
Appendix
No.
I
II{
III{
No.
...
XCIII
I
II
II
I
II
Kind of
animal inocu-
lated.
Naturally,
contrated.
Dog
Horse ...
Guinea Pig ...
„ „ ...
Cat
Guinea Pig ...
„ „ ...
Fluid used for purpose
of inoculation.
...
Blood.
„ ...
Quantity used for pur-
pose of inoculation.
...
1.25 c.c.
0.25 c.c.
0.25 c.c.
0.5 c.c.
0.25 c.c.
0.25 c.c.
Fluid used taken from
what animal.
...
Dog
„
„
Horse xclii.
„ „ ...
„ „ ...
Date of Inoculation.
...
25-7-93
25-7-93
25-7-93
7-9-93
7-9-93
7-9-93
How administered.
...
Subcut. ino
„
„
„
„
Inoculation with fluid
taken ante mortem.
...
...
...
...
Number of Hæmatozoa
present in fluid ante
mortem.
...
...
...
...
...
...
...
Inoculation with fluid
taken post mortem.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
Number of hour fluid
taken post mortem.
...
1.5
1.5
1.5
8
... ...
Number of Hæmatozoa
present in fluid taken
post mortem.
...
VN
VN
VN
A
A
A
Result of Inoculation
Positive or Negative.
...
P
P
P
N
N
N
Date of 1st appearance
of Hæmatozoa
in the
Blood.
...
4 8 93
3 8 93
5 8 93
...
...
...
Number of days after
inoculation 1st appear-
ance of Hæmatozoa in
the Blood.
...
10
9
11
...
... ...
Result of Inoculation.
Death
„
„
„
...
...
...
Date of Death.
25 7 93
7 9 93
21 8 93
22 8 93
...
...
...
Days between inocula-
tion and Death.
Remarks.
...
44
27
28
...
...
...
B 2204—37