The Eukaryotic Replisome: a Guide to Protein Structure and Function [electronic resource] /
MacNeill, Stuart.editor.
Successful chromosome replication is vital for maintaining the integrity of the genetic material in all forms of cellular life. In humans, there are clear links between chromosome replication defects and genome instability, genetic disease and cancer, making a detailed understanding of the molecular mechanisms of genome duplication vital for future advances in diagnosis and treatment. Inspired by recent exciting breakthroughs in protein structure determination and written by leading experts in the field, The Eukaryotic Replisome: a guide to protein structure and function takes the reader on a guided journey through the intricate molecular machinery of eukaryotic chromosomal DNA replication, from replication origin recognition and the assembly of the pre-replicative complexes in G1 through to the final processing of Okazaki fragments at the end of S-phase. This extensively illustrated book is an invaluable source of information, ideas and inspiration for all those with an interest in chromosome replication, whether from a basic science, translational biology and medical research perspective.
Description based upon print version of record.
The Eukaryotic Replisome: A Guide to Protein Structure and Function; Preface; Contents; Chapter 1: Composition and Dynamics of the Eukaryotic Replisome: A Brief Overview; 1.1 Introduction; 1.2 Replication Origins and the Origin Recognition Complex; 1.3 Formation of the Pre-RC at Origins; 1.4 The Replisome Progression Complex; 1.5 The Replicative Polymerases; 1.6 Sliding Clamp and Clamp Loader Complexes; 1.7 Okazaki Fragment Processing; 1.8 Model Systems for the Studying Eukaryotic Replication; 1.8.1 SV40; 1.8.2 Yeast; 1.8.3 Xenopus; 1.8.4 Archaea; 1.8.5 Other Model Systems; 1.9 Conclusions
ReferencesChapter 2: Evolutionary Diversification of Eukaryotic DNA Replication Machinery; 2.1 Introduction; 2.2 Eukaryotic Diversity; 2.3 Conservation of Replisome Proteins; 2.4 Indispensable Replisome Proteins; 2.5 Replisome Proteins Present in All Eukaryotic Supergroups; 2.6 Replisome Proteins Not Present in All Supergroups; 2.7 A Complex Ancestral Replisome; 2.8 Conclusions; References; Chapter 3: The Origin Recognition Complex: A Biochemical and Structural View; 3.1 Introduction; 3.2 The S. cerevisiae ORC; 3.3 The S. pombe ORC; 3.4 The D. melanogaster ORC; 3.5 The H. sapiens ORC
3.6 Future PerspectivesReferences; Chapter 4: Archaeal Orc1/Cdc6 Proteins; 4.1 Introduction; 4.2 Origins of DNA Replication in the Archaea; 4.3 Orc1/Cdc6 Structure; 4.4 Structures of Orc1/Cdc6s Bound to DNA; 4.5 Beyond Binding Origins - What Do Orc1/Cdc6s do?; References; Chapter 5: Cdt1 and Geminin in DNA Replication Initiation; 5.1 Cdt1 and Geminin: A Functional Preview; 5.1.1 The Multiple Faces of Geminin; 5.1.1.1 Geminin Functions in Replication Licensing; 5.1.1.2 Geminin in the Cell Cycle; 5.1.1.3 Geminin in Cell Differentiation; 5.1.2 The Structure of Geminin
5.1.2.1 The N-Terminal Domain5.1.2.2 The Coiled-Coil Domain; 5.1.3 The Structure of Cdt1; 5.1.3.1 The N-Terminal Domain Is Highly Regulated; 5.1.3.2 The Structurally Conserved Winged Helix Domains; 5.1.3.3 The Recruitment of Cdt1 on Chromatin; 5.1.4 The Cdt1-Geminin Complex; 5.1.4.1 The Primary and Secondary Interfaces; 5.1.4.2 The Tertiary Interface; 5.1.4.3 Conformational Change of the N-Terminal Domain?; 5.1.5 Models for a Cdt1-Geminin Molecular Switch; 5.2 Conclusions; References; Chapter 6: MCM Structure and Mechanics: What We Have Learned from Archaeal MCM; 6.1 Introduction
6.2 Complex Organization: Hexamers and Double Hexamers6.3 Helicase Activity; 6.3.1 Steric Exclusion; 6.3.2 Ploughshare; 6.3.3 LTag Looping Model (or Strand Exclusion); 6.3.4 Rotary Pump; 6.4 Domains and Features of an MCM Subunit; 6.4.1 N Domain; 6.4.2 C Domain; 6.4.2.1 ATP Binding Pocket; 6.4.2.2 Hairpins, Helices and Inserts; 6.4.2.3 Winged Helix Domain; 6.5 Inter- and Intra-Subunit Communication; 6.6 Higher-Order MCM Oligomers; 6.7 Conclusions; References; Chapter 7: The Eukaryotic Mcm2-7 Replicative Helicase; 7.1 Introduction; 7.2 The 'Mcm Problem' and Nonequivalent ATPase Active Sites
7.3 Discovery of Mcm2-7 Helicase Activity and the Mcm2/5 Gate
Includes bibliographical references and index.
Successful chromosome replication is vital for maintaining the integrity of the genetic material in all forms of cellular life. In humans, there are clear links between chromosome replication defects and genome instability, genetic disease and cancer, making a detailed understanding of the molecular mechanisms of genome duplication vital for future advances in diagnosis and treatment. Inspired by recent exciting breakthroughs in protein structure determination and written by leading experts in the field, The Eukaryotic Replisome: a guide to protein structure and function takes the reader on a guided journey through the intricate molecular machinery of eukaryotic chromosomal DNA replication, from replication origin recognition and the assembly of the pre-replicative complexes in G1 through to the final processing of Okazaki fragments at the end of S-phase. This extensively illustrated book is an invaluable source of information, ideas and inspiration for all those with an interest in chromosome replication, whether from a basic science, translational biology and medical research perspective.
Medicine.
Biochemistry.
Proteins .
Nucleic acids.
Cancer research.
Biomedicine, general.
Biochemistry, general.
Protein Science.
Protein Structure.
Nucleic Acid Chemistry.
Cancer Research.
Sub-cellular biochemistry