Miracle-dz/UniFAR-FTUs huggingface dataset:
Randomized Trial of a Supportive Psychotherapy for Parents of Adolescents and Young Adults With Hematologic Malignancies.

BACKGROUND Cancer regularly disrupts health and developmental trajectories in adolescents and young adults (AYAs). Parents have been shown to have a substantial impact on the health and cancer survivorship activities of AYA patients in the form of symptom management. However, no randomized controlled trial has evaluated a coping support intervention (CSI) program for parents of AYAs with cancer aged 18 to 40 years. PATIENTS AND METHODS From November 30, 2012, to August 29, 2016, parents of AYAs with hematologic malignancies were randomized in a phase III controlled trial (1:1 ratio, stratified sampling) to either the research-based CSI AYA-Parents group (CSI group; n=82) or the standard care (SC) group (n=70). CSI consisted of 5 sessions to achieve the enhancement of parental adaptive coping as the primary outcome (per the adaptive coping scale of the 28-item Brief COPE, a validated multidimensional self-assessment-questionnaire recommended for clinical cancer research). Measures of adaptive coping, depression, and mental health were collected at pre-CSI (measurement date T1), at the end of the intervention sessions (measurement date T2), and at follow-up (3 months). We calculated mean change scores in outcomes and estimated intervention effect sizes (Cohen's d) for changes from T1 to T2/T3, with 0.2 indicating a small effect, 0.5 a medium effect, and 0.8 a large effect. All statistical tests were 2-sided. RESULTS In the intention-to-treat analysis, the CSI group significantly improved their adaptive coping compared with the SC group (95% CI, 0.30-2.54; P=.013; d=0.405), whereas adaptive coping in the SC group deteriorated. The CSI group also experienced a significant decrease in depressive symptoms and improved mental health with clinical significance (95% CI, -1.98 to -0.30; P=.008; d=0.433, and 95% CI, -0.19 to 3.97; P=.074; d=0.292, respectively). Sensitivity analyses confirmed the robustness of the main intention-to-treat analysis. CONCLUSIONS CSI improved effectively adaptive coping and depression in parents of AYAs with hematologic malignancies. It may represent a novel family-based approach in AYA oncology care.
HIF‐1α plays an essential role in BMP9‐mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK1

Bone homeostasis is regulated by bone morphogenic proteins (BMPs), among which BMP9 is one of the most osteogenic. Here, we have found that BMP9 rapidly increases the protein expression of hypoxia‐inducible factor‐1α (HIF‐1α) in osteoblasts under normoxic conditions more efficiently than BMP2 or BMP4. A combination of BMP9 and hypoxia further increased HIF‐1α protein expression. HIF‐1α protein induction by BMP9 is not accompanied by messenger RNA (mRNA) increase and is inhibited by the activation of prolyl hydroxylase domain (PHD)‐containing protein, indicating that BMP9 induces HIF‐1α protein expression by inhibiting PHD‐mediated protein degradation. BMP9‐induced HIF‐1α protein increase was abrogated by inhibitors of phosphoinositide 3‐kinase (PI3K) and protein kinase B (AKT) kinase, indicating that it is mediated by PI3K–AKT signaling pathway. BMP9 increased mRNA expression of pyruvate dehydrogenase kinase 1 (PDK1), a glycolytic enzyme, and vascular endothelial growth factor‐A (VEGF‐A), an angiogenic factor, in osteoblasts. Notably, BMP9‐induced mRNA expression of PDK1, but not that of VEGF‐A, was significantly inhibited by small interference RNA‐mediated knockdown of Hif‐1α. BMP9‐induced matrix mineralization and osteogenic marker gene expressions were significantly inhibited by chemical inhibition and gene knockdown of either Hif‐1α or Pdk‐1, respectively. Since increased glycolysis is an essential feature of differentiated osteoblasts, our findings indicate that HIF‐1α expression is important in BMP9‐mediated osteoblast differentiation through the induction of PDK1.
Experiences and perceptions of final‐year nursing students of using a chatbot in a simulated emergency situation: A qualitative study

Abstract Aim The aim of this study is to explore the experiences and perceptions of final‐year nursing students on the acceptability and feasibility of using a chatbot for clinical decision‐making and patient safety. Background The effective and inclusive use of new technologies such as conversational agents or chatbots could support nurses in increasing evidence‐based care and decreasing low‐quality services. Methods A descriptive qualitative study was used through focus group interviews. The data analysis was conducted using a thematic analysis. Results This study included 114 participants. After our data analysis, two main themes emerged: (i) experiences in the use of a chatbot service for clinical decision‐making and and (ii) integrating conversational agents into the organizational safety culture. Conclusions The findings of our study provide preliminary support for the acceptability and feasibility of adopting SafeBot, a chatbot for clinical decision‐making and patient safety. Our results revealed substantial recommendations for refining navigation, layout and content, as well as useful insights to support its acceptance in real nursing practice. Implications for Nursing Management Leaders and managers may well see artificial intelligence‐based conversational agents like SafeBot as a potential solution in modern nursing practice for effective problem‐solving resolution, innovative staffing and nursing care delivery models at the bedside and criteria for measuring and ensure quality and patient safety.
Complete Genome Sequences of Lambdoid Phages 21, 434, and 434B and Several Lambda Hybrids

Recombinational hybrids between phage λ and its relatives were instrumental in the beginnings of molecular biology. Here, we report the complete genome sequences of lambdoid phages 21 and 434 and three of their λ hybrids. In addition, we describe 434B, where the entire lysis gene region was replaced by cryptic prophage sequences. ABSTRACT Recombinational hybrids between phage λ and its relatives were instrumental in the beginnings of molecular biology. Here, we report the complete genome sequences of lambdoid phages 21 and 434 and three of their λ hybrids. In addition, we describe 434B, where the entire lysis gene region was replaced by cryptic prophage sequences.
Classic mechanisms and experimental models for the anti‐inflammatory effect of traditional Chinese medicine

Inflammation is a common disease involved in the pathogenesis, complications, and sequelae of a large number of related diseases, and therefore considerable research has been directed toward developing anti‐inflammatory drugs for the prevention and treatment of these diseases. Traditional Chinese medicine (TCM) has been used to treat inflammatory and related diseases since ancient times. According to the review of abundant modern scientific researches, it is suggested that TCM exhibit anti‐inflammatory effects at different levels, and via multiple pathways with various targets, and recently a series of in vitro and in vivo anti‐inflammatory models have been developed for anti‐inflammation research in TCM. Currently, the reported classic mechanisms of TCM and experimental models of its anti‐inflammatory effects provide reference points and guidance for further research and development of TCM. Importantly, the research clearly confirms that TCM is now and will continue to be an effective form of treatment for many types of inflammation and inflammation‐related diseases.
Reproducibility and reporting, the routes to progress in breath research—highlights from the Breath Biopsy Conference 2021

With the continued presence of COVID-19 worldwide, it has been a challenge for the breath research community to progress with clinical studies and travel restrictions have also limited the opportunities to meet up, share ideas and celebrate the latest advances. The Breath Biopsy Conference 2021 offered the chance to catch up with the latest breath research and to share progress that researchers in the community have been able to make in these difficult times. Limited opportunities for clinical research have led many in the field to look more closely at different methods for breath collection and have contributed to the growing calls for consistent standards in how results are reported, shared and even how breath studies themselves are carried out. As such, standardization was a key theme for this year’s event and featured prominently in the keynotes, discussions and throughout many of the presentations. With over 900 registrants, almost 400 live attendees and 16 speakers, the Breath Biopsy Conference continues to bring together breath research leaders from around the world. This article provides an overview of the highlights from this event.
Pathophysiologic Mechanisms of Tobacco Smoke Producing Atherosclerosis

Introduction: Despite the convincing epidemiologic association between smoking and vascular disease, the pathophysiologic mechanisms by which smoking initiates and contributes to the progression of atherosclerosis remain incompletely understood. A precise dose-dependent correlation has never been demonstrated, suggesting that the biological relationship is complex and influenced by individual genetic and possibly environmental factors. Although endothelial dysfunction and intimal damage appear to be central to atherogenesis, how tobacco products cause this effect has not been established. The purpose of this review is to describe the current state of knowledge of the main pathophysiologic pathways of how tobacco smoking abets atherosclerosis Constituents of Tobacco Smoke: Tobacco combustion produces a mixture of organic substances derived from burning organic materials. The predominant gaseous phase constituents include carbon monoxide, acetaldehyde, formaldehyde, acrolein, and other carbonyls, as well as nicotine and tobacco-specific nitrosamines. Potential Pathophysiologic Mechanisms: Smoking-induced changes in coronary vasomotor tone, platelet activation, and endothelial integrity are major components of both the development of atherosclerosis and its clinical presentation. Smoking may initiate and accelerate the progression of atherosclerosis by injuring the vascular intima. Other potential mechanisms include intimal damage and endothelial dysfunction, oxidative stress and injury, thrombosis, lipid abnormalities, and inflammation. Conclusion: Smoking tobacco products contributes measurably to the incidence of acute vascular events and chronic disease. The causative compound, the exact mechanism of injury, and whether the atherogenic effect is modifiable are not known.
Immunotherapy in soft tissue sarcoma: current evidence and future perspectives in a variegated family of different tumor

ABSTRACT Introduction In the last few years, steps forward in the knowledge of the biology of soft tissue sarcomas (STS) have led to the development of new therapeutic strategies, including immunotherapy. Areas covered This review outlines the recent findings on immunological features and provides a synopsis of the results of clinical trials with different immunotherapy approaches in STS, discussing criticisms, and how the efficacy of immunotherapy could be improved. Expert opinion The heterogeneity of STS has limited generalized approaches of immunotherapy in the disease. Clinical decisions should encompass a comprehensive characterization of the tumor microenvironment (TME), marked by intra-histotype diversity. Profiling of immune cells, checkpoint molecules, and antigen target/HLA expression is deemed to reshape the classical histotype classification for a selection of the most appropriate immune-based treatment. In a synergistic view, tumor-directed treatments, designed on the genetic and epigenetic histotype make-up, should be monitored for their immunomodulant effect and applied to ensure or amplify immunotherapy response. In light of the dynamic nature of the TME, this immunomonitoring should be conducted at baseline and during treatment, for improved therapeutic decisions and rational sequence of treatment combination, pursuing an immunological marker approach by histotype guidance.
Hybrid Multimodal and Intermodal Transport Simulation: Case Study on Large-Scale Evacuation Planning

Transport simulation models exist on multiple scales, from the simulated evacuation of a nightclub with a few hundred guests to that of a transport hub such as a large train station to the simulated evacuation of a megalopolis in case of a tsunami. Depending on precision and complexity requirements, continuous (e.g., force-based, velocity obstacle–based), spatiotemporal discrete (e.g., cellular automata), or queue models are applied. In general, the finer the spatiotemporal resolution, the more precise are the interactions captured between travelers (e.g., pedestrians or vehicles), but the computational burden increases. The obvious approach to achieve higher computational speeds is to reduce the physical complexity (e.g., by using a queue model), which in turn reduces the precision. One way to increase the computational speed while retaining sufficient precision to make a reliable prognosis is to combine models of different scale in a hybrid manner, in which a finer model is applied where needed and a coarser model where plausible. This paper discusses an application of a hybrid simulation approach in the context of a large-scale multimodal and intermodal evacuation scenario. The presented case study investigates the feasibility of an evacuation of parts of the city of Hamburg, Germany, in case of a storm surge.
Face‐Processing Differences Present in Grapheme‐Color Synesthetes

Abstract Grapheme‐color synesthesia is a heterogeneous neurological phenomenon whereby the experience of a grapheme automatically and involuntarily elicits an experience of color. While the majority of synesthesia research has focused on inducer‐specific influences of synesthetic associations, more recent efforts have examined potential broader differences. Based on spontaneous reports from synesthetes detailing problems with face recognition, in conjunction with the geographical proximity of neurological regions relevant to both synesthesia and face processing, we sought to examine whether synesthetes demonstrated atypical face‐processing abilities. A total of 16 grapheme‐color synesthetes and 16 age‐and‐gender matched controls (±3 years) completed the Cambridge Face Memory Test (CFMT; Duchaine & Nakayama, 2006) of face memory, the Vanderbilt Holistic Face Processing Task (VHPT‐F; Richler, Floyd, & Gauthier, 2014) of holistic face processing, as well as a standardized self‐report questionnaire the Faces and Emotions Questionnaire (Freeman, Palermo, & Brock, 2015). The results revealed significantly poorer performance in synesthete's ability to recognize faces in the CFMT that was driven by a reduction in upright advantage. Results also revealed a significant reduction in overall accuracy on the VHPT‐F for synesthetes, who despite this displayed a comparable holistic processing advantage compared to matched controls. Finally, synesthetes also rated themselves as significantly worse at face recognition. We suggest that this pattern may reflect differences in the development of individualized perceptual strategies.
Numerical Simulation and Justification of Antagonists in Isometric Squatting

Introduction Lombard’s paradox [1][2], the observation that bi-articular muscles can be observed active in opposition to one of their joint moments, is one of several phenomena related to the control of human muscles that are difficult to understand from an intuitive point-of-view. Another example is mono-articular antagonists. When interpreting the effects of particular muscle actions is important to bear in mind, that the musculo-skeletal system is a system of interconnected elements and must be studied as such. In-vivo experiments to obtain detailed information about the control of individual muscles are technically and/or ethically difficult to conduct, and the basis of the present work is consequently the idea that much insight can be obtained from the study of numerical models, provided they can be shown to reproduce the experimentally observed phenomena. This paper describes some simple simulations that are the basis for the development of a general body modeling software system by the name of AnyBody. We show that a simple mechanical model of the human leg can reproduce Lombard’s paradox, predict mono-articular antagonists, and provide rational explanations for both phenomena.
Transfer of continuous manufacturing process principles for mAb production in a GMP environment: A step in the transition from batch to continuous

Implementation of continuous in lieu of batch upstream processing (USP) and downstream process (DSP) for the production of recombinant therapeutic protein is a significant paradigm change. The present report describes how the first kilograms of monoclonal antibody were produced with equipment originally designed for batch operations while using continuous manufacturing processes and principles. Project timelines for the delivery of clinical material have driven this ambition and helped the transition. Nevertheless, because of equipment availability, a tradeoff between the envisaged continuous downstream process (cDSP) operations and the ones described in this article had to be taken. A total of 2.1 kg of monoclonal antibody were produced in two GMP runs for clinical trials. For USP, a 200‐L single‐use pilot scale bioreactor was upgraded to enable perfusion operation. DSP steps were designed to be easily transferable to cDSP for later clinical or commercial productions. An in‐line conditioning buffer preparation strategy was tested in a discontinuous way to prove its efficiency and the purification cascade was structured in parallel to the continuous collection of antibody‐containing cell culture supernatant. This strategy will avoid any process change when later moving to the continuous equipment that is currently under qualification. Alignment between small‐scale references runs and the GMP runs in terms of productivity and quality confirmed that the presented approach was valid. Thus, we demonstrate that existing fed‐batch infrastructure can be adapted to continuous manufacturing without significant additional investments. Such approach is useful to evaluate next‐generation manufacturing processes before making large investments.
Application of 3-Dimensionally Printed Coplanar Template Improves Diagnostic Yield of CT-Guided Percutaneous Core Needle Biopsy for Pulmonary Nodules

Background and Objective: Computed tomography-guided percutaneous lung biopsy is a commonly used method for clarifying the nature of nodules, masses or lung consolidation. However, the diagnostic yield of nodules needs to be improved when compared with masses during percutaneous lung biopsy. In recent years, 3D-printed coplanar templates have been gradually utilized in radioactive seed implantation for lung cancer treatment. However, there is little research on the application of 3D-printed coplanar templates in pulmonary nodules biopsy. Therefore, we conducted a single center and retrospective study to explore the application value of 3D-printed coplanar puncture template-assisted computed tomography-guided percutaneous core needle biopsy of small pulmonary nodules. Methods: 210 patients hospitalized in Taihe Hospital with pulmonary nodules underwent percutaneous core needle biopsy for histopathology diagnosis and were included in the study. 106 patients underwent conventional percutaneous lung biopsy (control group) and 104 patients underwent 3D-PCT-assisted percutaneous lung biopsy (3D-PCT group). The diagnostic yield and incidence of complications were recorded and compared between the two groups. Results: The overall diagnostic yield significantly improved in 3D-PCT group (95.2%) compared with Control group (87.7%) (P < .05); the diagnostic yield for lung nodules smaller than 2 cm in the 3D-PCT group and the control group was 94.4% and 80.5%, respectively, (P < .05). Incidence of pneumothorax (17.3% vs 18.9%) and pulmonary hemorrhage (7.7% vs 9.4%) were not significantly difference between the two groups (P > .05). Conclusions: Studies indicated that application of 3-Dimensionally printed coplanar template improves diagnostic yield of CT-guided percutaneous core needle biopsy for pulmonary nodules, especially for pulmonary nodule smaller than 2 cm.
Targeting CD73 with AB680 (Quemliclustat), a Novel and Potent Small-Molecule CD73 Inhibitor, Restores Immune Functionality and Facilitates Antitumor Immunity

Abstract T cells play a critical role in the control of cancer. The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. However, durable clinical benefit is observed in only a subset of patients, prompting research efforts to focus on strategies that target multiple inhibitory signals within the tumor microenvironment (TME) to limit tumor evasion and improve patient outcomes. Adenosine has emerged as a potent immune suppressant within the TME, and CD73 is the major enzyme responsible for its extracellular production. CD73 can be co-opted within the TME to impair T-cell–mediated antitumor immunity and promote tumor growth. To target this pathway and block the formation of adenosine, we designed a novel, selective, and potent class of small-molecule inhibitors of CD73, including AB680 (quemliclustat), which is currently being tested in patients with cancer. AB680 effectively restored T-cell proliferation, cytokine secretion, and cytotoxicity that were dampened by the formation of immunosuppressive adenosine by CD73. Furthermore, in an allogeneic mixed lymphocyte reaction where CD73-derived adenosine had a dominant suppressive effect in the presence of PD-1 blockade, AB680 restored T-cell activation and function. Finally, in a preclinical mouse model of melanoma, AB680 inhibited CD73 in the TME and increased the antitumor activity of PD-1 blockade. Collectively, these data provide a rationale for the inhibition of CD73 with AB680 in combination with ICB, such as anti–PD-1, to improve cancer patient outcomes.
Applied research on band notched techniques in planar printed monopole antennas

The Coplanar Waveguide(CPW) fed circular monopole antenna is improved to suppress the interference among different wireless systems.An ultra wideband(UWB) antenna with dual band notched characteristics and a tri-band antenna for WLAN/WiMAX applications are,respectively,proposed in this paper.The UWB antenna with the measured band rejection of 3.29～4GHz and 5.29～6GHz is designed by loading two pieces of polygonal slotline at the bottom of the circular patch.Its notched frequency bands cover WLAN and WiMAX systems.In the design of the tri-band antenna,the frequency band of the antenna is controlled and notched by loading the elements of slotline,resonant stub and radial slot on the antenna.This tri-band antenna renders the measured frequency bands of 2.25～2.8GHz,3.3～3.8GHz and 5.1～5.9GHz,which cover the application bands of WLAN/WiMAX,and has good band rejected characteristics in the remaining frequency band.Both the proposed antennas are of adjustable frequency band,and are suitable for suppressing the interference among wireless systems in the complex circumstance.