NCube/papyrus huggingface dataset:
Étude de l’évolution des aptitudes physiques durant la réadaptation fonctionnelle intensive (RFI) chez les blessés médullaires traumatiques

Optimal recovery of range of motion (ROM) and strength are critical objectives of intensive rehabilitation following spinal cord injury (SCI). The aim of this study was to characterize the evolution of ROM and strength at the upper limb and analyze their relationship with functional independence during intensive rehabilitation. Data ROM, strength, spasticity, secondary impairments, functional independence) from 197 participants with SCI were collected at admission and discharge from intensive rehabilitation and were analyzed with descriptive statistics and multivariate analyses (regressions, ANOVA). Specific association analyses were used to determine the level of association between ROM, strength and independent variables such as pain, spasticity and secondary impairments. Lesser deficit in ROM was observed in participants with paraplegia compared to those with tetraplegia. The deficit in individuals with tetraplegia was larger to an extent that most of them did not reach normality in ROM at shoulder joint. Surprisingly, individuals with less severe injury according to the American Spinal Injury Association classification (ASIA D) showed larger deficit of ROM than expected. Joint pain was related to reduce ROM, particularly in participants with ASIA D lesion. Values of muscle strength in individuals with more severe tetraplegia (ASIA ABC) are lower than the ones observed in individuals with less severe injury (ASIA D). Overall, strength and ROM were correlated, except for medial rotation movement. ROM, strength, and secondary impairments are the major determinants of functional independence. In conclusion, shoulder joint was the most affected joint of the upper limb, and, despite of significant gains of ROM, abnormalities were greater than expected. Strength and some secondary impairment were related in loss of ROM and functional independence throughout rehabilitation. More study will be needed to understand the medial rotation movement at the shoulder that seems to evolve differently from other movements, and to determine whether or not changes observed during rehabilitation are maintained over time.

Keyphrases: rehabilitation, Spinal cord injury, range of motion, muscle strength, functional independence, upper limbs
Anatomo-functional magnetic resonance imaging of the spinal cord and its application to the characterization of spinal lesions in cats

Spinal cord injury has a significant impact on quality of life since it can lead to motor (paralysis) and sensory deficits. These deficits evolve in time as reorganisation of the central nervous system occurs, involving physiological and neurochemical mechanisms that are still not fully understood. Given that both the severity of the deficit and the successful rehabilitation process depend on the anatomical pathways that have been altered in the spinal cord, it may be of great interest to assess white matter integrity after a spinal lesion and to evaluate quantitatively the functional state of spinal neurons. The great potential of magnetic resonance imaging (MRI) lies in its ability to investigate both anatomical and functional properties of the central nervous system non invasively. To address the problem of spinal cord injury, this project aimed to evaluate the benefits of diffusion-weighted MRI to assess the integrity of white matter axons that remain after spinal cord injury. The second objective was to evaluate to what extent functional MRI can measure the activity of neurons in the spinal cord. Although widely applied to the brain, diffusion-weighted MRI and functional MRI of the spinal cord are not straightforward. Various issues arise from the small cross-section width of the cord, the presence of cardiac and respiratory motions, and from magnetic field inhomogeneities in the spinal region. The main purpose of the present thesis was therefore to develop methodologies to circumvent these issues. This development notably focused on the optimization of acquisition parameters to image anatomical, diffusion-weighted and functional data in cats and humans at 3T using standard coils and pulse sequences. Moreover, various strategies to correct for susceptibility-induced distortions were investigated and the sensitivity and specificity in spinal cord functional MRI was studied. As a result, acquisition of high spatial and angular diffusion-weighted images and evaluation of the integrity of specific spinal pathways following spinal cord injury was achieved. Moreover, functional activations in the spinal cord of anaesthetized cats was detected. Although encouraging, these results highlight the need for further technical and methodological development in the near-future. Being able to develop a reliable neuroimaging tool for confirming clinical parameters would improve diagnostic and prognosis. It would also enable to monitor the effect of various therapeutic strategies. This would certainly bring hope to a large number of people suffering from trauma and neurodegenerative diseases such as spinal cord injury, tumours, multiple sclerosis and amyotrophic lateral sclerosis.

Keyphrases: Animal, Magnetic Resonance Imaging, Spinal Cord, Diffusion tensor imaging, Functional MRI, Artifact, Animal, Injury
Modulation de l’expression du gène CFTR par le produit du gène FIC1 responsable de la
cholestase familiale intra-hépatique progressive de type 1 :
Identification des mécanismes moléculaires impliqués

Human Progressive Familial Intrahepatic Cholestasis type 1 (PFIC1) is a rare genetic disease provoked by mutations inside the ATP8B1 gene resulting in a general loss of bile acids secretion. An episodic and less severe syndrome called Benign Recurrent Intrahepatic Cholestasis (BRIC) have also been associated with mutations in this gene. PFIC1 patients are suffering from many extra-hepatic manifestations. Some of these manifestations are common to Cystic Fibrosis (CF) patients, carrying mutations in CFTR gene. Moreover, expression of CFTR is decreased for some PFIC1 patients. This study was carried out to define the role of ATP8B1 in the modulation of CFTR gene expression and protein function. A first approach was to identify both gene expression and protein synthesis among various cell lines. Then, we developed a second approach based on in silico analysis of structure and function of ATP8B1 to construct a 2D model of the protein. This approach was correlated with the localization of known mutations of ATP8B1. This analysis showed two possible protein maturation sites, a rich phosphorylation domain and a nuclear receptor interacting domain. The cleavage of the 180 kDa peptide generates a 145kDa (ATPase) and a second cleavage produces a 90 kDa, all identified with a specific antibody directed toward the C-Terminal region of the protein. The 90 kDa peptide should be readdressed to the nucleus after myristoylation to interact with nuclear receptors and transcription factors. This analysis was completed by an interactomic approach which has shown a possible interaction between CFTR and ATP8B1 proteins either directly or mediated by a linker, PDZK1. The last part of this work was dedicated to assess the role of ATP8B1 on the activity of CFTR using two cell lines expressing two different mutated ATP8B1 genes. From all these results, we concluded that ATP8B1 is probably involved in the regulation of CFTR gene expression and CFTR maturation and function. We therefore propose a schematic representation of ATP8B1 synthesis and maturation associated with its putative biological functions in the cell.

Keyphrases: Foie, Approche in silico, Cholestasis, Byler disease, Cystic fibrosis, Liver, Gene regulation, In silico approach, Protein annotation, Protein interactions
Régulation des chaperons de la présentation antigénique par ubiquitination

The invariant chain, which form a nonameric complex with the classical MHC class II molecules. HLA-DM and HLA-DO (non-classical class II molecules) are involved in the presentation of antigens to T lymphocytes. The chaperons molecules of the antigenic presentation can modulate the capacity of the cells to present antigens. The transcriptional regulation of the chaperons and all of the other molecules linked to the MHC is assured by the CIITA transactivator. Little is know of the post-transcriptional mechanisms, other than the fact that HLA-DR molecule can be down-regulated by ubiquitination due to E3 ubiquitin ligase MARCH1. MARCH1 is induce by interleukin-10 in monocytes. The goal of this project is to figure out if ubiquitination by MARCH1 can also regulate the expression of the antigenic presentation chaperons. The experiences were performed in the context of co-transfections in HEK293T cells and the expression of the diverses molecules was evaluated by cell stainings and FACS analysis. The p33 isoform of the invariant chain was found to be down-regulated and degraded in the presence of MARCH1. The invariant chain cytoplasmic tail is not completely essential to this phenomenon; a non-identified molecule, associated with Ii, is probably ubiquitinated by MARCH1 and is then down-regulated, together with Ii. It was shown tha CXCR2, a reeptor involved with the invariant chain and CD44 in the reception of the MIF signal,  is not that molecule. HLA-DO can ben targetd by MARCH1 but this does not seem to be a general phenomenon; the expression of the DO/DM complexes remaning unaffected even with the interaction of those complexes with MARCH1. Therefore, a certain protection seem to be provided by HLA-DM to HLA-DO. The expression of HLA-DM itself is not affected by the presence of MARCH1. However, it was not cleary demonstrated if MARCH1 can modify DM. Some results obtained with a cytoplasmic tail of DM comprising an additional lysine suggest that there is a possibility that MARCH1 interact with DM. Generally, the work presented here show that ubiquitination by MARCH1 is involved in post-transcriptionnal regulation of the p33 isoform of the invariant chain but not in the regulation of HLA-DO and HLA-DM.

Keyphrases: HLA-DM, HLA-DO, MARCH1, MARCH, HLA, Invariant chain (Ii), Non-classical class II molecules, Classical class II MHC molecules
Études sur le rôle d’IL-18 dans l’immunopathogénèse du SIDA

HIV-1, the causative agent of AIDS, induces a deregulated production of several immunologically important cytokines in the infected persons. One of these cytokines is IL-18: a powerful proinflammatory cytokine that can regulate both innate and adaptive immune responses. In vivo, its activity is tightly regulated by IL-18 Binding Protein (IL-18BP), another cytokine that specifically binds and neutralizes IL-18 with high affinity. Previous studies have shown that IL-18 concentrations are significantly increased in the circulation of HIV-infected AIDS patients compared to those in healthy people. However, it is not yet clear how the increased levels of this cytokine affect the development of AIDS in HIV infected persons. Furthermore, little is known concerning the production of IL-18 antagonist (IL-18BP) in these patients. These issues were addressed in the studies presented in this thesis. We measured levels of IL-18 and IL-18BP in the sera of HIV-infected patients by using commercial ELISA kits and compared them with the values obtained from a similar number of healthy HIV-seronegative persons. We also determined the absolute and total number of different NK cell subsets and NK cell activity in the peripheral blood mononuclear cells (PBMC) of these individuals. Finally we determined the effects of recombinant human IL-18 as well as of IL-18-rich sera from AIDS patients on cytolytic activity and survival of human NK cells. Our results show that sera from HIV- infected patients contain up to 3 fold higher levels of IL-18 compared to the sera from healthy people. However, levels of IL-18BP were lower in the infected individuals compared to the healthy ones. Consequently, IL-18/IL-18BP ratio is increased in the patients resulting in a further increase in the concentrations of biologically active IL-18 in the circulation of these patients. Our results show that the concentrations of IL-18 correlated inversely with NK cell numbers as well as with their cytolytic activity in the infected persons. These results suggested the involvement of IL-18 in the disappearance of NK cells that prompted us to determine the potential cytocidal effects of this cytokine on human NK cells. The results from our in vitro experiments show that recombinant human IL-18 and IL-18-rich sera from AIDS patients caused apoptosis in a human NK cell line as well as in primary human NK cells. Anti-FasL antagonist antibodies inhibited this cell death.  In a series of experiments, we found that IL-18 enhances expression of FasL but does not affect the expression of Fas on human NK cells. In vitro IL-18 also stimulated transcription from human FasL promoter. Furthermore, the cytokine also enhanced susceptibility of NK cells to Fas-mediated death, as it decreased the expression of an anti-apoptotic protein Bcl-XL. Our study shows that enhanced IL-18 bioactivity in HIV-infected patients may contribute to the pathogenesis of AIDS by disrupting NK cell homoeostasis.

Keyphrases: HIV, HIV, AIDS, NK Cell, IL-18, IL-18, IL-18 BP, IL-18 BP, FAS, FAS, FASL, FASL
Alternatives à l'assainissement centralisé dans les pays en développement: le cas des zones périurbaines du Vietnam

In the context of high urbanization in Southeast Asia, peri-urban areas suffer increasing environmental pressures and lack access to funding and environmental infrastructures. The thesis focuses on strategies and financing mechanisms that will help reduce the gap between supply and demand for sanitation. The thesis tries to answer the following question: In the context of suburban Hanoi, how does one draw upon local resources to improve the access to and sustainability of sanitation? The case study is a pilot community wastewater management project in the suburbs of Hanoi. The thesis analyzes the demand for sanitation and its impacts on the sustainability of the project in the home, village and region. Two main hypotheses are set forth. First, national resources and local users should additionally fund sanitation infrastructures. Second, there is a need to invest further in activities that enhance the sustainability of sanitation, like education and promotion, which will in turn encourage local contribution. The thesis updates knowledge in two important areas related to sanitation: the institutional framework, which governs the financing of sanitation, and the costs and benefits of alternative sanitation in Vietnam. At the onset of this research, both subjects lacked any significant literature. Despite weak local capacities, it was discovered that the real demand for sanitation happened to be much greater than that found in previous documentation. The research concludes that government agencies could count on a greater contribution from communities if efforts are directed towards education and promotion, on top of conferring greater responsibilities to local populations, and opting for simpler technologies.

Keyphrases: Sanitation, Vietnam, Vietnam, Participation, Participation, Alternative technologies, Community-driven, Financing, Demand
Évaluation de l'intégration de l'énergie de fonctionnement dans la pratique patrimoniale au Québec

At the beginning of the 21st century, conservation of heritage assets is facing many challenges in regard to problems related to supply and consumption of energy, to demography and to urbanization. Further, the perception of heritage assets has changed considerably the reasons leading to ownership of a heritage building (chapter 1).  With the emphasis on energy today, particularly related to the energy consumed by buildings (chapter 2), how do the issues related to heritage conservation interface with energy use and production. If the principles of sustainable development (chapter 3) and conservation of heritage assets combined with effective management of energy needs seem obvious, how is that reflected in reality? How do the professionals of heritage assets integrate the elements of energy consumption in heritage buildings? How do they integrate renewable energy installations and their use in heritage buildings.  To find some answers to the reality of maintaining Quebec heritage, this thesis is limited to professionals with training in architecture (chapter 4). It tries to assess to what extent the laws and regulations consider energy related issues (chapter 5) and tries to define the spirit by which the professionals in the conservation of heritage buildings approach questions related to energy  consumption and the use of renewable energy (chapter 6).

Keyphrases: Heritage, Energy, Energy functioning, Sustainable development, Sustainable heritage, Conservation of energy
Intégration du concept de capacité de support d’un plan d’eau aux apports
en phosphore à l’aménagement du territoire au Québec : Réalité ou utopie?

This study presents a literature review of current carrying capacity models developed in Ontario and Quebec (Canada) for the estimation of phosphorus (P) loading in lakes. Another objective is to evaluate their applications to land and watershed management practices in Quebec. A comparison of theoretical and empirical models, combined with the observations of keys stakeholders, confirms the importance of calibrating models regionally. The analysis further reveals that empirical models estimate more accurately P concentrations in lakes. However, the use of traditional mass balance approaches is still a necessity for the estimation of different P sources in the watershed. These models can support decision makers and land managers in Quebec in the application of more restrictive measures in lakes where the carrying capacity is currently exceeded. In Quebec, the use of the models and their implementation in the legislation will be a municipal responsibility. Many urbanism tools already allow this kind of intervention (zoning, « outils à caractère discrétionnaire »). But, model use alone will not be sufficient to justify a total interdiction of land development in a watershed or to impose a strict limit to house and cottage development.

Keyphrases: Carrying capacity, Phosphorus export, Lakes, Watershed management, Mass balance model
Entretien avec Cécile Laborde

Cécile Laborde has developed the project of a critical republicanism on the basis of a dialogue between republican normative theory and critical social theory. We offer here a presentation and a discussion of the main orientations of that project.

Keyphrases: Cécile Laborde
Le comportement informationnel des jeunes adultes québécois en matière de santé sexuelle

Over the pasts few years, statistics have indicated an exponential growth in the incidence of some sexually transmitted diseases among young adults. Certain studies suggest less-than-responsible sexual health behaviours among this population, despite readily available information, from diverse sources, on the consequences of such behaviours. Furthermore, the sexual health information behaviour of this population has not yet been well documented. This study focuses on the sexual health information behaviour of a group of young Quebec adults. More specifically, it answers the following four research questions: (1) What are the problematic situations faced by young adults in matters of sexual health?; (2) What are the information needs arising from these problematic situations?; (3) What are the information search processes and sources used to answer these information needs? and (4) Once found, how was this information used? This descriptive research used a qualitative approach. The Université de Montréal was chosen as the research environment for two reasons: it is a cognitively rich milieu that offers on-site access to sexual health resources. Eight young adults aged from 18 to 25 participated in an in-depth interview using the critical incident technique. Each participant described a problematic situation with respect to their sexual health and the resulting data were analysed using a grounded theory based method. Results indicate that young Quebec adults are faced with problematic situations with respect to physical aspects of their sexual life that can be triggered by three types of elements: a risky event, a subjective physical symptom or passively acquired information. These problematic situations generate three categories of information needs: the actual health state, potential consequences and cures. To answer these needs, a majority of participants turned to professional, personal and verbal sources. Situational, cognitive, and affective factors differentiated their information search process with different combinations of four information search activities: starting, chaining, browsing, and differentiating. Self-motivation and understanding of the problem represent the two main information uses. At a theoretical level, results indicates that the general information model (Choo 2006), the information use environment model (Taylor 1986, 1991) and the information search activities (Ellis 1989a, 1989b, 2005) can be used in the personal context of sexual health. At a practical level, this study enriches the knowledge of the criteria for selecting information sources in the context of sexual health.

Keyphrases: Québec (province), Information behaviour, Information need, Information search, Information source, Information use, Sexual health, Young adult, Quebec (province)
Youth engagement: the ethics of inclusion and exclusion

This study focuses on the engagement of children and youth in their communities and the ways  they are included in and excluded from community life. Using a content analysis of a small town  United States newspaper over a one-year period, examples of engagement were identified and  classified into 12 categories: programs, clubs and special events; fundraising and community service; business and community support; participation in community events; school events; athletic and other performances; employment; involvement in local planning and decision making;  serving as a community representative; visibility and recognition; criminal activity and accidents;  and use of public space. Examples of community exclusion were identified as well. Young people were engaged primarily through activities that were adult-directed or supervised, or organized through schools, churches, and youth clubs. There was little involvement in local planning,  decision making, or activism. Some evidence existed of peer teaching, leadership, and self-initiated activities, as well as intentional efforts by adults to give youth a greater voice in community  activities. Implications include several ethical issues regarding the role of young people in community life, particularly young children, and the need for greater awareness on the part of communities of the contributions young people can make.

Keyphrases: participation