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three paroxysms lasting 32 days and two intermissions lasting 4 days. The third paroxysm occupied 28
days when death took place.
A utopsy.—Organs appear healthy. Body weight 347 grammes, spleen weight 1.7 grammes, ratio
of spleen weight to body weight =1 : 204.
(i) TRANSMISSION OF THE HÆMATOZOON FROM THE MOTHER TO THE O
THROUGH SUCKLING.
Result Positive.—Calf No. I.
Calf No. I.—This animal was about twenty-one days old when it was brought at the side of its
mother. The cow was inoculated on the 28th August 1892 with Surra, and the hæmatozoon appeared
in its blood on September 2nd, and remained present until the evening of the 6th. The calf was allow-
ed to suck its mother three times daily, but during the intervals was tethered at some distance from,
but in sight of, the cow. The milk from each teat was subjected to microscopical examination for a
considerable period, but no sign of the hæmatozoon was discovered. Up to the morning of the 9th
October, the 38th day from the appearance of the organism in the blood of the mother, no organisms
had been observed in the blood of the calf, but on that date hæmatozoa of some length were found,
which were also very active in their movements. From that day until the 9th February 1893, no more
were observed, although careful examination of the blood was made daily. A point of considerable
interest is observed on reference to the charts of the mother and the young one. In the case of the latter,
the falls in temperature of the mother are faithfully recorded on that of the calf, and when the tempe-
rature of the cow became markedly of an intermittent type, it was recorded, and if anything, rather
exaggerated in the temperature curve of the young one. The calf on February 9th, 1893, was in good
condition and had grown considerably. It was, therefore, decided to re-inoculate it with Surra, with a
view to determine what course, if any, the disease would take. Consequently, at 5 P.M. on the above date
1.0 c.c. of blood was subcutaneously injected, taken from Horse No. LXXXIV immediately after death.
The hæmatozoon was numerous ante mortem. The latent period occupied only three days. The orga-
nism was present for two days, 13th and 14th February, and again for one day, 18th, after an intermis-
sion of 72 hours. From 18th February until September 30th, 1893, a period of 224 days, the organism
was present in the blood once and then only for a few hours, viz. on the 22nd June, 124 days after
its last appearance. The animal spontaneously contracted rinderpest, the first symptoms appearing on
the 28th and succumbed to that disease on September 30th, 1893.
(j) SUBCUTANEOUS INOCULATION OF GUINEA PIGS WITH URINE PASSED BY ANIMALS
DURING A PAROXYSM OF SURRA.
Negative Results.—Guinea Pigs Nos. I, II (Bull No. III). Guinea Pigs Nos. I, II
(Donkey No. II).
Two Guinea Pigs Nos. I and II were each injected subcutaneously on the 8th August 1892 with
2.0 c.c. of urine passed one hour previously by Bull No. III. in whose blood hæmatozoa were swarm-
ing at the time. The urine was first allowed to settle for one hour, the supernatant liquid was then
poured off and the remaining few cubic centimetres used for the purpose of injection.
No. I.—The hæmatozoon was absent during the period which elapsed between the injection and
death which occurred on the 26th August after an interval of 18 days.
No. II showed no symptoms whatever referable to the urine, and after September 30th the blood
was no longer submitted to examination. Results negative.
Two Guinea Pigs Nos. I and II were each injected subcutaneously with 2 c.c. of urine passed
one hour previously by the donkey in whose blood the day previous hæmatozoa were swarming.
The urine was first allowed to settle for one hour, the supernatant liquid was then poured off, and the
remaining few cubic centimetres used for injection purposes.
No I showed no symptoms referable to the injection of urine, and after 30th September the
blood was no longer submitted to examination.
No. II.—The animal showed no symptoms referable to the injection of urine, but twelve days
after inoculation developed epidemic diarrhœa at that time prevalent and succumbed to it on
August 21st, 1892. Results negative.
B 2204—28