CRISPR-Cas : a laboratory manual /
Doudna, Jennifer A.,editor.
Mali, Prashant,editor.
Includes bibliographical references and index.
1. Overview of CRISPR–Cas9 Biology -- 2. Guide RNAs : A Glimpse at the Sequences that Drive CRISPR–Cas Systems -- 3. Characterization of Cas9–Guide RNA Orthologs -- 4. Large-Scale Single Guide RNA Library Construction and Use for CRISPR–Cas9-Based  Genetic Screens -- 5. Adeno-Associated Virus–Mediated Delivery of CRISPR–Cas Systems for Genome Engineering in Mammalian Cells -- 6. Detecting Single-Nucleotide Substitutions Induced by Genome Editing -- 7. CRISPR–Cas9 Genome Engineering in Saccharomyces cerevisiae Cells -- 8. Cas9-Mediated Genome Engineering in Drosophila melanogaster -- 9. Optimization Strategies for the CRISPR–Cas9 Genome-Editing System -- 10. Editing the Mouse Genome Using the CRISPR–Cas9 System -- 11. Genome Editing in Human Pluripotent Stem Cells -- 12. An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells.
RNA