Miracle-dz/UniFAR-FTUs huggingface dataset:
Repurposing Dimetridazole and Ribavirin to disarm Pseudomonas aeruginosa virulence by targeting the quorum sensing system

Pseudomonas aeruginosa relies on its complex cellular regulatory network to produce a series of virulence factors and to cause various acute and chronic infections in a wide range of hosts. Compared with traditional antibiotics which frequently accompany with widespread antibiotic resistance, crippling the virulence system of bacteria is expected to be a promising anti-infective strategy. In this study, Dimetridazole and Ribavirin, which had poor antibacterial activities on P. aeruginosa reference isolate PAO1 in nutrient medium but significantly inhibited the growth of P. aeruginosa PAO1 in M9-adenosine, were selected from 40 marketed compounds with similar core structure (furan, benzofuran, or flavonoids) to the acyl-homoserine lactone signals of P. aeruginosa quorum sensing (QS) system. The production of QS-controlled proteases, pyocyanin, and biofilm formation of P. aeruginosa PAO1 and the clinical isolates were significantly decreased by the presence of Dimetridazole or Ribavirin. Correspondingly, the majority of QS-activated genes in P. aeruginosa, including the key regulatory genes lasR, rhlR, and pqsR and their downstream genes, were significantly inhibited by Ribavirin or Dimetridazole, as determined by RNA-sequencing and quantitative PCR. Furthermore, the susceptibilities of drug-resistant P. aeruginosa isolates to polymyxin B, meropenem, and kanamycin were remarkably promoted by the synergistic application of Dimetridazole or Ribavirin. Finally, the treatment of Ribavirin or Dimetridazole effectively protected Caenorhabditis elegans and mice from P. aeruginosa infection. In conclusion, this study reports the antivirulence potentials of Dimetridazole and Ribavirin on P. aeruginosa and provides structural basis and methodological reference for the development of anti-pseudomonal drugs.
Community characteristics of autotrophic CO2-fixing bacteria in karst wetland groundwaters with different nitrogen levels

Karst wetlands are important in the global carbon and nitrogen cycles as well as in security of water resources. Huixian wetland (Guilin) is the largest natural karst wetland in China. In recent years, groundwater nitrogen pollution has increasingly affected the wetland ecosystem integrity due to anthropogenic activities. In this study, it was hypothesized that autotrophic microbial diversity is impacted with the advent of pollution, adversely affecting autotrophs in the carbon and nitrogen cycles. Autotrophic microbes have important roles in abating groundwater nitrogen pollution. Thus, it is of great significance to study the characteristics of autotrophic bacterial communities and their responses to environmental parameters in nitrogen-polluted karst groundwaters. The abundances of the Calvin–Benson cycle functional genes cbbL and cbbM as well as the autotrophic CO2-fixing bacterial communities were characterized in the karst groundwater samples with different levels of nitrogen pollution. The cbbM gene was generally more abundant than the cbbL gene in the groundwater samples. The cbbL gene abundance was significantly positively correlated with dissolved inorganic nitrogen (DIN) concentration (P < 0.01). In the autotrophic CO2-fixing bacterial communities, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria of the phylum Proteobacteria were predominant. At the genus level, Rubrivivax and Methylibium were the dominant cbbL gene containing genera, while Halothiobacillus and Endothiovibrio were the dominant genera for the cbbM gene. The abundance of autotrophic CO2-fixing bacterial communities increased but their diversity decreased with the inflow of nitrogen into the karst groundwater system. The community structure of autotrophic CO2-fixing bacteria in the groundwaters was also significantly affected by environmental factors such as the carbonic anhydrase (CA) activity, dissolved inorganic carbon (DIC) concentration, temperature, and oxidation-reduction potential (ORP). Nitrogen inflow significantly changed the characteristics of autotrophic CO2-fixing bacterial communities in the karst groundwaters. Some key genera such as Nitrosospira and Thiobacillus were clearly abundant in the karst groundwaters with high nitrogen levels. Their respective roles in nitrification and denitrification impact nitrogen removal in this ecosystem. The findings in this study provide an important reference for biological abatement of nitrogen pollution in the karst groundwater system.
nNOS-derived NO modulates force production and iNO-derived NO the excitability in C2C12-derived 3D tissue engineering skeletal muscle via different NO signaling pathways

Nitric oxide (NO) is a bioactive gas produced by one of the three NO synthases: neuronal NOS (nNOS), inducible (iNOS), and endothelial NOS (eNOS). NO has a relevant modulatory role in muscle contraction; this takes place through two major signaling pathways: (i) activation of soluble guanylate cyclase and, thus, protein kinase G or (ii) nitrosylation of sulfur groups of cysteine. Although it has been suggested that nNOS-derived NO is the responsible isoform in muscle contraction, the roles of eNOS and iNOS and their signaling pathways have not yet been clarified. To elucidate the action of each pathway, we optimized the generation of myooids, an engineered skeletal muscle tissue based on the C2C12 cell line. In comparison with diaphragm strips from wild-type mice, 180 myooids were analyzed, which expressed all relevant excitation–contraction coupling proteins and both nNOS and iNOS isoforms. Along with the biochemical results, myooids treated with NO donor (SNAP) and unspecific NOS blocker (L-NAME) revealed a comparable NO modulatory effect on force production as was observed in the diaphragm strips. Under the effects of pharmacological tools, we analyzed the myooids in response to electrical stimulation of two possible signaling pathways and NO sources. The nNOS-derived NO exerted its negative effect on force production via the sGG-PKG pathway, while iNOS-derived NO increased the excitability in response to sub-threshold electrical stimulation. These results strengthen the hypotheses of previous reports on the mechanism of action of NO during force production, showed a novel function of iNOS-derived NO, and establish the myooid as a novel and robust alternative model for pathophysiological skeletal muscle research.
Performance comparison of hybrid architectures for millimeter wave communicatins

The expected massive proliferation of wireless systems points out an exponential increase in the number of users until 2020, which is needed to support up to one thousand times more traffic and connections in order of Gigabit per second. However, these goals require a significantly improvement in the spectral and energy efficiency. As a result, it is essential to make a paradigm shift in conventional wireless systems, imposed by the introduction of fifth generation (5G). For this purpose, new and promising transmission techniques will be needed, namely the use of millimeter Waves (mmWave) in systems with a massive number of antenna elements. Nevertheless, considerable challenges emerge in the adoption of these techniques. On one hand, mmWave suffer great difficulties in terms of propagation. On the other hand, the using of conventional architectures for systems with a large number of antennas is absolutely impracticable because of the costs and the level of complexity. This happens because the signal processing in physical layer is mostly done in baseband, which means, that one RF chain for each antenna is required. In this context the hybrid architectures are a relatively recent proposal where the aim is to simplify the use of a large number of antenna elements, dividing the processing between the analog and digital domains. Moreover, the number of RF chains needed are much lower than the total number of antenna elements of the system, which contribute to obvious improvements in terms of complexity, costs and energy consumption. In this Dissertation a hybrid mmWave based architecture, where each RF chain is only connected to a small set of antennas, is implemented. It is considered a system comprising a transmitter and a receiver both equipped with a massive number of antennas and where the number of RF chains is much lower than the number of antennas. Hybrid analog/digital precoders recently proposed in the literature are used and a new hybrid analog/digital equalizer is designed. The implemented architecture is then evaluated and compared with other architecture, where all the antennas are connected to all RF chains.
Case report: Cytokine hemoadsorption in a case of hemophagocytic lymphohistiocytosis secondary to extranodal NK/T-cell lymphoma

We discuss a single case of Hemophagocytic lymphohistiocytosis (HLH) due to NK-type non-Hodgkin lymphoma and Epstein-Barr virus reactivation with multiorgan dysfunction and distributive shock in which we performed cytokine hemoadsorption with Cytosorb ®. A full microbiological panel was carried out, including screening for imported disease, standard serologies and cultures for bacterial and fungal infection. A liver biopsy and bone marrow aspirate were performed, confirming the diagnosis. The patients fulfilled the HLH-2004 diagnostic criteria, and according to the 2018 Consensus Statements by the HLH Steering Committee of the Histiocyte Society, dexamethasone and etoposide were started. There was an associated hypercytokinemia and, due to refractory distributive shock, rescue therapy with cytokine hemoadsorption was performed during 24 h (within day 2 and 3 from ICU admission). After starting this procedure, rapid hemodynamic control was achieved with a significant reduction in vasopressor support requirements. This case report highlights that cytokine hemoadsorption can be an effective since rapid decrease in IL-10 levels and a significant hemodynamic improvement was achieved.
Editorial: Mechanisms guarding the genome

Department of Biological Sciences, University of Limerick, Limerick, Ireland, Limerick Digital Cancer Research Centre, HRI, ULCaN, University of Limerick, Limerick, Ireland, Lambe Institute for Translational Research, Discipline of Pathology, Centre for Chromosome Biology, National University of Ireland, Galway, Ireland, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, United States. , Cancer and Ageing Research Program, School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Translational Research Institute, Queensland University of Technology, Brisbane, QLD, Australia
Foreign direct investment entry mode and China’s carbon productivity based on spatial econometric model

As the world’s largest developing country and the largest carbon emitter, China must consider economic growth and carbon emission reduction in development. Therefore, improving carbon productivity is an important goal of China at present. At the same time, China’s foreign capital inflow has always been at the forefront of the world, and foreign direct investment (FDI) has had various impacts on China’s carbon productivity. Based on the panel data of 25 provinces in China from 2007 to 2019, this paper uses a spatial econometric model to study the difference in the impact of FDI on China’s carbon productivity under different entry modes. The study found that: when FDI enters China in the mode of joint ventures, there is a positive spatial spillover effect, which is conducive to improving China’s carbon productivity; while when FDI enters China in the mode of wholly foreign-owned enterprises, there is a negative spatial spillover effect, which will inhibit the improvement of China’s carbon productivity. Therefore, when introducing foreign capital, the Chinese government should formulate differentiated foreign investment policies according to the different entry modes of FDI, and encourage more FDI to enter China in the mode of joint ventures.
A method for identifying moonlighting proteins based on linear discriminant analysis and bagging-SVM

Moonlighting proteins have at least two independent functions and are widely found in animals, plants and microorganisms. Moonlighting proteins play important roles in signal transduction, cell growth and movement, tumor inhibition, DNA synthesis and repair, and metabolism of biological macromolecules. Moonlighting proteins are difficult to find through biological experiments, so many researchers identify moonlighting proteins through bioinformatics methods, but their accuracies are relatively low. Therefore, we propose a new method. In this study, we select SVMProt-188D as the feature input, and apply a model combining linear discriminant analysis and basic classifiers in machine learning to study moonlighting proteins, and perform bagging ensemble on the best-performing support vector machine. They are identified accurately and efficiently. The model achieves an accuracy of 93.26% and an F-sorce of 0.946 on the MPFit dataset, which is better than the existing MEL-MP model. Meanwhile, it also achieves good results on the other two moonlighting protein datasets.
Advances in Takayasu arteritis: An Asia Pacific perspective

Takayasu Arteritis (TA) is a rare form of chronic granulomatous large vessel vasculitis that is more common in Asia compared to other parts of the world. There have been several developments in the field of Takayasu arteritis in relation to genetics, classification, clinical features, imaging, disease activity assessment and management and much of these works have been done in the Asia Pacific region. We will be discussing selected few in the current review.
Does the gender of your co-worker matter? Evidence from call centres in India

men on their fertility decisions, their children’s education and health, their spouse’s labour force participation and autonomy etc. still remain under-studied. This paper finds some evidence for the impact of gender diversity at the workplace on the productivity of men with regressive gender attitudes. I find that men with regressive gender attitudes show up to work for the same proportion of days as men with regressive gender attitudes but have lower daily productivity. This finding suggests that there can be potential gains in productivity with investments in programs to improve gender attitudes among young men.
EFF Comments on TCG Design, Implementation and Usage Principles 0.95

The Electronic Frontier Foundation (EFF) is pleased to submit these comments on the Trusted Computing Group (TCG)’s ”Design, Implementation, and Usage Principles” draft version 0.95. TCG, together with its predecessor the Trusted Computing Platform Alliance (TCPA), has been the subject of intense criticism in the on-line community over projected applications of its trusted platform module (TPM). Most of these applications are speculative; some are possible within the TCG specifications, and others are not. Many depend on what platform or operating system vendors do. Some abusive applications might be subject to market discipline, while others could be tolerated by many consumers or be largely invisible most of the time. In some cases, consumers might have little recourse because of limited and imperfect competition.
Chapter 12 Business-Cycle Transmission Mechanism in ASEAN+3: Financial Integration or Trade?

The recent global recession requires policymakers to identify the relative importance of shock-transmission mechanisms in each region and devise policy counter-measures against future external-country shocks. In the past decade world dynamics have changed considerably due to increased openness and integration, requiring examination of the characteristics of business cycles at regional levels. This chapter shows that due to the presence of a short-term causal relationship of financial variables with GDP in the Association of South-East Asian Nations Plus Three (ASEAN+3) region, the slowdown contagion spread to most countries within the region. The slowdown triggered a trade variables shock-transmission mechanism, too. As a result, we observe co movements of business cycle of the region with the business cycle of the shock originating external country. Therefore, business cycle convergence and decoupling phenomena of the region to the shock originating external country (US) depend not only on the origin of the shock in the external country but also on the relative importance of the transmission mechanisms between shock originating external countries and other regions. For policy purposes, knowing the correct transmission mechanism will help in tailoring an appropriate response to the idiosyncratic disturbance and is helpful in achieving long-term regional development goals.
Transport properties of CoGe1.5Se1.5

We have synthesized and characterized polycrystalline n- and p-type CoGe 1 . 5 Se 1 . 5 , a member of the class of compounds with the skutterudite crystal structure. Both specimens have large room temperature Seebeck coefficients and resistivities. The carrier type depends very sensitively on the atomic ratio between Ge and Se. For both specimens, the carrier mobilities are very low and the thermal conductivity is lower than that of the binary skutterudite CoSb 3 . Some of these properties can be attributed to the vacancies in the crystal lattice due to the nonstoichiometric nature of the specimens studied. The transport properties are compared with those of CoSb 3 , and the potential for thermoelectric applications is discussed. This work is part of a continuing effort to explore the large phase space of many possible compositions of skutterudites, a promising class of high-efficiency thermoelectric materials.
Low-frequency noise properties in Pt-indium gallium zinc oxide Schottky diodes

The low-frequency noise properties of Pt-indium gallium zinc oxide (IGZO) Schottky diodes at different forward biases are investigated. The IGZO layer and Pt contact were deposited by RF sputtering at room temperature. The diode showed an ideality factor of 1.2 and a barrier height of 0.94 eV. The current noise spectral density exhibited 1/f behavior at low frequencies. The analysis of the current dependency of the noise spectral density revealed that for the as-deposited diode, the noise followed Luo's mobility and diffusivity fluctuation model in the thermionic-emission-limited region and Hooge's empirical theory in the series-resistance-limited region. A low Hooge's constant of 1.4 × 10−9 was found in the space-charge region. In the series-resistance-limited region, the Hooge's constant was 2.2 × 10−5. After annealing, the diode showed degradation in the electrical performance. The interface-trap-induced noise dominated the noise spectrum. By using the random walk model, the interface-trap density was .
Risk Factors and Mechanism of Hemorrhagic Transformation of Acute Ischemic Cerebral Infarction

A feared complication of ischemic cerebrovascular accident (CVA) is hemorrhagic transformation and it increases the length of hospital admissions, results in poor clinical outcomes and increases mortality. This study aims to identify risk factors of hemorrhagic transformation, discuss the pathogenesis of transformation and prognosis of the condition from original studies conducted within the past 10 years. PubMed and EMBASE are the major databases used. Eight articles met the inclusion criteria and are a mixture of retrospective and prospective studies. The sample size ranges from 32 to 1484 patients. Generally, the mechanism of hemorrhagic transformation identified involves the release of reactive oxygen species and metalloproteinase as a result of cerebral ischemia. Metalloproteinase further causes a breakdown of the extracellular matrix, thereby giving rise to the extravasation of cellular components. Some identified risk factors include: age, use of antithrombotic, the subtype of ischemic stroke, the severity of stroke and hypertension. Prognosis is linked to the type of hemorrhagic infarctions according to the European Cooperative Acute Stroke Study II (ECASS II) with patients with either hemorrhagic infarct type 2 (HI2) and parenchymal hematoma type 2 (PH2) having the worst functional outcome.
The Maximum Degree of an Exponentially Distributed Random Graph

Let G G (n, p) be a graph on n vertices where each pair of vertices is joined independently with probability p for 0 < p < 1 and q = 1 p. In this work, we introduce weighted random graf G with exponential distribution and investigate that the probability that every vertex of G has degree at most np + b√pqn is equal to 0.595656764.
THE EFFECT OF SERVICE QUALITY ON COMMUNITY SATISFACTION IN TLOGOSARI DISTRICT

The purpose of this study was to examine and analyze the effects of partially and simultaneouslythe dimensions of service quality consisting of reliability, responsiveness, assurance, empathyand physical evidence on community satisfaction in Tlogosari District, Bondowoso Regency.This study uses a survey research form with a questionnaire as a data collection tool which isdistributed to 75 (respondents) communities in Tlogosari District by selecting samples byaccidental sampling. Testing is carried out using validity and reliability tests. Data processingwas analyzed using descriptive statistics with multiple linear regression analysis techniquesusing SPSS 22.0. Based on the results of the study, it was concluded that there was a significantinfluence between reliability, responsiveness of empathy and physical evidence on thesatisfaction of the people of Tlogosari District, while guarantee had no significant effect on thesatisfaction of the people of Tlogosari District. Simultaneously all dimensions of service qualityhave a significant effect on community satisfaction in Tlogosari District.Keywords: service quality, community satisfaction, subdistrict
The Unexpected Hanging And Other Mathematical Diversions

A magnetic recording element suitable for use in an automatic ticket vending unit and an automatic ticket checking and collecting unit which comprises a substrate and a layer coated thereon, said coating layer comprising 100 parts, by weight, of gamma -ferrite particles, 1 to 2 parts of a dispersing agent for gamma -ferrite, 5 to 15 parts of an alkaline modified protein, 15 to 30 parts of a rubber-like synthetic polymer and 1 to 2 parts of a hardening agent for protein is disclosed.