135
TABLE XLII.
Showing the date, temperature, and amount of Serum administered, together with details with
regard to the microscopical examination of the blood.
GUINEA PIG.
Date.
December
„
„
„
„
„
„
„
„
January
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
1894.
23
24
25
26
27
28
29
30
31
1895.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
How administered.
Inoculated 0.48 c.c. Horse Surra
blood.
Subcutaneous injection of Bandi-
coot Serum.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Death during t
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
do.
he night
Quantity
of Serum
injected
c.c.
...
1.52
0.80
1.81.
...
...
2.48
...
2.40
2.40
1.25
...
...
...
...
0.30
Nil
„
„
„
„
„
„
„
Temperature.
A.M.
...
...
38.0
37.8
38.4
37.6
37.4
38.8
40.0
38.0
38.4
38.4
37.4
37.4
38.0
38.6
38 4
38.7
37.6
37.8
38 6
37.4
38.0
37.8
37.4
...
P.M.
...
38.8
38.6
38.5
39.6
39.6
40.2
40.2
39.8
40.6
40.3
39.6
40.1
39 .8
40 .2
40.0
40.3
39.8
40.4
39.5
39.8
39.4
38.8
39 2
39.6
...
Hæmato-
zoa.
Absent.
„
„
„
„
Few
„
„
„
„
Numerous
„
„
„
„
„
Few
„
„
„
„
„
„
„
„
„
Remarks.
1 hæmatozoon in cover-glass
specimen of blood.
White corpuscles increased in
number.
Ditto.
Ditto.
12,000 hæmatozoa in cover-glass.
14,000
ditto.
14,000
ditto.
With reference to the injection of serum it will be as well to mention that according to Table
XI, Vol. I, Surra Report, " showing the latent period of Surra ascertained experimentally in different
animals," the period of incubation when inoculated from soiled Horse Surra blood taken during life
and containing numerous hæmatozoa, in two instances in the Rabbit, occupied 5 and 9 days respectively,
giving an average of 7 days, while in three Guinea Pigs the latent period occupied 11, 9 and 7 days
respectively, or an average of 9 days. In the above tables it will be seen that in the case of the Guinea
Pig the hæmatozoon appeared on the 6th day or about 120 hours after inoculation, when a total of
4.16 c.c. of serum had been injected, whereas in the control animals noted the average period was 216
hours. It would therefore appear that in the case of the Guinea Pig the effect produced by the serum
was rather to shorten the latent period by five days or to render the animal more suceptible to the action
of the Trypanosoma.
The most marked effect produced was upon the course of the disease, for instead
of paroxysms and intermissions taking place, the organism after its first appearance in the blood, re-
mained present until death occurred. The hæmatozoa present were however very small in number and
never attained to anything like the proportion usually noticed in Surra in the Guinea Pig.
The effect produced in the case of the Rabbit was essentially different. We have observed that
the period of incubation occupied in the control animals of the same species inoculated with Horse Surra
blood containing hæmatozoa, was on an average 7 days duration. A total of 20.79 c.c. of Bandicoot
serum was injected subcutaneously during this experiment on the 2nd, 3rd, 4th, 7th, 9th, 10th, 11th
and 17th days after the inoculation with Surra blood, the only effect produced being an increase in
the number of white cells in the blood on the 7th and 8th days and again from the 23rd to the 26th
days inclusive. During the last 19 days of the life of the animal only one injection of serum was made.
Forty-eight hours before death occurred, the 29th clay after inoculation, the Trypanosoma made its
appearance in the blood in very small numbers, but on the day of death there was a further increase.
The result therefore of the serum injection in the case of the Rabbit must be looked upon as a distinct
success, for the period of incubation instead of occupying 7 took 29 days ; a retardation of 22 days.