NCube/papyrus huggingface dataset:
Développement et mise à l’essai de récits audio dans une activité de réflexion collective avec des infirmières accompagnant des jeunes mères ayant vécu de la maltraitance

INTRODUCTION: A significant proportion of young mothers in early childhood intervention programs have experienced childhood trauma. Nurses do not always feel supported, nor competent, to approach this topic with them. When used in a collective reflection activity, digital stories have been identified as relevant tools to support nurses in their practice. However, the development of these devices and their actual use remain poorly described. AIM: This report describes the development of digital stories and their utilization in a collective reflection activity with nurses in an early intervention program. METHOD: Two digital stories were created with reference to Fraiberg's (1975) work with young mothers who have experienced childhood trauma. A period of immersion with the team of nurses in their daily activities allowed a contextualized approach to recruit credible informants, conduct separate narrative interviews with a nurse and a young mother, and write two short stories. Five nurses participated in a collective reflection activity based on a reflective practice framework (Rolfe, 2011), the course of which was recorded iteratively to ensure ongoing support and co-supervision of the group facilitator. FINDINGS: Upon hearing the voice of a young mother and recognizing themselves in the short story of a nurse, participants shared repressed emotions, started to describe their own practice and engaged in a critique of the conditions that shape their professional practice. Initiating a dialogue on a sensitive and silenced issue, the conversation highlighted nurses’ emotional laboring. Reflective practice was essential to develop group facilitator skills that were supportive to initiate collective reflection and analysis. CONCLUSION: Theories are valuable tools to create digital story devices that have the power to initiate group mediated reflections on sensitive and unacknowledged issues. This project reveals that nurses in early childhood intervention programs could readily pursue further engagement in reflective practice with their peers on this generally unspoken topic; and that further attention is required to support emotional competence development so that nurses feel prepared to hear the voices of young women with a childhood history of abuse and neglect.

Keyphrases: digital stories, primary care nurses, collective reflection, childhood maltreatment, young mothers, emotional laboring, emotional competency, early intervention program
Perception des cliniciens, gestionnaires et usagers à l’égard des interventions de groupe offertes pendant la réadaptation en déficience physique

Introduction: The demand for physical rehabilitation services in auditory, language, motor or visual impairment is growing. Concerned about the access and the efficiency of the services, rehabilitation settings are called upon to increasingly offer group interventions. Objectives: The first objective of this study is to examine the facilitators and barriers to the implementation of group interventions in rehabilitation. The second objective is to explore the perception of clinicians, managers and patients of issues related to quality of care, costs and the achievement of goals during those interventions. Methodology: A multiple case study design was used. Clinicians and managers from five rehabilitation centres in the Montreal area were consulted using an online questionnaire (n = 162) and focus groups. Individual interviews were conducted with patients who participated in group interventions. Descriptive and quantitative statistics were performed for the results of the questionnaire. The qualitative data were grouped by themes and triangulated with the results of the questionnaire. Results: Key facilitators include patient’s interest, potential for influence among participants, and recognition of the value of these interventions. The main obstacles are schedule management, time required for reasonning and planning the interventions and having a critical mass of patients which is sometimes insufficient to form groups. Regarding the second objective, group interventions are perceived as safe. They should be offered for profit rather than for financial reasons and be linked to the achievement of patient goals. They must also be offered as a complement to individual interventions. Conclusion: Group interventions provide many benefits. A better understanding of the perceptions of key actors will facilitate their implementation as well as their sustainability. Recommendations are proposed to help managers and clinicians plan services and at thus maximize benefits for patients.

Keyphrases: Rehabilitation, Physical rehabilitation, Physical impairment, Group interventions, Facilitators and barriers, Quality of care, Accessibility, Costs, Safety, Effectiveness
Exploration de l’intégration des nouveaux savoirs infirmiers aux savoirs antérieurs chez des infirmières et infirmiers diplômés hors Québec, durant un programme d’intégration professionnelle

This Master’s thesis presents the results of an exploratory qualitative research, aimed at integrating recent nursing knowledge with previous knowledge among nurses having graduated outside Quebec (IDHQ), during a professional integration program. One problem is the poor result in meeting the requirements of the integration program. The purpose of this research is to explore in IDHQs in the process of learning, the integration of new knowledge with previous knowledge essential to the practice of nursing with adults and elderly hospitalized requiring nursing care of medicine and surgery. Mezirow's theory of transformational learning (1978) served as a frame of reference and guided the formulation of the purpose and research question. We were inspired by the criteria of the case method as proposed by Stake (1994), within a constructivist epistemological approach to develop our exploratory estimate. The target population of the study was all IDHQ enrolled in a college integration program in nursing in Quebec. The case was a group of IDHQ who were studying in such an integration program in a college environment in Montreal. The group consisted of 27 participants, recruited according to the suitability sampling method, from two cohorts of the program. The thematic analysis of the data collected through four focus groups was done according to Paillé and Mucchielli (2012) and resulted in a diagram (thematic tree). The diagram presents the four phases of knowledge integration by the IDHQ: openness to new knowledge; activation of prior knowledge where participants perceive a brake to integration; the overcoming of the barriers and challenges to the integration of knowledge, of which the change of status is a part of, and finally, these elements combine to arrive at the phase of putting into practice knowledge in the process of integrating new knowledge with prior knowledge, where the cognitive "battle" for the choice of knowledge prevails. We can think that the results of this research can be useful particularly in the improvement of the programs of professional integration, but also for the orientation of the NGOQ in units of care.

Keyphrases: Nursing knowledge, Graduate nurses outside Quebec, Integration program, Transformational learning of Mezirow,, Exploratory study
Culture du bloc opératoire, rétention infirmière et intention de quitter : une ethnographie focalisée

Problematic: Nurse retention in operating rooms is as much a problem in Quebec as it is worldwide. This problem persists even though organizational and individual factors are wellestablished, as much for the retention as for the intention to leave. Diminished surgical wait times and operating room access are preoccupations for the government, and training new nurses is costly and time consuming for the establishment. It is thus very important to understand nurse retention factors and the reasons motivating intention to leave within their context in daily life. The goal of this study is to investigate operating room culture, its effect on nurse retention, and the causes influencing nurses’ intent to leave, according to the perceptions of nurses working in a university hospital in an urban center. Methodology: A focused ethnography was undertaken in autumn 2017. Eleven semi-structured interviews (n=11) were conducted with nurses, along with six days of on-site observation. Informal conversations and field notes completed the data collection, after which a thematic analysis was conducted. Results: Five themes emerged: new nurse retention, usual turnover, the vicious cycle of shortage, the reality of shared responsibility, and factors favoring retention. Conclusion: This study confirms many factors from the Tourangeau et al. (2010) reference framework. This focused ethnography has shed light on the specific setting of operating rooms, and brought three new factors to its framework: disruptive behaviour, the improvement of reality-sharing, and better communication. Recommendations have been submitted based on these findings.

Keyphrases: Culture, Nursing, Culture, Operating room, Ethnography, Nurse’s retention, Nurse’s turnover, Nurse’s satisfaction, Nurse’s intent to leave, Doctor-nurse relation
Perceptions des conseillères en soins infirmiers et en pratique infirmière avancée quant à la qualité, à la pertinence et aux impacts d’une formation à l’Ennéagramme sur les compétences infirmières

During major transformations of the Quebec healthcare system, including following Bill 10, the nursing educators and advanced practice nurses face major challenges in ensuring quality care for the Quebec population (OIIQ, 2014), thus highlighting the importance of supporting their role and skills (OIIQ, 2014, 2015). Emotional intelligence is essential for the deployment of nursing skills (Cummings, Hayduk and Estrabrooks, 2005; Cummings et al., 2010; Horton-Deutsch and Sherwood, 2008; O'Connor, 2008; Smith, Profetto-McGrath and Cummings, 2009; Triola, 2007). While several models and training programs can enhance professional skills, through the development of emotional intelligence, the Enneagram model is emerging as a promising avenue (Bland, 2010; Lapid-Bogda, 2004, 2007). However, the use and impact of this model in a North American context is poorly documented. Based on Watson's humanistic-caring model (2012), this qualitative study aims to explore the perceptions of nursing educators and advanced practice nurses about the quality, relevance, and impact of an Enneagram training on the development and exercise of their professional skills. Analysis, following Miles and Huberman (2003)’s method, and interpretation of data collected from individual interviews with nine nursing educators and advanced practice nurses, shows that an Enneagram training can foster better self-awareness and understanding of others. As a result, this model could support the development of several nursing skills including: acting with humanism, collaboration, clinical leadership and supporting learning in clinical settings. In addition, the results allow for a number of recommendations to be made in order to improve the educational strategies of the training provided and the mobilization of knowledge gained through nursing practice. Finally, this research paves the way for a promising model in the development of a professional practice imbued with humanism in nursing. More research is needed to better understand the effects of the Enneagram on healthcare professionals, including nurses, in the development of a humanistic practice.

Keyphrases: Nurs, Advanced practice nurs, Competencie, Emotional intelligence, Enneagram, Mental health, Organizational change
The Role of Cardiotrophin-Like Cytokine Factor 1 on the Development of Atherosclerosis

Metabolic syndrome represents a major global health problem. Its rate is constantly increasing. Cardiovascular diseases emerging from dyslipidemia conditions are a worldwide leading cause of death and morbidity, despite extensive medical research and wide range of drugs targeting atherosclerosis, obesity, diabetes etc.  Recent findings suggest the existence and persistence of low-grade inflammation in pathogenesis atherosclerosis and obesity. The “symbiosis” between metabolic and immune system is substantial and any perturbation contribute to the development of altered metabolic conditions that ultimately culminate in such disorders as obesity and atherosclerosis. The overall goal of my Master internship was to characterise the effect of cardiotrophin-like cytokine factor 1 on development of atherosclerosis and validate a long half-life derivative of cardiotrophin-like cytokine factor 1 coupled with Fc fragment of immunolglobulin G. Cardiotrophin-like cytokine factor 1 is a cytokine of the immune system with immunoregulatory activity. Cardiotrophin-like cytokine factor 1 belongs to the interleukin 6 family of monomeric cytokines. Cardiotrophin-like cytokine factor 1 is efficiently secreted in the presence of cytokine receptor like factor 1, a soluble cytokine receptor. Cardiotrophin-like cytokine 1 possesses neurotrophic activities mediated through the receptor of ciliary neurotrophic factor. Cardiotrophin-like cytokine factor 1 is a high affinity ligand for sortilin. Genome-wide association studies indicated that plasma low-density lipoprotein cholesterol levels and cardiovascular disease are associated with single nucleotide polymorphisms variants regulating sortilin expression. It was observed that cardiotrophin-like cytokine factor 1 binds and activates transfectants co-expressing sortilin and receptor of leukemia inhibitory factor. Both receptors are expressed by myeloid cells and leukemia inhibitory factor is a potent inducer of anti-inflammatory M2 macrophage differentiation.  The overall objective was to investigate if the interaction of cardiotrophin-like cytokine factor 1 or cardiotrophin-like cytokine factor 1 coupled with Fc fragment of immunoglobulin G with atherosclerotic plaque macrophages will reduce the foam cell formation and development of plaque. Our work shows that cardiotrophin-like cytokine 1 expression in mice on LDLR-/- atherosclerosis model does not decrease the development of plaque. However, some results revealed a significant contribution of cardiotrophin-like cytokine factor 1 in gain of body mass without changes in food intake.

Keyphrases: Cardiotrophin-like cytokine factor 1, Inflammation, Macrophages, Immune System, Sortilin, Metabolic Syndrome, Atherosclerosis
Étude de l’apprentissage d’une tâche motrice : implication de la voie Akt-GSK-3

The production of the human's movements, and among many other animals, can be effortlessly executed during the realization of a complex motor task. These highly coordinated movements require the integration of several orders coming from different regions of the brain, notably the motor areas of the cerebral cortex, basal ganglia and the cerebellum. Despite the recent progress in neuroscience, motor learning mechanism is still not completely understood. The way that the brain integrates and memorizes the motor movements during a motor activity is still not completely known. The protein kinase B (PKB/Akt) is a serine/threonine kinase highly conserved among the mammals and control several cellular metabolisms such as the proliferation, the survival and the cellular plasticity. Akt has three isoforms (Akt1, Akt2 and Akt3) that are encoded by three distinct genes. It is important to note that Akt3 is more expressed in the brain than Akt1 and Akt2. Glycogen Synthase Kinase-3 (GSK-3) includes two isoforms, GSK-3alpha and GSK-3beta. Akt and the protein kinase GSK-3 are closely connected. Certainly, it is well established that Akt reduces the activity of GSK-3 alpha/beta by its phosphorylation on the serine 21/9 respectively. A disruption of the Akt/GSK-3 pathway has been linked to several pathologies such as depression, schizophrenia and the bipolar disorder. The mainstream of my research was to study the capacity of training, performance and memorization of a complex motor task among mice having a genetic Akt3 deletion (Akt3 KO). The results on the rotarod demonstrated that Akt3 deletion compromised rotarod performances in comparison to wild type mice (WT). Interestingly, my results demonstrated a change of the phosphorylation of GSK-3 alpha/beta and propose that the motor training deficit would be caused by GSK-3 alpha/beta hyperactivation, induced by the genetic deletion of Akt3. It is interesting to note that lithium chronic treatment, which is known to inhibit directly and indirectly the GSK-3 protein, corrected the training deficit of Akt3 KO mice. Our results have established an important implication of the Akt3/GSK -3 pathway in motor training.

Keyphrases: Akt3, GSK-3, Rotarod, Striatum, Lithium, Motor learning
Bioassay-guided antidiabetic potentials of Devil’s club (Oplopanax horridus) preparations from the traditional pharmacopeia of the Squamish and other first nations of British Columbia

An ethnobotanical approach has been used to identify medicinal plant species used by Canadian First Nations to treat Type 2 diabetes (T2D) symptoms. Devil's Club (Oplopanax horridus (Sm.) Miq.) was identified as one of these plants reported to possess anti-diabetic properties. The aim of this Masters thesis was to evaluate Devil’s club inner bark potential stimulation of glucose transport in C2C12 cells and inhibition of G6Pase enzyme in H4IIE hepatocytes using in vitro bioassays. Different preparations of devil’s club were used. Traditional preparations of medicinal plants are often made with hot water, so a hot water extract of the plant was used to mimic the traditional methods. Also, 80% ethanol extract of the plant was used to maximize the extraction of plant compounds. Other fractions prepared with solvents of increasing polarity (Hexanes, dichloromethane –DCM, ethyl acetate, methanol, water) and a pure compound (chlorogenic acid) were used to begin unraveling devil’s club active compounds. The results of this study showed that ethanol extract, DCM, and hexanes fractions significantly stimulated glucose transport in C2C12 cells after 18 h incubation with stimulation percentages of 204±4%, 201±14%, and 197±8% above DMSO vehicle control, respectively. Meanwhile, a statistical significant inhibition in glucose-6-phosphatase (G6Pase) activity (-24±4% compared with vehicle control) was observed when DCM fraction was tested. Hence, with respect to glucose homeostasis, the results confirmed that several preparations of devil’s club inner bark significantly stimulated muscle glucose transport and inhibited hepatic G6Pase activity. Further studies using the phytochemical fractionation approach are needed to isolate compounds and compare them together with their mixtures to enable better understanding of the synergistic and antagonistic effect and to understand the plant’s mechanisms of action and molecular targets.

Keyphrases: G6Pase, Type 2 diabetes, Glucose transport, Traditional medicine, Glucose homeostasis, Natural health products
L’effet de la suppression de la douleur sur la biomécanique tridimensionnelle du genou et de la cheville chez des patients gonarthrosiques

Objectives To verify whether or not pain suppression in patients who have knee osteoarthritis have an immediate effect on three-dimensional biomechanics of the knee and the ankle while walking on an instrumented treadmill.  Methods  Nine patients with painful patellofemoral osteoarthritis, with or without femoro-tibial osteoarthritis, who reported pain intensity ≥ 3/10 on a Numeric Rating Scale (NRS) before the test, were included in this study. Biomechanical assessment of the knee/ankle along with a regularly NRS evaluation were conducted before and 15 min after pain relief induced by an intra-articular injection of 5 mL lidocaine (1%). All participants were assessed with the KneeKGTM system.  Results All participants exhibited reduction in pain after lidocaine injection. They were grouped according to the magnitude of pain reduction: minimal (N = 1), much improved (N = 4), and substantial reduction (N = 4). Biomechanics changes in the knee or in the ankle were found in all subjects, but in a different plane from one subject to another. The participant who had minimal reduction in pain post injection demonstrated changes in sagittal and frontal planes at the knee, and three-dimensional changes at the ankle. In the much improved group at the knee, one subject demonstrated three-dimensional changes, another subject had changes in two planes (frontal and transverse) and only two subjects had changes in the frontal plane. At the ankle, all subjects had changes in at least two planes (frontal and transverse). One subject in the substantial reduction group demonstrated changes in two planes at the knee (frontal and transverse) and the other subjects had changes in only one plane (sagittal, frontal or transverse). In this group, all subjects had changes in at least two plans at the ankle.  Conclusion Reduction in pain has an effect 15 min after injection on three-dimensional kinematics of the knee and ankle during walking. All participants demonstrated kinematic changes at least in one anatomical plane at both joints.

Keyphrases: KneeKG, Pain, Knee osteoarthritis, Kinematic, Ankle
Role of zinc transporter LIV-1 protein in high-grade serous ovarian cancer

High-grade serous carcinoma (HGSC) of the ovary is the most lethal gynecological cancer. Although recent progress has advanced our understanding in the aggressiveness of HGSC, the molecular mechanisms involved in the development of this cancer remain unclear. One of the main features of aggressive tumours is their ability to metastasize. In general, epithelial ovarian cancers (EOCs) spread through local extension, lymphatic invasion, and intraperitoneal implantation. Studies have suggested that the zinc transporter LIV-1 protein has an important role in tumour metastasis in different tissues. However, there are no reports on the role of LIV-1 in HGSC metastasis. We investigated the role of LIV-1 in the invasive and migration potential of HGSC cells. We observed variable expression levels of the LIV-1 gene and protein in 14 HGSC cell lines. Although a positive correlation was observed between the gene expression and the quantities of LIV-1 protein, this correlation was not strong and not significant; thus, we chose five cell lines to perform biological assays, based on their protein levels: TOV3133G and TOV2295(R) (high), TOV2978G (medium), and TOV1369 and OV866(2) (low). We found that there was a strong and significant positive correlation between LIV-1 levels and the invasive potential of these HGSC cells (R2 = 0.8666, p = 0.0216), but a weak and negative correlation (not significant) with their migration potential (R2 = 0.4514, p = 0.2142). RNAi experiments demonstrated that reduced LIV-1 expression inhibits invasive capacity; however, no significant differences were observed in regard to migration capacity. Our results suggest that LIV-1 has a positive role in favouring the tumour progression of HGSC. We have demonstrated that LIV-1 is involved in the in vitro invasion but not migration of HGSC cells, and that its expression is associated with more aggressive ovarian cancers,suggesting LIV-1 as a potential marker for tumour aggressiveness.

Keyphrases: LIV-1, Cell invasion, Cell migration, Epithelial ovarian cancer, High-grade serous carcinoma (HGSC)