The condensed protocols from Molecular cloning : a laboratory manual /
Sambrook, Joseph.
Russell, David W.(David William),1957-
Sambrook, Joseph.Molecular cloning.
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press,
c2006.
"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
Molecular cloning