Immobilized affinity ligand techniques /
Hermanson, Greg T.
Mallia, A. Krishna.
Smith, Paul K.(Paul Keith)
San Diego, Calif. ; London : Academic Press,
c1992.
Includes bibliographical references (p. 421-429) and index.
Matrix -- General considerations -- Natural supports -- Synthetic supports -- Activation methods -- General considerations -- Procedures -- Immobilization of ligands -- Small ligands -- General protein immobilization -- Immobilization of enzymes -- Peptide antigens, antibodies, and immunoglobulin binding proteins -- Immobilization of lectins -- Immobilized nucleic acids -- Thiphilic adsorbents -- Immobilized disulfide reductants -- Techniques of the trade -- Measurement of activation level and immobilized ligand density -- Affinity techniques -- Selected applications -- Purification -- Scavenging -- Catalysis and modification -- Analytical applications of affinity ligands.
Ligand binding (Biochemistry)
Immobilized ligands (Biochemistry)
Immunoadsorption.
Affinity chromatography.