131
I
Serial
number.
1
2
3
4
5
6
7
II
Number in
Laboratory
Case Book.
123
120
117
118
112
109
111
III
Hæmatozoa
in blood
one cubic
millimetre.
664
2,400
35,500
36,000
101,000
293,000
349,550
IV
Number
of orga-
nisms
(abbre-
via-
tions).
F
F
S
S T
T
T
V
Red corpuscles
in blood one
cubic millimetre.
6.660,000
6,200,000
5,850,000
5,400,000
4,110,000
7,190,000
5,940,000
VI
White
corpuscles
in one cubic
millimetre.
12,600
19,400
6,600
3,600
10,500
12,500
32,600
VII
Ratio white
corpuscles to
red corpuscles.
1 : 528
1 : 319
1 : 889
1 : 1,500
1 : 391
1 : 590
1 : 182
VIII
Ratio
hæmatozoa to
red corpuscles.
1 : 10,030
1 : 2,583
1 : 164
1 :
150
1 :
40.7
1 :
21.5
1 :
16.9
IX
Body-
weight.
Grammes.
772
837
555
211
142
83
128
X
Spleen-
weight.
Grammes.
4.40
3.00
1.70
0.70
0.60
0.85
0.70
XI
Liver-
weight.
Grammes.
77.6
26.9
20.9
9.9
10.7
6.8
7.2
XII
Ratio spleen-
weight to
body-weight.
1: 175
1: 279
1: 326
1 : 301
1: 236
1 : 97.6
1 : 183
XIII
Ratio liver-
weight to
body-weight.
1 : 20.5
1: 31.1
1: 26.5
1: 21.3
1: 13.2
1: 12.2
1: 17.7
SECTION IV.
PRECIS OF CASES OF ANIMALS INOCULATED WITH SOILED BANDICOOT'S BLOOD.
A.—Result Positive.
Guinea Pig.—Inoculated subcutaneously on August 5th, 1894, with 0.2 c. c. of blood containing
swarms of hæmatozoa taken from the heart of Bandicoot No. 4, immediately after death. The period
of incubation occupied four days. The hæmatozoon appeared on the 9th of August and was present in
the blood during the 10th and 11th, but was absent on the 12th. After the one day intermission it
again appeared in the circulation for 24 hours, and then disappeared altogether; for it was not observed,
although daily microscopical examinations of the blood were made in the interval between the 14th
August and 16th of September, on which latter date the animal succumbed. Temperature record,
Appendix page 201.
Autopsy.—No marked pathological changes present. Body weight 194 grammes. Spleen weight
0.2 grammes. Ratio of spleen to body weight 1 : 970.
B.—Results Negative.
Mule III, aged, but in good health, was inoculated subcutaneously on the 16th October 1894 with
1.5 c. c. of blood containing very numerous hæmatozoa taken from the heart of a Bandicoot (No. IV),
immediately after death. Swelling appeared at the seat of inoculation on the 17th and gradually
increased up to the 19th, then gradually disappeared. The temperature of the animal kept within
normal limits between the above date and January 3rd, 1895, a period of 80 days, the blood examined
daily always presented a perfectly healthy appearance. The mule was re-inoculated on the latter date
with 2.5 c.c. of blood containing very numerous hæmatozoa taken from Bandicoot No. CIV imme-
diately after death. On the 4th a swelling 4×3 inches appeared at the seat of the injection of blood,
which disappeared by the 8th. During the following three weeks the animal showed no signs of
disease, so on the 22nd January 1895 the mule was inoculated for the third time by making a very
small incision in the skin of the mu zzle and placing between the edges of the wound some soiled
blood from a Bandicoot containing very numerous hæmatozoa. Thirty-seven days were allowed to
elapse, during which time the animal showed no symptoms of Surra and the blood examined daily
maintained a healthy condition. It was then decided to inoculate the mule with a slight trace of Surra
blood containing hæmatozoa from the horse. Consequently, on February 27th, 1895, a small scratch was
made in the skin of the muzzle and trace of soiled blood from Horse CXXV was placed on the surface
of the scratch. Slight thickening followed at the seat of the inoculation. The latent period occupied
nearly seven days. On April 27th, 1895, chloroform was administered to the mule and death followed.
Between the first appearance of the Surra Trypanosoma in the blood and death 53 days elapsed, made
up of five paroxysms lasting 5, 20, 4, 4 and 6 days, and five intermissions lasting 2, 5, 2, 3 and 2 days
respectively.
Autopsy.—Ordinary pathological changes of Surra noted.
Donkey No. IV.—This animal, aged 10 years, height 9½ hands, was in good health and condition.
Subcutaneous inoculation was performed at intervals as under, with blood derived from different ban-
dicoots, with a view to produce Surra, but always with negative results.
(i) 12th September 1894, inoculated subcutaneously 0.6 c.c. blood containing very numerous
hæmatozoa.
(ii) 9th December 1894, inoculated subcutaneously 3.25 c.c. blood containing swarms of
hæmatozoa.
(iii) 15th January 1895, inoculated subcutaneously 2.40 c.c. blood containing swarming
hæmatozoa.
(iv) 17th January 1895, inoculated subcutaneously 2.60 c.c. blood containing teeming
hæmatozoa.