136
In this case if further injections of serum had been administered, a prolongation of the latent period
might have occurred or even a cure might have been established. The difficulty of obtaining bandicoots
was considerable, and out of 15 caught in December, 1894, only two presented the hæmatozoon in their
circulation :—
TABLE XLIII.
Experimental cases.
Appendix Number.
1
..
Number.
I...
IV...
III...
I...
I...
II...
I...
III...
IV...
V...
II...
II...
Kind of Animal inoculated.
Guinea Pig
Donkey ...
Mule
Rabbit ...
„
...
„
Guinea Pig.
Rabbit ...
„
„
Guinea Pig.
Rabbit ...
Fluid used for the purpose of
inoculation.
Blood .
„ ..
Quantify of fluid used for
inoculation.
0.2 c.c.
0.6 c.c.
3.25 c.c.
{ 2.40 c.c.
2.60 c.c.
2.5 c.c.
{ 2.5 c.c
0.2 c.c.
0.1 c.c.
0.1 c.c
0.1 c.c
0.3 c.c.
0.2 c.c.
0.2 c.c.
0.2 c.c.
0.4 c.c.
From what Animal fluid used was
taken.
Bandi-
coot IV.
} Bandi-{
coots.
„
IV.
„ XIII.
„ XIII.
„ XIII.
Rabbit I.
Guinea
Pig I.
„ ...
„
...
„...
Date of Inoculation.
5th
August
1804.
12th Septem-
ber 1894.
9th Decem-
ber 1894.
15th Janu-
ary 1894.
17th
Janu-
ary 1895.
3rd January
1805.
} 22nd
Janu-
ary 1895.
5th
August
1894.
15th August
1894.
Do.
Do.
23rd Septem-
ber 1594.
10th Septem-
ber 1894.
Do.
Do.
..
15th Septem-
ber 1894.
How administered.
Subcut-
ancous :
inocula-
tion.
} Do. ...
}Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ..
Inoculation with fluid taken ante
mortem.
...
...
...
...
...
...
...
...
...
...
...
...
Number of Hæmatozoa
present
in
fluid ante mortem.
...
..
...
...
...
...
...
...
...
...
...
...
Inoculation with fluid taken post
mortem.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
P.M.
Number of hours fluid taken
post mortem.
Imme-
diately.
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do. ...
Do....
Do. ...
Do. ...
Number of Hæmatozoa
in fluid
taken
post mortem.
S.
V.N
{ S.
S.
S.
{V.N
V.N
S.
S.
S.
S.
A.
A.
A.
A.
A.
Result of Inoculation, Positive or
Negative.
P.
}N.
}N.
N.
N.
N.
N.
N.
N.
N.
N.
N.
Date of first appearance of Hæ-
matozoon
in the blood.
9th Au-
gust 1894.
...
...
...
...
...
...
...
...
...
...
Number of days after inoculation
lst appearance of Hæmatozoa
in the blood.
4
...
...
...
...
...
...
...
...
..
...
...
Result of Inoculation.
Death
...
...
...
Death
Do.
Do.
Do.
Do.
Do.
Do.
Date of death.
15th Sept-
ember1894.
...
...
...
23rd Sept-
ember 1894,
25th Octo-
ber 1894.
10th Sept-
ember 1894.
25th Octo-
ber 1894.
20th Octo-
ber 1894.
17th Octo-
ber 1894.
3rd October
1894.
Days between inoculation and
death.
41
...
...
...
...
...
...
...
...
...
...
Remarks.
Spirillum.
Do.
Do.
Do.
Do.
Do.
Do.
SECTION VII.
SMALL ANIMALS INOCULATOD FROM THE BANDICOOT CONTAINING A MINUTE SPIRILLUM.
The blood of the majority of Bandicoots caught in Poona was found to contain an exceedingly
minute spirillum during the rainy season, even when the Trypanosoma was present in the circulation.
Rabbits and Guinea Pigs inoculated subcutaneously with blood containing this organism, contracted the
disease, and death resulted in the great majority of cases. This spirillum is exceedingly small, and
attention was first called to it in the blood of a Bandicoot, as the blood corpuscles presented an irregular
movement, and occasionally, minute moving specks were visible in the clear serum spaces between the
corpuscles. Never more than five spirilla were observed in a field at one time. Small quantities of
Bandicoot blood were injected subcutaneously into Rabbits and Guinea Pigs. The latent period in
Rabbits was in five instances 35,16,16, 20 and 14 days respectively. The period taken up between the first
appearance of the spirillum in the blood and death was occupied in the case of the Rabbits by paroxysms
during which the organism was present, and by intermissions during which it was absent from the blood-
The former varied in length from 1 to 16 days, and the latter from 1 to 17 days. The animals lived from
16 to 37 days. The latent period in Guinea Pigs averaged 24.5 days, and the animals died on the day
that the spirillum made its appearance in the blood.