Miracle-dz/UniFAR-FTUs huggingface dataset:
Serogroup Y Clonal Complex 23 Meningococcus in China Acquiring Penicillin Resistance from Commensal Neisseria lactamica Species

Invasive meningococcal disease (IMD) due to serogroup Y Neisseria meningitidis (NmY) is rare in China; recently, an invasive NmY isolate, Nm512, was discovered in Shanghai with decreased susceptibility to penicillin (PenNS). Here, we investigated the epidemiology of NmY isolates in Shanghai and explored the potential commensal Neisseria lactamica donor of the PenNS NmY isolate. ABSTRACT Invasive meningococcal disease (IMD) due to serogroup Y Neisseria meningitidis (NmY) is rare in China; recently, an invasive NmY isolate, Nm512, was discovered in Shanghai with decreased susceptibility to penicillin (PenNS). Here, we investigated the epidemiology of NmY isolates in Shanghai and explored the potential commensal Neisseria lactamica donor of the PenNS NmY isolate. A total of 491 N. meningitidis and 724 commensal Neisseria spp. isolates were collected. Eleven NmY isolates were discovered from IMD (n = 1) and carriers (n = 10), including two PenNS isolates with five-key-mutation-harboring (F504L-A510V-I515V-H541N-I566V) penA genes. Five of the eight ST-175 complex (CC175) isolates had a genotype [Y:P1.5-1,2-2:F5-8:ST-175(CC175)] identical to that of the predominant invasive clone found in South Africa. Only one invasive NmY CC23 isolate (Nm512) was discovered; this isolate carried a novel PenNS penA832 allele, which was identified in commensal N. lactamica isolates locally. Recombination analysis and transformation of the penA allele highlighted that N. meningitidis Nm512 may acquire resistance from its commensal donor; this was supported by the similar distribution of transformation-required DNA uptake sequence variants and the highly cognate receptor ComP between N. meningitidis and N. lactamica. In 2,309 NmY CC23 genomes from the PubMLST database, isolates with key-mutation-harboring penA genes comprised 12% and have been increasing since the 1990s, accompanied by recruitment of the blaROB-1 and/or quinolone resistance allele. Moreover, penA22 was predominant among genomes without key mutations in penA. These results strongly suggest that Nm512 is a descendant of the penA22-harboring CC23 isolate from Europe and acquired its penicillin resistance locally from commensal N. lactamica species by natural transformation.
Antecedents and consequences of open and closed questions in intake sessions of psychodynamic psychotherapy

ABSTRACT Objective: To investigate whether there are different antecedents and consequences of different types of therapist questions as this has implications for conducting psychotherapy and for training therapists. Method: We examined the antecedents and consequences of questions for 88 clients working with 33 doctoral student therapists in psychodynamic psychotherapy. Questions were coded into open questions for thoughts (OQT), open questions for feelings (OQF), closed questions for facts (CQF), and closed questions other (CQO). The antecedents and consequences were assessed in terms of self-referring pronouns (SRP), self-referring emotion words (SRE), and number of words. Results: In terms of antecedents, when clients were using a high number of SRP, therapists were more likely to ask OQT and CQO than CQF. When clients were using a high number of SRE, therapists were more likely to ask OQF than CQF. In terms of consequences, clients spoke less after CQF than the other three skills, used fewer SRP after CQF than after CQO, and used more SRE after OQF than CQF. Conclusions: CQO were more similar in terms of antecedents and consequences to OQT and OQF than to CQF.
The Impact of Mother-Infant Facial Synchrony

Various measures of infant responsiveness have been shown to predict child outcomes. Despite this extensive research, there is no work examining links between infant responsiveness during caregiver-infant interactions with infants' ability to perform basic linguistic tasks. One key task in early linguistic development is word segmentation, an achievement that allows infants to build their mental dictionaries. We hypothesized that infants' responsiveness to caregiver facial expressions might be related to their word segmentation ability. In order to test this hypothesis, mothers came into the lab and were videotaped reading books containing target words to their 5-month-old children. After the infants were read to, we tested their listening preference for words in the books, as well as novel words; this test yielded a preference score (preference for familiar vs. unfamiliar words). We also used the videotaped reading to code facial expressions for both infant and caregiver, and subsequently, we tabulated occasions where synchronous facial expressions occurred for each member of the dyad. We then examined possible correlations between our preference score and measures gleaned from the dyadic facial expression coding. Although neither the number of infant-led synchronous facial expressions nor the total number of facial expressions produced by either member was significantly correlated with preference score, our measure of synchronous facial expressions led by the caregiver was highly correlated with preference score. Thus, results support the hypothesis that infant responsiveness during caregiver-infant interaction, as indexed by synchronous facial expressions with caregivers, may be related to language learning ability.
Detection and Molecular Characterization of Two Potyvirus Species on Cucurbits from Northwestern of Iran

Article history: Received 29 August 2020 Accepted 02 November 2020 Available online 17 November 2020 Cucurbit crops belonging to the family Cucurbitaceae are one the most important horticultural crops worldwide and cultivated widely in Iran. To aim detection and phylogenetic analysis of potyvirus members, during 2015-2016, 215 leaf samples from cucumber, melon, watermelon, and squash symptomatic with deforming and reduction in leaf size, blistering, mild and severe mosaic, fruit deformation, and stunting were collected from 10 major cucurbitsgrowing locations in West Azerbaijan province. Following mechanical inoculation of Cucurbita pepo as an indicator plant with symptomatic cucurbit crop extracts, a variety of symptoms developed on the plants. Then, total RNA extracted from 70 symptomatic leaf samples, and partial RNA-dependent RNA polymerase (NIb) (350 bp) was amplified using universal primer pairs (NIb2F/NIb3R) by reverse transcription-polymerase chain reaction (RT-PCR). The infection by potyviruses in 38.75% of the samples was confirmed based on the result. The five PCR products belonging to different hosts at the expected size (350bp) were sequenced. One sample (Ir-Na: MH491979) was determined to be infected by the Watermelon mosaic virus (WMV) whereas four samples (Ir-Ma:MH491979, Ir-Ng:MH491980, Ir-Pi: MH491981, and IrUr: MH491982) infected by Zucchini yellow mosaic virus (ZYMV). In the phylogenetic analysis based on Maximum-likelihood, three ZYMV isolates (Ir-Ng, Ir-Pi, and Ir-Ur) were placed in subgroup I whereas one isolate (Ir-Ma) was placed in subgroup II belonging to group A. Also, WMV isolate (IrNa) was placed in CL phylogroup.  2021UMZ. All rights reserved.
EXTENSIONS OF EULER-TYPE SUMS AND RAMANUJAN-TYPE SUMS

We define a new kind of classical digamma function, and establish some of its fundamental identities. Then we apply the formulas obtained, and extend tools developed by Flajolet and Salvy to study more general Euler-type sums. The main results of Flajolet and Salvy’s paper (Expo. Math. 7(1) (1998), 15–35) are immediate corollaries of the main results in this paper. Furthermore, we provide some parameterized extensions of Ramanujan-type identities that involve hyperbolic series. Some interesting new consequences and illustrative examples are considered.
Title-can be set in "File -> Properties"

Depleted Monolithic Active Pixel Sensors (DMAPS), also known as depleted CMOS sensors, are extremely attractive for particle physics experiments. As the sensing diode and readout electronics can be integrated on the same silicon substrate, DMAPS remove the need for hybridization. This results in thin detectors with reduced production time and costs. To achieve high speed and high radiation tolerance, DMAPS are manufactured in High Voltage (HV) processes on High Resistivity (HR) wafers. Today’s most performant DMAPS are 50 μm thin and have 50 μm x 50 μm cell size with integrated mixed analog and digital readout electronics, 11 ns time resolution and 5 x 10 1 MeV neq/cm radiation tolerance. DMAPS in HR/HV-CMOS have been adopted as the sensor technology for the pixel tracker for the Mu3e experiment and are under consideration for the ATLAS detector Phase-II Upgrade. However, in spite of the major improvements demonstrated by DMAPS, further research to achieve even more performant sensors is needed to realize the full potential of these sensors to meet the most challenging requirements for particle physics experiments planned for the future. This article describes the state-of-the-art of DMAPS in terms of time resolution and radiation tolerance, and presents specific work done by the CERNRD50 collaboration to further develop the performance of these sensors.
Health Risk Assessment Levels of Selected Heavy Metals on Solanum Nigrum, Soils and Water Collected Along River Moiben, Uasin-Gishu County, Kenya

The study investigated heavy metals’ levels in soil, Solanum nigrum and irrigation water from intense Moiben irrigated farms located in Meibeki Karuna, Uasin Gishu County. Purposive sampling was used to identify farms where there is an intense irrigated agricultural practice. Irrigation water, soil, and Solanum nigrum were randomly sampled, digested, and analyzed for heavy metals with the use of ICP-OES. SPSS Version 21 was used to analyse data statistically and means, Pearson Correlation coefficient, One-Way ANOVA and transfer factor were calculated. The heavy metals were compared from different sites. The soil mean concentration for Zn, Fe, Pb, Mg, Cr, Cu and Cd ranged between 46.90-0.18, 352–181, 10.75-9.91, 1.52-1.34, 10.14 9.67, 12.85 12.03 and 0.72 0.53 mg/kg correspondingly. Irrigation mean water concentration for Zn, Fe, Pb, Mg, Cr, Cu and Cd ranged between 0.071-0.054, 0.810. 669, 0.05-0.00, 0.05 – 0.045, 0.055-0.015, 0.27-0.197 and 0.005-0.001 mg/l respectively. Solanum nigrum mean concentration for Zn, Fe, Pb, Mg, Cr, Cu and Cd ranged between 0.001209 0.000592, 0.004003 0.002627, 0.000329 0.000079, 0.000837– 0.000565, 0.000104-0.000089, 0.000292-0.000204 and 0.000329-0.000004 mg/kg, respectively. One-way ANOVA indicated spatial significantly different in soil, water, and Solanum nigrum. The metal levels in soil were below WHO/FAO standards except Fe and Pb in irrigation water. Levels of Fe as well as Pb in Solanum nigrum were within WHO/FAO standards. Solanum nigrum are safe for consumption nevertheless continuous surveillance of heavy metals in soil, vegetables and irrigation water is vital preventive strategy to avoid accrual of heavy metals in the food chain.
IN SILICO APPROACH OF COLLAGEN FROM TUNA FISH BY-PRODUCT AS ANGIOTENSIN-CONVERTING ENZYME INHIBITOR

Objective: This study explores the sustainable valorization of by-products from tuna fish based on in silico approach. Methods: In silico approaches (BIOPEP database, PeptideRanker database, peptide calculator [PepCalc] database, and toxin prediction [ToxinPred] database) were employed to evaluate the potential of collagens from tuna as a potential source of bioactive peptides. Furthermore, primary structure, biological potential, physicochemical, sensory, and toxicity characteristics of the theoretically released angiotensin-converting enzyme (ACE) inhibitor collagen peptides were predicted. Results: Tuna collagen was selected as a potential precursor of bioactive peptides based on in silico analysis. Most notable among these are ACE inhibitory peptides. First, the potential of tuna collagen for the releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are many bioactive peptides in tuna collagen sequences. Then, an in silico proteolysis using selected enzymes (papain and pepsin) to obtained ACE inhibitory peptides was investigated and then analyzed using PeptideRanker and PepCalc. Cytotoxicity analysis using the online toxic prediction tool ToxinPred revealed that all in silico proteolysis-derived ACE inhibitory peptides are non-cytotoxic. Conclusions: Overall, the present study highlights that the tuna collagens could be a promising precursor of bioactive peptides that have an antihypertensive effect (ACE inhibitory activities) for developing functional food or nutraceutical products.
Community, network or both? Towards a holistic approach to studying online social structures

This paper overviews scientific narratives surrounding communities and networks both off- and online and criticizes the dichotomous approach to the topic, according to which each social structure can be classified as either a community or a network. It is argued that such a division does not facilitate comprehension of the contemporary online social structures with their complexity and dynamism. The study provides an alternative view on the issue assuming that community and network are not mutually exclusive concepts and can be studied holistically. The proposed theoretical statement is operationalized and piloted on the example of ‘Aarhus Internationals’ Facebook group – an online venue for international expats in Denmark. A content analysis of the group`s posts showed how exactly community and network aspects of social structures may coexist and interact online.
APPLICATION NOTE Ultra Performance Liquid Chromatography (UPLC) Further Improves Hydrogen/Deuterium Exchange Mass Spectrometry

Ultra performance liquid chromatography (UPLC) employs particles smaller than 2 m in diameter to achieve superior resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). We have tested the suitability of UPLC for the analysis of deuterated peptides in hydrogen exchange mass spectrometry experiments. Superior resolution and sample throughput were obtained with UPLC versus HPLC. For highly deuterated model peptides, deuterium loss using UPLC was greater than the deuterium loss observed using a conventional HPLC system, primarily as a result of the injection requirements of the UPLC system. Partially deuterated cytochrome c peptides also lost more deuterium in UPLC versus HPLC, although the effect was not as pronounced as it was for the highly deuterated model peptides. The exceptional chromatographic aspects of UPLC make it a very attractive alternative to HPLC for hydrogen exchange mass spectrometry experiments. (J Am Soc Mass Spectrom 2006, 17, 163–167) © 2006 American Society for Mass Spectrometry
Design and Analysis of a K-band Wideband VCO with Robust Start-up and Frequency Boost

This paper presents a K-band differential voltage-controlled oscillator (VCO) with robust start-up. A hybrid technique that combines open-loop digitally-controlled tail current with switchable auxiliary cross-coupled pairs (ACCPs) is proposed to ensure robust start-up for wideband operation. Compared with prior close-loop current controlling method, the proposed technique does not suffer from phase noise degradation. Furthermore, by evenly distributing the ACCPs, transistors with smaller size are allowed to achieve the same start-up condition, which leads to frequency boost and phase noise improvement. Implemented in 0. 18μm CMOS technology, the VCO achieves a wide frequency tuning range of 21.4 26.29 GHz (20.5%) and a low phase noise of -108.8 dBc/Hz at 1 MHz offset from 25.8 GHz carrier frequency. At 1.1 V power supply, the power consumption of the core circuit is 7.9 11.4 mW across the entire output frequency range.
Engineering And Science Institute: An Innovative Partnership To Provide Seamless Undergraduate Education

: WSU Vancouver was established in 1989 and was mandated by the Legislature to provide upper division and graduate education. Undergraduate students transfer to WSU Vancouver following a 2+2 model where the lower division coursework is often completed at a local community college. An extensive survey of high school students in the service region of the university indicated that the engineering and science students are least likely to utilize the 2+2 transfer option. In response to these findings, the WSU Vancouver Engineering and Science Institute was conceived as a way to provide a seamless four-year experience. In this unique model, faculty from two partnering community colleges moved to the WSU Vancouver campus to provide the lower division curricula in Mechanical Engineering, Computer Science and Biology. The lower division courses were re-designed to create seamless four-year programs offered by the three institutions on the university campus. This paper describes the Institute and highlights its unique aspects as a model that can be adapted in other states. The detailed development of the ME curriculum is used as the example of how the partnership process worked over the past two years to create a unique program.
The 7 International Seminar on Tropical Animal Production Contribution of Livestock Production on Food Sovereignty in Tropical Countries September 12-14, Yogyakarta, Indonesia

Angus Grade Black Cattle is one result of the artificial insemination (AI) program that is able to grow and adapt to tropical conditions, particularly in Sragen district. The aim of this study was to identify the relationship among body measurements in Angus Grade Black Cattle. We analyzed the phenotype of Angus Grade Black Cattle which as a result of a crossing between possibly consists of some Indonesian local cattle and Abeerden Angus Cattle. A total of 100 cows were used. The parameters recorded were: body length (BL), withers height (WH), rump height (RH), rump length (RL), rump width (RW), heart girth (HG), shoulder width (SW), chest depth (CD), hips width (HW), face length (FL), face width (FW), ear length (EL), ear width (EW) and tail length (TL). The results showed that the mean body weight (BW) was 407,742 kg while the body measurements were 129,213 cm (BL), 125,131 cm (WH), 133,940 cm (RH), 34,235 cm (RL), 13,854 cm (RW), 170,287 cm (HG), 41,228 cm (SW), 68,382 cm (CD), 47,377 cm (HW), 46,624 cm (FL), 21,676 cm (FW), 24,473 cm (EL), 15,744 cm (EW), 120,207 (TL) respectively. The highest correlation was obtained between HG-BW while the correlation between WH-BW was observed to be the least. There were six models of equation for predicting live body weight, the components were extracted explaining for the total variation of each model were 88,6 %, 1,7 %, 0,9 %, 0,8 %, 0,7 %, 0,6 % respectively of the variability in live body weight when all the body measurements were used in the equation. The body measurements could be used as selection criteria for improving body weight of Angus Grade Black Cattle. Angus Grade Black Cattle can be used for the breeding program as beef cattle.
Az első magyarországi humán papillomavírus központ tapasztalatai ( 2007 – 2011 )

A humán papillomavírus (HPV) a XXI. század leggyakoribb nemi úton terjedő kórokozója. A daganatkeltő (onkogén) HPV-típusokkal történő fertőzés tehető felelőssé a méhnyakrákok 99,7%-ának kialakulásáért. A megelőzésben döntő szerepe van a szűrésnek, amely a méhnyakrák előfordulását és halálozását bizonyítottan csökkentheti. Jelenleg 16 női HPV-centrum működik országszerte, amelyekben egységes irányelvek alapján végeznek szűréseket, tanácsadást és gondozást. Az első központot 2007 márciusában jelen közlemény szerzői hozták létre Budapesten, a Semmelweis Egyetem II. Számú Szülészeti és Nőgyógyászati Klinikáján. Betegek és módszerek: A szerzők 1155 páciensnél végeztek HPV-tipizálást, a vírus DNS-ének PCR-módszerrel való kimutatásával. Vizsgálták, hogy ebben a nem válogatott népességben milyen arányban mutatható ki a HPV-vel való fertőzöttség, milyen a különböző genotípusok, illetve a magas kockázatú (onkogén) típusok aránya, milyenek a fenti mutatók korcsoportokra vetítve, valamint milyen arányban mutatható ki egyidejűleg citológiai eltérés. A szerzők a központban elvégzett szűrések adatait összehasonlították a fejlett országokban talált eredményekkel, és választ kerestek arra, vajon magasabb-e a vírussal való fertőzöttség aránya a hazai népességben. Eredmények: A páciensek 55,5%-ában volt kimutatható a vírus valamelyik típusa, 38,5%-ában az onkogén típusok egyike. A HPV-vel való fertőzöttség aránya a 15 és 25 év közötti korosztályban volt a legmagasabb (62,9%). A magas kockázatú típusok között a 16-os volt a leggyakoribb, 19,5%-os aránnyal. A magas kockázatú vírussal való fertőzöttség mellett 32,1%-ban fordult elő egyidejű citológiai eltérés, s ezek több mint kétharmadában (70,6%) több magas kockázatú típus egyidejű jelenlétét igazolták. A citológiai eltéréssel járó esetekben 32%-ban volt kimutatható a 16-os típus. Következtetések: A szerzők által vizsgált, nem válogatott népesség fertőzöttségének aránya meghaladja a nemzetközi adatokat. Figyelemre méltó a legfi atalabb korosztály magas fertőzöttségi aránya, valamint a több onkogén típussal való egyidejű fertőzöttséggel járó magasabb kockázat. A 16-os és a 18-as típus magas daganatkeltő képessége szembetűnő az általunk vizsgált csoportban, de nem elhanyagolható az 51-es és a 31-es típus szerepe sem. Orv. Hetil. , 2011, 152, 1804–1807.
Deletion of Ip36 as a Primary Chromosomal Aberration in Intestinal Tlimorigenesis1

Cytogenetic analysis of short-term cultures from benign intestinal tu mors revealed clonal chromosome aberrations in five colorectal adenomas, one adenoma of the papilla Vateri, and one hyperplastic polyp of the rectum. One adenoma had numerical aberrations only, but in all other tumors structural rearrangements were found that led to loss of genetic material from Ip. In three of the cases, the deletion was restricted to the Ip36 band; the rest had lost larger Ip segments. The rearrangement of chromosome 1 was the sole karyotypic anomaly in three adenomas, all with mild or moderate dysplasia, and in the hyperplastic polyp. Both adenomas that had additional aberrations beyond the Ip loss showed severe dysplasia. We conclude that cytogenetically detectable loss of ge netic information from Ip36 is an early, seemingly primary, premalignant event in intestinal tumorigenesis. The fact that the adenomas with Ipas the sole change showed only mild or moderate dysplasia and that the del(lp) was found also in the hyperplastic polyp suggests that this aber ration is more related to the induction of hyperproliferation than to dif ferentiation disturbances in the intestinal mucosa.
RESEARCH ON LATERAL STRESS-STRAIN RELATION ON SIDE OF FOUNDATION PIT WITH LATERAL UNLOADING

Taking soil on the side of foundation pit excavation with k 0consolidation-lateral unloading plane strain experiment on plane strain apparatus,the testing results indicate that the shear strength indices of soil are obviously different between the lateral unloading and routine triaxial tests.Experimental analyses show that curves of lateral stress-strain under lateral unloading are also similar to a hyperbolic type.The lateral stress-strain relation of actual stress path and lateral unloading soil on the side foundation pit is deduced using Duncan-Chang model.Results between experimental data and model predicated are coincided very well.