Electric Cell-Substrate Impedance Sensing  and Cancer Metastasis [electronic resource] /
Jiang, Wen G.editor.
Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.
Description based upon print version of record.
Electric Cell-Substrate Impedance Sensing and Cancer Metastasis; Preface; Contents; Electric Cell-Substrate Impedance Sensing Concept to Commercialization; 1 The Birth of ECIS; 2 Description of ECIS; 3 Biological Assays; 3.1 The Early Years; 3.2 Response of Cells to External Agents; 3.3 Cancer Research; 3.4 Invasive Electric Fields; 4 Applied BioPhysics; 4.1 The Beginning Years; 4.2 The Importance of Marketing; 4.3 Looking Towards the Future; Reference; Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell Impedance; 1 Introduction
1.1 Protein Kinase C (PKC)1.1.1 Family and Structure; 1.1.2 Regulation of PKC; Phosphorylation; Protein Scaffolding; Downregulation via Dephosphorylation and Proteolysis; 1.1.3 PKC Signal Transduction; 1.2 Cell-Extracellular Matrix (ECM) Structures; 1.2.1 Structural Definition and Mechanical Role; 1.2.2 Regulation of Cell-ECM Dynamic Flux; 1.3 Assessment of PKC Regulation of Focal Adhesion Complexes via Measuring Cell Impedance; 1.3.1 Principles of Cell Impedance Technology; 1.3.2 Assets and Limitations of Measuring Cell Impedance
1.3.3 Investigation of the Crosstalk Between PKC and Focal Adhesion Complexes2 Conclusion; References; ECIS as a Tool in the Study of Metastasis Suppressor Genes: Epithelial Protein Lost In Neoplasm (EPLIN); 1 Introduction; 2 Cancer Metastasis; 2.1 The Metastatic Cascade; 2.2 Metastasis Genes; 3 Epithelial Protein Lost In Neoplasm (EPLIN); 3.1 Discovery/Characterisation; 3.2 Implications in Cancer; 3.3 EPLIN as a Potential Tumour/Metastasis Suppressor; 4 Use of ECIS as a Model System in the Implication of EPLIN as a Metastasis Suppressor; 5 Conclusions and Future Implications; References
Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening1 Introduction; 1.1 Characteristics of Cellular Transformation and Its Effect on Impedance; 1.1.1 Chemical/Membrane Structure; 1.1.2 Morphological Factors; 2 Cellular Transformation; 2.1 ECIS for Measuring Cellular Transformation; 2.2 Stable CXCR2 Transfectants; 2.2.1 Proliferation; 2.2.2 Foci Formation; 2.3 Oral Epithelial Cells; 3 Migration/Metastasis; 3.1 ECIS Measurement of Attachment and Migration; 4 Invasion; 4.1 Classic Invasion Assays
4.2 Invasion Assays with ECIS5 Anticancer Compound Screening; 6 Future of ECIS; 6.1 New Applications of ECIS; 6.2 Microscopy in Tandem with ECIS; 6.3 ECIS and Stem Cell Differentiation; References; Epithelial-Mesenchymal Transition and the Use of ECIS; 1 Introduction; 1.1 Cancer Metastasis; 1.2 Cell Motility; 2 Epithelial-Mesenchymal Transition; 2.1 Mechanisms of EMT; 2.2 EMT Progression; 2.2.1 E-Cadherin; 2.3 Transcription Factors in EMT; 2.4 EMT-Related Factors; 2.4.1 Bone Morphogenetic Protein (BMP7); 2.4.2 Matrix Metalloproteinases (MMPs); 2.4.3 Epithelial Protein Lost In Neoplasm (EPLIN)
3 The Use of ECIS Analysis in the Study of EMT in Relation to Cancer
Includes bibliographical references and index.
Cell based impedance sensing is becoming a new biophysical and cellular technology in cell based analyses. The technology has been used in investigation of cellular growth and death, cell adhesion and migration, cell invasion and cell-cell interactions, cell toxicity, angiogenesis, cell permeability, signal transduction and cellular behaviour under flow conditions. It is a probe free, highly sensitive, and versatile technology platform. Recent development in the technology has also allowed high throughput, automated analyses. It has been widely explored in chemistry, toxicity, cell biology, cancer biology, and other areas of chemistry, medicinal chemistry, life and medical science. Written by experts in the area of cell impedance sensing, including the Nobel Laureate Dr Ivar Giaever, this books covers the background of electric cell-substrate impedance sensing, their applications in cell based investigations, particularly in the area of cancer biology. This book is the first on this technology platform and will be a highly useful reference for molecular and cell biologists, cancer biologists, chemists and biochemists, clinical researchers who work in the areas of cell biology, molecular biology, toxicology, pharmaceutical industry, life science and medical research.
Cancer research.
Medicine.
Molecular biology.
Oncology  .
Cancer Research.
Biomedicine, general.
Molecular Medicine.
Oncology.


Cancer Metastasis - Biology and Treatment