PCR-based diagnostics in infectious disease /
Ehrlich, Garth D.
Greenberg, Steven J.
Boston, Mass. ; Oxford : Blackwell Scientific Publications,
1994.
Includes bibliographical references and index.
Establishment of the clinical PCR laboratory -- PCR and its role in clinical diagnostics -- Laboratory facilities, protocols, and operations -- Parameters for maximizing the sensitivity and specificity of PCR -- Methods of RNA amplification -- PCR-based ancillary techniques -- Containment system for PCR amplification and detection -- Commercial PCR kits -- Role of PCR in clinical diagnostics -- Introduction -- Infectious diseases of the immune system -- Infectious diseases of the eye -- Applications of PCR to the diseases of the ears, nose, and throat -- Infectious diseases of the central nervous system.
(cont) Applications of PCR to the diagnosis of infectious diseases affecting the gastrointestinal tract and liver -- Role of PCR in the diagnosis of infectious diseases of the cardiorespiratory system -- PCR-based approaches in the diagnosis of sexually transmitted diseases -- Application of PCR to the diagnosis of infectious diseases of the integument -- Applications of PCR to oral diagnostics -- PCR-based protocols for the detection of specific organisms -- Laboratory protocols for the PCR-based detection of infectious disease agents -- PCR-based strategies for the detection of human enteroviruses -- Detection of hepatitis C virus RNA in liver and plasma by reverse transcriptase PCR using liquid hybridization -- PCR-based strategies for the detection and typing of rotaviruses and other reoviridae.
(cont) PCR-based laboratory methods for the detection of the human retroviridae and hepadnaviridae -- PCR-based methods for the detection of adenoviruses -- PCR-based detection of the herpesviruses and the papillomaviruses -- Diagnosis of rickettsial and ehrlichial diseases using PCR -- Chlamydiales and mycoplasmatales -- DNA amplification systems for Treponema pallidum -- DNA amplification systems for Borrelia burgdorferi -- Detection of mycobacterial DNA using PCR -- Detection of microbial pathogens in the gastrointestinal tract using PCR and gene probe methods -- PCR-based strategies for the detection of H. influenzae and Str. pneumoniae DNA associated with diseases of the ears, nose, and throat -- Detection of Porphyromonas (bacteroides) gingivalis using PCR.
(cont) Detection of Bordetella pertussis using PCR -- Identifying human fungal pathogens using PCR -- Characterization of novel bacterial pathogens by amplification and sequencing of 16S ribosomal RNA genes -- PCR-based approaches for the detection of bacterial resistance.
Communicable diseases
Polymerase chain reaction