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also opacity of right cornea. Urine passed involuntarily, acid, albumen 1/16th, 6 P.M., convulsed,
fore limbs extended and rigid. Death 9 P.M.
The maximum temperature 40.5°C. was registered during the third intermission, and the minimum
38.2°C. during the first paroxysm. The case lasted twenty-five days after the first appearance of the
Trypanosoma in circulation; this period was made up of three paroxysms lasting 10, 4 and 8 days
and two intermissions lasting 1 and 2 days respectively.
Autopsy at 9 A.M., 27th September 1892.
Body well nourished, right cornea opaque, left slightly milky ; on removing skin two glands near
symphysis pubis, one on each side half inch in length. On section glands pale.
Thorax.—Yellow-coloured gelatinous material under surface of sternum. Lungs localized emphy-
sema, sub-pleural extravasations of blood. On section and pressure, frothy mucus exudes from tissues.
Pericardial Sac.—Contains 28 c.c. straw-coloured fluid, few flakes of attached surface of left ventricle.
Heart externally no petechiæ. On section endocardium pale, no petechiæ. Stomach healthy. Liver
large, tissues fatty. Gall bladder distended, green fluid. Spleen, 90 grammes, considerably enlarged.
Capsule petechiated. On section tissues soft. Kidneys.—Right weighs 15 OZS.=42.45 grammes. Left
1.75 ozs.=49.52 grammes. Capsules strip easily, surfaces pale, petechiated and lobulated. On section
cortical portion pale and narrow. Intestines healthy appearance. Bladder ditto. Brain membranes
pale, tissues appear healthy, no fluid in the ventricles.
Dog No. II—A Pariah, aged three years, was subcutaneously inoculated on 1st January 1893, with
1.5 c.c. of blood containing very numerous hæmatozoa taken from the heart of a rat (Mus decumanus)
immediately after death. The blood of this animal was examined until death took place on the 18th
February 1893, a period of 49 days, but during this interval no hæmatozoa were observed. For the
first six weeks, the temperature registered within normal limits or sub-normal, but during the last week
of life the maximum recorded was 39.8°C. It is probable that if a Guinea Pig had been inoculated
with the blood of this dog just before or after death, the animal might have contracted the disease.
Dog No. III.—A Pariah, was inoculated subcutaneously on the 16th January 1893, with 1.0 c.c.
of blood containing swarms of hæmatozoa derived from the heart of a rat (Mus decumanus) immediately
after death. The temperature on one occasion, 3rd March 1893, registered 40.6°C., but the same
evening fell to 38.6°C. The blood of the animal was microscopically examined daily, with negative
results, from January 15th to April 12th, 1893, a period of eighty-eight days. After the latter date the
experiment was discontinued.
Dog No. IV.—A Pariah, some 6 or 8 months old, was subcutaneously inoculated on the 22nd
January 1893, with blood containing swarms of hæmatozoa derived from the rat(Mus decumanus)
immediately after death. The temperature on the evening previous to inoculation registered 39.8°C.,
but the blood was healthy. On the evening of the 24th, temperature 40°C. Death occurred at noon
on the 28th January. Temperature 36.2°C.
Autopsy.—Organs presented a healthy appearance.
Dog No. V.—A Pariah, ten months old, was subcutaneously inoculated on the 23rd January 1893
with 1.0 c.c. rat's blood containing swarms of hæmatozoa, taken from the heart immediately after death
of the animal. The temperature remained low until the 10th February ; for five days it rose, and
registered between 40.2° and 40.6°C. ; it then fell, but again rose before death which occurred on the
20th February 1893. No hæmatozoa were discovered in the blood, although daily microscopical
examinations of it were made.
Autopsy.—Enlarged spleen, no marked pathological changes.
Dog No. VI A.—A Pariah puppy, aged four months, was inoculated subcutaneously on 14th De-
cember 1895, with 0.4 c.c. of blood taken from the jugular vein of Horse No. 1952. The latent period
occupied three and a half days. The maximum temperature registered was 40°C. on the night of the
19th December, the minimum 38°C. on the evening of the 25th. The hæmatozoon was present in the
blood from the morning of the 18th, until death, which occurred during the night of the 29th and 30th
December.
Autopsy.—On December 30th, 1895, at 11-30 A.M. Spleen and liver enlarged.
Dog No. VII.—A Pariah puppy, four months old, was inoculated subcutaneously on the 14th
December 1895, with 1.25 c.c. of blood drawn from the jugular vein of a black Colt No. 1991,
suspected to be the subject of Surra. As at a later date, January 23rd, 1896, no hæmatozoa had appear-
ed in the circulation, and as the animal was in perfect health, it was re-inoculated at 5 P.M. on the
above date, by making a scratch on the thigh, and placing therein a trace of blood containing a few
hæmatozoa derived from Donkey No. VI. The latent period occupied 10.5 days. The maximum tem-
perature 40.6°C. was registered during the second paroxysm and third intermission, and the minimum
37.4°C. during paroxysm four. The animal lived thirty-eight days after the appearance of the organism
in its circulation. There were five paroxysms lasting 3, 5, 21, 1 and 5 days, and four intermissions
lasting 1, 2, 2, 1 days, respectively. Death took place on the 11th March 1896.
Autopsy.—9-30 A.M. Body fairly well nourished. Rigor mortis strongly marked. Thorax free
from fluid, no pleural adhesions. Lungs deeply congested in variegated patches, no consolidation.
Pericardial sac contains 170 c.c. of a blood-stained fluid. Heart, weight 66 grammes. External
surface of organ very pale. On section no sub-endocardial extravasations. Abdominal cavity free from
fluid. Liver, weight 389 grammes, enlarged. Tissues fatty. Spleen enlarged, 38 grammes. Tissues