Subcellular Proteomics [electronic resource] : From Cell Deconstruction to System Reconstruction /
Bertrand, Eric.editor.
Faupel, Michel.editor.
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. Recent success stories demonstrated that the combination of subcellular prefractionation methods with proteomic analysis is a very potent approach to simplify complex protein extracts from cells or tissues and to detect low abundance proteins. It also made clear that sophisticated strategies encompassing sample preparation, analytics and validation steps were required to fully exploit the potential of subcellular proteomics. Three sections are devoted to the different levels of subcellular or
Description based upon print version of record.
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. Recent success stories demonstrated that the combination of subcellular prefractionation methods with proteomic analysis is a very potent approach to simplify complex protein extracts from cells or tissues and to detect low abundance proteins. It also made clear that sophisticated strategies encompassing sample preparation, analytics and validation steps were required to fully exploit the potential of subcellular proteomics. Three sections are devoted to the different levels of subcellular or
Includes bibliographical references and index.
Membrane Proteomics -- Keynotes on Membrane Proteomics -- Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics -- Microparticles: A New Tool for Plasma Membrane Sub-cellular Proteomic -- Lipid Raft Proteomics: More than Just Detergent-Resistant Membranes -- Organelle Subproteomes -- Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins -- Synaptosome Proteomics -- Proteomic Analysis of Secreted Exosomes -- Characterization of Supramolecular Protein Complexes -- From Protein—Protein Complexes to Interactomics -- Supramolecular Signalling Complexes in the Nervous System -- Protein Networks and Complexes in Photoreceptor Cilia -- Subcellular Systems Biology -- Systems Biology and the Reconstruction of the Cell: From Molecular Components to Integral Function -- Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy -- Systems Biology of the Endoplasmic Reticulum Stress Response -- Emerging Technologies in Proteomics -- Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis -- Biophotonics Applied to Proteomics -- Differential Epitope Identification of Antibodies Against Intracellular Domains of Alzheimer's Amyloid Precursor Protein Using High Resolution Affinity-mass Spectrometry -- LC-MALDI MS and MS/MS — An Efficient Tool in Proteome Analysis.
Proteins .
Cell biology.
Proteomics.
Biochemistry.
Biophysics.
Biological physics.
Bioinformatics.
Protein Science.
Cell Biology.
Proteomics.
Biochemistry, general.
Biological and Medical Physics, Biophysics.
Bioinformatics.
Sub-cellular biochemistry