Indian in Homoeopathy Comparative study of High-performance thin-layer Comparative study of High-performance thin-layer chromatography and Antioxidant potential of Hydrocotyle asiatica chromatography and Antioxidant potential of Hydrocotyle asiatica mother tincture used in Homoeopathy mother tinctur

Abstract BackgroundBackground: Hydrocotyle asiatica has a therapeutic significance in the Indian system of medicine due to its rich antioxidant activity. In Homoeopathy, Hydrocotyle asiatica is used for the treatment of jaundice, skin diseases, dropsy, elephantiasis, leprosy, gonorrhea, leucorrhea, and nervous debility. It contains the abundant triterpene glycoside Asiatic acid which shows cytotoxic activity on cancer cells. Its homoeopathic mother tincture is a major source of antioxidant compounds, which is responsible for its overall pharmacological activity. ObjectivesObjectives: This study was done to evaluate antioxidant activity and High-Performance Thin-Layer Chromatography study of Hydrocotyle asiatica in-house homoeopathic mother tincture and market samples. Materials and Methods: Materials and Methods: Antioxidant activity of in-house homoeopathic mother tincture (A) and three market samples (B, C, and D) were determined by 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free-radical scavenging activity, total phenol, and 2,2’-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay methods. High-performance thin-layer chromatography (HPTLC) study performed on precoated silica gel 60 F254 TLC plate, mobile phase used was toluene: ethyl acetate: formic acid (5.5:4.5:1, v/v/v) and UV detection were performed at 254 and 366 nm. For derivatization, an anisaldehyde sulfuric acid reagent was used. ResultsResults: The tincture of Hydrocotyle asiatica prominent antioxidant activity. Abstract ( treatment of related chronic Background: Hydrocotyle asiatica has a therapeutic significance in the Indian system of medicine due to its rich antioxidant activity. In Homoeopathy, Hydrocotyle asiatica is used for the treatment of jaundice, skin diseases, dropsy, elephantiasis, leprosy, gonorrhea, leucorrhea, and nervous debility. It contains the abundant triterpene glycoside Asiatic acid which shows cytotoxic activity on cancer cells. Its homoeopathic mother tincture is a major source of antioxidant compounds, which is responsible for its overall pharmacological activity. Objectives: This study was done to evaluate antioxidant activity and High-Performance Thin-Layer Chromatography study of Hydrocotyle asiatica in-house homoeopathic mother tincture and market samples. Materials and Methods: Antioxidant activity of in-house homoeopathic mother tincture (A) and three market samples (B, C, and D) were determined by 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free-radical scavenging activity, total phenol, and 2,2’-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay methods. High-performance thin-layer chromatography (HPTLC) study performed on precoated silica gel 60 F 254 TLC plate, mobile phase used was toluene: ethyl acetate: formic acid (5.5:4.5:1, v/v/v) and UV detection were performed at 254 and 366 nm. For derivatization, an anisaldehyde sulfuric acid reagent was used. Results: The homoeopathic mother tincture of Hydrocotyle asiatica had prominent antioxidant activity. HPTLC study indicated the presence of triterpene glycoside compound Asiatic acid in chloroform extract of Hydrocotyle asiatica . Conclusion: The mother tinctures prepared by authenticated plant samples showed maximum active constituents and prominent antioxidant activity as compared to the mother tinctures procured from the market. The present study justifies the homoeopathic usage of Hydrocotyle asiatica and highlights its healing properties. bestimmt. Die HPTLC-Studie wurde auf einer vorbeschichteten Kieselgel 60 F254 TLC-Platte durchgeführt, als mobile Phase wurde Toluol:Ethylacetat:Ameisensäure (5,5:4,5:1, v/v/v) verwendet und die UV-Detektion erfolgte bei 254 und 366 nm. Für die Derivatisierung wurde ein Anisaldehyd-Schwefelsäure-Reagenz verwendet. Ergebnisse: Das Ergebnis zeigte, dass der antioxidative Test der homöopathischen Urtinktur von Hydrocotyle asiatica eine herausragende antioxidative Aktivität aufwies. Die HPTLC-Studie zeigte das Vorhandensein der Triterpenglykosidverbindung Asiatische Säure im Chloroformextrakt von Hydrocotyle asiatica. Schlussfolgerung: Die aus authentifizierten Pflanzenproben hergestellten Urtinkturen wiesen im Vergleich zu den aus dem Handel bezogenen Urtinkturen ein Maximum an aktiven Bestandteilen und eine ausgeprägte antioxidative Aktivität auf. Die vorliegende Studie rechtfertigt die homöopathische Verwendung von Hydrocotyle asiatica und unterstreicht seine heilenden Eigenschaften. realizado con HPTLC en placa de TLC de gel de sílice 60 F254 prerecubierta, la fase móvil utilizada fue tolueno:acetato de etilo:ácido fórmico (5,5:4,5:1, v/v/v) y detección UV a 254 y 366 nm. Para la derivación se utilizó un reactivo de ácido sulfúrico anisaldehído. Resultados: El resultado reveló que el análisis antioxidante de la tintura madre homeopática de Hydrocotyle asiatica tenía una actividad antioxidante prominente. El estudio de HPTLC indicó la presencia del compuesto glucósido triterpeno ácido asiático en el extracto de cloroformo de Hydrocotyle asiatica . Conclusión: Las tinturas madre preparadas por muestras vegetales autenticadas mostraron constituyentes activos máximos y actividad antioxidante prominente en comparación con las tinturas madre obtenidas del mercado. El presente estudio justifica el uso homeopático de Hydrocotyle asiatica y destaca sus propiedades curativas.