BALWANT
SINGH
113
According to the reaction found in these tests,
13 animals were classified as groups A and B mixed.
Of these, 12 animals (1, 2, 3, 5, 6, 7, 8,12, 17, 20, 21
and 23) were then selected and their isonins and
isogens cross-tested. The results, given in Table
II, indicate eight animals belonging to A group
and four to B group.
TABLE II
Analysis of bloods with A and B mixed groupings
selected from Table I
(1)
Isogen
from
hill
bull
1
2
3
5
6
7
8
12
17
20
21
23
(2)
Isomin from
1
—
+
±
—
—
—
±
—
—
+
—
2
±
—
—
+
+
+
+
+
—
—
—
3
+
+
—
+
+
—
—
—
+
+
—
5
+
+
+
—
+
—
+
+
+
+
—
6
—
±
—
—
—
—
+
—
—
+
—
7
—
+
±
—
—
+
+
—
—
+
—
8
+
+
+
—
+
—
+
+
—
+
±
12
+
—
—
—
+
—
—
+
—
—
+
17
+
+
±
—
—
—
±
—
+
+
—
20
—
±
—
—
—
±
±
—
—
—
—
21
+
+
—
V
—
—
—
—
—
—
—
23
+
+
±
—
+
—
+
—
+
+
—
(3)
Grouping
A
B
B 1
A 1
A
A
A 3
B 1
A 2
A
B 2
A 2
It is also noticed that the animals of both groups
showed reactions against animals of their res-
pective groups, thus suggesting the presence of
sub-groups or serveral antigenic compo-
nents of cattle cells. The problem was further
studied by an absorption test conducted in the
following way.
Equal quantities of packed cells from group
A animals and serum from group B, and vice versa,
were well mixed in a tube, left at room temperature
for 30 minutes, then at 37°C. for an hour and
finally at room temperature for 30 minutes.
Since the serum titre was found to be very low,
the amount of serum was subsequently increased
to two, four and nine times the volume of packed
cells. From this it was found that the optimal
ratio for complete absorption of homologous
isonins was 1:5. By absorption tests the follow-
ing distribution of groups was found:
Groups
A
A 1
A 2......
A 3
B
B 1
B 2
Hill bulls
1, 6, 7, 20.
5.
17, 23.
8.
2.
3, 12.
21.
Thus in Kumauni hill cattle the ratio of groups
A to B appears to be as 2:1.
2. Red Sindhi. The home of this breed is the
province of Sind about Karachi, Hyderabad, the
coastal line and the Indus river banks, including
the Las Bela areas of Baluchistan. The animals
are of red or fawn colour, frequently with some
white on the face and dewlap. They are among
the best milch type cattle of India and the bullocks
are useful for draught. The animals are short—
horned.
The tests were made in January and February,
1942 on 96 cattle (92 cows, 4 bulls) at the Imperial
Dairy Institute, Bangalore, where a suitable herd
of Sindhi cattle is maintained. It is a self—contain-
ed herd and the animals are therefore regarded
as inbred for the purpose of this study. The
results obtained with this and most of the
other breeds are given in Table III.
3. Gir. The home of this breed is the Gir forest
in Junagadh State (Kathiawar), where these cattle
are found in a pure state. The breed is also spread
over a large part of Bombay either pure or crossed
with local cattle. The animals have short horns.
The colour is generally mottled—red or black and
of various shades of these colours. In some strains
the body is mainly white with red or black spots,
but entire reds and blacks are also met with.
The cows are generally good milkers but the bulls
are slow and lethargic in work. The Gir is re-
ported to be the best beef animal in India.
The blood group investigations were also carried
out at the Imperial Dairy Institute, Bangalore,
where a small herd of these cattle is maintained
and at the Palace Dairies of Bhavanagar and