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intermissions of 109, 9 and 58 days, respectively. The animal contracted rinderpest on the 26th
September 1893, and death took place on the 28th.
Donkey No. II.—This animal was subcutaneously inoculated on the 31st July 1892, with 1.0 c.c. of
blood, containing very numerous hæmatozoa, taken from the jugular vein of Horse No. LXVII. This
horse had been inoculated from Horse No. LXII, which had been injected with blood directly from the
rat (Mus decumanus). The latent period occupied four days. The animal lived 144 days, after the
appearance of the organism in the blood, during which period twenty-one paroxysms and twenty inter-
missions occurred. The longest time during which a paroxysm lasted was seven days, the shortest one
day; intermission ten days, the shortest one day, giving an average length of nearly 3.3 days for the
former and 3.75 days for the latter. The temperature during the first 27 days of the disease varied
between 41.4°C. and 39.2°C., but on the 28th day fell to 38°C. From this latter date until the 120th day
of the disease, periodical attacks of fever occurred lasting five to six days, the temperature rising from
normal to 41°C. or over, and again falling to 38°C. or thereabouts. The third stage was marked between
the 120th and 144th days, on which latter date death took place by well marked remissions in the
morning and exacerbations in the evening, which towards the close of the case became exaggerated,
especially on the 12th, 13th, 14th, and 24th December, when the temperature varied as much as from
3°C. to 4.4°C. (5.4° to 7.9°F.). The hæmatozoa were present during this period for sixty-nine out of
one hundred and forty-four days.
Autopsy.—Body emaciated. No swelling or œdema about the extremities or under-surface of
the abdomen. Thorax.—No fluid in the pleural cavities, and no adhesions of the pleuræ. Lungs.—Small,
localized emphysema of the apices and inferior borders of both organs; a few lobules presented an atalec-
tatic condition; tissues fairly healthy. Pericardium.—The sac contained half an ounce of a clear, amber-
coloured fluid. Heart.—The base of the organ was surrounded by light yellow exudation, which also-
surrounded the origin of the great vessels, and was dotted over by a few purple-coloured petechiæ. All
the cavities presented a healthy appearance with the exception of the left ventricle which contained a
few sub-endocardial extravasations. Liver.—Healthy. Spleen.—Normal in size, a few sub-capsular
petechiæ; tissues healthy in appearance. Stomach contained no food, but one ounce of a yellow-coloured
fluid. Cuticular portion was healthy in appearance. Villous portion presented a strip, three inches
wide from the line of limitation, of a bright rose colour, but there was no solution of continuity. A tumour
2.5"× 1.5" × 1" occupied the fundus of the organ, and on section numerous Spiroptera megastoma were
presented to view. Dotted over the mucous membrane were also 24 circular raised plaques with a
thickened, pale-looking edge around the circumference of each; some are cupped on the surface, whilst
others have lost their epithelial covering. Small intestines present a few small petechiæ scattered over
the surface of the mucous membrane. At the commencement of the jejunum, a small circular and
somewhat raised tumour was found, which on section presented a small cavity in its interior, 1/8 inch in
diameter, and filled with healthy-looking pus. Cæcum healthy, but numerous small " Strongyli." Colon
presented several patches of congestion dotted over the walls, but no solution of continuity. Two
Guinea Pigs were injected subcutaneously with 2.0 c.c. of urine on the 8th August 1892, with negative
results.
Cow No. I.—This animal was obtained on 24th August 1892, with a calf at her side three weeks
old. She was giving 2 to 3 seers of milk a day. The experiment determined upon had for its object the-
following, viz:—(i) when Surra is communicated to a cow in milk, does the hæmatozoon or its
immature form pass from the general circulation into the milk ? (ii) will a calf suckling its mother under
such conditions contract the disease ? The animal was inoculated subcutaneously on the 28th August
1892 with 1.0 c.c. of blood containing very numerous hæmatozoa drawn from the jugular vein of
Donkey No. II. The period of incubation occupied five days. No swelling or œdema was observed
at the seat of inoculation, although a slight thickening could be felt in the subcutaneous tissue. Four
paroxysms lasting 5, 1, 1 and 2 days, respectively, were followed by three intermissions lasting 55, 18
and 21 days, respectively. The milk from the first day of the appearance of the Trypanosoma in the
blood was daily submitted to a searching microscopical examination, but on no occasion was a single
hæmatozoon discovered. On the 9th February 1893, as 58 days had intervened since the Surra
hæmatozoon had been last observed in the blood, and as the animal appeared to be in good health, it was
decided to re-inoculate it, with a view to determine whether any protection had been conferred by the
first attack. Consequently, at 6 P.M., on the last-named date, the animal was subcutaneously inoculated
with 20 c.c. of blood containing numerous hæmatozoa taken from the jugular vein of Horse No. LXXXIV
immediately after death. The latent period occupied 8.5 days and no local manifestation was observed
at the seat of the injection. From the first appearance of the organism in the blood on the 18th
February 1893 until 31st May 1894, six paroxysms each lasting from 1 to 2 days occurred. The
hæmatozoon never exceeded two in a field of the microscope (Zeiss.0c. 3, Obj D). The length of the
intermissions was 10, 29, 70, 9, 128 days, respectively, but as the examination of the blood was only-
discontinued on May 31st, 1894, 214 days after the last appearance of the organism in the blood, it
is very unlikely that the hæmatozoon would have again appeared in the circulation. On this latter date
the animal was in good health, and in very fair condition.
Two Guinea Pigs were inoculated subcutaneously with 0.5 c.c. of blood each, containing a few
hæmatozoa, taken from the ear of the cow during paroxysm V of the second inoculation period.
Guinea Pig No. I.—The period of incubation occupied five days. The organisms were present for
five days in the circulation, on the last of which the animal succumbed. Temperature varied between
35° and 40°C.