Interplay between Metal Ions and Nucleic Acids [electronic resource] /
Sigel, Astrid.editor.
Sigel, Helmut.editor.
Sigel, Roland KO.editor.
The Metal Ions in Life Sciences series links coordination chemistry and biochemistry in their widest sense and thus increases our understanding of the relationship between the chemistry of metals and life processes. The series reflects the interdisciplinary nature of Biological Inorganic Chemistry and coordinates the efforts of scientists in fields like biochemistry, inorganic and coordination chemistry, molecular and structural biology, enzymology, environmental chemistry, physiology, toxicology, biophysics, pharmacy, and medicine. Consequently, the volumes are an essential source for researchers active in these and related fields as well as teachers preparing courses, e.g., in Bioinorganic Chemistry. MILS-10: Interplay between Metal Ions and Nucleic Acids provides in an authoritative and timely manner in 12 stimulating chapters, written by 24 internationally recognized experts from 8 nations, and supported by nearly 1500 references, about 20 tables, and 125 illustrations, many in color, a most up-to-date view on metal ion-nucleic acid interactions; the characterization of which is covered in solution and in the solid state. The volume concentrates on modern developments encompassing topics in the wide range from G-quadruplexes via DNAzymes, catalysis at the DNA scaffold, and metal-mediated base pairs to peptide nucleic acids (PNAs) being thus of relevance, e.g., for chemistry and nanotechnology but also for molecular biology and (genetic) diagnostics. The Editors Astrid Sigel, Helmut Sigel and Roland K. O. Sigel have long-standing interests in Biological Inorganic Chemistry. They published on metal ion interactions with nucleotides and nucleic acids and on related topics, and edited previously 44 volumes in the series Metal Ions in Biological Systems.
Description based upon print version of record.
""Interplay between MetalIons and Nucleic Acids""; ""Historical Development and Perspectivesof the Series: Metal Ions in Life Sciences*""; ""Metal Ions in Life Sciences .""; ""Preface to Volume 10""; ""Interplay between Metal Ions and Nucleic Acids""; ""Contents""; ""Contributors to Volume 10""; ""Titles of Volumes 1-44 in the Metal Ionsin Biological Systems Series""; ""Contents of Volumes in theMetal Ions in Life Sciences Series""; ""Chapter 1: Characterization of Metal Ion-Nucleic Acid Interactions in Solution""; ""1 Introduction""; ""2 Ligating Sites for Metal Ions in Nucleic Acids""
""3 Metal Ions to Be Considered to Interact with Nucleic Acids""""3.1 Natural Metal Cofactors""; ""3.1.1 Dominance of Mg 2+""; ""3.1.2 Monovalent Ions""; ""3.1.3 Influences of Metal Ions Other than Mg 2+ and K +""; ""3.2 Expanding the Natural Metal Repertoire of Catalytic RNAs and DNAs""; ""3.3 Kinetically Stable Metal Ion Complexes""; ""3.4 Metallated Nucleic Acids for Nanotechnology""; ""4 Structural Characterization of Metal Ion Binding Sites""; ""4.1 Chemical and Biochemical Methods""; ""4.1.1 Metal Ion-Induced Hydrolytic Cleavage""; ""4.1.2 Metal Ion-Induced Radical Cleavage""
""4.1.3 Mutational Approaches to Determine Metal Ligands""""4.1.4 Metal Ion Switch Experiments""; ""4.1.5 Kinetic Isotope Effect""; ""4.2 Spectroscopic Methods""; ""4.2.1 Electron Paramagnetic Resonance""; ""4.2.2 Lanthanide(III) Luminescence""; ""4.2.3 X-ray Absorption Spectroscopy""; ""4.2.4 Vibrational Spectroscopies""; ""4.2.5 Nuclear Magnetic Resonance Methods""; "" 4.2.5.1 Chemical Shift Perturbations:""; "" 4.2.5.2 Paramagnetic Effects:""; "" 4.2.5.3 NOE Crosspeaks to [Co(NH 3) 6 ] 3+ and NH :""; "" 4.2.5.4 Direct Detection of NMR-Active Metal Isotopes:""
""5 Determination of Binding Kinetics""""5.1 Nuclear Magnetic Resonance""; ""5.2 Further Methods""; ""6 Determination of Binding Affinities""; ""6.1 Stoichiometric Methods â€? â€œIon Countingâ€?""; ""6.2 Relative Affinities by Competition Experiments""; ""6.3 Calculating Site-Specific Intrinsic Binding Affinities from NMR Chemical Shifts""; ""7 Effects of Other Factors""; ""7.1 Effect of Anions""; ""7.2 Effect of Buffers""; ""7.3 Effects of Solvent Permittivity and Co-solutes to Mimic Macromolecular Crowding""; ""8 Concluding Remarks""; ""References""
""Chapter 2: Nucleic Acid-Metal Ion Interactions in the Solid State""""1 Introduction""; ""2 Metal-Mononucleotide Complexes""; ""2.1 Structures of Alkali and Alkaline Earth Metal Ion Complexes""; ""2.2 Structures of Transition and Heavy Metal Ion Complexes""; ""2.3 Preferred Metal Binding Sites or Modes""; ""2.4 Factors Affecting Metal Binding Sites""; ""3 Metal-Oligonucleotide Complexes""; ""3.1 RNA Fragments""; ""3.2 DNA Fragments""; ""3.2.1 A-DNA Fragments""; ""3.2.2 B-DNA Fragments""; ""3.2.3 Z-DNA Fragments""; ""3.3 Factors Affecting Metal Binding to Oligonucleotide Duplexes""
""4 Concluding Remarks and Future Directions""
Includes bibliographical references and index.
The Metal Ions in Life Sciences series links coordination chemistry and biochemistry in their widest sense and thus increases our understanding of the relationship between the chemistry of metals and life processes. The series reflects the interdisciplinary nature of Biological Inorganic Chemistry and coordinates the efforts of scientists in fields like biochemistry, inorganic and coordination chemistry, molecular and structural biology, enzymology, environmental chemistry, physiology, toxicology, biophysics, pharmacy, and medicine. Consequently, the volumes are an essential source for researchers active in these and related fields as well as teachers preparing courses, e.g., in Bioinorganic Chemistry. MILS-10: Interplay between Metal Ions and Nucleic Acids provides in an authoritative and timely manner in 12 stimulating chapters, written by 24 internationally recognized experts from 8 nations, and supported by nearly 1500 references, about 20 tables, and 125 illustrations, many in color, a most up-to-date view on metal ion-nucleic acid interactions; the characterization of which is covered in solution and in the solid state. The volume concentrates on modern developments encompassing topics in the wide range from G-quadruplexes via DNAzymes, catalysis at the DNA scaffold, and metal-mediated base pairs to peptide nucleic acids (PNAs) being thus of relevance, e.g., for chemistry and nanotechnology but also for molecular biology and (genetic) diagnostics. The Editors Astrid Sigel, Helmut Sigel and Roland K. O. Sigel have long-standing interests in Biological Inorganic Chemistry. They published on metal ion interactions with nucleotides and nucleic acids and on related topics, and edited previously 44 volumes in the series Metal Ions in Biological Systems.
Nucleic acids.
Analytical chemistry.
Inorganic chemistry.
Biotechnology.
Science education.
Nucleic Acid Chemistry.
Analytical Chemistry.
Inorganic Chemistry.
Biotechnology.
Science, Humanities and Social Sciences, multidisciplinary.
Science Education.
Metal ions in life sciences