MicroRNAs as Tools in Biopharmaceutical Production [electronic resource] /
Barron, Niall.editor.
MicroRNAs are small, non-coding RNA molecules that interact with target mRNAs to modulate their translation. They are now recognized as widespread and critical regulators of gene expression in the cell. This book is aimed at both industry- and academic-based researchers in the Biopharmaceutical production space who wish to learn more about these fascinating molecules. It provides an overview of what is currently known about microRNAs including: ·      a description of their biogenesis and mode of action ·      how they can be detected and measured ·      methods for predicting their target genes ·      how we can manipulate their levels in the cell ·      what lessons can be learned from their behaviour in cancer cells Most importantly this volume addresses how microRNAs might be utilised in the bioprocess setting to: ·      gain new understanding of CHO cell biology ·      design microRNAs expression engineering strategies to influence cell characteristics such as proliferation rate or recombinant protein productivity ·      predict phenotypic outcomes in cell line development projects.
Description based upon print version of record.
Preface; Contents; List of Contributors; Chapter 1 A General Introduction to MicroRNAs,Their Investigation and Exploitation inCHO Cell Lines; 1.1 Introduction; 1.2 Biogenesis and Evolution; 1.3 Informative Tools to Predict Targets of MicroRNAs; 1.3.1 Target Binding and Specificity; 1.3.2 MicroRNA Networks; 1.4 Parameters that Govern Repression; 1.4.1 MicroRNA Stability and Abundance; 1.4.2 RNA Editing; 1.4.3 Control of MicroRNA Expression; 1.5 Cellular Effects of MicroRNA Manipulation; 1.5.1 Signalling Pathways; 1.5.2 MicroRNA and Stress; 1.5.3 MicroRNA and Culture Conditions
1.6 Validation of MicroRNAs Target Predictions in Bioprocessing Applications1.7 Conclusion and Perspectives; References; Chapter2 Biogenesis of Mammalian miRNA; 2.1 Introduction; 2.2 Principles of MicroRNA Biogenesis; 2.3 Nuclear Processing by Drosha; 2.4 Cytosolic Processing by Dicer; 2.5 Targeting is Influenced by The 5 End of The MicroRNA; 2.6 RISC Assembly; 2.7 MicroRNA Repress Protein Translation; 2.8 Target Regulation by Altering Transcript Stability; 2.9 Summary; References; Chapter3 Target Prediction Algorithms and Bioinformatics Resources for miRNA Studies; 3.1 Introduction
3.2 Principles of MicroRNA Target Prediction3.2.1 MicroRNA Seed Region Base Pairing; 3.2.2 mRNA Target Site Location and Abundance; 3.2.3 Cross Species Conservation; 3.2.4 Thermodynamic Calculations; 3.3 miRNA Target Prediction Algorithms; 3.3.1 miRanda; 3.3.2 PicTar; 3.3.3 Diana-microT; 3.3.4 TargetScan/TargetScanS; 3.3.5 PITA; 3.3.6 RNAhybrid; 3.3.7 RNA22; 3.3.8 mirWalk; 3.3.9 Machine Learning; 3.3.10 Combining in-silico Prediction with Experimental Data; 3.4 Publicly Available miRNA Data Repositories; 3.4.1 miRbase; 3.4.2 microRNA.org; 3.4.3 MicroCosm; 3.4.4 Tarbase; 3.4.5 MirTarBase
3.4.6 StarBase3.4.7 miRecords; 3.4.8 miR2disease; 3.5 miRNA Prediction Algorithm Performance; 3.6 Utilisation of miRNA Target Prediction in CHO Research; 3.7 Conclusions; References; Chapter4 The CHO miRNA Transcriptome; 4.1 MicroRNA Expression and Cellular Phenotypesin Mammalian Cells; 4.2 MicroRNAs in the Context of Biopharmaceutical Production: EngimiRs and Biomarkers; 4.3 Breaking the Ground for CHO-Specific miRNA Profiling; 4.4 The CHO miRNome; 4.4.1 Correlation of miRNA Expression with Cellular State; 4.4.2 Linking miRNA Expression and Cellular Proliferationin CHO Cells
4.4.3 ApoptomiRs---miRNAs in Control of Survival and Deathof CHO Cells4.4.4 miRNA Expression Signatures That Support Recombinant Protein Production; 4.5 Perspectives on the Use of miRNA Tools in Biopharmaceutics; References; Chapter5 MicroRNAs as Engineering Targets; 5.1 Introduction; 5.1.1 Potential Role of MicroRNAs in Improving CHO Cells Suitable for Large-Scale Production of Biologicals; 5.1.2 Current Strategies for CHO Cell Engineering; 5.2 Applications of MicroRNA for Pathways Engineering in CHO Cells; 5.2.1 Engineering of Growth and Growth Arrest
5.2.2 Engineering of Apoptosis-Resistant Cell Lines to Increase IVC
Includes bibliographical references and index.
MicroRNAs are small, non-coding RNA molecules that interact with target mRNAs to modulate their translation. They are now recognized as widespread and critical regulators of gene expression in the cell. This book is aimed at both industry- and academic-based researchers in the Biopharmaceutical production space who wish to learn more about these fascinating molecules. It provides an overview of what is currently known about microRNAs including: ·      a description of their biogenesis and mode of action ·      how they can be detected and measured ·      methods for predicting their target genes ·      how we can manipulate their levels in the cell ·      what lessons can be learned from their behaviour in cancer cells Most importantly this volume addresses how microRNAs might be utilised in the bioprocess setting to: ·      gain new understanding of CHO cell biology ·      design microRNAs expression engineering strategies to influence cell characteristics such as proliferation rate or recombinant protein productivity ·      predict phenotypic outcomes in cell line development projects.
Pharmaceutical technology.
Genetic engineering.
Animal genetics.
Pharmaceutical Sciences/Technology.
Genetic Engineering.
Animal Genetics and Genomics.