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No. II was inoculated subcutaneously on the 7th December 1890, with 0.2 c.c. of blood from a
similar source to No. I. No symptom of any kind was noticed until 8th March 1891, when the animal
presented a muco-purulent discharge running from both nostrils, and the eyelids glued together caused by
the drying of the purulent secretion coming from them. Several peculiar-shaped bodies were observed
in the blood on the 12th April, but the hæmatozoon was never discovered in it. The animal eventually
regained its normal health. Result negative.
Two rabbits Nos. III and IV were inoculated in the tunieæ vaginales testes on the 23rd July 1892,
with 0.5 c.c. of blood containing numerous hæmatozoa taken from the hearts of two rats (Mus
decumanus) immediately after death.
No. III died two days later of general peritonitis, but no hæmatozoa were discovered in the blood
or peritoneal fluid.
No. IV.—Three days after inoculation, the scrotal sac became red, swollen and the surface tempe-
rature raised, and on the 16th day following the injection of blood, suppuration ensued and a caseous
mass escaped. The wound healed, and the animal appeared to have regained its normal health, but
succumbed on the 4th September, 42 days after inoculation. The hæmatozoon was not discovered in the
blood. Result negative.
Guinea Pigs inoculated with the blood of the Rat (Mus decumanus).
Two Guinea Pigs Nos. I and II were inoculated subcutaneously on the 22nd November 1891, with
0.5 c.c. of blood containing numerous hæmatozoa taken from the hearts of two rats (Mus decumanus)
immediately after death. In both cases their blood was examined up to March 9th, 1892, but with nega-
tive results.
Guinea Pig No. III was subcutaneously inoculated with the same quantity of blood from a like
source on 19th September 1892, and as by the 30th October, no symptoms of Surra had supervened, it
was re-inoculated with 0.2 c.c. of blood in each inguinal region. Enlarged glands followed in the
neighbourhood of the seats of the injection of blood. But no hæmatozoa were discovered on micros-
copical examination. Death, however, took place on the morning of the 12th November 1892.
Fowls inoculated with Rat's (Mus decumanus) blood.
Two fowls (Gallus domesticus) were each inoculated subcutaneously on the 23th January 1891,
with 0.3 c.c. of blood containing numerous hæmatozoa taken from the heart of a rat immediately
after death. As up to June 12th, no symptoms referable to the inoculation had supervened, the birds
were re-inoculated with blood swarming with hæmatozoa, taken from the jugular vein of Horse No. XIII
post mortem, just previous to death. The blood in each case was daily submitted to microscopical
examinations, but with negative results.
(f) S U B C U T A N E O U S I NOCUL AT I ON WITH THE SOILED BLOOD OF DI FFERENT ANI MALS, WHICH HAD BEEN PREVIOUSLY INOCULATED FROM THE RAT.
(i) Result Positive—Bull No. III, Donkey No. II, Cow No. I, Cat No. I, Dog No. I,
Horse No. LXIX, Rabbit No. II.
Bull No. III.—Age 4 years. This animal was subcutaneously inoculated, on the 31st July 1892,
with 1.0 c.c. of. blood containing very numerous hæmatozoa, taken from the jugular vein of Horse
Mo. LXVII, but previously from the rat (Mus decumanus). The latent period occupied three days.
The hæmatozoon was present in the blood from the 3rd to the 9th August inclusive. After an interval
of thirteen days, it was again present in small numbers for two days, August 23rd and 24th. After
a second interval of nine days it re-appeared for one day, on the 3rd September. During a further
period of 158 days, accompanied by daily microscopical examinations of the blood, no organisms were
observed. The temperature became rapidly elevated after inoculation and attained a maximum of
41.4°C. on the fourth day of the disease, and then gradually fell until the morning of the 8th day, when
it registered 38.8°C. It again rose to 41°C. on the 37th day. The pyrexia, since November 14th, has
been characterised by morning remissions and evening exacerbations, especially on the 143rd, 155th,
156th, 168th, 178th days of the disease. During the months of August and September this animal be-
came greatly emaciated, but by December had put on a considerable amount of flesh and appeared quite
healthy.
Two Guinea Pigs, inoculated on the 21st September 1892, with 0.3 c.c. of blood, in which no
hæmatozoa could be discovered, after a latent period of 14 days, contracted Surra.
As 191 days had elapsed since the animal was subjected to the injection of Rat Surra (through
horse) blood, and 158 since the last appearance of the hæmatozoon in the blood, on the 3rd September
1892 it was decided to re-inoculate with Horse Surra blood, in order to test whether any protection
had been acquired from the previous inoculation. Consequently, the animal was subcutaneously
inoculated at 5-30 P.M. on the 9th February 1893, with 2.0 c.c. of blood containing numerous
hæmatozoa, just before death, but drawn from the jugular vein of Horse LXXXIV immediately
death occurred. No swelling or œdema followed, but on the 4th day there was a slight thickening of
the tissues at the seat of inoculation. Three paroxysms occurred, lasting 1, 3 and 3 days, with three