101
capsular petechiæ. Kidneys petechiated. Small Intestines.-Mucous
membrane petechiated, no ulcera-
tions. Large Intestines healthy.
(e) SUBCUTANEOUS I NOCULATI ON OF ANIMALS OTHER THAN HORSES WITH SOILED RAT'S BLOOD.
(i) Results Positive-Bull
No. IV, Monkey No. II, Field Rats Nos. I and II.
Bull No. IV—was inoculated on July 27th, 1892, with 2.0c.c. of blood containing the hæmatozoon
taken from the rat (Mas decumanus) immediately after death. A considerable amount of swelling and
œdema followed at the seat of the injection, which gradually decreased in extent and finally disappeared
about the 10th August 1892. From the 27th July until October 10th, 1892, the blood remained free
from the hæmatozoon. On the evening of the 9th September an urticaria broke out on both sides of
the neck, chest and scapular regions, but had disappeared entirely by the following morning. On the
morning of the 3rd October 1892, a similar eruption to the former made its appearance and again faded
the same day. On microscopical examination of the blood on the 11th October, the 76th day after
inoculation, a long and active 'Trypanosoma' was discovered, the dorsal membrane being particularly
well developed. From that date up to the 9th February 1893, the 121st day of the disease, although the
blood was subjected to careful examination daily, no further hæmatozoa were observed.
The temperature on the morning of the 8th day following inoculation attained a maximum of 41°C.,
but by the following morning had fallen 2°C., and although it subsequently on numerous occasions
touched 40°C. it has only once risen to 40.3°C. From November 16th the pyrexia was characterized by
marked morning remissions and evening exacerbations especially on the 55th, 56th, and 59th days of
the disease when the morning and evening temperatures varied from 2.5°C. to 3.°C. (4.5° to 5.4°F.). The
animal lost flesh during the months of August, September, October and November, but at the present
time (January 1893) is beginning to put on flesh.
Two Guinea Pigs inoculated on the 21st September 1892 with blood from this animal, in which no
hæmatozoa could be discovered, failed to contract the disease.
As it was now February 9th, 1893, 197 days since the animal was subjected to the injection of rat
(Mus decumanus) blood and 121 days since the last appearance of the hæmatozoon in the blood, it was
decided to inoculate with Horse Surra blood in order to test whether any protection had been acquired
from the previous inoculation. Consequently, the animal was subcutaneously inoculated at 5-30 P.M.
with 2.0 c.c. of blood containing numerous hæmatozoa, drawn from the jugular vein of Horse LXXXIV
immediately after death. No swelling followed this operation, but on February 14th, 1893, the muzzle
became hot and dry, and there was considerable fever lasting several days, but the hæmatozoon was not
discovered although the blood of the animal was collected and examined microscopically three times daily
during the period the fever lasted. On the 30th of April, 80 days after the re-inoculation, the hæma-
tozoon was observed in small numbers and remained in the blood for 48 hours, after which it disappear-
ed and was absent until the 18th July 1893, a period of 78 days, when it was again present in small
numbers for one day. From this date until the 29th January 1894, a period of 195 days, when the
animal was killed in consequence of having met with an accident, the hæmatozoon was never found
although daily examinations of blood were made.
Probably hæmatozoon was present in the blood in small numbers during the presence of the
fever.—February 14th, 1893.
Monkey No. II.—(Macacus Rhesus) was subcutaneously inoculated in the right inguinal region
on 23rd March 1893 with 1.50 c.c. of blood containing numerous hæmatozoa taken from the heart of a
rat (Mus decumanus) immediately after death. A slight thickening followed the injection at the seat of
inoculation. The latent period occupied three days. The organisms appeared in the blood in small
numbers for one day only, although the microscopical examination of that fluid was continued daily until
23rd July 1893, a period of 120 days. The temperature during the days of observation varied between
38.2° and 39.3°C., after inoculation it oscillated between a maximum of 41.0° and a minimum of 37°C.
Field rats (Nesokia providens) inoculated with blood from the rat (Mus decumanus).
Two field rats
were subcutaneously inoculated on the 13th February 1891, each with blood containing numerous
hæmatozoa taken from the hearts of two rats (Mus decumanus) immediately after death.
No. I received 0.6 c.c. subcutaneously. The latent period occupied seven days. The hæmatozoon
was present in the blood off and on until May 26th, 1891, on which day it succumbed, 102 days after
inoculation.
Autopsy.—Spleen large, otherwise the organs appeared healthy.
No. II received 0.4 c.c. The latent period occupied as in the case of No. I seven days. The hæma¬
tozoon was present off and on until 31st July 1891, on which date death took place, 168 days after
inoculation.
Autopsy.—The spleen was enlarged, otherwise the organs presented a healthy appearance.
(ii) Results Negative.
Donkey No. III, Monkey No. I, Cat No. II, Rabbits Nos. I, II, III, IV,
Guinea Pigs Nos. I, II, III, Fowls Nos. I, II.
Donkey No. III.—This animal was in fair condition, but aged. It was kept under observation and
the blood examined daily from 28th January until 9th March 1893, on which latter date it was sub¬
B
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