Calcium Signaling [electronic resource] /
Islam, Md. Shahidul.editor.
This volume contains a unique selection of chapters covering a wealth of contemporary topics in this ubiquitous and diverse system of cell signaling. It offers much more than the accessibility and authority of a primary text book, exploring topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative.
Description based upon print version of record.
Calcium Signaling; Preface; Contents; Chapter 1: The Regulation of a Cell's Ca 2+ Signaling Toolkit: The Ca 2+ Homeostasome; Components of the Calcium Signaling Toolkit with a Special Focus on Ca 2+ -Binding Proteins (CaBP) Classified as "Ca 2+ Buffers"; General Considerations; Ca 2+ Buffers/Ca 2+ Sensors Are Components of the Ca 2+ Homeostasome; Adaptation/Compensation/Homeostatic Mechanisms; General Considerations; Temporally Overlapping Activities of Ca 2+ Signaling Toolkit Components Impede the Precise Determination of Their Contribution to Intra- and Intercellular Ca 2+ Signaling
Cellular Modifications Caused by Ablation/Blocking of a Ca 2+ Signaling Toolkit ComponentThe Ca 2+ Homeostasome; The Cellular Ca 2+ Homeostasome; The "Global" Ca 2+ Homeostasome; Conclusions, Hypotheses and Outlook; Note added in proof; References; Chapter 2: Methods to Detect Ca 2+ in Living Cells; Methods to Measure [Ca 2+ ] i Before the Era of Fluorescent Ca 2+ Indicators; Fluorescent Ca 2+ Indicators; How Does One Decide on Which Ca 2+ Indicator to Use?; How Does One Introduce Indicators into Cells?; Equipment Overview; Calibration; Future Directions; References
Chapter 3: Development and Optimization of FLIPR High Throughput Calcium Assays for Ion Channels and GPCRsIntroduction; Calcium - A Universal Signaling Molecule; GPCRs; Ca 2+ -Permeable Ion Channels; Calcium Assays; Chemical Ca 2+ Indicator Dyes; Ratiometric Dyes; Quin-2; Fura-2; Bis-Fura-2; Fura-4F, Fura-5F, Fura-6F and Fura-FF; Fura-PE3 (Fura-2 LeakRes); FFP18; Fura-Red; Mag-Fura-2; Indo-1; Indo-1-PE3 (Indo-1 LeakRes); Mag-Indo-1; Single Wavelength Dyes; Fluo-3; Fluo-4; Mag-Fluo-4; Fluo-5F, Fluo-5Cl, Fluo-5N and Fluo-4FF; Fluo-8, Fluo-8H and Fluo-8L
Rhod-2, X-Rhod-1 and Low Affinity DerivativesCalcium-Green-1 and Calcium-Green-2 Indicators; Oregon Green 488 BAPTA Indicators; Calcium Crimson, Calcium Ruby and Calcium Orange; Dye Loading; AM Ester Loading Technique; No-Wash Extracellular Quenchers; Problems with AM Ester Loading; FLIPR; Excitation Optics; Emission Optics; Emission Filters; Fluidics; Plate Stage; Software and Optional Accessories; Materials and Methods; Design and Optimization of FLIPR Assays; Agonists; Materials; Methods; PDL Coating; Cell Plating; Reagent Plate Preparation; Dye Preparation; Loading Cells with Dye
Assay on FLIPR TETRA (Table  3.4)Applications; Examples; a 1B Adrenoreceptor Transiently Expressed in Cos-1 Cells (Figs.  3.2 and 3.3); Transfection; Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; PAR2 Endogenously Expressed in HEK293 Cells (Fig.  3.4); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; a  7 nAChR Endogenously Expressed in SH-SY5Y Cells (Fig  3.5); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; References; Chapter 4: Two-Photon Calcium Imaging in the Intact Brain; Introduction; Two-Photon Microscopy for In Vivo Fluorescence Imaging
Fluorescent Calcium Indicators
Includes bibliographical references and index.
This volume contains a unique selection of chapters covering a wealth of contemporary topics in this ubiquitous and diverse system of cell signaling. It offers much more than the accessibility and authority of a primary text book, exploring topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative.
Medicine.
Neurosciences.
Proteins .
Cell biology.
Cell physiology.
Biomedicine, general.
Neurosciences.
Receptors.
Cell Biology.
Cell Physiology.
Advances in experimental medicine and biology