120
ance of tumour 18th day. Hæmatozoon appeared in the circulation on the 64th day after inoculation, or
46 days subsequent to the disappearance of the local changes.
SUBCUTANEOUS INOCULATION WITH BLOOD DERIVED FROM POSITIVE CASES.
Horse No. XXXV.—Inoculated
with 0.5 c.c. blood, hæmatozoa very numerous. The swelling
reached a maximum on the evening of the sixth day, reduction in size and tension of the tumour,
occurred on the morning of the eighth day, and the organism was then discovered in the blood. Death
on the ninth day.
Horse No. XXXVI.—Inoculated
with 0.5 c.c. blood, hæmatozoa very numerous. Maximum
amount of swelling on the fifth day. Reduction in size and tension of tumour on the morning of the
sixth day ; organism discovered in the blood at the same time. Death ninth day.
Horse No. XLV.—Inoculated with 1.0 c.c. blood taken 2½ hours post mortem ; hæmatozoa swarm-
ing at time of death. Swelling marked on the second day, but on the morning of the third every sign
of it had entirely disappeared. The hæmatozoon was not discovered in the circulation until the 17th
day. Animal fell dead suddenly on the 31st.
Horse No. LXVII.—Inoculated
with 1.0 c.c. blood, containing few hæmatozoa. Swelling in-
creased up to evening of 10th day. On the morning of the 11th day, sudden reduction in size and ten-
sion of the tumour. Hæmatozoa discovered in the blood. Death on the 12th day.
Horse No. XXXVII.—Inoculated
with 0.7 c.c. blood taken 7½ hours post mortem ; hæmatozoa
very numerous just previous to death. Swelling and tension increased to P.M. on the 6th day.
Morning of 7th day reduction of tumour ; hæmatozoon first observed. Death on the 8th day.
With one exception, immediately the hæmatozoon made its appearance in the blood, reduction in
the size and tension of the tumour took place.
SUBCUTANEOUS INOCULATION WITH SOILED RAT'S BLOOD.
Results Negative.
Horse No. LVII.—Inoculated
with 2.0 c.c. rat's blood, hæmatozoa numerous. The swelling
attained a maximum on the 4th day; all trace of the tumour disappeared on the 18th day after
inoculation.
Horse No. LII.—(i) Inoculated with 2.0 c.c. rat's blood, hæmatozoa numerous. Swelling over
left scapular attained a maximum on the 13th day, reduction of the tumour occupied three days. (ii)
Re-inoculated with 2.0 c.c. of rat's blood, hæmatozoa numerous. Swelling increased up to 9th day,
reduction occupied two days only.
Horse No. XLIV.—Inoculated with 1.0 c.c. of rat's blood, hæmatozoa swarming. Swelling
attained a maximum on the 12th day. Reduction followed on the 13th, and by the 15th day only
little thickening left.
SUBCUTANEOUS INOCULATION AND RE-INOCULATION, AT A LATE
BLOOD, WITH NEGATIVE RESULTS ; FURTHER INOCULATION OF TH
ANIMAL WITH SOILED HORSE SURRA BLOOD.
Results Positive.
Horse No. XIII.—(i) Inoculated 0.6 c.c. rat's blood, hæmatozoa numerous, directly into the
jugular vein. (ii) Re-inoculated subcutaneously with 1.0 c.c. of rat's blood, hæmatozoa numerous.
Swelling attained a maximum on the 11th day. (iii) Inoculated with Horse Surra blood 1.0 c.c.,
taken during an intermission. Swelling and tension increased up to the 8th day ; on the 9th day
hæmatozoon appeared in the blood, and tumour became reduced in size, &c. Tumour measured ½ inch in
diameter on the 14th day.
Horse No. XII.—(i) Inoculated subcutaneously 1.0 c.c. rat's blood, hæmatozoa numerous. Swell-
ing attained a maximum on the 4th day, and then remained stationary until the 8th day, later reduction
took place. (ii) Re-inoculation, after 23 days, with 2.0 c.c. rat's blood, containing very numerous
hæmatozoa. Swelling attained maximum on the 4th day and remained stationary ; reduction later.
(iii) Inoculation on muzzle with one minim of Horse Surra blood, hæmatozoa numerous. No swelling
at seat of inoculation observed until 6th day; tumour 1/3 rd inch in diameter, 7th day increased in size and
very tender on pressure. Tumour reduced in size on the morning of the 8th day.
Horse No. XLVIII.—(i)
Inoculated subcutaneously with 0.75 c.c. rat's blood, hæmatozoa numerous.
Swelling attained a maximum, 3 inches diameter, on 2nd day. Reduction followed. (ii) Re-inoculat-
ed subcutaneously, in two places, with 0.75 c.c. rat's blood, hæmatozoa numerous. The swelling on the
right side attained its maximum dimensions 3½ × 2½ inches on the 2nd day; remained stationary up
to the 7th day, and had entirely disappeared on the 10th day. The tumour on the left side attained a
maximum on the 2nd day—3 × 2 inches—and diminished in size on the 3rd day. (iii) Inoculated
with 0.5 c.c. Horse Surra blood, no organisms, during an intermission of the disease. Not until the
9 th day did a slight thickening occur at the seat of inoculation, otherwise nothing to point out the
situation. The hæmatozoon was discovered in the blood on the 11th day. Death occurred on the
same day.