Title: Selenium or selenium substituted hydroxyapatite and egg shell membrane composites for bone and wound healing
Funder: The Academy of Medical Sciences

Abstract: Eggshell membrane (ESM) is a functional equivalent ECM in avian egg during its development. It has antibacterial and antimicrobial activities to resist bacterial invasion [1]. Selenium (Se) is a common trace element in the body and is important to healthy nutrition, especially in the formation of selenoproteins [2]. Se has been suggested to have anticancer effects as well [3]. Se compounds and hydroxyapatite (HA) containing Se have shown favourable antibacterial properties [4,5]. HA comprises the inorganic part of natural hard tissues. Its excellent bioactivity and osteoconductivity led to potential use in hard-tissue substitutes and regeneratives [6]. An ideal wound dressing and bone substitute can both promote wound/fracture healing and prevent bacterial infection. Through this visit we will report a potential dressing and a new biomaterial prepared by incorporating an optimized concentration of selenium ion and selenium containing HA, into the microfibers of a natural chicken ESM and on its surface, respectively. ESM has adsorption properties for the removal of selenium (Se), that increased by the chemical treatment of this adsorbent by HNO3 and NaOH, as studied previously [7]. This ESM will be used as an adsorbent membrane for Se ions. Selenium substituted hydroxyapatite (SeHA) agglomerates with optimal percentage of Se as we previously reported [8] will be prepared on the sides of an egg shell membrane, relevant to the formation process of bone. Both the morphology and crystal structure of the agglomerates will be studied under certain temperature and pH by varying the holding time. To obtain the optimal cytocompatible Se/SeHA-ESM concentrations, cellular attachment and MTT assay will be performed with different concentrations of Se/SeHA-ESM on the human fibroblast and osteoblast cell lines (which are not available in my country). The morphology of the Se/SeHA-ESM was confirmed by SEM, TEM, XRD and FT-IR. The synthesized Se/SeHA-ESM will be further tested for antibacterial activity against Escherichia coli and Staphylococcus aureus by disc diffusion method and bacterial suspension assay. Objectives:the ultimate goal of the proposed project is to come up with an appropriate cost effective technological package and related procedures for the synthesis, characterization and optimization of the new selenium/SeHA-ESM composites compatible with the requirements of tissue regeneration. Plan&Methodology:Stage A:preparation and characterization of Se-ESM and SeHA-ESM composites. Synthesis of SeHA nanopowders (2%Se) by the aqueous co precipitation method. Characterization of the prepared composites by Using the available provided requirements and devices in the visit institute: SEM, TEM, XRD and FT-IR spectroscopy, and Hg-Porosimeter. Stage B:antibacterial activity. Stage C:in vitro study of the composites using fibroblast and osteoblast cell lines. Determination of cell viability using MTT assay. Study the biodegradability and cytotoxicity of scaffold. References:1-Ahlborn G, Sheldon BW (2005). Poult Sci 84:1935-1941. 2-Andrews PJD, Avenell A, etal (2011). BMJ. 342. 3-Clark LC, Combs G FJr, etal (1996). JAMA 276:1957-1963. 4-Tran PL, Hammond AA, etal (2009). Appl Environ Microb 75:3586-3592. 5-Aksakal B, DemirelM, et al (2018). Biomed Eng-Biomed Tech 1-10. 6-Kim HW, Noh YJ, etal (2002). Biomater 23:4113-4121. 7-Mafu LD, Msagati TAM, etal (2014). 8-Korowash SI, Burdzinska A, etal (2017). Interceram 66:244-252.


Research activity codes: 1.1 NORMAL BIOLOGICAL DEVELOPMENT AND FUNCTIONING;2.1 BIOLOGICAL AND ENDOGENOUS FACTORS
Health categories: MUSCULOSKELETAL;INJURIES AND ACCIDENTS