Miracle-dz/UniFAR-FTUs huggingface dataset:
Prognostic and clinicopathological signi cance of YAP in Colorectal carcinoma: A systematic review and meta-analysis

Lu Dai Nanjing Medical University Second A liated Hospital Xiao Jin Nanjing Medical University Second A liated Hospital Jiayan Wang Nanjing Medical University Second A liated Hospital Yilan Ma Nanjing Medical University Second A liated Hospital Haihao Yan Nanjing Medical University Second A liated Hospital Ye Jin Nanjing Medical University Second A liated Hospital Xiaojuan Zhu Nanjing Medical University Second A liated Hospital Zheng Liu (  liuzheng117@njmu.edu.cn ) Nanjing Medical University Second A liated Hospital
Isolation and Characterization of Fusarium Species and Fumonisins Contamination in Maize from Lower Eastern and Rift Valley Regions of Kenya

Maize serves as a staple food in many Sub-Sahara African (SSA) Countries. It is mostly susceptible to mycotoxins including aflatoxin and fumonisin contamination. Fumonisins are produced by the Fusarium species, predominantly Fusarium verticillioides. Fumonisins’ health hazards are documented in many parts of the world. However, few studies exist on fumonisin contamination in maize locally. The presence of Fusarium species and the associated fumonisin contamination of maize grown in Rift Valley and Lower Eastern regions of Kenya were assessed. Maize samples were collected from randomly selected households in three Counties from each of the two regions. Isolation and characterization of Fusarium species was done using Daniel et al., (2011) protocol. Envrologix Quick Tox Kit was used to quantify fumonisin levels. Aspergillus species was the most prevalent fungi species isolated (50.3%) followed by Fusarium species (39.3%) with F. verticillioides accounting for 80.8% of all Fusarium spp. Of the 200 samples analyzed, 133 (65.5%) had fumonisin levels below the level of detection (< 0.1 ppm), 63 (31.5%) had fumonisin level of between 0.1 ppm4.0 ppm and 4 (2.0%) sample had fumonisin levels of more than 4.0 ppm. Lower Eastern Region had higher proportion of samples with detectable fumonisin levels compared to Rift Valley Region (55.4% vs 11.1%). In conclusion Fusarium verticillioides commonly associated with fumonisin contamination of maize was a common fungus isolated in the study regions. It also showed that some of the maize samples consumed by the respondents have fumonisin levels that are above the internationally accepted levels. These results suggest that people are likely to be exposed to fumonisins that has been associated with adverse health hazards.
S0884291419004023 1..9

Wurtzite materials in alloys of rock salt compounds Yanbing Han, Samantha L. Millican, Jun Liu, Sage R. Bauers, Sebastian Siol, Stephan Lany, Mowafak Al-Jassim, Charles B. Musgrave, Aaron M. Holder, Andriy Zakutayev Materials Science Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA; and School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, China Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA Materials Science Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA; and Materials Science Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA Address all correspondence to this author. e-mail: andriy.zakutayev@nrel.gov Current address: Empa—Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland. This paper has been selected as an Invited Feature Paper.
Analysis of storage and secretion of von Willebrand factor in blood outgrowth endothelial cells derived from von Willebrand disease patients

Einthoven Laboratory for Experimental Vascular Medicine, Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands. Laboratory of Experimental Cardiology, University Medical Centre Utrecht, Utrecht, The Netherlands. Department of Plasma Proteins, Sanquin Research, Amsterdam, The Netherlands. Department of Pharmaceutics, UIPS, Utrecht University, Utrecht, The Netherlands. Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands. Department of Molecular Cell Biology, Leiden University Medical Center, Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.
EXPRESSION OF YEAST DNA MISMATCH REPAIR PROTEINS AND DNA BINDING CHARACTERISTICS OF THE YEAST CONDENSIN SMC2/SMC4 HETERODIMER by Suchaiya J ariangprasert

This thesis describes initial experiments on two separate projects. The first project involved the expression and purification of the DNA mismatch repair proteins Mlh 1 and Pms 1. Expression vectors were made to singly and co-express these two proteins in yeast. Unfortunately, the proteins were found to be insoluble and this project was put on hold. The second project involved studying the DNA binding characteristics of the Smc2/4 core subunits of the yeast condensin complex. During these studies, it was found that the relatively low ionic strength buffer used for most published DNA binding assays caused these proteins to precipitate. In a higher salt buffer that maintained solubility, Smc2/4 heterodimer was found to bind preferentially to M13mp18 single strand DNA. These results are discussed in light of on-going experiments with the condensin complex.
Molecular Basis of H 2 O 2 Resistance Mediated by Streptococcal Dpr DEMONSTRATION OF THE FUNCTIONAL INVOLVEMENT OF THE PUTATIVE FERROXIDASE CENTER BY SITE-DIRECTED MUTAGENESIS IN STREPTOCOCCUS SUIS *

H 2 O 2 is an unavoidable cytotoxic by-product of aero- bic life. Dpr, a recently discovered member of the Dps protein family, provides a means for catalase-negative bacteria to tolerate H 2 O 2 . Potentially, Dpr could bind free intracellular iron and thus inhibit the Fenton chem-istry-catalyzed formation of toxic hydroxyl radicals (H 2 O 2 (cid:1) Fe 2 (cid:1) 3 (cid:1) OH (cid:1) – OH (cid:1) Fe 3 (cid:1) ). We explored the in vivo function of Dpr in the catalase- and NADH peroxi-dase-negative pig and human pathogen Streptococcus suis . We show that: (i) a Dpr allelic exchange knockout mutant was hypersensitive ( (cid:1) 10 6 -fold) to H 2 O 2 , (ii) Dpr incorporated iron in vivo , (iii) a putative ferroxidase center was present in Dpr, (iv) single amino acid substitutions D74A or E78A to the putative ferroxidase center abolished the in vivo iron incorporation, and (v) the H 2 O 2 hypersensitive phenotype was complemented by wild-type Dpr or by a membrane-permeating iron chelator, but not by the site-mutated forms of Dpr. These results demonstrate that the putative ferroxidase center of Dpr is
Title : Ultra scale-down approaches to study the centrifugal harvest for viral vaccine production . † Short running title : Centrifugation ultra scale-down for viral vaccines

Title: Ultra scale-down approaches to study the centrifugal harvest for viral vaccine production. † Short running title: Centrifugation ultra scale-down for viral vaccines Author List: Beatrice J Melinek, Sandrine Dessoy*, Bernice Wright, Dan G Bracewell, Tarit K Mukhopadhyay Affiliations: + Dept. of Biochemical Engineering, Bernard Katz building, University College London, Gordon Street, London, WC1H 0AH * GSK Vaccines, Rixensart, Belgium Email for correspondence: ucbetkm@ucl.ac. uk Author Telephone numbers: Beatrice J Melinek: 07984330623 Dan Bracewell: +44(0)2076799580 Tarit Mukhopadhyay: Extn. 30438
Converted P-to-S phase and Moho quality beneath the New Madrid seismic Zone from receiver function studies

Receiver functions from numerous teleseismic earthquakes recorded at 26 broadband seismic stations within the New Madrid Seismic Zone (NMSZ) were analyzed to map crustal thickness, Poisson’s ratio and Moho thickness within and around the NMSZ. The results show that the average crustal thickness of the stations within the Mississippi embayment is 33.4±0.28 km. The crust thins to about 25.5±1.09 km at OXF and thickens to about 43.3±0.12 km at PLAL. In the Mazatazal Belt, the crust varies from 30.2±0.14 to 53.1±0.29 km thick. However, the crust is thicker at WCI (53.1±0.29 km). There is a dramatic change in crustal thickness between the cratonic stations and stations within the Mississippi embayment. Our results from the Mazatzal belt are comparable to other Proterozoic and Archean shields that include reasonably well determined Moho depths, mostly based on receiver functions. The average crustal thickness for all shields is 39 km, while the average for Proterozoic shields is 40 km, and the average for Achaean shields is 38 km. We observed a dramatic change in crustal thickness from stations with the Mississippi embayment over a very short distance.
OPTH_A_229879 257..267

Laura Ceriotti Silvia Balzaretti Salvatore Barone Marisa Meloni 1 1VitroScreen, Milan, Italy; 2NTC S.r. l. , Milan, Italy Objective: To assess the eye tolerability of a buffered ophthalmic solution containing microglycine (sodium hydroxymethylglycinate, mwater) in an in vitro model. Materials and Methods: A multiple endpoint analysis (MEA) approach was applied to the reconstructed human corneal epithelium (HCE) model. Sodium hydroxymethylglycinate solution (0.04%) and two ophthalmic ointments containing microglycine (Protectorial, containing 0.02% of sodium hydroxymethylglycinate, and Edenight, containing 0.04% of sodium hydroxymethylglycinate) were investigated. The buffered solution and the ointments were tested on HCE after acute (one application in 24 hrs, followed or not by 16 hrs of recovery) or repeated (one application per day for three consecutive days) exposures; benzalkonium chloride (BAK) 0.01% and saline isotonic solution were used as positive and negative controls, respectively. Cellular viability, trans-epithelial electrical resistance (TEER), lactate dehydrogenase (LDH) release and histo-morphology were evaluated. Results: BAK 0.01% toxicity in HCE was confirmed for the 24+16 hrs acute and repeated exposure protocols, while, after 24–hours acute treatment, only modifications of the superficial cell layer were visible compared with the negative control. Sodium hydroxymethylglycinate had a very good tolerability profile and a neutral impact on the corneal surface after acute or repeated exposure. The Protectorial and Edenight ointments preserved cell viability in the different exposure protocols, suggesting a good local tolerability profile. Modifications of the superficial layers were observed on histo-morphological analysis and confirmed by increased release of LDH after 24+16 hrs acute exposure (+65% and +76% for Protectorial and Edenight, respectively) and TEER values after 24+16 hrs and 72 hrs exposure protocols. These results were dependent on the ointments’ accumulation on the corneal epithelium due to their physical form (semi-solid) and lipophilic properties. Conclusion: Sodium hydroxymethylglycinate, alone or as part of eye ointments, was found to be non-toxic after acute or repeated exposure in the reconstructed HCE model.
Antimicrobial and antioxidant activities of endophytes from Citrus Limon

Citrus limon is native to Asia and are used routinely by people for various purpose to make medicine. It also used to treat scurvy as it is rich in vitamin C. The antibacterial activity, antioxidant activity and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples which included leaves, stems and roots were used. 24 morphotypes were isolated from the 97 segements 27 root samples, 42 leaf samples and 28 stem samples). Fusarium sp showed the greatest antibacterial activity against Staphylococcus aureus and antioxidant activity. Twelve species showed proteolytic activity, particularly Fusarium sp. Ten species showed positive for lipase activity and three species showed positive in cellulase and all the isolates were showed negative in amylase activity. It is clear that the endophytic fungi from Citrus limon have potential for the production of bioactive compounds and may be a sources of new therapeutic agents for the effective treatment of diseases in humans. To our knowledge, this is the first study of endophytic fungi from different tissues of C.limon and their biotechnological potential.
Myopic Traction Maculopathy: Diagnostic and Management Strategies

: Pathologic myopia (PM) is an ocular disorder characterized by a spherical equivalent (SE) of more than – 6.0 diopters (D) or by an axial length (AL) of more than 26.5 millimeters (mm). PM is associated with myopic maculopathy (MM). The ATN classification describes all the aspects of MM which regroups atrophic, tractional and neovascular consequences to the sclera, choroid and retina of highly myopic eyes. The advent of OCT allowed to define the ultrastructural characteristics of the tractional changes in MM, described by the term myopic traction maculopathy (MTM). They include foveoschi-sis/maculoschisis/retinoschisis (FS/MS/RS), retinal/foveal detachment (RD/FD), lamellar macular holes (LMH) and full-thickness macular holes (FTMH) with or without RD (MHRD). The MTM staging system (MSS) describes all foveal and retinal changes related to MTM and their natural history interpreting them as different stages of a single progressive disorder. The management of MTM can be just observation for the earliest cases with good vision or surgery for the severe stages with vision loss. There are two possible surgical approaches: ab externo, that acts on the alteration of the scleral shape and includes posterior scleral reinforcement and macular buckle. Ab interno, that targets the alteration of the foveal profile and consists in pars plana vitrectomy with removal of all the epiretinal tractions, maneuvers on the internal limiting membrane, and the use of intravitreal tamponade and laser. As they target two different sides of the same pathology, the two techniques have to be selected on the base of the MTM stage, single or combined.
Photothermal Enhanced Photocatalytic Properties of Titanium Dioxide (B)/Glass Fiber Cloth

Titanium dioxide (B)/glass fiber cloth (B-T/GFC) composites with photothermal enhanced photocatalytic properties were prepared by using a facile chemical synthesis and Sol-Gel method, using the glass fiber cloth and TiH2 as substrate and precursor, respectively. XRD, SEM and TEM were employed to investigate the composition, structure and morphology of B-T/GFC composite. Different properties of the materials were studied by spectrophotometer, optical contact angle measuring instrument and solar simulator. The results show that the composite surface is composed of anatase black TiO2 with a disordercoating of about 1 nm. Meanwhile, the B-T/GFC composite with high hydrophobicity has strong absorption ability in the wavelength range from 250 nm to 2000 nm. Compared with glass fiber cloth, the B-T/GFC composite reveals better photothermal ability under sunlight with one solar intensity (1 kW/m). The photocatalytic degradation ability of B-T/GFC composite in organic pollutants cutting waste solution is better than those of pure black TiO2 (B-T) and P25. After irradiation for 2 h, the degradation of COD over B-T/GFC composite is about 1.3 times higher than that of P25. The B-T/GFC composite possessing excellent photothermal enhanced photocatalytic properties performance may lead to a range of novel applications.
Impact of a Drought on the Social Structure of a Lion Population in the Amboseli Ecosystem, Kenya

This study looked at the impact of severe drought in 2009 on lion population density and social structure in Amboseli National Park. The covered a period of six years, three years before (2007-2009) and three years after the drought (2010-2012). We used Rainfall Variability, Drought Severity Index, Prey Abundance Index and human-induced mortality to assess lion vulnerability to the extreme drought. The Vulnerability Index (VI) indicates response of lion populations to drought in terms of social structure, diet and mortality. The VI was negative in 2010, the year directly following the drought. This negative VI coincided with a changes in lion social structure (sex ratio, adult: juvenile ratio and group size) and increased mortality. The VI was positive in 2011, this was the time the lion population showed signs of recovery, with high reproduction. We witnessed 28 lions being killed (15 in 2010 alone) around the park during the post-drought period 2010-2012, compared to 14 before and during the drought (2007-2009), among them five pride males. After the drought, the lion population showed a significant change in structure, with a decrease in male: female sex ratio (due to a decline of males), a significant increase in adult group size and a significant decline in juvenile to adult ratio (due to increase of juveniles). All these changes correspond to increased lion vulnerability in 2010. We noticed that during this period, male lions were targeted more than females by Maasai warriors (Moran), probably due to cultural practice (use of manes and claws). The increase of juveniles was probably a result of less competition between pride males and consequently less infanticide. While the reduced group size was probably a response to lower prey densities and a drought-re-
Accountability and good governance are essential to deliver health services

Bulletin of the World Health Organization | August 2006, 84 (8) Abstract In the fields of health and development, donors channel multiple resources into the design of new practices and technologies, as well as small-scale programmes to test them. But successful practices are rarely scaled up to the level where they beneficially impact large, impoverished populations. An effective process for change is to use the experiences of new practices gained at the programme level for full-scale implementation. To make an impact, new practices need to be applied, and supported by management systems, at many organizational levels. At every level, potential implementers and likely beneficiaries must first recognize some characteristics that would benefit them in the new practices. An effective change process, led by a dedicated internal change agent, comprises several well-defined phases that successively broaden and institutionalize the use of new practices.
RECEIVED POULTRY LITTER APPLICATION

Land application of poultry litter to pasture elevates the concentration of phosphorus in surface runoff, and it is becoming an increasing problem in sensitive water bodies. The objectives of this study were to assess the effects of soil test phosphorus (STP), surface application of poultry litter, rainfall/runoff sequences, and time after litter application on dissolved reactive phosphorus (DRP) in surface runoff from pasture in a greenhouse experiment using rainfall simulation. Treatment factors were poultry litter at a rate of 0.0 and 6.7 Mg ha‐1, low and high STP, and three rainfall/runoff sequences (RRS). The latter refers to runoff‐producing rainfall events starting from day 1, day 4, and day 7 after litter application. The study also included a rainfall simulation study of pasture field plots to investigate the effects of poultry litter and time after application on DRP in surface runoff, which was used to corroborate the greenhouse study. In both studies, runoff samples were taken at the end of 30 min of continuous runoff. Treatment effects on DRP concentrations in surface runoff were analyzed using ANOVA procedures using an alpha = 0.05. For the greenhouse study, poultry litter application, RRS, and time after litter application were found to have a highly significant effect on DRP concentration in surface runoff. Poultry litter had a significant effect on DRP concentrations in surface runoff until 18 days after litter application compared to the controls. Between 18 and 32 days after litter application, the effect on DRP became insignificant for any level of STP or rainfall sequence. A rainfall event without runoff reduced DRP concentration in the first surface runoff events by more than 50%. For the field plot study, DRP decreased rapidly with time, thereby corroborating the greenhouse study. The effect of poultry litter on DRP became statistically insignificant sometime between 35 and 161 days after application.
IJOPM-10-2020-0698_proof 100..126

Purpose – In recent years, many companies have decided to decertify from their previously adopted corporate social responsibility (CSR) standards. The aim of this paper is to explore the phenomenon by focusing on the most important auditable CSR standard: Social Accountability 8000 (SA8000). Design/methodology/approach – First, an event study is performed on a dataset composed of 136 SA8000 decertified public listed companies to analyse the possible relationship between certification, decertification and firms’ operating performance. Second, the authors shed light on the differences between 94 SA8000 (still) certified and the abovementioned 136 decertified firms. Finally, 10 interviews are conducted with decertified firms in the dataset to deepen the outcomes of the previous analyses. Findings –The results show that, despite an initial positive effect in terms of sales and profitability, decertified companies experienced a reduction in productivity and profitability in the years following the certification, while positive outcomes emerged after the decertification. The study also highlights that certified and decertified firms differ in terms of home country, industry and labour intensity. Originality/value – The paper contributes to the literature by opening the debate on an important but unexplored research area: the decertification from the most popular CSR standard, i.e. SA8000, and its relationship with firms’ performance. In doing this, it also highlights the main differences between decertified and certified companies.
Circulation Topic Review Most Read in Circulation: Cardiovascular Quality and Outcomes

The following articles are being highlighted as part of Circulation’s Topic Review series. This series will summarize the most important manuscripts, as selected by the editors, published in Circulation and the Circulation subspecialty journals. The studies included in this article represent the most read manuscripts published on the topic of cardiovascular quality and outcomes in 2009 and 2010. (Circulation. 2011;124:e544-e551.)
Role of Nitric Oxide , Muscarinic Receptors , and the ATP-Sensitive K ' Channel in Mediating the Effects of Acetylcholine to Mimic Preconditioning in Dogs

The aims of the current study were to examine the efflicacy of acetylcholine (ACh) to mimic ischemic preconditioning in dogs and to determine the role of nitric oxide (NO), muscarinic receptors, and ATP-sensitive K' (KATP) channels in mediating its effects. Barbital-anesthetized open-chest dogs were subjected to 60 minutes of left anterior descending coronary artery (LAD) occlusion followed by 4 hours of reperfusion. Preconditioning was elicited by 10 minutes of LAD occlusion followed by 10 minutes of reperfusion before the 60-minute occlusion period. ACh (3 or 10 gg/min) or an equivalent volume of saline was infused into the LAD for 10 minutes, followed by a 10-minute drug-free period before the 60-minute ischemic insult. In other groups, the specific NO synthesis inhibitor NG_monomethyl-Larginine (L-NMMA, 4 mg/min), the muscarinic receptor antagonist-NO synthesis inhibitor nitro-Larginine methyl ester (L-NAME, 3 mg/min), or the specific KATP channel blocker 5-hydroxydecanoate (5-HD, 3 mg/min) was infused with ACh into the LAD for 10 minutes. The infusion of L-NAME, L-NMMA, or 5-HD was started 2 minutes before ACh infusion. Transmural myocardial blood flow was measured at 5 minutes of occlusion, and infarct size was determined by triphenyltetrazolium staining and expressed as a percentage of the area at risk. There were no significant differences in collateral blood flow