Reviews in Fluorescence 2015 [electronic resource] /
Geddes, Chris D.editor.
Reviews in Fluorescence 2015, the eighth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year’s progress in fluorescence and its applications, with authoritative reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any research lab or company working in the fluorescence field and related areas.  All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource.
Description based upon print version of record.
Preface; Contents; Chapter 1: Studying Protein Misfolding and Aggregation by Fluorescence Spectroscopy; 1.1 A Brief Introduction to Protein Misfolding and Aggregation; 1.2 Experimental Techniques to Study Protein Aggregation; 1.3 Fluorescence Spectroscopy and Protein Aggregation; 1.4 Fluorescence Techniques to Study Protein Oligomers and Amyloids During Protein Aggregation; 1.4.1 Fluorescence Intensity, Spectrum and Lifetime; 1.4.1.1 Intrinsic Fluorophores; 1.4.1.2 Extrinsic Fluorophores; 1.4.2 Fluorescence Polarization (Anisotropy); 1.4.3 Fluorescence Quenching
1.4.4 Fluorescence Resonance Energy Transfer (FRET)1.4.5 Single-Molecule Fluorescence Studies; 1.4.6 Fluorescence Correlation Spectroscopy (FCS); 1.5 Case Studies from our Laboratory at IISER Mohali; 1.5.1 Lysozyme Aggregation; 1.5.2 Serum Albumin Aggregation; 1.5.3 Conformational Property of kappa-Casein - A Model Intrinsically Disordered Protein; 1.6 Conclusions and Future Directions; References; Chapter 2: Time-Dependent Spectral Shifts in Tryptophan Fluorescence: Bridging Experiments with Molecular Dynamics Simulations; 2.1 Introduction
2.2 Relationship Between Spectral Shifts and Electronic Energy Levels2.3 Effect of the Electric Field on the Electronic Energy Levels; 2.4 Nonlinear Stark Effect; 2.5 Hybrid QM-MD Versus Classical MD; 2.6 Direct-Response Versus Linear-Response Method; 2.7 Separation of Contributions from Different Motions; 2.8 Dielectric Relaxation of Bulk Solvent; 2.9 Relaxation of Water Molecules Near Protein Surface and Inside Protein; 2.10 Separation of Relaxation Modes by Their Time Scales; 2.11 Conclusions; References; Chapter 3: Directional Fluorescence Based on Surface Plasmon-Coupling
3.1 Introduction3.2 Properties of SPCE; 3.2.1 Directional Emission; 3.2.2 Background Suppression; 3.2.3 P-Polarized Emission; 3.2.4 Distance-Determined Coupling; 3.3 The Strategies for Improving SPCE; 3.3.1 Enlarging the Optical Window; 3.3.2 Improving the Applicability and Sensitivity; 3.3.3 Obtaining Controllable Coupling with Spatial Resolution; 3.4 SPCE Optical Imaging; 3.4.1 SPCE Imaging System Based on Microscope Technique; 3.4.2 Point Spread Function of SPCE Microscope; 3.4.3 Present Researches Utilizing Optical Imaging; 3.5 The Applications of SPCE in Analysis; 3.5.1 DNA Sensing
3.5.2 Aptamers-Based Protein Sensing3.5.3 Immunological Detection; 3.5.4 Other Fluorescence Analysis; 3.6 Conclusions; References; Chapter 4: Fluorescence Analysis of Thermoresponsive Polymers; 4.1 Introduction; 4.2 Stimuli-Responsive Polymers; 4.2.1 Thermoresponsive Polymers; 4.2.2 Poly(N-IsoPropylAcrylamide), PNIPAm; 4.3 Polymer Characterisation by Fluorescence; 4.4 Thermoresponsive Polymer Characterization; 4.4.1 Water Sorption; 4.4.2 Physicochemical Parameters by Solvatochromism; 4.4.3 Physicochemical Parameters by Fluorescence
4.4.4 Critical Micelle Concentration (CMC) and Aggregation Measurements
Includes bibliographical references at the end of each chapters and index.
Reviews in Fluorescence 2015, the eighth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year’s progress in fluorescence and its applications, with authoritative reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any research lab or company working in the fluorescence field and related areas.  All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource.
Medicine.
Biotechnology.
Analytical chemistry.
Physical chemistry.
Biomedicine, general.
Biotechnology.
Analytical Chemistry.
Physical Chemistry.