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Guinea Pig No. II.—Period of incubation five days. Death on the 3rd day of the first
paroxysm. Temperature varied between 38.6°C. and 40.6°C. This appears to have been a very severe
type of Surra.
Guinea Pig No. III.—Inoculated with 0.4 c.c. of blood containing a few hæmatozoa taken from
the ear of the cow on the 28th October 1893. The period of incubation occupied five days. The animal
lived 71 days after the first appearance of the organism in the blood. Two paroxysms occurred lasting
68 days, and one intermission 3 days. The organisms were very sluggish on the day of death.
Guinea Pig No. IV— Was subcutaneously inoculated on 12th January 1894, with 0.3 c.c. of blood
taken from the heart of Guinea Pig No. III, 21 hours post mortem. The latent period occupied ten
days. The animal only lived 20 days after the first appearance of the organism in the blood. This period
was made up of three paroxysms lasting 13 days and two intermissions lasting 2 days. Organisms
were very sluggish on the day of death.
Guinea Pig No. V.—This animal was inoculated on the 11th February 1894, with 0.4 c.c. of blood
taken from the heart of Guinea Pig No. IV, 12 hours post mortem. No organisms were discovered in
the inoculated blood. The blood of this animal was examined microscopically until the end of March,
but no Surra organisms made their appearance in it. Result negative.
Cat No. I.—A young animal about three months old was subcutaneously inoculated on the 28th
August 1892 with 0.5 c.c. of blood containing very numerous hæmatozoa, taken from the donkey,
but originally derived from the rat (Mus decumanus). The latent period occupied five days, after
which the hæmatozoa were found to be swarming in the blood. For the first ten days of the disease the
temperature oscillated between 38.4° and 40.4°C, but from the latter date until death, which took
place on the fiftieth day of the disease, there was continued fever, and for the greater part of the
time a temperature which oscillated between 39.7°C. and 41.4°C. The hæmatozoon was present on
forty-five out of fifty days, and for the last twenty-four was never absent. A symptom of considerable
interest presented itself in this case towards its termination, viz. the appearance of localized œdematous
swellings of an erratic nature present in one part of the body one day and in another part on the
follewing day.
Autopsy.—Body somewhat emaciated. The extremities are not swollen or œdematous, although
there is some present in front of the chest and on the under-surface of the abdomen. There are several
dark red coloured glands undergoing caseation in the inguinal regions. Thorax.—No fluid in the pleural
cavities and no pleuritic adhesions. Lungs.—Localized emphysema especially at the apices. The pericar-
dial sac contains a small quantity of light amber-coloured fluid. Heart presents a healthy appearance.
Abdominal Cavity.—There is no fluid in the peritoneal cavity, but there are several enlarged glands lying
on either side of the lumbar vertebræ. Stomach presented healthy appearance. Spleen presented a
healthy appearance. Liver presented a healthy appearance. Kidneys presented a healthy appear-
ance. Intestines presented a healthy appearance. Bladder presented a healthy appearance. Urine in
the bladder contained ¼ albumen.
Dog No. I.—This animal, a little more than three months old, was inoculated, on 28th August 1892,
with 0.6 c.c. of blood containing very numerous hæmatozoa, taken from the jugular vein of the donkey,
previously passed from the rat (Mus decumanus) through the horse to the donkey. The latent period
occupied five days. Continued fever was present from the time of inoculation until death took place,
with the exception of two slight intermissions which occurred on September 3rd and 25th, The tem-
perature varied from 38.2°C. to 40.4°C. The hæmatozoon was present for twenty-two days out of the
twenty-five the disease lasted, cerebral symptoms supervened together with 1/16 albumen in the urine, and
death took place twelve hours later.
Autopsy.—Body well nourished. The right cornea was almost opaque. Two enlarged inguinal
glands. Thorax.—No fluid in the pleural cavities. Lungs.—Localized emphysema at the apices,
also numerous sub-pleural extravasations. On section, the tissues presented mottled appearance and
frothy mucus exuded on pressure. The pericardial sac contained one ounce of straw coloured
fluid and a few shreds of lymph were attached to the muscular structure of the heart anteriorly.
Heart—The external surface was free from sub -pericardial extravasations as also the cavities of
the auricles and ventricles on section. Abdominal cavity.—This cavity is free from fluid. Stomach
presents a healthy appearance. Liver presents a healthy appearance. Spleen was dotted over with
dark-coloured sub-capsular petechiæ. On section, tissues soft and dark in colour. Kidneys pale and
dotted over with numerous small purple-coloured petechiæ. Intestines present a healthy appearance.
Brain.—Membranes pale, superficial veins contain little blood. The surface of the hemispheres
presented a healthy appearance and there was no fluid in the lateral ventricles. Eyes.—Right cornea
more or less opaque, the left was slightly fogged.
Horse No. LXIX.—Australian mare, aged. Inoculated subcutaneously, on the 3rd September
1892, with 0.5 c.c. of blood swarming with hæmatozoa taken from the cow. The latent period occupied
five days, a small circular and raised tumour appeared at the seat of inoculation on the day following
the injection and continued to increase for four days and then remained stationary ; during this time
there was tenderness on manipulation and marked tension of the part. On the morning of the sixth day
the tension and tenderness had decreased and a few hæmatozoa were discovered in the blood of the
general circulation. During the incubation period the temperature gradually rose and was marked by
well marked morning remissions and evening exacerbations. On the first day of the disease at the
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