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cutaneously inoculated with 1.0 c.c. of blood, containing very numerous hæmatozoa, taken from the
rat (Mus decumanus) immediately after death. A swelling, tense and tender on manipulation, was noticed
at the seat of inoculation, which gradually subsided in the course of ten days. During the period of obser-
vation, the temperature varied between a maximum of 39.8° and a minimum of 35.4°C. From the time
of inoculation until death, which occurred on the 5th May 1893, the temperatures varied between 39.4°
and 35°C.; and during the last twenty days of life it became of a markedly intermittent type, the
morning and evening registration showing a difference of from 3 to 3.5 degrees Centigrade (5.4 to 6.3°F.).
Although the blood of this animal was submitted twice daily to microscopical examination, the
hæmatozoon was never discovered, moreover, no symptom in any way pointing to the existence of such
a disease became manifest during the course of the case.
Two Guinea Pigs, however, were each inoculated with 0.5 c. c. of blood, taken from the heart of
the donkey, at 11-30 A.M. on the 5th May 1893, three hours and 45 minutes after death, in which no
organisms could be found.
No. (i) Guinea Pig.—The hæmatozoon appeared in the blood on the 9th June 1893, after a period
of incubation lasting 35 days. Death followed on the 20th June, 46 days from the day of inoculation
and 11 days after the first appearance of the hæmatozoon in the blood.
No. (ii) Guinea Pig.—The result was negative.
Two Guinea Pigs Nos. (iii) and (iv) were subcutaneously inoculated with blood taken from No. (i),
immediately after death. The hæmatozoa were swarming just before the animal succumbed.
No. (iii) Guinea Pig received 0.2 c.c. of blood, taken from the heart of Guinea Pig No. (i), imme-
diately after death. Latent period occupied four days. Paroxysm lasted six days, ON the last day of
which the animal succumbed.
No. (iv) Guinea Pig received 0.2 c.c. of blood taken from the heart of Guinea Pig No. (i) imme-
diately after death. Latent period occupied a very few hours, for, the inoculation was performed at
12-30 P.M. on the 20th June 1893, and at 10 A.M. on the 21st June, when the microscopical examination
of the blood was made, hæmatozoa were found to be then present and continued for following eleven days,
on seven of which the organisms were swarming. From the first appearance of the organism in the blood
until death supervened, 82 days elapsed ; this period was made up of 4 paroxysms lasting 78 days and
4 intermissions lasting 4½ days.
Autopsy.—Body somewhat emaciated, no œdema. Thorax.—Pleural cavities free from fluid.
Lungs.—Emphysematous, sub-pleural extravasations. Heart healthy. Liver enlarged, very dark in
colour. On section, tissues contain fair quantity of dark-coloured blood. Spleen.—Slightly enlarged,
tissues firm. Kidneys.—Both organs petechiated, right organ to a greater extent than the left.
On section—Tissues pale. Bladder presents healthy appearance.
Monkey No. I (Macacus radiatus)—was subcutaneously inoculated on the 1st August 1891 with 0.5 c.c.
of blood containing numerous hæmatozoa, taken from the heart of a rat (Mus decumanus) immediately
after death. A slight thickening followed the injection of blood at the seat of inoculation. The tem-
perature on the evening of the 8th and morning of the 9th August reached 39.8° and 40°C. respect-
ively; but from the latter date until the morning of the 26th when it rose to 40.3°C., the temperature
remained normal. As no symptoms of Surra had developed, on the 2nd September the animal was re¬
inoculated with 0.5 c.c. of blood from a similar source. On the evening of the 14th of September the
temperature rose from 38.8° to 41.1°C. regaining the normal on the morning of the 16th. Similar eleva-
tions took place on the 17th, 21st, and 25th. On the latter date the animal appeared to be ill, but the
condition of the blood was unchanged. Two days later the breathing became quickened and a dull and
sleepy condition supervened, from which the animal never rallied.
Autopsy.—Body well nourished. Lungs.—There were a number of small purple-coloured sub-pleural
petechiæ dotted over the surface of the organs. The right inferior lobe was covered with a layer of
yellow lymph 1/12th inch in thickness. On section.—The tissue of both the lungs presented a healthy
appearance. Pericardium was distended with clear coloured fluid ; no pericarditis.
Heart.—Healthy.
Liver, Spleen, Kidneys, and Bladder healthy.
Cat No. II (Felis domesticus).—This animal, large, full grown, yellow in colour and very wild,
was inoculated at 5 P.M. on the 14th January 1893, with 0.5 c. c. of blood containing numerous
hæmatozoa, taken from the heart and large vessels of a rat (Mus decumanus) immediately after death.
The temperature during the period of observation averaged 38.4°C., after inoculation and for a period of
184 days during which it was taken three times daily it oscillated between a maximum of 40.8° and a
minimum of 36.6°C.
The blood was also daily submitted to miscroscopical examination from 14th January to 16th July
1893, but no organisms were discovered. Result negative.
Rabbits inoculated with Rat's (Mus decumanus) blood.
No. I was inoculated subcutaneously on the 7th December 1890, with 0.5 c.c. of blood, containing
numerous hæmatozoa taken from the heart of a rat (Mus decumanus) immediately after death. On
the following morning the temperature rose to 40.7°C., but no hæmatozoa appeared in the blood and the
animal was still living and in good health six months later. Result negative.