Molecular Biology in Plant Pathogenesis and Disease Management [electronic resource] : Microbial Plant Pathogens, Volume 1 /
Narayanasamy, P.author.
Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.
Description based upon print version of record.
CONTENTS; Preface; Acknowledgement; 1 Introduction; 1.1 Molecular Biology as a Research Tool; 1.2 Application of Molecular Techniques; References; 2 Molecular Techniques for Detection of Microbial Pathogens; 2.1 Detection of Microbial Pathogens by Biochemical Techniques; 2.2 Detection of Microbial Pathogens by Immunoassays; 2.3 Detection of Microbial Plant Pathogens by Nucleic Acid-BasedTechniques; Appendix 1: Electrophoretic Characterization of Strains of BacterialPathogen Xanthomonas campestris pv. vesicatoria (Xcv)(Bouzar et al. 1994)
Appendix 2: Detection of Virus-Specific Protein in Infected Leaves bySodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis(SDS-PAGE) (Seifers et al. 1996, 2005)Appendix 3: Indirect ELISA for Assessing Titers of PABs and MABsSpecific to Callalily chlorotic spot virus (CCSV) and Watermelonsilver mottle virus (WSMoV) (Lin et al. 2005); Appendix 4: Detection of Citrus psorosis virus (CPsV) by Direct TissueBlot Immunoassay (DTBIA) (Martin et al. 2002); Appendix 5: Detection of Potato virus Y (PVY) and Cucumber mosaic virus (CMV) in Tobacco by Immunostaining Technique (Ryang et al. 2004)
Appendix 6: Detection of Citrus tristeza virus (CTV) by In Situ Immunoassay (ISIA) (Lin et al. 2000)Appendix 7: Detection of Potyvirus by Western Blot Analysis(Larsen et al. 2003); Appendix 8: Detection of Bacterial Pathogens by Enzyme-LinkedImmunosorbent Assay (ELISA) in Seeds (Lamka et al. 1991; Pataky et al. 2004); Appendix 9: Detection of Ultilago nuda Barley Seeds by DAS-ELISA (Eibel et al. 2005a); Appendix 10: Detection of Plant Viruses by Reverse-Transcription-Polymerase Chain Reaction (RT-PCR) Assay (Huang et al. 2004; Spiegel et al. 2004)
Appendix 11: Detection of Virus (Potato virus Y) by Reverse Transcription - DIAPOPS System (Nicolaisen et al. 2001)Appendix 12: Detection of Grape fan leaf virus (GFLV) in Nematode Vector Xiphinema index by RT-PCR (Finetti-Sialer and Ciancio 2005); Appendix 13: Detection of Potato virus Y by Reverse TranscriptionLoop-Mediated Isothermal Amplification DNA (Nie 2005); Appendix 14: Detection of Fruit Tree Viroids by a Rapid RT-PCR Test(Hassen et al. 2006); Appendix 15: Membrane BIO-PCR Technique for Detection ofBacterial Pathogen (Pseudomonas syringae pv. phaseolicola)(Schaad et al. 2007)
Appendix 16: Detection of Bacterial Pathogens by DNA ArrayTechnology (Fessehaie et al. 2003 Scholberg et al. 2005); Appendix 17: Extraction of Genomic DNA from Fungal Pathogens(Phytophthora spp.) (Lamour and Finley 2006); Appendix 18: Detection of Mycosphaerella graminicola in WheatUsing Reverse Transcription (RT)-PCR (Guo et al. 2005); References; 3 Molecular Variability of Microbial Plant Pathogens; 3.1 Molecular Basis of Variability of Fungal Pathogens; 3.2 Molecular Basis of Variability of Bacterial Pathogens; 3.3 Molecular Basis of Variability of Viral Pathogens
3.4 Molecular Basis of Variability of Viroid Pathogens
Includes bibliographical references and index.
Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.
Plant pathology.
Plant genetics.
Microbiology.
Plant physiology.
Plant Pathology.
Plant Genetics and Genomics.
Microbiology.
Plant Physiology.