Practical capillary electrophoresis /
Weinberger, Robert.
Boston, Mass. ; London : Academic Press,
c1993.
Includes bibliographical references and index.
Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Historical perspective -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family ot techniques -- Basic concepts -- Electrical conduction in fluid solution -- Language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Capillaries -- Capillary zone electrophoresis -- Mobility -- Buffer additives -- Solute-wall interactions -- Bandbroadening -- Applications -- Methods development -- Capillary isoelectric focusing -- Capillary coatings and electroosmotic flow -- Performing a run -- Focusing and mobilization problems -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for CGE -- Applications -- Pulsed-field capillary electrophoresis -- Future of CGE -- Capillary isotachophoresis -- Separation mechanism -- Instrumental aspects -- Buffer selection.
(cont) Advantages and problems with CITP -- Applications -- Electrokinetic capillary chromatography -- Micelles -- Separation mechanism -- Sources of bandbroadening -- Selecting the electrolyte system -- Elution range of MECC -- Alternative surfactant systems -- Applications and methods development -- Cyclodextrins -- Chiral recognition -- Optimization -- Capillary electrochromatography -- Electroosmotic pump -- Bandbroadening -- Applications -- Electrochromatography in perspective -- Injection -- Volumetric constraints on injection size -- Performing an injection and run -- Injection techniques -- Injection problems and solutions -- Alternative injection schemes -- Stacking -- Other enrichment techniques -- Detection -- On-capillary detection -- Detection problem -- Classes of detectors -- Limits of detection -- Bandbroadening -- Absorption detection -- Fluorescence detection -- Indirect detection -- Mass spectrometry -- Other detection techniques -- Fraction collection -- Putting it all together -- Selecting the mode of HPCE -- Requirements for robust separations.
(cont) Realistic compromises -- Linear dynamic range -- Sample preparation -- Quantitative analysis -- Correction factors -- Special topics -- Field effect electroendoosmosis -- Ultrafast separations -- HPCE on a chip -- Multiple capillary systems -- Gradient and programmed systems -- Two-dimensional systems -- Immunologic trace enrichment.
Capillary electrophoresis.