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organism. I, like Dr. Lewis, gained the impression that it commonly advanced ; yet it is certain that
when more tranquilly performed, progression is really with the flagellate extremity in front, as is usual
in the other infusorial monads.
Inoculation experiments.—These
were as follows : the subcutaneous injection of healthy animals
with tainted blood of the rat: the animals so employed being the rat, dog, cat, horse, and monkey ; also
the intra-peritoneal injection of a healthy rabbit and a healthy monkey. The last named attempt
proved alone successful, but it may be useful to offer a brief record of the whole series of trials :—
(a) Experiments on rats:—1 and 2. A fragment 1/10 square inch large of fresh blood-clot was
introduced beneath the skin of the thigh of two animals with seemingly healthy blood. Second day
the blood of both preserves its normal aspect: so on the third, fourth, fifth, sixth, and seventh day.
One rat now escaped; the other continued well and was submitted to observation daily until the
sixteenth day, when, the result being so far negative, it was killed and inspected without the de-
tection of special morbid change. 3. A similar fragment of blood-clot from the heart of rat
which had just died with flagellate organisms still persisting in the circulation was introduced
beneath the skin of the thigh of an animal with healthy-looking blood. Evening temperature
101°; the animal looks well: second day morning temperature 101.8°; the blood unchanged;
evening temperature 102.4°, no change and no evident constitutional irritation with this rise : third
day morning temperature 101.8°, nil; evening temperature 102.4°, nil: fourth day morning temper-
ature 100.6°, nil, evening temperature 101.6°, nil: fifth day morning temperature 100.4°, nil, evening
temperature 101°, nil: sixth day morning temperature 100.4°, nil, evening temperature 101.4°, nil:
seventh day morning temperature 100°, nil, evening temperature 101.6°, nil: eighth day morning
temperature 100°, nil, evening temperature 101.6°, nil: ninth day morning temperature 99.8°,
evening temperature 101.2°: tenth day morning temperature 100°, nil, evening temperature 100°:
eleventh day morning temperature 100°, nil; evening temperature 101.2°; and the animal continued
well for five days longer.
(b) Experiments on the dog:—1. A healthy white puppy, a month old, had a few minims of
fresh rat's blood containing several monads injected in the thigh: evening temperature 100.6°;
no irritation of wound: second day morning temperature 100.6°; blood unchanged; evening
temperature 101°, nil: third day morning temperature 100°, evening temperature 101.2°, nil: fourth
day morning temperature 99°, nil, evening temperature 100.6°: fifth day morning temperature
100.4°, nil, evening temperature 101° : sixth day morning temperature 99.6° and evening temperture
100°, nil; and so on for five days longer. 2. A healthy black male pup was injected with similar
blood; evening temperature 101.4°; nothing morbid visible in its blood: second day morning
temperature 101°, nil, evening temperature 100°, nil: third day morning temperature 99°, evening
temperature 101.4°, nil: fourth day morning temperature 100°, nil, evening temperature 101°: fifth
day morning temperature 101.4°, nil, evening temperature 100.4°; sixth day morning temperature
100° and evening temperature 100.4°, nil; and so on for six days longer.
(c) Experiments on the cat:—1. A large black healthy male ; evening temperature 101°;
had inoculated at inner surface of thigh a minute clot of rat's blood filled with active organisms ;
second day morning temperature 98°; was again inoculated with a small clot from another rat just
killed, which contained many monads, this being done to ensure thorough trial; evening temperature
100°, nil ; third day morning temperature 98°, blood nil, the wounds scarred ; evening temperature
102°; animal feverish, but eats heartily and sites of inoculation not inflamed ; a few large granule¬
cells observed in the blood : fourth day morning temperature 98°; nothing abnormal seen in the
blood except a few large granule-cells ; evening temperature 101.6°; nothing abnormal in the blood
but some large plasm-particles and a few large granule-cells : fifth day morning temperature 99°,
nil, evening temperature 101°, nil; sixth day morning temperature 98°, nil, evening temperature
101°, nil ; seventh day morning temperature 99°, nil; evening temperature 99°; eighth day morning
temperature 100°: nil, evening temperature 101.6°: ninth day morning temperature 99° and evening
temperature 100.6, nil; for five days longer the temperature remained normal. 2. A healthy
fawn-coloured small cat; evening temperature 101.4°, had inoculated in the thigh on point of scalpel
a minute clot of blood from a rat which contained the monads: second day morning temperature
99°; blood unchanged ; evening temperature 100.2°; white cells many in the blood, which seemed
poor in quality ; third day morning temperature 99°, nil, evening temperature 100.6°, nil, white
cells many : fourth day morning temperature 99°; only leucocytes and small plasm-particles unusual;
evening temperature 101.6°, leucocytes and large plasm-particles; fifth day morning temperature
nil, blood healthier looking, evening temperature 99°, nil; sixth day morning temperature 99°, nil,
evening temperature 101.6°, nil: seventh day morning temperature 98°, evening temperature 103°,
animal feverish, in its blood nothing unusual, but many white cells and no other apparent cause
of the fever; eighth day morning temperature 100.4°, nil, evening temperature 100.6°: ninth day
morning temperature 100.4° and evening temperature 101.4°, nil: for five days longer the temperature
normal.
(d) Experiments on monkeys :—1. A small fresh-brought healthy animal; had at 10 A.M.
injected minims xx of infected blood of rat just before killed on each side of thighs; temperature
102° : at 5 P. M. temperature 103°, nothing abnormal seen in the blood; wounds unchanged ; animal quiet:
second day morning temperature 100.4°; nothing abnormal in the blood, evening temperature 101.6°,
nil; third day morning temperature 101° and evening temperature 102°, no visible change in blood,
but animal seems oppressed; fourth day morning temperature 100°, nil, evening temperature 101.4°,
B 2204—24