Miracle-dz/UniFAR-FTUs huggingface dataset:
Constraints on Dark Matter Microphysics from the Milky Way Satellite Population

Alternatives to the cold, collisionless dark matter (DM) paradigm in which DM behaves as a collisional fluid generically suppress small-scale structure. Herein we use the observed population of Milky Way (MW) satellite galaxies to constrain the collisional nature of DM, focusing on DM–baryon scattering. We first derive conservative analytic upper limits on the velocity-independent DM–baryon scattering cross section by translating the upper bound on the lowest mass of halos inferred to host satellites into a characteristic cutoff scale in the linear matter power spectrum. We then confirm and improve these results through a detailed probabilistic inference of the MW satellite population that marginalizes over relevant astrophysical uncertainties. This yields 95% confidence upper limits on the DM–baryon scattering cross section of 6 × 10−30 cm2 (10−27 cm2) for DM particle masses mχ of 10 keV (10 GeV); these limits scale as mχ1/4 for mχ ≪ 1 GeV and mχ for mχ ≫ 1 GeV. This analysis improves upon cosmological bounds derived from cosmic-microwave-background anisotropy measurements by more than three orders of magnitude over a wide range of DM masses, excluding regions of parameter space previously unexplored by other methods, including direct-detection experiments. Our work reveals a mapping between DM–baryon scattering and other alternative DM models, and we discuss the implications of our results for warm and fuzzy DM scenarios.
Antigen I/II Participates in the Interactions of Streptococcus suis Serotype 9 With Phagocytes and the Development of Systemic Disease

Streptococcus suis is an important porcine bacterial pathogen and a zoonotic agent causing a variety of pathologies including sudden death, septic shock, and meningitis. Though serotype 2 is the most studied serotype due to its presence worldwide, serotype 9 is responsible for the greatest number of porcine cases in Spain, the Netherlands, and Germany. Regardless of its increasing importance, very few studies have investigated S. suis serotype 9 virulence factors and pathogenesis. Antigens I/II (AgI/II) are multimodal adhesion proteins implicated in host respiratory tract and oral cavity persistence of various pathogenic human streptococci. It was recently demonstrated that AgI/II is involved in various bacterial functions for serotype 9, participating in the initial steps of the pathogenesis of the infection. However, its contribution to the systemic infection remains unknown. As such, we evaluated herein the role of the S. suis serotype 9 AgI/II in the interactions with phagocytes and the development of systemic disease in a mouse model of infection. Results demonstrated that the presence of AgI/II is important for the development of clinical systemic disease by promoting bacterial survival in blood possibly due to its effect on S. suis phagocytosis, as shown with macrophages and dendritic cells. Furthermore, AgI/II directly participates in dendritic cell activation and pro-inflammatory mediator production following recognition by the Toll-like receptor pathway, which may contribute to the exacerbated systemic inflammation responsible for host death. Taken together, this study demonstrates that the S. suis serotype 9 AgI/II is important for virulence during systemic infection and development of disease. In fact, this is the first study to describe a role of an AgI/II family member in systemic bacterial disease.
A Polyamidoamine Dendrimer-Based Electrochemical Immunosensor for Label-Free Determination of Epithelial Cell Adhesion Molecule- Expressing Cancer Cells

A new electrochemical immunosensor for cancer cell detection based on a specific interaction between the metastasis-related antigen of epithelial cell adhesion molecule (EpCAM) on the cell membrane and its monoclonal antibody (Anti-EpCAM) immobilized on a gold electrode has been developed. The amino-terminated polyamidoamine dendrimer (G6 PAMAM) was first covalently attached to the 3-mercaptopropionic acid (MPA)-functionalized gold electrode to obtain a thin film, and then completely carboxylated by succinic anhydride (SA). Next, the Anti-EpCAM was covalently bound with the G6 PAMAM to obtain a stable recognition layer. In the presence of the EpCAM expressing hepatocellular carcinomas cell line of HepG2, the specific immune recognition (Anti-EpCAM/EpCAM) led to an obvious change of the electron transfer ability. The properties of the layer-by-layer assembly process was examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The final determination of HepG2 cells was performed in the presence of the reversible [Fe(CN)6]3−/4− redox couple using impedance technique. Based on the advantages of PAMAM nanomaterial and immune reaction, a linear response to HepG2 cells ranging from 1 × 104 to 1 × 106 cells mL−1 with a calculated detection limit of 2.1 × 103 cells mL−1 was obtained. We expect this method can provide a potential tool for cancer cell monitoring and protein expression analysis.
Prepregnancy Habitual Intakes of Total, Supplemental, and Food Folate and Risk of Gestational Diabetes Mellitus: A Prospective Cohort Study

OBJECTIVE To identify novel modifiable risk factors of gestational diabetes mellitus (GDM) by examining the association between prepregnancy habitual folate intake and GDM risk. RESEARCH DESIGN AND METHODS The study included 14,553 women in the Nurses’ Health Study II who reported at least one singleton pregnancy between the 1991 and 2001 questionnaires. Prepregnancy intakes of total folate, supplemental folate, and food folate were assessed using a food frequency questionnaire administered every 4 years. Incident GDM was ascertained from a self-reported physician diagnosis. Relative risks (RRs) of GDM were estimated using log-binomial models, with adjustment for demographic, lifestyle, and dietary factors. RESULTS Over the study follow-up, 824 incident GDM cases were reported among 20,199 pregnancies. Women with adequate total folate intake (≥400 μg/day) had an RR of GDM of 0.83 (95% CI 0.72, 0,95, P = 0.007) compared with women with inadequate intake (<400 μg/day). This association was entirely driven by supplemental folate intake. The RRs of GDM for 1–399, 400–599, and ≥600 μg/day of supplemental folate intake were 0.83, 0.77, and 0.70, respectively, compared with no supplemental folate intake (Ptrend = 0.002). The association between supplemental folate intake and GDM risk largely persisted after additional adjustment for intake of multivitamins and other micronutrients, as well as among women who likely planned for the pregnancy. CONCLUSIONS Higher habitual intakes of supplemental folate before pregnancy were significantly associated with lower GDM risk. If confirmed, these findings indicate that prepregnancy folic acid supplementation could offer a novel and low-cost avenue to reduce GDM risk.
Evaluating the effects and benefits of cover crops in citrus orchards: a review

Cover crops are used in sustainable agricultural practices to improve soil quality and minimise the effect of climate change, water restrictions and drought. The South African citrus industry produces and exports a diverse range of citrus fruits; however, knowledge of cover crops in citrus orchards in South Africa is limited. This review focuses mostly on evaluating cover crop selection, effects on the soil microbial community, weed control, citrus yield and fruit quality. In terms of cover crop selection, legumes seem to be preferred over non-legume cover crops. The reviewed literature reported that legume cover crops increased nitrogen by 67 to 209 kg N ha−1 through the nitrogen fixation process. Additionally, the cultivation of legume cover crops substantially improved the cost savings of nitrogen fertiliser. The fruit yield improved by 7.6 to 64%, while fruit quality improved by 2.3 to 12.4% fruit weight and 2.4 to 5.8% Bx. This demonstrates that the benefits of cover crops to South African citrus growers include improved soil quality and fruit yields and decreased costs.
Effects of Long-Term Dietary Protein Restriction on Intestinal Morphology, Digestive Enzymes, Gut Hormones, and Colonic Microbiota in Pigs

Simple Summary In China, a shortage of protein resources is an important limiting factor to the economic benefit of pig production, and the use of protein-restriction diets balanced with amino acids is an effective strategy to save protein resources. However, long-term protein-restriction diets can impair the growth performance, and the reason is still unknown. This study is to investigate the response of gastrointestinal physiology and gut microbiota to the condition of long-term low-protein diet and to try to provide a theoretical foundation for better use of protein resources in swine production. Results showed that presented with moderate protein-restriction diets, pigs are able to adjust their absorption and consumption of nutrients to maintain growth performance; whereas extremely low-protein diets suppress pigs’ appetite, impair intestinal morphology, decrease Lactobacillus and Streptococcus, and reduce energy expenditure. Thus, moderate reduction of dietary protein is more suitable for pig production than extremely low-protein diets supplemented with essential amino acids, and moderate protein-restriction diets can potentially increase protein utilization in pig production. Abstract Using protein-restriction diets becomes a potential strategy to save the dietary protein resources. However, the mechanism of low-protein diets influencing pigs’ growth performance is still controversial. This study aimed to investigate the effect of protein-restriction diets on gastrointestinal physiology and gut microbiota in pigs. Eighteen weaned piglets were randomly allocated to three groups with different dietary protein levels. After a 16-week trial, the results showed that feeding a low-protein diet to pigs impaired the epithelial morphology of duodenum and jejunum (p < 0.05) and reduced the concentration of many plasma hormones (p < 0.05), such as ghrelin, somatostatin, glucose-dependent insulin-tropic polypeptide, leptin, and gastrin. The relative abundance of Streptococcus and Lactobacillus in colon and microbiota metabolites was also decreased by extreme protein-restriction diets (p < 0.05). These findings suggested that long-term ingestion of a protein-restricted diet could impair intestinal morphology, suppress gut hormone secretion, and change the microbial community and fermentation metabolites in pigs, while the moderately low-protein diet had a minimal effect on gut function and did not impair growth performance.
Maternal Cardiovascular Risk Assessment 3-to-11 Years Postpartum in Relation to Previous Occurrence of Pregnancy-Related Complications

The aim of the present study was to assess the long-term outcomes of women 3-to-11 years postpartum in relation to the previous occurrence of pregnancy-related complications such as gestational hypertension (GH), preeclampsia (PE) and fetal growth restriction (FGR). Body mass index (BMI), waist circumference values, the average values of systolic (SBP) and diastolic (DBP) blood pressures and heart rate, total serum cholesterol levels, serum HDL (high-density lipoprotein) cholesterol levels, serum LDL (low-density lipoprotein) cholesterol levels, serum triglycerides levels, serum lipoprotein A levels, serum CRP (C-reactive protein) levels, plasma homocysteine levels, serum uric acid levels, individual and relative risks of having a heart attack or stroke over the next ten years were compared between groups (50 GH, 102 PE, 34 FGR and 90 normal pregnancies) and correlated with the severity of the disease with regard to clinical signs (25 PE without severe features, 77 PE with severe features), and delivery date (36 early PE, 66 late PE). The adjustment for potential covariates was made, where appropriate. At 3–11 years follow-up women with a history of GH, PE regardless of the severity of the disease and the delivery date, PE without severe features, PE with severe features, early PE, and late PE had higher BMI, waist circumferences, SBP, DBP, and predicted 10-year cardiovascular event risk when compared with women with a history of normotensive term pregnancy. In addition, increased serum levels of uric acid were found in patients previously affected with GH, PE regardless of the severity of the disease and the delivery date, PE with severe features, early PE, and late PE. Higher serum levels of lipoprotein A were found in patients previously affected with early PE. The receiver operating characteristic (ROC) curve analyses were able to identify a substantial proportion of women previously affected with GH or PE with a predisposition to later onset of cardiovascular diseases. Women with a history of GH and PE represent a risky group of patients that may benefit from implementation of early primary prevention strategies.
Prevalence of Anemia in Chinese Children and Adolescents and Its Associated Factors

In this study, we assessed the hemoglobin levels and anemia status of Chinese children and adolescents from the Chinese National Nutrition and Health Survey (CNNHS) in 2010–2012 and analyzed the factors associated with anemia. The hemoglobin concentration and prevalence of anemia for children and adolescents aged 6–17 years from both CNNHS 2010–2012 and CNNHS 2002 were analyzed. Multi-variable logistic regression analysis was used to assess the factors associated with anemia. The mean hemoglobin concentration increased among Chinese children and adolescents, from 135.2 ± 13.9g/L in CNNHS 2002 to 141.2 ± 15.8 g/L in CNNHS 2010–2012, with the prevalence of anemia decreasing from 12.6% to 6.6% (p < 0.0001). Anemia was specifically related to girls (p < 0.0001); children aged 6–8 years (p = 0.0175), 12–14 years (p = 0.0007), and 15–17 years (p < 0.0001); ordinary rural areas (p = 0.0009) and poor rural areas (p < 0.0001);spring (p < 0.0001), autumn (p < 0.0001), and winter (p < 0.0001);underweight individuals (p < 0.0001); and an annual average income per capita of less than 20,000 RMB (p < 0.0001). The prevalence of anemia in Chinese children and adolescents has improved significantly in comparison to 10 years prior; however, it remains a public health problem in this population. Further research is required to understand the determinants of iron status, which could then lead to strategies to alleviate iron deficiency for Chinese children and adolescents, especially for girls, those living in rural areas, underweight individuals, and those with a low family income.
Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage

Dengue virus (DENV) infection, the most common mosquito-transmitted viral infection, can cause a range of diseases from self-limiting dengue fever to life-threatening dengue hemorrhagic fever and shock syndrome. Thrombocytopenia is a major characteristic observed in both mild and severe dengue disease and is significantly correlated with the progression of dengue severity. Previous studies have shown that DENV nonstructural protein 1 (NS1), which can be secreted into patients’ blood, can stimulate immune cells via Toll-like receptor 4 (TLR4) and can cause endothelial leakage. However, it is unclear whether DENV NS1 can directly induce platelet activation or cause thrombocytopenia during DENV infection. In this study, we first demonstrated that DENV but not Zika virus cell culture supernatant could induce P-selectin expression and phosphatidylserine (PS) exposure in human platelets, both of which were abolished when NS1 was depleted from the DENV supernatant. Similar results were found using recombinant NS1 from all four serotypes of DENV, and those effects were blocked in the presence of anti-NS1 F(ab’)2, anti-TLR4 antibody, a TLR4 antagonist (Rhodobacter sphaeroides lipopolysaccharide, LPS-Rs) and a TLR4 signaling inhibitor (TAK242), but not polymyxin B (an LPS inhibitor). Moreover, the activation of platelets by DENV NS1 promoted subthreshold concentrations of adenosine diphosphate (ADP)-induced platelet aggregation and enhanced platelet adhesion to endothelial cells and phagocytosis by macrophages. Finally, we demonstrated that DENV-induced thrombocytopenia and hemorrhage were attenuated in TLR4 knockout and wild-type mice when NS1 was depleted from DENV supernatant. Taken together, these results suggest that the binding of DENV NS1 to TLR4 on platelets can trigger its activation, which may contribute to thrombocytopenia and hemorrhage during dengue infection.
Influence of Calcium on the Structure and Hydrophilic of CaTiO3 Coating on Titanium Implants

Calcium titanate (CaTiO3), a multi-metal oxide has received extensive attention in recent years, due to its unique structural features, high chemical stability, strong catalytic activity, inexpensiveness, low toxicity, and easy synthesis. In this work, we have focused our research on and investigated the influence of Ca weight on the microstructure and properties of CaTiO3 films. CaTiO3 films on TiO2 nanotube templates were synthesized using hydrothermal method at 200 oC for 24 h. The TiO2 template was synthesized by anodizing using a Ti plate. The synthesized materials were analyzed on their crystal phase, surface morphology, Raman characterization, surface roughness, and hydrophilic properties by X-ray diffraction (XRD), Raman, scanning electron microscopy (SEM), 3D optical Profilometer, and contact angle measurement. The synthesized film exhibited a morphological transformation from nanotube morphology to nanopillars. Notably, the hydrophilic properties and the surface roughness of the CaTiO3 films were altered after hydrothermal treatment of the TiO2 nanotube template. These findings could potentially lead to the development of highly efficient materials for use in biomedical implants.
EXCESSIVE USE OF MOBILE PHONES AND ITS PSYCHOLOGICAL IMPACT ON NURSING STUDENTS

As we advance into the digital era, mobile devices have become integral to our daily interactions. Nevertheless, an intensified engagement with these devices has prompted concerns about potential psychological implications among nursing students, influencing their scholastic achievements and personal wellness. The primary objectives of this study were to gauge the frequency and modalities of mobile phone utilization among nursing students, ascertain its effects on their sleep quality, and evaluate its interference with academic and work-related tasks. The study additionally aimed to discern the students' perceived dependency on mobile phones and its subsequent impact on interpersonal relationships. This study employed a descriptive cross-sectional methodology, focusing on the student population at People's Nursing School, LUMHS Jamshoro. A cohort of 162 students was enlisted through a non-probability convenience sampling technique. A meticulously crafted questionnaire served as the primary tool for data collection, capturing an array of participant experiences and viewpoints. A predominant segment of the participants comprised females from middle-income families, boasting an average age of 20.83 (Std. Dev = 1.34). A substantial proportion (58.6%) reported smartphone engagement of less than 6 hours daily. Elevated anxiety levels were observed, with a notable fraction attributing their stress to mobile phone usage. Approximately 32.7% posited that their sleep quality was compromised due to their phone habits. Moreover, 35.8% indicated strained relationships with friends and family due to increased mobile engagement. Several participants emphasized key concerns such as insomnia and profound feelings of isolation linked to heightened mobile usage. The data highlights an urgent need to address the multifaceted psychological and social ramifications of unrestrained mobile phone interactions among nursing students. While these devices usher in many conveniences, their unchecked usage could catalyze profound challenges, emphasizing the need for strategic interventions to safeguard prospective healthcare professionals' mental and emotional health.
Novel Circulating miRNA Signatures for Early Detection of Pancreatic Neoplasia

OBJECTIVES: Pancreatic ductal adenocarcinoma (PDAC) presents the lowest survival rate of all cancers because only 6% of patients reach five-year survival. Alterations in the expression of several microRNAs (miRNAs) occur in the tumor of PDAC and in preneoplastic lesions as the called intraductal papillary mucinous neoplasm (IPMN). Here, we aimed at identifying which miRNAs are significantly altered in liquid biopsies from patients with PDAC and IPMN to find new noninvasive biomarkers for early detection of PDAC. METHODS: We analyzed by real-time quantitative reverse transcription-PCR (qRT-PCR) the expression of 17 circulating miRNAs, previously found to be significantly overexpressed in tissue pancreatic neoplasms, in a set of 182 plasma samples (94 PDAC, 19 IPMN, 18 chronic pancreatitis, and 51 disease-free controls). Then, we analyzed CA19.9 levels in the same plasma set, and we assessed the diagnostic values of differentially expressed miRNAs, CA19.9, and all possible combinations. RESULTS: Of note, 16, 14, and 9 miRNAs were significantly increased in PDAC, IPMN, and chronic pancreatitis, respectively, compared with control plasmas. miR-21-5p, miR-33a-3p, miR-320a, and miR-93-5p showed the highest discriminating capacity for pancreatic neoplasia (PDAC or IPMN) with an area under the receiver operating characteristic curve (AUC) of 0.86, 0.85, 0.85, and 0.80, respectively. 2-miRNA combinations improved these performances reaching AUC = 0.90 for “miR-33a-3p+miR-320a. ” Addition of CA19.9 increased the diagnostic potential of miRNA signatures even further achieving an AUC of 0.95 (93% sensitivity and 85% specificity) for the combination of “miR-33a-3p+miR-320a+CA19.9. ” CONCLUSIONS: Novel signatures combining miRNAs and CA19.9 could be used as noninvasive biomarkers for early detection of PDAC.
Specific function and modulation of teleost monocytes/macrophages: polarization and phagocytosis

Macrophages exist in most tissues and play a variety of functions in vertebrates. Teleost fish species are found in most aquatic environments throughout the world and are quite diverse for a group of vertebrate animals. Due to whole genome duplication and environmental adaptation, teleost monocytes/macrophages possess a variety of different functions and modulations compared with those of mammals. A deeper understanding of teleost monocytes/macrophages in the immune system will not only help develop teleost-specific methods of disease prevention but will also help improve our understanding of the various immune mechanisms in mammals. In this review, we summarize the differences in polarization and phagocytosis of teleost and mammalian macrophages to improve our understanding of the various immune mechanisms in vertebrates.