132
(v) 22nd January 1895, inoculated subcutaneously on muzzle trace of blood containing very
numerous hæmatozoa.
(vi) 3rd April 1895, inoculated subcutaneously 2.0 c.c. blood containing very numerous
hæmatozoa.
(vii) 8th April 1895, inoculated subcutaneously 7.5 c.c. blood containing numerous hæma-
tozoa.
Between the 5th and 6th inoculations after a period of observation the animal was submitted to a
course of Iodides of Arsenic and Mercury, with two objects in view: (i) to observe the changes which
occurred in the constituents of the urine, during and after the administration of the drug, as also with
regard to the corpuscular elements of the blood; (ii) to render the animal, if possible, more susceptible
to the organisms of the bandicoot at the succeeding inoculation.
Rabbit No. I.—Inoculated with 0.2 c.c. of blood, containing swarms of hæmatozoon taken from
the heart of Bandicoot No. IV immediately after death. Blood examined from August 5th to Novem-
ber 6th, 1894, but no hæmatozoa were found in the blood.
Rabbit No. II.—Inoculated
with 0.4 c.c. of blood, taken from the heart of Guinea Pig No. I, im-
mediately after death. Blood microscopically examined from September 15th to November 19th, 1890.
No hæmatozoa observed.
Rabbit No. III.—Inoculated
with 01 c.c. of blood, containing swarms of hæmatozoa, taken from
heart of Bandicoot No. XIII, immediately after death. Blood examined from August 15th to October
25th, 1894, on which latter date the animal succumbed. No hæniatozoa. A minute spirillum was
observed.
Rabbit No. IV.—Inoculated
with 0.1 c.c. of blood, containing swarms of hæmatozoa, taken from
the heart of Bandicoot No. XIII, immediately after death. Blood examined from August 15th to Sep-
tember 23rd, 1894, when death occurred. No hæniatozoa, but spirillum found. Vide Appendices
page 203.
SECTION V.
PATHOLOGICAL CHANGES.
The post mortem examinations of bandicoots proved of great interest. Nematode worms varying
in numbers and dimensions (½ to 5 inches in length) were found in the stomachs of these animals, in
many instances completely distending the organs. In a total of 150 animals examined, nematodes were
found in 90 or 60 per cent.
The livers of these animals were also the seat of extensive changes, due to the presence of vast
numbers of nematode eggs (Trichocephalus dispar), some of which mature in situ, and frequently the
young worms escape into the peritoneal cavity.
The chief pathological changes observed were ulceration (sometimes extensive) of the villous por-
tion of the stomach, and as an effect of the former, the formation of diverticula, the walls of the sac
being formed of thickened peritoneum, extravasations of blood under the submucous tissues ; enlarged
spleen—4 or 5 times the normal size—and less frequently abscess of liver, spleen and subcutaneous
tissues.
As in the case of pregnant rats, so in that of pregnant bandicoots, although the Trypanosoma was
present in vast numbers in the blood of the mother, in no instance has it been discovered in the circu-
lation of the fœtus.
TABLE XXXIX.
Showing the total weight in grammes of the Body weights and Spleen weights of Bandicoots
examined
in the blood of which the Trypanosoma was present or absent during ten months, together with the
Ratio of Spleen to Body-weight.
Month, year.
1894.
July
August
September
October
November
December
HÆMATOZOA PRESENT.
Body-weight.
Grammes.
238.0
1,059.0
1,140.0
1,573.0
416.0
739.0
Spleen.
weight.
Grammes
0.63
7.42
6.74
7.27
1.70
7.10
Ratio Body-
weight
to Spleen-
weight.
1:377
1 :143
1:167
1 :216
1:245
1:105
HÆMATOZOA ABSENT.
Body-
weight.
Grammes
460.0
12,533.3
3,569.0
8,353.0
3,559.0
8,358.0
Spleen.
weight.
Grammes
1.71
42.86
17.46
24.45
7.30
31.10
Ratio Body-
weight
to Spleen
weight.
1:269
1 :292
1:204
1:342
1:487
1:268
NUMBER OF
BANDICOOTS.
Present.
1
5
4
6
2
2
Absent.
1
32
10
19
5
13