Immunocytochemical methods and protocols /
Javois, Lorette C.
Totowa, N.J. : Humana Press,
c1994.
Includes bibliographical referemces and index.
Antibody preparation -- Overview of antibody use in immunocytochemistry -- Purification of antibodies using ammonium sulfate fractionation or gel filtration -- Purification of antibodies using ion-exchange chromatography -- Purification of antibodies using affinity chromatography -- Purification of antibodies using protein A-sepharose and FPLC -- Conjugation of fluorochromes to antibodies -- Biotinylation of antibodies -- Tissue preparation for light microscopic analyses -- Overview of cell fixation and permeabilization.
Preparation of frozen sections for analysis -- Processing of cell-touch preparations -- Processing of cytological specimens -- Processing of tissue-culture cells -- Processing of tissue specimens -- Light microscopic detection systems -- Overview of fluorophores -- Direct immunofluorescent labeling of cells -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells.
Fluorescent labeling of surface or intracellular antigens in whole-amounts -- Overview of antigen detection through enzymatic activity -- Peroxidase-antiperoxidase (PAP) method -- Avidin-biotin complex (ABC) method -- Labeled avidin binding (LAB) method -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue -- ELISA on attachment-dependent or suspension grown cells -- Use of immunogold with silver enhancement -- Fluorescence-activated cell sorter (FACS) analyses -- Overview of flow cytometry and fluorescent probes for cytometry.
Tissue disaggregation -- Indirect immunofluorescent labeling of viable cells -- Indirect immunofluorescent labeling of fixed cells -- Fluorescent labeling of DNA -- Deparaffinization and processing of pathologic material -- Assay for phagocytosis -- Assay for filamentous actin -- Assay for chemoattractant binding -- Assay for oxidative metabolism -- Colloidal gold detection systems for electron microscopic analyses -- Fixation and embedding -- Preparation of colloidal gold -- Conjugation of colloidal gold to proteins.
Colloidal gold/streptavidin methods -- Pre-embedding labeling methods -- Postembedding labeling methods -- Photography -- Overview of fluorescence photomicrography -- Special applications -- Overview of fluorescence analysis with the confocal microscope -- Overview of laser microbeam applications as related to antibody targeting -- Nucleic acid immunocytochemistry: in situ hybridization to human chromosomes of an alkaline phosphatase-labeled centromeric probe -- Fluorescence in situ hybridization using whole chromosome library probes.
Overview of automated immunostainers -- Overview of immunocytochemical approaches to the differential diagnosis of tumors: diagnostic applications in the clinical laboratory.
Immunocytochemistry