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coloured fluid. Heart—5 lbs. 10 ozs., external surface presented a few sub-pericardial extravasations.
On section the cavities contained coloured blood clots, and the right ventricle a few sub-endocardial extrava-
sations. The other cavities presented a healthy appearance. Abdominal cavity was free from fluid.
Liver, normal in size. There were signs of old perihepatitis on the right lobe. On section tissues healthy,
Spleen enlarged—double normal size— presented a few sub-capsular petechiæ, tissues soft. On section
kidneys presented a healthy appearance. Stomach.—The villous portion was invaded by a small spiroptera
tumour, otherwise healthy. Intestines and Bladder present a normal appearance.
Morse No. XL VII.—This animal was subcutaneously inoculated on the 15th October 1891, over
the left scapular region with blood containing hæmatozoa mixed with normal saline solution. The
blood was removed immediately after death from the heart of a large sized specimen of " Barbus
carnations." A circular raised swelling appeared on the following day at the seat of the injection of
blood and continued to increase in size, until the evening of the 17th, after which it remained stationary
until the 19th, and then gradually became less, and finally disappeared. No general symptoms of disease
supervened, so the animal was re-inoculated on the 29th October, with blood from a similar source. Again
swelling and oedema followed at the seat of the injection, the former measured 5" × 4" on the morning of
the 31st, but by the 2nd November had again disappeared to a considerable extent. In this instance
also no symptoms supervened and the blood, which was examined daily, maintained a healthy condition.
On the 19th November, the animal was again re-inoculated with blood from a specimen of " Barbus
camaticus " as before. No hæmatozoa were found in the blood, but on the 9th, 10th, 11th and 12th
December, several Næmatode hæmatozoa were observed daily. The tempeiature from October 8th until
the beginning of March, on only one occasion registered 40°C, and that was on the 16th November;
it, however, for long periods, showed marked morning remissions and evening exacerbations.
On March 9th, 1892, this animal was finally inoculated with 1.0 c c. of Horse Surra blood, contain-
ing swarms of hæmatozoa, taken i'rom the jugular vein of No. LV. The latent period occupied five days.
On the third day after the injection of blood, a small raised tumour, the size of a walnut, appeared at the
seat of inoculation which gradually increased, and reached considerable dimensions, viz., 7" × 4. 5" on the
morning of the 13th ; but by the 14th, when the hæmatozoon was discovered in the blood, the tissues were
found to have lost some of the tension and tenderness previously observed. The temperature gradually
became elevated on the evening of the last inoculation, and steadily rose until noon on the fourth day of the
disease, when the thermometer registered 42.1°C. (107.7°F.), but by the morning of the 5th day had fallen
to 37.3°C, a difference of 4.8°C. (8.6°F.). The temperature again rose on the 7th to 40.6°C., and on the
10th to 41.5°C., and death took place on the 29th March 1892, the 16th day of the disease.
Autopsy at 9.30 A.M. on the 29th March.—Body fairly well nourished. There was slight œdema
of the sheath, and under-surface of the abdomen. Cavity of the Thorax contained no fluid, and there
were no pleuritic adhesions. Lungs pale pink in colour, localized emphysema at the apices, otherwise
healthy. Heart apparently healthy. Liver old perihepatitis, few calcareous bodies on the surface of the
organ. On section tissues healthy. Spleen enlarged to 2.5 normal size, no sub-capsular petechiæ
Kidneys present a healthy appearance. Stomach.—Cuticular portion healthy. Villous portion presents a
tumour 1.75" in diameter due to " spiroptera " (megastoma). Intestines present a healthy appearance.
Horse No. .XXXIX—This animal was subcutaneously inoculated on the 11th August 1891, with
blood containing hæmatozoa mixed with normal saline solution, taken immediately after death from three
specimens of " Rhynchobdella acideata." Baum (Hindustani) Warm (Marátha). A raised tumour fol-
lowed at the seat of the injection of blood, which was tense and tender on manipulation. It continued to
increase in size until the 15th, after which it became gradually reduced and finally disappeared. No h--
matozoa were discovered in the blood. The Temperature which at the time of inoculation was sub-normal,
36.2°C. (97°F.), commenced to rise on the 14th August, and attained a maximum of 38°C. on the evening
of 20th. It then gradually fell until death which took place on the evening of the 25th. Temperature
35.2°C. (95.3°F.).
Autopsy.—There was an entire absence of any pathological changes referable to the injected fish
blood.
Horse No. XLIX.—Black Mare. This animal was subcutaneously inoculated on the 15th October
1891, with a few drops of blood containing hæmatozoa mixed with normal saline solution. The blood
was removed from the hearts of several specimens of the " Rhynchobdella aculeata " immediately after
death. Some swelling and œdema followed at the seat of inoculation on the morning of the 16th,
which continued to iucrease in extent until the evening of the third day, the 17th, and then gradually
disappeared. As no symptoms had supervened up to the 29th, and the blood, during the whole time,
had been free from hæmatozoa, the animal was re-inoculated with blood obtained from a fish of the
same species as that used on the previous occasion. On the morning of the 30th well marked petechiæ
were present on both the conjunctival membranes, and there was some swelling and œdema at the
seat of inoculation, which latter rapidly disappeared in the course of 48 hours. Seven days later a
muco-purulent discharge was observed to escape from the right nostril, and continual gurgling was
present in the intestines. Between the 9th November and death, which occurred on the morning of
the 16 th, the animal was observed to be losing flesh; there was a yellowish tinge of the visible mucous
membranes, and a continual discharge from both nasal passages. At the same time there were fresh
crops of large petechiæ invading the membranæ nietitantes. During the last few days the animal
gradually became weaker and the appetite fell off.
The tempprature between the 15th and evening of the 28th of October oscillated between 38.6°C.
and 3.6.5 °C. After re-inoculation on the 29th October, the temperature was 39°C. on the evening of