Nucleic Acids Hybridization [electronic resource] : Modern Applications /
Buzdin, Anton.editor.
Lukyanov, Sergey.editor.
Watsona ""Crick hybridization of complementary sequences in nucleic acids is one of the most important processes necessary for molecular recognition in vivo, as well as nucleic acid identification and isolation. This book is devoted to a large family of in vitro DNA hybridization-based experimental techniques. A wide spectrum of experimental tasks covered by these approaches includes finding differential sequences in both genomic DNAs and mRNAs, genome walking, multiplex PCR, cDNA library construction starting from minute amount of total RNA, rapid amplification of cDNA 5a (TM) and 3a (TM) end
Description based upon print version of record.
Watsona ""Crick hybridization of complementary sequences in nucleic acids is one of the most important processes necessary for molecular recognition in vivo, as well as nucleic acid identification and isolation. This book is devoted to a large family of in vitro DNA hybridization-based experimental techniques. A wide spectrum of experimental tasks covered by these approaches includes finding differential sequences in both genomic DNAs and mRNAs, genome walking, multiplex PCR, cDNA library construction starting from minute amount of total RNA, rapid amplification of cDNA 5a (TM) and 3a (TM) end
Nucleic Acids Hybridization: Potentials and Limitations -- Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications -- Suppression Subtractive Hybridization -- Stem-Loop Oligonucleotides as Hybridization Probes and Their Practical Use in Molecular Biology and Biomedicine -- Normalization of cDNA Libraries -- Primer Extension Enrichment Reaction (PEER) and Other Methods for Difference Screening -- Subtractive Hybridization with Covalently Modified Oligonucleotides -- Coincidence Cloning: Robust Technique for Isolation of Common Sequences -- DNA Hybridization in Solution for Mutation Detection -- Current Attempts to Improve the Specificity of Nucleic Acids Hybridization -- Concepts on Microarray Design for Genome and Transcriptome Analyses.
Biochemistry.
Cancer research.
Human genetics.
Virology.
Biochemistry, general.
Cancer Research.
Human Genetics.
Virology.