Miracle-dz/UniFAR-FTUs huggingface dataset:
A Review of Dengue Fever with Special Reference to Malaysia

Introduction: Dengue is the most prevalent viral mosquito-borne disease, with over 2.5 billion humans at risk given its endemicity in more than 100 countries. Globally, 50-100 million cases of dengue occur annually, with approximately 0.7% resulting in dengue haemorrhagic fever (DHF), and 22,000 deaths. In 2017, there were 83,849 reported cases of dengue fever in endemic under-reported Malaysia, with 177 deaths. One of our authors (Verasingam K 2006) here, earlier published an article in the Med J Malaysia titled “Dengue Fever in Malaysia: Time for Review? ” This Article is meant as a sequel. Method: The Authors here narrate from their own personal-experiences, as well as from reviewing existing-literature. Results and Conclusion: Clinical Management has brought about vast improvements in mortality and morbidity. Similarly, great advancements in Laboratory Diagnostics. Prevention and Control methods have been desiring of greater achievements, but also show greater promise with Newer Insecticides, Innovative Methods and Vaccines. Dengue Fever would very likely become near-eradicated just like all other vaccine-preventable diseases, once comprehensive mass-vaccination programmes are available globally, using safe and very-effective tetravalent-vaccines soon to be available.
Prime Fermat numbers and maximum determinant matrix conjecture

Prime Fermat numbers and maximum determinant matrix conjecture N. A. Balonina, Dr. Sc. , Tech., Professor, orcid.org/0000-0001-7338-4920, korbendfs@mail.ru M. B. Sergeeva, Dr. Sc. , Tech., Professor, orcid.org/0000-0002-3845-9277 A. A. Vostricova, PhD, Tech., Associate Professor, orcid.org/0000-0002-8513-368 aSaint-Petersburg State University of Aerospaсe Instrumentation, 67, B. Morskaya St., 190000, Saint-Petersburg, Russian Federation
Un virus encore plus géant que les autres

biology. Nature 2002; 420: 629-35. 3. Burridge K, Wennerberg K. Rho and Rac take center stage. Cell 2004; 116: 167-79. 4. West MA, Wallin RP, Matthews SP, et al. Enhanced dendritic cell antigen capture via toll-like receptor-induced actin remodeling. Science 2004; 305: 1153-7. 5. Benvenuti F, Hugues S, Walmsley M, et al. Requirement of Rac1 and Rac2 expression by mature dendritic cells for T cell priming. Science 2004; 305: 1150-3. 6. West MA, Antoniou AN, Prescott AR, et al. Membrane ruffling, macropinocytosis and antigen presentation in the absence of gelsolin in murine dendritic cells. Eur J Immunol 1999; 29: 3450-5. 7. Burns S, Thrasher AJ, Blundell MP, et al. Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation. Blood 2001; 98: 1142-9.
Effects of elevated centrifugal field on the Nitella cell and postcentrifugation patterns of its cytoplasmic streaming and chloroplast files

Two stage centrifugation (250 × g and 6, 000 × g) was used to sediment organelles in layers in Nitella "leaf" cells. The earliest noticeable recovery of cytoplasmic streaming was minute and localized in the endoplasm adjacent to the chloroplast layer. This flow gradually expanded; within a few hours it covered the entire cellular space and showed an abnormal streaming pattern. At that time, chloroplasts still remained packed at the centrifugal end of the cell, and bundles of microfilaments were aligned along the direction of flow in the chloroplast-free area. The abnormal patterns observed were whirls, loops or flow perpendicular to the direction of normal streaming. Analysis of the angular velocities in the whirls indicated that each was an independent piece of rotating endoplasm, as reported by Kamitsubo (8, 9). Approximately one week later, chloroplasts began to reappear in the stripped area, and within 1 to 2 weeks the entire cell was covered with chloro-plasts exactly aligned along the direction of the reconstituted, abnormal flow. This suggests that chloroplast alignment can be governed by the direction of streaming. Cells with abnormal streaming pattrens survived for more than 3 months with no change in pattern. New cells initiated from the centrifuged cells, however, had normal streaming patterns and chloroplast alignments.
Identification of Regulatory Role of KRAB Zinc Finger Protein ZNF 350 and Enolase-1 in RE-IIBP Mediated Transcriptional Repression

Abstract － One of the WHSC1/MMSET/NSD2 variant RE-IIBP is a histone H3-K27 methyltransferase with transcriptional repression activity. Overexpression of RE-IIBP in various types of leukemia suggests it’s role in leukemogenesis. Here we identify two proteins, KRAB zinc finger protein ZNF 350 and enolase-1 as RE-IIBP interacting proteins by yeast two-hybrid screening and confirmed direct interaction in vivo and in vitro . Both proteins have been known for their role in transcriptional repression. Reporter assays using transient transfection demonstrated that both ZNF 350 and enolase-1 proteins synergistically repressed transcription with RE-IIBP, respectively. These results indicate both proteins have roles in RE-IIBP mediated tran-scriptional repression by involving co-repressor complex.Keywords: RE-IIBP, Yeast two-hybrid screening, ZNF 350, Enolase-1 INTRODUCTION In all eukaryotes, the histone modification plays an im-portant role in the transcriptional regulation, development, and DNA repair (Kouzarides, 2007). The methylation of histone tails at specific residues are five lysines within K4, K9, K27, K36, K79 on H3 and one lysine K20 within histone H4 that have been methylated by specific histone methyl-transferase (HMTase) (Sims
Potential of algal metabolites for the development of broad‐spectrum antiviral therapeutics: Possible implications in COVID‐19 therapy

Covid‐19 pandemic severely affected human health worldwide. Till October 19, 2020, total confirmed patients of COVID‐19 are 39,944,882, whereas 1,111,998 people died across the globe. Till to date, we do not have any specific medicine and/or vaccine to treat COVID‐19; however, research is still going on at war footing. So far vaccine development is concerned, here it is noteworthy that till now three major variants (named A, B, and C) of severe acute respiratory syndrome‐coronavirus2 (SARS‐CoV‐2) have been recognized. Increased mutational rate and formation of new viral variants may increase the attrition rate of vaccines and/or candidate chemotherapies. Herbal remedies are chemical cocktails, thus open another avenue for effective antiviral therapeutics development. In fact, India is a large country, which is densely populated, but the overall severity of COVID‐19 per million populations is lesser than any other country of the world. One of the major reasons for the aforesaid difference is the use of herbal remedies by the Government of India as a preventive measure for COVID‐19. Therefore, the present review focuses on the epidemiology and molecular pathogenesis of COVID‐19 and explores algal metabolites for their antiviral properties.
Supercade: A Visual History of the Videogame Age 1971-1984

From the Publisher: "From Pong to Pac-Man, Asteroids to Zaxxon - more than fifty million people around the world have come of age within the electronic flux of videogames, their subconscious forever etched with images projected from arcade and home videogame systems. " From the first interactive blips of electronic light at Brookhaven National Labs and the creation of Spacewar! at the Massachusetts Institute of Technology; to the invention of the TV Game Project and the myriad systems of Magnavox, Atari, Coleco, and Mattel that followed; through the rise of the Golden Age of videogames and forward into the imagination of millions, Supercade is the first book to illustrate and document the history, legacy, and visual language of the videogame phenomenon.
RANCANG BANGUN INTERNET OF THINGS SERVER DENGAN MENGGUNAKAN ACTIVEMQ ARTEMIS UNTUK MITIGASI BENCANA DI RASPBERRY PI 3

Indonesia merupakan negara kepulauan yang mempunyai lebih dari 13.000 pulau. Wilayahnya terletak di antara Samudera Hindia dan Samudera Pasifik dan dilewati oleh Pacific Ring of Fire sehingga banyak gunung berapi aktif. Berdasarkan letak geografis mempunyai potensi tsunami dan gempa bumi cukup tinggi. Diperlukan rencana penanggulangan bencana yang baik untuk menekan risiko yang bisa terjadi, salah satunya dengan mitigasi bencana. Mitigasi bencana adalah serangkaian upaya untuk mengurangi risiko bencana, baik melalui pembangunan fisik maupun penyadaran dan peningkatan kemampuan menghadapi ancaman bencana. Mitigasi bencana diperlukan untuk mengurangi dampak yang ditimbulkan terutama korban jiwa. Salah satunya dengan menggunakan sistem peringatan dini. Sistem peringatan dini terdiri dari 3 komponen utama yaitu sensor untuk mendapatkan nilai dari suatu lingkungan, controller untuk mengolah nilai yang diterima, dan aksi yang dilakukan berdasarkan hasil dari pengolahan. Untuk membuat sistem yang efektif diperlukan komunikasi yang memadai. Messaging queue digunakan oleh industri untuk komunikasi antar perangkat lunak, perangkat keras, dan embedded system. Penelitian berfokus pada penggunaan ActiveMQ Artemis sebagai messaging queue sebagai server untuk komunikasi dengan internet of things (IoT). Keunggulan ActiveMQ Artemis dapat dijalankan di Raspberry Pi 3 dengan sedikit modifikasi. Hasil penelitian membuktikan bahwa ActiveMQ Artemis dapat digunakan untuk komunikasi IoT pada simulasi sistem mitigasi bencana.
Animal Welfare: Limping Towards Eden: A Practical Approach to Redressing the Problem of Our Dominion Over the Animals

Preface. Acknowledgements. 1. Introduction: Facts and Values. 2. Challenge and Response. 3. Sentience, Sense and Sufferering. 4. Husbandry and Welfare on the Farm: Assessment and Assurance. 5. Animals for Food: Indutrialized Farming, Pigs and Poultry. 6. Animals for Food: Cattle and Other Ruminants. 7. Animals for Food: Handling, Transport and Slaughter. 8. Animals for Science and Biotechnology. 9. Animals for Sport. 10. Animals as Pets. 11. Limping Towards Eden: Stepping Stones. Further Reading. Index.
Optimisation of Substrate and Growth Conditions of Retinal Pigment Epithelial Cells Destined for Transplantation

Retinal Pigment Epithelium (RPE) transplantation efforts have been ongoing for three decades . To this day no feasible cure exists for diseases such as age-related macular degeneration (AMD). The key to treatment of AMD is to replenish the RPE. Establishing cells that accurately represent their native tissue is a considerable challenge. RPE cells undergo de-differentiation in culture losing important characteristics after repeated passage. Furthermore, RPE cells are anchorage-dependant and require a substrate for survival. Attempts at replenishing the RPE using suspensions have been met with scepticism due to the high degree of apoptosis. ARPE-19, a human RPE line, retains several phenotypic characteristics of primary RPE although current passages have lost some established features. A variety of polymers with diverse chemistries, specialised coatings, and optimised media were tested to optimally grow ARPE-19 cells. Using information gathered from these experiments, optimal conditions were selected for Human Embryonic Stem Cell (HESC)-derived RPE cells. Cell/substrate composites were transplanted in pigs to validate their efficacy. Differentiation of ARPE-19 cells was enhanced by utilising a superior substrate, polyester filter, together with an optimal growth medium containing pyruvate. HESCderived RPE grown in optimal conditions developed differentiation characteristics identical to native RPE. This was assessed by morphology, immunohistochemical profile, trans-epithelial resistance, electron-microscopy, and growth factor secretion. A higher porosity version of this filter supported growth and differentiation of HESC-RPE and this was chosen as the basis for a transplantation project due to its good permeability. Transplanted HESC-RPE/polyester composites survived surgical delivery in a pig model and were eventually chosen for a phase I clinical trial. Conclusion: This thesis investigated optimal conditions for the human RPE line ARPE- 19. Optimal growth conditions were then applied to HESC-RPE cells which achieved a high level of differentiation. Composites were transplanted in pigs and led to their selection for use in clinical trials.
Regulatory Science: Mathematical vs. Statistical Models

Natural resource companies do not object to environmental regulations that are consistent and support predictability. Consistency and predictability are critical for decision making under conditions of uncertainty. Natural ecosystems are inherently variable across a broad range of temporal and spatial scales; climate change, drought, and societal desires for sustainability make people more aware of this variability. The science used for development and enforcement of environmental regulations has not kept pace with developments in ecological theory and the analytical tools capable of describing, characterizing, classifying, and predicting natural ecosystems as well as distinguishing natural variability from anthropogenic changes.Because natural resource industries (agriculture, energy, mining) provide the base for all economic and societal activities it is critical that environmental statutes and regulations be regularly updated to use the most technically sound and legally defensible scientific knowledge and tools.Mathematical models were the tools of choice when environmental statutes and regulations were introduced, perhaps because they were successfully applied to static components of the built environment such as buildings and bridges. While their limitations for highly variable natural ecosystems were accepted then, there is now no benefit to not replacing them with statistical models.This paper describes limitations in policy and regulatory decision-making based on mathematical models and explains how the appropriate statistical models avoid the subjectivity and rigidity of the former. Changing the basis of determining and justifying policy and environmental regulations is consistent with the concepts of regulatory science applied to human health.https://doi.org/10.21423/jrs-v04n04p010 (DOI assigned 5/14/2019)
Interdisciplinary CS1 Course for Non-Majors: The Case of Graduate Psychology Students

The objective of interdisciplinary education is to teach multiple domains, so that the students can solve integrative problems that require knowledge and skills from these domains. Interdisciplinary programs, in which computer science is combined with other domains, range from bioinformatics to computational linguistics and data science. Data science is an interdisciplinary field that integrates computer science, mathematics, statistics, and the domain knowledge of the data involved. It is a relevant research skill for many disciplines, including social sciences. Specifically, graduate psychology students who wish to learn data science have already mastered the psychology domain knowledge and their statistics background is advanced. They lack, however, the computer science knowledge required for meaningfully handling data science. The problem is, however, that there is no CS1 course that fits graduate psychology students who study data science: On the one hand, the current CS1 courses do not suit social science students; on the other hand, CS1 courses for non-majors usually do not meet the required level of computer science needed for data science. Accordingly, to close this gap, we designed a new CS1 course – an interdisciplinary Introduction to Computer Science for Psychological Science course – that a) suits social science students and b) meets the required computer science level for data science. We evaluated the course by examining students’ learning outcomes, reflections, and responses on questionnaires. Based on the data analysis according to the self-determination motivation theory, it is concluded that the course design enabled to achieve the course’s targets.
A CAD System for Diagramming Origami with Prediction of Folding Processes

In recent years, many methods for designing origami pieces based on mathematical theories have been developed. One of the most powerful approaches has been implemented in the software system TreeMaker [Lang 06]. Although origami pieces designed using this software are restricted to a particular structure referred to as a uniaxial origami base, many complex and realistic works of art have been created. Describing the sequence of folds used to create a complex work is difficult. However, traditional origami diagrams, namely, a sequence of step-by-step illustrations, are still used, even today. One problem in using such diagrams is that drawing diagrams manually is time consuming; as an origami piece becomes more complex, the cost for drawing the diagrams increases. Traditional origami pieces, such as a crane, are folded by approximately a dozen steps. But, many recent complex pieces need more than 100 steps, thus requiring more than 100 diagrams each to explain how to fold them. To avoid this cost, a crease pattern can sometimes be used instead of a diagram. A crease pattern consists of line segments that show the crease lines that appear on the paper when an origami piece is unfolded. Although drawing crease patterns is much easier than drawing diagrams, folding from crease patterns is more difficult for nonexperts because crease patterns do not contain any proce-
Composition and Diversity of LTR Retrotransposons in the Coffee Leaf Rust Genome (Hemileia vastatrix)

Coffee leaf rust is the most damaging disease for coffee cultivation around the world. It is caused by a fungal pathogen, Hemileia vastatrix (Hva), belonging to the phylum Basidiomycota. Coffee leaf rust causes significant yield losses and increases costs related to its control, with evaluated losses of USD 1–2 billion annually. It attacks both the cultivated coffee species Coffea canephora (Robusta coffee) and Coffea arabica (Arabica coffee). New races, or pathotypes, are constantly emerging with increased virulence, suggesting a rapid evolution of the pathogen. Previous genetic and genomic studies have indicated a limited nucleotide diversity of Hva despite a high genetic diversity and large genome size estimated to be ~800 Mb, with a high content of repeated sequences (>74%). Despite several genomic resources and the release of a recent partial genome sequence, the diversity of these repeated sequences and how they may impact the evolution of the leaf rust genome have not been investigated in detail. In an attempt to characterize the transposable elements within the Hva genomes, we report here new lineages of long terminal repeat (LTR) retrotransposons, called CO-HUI, Soroa, and Baco, which are classified into Gypsy, and and Labe and Mapi, which are classified as Copia. The CO-HUI and Soroa elements represent the main part of all Hva transposable elements, as well as approximately 37% of the available genome assemblies. Mapi and CO-HUI are the main expressed families in RNA-seq data. Although Soroa is the lineage showing more insertions into exons and genes, Mapi seems to be more frequently involved in co-expression with genes. All these new families are also present in the Pucciniales, suggesting that they dynamically participate in their genome evolution.
On conservation of probability and the Feller property

It is known that any smooth, nondegenerate, second-order elliptic operator on a manifold (dimension? 2) has the form Δ + B, where B is a vector field and Δ is the Laplace-Beltrami operator under some Riemannian metric on the manifold. In this paper we give several conditions on the Ricci curvature Ric - ⊇ s B associated with the operator Δ + B to ensure that the diffusion semigroup generated by Δ + B conserves probability and possesses the Feller property.
Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Simple Summary To enhance the antibiotic treatment during the realisation of elective caesarean sections (CSs), a better knowledge of the bacterial composition of the surgical site is required. This study involved 13 cows of a previous dataset of 76 animals. Bacteriology was performed on swabs sampled from visceral and parietal peritoneum during the CS. DNA sequencing was performed on six samples chosen randomly among the culture-positive swabs and seven among the culture-negative swabs. No difference was observed between the microbiota composition of the samples positive or negative to bacterial culture. In fact, a high amount of bacterial DNA was identified in all surgical sites of elective CSs, and the most identified species was Mycoplasma wenyonii. Furthermore, the bacteria cultured were not the dominant species, since most of them were not identified by amplicon sequencing in the same sample in which they were cultured. It is assumed that the most identified bacterial DNA is due to the haematogenous contamination of the surgical site. To understand the role (protector vs. harmful) of these bacteria in the occurrence of post-operative complications and the effect of antibiotic treatment (protector vs. harmful) on the microbiota, further studies are required. Abstract This study aimed to describe the bacterial composition of the surgical site during elective caesarean sections (CSs) using the 16Sr DNA amplicon sequencing performed in parallel to bacterial culture. The study involved 13 Belgian blue cows of a previous dataset of 76 animals. Bacteriology was performed on swabs sampled from visceral and parietal peritoneum during the CS. Amplicon sequencing was performed in six samples chosen randomly among the swabs positive for bacteriology and seven among the culture-negative swabs. A total of 2542 bacterial operational taxonomic units belonging to 567 genera were identified. The most often identified genus and species were Mycoplasma (44%) and Mycoplasma wenyonii (36%), respectively. Results showed no difference in microbiota composition between the culture-positive and -negative samples. However, a difference was observed between the bacteriology and amplicon sequencing results. Indeed, seven out of nine cultured strains were not identified by amplicon sequencing in the samples in which they were cultured. In contrast to bacteriology, amplicon sequencing unveiled the presence of bacterial DNA in all elective CSs. The most identified DNA is most likely derived from the haematogenous spread of bacteria to the surgical site. Furthermore, the cultured bacteria were not the dominant species in the sample from which they were cultured.
CtDNA’s prognostic value in patients with early-stage colorectal cancer after surgery: A meta-analysis and systematic review

Background: Circulating tumor DNA (ctDNA) positivity has been shown to suggest the presence of minimally residual tumor cells in numerous investigations. We aimed to assess the prognostic value of ctDNA positivity for recurrence-free survival in patients with early-stage colorectal cancer after radical surgery and following adjuvant chemotherapy. Methods: We systematically reviewed studies published in English until August 15, 2022, concerning ctDNA and tumor-node-metastasis I to III colorectal cancer after surgery, and quantified the correlation between ctDNA positivity and early-stage (tumor-node-metastasis stage I–III) colorectal cancer using meta-analysis methods. Results: In total, the meta-analysis comprised 1713 patients from 6 studies. Patients with ctDNA-positive colorectal cancer after surgery had a significantly higher risk of recurrence than patients with ctDNA-negative colorectal cancer (hazard ratio 4.64, 95% confidence interval 2.17–9.92, z = 3.96; P < .001). After adjuvant chemotherapy, patients who were ctDNA-positive had a significantly higher risk of recurrence than those who were ctDNA-negative (hazard ratio 7.27, 95% confidence interval 4.50–11.75, z = 8.1; P < .001). Conclusions: CtDNA positivity may potentially be a predictor for early-stage colorectal tumor recurrence following surgery and adjuvant chemotherapy.