In vitro transcription and translation protocols /
Tymms, Martin J.
Totowa, N.J. : Humana Press,
c1995.
Includes bibliographical references and index.
Transcription in vitro using bacteriophage RNA polymerases / Elaine T. Schenborn -- Subtraction hybridization cDNA libraries / Clifford W. Schweinfest ... [et al.] -- Quantitative measurement of mRNA using the RNase protection assay / Martin J. Tymms -- Assembly and transcription of chromatin templates using RNA polymerase III / Sara J. Felts -- Antisense affinity depletion of RNP particles: application to spliceosomal snRNPs / Benjamin J. Blencowe, Silvia M.L. Barabino -- In vitro mRNA editing using S100 extracts / Jobst Greeve, James Scott.
In vitro transcription assay for probing drug-DNA interactions during active transcription of DNA / Don R. Phillips, Donald M. Crothers -- In vitro reconstitution of progesterone-dependent RNA transcription in nuclear extracts of human breast carcinoma cells / Milan K. Bagchi, Sophia Y. Tsai, Ming-Jer Tsai -- Plastid in vitro transcription / Kai Tiller, Gerhard Link -- Microinjection of in vitro transcribed RNA and antisense oligonucleotides in mouse oocytes and early embryos to study the gain- and loss-of-function of genes / Ismail Kola, Sony Heru Sumarsono.
mRNA translation in Xenopus oocytes / Aldo Ceriotti, Alan Colman -- Xenopus egg extract translation system / Glenn M. Matthews, Alan Colman -- In vitro translation using rabbit reticulocyte lysate / Gregory S. Beckler, David Thompson, Tom Van Oosbree -- In vitro transcription system from BmN cells of the silkworm, Bombyx mori / Hiroshi Sakurai, Susumu Izumi, Shiro Tomino -- Translation using a wheat-germ extract / John F. Van Herwynen, Gregory S. Beckler -- In vitro transcription and translation in a cell-free system from Clostridium tetani / Bettina Andersen-Bechk, Heiner Niemann.
Preparation and use of E. coli S-30 extracts / Scott A. Lesley -- Posttranslational transport and in thylakoido integration of plastid protein / Elisabeth Kruse, Klaus Kloppstech -- Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro / Ruby H.P. Law, Phillip Nagley -- Structure-function studies based on in vitro expression / Martin J. Tymms, Paul J. Hertzog -- [delta]-like plastid transcription factors / Kai Tiller, Gerhard Link -- Preparation of HeLa nuclear extracts / B. Franklin Pugh.
Purification of the human TATA-binding protein, TBP / B. Franklin Pugh -- DNA-binding studies using in vitro synthesized Myb proteins / Robert George Ramsay -- DNase I footprinting using PCR-generated end-labeled DNA probes / Rocco C. Iannello -- Gene isolation by screenig [gamma]gt11 expression libraries with DNA binding-site probes / Bill Kalionis -- Transcriptional activation analysis by the chloramphenicol acetyl transferase (CAT) enzyme assay / David R. Hodge ... [et al.].
Moleuclar genetics
Genetic transcription
Genetic translation