Fibrinolytic Bacterial Enzymes with Thrombolytic Activity [electronic resource] /
Kotb, Essam.author.
Stress, high blood pressure, smoking, pollution, fast foods, overweight, excessive travelling, surgery, less movement are common features in our modern life. These features are risky for blood clotting disorders. According to WHO, over 29% of the total mortalities worldwide are due to thrombosis. By the year, 2020 cardiovascular diseases (CVDs) may cause an estimated 25 million deaths per year, thus antithrombotic therapy is of great interest. The available thrombolytic agents such as urokinase are highly expensive, antigenic, quite unspecific, pyretogenic and hemorrhagenic. Therefore, the production of fibrinolysing enzymes, which rapidly dissolute thrombi within the vascular tree, without the detriments by microorganisms, as described in this book, is the desirable aim of today’s research.
Description based upon print version of record.
Fibrinolytic Bacterial Enzymes with ThrombolyticActivity; Preface; Glossary; Contents; 1 Fibrinolytic Bacterial Enzymes with Thrombolytic Activity; Abstract; 1...Introduction; 1.1 Thrombus Formation; 1.2 Thrombolytic Therapy; 1.3 Sources of Fibrinolytic Enzymes; 1.4 Production of Bacterial Fibrinolytic Enzyme In Vitro; 1.5 Enzyme Purification; 1.6 Biochemical Characteristics of Purified Microbial Fibrinolytic Enzymes; 2...Streptokinase; 2.1 Structure and Mechanism of Action; 2.2 Enhancing Streptokinase; 2.3 Production of Streptokinase; 2.3.1 Producing Microorganisms; 2.3.2 Recombinant Producers
2.3.3 Fermentation Process2.4 Assaying Streptokinase; 2.5 Recovery and Purification; 3...Staphylokinase; 3.1 Structure of Staphylokinase; 3.2 Mechanism of Fibrin Selectivity; 3.3 Pharmacokinetics and Thrombolytic Properties in Patients; 3.4 Immunogenicity; 3.5 PEG-Derivatized Cysteine-Substitution Variants for Single Bolus Administration; 4...Serrapeptase; 5...Nattokinase; 5.1 History of Natto; 5.2 Discovery of Nattokinase; 5.3 Structure and Functions; 5.4 Prolonged Action; 5.5 Thrombolytic Effect In Vivo; 5.6 Benefits for Blood Pressure; 5.7 Preparation of Natto in Japan
5.8 Preparation of Natto in Microbiology Laboratories5.9 Contraindications and Precautions; 5.9.1 Thrombic Embolization; 5.9.2 Interaction with Coumadin; 5.9.3 Intervention with Warfarin; 6...Assessment of Fibrinnolytic Activity; 6.1 Indirect Assays; 6.1.1 Fibrin Plate Method; 6.1.2 Dyed Fibrin Plate Assay; 6.1.3 Plasma Streptokinase Lysis Time (PSLT); 6.1.4 Streptokinase-Activated Lysis Time (SALT); 6.1.5 Nephelometric Method for Recording Lysis Time; 6.1.6 Dilute Blood Clot Lysis Time (DBCLT); 6.1.7 Euglobulin Clot Lysis Time (ECLT); 6.1.8 Effect of Calcium and Decalcifying Agents on Assays
6.2 Esterolytic Assays6.3 Fluorimetric Assays; 7...Hemostasis Screening Tests; 7.1 CBC Assay; 7.2 PT Assay; 7.3 PTT Assay; 7.4 TT Assay; 7.5 Fibrinogen Assays; 7.6 D-dimer Assays; 7.7 BT Assay; 8...Conclusions and Future Research Directions; References
Includes bibliographical references.
Stress, high blood pressure, smoking, pollution, fast foods, overweight, excessive travelling, surgery, less movement are common features in our modern life. These features are risky for blood clotting disorders. According to WHO, over 29% of the total mortalities worldwide are due to thrombosis. By the year, 2020 cardiovascular diseases (CVDs) may cause an estimated 25 million deaths per year, thus antithrombotic therapy is of great interest. The available thrombolytic agents such as urokinase are highly expensive, antigenic, quite unspecific, pyretogenic and hemorrhagenic. Therefore, the production of fibrinolysing enzymes, which rapidly dissolute thrombi within the vascular tree, without the detriments by microorganisms, as described in this book, is the desirable aim of today’s research.
Medical microbiology.
Hematology.
Cardiology.
Human physiology.
Medical Microbiology.
Hematology.
Cardiology.
Human Physiology.
SpringerBriefs in Microbiology