95
temperature 100.2,° nil, evening temperature 101°; thirteenth day morning temperature 100.4°,
nil, evening temperature 101.6°; fourteenth day morning temperature 100°, nil, evening tempe-
rature 101°; fifteenth day morning temperature 99.8°, nil, evening temperature 100°; sixteenth
day morning temperature 99.6°, nil; and for a few days longer similar negative results.
(2) A small monkey (Macacus radiatus) with healthy aspect and blood; morning
temperature 101°, possibly not all the injected material reached the peritoneal cavity, as the
blood had coagulated and it may have been blood serum chiefly which penetrated deeply;
second day morning temperature 100°; 18 hours after experiment the animal seems well, no
local swelling, but abdomen slightly tender at site of puncture to the right of the navel; in
the first sample of blood taken from a digit 2 monads were detected very actively moving and
otherwise presenting all the aspects of the hæmatozoon seen the day previously in the rat; they
kept near to each other and were long watched: temperature at 1 P.M. was 100.6°, at 5 P.M.
temperature 102°; another active organism was seen in a fresh specimen of the blood from
another digit: temperature at 9 P.M. 100°: third day morning temperature 101°; a specimen
of the blood now taken showed a single active monad : at 1 P.M. temperature 100.6°, at 5 P.M.
temperature 102°; a flagellate organism like that of the rat was again found in the blood, as
before: at 9 P.M.. temperature 100.2°; fourth day morning temperature 101.4°; I failed to
detect any parasite; evening temperature 101.6°; no parasite seen, but much plasmic matter
and many white cells; at 9 P.M. temperature 99.6°; fifth day morning temperature 100.4°, nil,
evening temperature 102°; plasmic particles small, many : at 9 P.M. temperature 100°; sixth
day morning temperature 100.6°, nil, at 1 P.M. 100.4°, at 5 P.M. temperature 101°, nil, at 9 P.M.
temperature 100°; seventh day morning temperature 100.4°, nil, evening temperature 101.6°
and later 99.6°; eighth day morning temperature 100.2°, nil, but many plasmic particles, evening
temperature 101.4° and 99°; ninth day morning temperature 100.4°, nil, evening temperature
101.4° and 99.6°; tenth day morning temperature 100.2°, nil, evening temperature 102°, at 9,
P.M. temperature 100°: eleventh day morning temperature 100.8°, evening temperature 101.6°
and 99.6°; twelfth day morning temperature 100.4°, nil, evening temperature 101.6° and 99.4°;
thirteenth day morning temperature 100.4°, nil, evening temperature 102° nil; at 9 P.M.
temperature 100°; fourteenth day morning temperature 101°, nil, evening temperature 102°
and 99.6°; fifteenth day morning temperature 100.4° nil, evening temperature 101.6° and 99.8°;
sixteenth day morning temperature 100.4°, nil, evening temperature 101.6° and 100°; seven-
teenth day morning temperature 100.4°, nil; and subsequently this animal long remained well.
SECTION II.
PRECIS OF EXPERIMENTAL CASES.
(a).—SUBCUTANEOUS INOCULATION WITH SOILED RAT'S BLOOD.
Results Positive—Horses Nos. IX, XXVIII, XLII, LXII.
Horse No. IX.—Bay Australian Gelding, aged 15 years, inoculated subcutaneously on the 28th
December 1890 with 1.0 c.c. of blood containing hæmatozoa, taken from the heart and great vessels of a
rat (Mus decumanus) immediately after death. The latent period was nineteen days. Considerable
swelling and œdema was present at the seat of inoculation accompanied by tenderness on manipulation.
The temperature from the day of inoculation to the morning of the fourteenth day after, oscillated
between 39°C., and 36.4°C., but from the evening of the latter date, when it registered 36.6°C., until the first
appearance of the hæmatozoon, the temperature gradually rose day by day until it reached 39.3°C. But
from the first day until death, which occurred on the twentieth day of the disease, the fever was of a
continued type with exacerbations reaching 41.3°C., 40.8°C. and 40.1°C. on the 3rd, 8th and 13th days of
the disease respectively.
Autopsy.—Body fairly nourished. Lungs. —Localized emphysema at both apices and a few sub-pleural
extravasations. The pericardial sac contained a considerable quantity of amber-coloured fluid. Heart.—
Base of the organ surrounded with yellow-coloured exudation. Numerous sub-pericardial extravasations
especially on the posterior aspect of the left ventricle. On section.—Sub-endocardial extravasations in
the right ventricle mostly involving the interventricular septum. Sub-endocardial extravasations con-
siderably elevated and especially involving the musculi-papillares. Liver large, old signs of perihepatitis,
tissue apparently healthy. Spleen much enlarged, numerous sub-capsular petechiæ. Kidneys.—Nume-
rous small circular extravasations lying on the surface of the organs and dipping into the cortical
substance to a distance of 1/12th of an inch. Stomach healthy. Small and large Intestines healthy.
Horse No. XX VIII.—Chestnut Country-Bred. Inoculated subcutaneously on 2nd July 1891 at
3 P.M., with 1.5 c.c. of blood taken from the hearts of two rats (Mus decumanus) immediately after death.
The latent period was twenty-nine days. Considerable swelling and œdema occurred at the seat of
inoculation accompanied by conjunctival petechiæ and at a later date by swelling of the right testicle,
with fluid in the tunica vaginalis. The hæmatozoon appeared in the blood for the first time on the
twenty-ninth day. The temperature during the period of incubation oscillated between 39.6°C. and