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(h)—SUBCUTANEOUS INOCULATION WITH THE BLOOD OF DIFFERENT ANIMALS, WHICH HAD BEEN
PRIMARILY INOCULATED FROM HORSE No. XCI (RAT EXCREMENT INGESTION).
Positive Results.—Horse No. XCV, Cat No. III, Guinea Pig No. XXIII.
Horse No. XCV.—Bay, Country-Bred Mare, aged, in fair condition. Inoculated subcutaneously on
the 9th October 1893 with 1 c.c. of blood containing very numerous hæmatozoa, taken from the jugular
vein of a Guinea Pig immediately after death, which latter had been previously inoculated from Horse
No. XCI (Surra from rat excrement) on the 28th September 1893. The latent period occupied five
days. Some swelling and œdema was present at the seat of the inoculation, accompanied by tenderness
on manipulation. Next morning the tenderness disappeared and the swelling was found to be less tense,
the hæmatozoon was found in the general circulation. From the day of inoculation until the end of
the period of incubation, the temperature oscillated between 36.4° and 37.6°C. On the first appearance
of the Trypanosoma in the blood the temperature rose rapidly and at midday, on the second day of the
disease, registered 40.2°C. After this it gradually fell until on the fifth day of the paroxysm it was
38.4°C. Chloroform was administered and death took place on the afternoon of the 18th October 1893.
Autopsy at 3.30 P. M. on the same date. The body is fairly well nourished, adipose tissues stained
an orange colour. The pleural cavities contain a few ounces of a blood-stained fluid. Lungs pale in
colour, localized emphysema at the apices; several large sub-pleural extravasations of blood on both
surfaces and anterior lobe of the left organ, and a few small isolated patches on the superior surface
of the right. Pericardial sac contains 4 ozs. of a clear orange fluid.
Heart weighs 7 lbs. 15½ ozs. The base of the organ is surrounded by a considerable amount of an
orange-coloured gelatinous material, dotted over its surface by dark-red coloured peteehiæ, the external
surface of the muscular tissue is free from ecchymoses with the exception of a few involving the right
ventricle near the apex.
On section.—There are no sub-endocardial extravasations present, and the valves are healthy.
Liver weighs 14 lbs. 5½ ozs., somewhat enlarged, presents numerous, small hard, white-coloured
nodules in its substance which were found, on section, to involve the structures of the whole organ.
Stomach.—On section, Cuticular portion healthy in appearance, Villous mucous membrane pre-
sents a pink colour; no ulceration or spiroptera tumours.
Spleen weighs 5 lbs. 10 ozs., considerably enlarged, nearly three times the normal weight. Surfaces
dotted over with numerous small dark-purple coloured sub-capsular peteehiæ. On section the tissues
present a very dark-purple colour and are somewhat soft in consistency.
Kidneys.—Each weighs 1 lb. 6½ ozs., capsules strip easily, the cortical surfaces covered with very
numerous small circular extravasations, which are found on section to invade the deeper structures.
Small and Large Intestines.—Healthy.
Cat No. III.—This animal was full grown and apparently in good health and condition. On
30th June 1894, after being under close observation since the 24th of the previous March, it was in-
oculated with 0.15 c c. of blood, containing very numerous hæmatozoa, taken from a Guinea Pig, pre-
viously inoculated with blood from Horse No. XCI. The latent period occupied seven days. From
the first appearance of the Surra organism in the blood, 7th July 1894, until death, 24th November
1894, one hundred and forty-one days elapsed; these were made up of 18 paroxysms lasting 60 days
and 17 intermissions lasting 81 days; an average for paroxysms and intermissions of 3.3 and 4.76 days
respectively. During the period of observation lasting 89 days, the temperature of the animal varied
between 37.6° and 40.3°C. The highest reading during the whole length of the case was 40.8°C. and
this occurred once in paroxysm VII, and once in intermission IX.
On the 128th day of the disease this animal became convulsed and these lasted day and night un-
til the 132nd day evening on every two or three hours; but later on the 138th day of the disease the
convulsive attacks again seized the animal and continued once in every 10 to 15 minutes until death on
the 141st day.
Autopsy.—Body emaciated, no swelling or œdema of the limbs, both cornea opaque.
Thorax.—No fluid in the pleural cavities, no adhesions. Lungs.—Apices seat of localized emphy-
sema, numerous sub-pleural ecchymoses. Pericardial Sac contains two drachms of fluid. Heart pale,
otherwise healthy. Abdominal cavity free from fluid. Stomach and Liver present a healthy appear-
ance. Spleen enlarged, convex surface dotted over with small irregular-shaped extravasations.
Kidneys pale, cortical portion of each petechiated. Intestines healthy.
Brain membranes are pale, superficial vessels full of blood. On section, tissues appear healthy
some fluid in lateral ventricles.
Guinea Pig .No. XXIII,—This
animal was inoculated on 30th June 1894 with 0.15 c.c. of blood,
containing very numerous hæmatozoa, taken from a Guinea Pig No. XXI immediately after death.
The inoculated disease had been brought through Guinea Pigs XXI, XX, XIX, which latter was in-
oculated with blood from Horse No. CXI—disease produced from rat excrement in the food. The latent
period occupied seven days. From the first appearance of the Surra organism in the circulation on
July 7th, 1894, until death on August 11th, 1894, was thirty-six days. This period was made up of