Handbook of endocrine research techniques /
De Pablo, Flora.
Scanes, C. G.
Weintraub, Bruce D.
San Diego ; London : Academic Press,
c1993.
Includes bibliographical references and index.
Radioimmunoassay and radioreceptor assay: past, present, and future / Sonia M. Najjar and Bruce D. Weintraub -- Immunoradiometric assays / M.R. Pandian and D.A. Fisher -- Enzyme-linked immunosorbent assays / Endre V. Nagy and Kenneth Burman -- Development and characterization of polyclonal and monoclonal antibodies / Joan Rener -- Isolation of polypeptide hormones: general separation methods and some applications for thyroid hormones / Jorge Alemany, Jorge Garcia de Ancos and Enriques Mendez -- Reverse hemolytic plaque assays: applications to endocrine problems / L. Stephen Frawley -- Capillary electrophoresis coupled to fluorescence detection for the determination of multiple neuropeptides / Juan P. Advis ... [et al.].
(cont)   Immunoreactive heterogeneity in peptide measeurement / I. Valverde and M.L. Villanueva-Penacarrillo -- Why use a flow cytometer for endocrine cell analysis?  Frank M. Perez, Daniel R. Deaver and Wesley C. Hymer -- Identification and characterization of insulin-like growth factor-binding proteins / Yvonne W.-H. Yang and Matthew M. Rechler -- Localization of peptides: double labeling immunohistochemistry / Jose E. Garcia-Arraras -- Electron microscopic immunocytochemical approaches to the localization of ligands, receptors, transducers and transporters / Robert M. Smith and Leonard Jarett -- In situ hybridization histochemistry / Carolyn A. Bondy, Jian Zhou and Wei-Hua Lee.
(cont)   Plasma membrane isolation strategies for cell surface receptors: application for the insulin receptors / Maxine A. Lesniak, Joshua Shemer and Phillip Gorden -- Analysis of autophosphorylation and substrate phosphorylation by receptor tyrosine kinases / Robert S. Garofalo -- Involvement of protein tyrosine phosphatase activity in the regulation of insulin's signal transduction / Dalit Hecht and Yehiel Zick -- Techniques for evaluating protein kinase C in intact cells / Perry J. Blackshear -- Identification and quantification of G-proteins / Ravi Iyenga -- Determination of adenylyl cyclase catalytic activity / Roger A. Johnson and Yoram Solomon.
(cont)   Intracellular mediators of peptide hormone action: glycosyl phosphatidylinositol/inositol phosphoglycan system / Isabel Varela-Nieto, Luis Alvarez and Jose M. Mato -- Free calcium measurements: down to the single-cell level / Antonio Sanchez-Bueno and Peter H. Cobbold -- Approaches for the purification, quantitation, and analysis of hormone and receptor mRNAs / Martin L. Adamo ... [et al.] -- Polymerase chain reaction: applications to endocrine research / Alan R. Shuldiner and Riccardo Perfetti -- Detection of mutations in hormone receptor genes / Domenico Accili ... [et al.] -- Techniques to study regulation of gene expression / Fredric E. Wondisford.
(cont)   Transgenic animals as a tool in endocrinology: structure/function studies of peptide hormones employing transgenic mice / John J. Kopchick and Wen Y. Chen -- Use of nonmammalian animal models in endocrine investigation / Ian P. Callard, Lynn Riddiford and Colin G. Scanes -- Proliferation induced by growth factors in mammalian fetal cells / Manuel Benito and Margarita Lorenzo -- Expression of hormones/growth factors and their receptors in early embryogenesis / Flora de Pablo, Gilbert A. Schultz and Susan Heyner.
Hormones
Hormone receptors
Molecular endocrinology