Supramolecular Structure and Function 10 [electronic resource] /
International School on Biophysics "Supramolecular Structure and Function"Corporate Author
International School on Biophysics "Supramolecular Structure and Function"(10th :2009 :Rovinj, Croatia)
International School on Biophysics "Supramolecular Structure and Function"(10th :2009 :Rovinj, Croatia)
Brnjas-Kraljević, Jasminka.editor.
Pifat-Mrzljak, Greta.editor.
The book is based on lectures presented on the International Summer School on Biophysics held in Croatia in September 2009. The advantage of the School is that it provides advanced training in very broad scope of areas related to biophysics contrary to other similar schools or workshops that are centered mainly on one topic or technique. In this volume, tenth in the row, the papers in the field of biophysics are presented. The topics are biological phenomena from single protein to macromolecular aggregations structure by using variant physical methods (NMR, EPR, FTIR, Mass Spectrometry, etc.). The interrelationship of supramolecular structures and their functions is enlightened by applications of principals of these physical methods in the biophysical and molecular biology context.
Description based upon print version of record.
Preface; Contents; Contributors; Fluorescence Correlation Spectroscopy: Principles and Developments; 1 Introduction; 2 Principles of FCS; 2.1 What Is FCS?; 2.1.1 Fluorescence; 2.1.2 Correlation; 2.1.3 Spectroscopy; 2.2 The Geometrical Factor       ; 2.3 The Signal-to-Noise in FCS; 2.4 The Limitations of FCS; 2.5 Reactions; 2.5.1 Triplet-State; 2.5.2 Unimolecular Reactions; 2.5.3 Bimolecular Reactions; 2.6 Multiple Species; 2.7 Cross-Correlation Spectroscopy; 2.8 Pulsed Interleaved Excitation; 3 Advanced FCS Methods; 3.1 Scanning FCS; 3.2 Two-Focus FCS
3.3 Raster Image Correlation SpectroscopyReferences; Time-Resolved FT-IR Spectroscopy for the Elucidation of Protein Function; 1 Introduction; 2 Time-Resolved FT-IR Techniques; 2.1 Rapid-Scan FT-IR Spectroscopy; 2.2 Step-Scan FT-IR Spectroscopy; 2.2.1 Step-Scan Device; 2.2.2 Step-Scan Timing; 2.2.3 Step-Scan Parameters; 3 Applications: Bacteriorhodopsin; 4 New Developments of the Step-Scan FT-IR Technique; References; Recombinant Membrane Protein Production: Past, Present and Future; 1 Introduction; 2 Membrane Targeting and Insertion; 3 Naturally Abundant Membrane Proteins
4 Expression Systems4.1 Prokaryotic Expression Systems; 4.1.1 Escherichia Coli; 4.1.2 Lactococcus Lactis; 4.1.3 Alternative Bacterial Expression Systems; 4.2 Eukaryotic Expression Systems; 4.2.1 Yeasts; 4.2.2 Insect Cells; 4.2.3 Mammalian Cells; 4.2.4 Alternative Expression Systems; 4.3 Cell-Free Expression; 5 Expression Tools; 6 Host Screening/Strain Selection; 7 Protein Engineering; 8 Homologue Screening; 9 Stress Response Upon Membrane Protein Expression; 10 Rational Engineering of Membrane Protein Expression Through Co-expression of Limiting Factors
11 Strategies to Optimize Functional Expression12 Conclusions; References; Cold Denaturation and Protein Stability; 1 Introduction; 2 Unbiased Cold Denaturation; 3 Alcohols: Denaturants or Structure Stabilizers?; 4 Stability and Aggregation; References; Polyglutamine Diseases and Neurodegeneration: The Example of Ataxin-1; 1 Introduction; 2 Structural Features of Atx1; 2.1 Atx1 Domain Architecture; 2.2 The Structure of the Atx1 AXH Domain; 2.3 Atx1 ULM Functions as a Molecular Switch; 3 Atx1 Functions; 3.1 A Role for Atx1 in Transcriptional Repression; 3.2 RNA Processing
3.3 The Multiple Role of the AXH Motif in Nucleic  Acid- and Protein-Protein Interactions3.4 Towards the Determination of the Atx1 Interactome; 4 Proteasome Functions in SCA1; 5 Conclusions; References; Phase Plate Electron Microscopy; 1 Introduction; 1.1 Defocus Phase Contrast TEM; 1.2 Zernike Phase Contrast TEM; 1.3 Hilbert Differential Contrast TEM; 1.4 Phase Contrast Cryo-TEM; 2 Biological Applications to Two Dimensional Imaging; 2.1 ZPC-TEM Applications in Two Dimensions; 2.2 HDC-TEM Applications in Two Dimensions; 3 Biological Applications to Three Dimensional Imaging
3.1 ZPC-TEM Applications in Three Dimensions
Includes bibliographical references and index.
The book is based on lectures presented on the International Summer School on Biophysics held in Croatia in September 2009. The advantage of the School is that it provides advanced training in very broad scope of areas related to biophysics contrary to other similar schools or workshops that are centered mainly on one topic or technique. In this volume, tenth in the row, the papers in the field of biophysics are presented. The topics are biological phenomena from single protein to macromolecular aggregations structure by using variant physical methods (NMR, EPR, FTIR, Mass Spectrometry, etc.). The interrelationship of supramolecular structures and their functions is enlightened by applications of principals of these physical methods in the biophysical and molecular biology context.
Medicine.
Life sciences.
Biophysics.
Biological physics.
Spectroscopy.
Microscopy.
Bioinformatics.
Cancer research.
Biomedicine, general.
Life Sciences, general.
Biological and Medical Physics, Biophysics.
Spectroscopy and Microscopy.
Bioinformatics.
Cancer Research.