100
Autopsy.—Body emaciated, extremities swollen and œdematous. Lungs the seat of extensive sub-
pleural petechiæ and concretions. The pericardium sac contained several ounces of saffron-coloured
fluid. Heart.—Apparently
healthy. Liver.—Old signs of perihepatitis. Spleen.—Enlarged, seat of a
few sub-capsular petechiæ. Kidneys.—Present
a healthy appearance. Stomach.—This organ contained
a large tumour the work of the Spiroptera megastoma. Intestines healthy.
Horse No. XLVIII.—White
Country-Bred gelding, aged. Inoculated subcutaneously on the 15th
October 1891, with 0.75 c.c. of blood containing numerous hæmatozoa taken from the heart of a rat (Mus
decumanus) immediately after death. Swelling and œdema together with tension and tenderness of the
part followed at the seat of inoculation, but no hæmatozoa appeared in the blood up to the 30th, conse-
quently re-inoculation with 1.0 c.c. of blood containing numerous organisms from similar source was
performed on that date. The same local symptoms followed as on the previous occasion, but again the
blood remained healthy.
On November 12th, it was decided to inoculate with Horse Surra in order to observe whether any
modification of the disease would be produced in consequence of the previous injections of rat's blood.
The animal was, therefore, inoculated with .5 c.c. of blood which was free from the hæmatozoon, having
been taken from Horse No. L during an intermission. From the 15th to the evening of the 29th of
October the temperature maintained a certain degree of constancy varying between 38.4. o C. and 37°C. but
on the morning of the 21st touched 36.2°C. During the second period between the 30th October and the
11th of November the temperature attained a slightly higher limit, viz., between 38.8°C. and 36.8°C.
And during the third period, the incubative period of the Horse Surra, 40°C. was reached, the minimum
range being 35.6°C. The latent period occupied ten days. The animal succumbed on the second day of
the disease.
Horse No. XLVI.—Black
Country-Bred, entire, aged. Inoculated subcutaneously on the 15th October
1891, at 5 P.M., with 2.0 c.c. of blood containing numerous hæmatozoa taken from the hearts of two rats (Mus
decumanus) immediately after death. Marked swelling and œdema together with tension and tenderness
of the part on manipulation followed at the seat of injection. The œdema disappeared on the 23rd
instant, leaving a hard raised tumour, which lasted on for a week or ten days longer. As no symptoms of
disease supervened it was decided to inoculate the animal with Horse Surra blood. Consequently, on the
23rd of November, 1.0 c.c. of blood drawn from the heart of Horse No. LXVIII, one hour and a quarter
post mortem, was used. Swelling and œdema followed at the seat of the second injection of blood four days
later accompanied by the usual symptoms. After an incubative period lasting eight days the hæmatozoon
made its appearance in the blood of the general circulation. From the 15th October to the 22nd
November the temperature presented an almost intermittent type, there being, in most instances, a consi-
derable difference between the morning and evening record, but it only on four or five occasions
exceeded the normal limits. Between the 23rd November and the appearance of the organism on the
1st December, the thermometric readings varied between 35.6°C. and 38.5°C.,but on the second day of the
disease attained a maximum of 40.1°C., and then gradually fell to 36.8°C. in twenty-four hours, finally
oscillating until death which took place on the 7th December, the seventh day of the disease.
Autopsy.—Body emaciated, visible mucous membranes are yellow in colour, considerable œdema
of the extremities and exudation under the belly. Lungs presented a yellowish red colour together with
sub-pleural petechiæ. Heart.—The base of the organ is surrounded by yellow-coloured exudation.
Large and extensive sub-pericardial extravasations as well as numerous small petechiæ were found on the
right ventricle and running down with the anterior coronary vessels. There were sub-endocardial extra-
vasations in all the cavities with the exception of the left auricle. Those in the ventricles were the
most extensive, especially in the left. Liver.—Enlarged,
tissues tinged a yellow colour. Spleen.—En¬
larged to two and a half times the normal size; numerous sub-capsular petechiæ. Kidneys tinged yellow,
Horse No. LVIII.—Country-Bred
horse,aged. Subcutaneously inoculated on the 18th February
1892 with 2.0 c.c. of blood containing very numerous hæmatozoa taken from several rats (Mus decumanus)
immediately after death. Considerable swelling and œdema appeared at the seat of inoculation, accom¬
panied by tension and tenderness of the part. On the 9th March the swelling had all disappeared. On
the 29th of the same month, no symptoms having supervened, the animal was inoculated with 1.0 c.c. of
blood taken from the heart of Horse No. XLVII five and a half hours after death. The blood contain¬
ed swarms of hæmatozoa ante mortem.
Swelling and œdema with the accompanying tension and tender¬
ness of the parts intervened, but the latter disappeared at the advent of the organism in the general
circulation after a latent period lasting nine days. On the morning of the second day of the disease an
urticaria appeared on the head and neck which later became general in character but commenced to
fade towards evening and had entirely disappeared on the following morning.
The temperature was marked, during the period which elapsed between the 18th February and
29th March, by daily remissions and exacerbations which, on several occasions, varied over 3°C. between
the maximum and minimum. From the appearance of the hæmatozoon until death which took place
on the 3rd May, the twenty-seventh day of the disease, a continued fever accompanied by remissions and
at irregular intervals by well marked intermissions was present.
Autopsy.—Body somewhat emaciated. Swelling and œdema of the extremities and under-surface
of the abdomen. Lungs.—Localized emphysema at the apices, extensive sub-pleural ecchymoses. Fluid
in the pericardium.
Heart, sub-pericardial extravasations. No endocardial complication.
Liver.—Old
perihepatitis.
Stomach.—No pathological changes; numerous spiroptera. Spleen.—Enlarged,
sub¬