Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficacy of Tocilizumab in COVID-19: Single-Center Experience

Background Cytokine release syndrome can be observed during the course of COVID-19. Tocilizumab is used for treating this highly fatal syndrome. We think that the starting time of tocilizumab is important. In this article, we aimed to discuss the efficacy of tocilizumab and to review the necessity of starting it in the early period and the laboratory values that guide us in determining the time of this early period. Methods This retrospective study includes a total of 308 patients with a diagnosis of COVID-19 who were treated with tocilizumab, who were hospitalized in the University of Health Sciences, Gazi Yaşargil Training and Research Hospital between July 2020 and December 2020. The data of the patients were recorded on the day of hospitalization, the day of taking tocilizumab (day 0), and the 1st day, 3rd day, 7th day, and 14th day after taking tocilizumab. Data included age, gender, underlying diseases, where the patient was followed, duration of symptoms before admission to the hospital, duration of oxygen demand before tocilizumab, fever, saturation, and laboratory values. Patients were divided into the mortality group (group 1) and the survival group (group 2), and all data were compared. Results The study consisted of 308 COVID-19 patients divided into two groups: the mortality group (group 1, n = 135) and the survival group (group 2, n = 173). The median age of the patients was 60 (min–max: 50-70) years, 75.3% (n = 232) were male, and 56.8% had at least one comorbidity. While 88.9% of group 1 was in the intensive care unit, 26.6% of group 2 received tocilizumab while in the intensive care unit, and there was a statistically significant difference. Median SpO2 values and lymphocyte counts were significantly lower in group 1 than in group 2, both on the day of hospitalization and on the day of the first dose of tocilizumab treatment (p < 0.001 for both). C-reactive protein, d-dimer, and alanine aminotransferase values were higher in the mortal group on the first day of hospitalization, and this was significant (p = 0.021, p = 0.001, and p = 0.036, respectively). In our study, d-dimer was 766.5 ng/mL in the survivor group and 988.5 ng/mL in the mortal group. In our patient group, the mean lymphocyte count was 700 × 103/mm3 in the group that survived the first day of TCZ and 500 × 103/mm3 in the mortal group. In addition, the CRP value was 135.5 mg/L in the survivor group and 169 mg/L in the mortal group. There was no difference between ferritin values. Conclusions Tocilizumab is still among the COVID-19 treatment options and appears to be effective. But the start time is important. In order to increase its effectiveness, it may be important to know a cut-off value of the laboratory findings required for the diagnosis of cytokine release syndrome. Further studies are needed for this.
Perceptions of professional soccer coaches, support staff and players toward virtual reality and the factors that modify their intention to use it

A small evidence base supports the use of virtual reality in professional soccer, yet there is a lack of information available on perceptions and desire to use the technology from those employed at professional soccer clubs. Therefore, the aim of the study was to compare and quantify the perceptions of virtual reality use in soccer, and to model behavioural intentions to use this technology. This study surveyed the perceptions of coaches, support staff, and players in relation to their knowledge, expectations, influences and barriers of using virtual reality via an internet-based questionnaire. To model behavioural intention, modified questions and constructs from the Unified Theory of Acceptance and Use of Technology were used, and the model was analysed through partial least squares structural equation modelling. Respondents represented coaches and support staff (n = 134) and players (n = 64). All respondents generally agreed that virtual reality should be used to improve tactical awareness and cognition, with its use primarily in performance analysis and rehabilitation settings. Generally, coaches and support staff agreed that monetary cost, coach buy-in and limited evidence base were barriers towards its use. In a sub-sample of coaches and support staff without access to virtual reality (n = 123), performance expectancy was the strongest construct in explaining behavioural intention to use virtual reality, followed by facilitating conditions (i.e., barriers) construct which had a negative association with behavioural intention. Virtual reality has the potential to be a valuable technology within professional soccer although several barriers exist that may prevent its widespread use.
eTosis and pyroptosis (7). Pyroptosis leads to myocardial perforation and rupture of the cell membrane and releases pro‐inflammatory factors such as interleukin‐1β (il‐1β) and IL‐18, initiated by caspase‐1, causing a local inflammatory response (8). Previous studies

interferon regulatory factor (irF) 2 is a transcrip‐ tion factor belonging to the irF family, which is essential for gasdermin d (GSdMd)‐induced pyroptosis. decreasing myocardial cell pyroptosis confers protection against heart damage and cardiac dysfunction caused by myocardial infarc‐ tion (Mi). The aim of the present study was to investigate the involvement of irF2 in Mi and the underlying mechanism of irF2 in pyroptosis. To mimic Mi, ligation of the left ante‐ rior descending coronary artery was performed to establish an in vivo mouse model and rat cardiomyocytes H9c2 cells were cultured under hypoxic conditions to establish an in vitro model. Transthoracic echocardiography was used to assess cardiac function. Hematoxylin and eosin staining was used to observe histopathological changes in the myocardial tissue. immunohistochemistry and western blotting were performed to detect irF2 expression levels. Tunel staining and flow cytometry were used to detect apoptosis in myocar‐ dial tissue and cells. chromatin immunoprecipitation and dual luciferase reporter assay were used to verify the effect of irF2 on GSdMd transcription. irF2 was upregulated in Mi mice. Mi induced pyroptosis, as evidenced by increased GSdMd, n‐terminal GSdMd (GSdMd‐n), and cleaved (c‐) caspase‐1 levels. Mi increased il‐1β and il‐18 levels. These alterations were alleviated by irF2 silencing. Furthermore, in hypoxia‐treated H9c2 cells, IRF2 silencing significantly decreased the elevated levels of il‐1β and il‐18 and pyrop‐ tosis‐associated proteins, including GSdMd, GSdMd‐n and c‐caspase1. Moreover, in hypoxia‐treated H9c2 cells, irF2 directly bound to the GSdMd promoter to drive GSdMd transcription and promote pyroptosis and irF2 expression may be regulated via the hypoxia inducible factor 1 signaling pathway. in conclusion, the present results demonstrated that irF2 is a key regulator of Mi by mediating pyroptosis, which triggers GSdMd activation.
Institutional mechanisms for local government accountability: Evaluating the Nigerian provisions against the European charter of local self-government

This study examines the role of institutional mechanisms in local government accountability by undertaking a comparative assessment of the Nigerian institutional provisions against the European Charter of Local Self-Government. The specific objectives of the study are to examine the institutional mechanisms for local government accountability in Nigeria, evaluate the Nigerian mechanisms, using the provisions of the European Charter of Local Self-Government and subsequently determine how the effective deployment of institutional mechanisms may enhance the prospects of local government accountability. The study adopts as methodology a comparative analytical framework, under which it uses the provisions of the European Charter of Local Self-Government to evaluate the Nigerian institutional mechanisms. Findings of the study led to a conclusion that for emerging political systems, which Nigeria typifies, institutionalized mechanisms of (political) accountability in the local government system, are truly less effective than the non-institutionalized or informal mechanisms. For the effective deployment of institutional mechanisms to enhance the prospects of local government accountability, the study concludes that a judiciary component of the local structures of governance is an unconditional necessity, as in the absence of such conditions; the incidence at the local level, totally ceases to be describable as government.
MODELING TERRAIN ANALYSIS EXPERTISE TO SUPPORT COMMAND AND CONTROL IN THE FIELD

Prepared through collaborative participation in the Advanced Decision Architectures Consortium sponsored by the U. S. Army Research Laboratory under the Collaborative Technology Alliance Program, Cooperative Agreement DAAD19-01-2-0011. The U. S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation thereon.
On the Extraction of Purely Motor EEG Neural Correlates during an Upper Limb Visuomotor Task.

Deciphering and analyzing the neural correlates of different movements from the same limb using electroencephalography (EEG) would represent a notable breakthrough in the field of sensorimotor neurophysiology. Functional movements involve concurrent posture co-ordination and head and eye movements, which create electrical activity that affects EEG recordings. In this paper, we revisit the identification of brain signatures of different reaching movements using EEG and present, test, and validate a protocol to separate the effect of head and eye movements from a reaching task-related visuomotor brain activity. Ten healthy participants performed reaching movements under two different conditions: avoiding head and eye movements and moving with no constrains. Reaching movements can be identified from EEG with unconstrained eye and head movement, whereas the discriminability of the signals drops to chance level otherwise. These results show that neural patterns associated with different arm movements could only be extracted from EEG if the eye and head movements occurred concurrently with the task, polluting the recordings. Although these findings do not imply that brain correlates of reaching directions cannot be identified from EEG, they show the consequences that ignoring these events can have in any EEG study that includes a visuomotor task.
Cancer immunotherapy using artificial adjuvant vector cells to deliver NY‐ESO‐1 antigen to dendritic cells in situ

NY‐ESO‐1 is a cancer/testis antigen expressed in various cancer types. However, the induction of NY‐ESO‐1‐specific CTLs through vaccines is somewhat difficult. Thus, we developed a new type of artificial adjuvant vector cell (aAVC‐NY‐ESO‐1) expressing a CD1d‐NKT cell ligand complex and a tumor‐associated antigen, NY‐ESO‐1. First, we determined the activation of invariant natural killer T (iNKT) and natural killer (NK) cell responses by aAVC‐NY‐ESO‐1. We then showed that the NY‐ESO‐1‐specific CTL response was successfully elicited through aAVC‐NY‐ESO‐1 therapy. After injection of aAVC‐NY‐ESO‐1, we found that dendritic cells (DCs) in situ expressed high levels of costimulatory molecules and produced interleukn‐12 (IL‐12), indicating that DCs undergo maturation in vivo. Furthermore, the NY‐ESO‐1 antigen from aAVC‐NY‐ESO‐1 was delivered to the DCs in vivo, and it was presented on MHC class I molecules. The cross‐presentation of the NY‐ESO‐1 antigen was absent in conventional DC‐deficient mice, suggesting a host DC‐mediated CTL response. Thus, this strategy helps generate sufficient CD8+ NY‐ESO‐1‐specific CTLs along with iNKT and NK cell activation, resulting in a strong antitumor effect. Furthermore, we established a human DC‐transferred NOD/Shi‐scid/IL‐2γcnull immunodeficient mouse model and showed that the NY‐ESO‐1 antigen from aAVC‐NY‐ESO‐1 was cross‐presented to antigen‐specific CTLs through human DCs. Taken together, these data suggest that aAVC‐NY‐ESO‐1 has potential for harnessing innate and adaptive immunity against NY‐ESO‐1‐expressing malignancies.
1H detection and dynamic nuclear polarization–enhanced NMR of Aβ1-42 fibrils

Significance Amyloid-β (Aβ) is the subject of intense scrutiny because of its close association with Alzheimer’s disease (AD), which currently afflicts about 50 million people worldwide. The results reported in this manuscript focus on the new possibilities provided by ultrafast magic-angle spinning (MAS) 1H detection and fast-MAS dynamic nuclear polarization (DNP), which have ushered in a new era for NMR-based structural biology, but whose potential has not yet been fully exploited for the structural investigation of complex amyloid assemblies. This work demonstrates the expeditious structural analysis of amyloid fibrils, without requiring preparation of large sample amounts, and sets the stage for future studies of unlabeled AD peptides derived from tissue samples available in limited quantities. Several publications describing high-resolution structures of amyloid-β (Aβ) and other fibrils have demonstrated that magic-angle spinning (MAS) NMR spectroscopy is an ideal tool for studying amyloids at atomic resolution. Nonetheless, MAS NMR suffers from low sensitivity, requiring relatively large amounts of samples and extensive signal acquisition periods, which in turn limits the questions that can be addressed by atomic-level spectroscopic studies. Here, we show that these drawbacks are removed by utilizing two relatively recent additions to the repertoire of MAS NMR experiments—namely, 1H detection and dynamic nuclear polarization (DNP). We show resolved and sensitive two-dimensional (2D) and three-dimensional (3D) correlations obtained on 13C,15N-enriched, and fully protonated samples of M0Aβ1-42 fibrils by high-field 1H-detected NMR at 23.4 T and 18.8 T, and 13C-detected DNP MAS NMR at 18.8 T. These spectra enable nearly complete resonance assignment of the core of M0Aβ1-42 (K16-A42) using submilligram sample quantities, as well as the detection of numerous unambiguous internuclear proximities defining both the structure of the core and the arrangement of the different monomers. An estimate of the sensitivity of the two approaches indicates that the DNP experiments are currently ∼6.5 times more sensitive than 1H detection. These results suggest that 1H detection and DNP may be the spectroscopic approaches of choice for future studies of Aβ and other amyloid systems.
Association between genetic variant rs2267716 of CRHR2 gene with colorectal cancer

Colorectal cancer (CRC) is the third most common cancer and one of the main causes of death around the world. Multiple lines of evidence have suggested the role of the corticotropin-releasing hormone (CRH) family in CRC induction, including the low expression of corticotropin-releasing hormone receptor 2 (CRHR2), which is an angiogenesis inhibitor and inflammatory modulator. Previous research suggests that CRHR2 expression in colonic intestinal cells can regulate migration, proliferation and apoptosis through the modulation of several pathways. The aim of this study was to analyze the association of the rs10250835, rs2267716 and rs2267717 variants of CRHR2 gene with CRC in the Mexican population in order to consider its predictive value in CRC. This cross-sectional study included a group of 187 unrelated patients with sporadic CRC and a control group of 191 healthy blood donors. DNA extraction from peripheral blood was carried out using the Miller method. Identification of the rs10250835 variant was performed using PCR-restriction fragment length polymorphism (RFLP) and the rs2267716 and rs2267717 variants using TaqMan allelic discrimination assay. The minor allele homozygous CC of the rs2267716 variant of CRHR2 showed significant difference between CRC and control group (p=0.025), as well as the GCA haplotype (p=0.007), corresponding to the rs10250835, rs2267716 and rs2267717 variants, respectively. Our results suggest that the rs2267716 variant and GCA haplotype of CRHR2 represent a risk factor for CRC development in Mexican patients.
Positivity and Boundedness of Solutions for a Stochastic Seasonal Epidemiological Model for Respiratory Syncytial Virus (RSV)

En este trabajo se investiga la positividad y acotamineto de la solucion de un modelo epidemiologico estacional estocastico para el virus respiratorio sincitial (RSV ). La estocasticidad en el modelo se debe a entornos fisicos y sociales fluctuantes y se introduce perturbando el parametro de transmision de la enfermedad. Se demuestra la existencia y unicidad de la solucion positiva del modelo epidemiologico estacional estocastico, lo cual se requiere en el modelado de las poblaciones ya que todas las poblaciones deben ser positivos desde el punto de vista biologico. Adicionalmente, la positividad y la acotacion de las soluciones es importante para otros modelos no lineales que se presentan en las ciencias y la ingenieria. Las simulaciones numericas del modelo estocastico se realizan utilizando el esquema numerico de Milstein y se incluyen para apoyar los resultados analiticos.
ADAR1 RNA editing regulates endothelial cell functions via the MDA-5 RNA sensing signaling pathway

Cellular RNA transcripts in endothelial cells, especially the SINE RNAs, need extensive editing by the A-to-I RNA editing enzyme ADAR1, which is essential for neonatal survival by regulating the MDA-5–mediated RNA-sensing signaling pathway. The RNA-sensing signaling pathway has been well studied as an essential antiviral mechanism of innate immunity. However, its role in non-infected cells is yet to be thoroughly characterized. Here, we demonstrated that the RNA sensing signaling pathway also reacts to the endogenous cellular RNAs in endothelial cells (ECs), and this reaction is regulated by the RNA-editing enzyme ADAR1. Cellular RNA sequencing analysis showed that EC RNAs endure extensive RNA editing, especially in the RNA transcripts of short interspersed nuclear elements. The EC-specific deletion of ADAR1 dramatically reduced the editing level on short interspersed nuclear element RNAs, resulting in newborn death in mice with damage evident in multiple organs. Genome-wide gene expression analysis revealed a prominent innate immune activation with a dramatically elevated expression of interferon-stimulated genes. However, blocking the RNA sensing signaling pathway by deletion of the cellular RNA receptor MDA-5 prevented interferon-stimulated gene expression and rescued the newborn mice from death. This evidence demonstrated that the RNA-editing/RNA-sensing signaling pathway dramatically modulates EC function, representing a novel molecular mechanism for the regulation of EC functions.
Designing Combinations of Pictogram and Text Size for Icons: Effects of Text Size, Pictogram Size, and Familiarity on Older Adults’ Visual Search Performance

Objective This study aimed to determine suitable combinations of text and pictogram sizes for older adults and investigated the visual prioritization of pictogram versus text. Background Icons have become an indispensable part of application (app) design. Pictogram size and text size of icons influence the usability of apps, especially by aged users. However, few studies have investigated the influences of different pictogram and text size combinations on readability, legibility, and visual search performance for older adults. Method This study used eye-tracking technology to investigate the effects of different pictogram and text size combinations as well as familiarity on readability, legibility, and visual search performance for older adults. A 3 (pictogram size) × 3 (text size) × 2 (familiarity) repeated-measures experimental design was used. Results The results of this study suggest that pictogram size and text size significantly affect visual search performance and that familiarity moderates the effect of text size on distribution of fixation duration proportion for text and pictograms. Conclusion Large pictogram and text sizes improved the readability and legibility of icons for older adults. Furthermore, the older adults fixated the area of text prior to pictograms when the pictogram size was larger than 72 × 72 px (1.38° × 1.38°) in the visual search task. Application The results of this study suggest using different combinations of pictogram and text sizes for older adults under different scenarios. The findings of this study act as practical support for designers and developers of mobile apps for older adults.
Motion Generation for Biped Robots Based on Minimal Energy Control

Biped robot pattern generators based on parametrized-time trajectories are usually criticized ba sed on their poor performance in handling unexpected pertur- bations. The main problem is caused by the mismatch be- tween the desired and the actual state of the robot at the time of foot impact. In this paper, based on the state space representation of the humanoid robot's mass concentrated model, we propose a pattern generator that computes the ZMP input that transfers the CoM in one step from an initial desired state to a final desired state expending the minimum amount of energy. Constraints on the input to avoid foot ro- tation on supporting conditions are easily handled due to its hyperbolic characteristics. We show on simulation the effectiveness of the method and its similarity to human mo- tion generation.
Multipartite Quantum States and their Marginals

Subsystems of composite quantum systems are described by reduced density matrices, or quantum marginals. Important physical properties often do not depend on the whole wave function but rather only on the marginals. Not every collection of reduced density matrices can arise as the marginals of a quantum state. Instead, there are profound compatibility conditions -- such as Pauli's exclusion principle or the monogamy of quantum entanglement -- which fundamentally influence the physics of many-body quantum systems and the structure of quantum information. The aim of this thesis is a systematic and rigorous study of the general relation between multipartite quantum states, i.e., states of quantum systems that are composed of several subsystems, and their marginals. In the first part, we focus on the one-body marginals of multipartite quantum states; in the second part, we study general quantum marginals from the perspective of entropy.
A counterexample to Stein’s Equi-$n$-square Conjecture

In 1975 Stein conjectured that in every $n\times n$ array filled with the numbers $1, \dots, n$ with every number occuring exactly $n$ times, there is a partial transversal of size $n-1$. In this note we show that this conjecture is false by constructing such arrays without partial transverals of size $n-\frac{1}{42}\ln n$.