97
Kidneys present a healthy appearance. Liver.—Signs of old perihepatitis. Stomach presents a healthy
appearance. Small Intestine.
The duodernum and a small portion of the jejunum present an œdema¬
tous condition of their walls, but there are no extravasations of blood or signs of ulceration in the whole
length of the gut. Colon presents a small patch of congestion and there are a number of small worms
lying on the mucous membrane, but no ulceration existed.
(b). SUBCUTANEOUS INOCULATION WITH BLOOD DERIVED FROM POSITIVE CASES.
Results Positive—Horses Nos. XXXV, XXXVI, XLV, LXVII, XXXVII.
Horse No. XXXV.—Chestnut
Country-Bred, aged. Inoculated subcutaneously on the 2nd August
1891, with 0.5 c.c. of blood containing numerous hæmatozoa taken from the jugular vein of Horse
No. XXVIII. The latent period was six days. A raised tumour appeared at the seat of inoculation
on the day following the injection, which continued tense and tender until the time of the organism
appearing in the blood of the general circulation. The temperature remained within the normal limits
of health until death which occurred on the second day of the disease.
Autopsy.—Body fairly nourished. Lungs.—Consolidation of the anterior portion of both organs,
together with sub-pleural extravasations. Pericardium.—The
sac contained a few ounces of fluid.
Heart.—Base surrounded by yellow gelatinous exudation, sub-pericardial extravasations. On section,
the auricles present a few sub-endocardial ecchymoses, whilst there are very extensive sub-endocardial
extravasations in the right and left ventricles, especially the former. Liver.—Fairly healthy.
Spleen.—
Normal in size, numerous sub-capsular petechiæ. Kidneys.—Pale, and cortical vessels congested.
Stomach.—Villous portion presents a lump due to the ' Spiroptera Megastoma'. Small
Intestines.—
Petechiæ aggregated together in groups, but no ulceration. Large Intestines.—Healthy
in appearance.
Horse No. XXXVI.—Chestnut
Country-Bred, aged. Inoculated subcutaneously on the 2nd
August 1891 with 0.5 c.c. of blood containing very numerous hæmatozoa taken from the jugular vein
of Horse No. XXVIII. The latent period occupied five days. A raised swelling appeared at the seat
of inoculation the day following the injection, which continued to be tense and tender until the appear-
ance of the organism in the blood of the general circulation. The temperature remained sub-normal
during the whole time the case lasted, rose on the first day and attained a maximum of 38°C. on the 3rd
day of the disease.
Autopsy.—Body fairly nourished. Lungs.—The tissue of the apices presented a semi-solid condition
and the organs being-dotted over with sub-pleural extravasations. Pericardial sac contained no fluid.
Heart.—Numerous and extensive sub-pericardial extravasations. There are a few small sub-endocardial
extravasations involving the walls of both ventricles. Liver—Signs of old perihepatitis, tissues healthy.
Spleen enlarged, numerous sub-capsular extravasations. Kidneys.—Pale in colour, the stellate veins in-
jected and congestion of the Malphigian corpuscles. Two Spiroptera Megastoma tumours involving the
villous portion. Small Intestines.—Œdematous
condition of the walls of duodernum and upper portion
of the jejunum, also a few small patches of congestion dotted over the mucous membrane. Large Intes-
tine.—Deeply congested patches but no ulceration.
Horse No. XLV.—Brown
Country-Bred, aged. Inoculated subcutaneously on the 6th October
1891 with 1.0 c.c. of blood containing swarms of hæmatozoa taken from the jugular vein of Horse
No. XLII. The latent period occupied sixteen days. Swelling and œdema followed at the seat of in-
oculation on the day following, but had entirely disappeared twenty-four hours later. The temperature
commenced to rise immediately after inoculation, attaining a maximum of 39.7°C. on the fourth day;
from this time until the appearance of the hæmatozoon in the blood it continued to oscillate presenting
an intermittent type. For the first five days of the disease the temperature assumed a remittent type
with morning remissions and exacerbations in the evening ; it then became very irregular and finally
death took place on the tenth day of the disease.
Autopsy.—Body fairly nourished. Lungs.—The apices were the seat of a localized emphysema ;
there were also numerous sub-pleural petechiæ. The pericardium sac contained a few ounces of blood-
stained fluid. Heart.—The base of the organ was the seat of a considerable amount of orange-coloured
exudation together with extensive sub-pericardial extravasations, especially along the course of the
coronary vessels. Small sub-endocardial extravasations in all the cavities with the exception of the
right auricle. Liver.—Considerably enlarged, signs of old perihepatitis, and on section numerous con-
cretions were found invading the tissues. Spleen.—Double the normal size, sub-capsular petechiæ.
Kidneys.—Normal in appearance. Stomach, healthy. Small Intestines.—Isolated patches of congestion,
no ulceration. Large Intestine, healthy. Parasites.—A few specimens of Ascaris Megalocephala.
Horse No. LXVII.—Grey
Country-Bred, aged. Inoculated subcutaneously on the 21st September
1891 with 1.0 c.c. of blood containing a few hæmatozoa taken from the jugular vein of horse No. LXII.
The latent period occupied nine days. Slight swelling and œdema followed at the seat of inoculation
on the day after the injection of blood, and remained until the hæmatozoon was discovered in the
general circulation. The temperature remained sub-normal until the sixth day after inoculation when
it attained 39°C. in the evening, falling to 37.4°C. in the morning, but on the evening of the 7th day it
rose to 38.8°C. and became gradually elevated until it attained a maximum of 40°C. on the morning of
the second day of the disease. The animal succumbed the same evening,
B 2204—25