Miracle-dz/UniFAR-FTUs huggingface dataset:
EFFECT OF USING LACTOBACILLUS ACIDOPHILUS ON E.COLI CAUSING EMBRYONIC DEATH AND LOW HATCHABILITY IN BALADY HATCHERIES AT DAKAHLIA GOVERNORATE

A total 550 swabs were collected from balady hatcheries egg shell surface (100), infertile eggs (100), dead in shell embryos (200) and newly hatched chicks (150) for bacteriological examination. The following bacteria were isolated and identified : E.coli, Proteus spp. , Pseudomonas aeruginosa, Citrobacter spp. , Enterobacter spp. , Staphylococcus spp. and Streptococcus spp. The most predominant isolated bacteria was E.coli (25.45%). Experimental infection for eggs were done by dipping in broth culture containing 1 × 10 6 CFU/ml of E.coli and in 3 × 10 8 CFU of Lactobacillus acidophilus for studying the effect of it in controlling E.coli infection and improvement of hatchability rate. The criteria used for judgment of therapeutic effect were mortality rate and rate of reisolation of E.coli from dead in shell embryos and newly hatched chicks. Dipping eggs in broth culture of Lactobacillus acidophilus then after 6 hours in broth culture of E.coli gave best results with low mortality rates and rate of reisolation than using them concurrently.
A protective role for Drosophila Filamin in nephrocytes via Yorkie mediated hypertrophy

Nephrocytes lacking key components of the filtration barrier respond by increasing Drosophila Filamin expression, which accumulates at the cell cortex to elicit a protective role. Podocytes are specialized epithelial cells of the kidney glomerulus and are an essential part of the filtration barrier. Because of their position, they are exposed to constant biomechanical forces such as shear stress and hydrostatic pressure. These forces increase during disease, resulting in podocyte injury. It is likely podocytes have adaptative responses to help buffer against deleterious mechanical force and thus reduce injury. However, these responses remain largely unknown. Here, using the Drosophila model, we show the mechanosensor Cheerio (dFilamin) provides a key protective role in nephrocytes. We found expression of an activated mechanosensitive variant of Cheerio rescued filtration function and induced compensatory and hypertrophic growth in nephrocytes depleted of the nephrocyte diaphragm proteins Sns or Duf. Delineating the protective pathway downstream of Cheerio we found repression of the Hippo pathway induces nephrocyte hypertrophy, whereas Hippo activation reversed the Cheerio-mediated hypertrophy. Furthermore, we find Yorkie was activated upon expression of active Cheerio. Taken together, our data suggest that Cheerio acts via the Hippo pathway to induce hypertrophic growth, as a protective response in abnormal nephrocytes.
Discrete reflexivity in function spaces

We study systematically when Cp(X) has a topological property P if Cp(X) is discretely P , i.e., the set D has P for every discrete subspace D ⊂ Cp(X). We prove that it is independent of ZFC whether discrete metrizability of Cp(X) implies its metrizability for a compact space X. We show that it is consistent with ZFC that countable tightness and Lindelöf Σ-property are not discretely reflexive in spaces Cp(X). It is also established that a space X must be countable and discrete if Cp(X) is discretely Čech-complete. If Cp(X) is discretely σ-compact then X has to be finite.
Fabrication and in vitro evaluation of 3D printed porous silicate substituted calcium phosphate scaffolds for bone tissue engineering

Silicate‐substituted calcium phosphate (Si‐CaP) ceramics, alternative materials for autogenous bone grafting, exhibit excellent osteoinductivity, osteoconductivity, biocompatibility, and biodegradability; thus, they have been widely used for treating bone defects. However, the limited control over the spatial structure and weak mechanical properties of conventional Si‐CaP ceramics hinder their wide application. Here, we used digital light processing (DLP) printing technology to fabricate a novel porous 3D printed Si‐CaP scaffold to enhance the scaffold properties. Scanning electron microscopy, compression tests, and computational fluid dynamics simulations of the 3D printed Si‐CaP scaffolds revealed a uniform spatial structure, appropriate mechanical properties, and effective interior permeability. Furthermore, compared to Si‐CaP groups, 3D printed Si‐CaP groups exhibited sustained release of silicon (Si), calcium (Ca), and phosphorus (P) ions. Furthermore, 3D printed Si‐CaP groups had more comprehensive and persistent osteogenic effects due to increased osteogenic factor expression and calcium deposition. Our results show that the 3D printed Si‐CaP scaffold successfully improved bone marrow mesenchymal stem cells (BMSCs) adhesion, proliferation, and osteogenic differentiation and possessed a distinct apatite mineralization ability. Overall, with the help of DLP printing technology, Si‐CaP ceramic materials facilitate the fabrication of ideal bone tissue engineering scaffolds with essential elements, providing a promising approach for bone regeneration.
Obstructive sleep apnea and anatomical structures of the nasomaxillary complex in adolescents

The aim of the present study was to reveal the associations between skeletal and soft tissue features of the nasomaxillary complex and development and severity of obstructive sleep apnea (OSA) in adolescents. A total of 100 adolescents (mean age, 14.9 ± 1.4 years; age range, 13–17 years) were enrolled. All participants underwent full-night polysomnography and had an assessment of size and position of the tongue, tonsillar size, body mass index (BMI), and circumference of the waist, neck, and hip. The skeletal features of the nasomaxillary complex, including the zygomatic arch width, nasal cavity width, nasal base width, intercanine width, intermolar width, maxillary dental arch length, palatal vault angle, palatal depth, and SNA were measured on the three-dimensional images constructed with computed tomography data. Participants with an apnea and hypopnea index (AHI) of lower than 5 (AHI ≤ 5) were classified as control and participants while those with an AHI of greater than 5 were classified as OSA group. Each variable with a significant outcome in the independent T-test and age and sex factors were integrated into the multivariate linear regression and the dependent variable was AHI. There were significant differences in the BMI and hip circumference between two groups. The width of nasal base, palatal vault angle and SNA also showed significant differences between groups. The results from multivariate linear regression demonstrated that the BMI, width of the nasal base, and SNA showed significant contributions to the severity of OSA in adolescents. The features of the nasomaxillary complex seemed to have significant influences on development and severity of OSA.
Quality control validation for a veterinary laboratory network of six Sysmex XT‐2000iV hematology analyzers

Abstract Background Quality control (QC) validation is an important step in the laboratory harmonization process. This includes the application of statistical QC requirements, procedures, and control rules to identify and maintain ongoing stable analytical performance. This provides confidence in the production of patient results that are suitable for clinical interpretation across a network of veterinary laboratories. Objectives To determine that a higher probability of error detection (P ed) and lower probability of false rejection (P fr) using a simple control rule and one level of quality control material (QCM) could be achieved using observed analytical performance than by using the manufacturer's acceptable ranges for QCM on the Sysmex XT‐2000iV hematology analyzers for veterinary use. We also determined whether Westgard Sigma Rules could be sufficient to monitor and maintain a sufficiently high level of analytical performance to support harmonization. Methods EZRules3 was used to investigate candidate QC rules and determine the P ed and P fr of manufacturer's acceptable limits and also analyzer‐specific observed analytical performance for each of the six Sysmex analyzers within our laboratory system using the American Society of Veterinary Clinical Pathology (ASVCP)‐recommended or internal expert opinion quality goals (expressed as total allowable error, TEa) as the quality requirement. The internal expert quality goals were generated by consensus of the Quality, Education, Planning, and Implementation (QEPI) group comprised of five clinical pathologists and seven laboratory technicians and managers. Sigma metrics, which are a useful monitoring tool and can be used in conjunction with Westgard Sigma Rules, were also calculated. Results The QC validation using the manufacturer's acceptable limits for analyzer 1 showed only 3/10 measurands reached acceptable P ed for veterinary laboratories (>0.85). For QC validation based on observed analyzer performance, the P ed was >0.94 using a 1‐2.5s QC rule for the majority of observations (57/60) across the group of analyzers at the recommended TEa. We found little variation in P fr between manufacturer acceptable limits and individual analyzer observed performance as this is a characteristic of the rule used, not the analyzer performance. Conclusions An improved probability of error detection and probability of false rejection using a 1‐2.5s QC rule for individual analyzer QC was achieved compared with the use of the manufacturers' acceptable limits for hematology in veterinary laboratories. A validated QC rule (1‐2.5s) in conjunction with sigma metrics (>5.5), desirable bias, and desirable CV based on biologic variation was successful to evaluate stable analytical performance supporting continued harmonization across the network of analyzers.
Kinetics, Products, and Brown Carbon Formation by Aqueous-Phase Reactions of Glycolaldehyde with Atmospheric Amines and Ammonium Sulfate

Glycolaldehyde (GAld) is a C2 water-soluble aldehyde produced during the atmospheric oxidation of isoprene and many other species and is commonly found in cloudwater. Previous work has established that glycolaldehyde evaporates more readily from drying aerosol droplets containing ammonium sulfate (AS) than does glyoxal, methylglyoxal, or hydroxyacetone, which implies that it does not oligomerize as quickly as these other species. Here, we report NMR measurements of glycolaldehyde’s aqueous-phase reactions with AS, methylamine, and glycine. Reaction rate constants are smaller than those of respective glyoxal and methylglyoxal reactions in the pH range of 3–6. In follow-up cloud chamber experiments, deliquesced glycine and AS seed particles were found to take up glycolaldehyde and methylamine and form brown carbon. At very high relative humidity, these changes were more than 2 orders of magnitude faster than predicted by our bulk liquid NMR kinetics measurements, suggesting that reactions involving surface-active species at crowded air–water interfaces may play an important role. The high-resolution liquid chromatography–electrospray ionization–mass spectrometric analysis of filter extracts of unprocessed AS + GAld seed particles identified sugar-like C6 and C12 GAld oligomers, including proposed product 3-deoxyglucosone, with and without modification by reactions with ammonia to diimine and imidazole forms. Chamber exposure to methylamine gas, cloud processing, and simulated sunlight increased the incorporation of both ammonia and methylamine into oligomers. Many C4–C16 imidazole derivatives were detected in an extract of chamber-exposed aerosol along with a predominance of N-derivatized C6 and C12 glycolaldehyde oligomers, suggesting that GAld is capable of forming brown carbon SOA.
Iterative Evaluation of a Driver Assistant Client in ICT-Systems in the Context of Urban Logistics and Electric Vehicles

—Information and communication technology (ICT) systems for electric vehicles (EVs), which support planning, monitoring and analysing urban area logistics can become complex and difﬁcult to use. In Smart City Logistik (SCL) project a driver assistant client (DAC) was developed to help to overcome fears, limited information and uncertainty in the context of urban logistics. To evaluate the users’ needs and intentions an iterative and open approach was designed and consequently used. Triangulation helped to get the best possible insights out of each of three phases of development, and the ﬁndings were used to improve the DAC. A lot of uncertainty accompanied the beginning of the project, so qualitative information was gathered to understand how the drivers work routines look like and which attitudes towards new technologies prevailed. A more quantitative approach helped to collect a broad range of opinions on speciﬁc usability topics before a ﬁnal simulated system setup contributes to ask a wide variety of users their experiences. This agile and iterative approach helped to identify important aspects while designing the DAC and compare different solutions, e.g., regarding necessary functionalities, menu-structure, font, button-size, and other parameters. The implementation of these ﬁndings enabled the project partners to develop a broadly accepted user interface and system that will be used in electric vehicles in urban logistics.
LTβR overexpression promotes plasma cell accumulation

Multiple myeloma (MM), a malignancy of plasma cells (PCs), has diverse genetic underpinnings and in rare cases these include amplification of the lymphotoxin b receptor (Ltbr) locus. LTβR has well defined roles in supporting lymphoid tissue development and function through actions in stromal and myeloid cells, but whether it is functional in PCs is unknown. Here we showed that Ltbr mRNA was upregulated in mouse PCs compared to follicular B cells, but deficiency in the receptor did not cause a reduction in PC responses to a T-dependent or T-independent immunogen. However, LTβR overexpression (OE) enhanced PC formation in vitro after LPS or anti-CD40 stimulation. In vivo, LTβR OE led to increased antigen-specific splenic and bone marrow (BM) plasma cells responses. LTβR OE PCs had increased expression of Nfkb2 and of the NF-kB target genes Bcl2 and Mcl1, factors involved in the formation of long-lived BM PCs. Our findings suggest a pathway by which Ltbr gene amplifications may contribute to MM development through increased NF-kB activity and induction of an anti-apoptotic transcriptional program.
Plasticity in Photoperiodism: Drosophila montana Females Have a Life-Long Ability to Switch From Reproduction to Diapause

Photoperiodic reproductive diapause is an essential part of female life cycle in several insect species living on high latitudes, where overwintering in reproductive stage involves high risks for survival and progeny production. The sensitive period (SP), during which photoperiodic cues can trigger the switch from direct development to diapause, can last from a few hours or days after emergence to the entire life span of females. Moreover, in some species, sexually mature females can enter post-reproductive diapause as a response to decreasing day length and/or temperature. We studied the duration of SP for diapause induction and the females’ ability to enter post-reproductive diapause at short day lengths in Drosophila montana strains from different latitudes in Europe, North America, and Japan. Our study shows that the females of this species have a life-long SP and that they retain an ability to switch between reproduction and diapause as a response to back-and-forth changes in day length for at least 3 months. D. montana strains from different latitudes showed high variation in females’ ability to enter post-reproductive diapause; females of the southern strains generally requiring longer time and/or lower temperature to enter this stage than those of the northern strains. Moreover, the proportion of females that switched to post-reproductive diapause in 3 weeks in short day conditions at 16 °C showed positive correlation with the critical day length (CDL) for diapause induction and the latitudinal and continental origin of the strains. Life-long SP increases females’ flexibility to respond to short-term changes in environmental conditions and enables reproducing females to switch to post-reproductive diapause when the days get shorter and colder toward the autumn. This ability can play a major role in species phenology and should be taken into account in theoretical and empirical studies on insect adaptation to seasonal variation.
sEMG-Triggered Fast Assistance Strategy for a Pneumatic Back Support Exoskeleton

To prevent lower back pain (LBP) in the industrial workplace, various powered back support exoskeletons (BSEs) have been developed. However, conventional kinematics-triggered assistance (KA) strategies induce latency, degrading assistance efficiency. Therefore, we proposed and experimentally evaluated a surface electromyography (sEMG)-triggered assistance (EA) strategy. Nine healthy subjects participated in the lifting experiments: 1) external loads test, 2) extra latency test, and 3) repetitive lifting test. In the external loads test, subject performed lifting with four different external loads (0 kg, 7.5 kg, 15 kg, and 22.5 kg). The assistance was triggered earlier by EA compared to KA from 114 ms to 202 ms, 163 ms to 269 ms for squat and stoop lifting respectively, as external loads increased from 0 kg to 22.5 kg. In the extra latency test, the effects of extra latency (manual switch, 0 ms, 100 ms and 200 ms) in EA on muscle activities were investigated. Muscle activities were minimized in the fast assistance (0 ms and 100 ms) condition and increased with extra latency. In the repetitive lifting test, the EA strategy significantly reduced L1 muscle fatigue by 70.4% in stoop lifting, compared to KA strategy. Based on the experimental results, we concluded that fast assistance triggered by sEMG improved assistance efficiency in BSE and was particularly beneficial in heavy external loads situations. The proposed assistive strategy can be used to prevent LBP by reducing back muscle fatigue and is easily applicable to various industrial exoskeleton applications.
The Environment, Decoherence and the Transition from Quantum to Classical

Quantum mechanics works exceedingly well in all practical applications. No example of conflict between its predictions and experiment is known. Without quantum physics we could not explain the behavior of solids, the structure and function of DNA, the color of the stars, the action of lasers or the properties of superfluids. Yet well over half a century after its inception, the debate about the relation of quantum mechanics to the familiar physical world continues. How can a theory that can account ith precision for everything we can measure still be deemed lacking?
Factor structure and measurement invariance of post-concussion symptom ratings on the Health and Behaviour Inventory across time, raters, and groups: An A-CAP study

Abstract Objectives: To validate the two-factor structure (i.e., cognitive and somatic) of the Health and Behaviour Inventory (HBI), a widely used post-concussive symptom (PCS) rating scale, through factor analyses using bifactor and correlated factor models and by examining measurement invariance (MI). Methods: PCS ratings were obtained from children aged 8–16.99 years, who presented to the emergency department with concussion (n = 565) or orthopedic injury (OI) (n = 289), and their parents, at 10-days, 3-months, and 6-months post-injury. Item-level HBI ratings were analyzed separately for parents and children using exploratory and confirmatory factor analyses (CFAs). Bifactor and correlated models were compared using various fit indices and tested for MI across time post-injury, raters (parent vs. child), and groups (concussion vs. OI). Results: CFAs showed good fit for both a three-factor bifactor model, consisting of a general factor with two subfactors (i.e., cognitive and somatic), and a correlated two-factor model with cognitive and somatic factors, at all time points for both raters. Some results suggested the possibility of a third factor involving fatigue. All models demonstrated strict invariance across raters and time. Group comparisons showed at least strong or strict invariance. Conclusions: The findings support the two symptom dimensions measured by the HBI. The three-factor bifactor model showed the best fit, suggesting that ratings on the HBI also can be captured by a general factor. Both correlated and bifactor models showed substantial MI. The results provide further validation of the HBI, supporting its use in childhood concussion research and clinical practice.
Complete Genome Sequence of the Cluster P Mycobacteriophage Phegasus

We characterized the complete genome of the cluster P mycobacteriophage Phegasus. Its 47.5-kb genome contains 81 protein-coding genes, 36 of which could be assigned a putative function. Phegasus is most closely related to two subcluster P1 bacteriophages, Mangethe and Majeke, with an average nucleotide identity of 99.63% each. ABSTRACT We characterized the complete genome of the cluster P mycobacteriophage Phegasus. Its 47.5-kb genome contains 81 protein-coding genes, 36 of which could be assigned a putative function. Phegasus is most closely related to two subcluster P1 bacteriophages, Mangethe and Majeke, with an average nucleotide identity of 99.63% each.
Electronic consultation in correctional facilities worldwide: a scoping review

Objective To provide an overview of the use of and evidence for eConsult in correctional facilities worldwide. Design Scoping review. Data sources Three academic databases (MEDLINE, Embase and CINAHL) were searched to identify papers published between 1990 and 2020 that presented data on eConsult use in correctional facilities. The grey literature was also searched for any resources that discussed eConsult use in correctional facilities. Articles and resources were excluded if they discussed synchronous, patient-to-provider or unsecure communication. The reference lists of included articles were also hand searched. Results Of the 226 records retrieved from the academic literature search and 595 from the grey literature search, 22 were included in the review. Most study populations included adult male offenders in a variety of correctional environments. These resources identified 13 unique eConsult services in six countries. Six of these services involved multiple medical specialties, while the remaining services were single specialty. The available evidence was organised into five identified themes: feasibility, cost-effectiveness, access to care, provider satisfaction and clinical impact. Conclusions This study identified evidence that the use of eConsult in correctional facilities is beneficial and avoids unnecessary transportation of offenders outside of the facilities. It is feasible, cost-effective, increases access to care, has an impact on clinical care and has high provider satisfaction. Some gaps in the literature remain, and we suggest further research on patient satisfaction, enablers and barriers to implementation, and women, youth and transgender populations in this setting to inform service providers and stakeholders. Despite some gaps, eConsult is evidently an important tool to provide timely, high-quality care to offenders.
A Study on Imputation using Adjusted Cohen Method

Many studies have been done to develop procedures to deal with missing values. Most common method is to reassign the other values to the missing data. The purpose of our study is to suggest adjusted Cohen methods and to compare the efficiency of them with other methods through a simulation study. The adjusted Cohen methods use an auxiliary variable to arrange ranking of the variable with missing values. It leads to a reduced mean square error(MSE) compared with the Cohen method.