Miracle-dz/UniFAR-FTUs huggingface dataset:
COMPUTER SYSTEM VALIDATION: A REVIEW

Pharmaceutical industry regulatory compliance of computerized systems is of ever increasing importance. The regulations require specific record keeping on product safety, efficacy, strength, quality and purity. 21 CFR Part 210 and 211 and others apply to Pharmaceutical Products and 21 CFR Part 820 applies to Medical Devices. Life cycle include the stage of planning, specification, programing, testing, commissioning, documentation, operation, monitoring and modifying. The V-Model supports structured testing which is essential for successful validation. Qualification is the process of establishing appropriately documented verifications and tests that provide a high level of assurance that a computer system will operate in accordance with predefined specifications (DQ, IQ, OQ, PQ).
Construction of three‐gene‐based prognostic signature and analysis of immune cells infiltration in children and young adults with B‐acute lymphoblastic leukemia

Although B‐acute lymphoblastic leukemia (B‐ALL) patients' survival has been improved dramatically, some cases still relapse. This study aimed to explore the prognosis‐related novel differentially expressed genes (DEGs) for predicting the overall survival (OS) of children and young adults (CAYAs) with B‐ALL and analyze the immune‐related factors contributing to poor prognosis.
Outcomes in arthroscopic surgery and proposal rehabilitative treatment in femoral acetabular impingement syndrome

Background and aim of the work. Femoral Acetabular impingement syndrome (FAIS) is a pathologic condition that can lead to hip pain, functional limitation and stiffness. In the last few decades orthopedics and physiotherapists have improved both surgery and rehabilitative treatment leading to a better and better treatment. The target of this paper il to verify the efficiency of an early and multimodal physiotherapy treatment after and arthroscopic surgery of the FAIS. Materials and Methods. We performed arthroscopic treatment and rehabilitation on 19 patients with mean age of 37±8,3 years, 12 males and 7 females. Each patient has been evaluated preoperatively (T0), postoperatively after 6 week (T1) and after 3 months of follow up (T2), assessment was carried out by: administration of the VAS and WOMAC score for pain and function and joint examination of active hip movement through an inertial sensor system. Results. VAS score shows a decrease of pain after 6 week (mean decrease was 36%) and after 3 months (mean decrease was 33%). WOMAC score shows an increase of the functional performance of the hip after 3 weeks and after 3 months (in both phases the mean score increase of the 44%). At last, the analysis of the active movement and of the hip joint showed a generalized increase in all movements both 6 weeks and 3 months after surgery, in particular for flexion (with the knee flexed) and internal rotation movements. of the hip. Conclusion. The results of this study are in line with the current scientific literature and the protocol used represents a valid tool to complete the surgical treatment. The proposal of an early, intensive treatment combined with hydro-kinesitherapy seems to be safe and effective, however further studies are needed (increasing the sample size) to investigate the results.
Quadriceps or patellar ligament reconstruction with artificial ligament after total knee replacement

Background and aim: Ruptures of the quadriceps or patellar tendon after TKA implantation are a fearful and disabling complication. Direct reconstructions highlighted various problems, such as the decrease in extensor force, failure, need for prolonged post-operative immobilization. So augmentation techniques have been proposed with autologous tendons, allografts, artificial ligaments. Among these, the LARS seems the most modern and promising. For this reason, we have retrospectively reviewed a case series of patients operated with this artificial ligament, to assess the results and highlight the tips and tricks for this procedure. Methods: Ten patients with a mean age of 69.4 years suffered an extensor apparatus lesion after knee replacement. These patients underwent primary reconstruction and augmentation with LARS. They were retrospectively reviewed at a mean follow-up of 3.8 years, by measuring active flexion and extension, and by Lysholm scoring scale. Results: We did not observe any problems with healing of the surgical wound, nor phenomena of intolerance to the implanted material, such as inflammation, skin rashes or fistulas. The mean flexion was 117 degrees. Active extension was allowed in all patients, but with a mean extensor lag of 18 degrees. The mean Lysholm score was 74.2. Conclusions: LARS offers good results without completely solving the problem of extensor lag, linked to the softness of the tissues in the quadriceps. The major advantages of LARS were in the good tissue ingrowth, the absence of adverse tissue reactions, the ubiquitous availability and the possibility of stable fixation with early rehabilitation. (www.actabiomedica.it)
Investigation of the Involvement of HHV-6 Encoded Viral Chemokine Receptors in Autoimmune Thyroiditis Development

The study of human herpesvirus-6 (HHV-6) involvement in autoimmunity development is very challenging, due to the complex nature of this virus. HHV-6 is a ubiquitous, lifelong persistent, and immunomodulating virus, which mainly spreads in solid tissues using cell-to-cell mechanics, and thus can escape from the host’s immune response. ABSTRACT Human herpesvirus-6 (HHV-6) contains two genes (U12 and U51) that encode putative homologues of human G-protein-coupled receptors like CCR1, CCR3, and CCR5. It has been shown that these viral proteins can be expressed on the surface of epithelial and some peripheral blood mononuclear cells, suggesting that they could potentially induce autoimmunity. We aimed to investigate the possibility of HHV-6 encoded viral chemokine receptors (U12 and U51) involvement in autoimmune thyroiditis (AIT) development by detecting viral peptide specific antibodies in AIT patient samples. Seventy-nine AIT patients whose thyroid tissues were shown to be positive for HHV-6 and 32 blood donors were enrolled in this study. Twenty-eight synthetic peptides derived from HHV-6 U12 and U51 proteins’ amino acid sequences, as well as recombinant human CCR1, CCR3, and CCR5 proteins were used in suspension multiplex immunological assay to detect specific IgG and IgM antibodies. HHV-6 peptide specific IgG and IgM antibodies were found in patients’ samples. AIT patients' samples were found to be more frequently positive for peptide IgGs in comparison to control group’s samples. Even though peptide antibody cross-reactivity with human CCRs was not demonstrated, our results show a new immunogenic HHV-6 antigen—a possible new player in the HHV-6 induced autoimmunity exacerbation. IMPORTANCE The study of human herpesvirus-6 (HHV-6) involvement in autoimmunity development is very challenging, due to the complex nature of this virus. HHV-6 is a ubiquitous, lifelong persistent, and immunomodulating virus, which mainly spreads in solid tissues using cell-to-cell mechanics, and thus can escape from the host’s immune response. It has been implicated as an environmental factor in several autoimmune diseases. An association between HHV-6 and autoimmune thyroiditis has been demonstrated, yet clear mechanism of involvement remains to be elucidated, since the virus can be detected in nearly all autoimmune thyroiditis patient thyroid glands. Our results show new potentially immunogenic human herpesvirus-6 antigens—possible new players in the HHV-6 induced autoimmunity exacerbation, which could be subjects for further research. Together with previously published results, this study described possible mechanisms which may underlie the induction of autoimmune reactivities against thyroid tissues in AIT.
The pharmacological and pharmacokinetic properties of esculin: A comprehensive review

Cortex fraxini is a widely used traditional Chinese medicine. Esculin is one of the main active ingredients of Cortex fraxini and has attracted more and more attention from scholars. The purpose of the review is to systematically review relevant studies on the pharmacological effects and pharmacokinetic characteristics of esculin to support its further application as therapeutic agents. Pharmacological studies have shown that the anti‐inflammatory and anti‐oxidative stress effects of esculin are outstanding. This indicates that esculin is promising to be used to treat a variety of diseases closely related to inflammation and oxidative damage. Esculin has anti‐diabetic effect, which is closely related to improving pancreas damage, promoting insulin release, and enhancing glucose homeostasis. In addition, esculin has anti‐cancer, antibiosis, anti‐virus, neuroprotection, anti‐thrombosis and treating eye diseases properties. Pharmacokinetic studies show that esculin can be quickly and evenly distributed in the body. However, the first pass effect of esculin is serious. In short, esculin is promising to treat many diseases, but further high quality studies are needed to firmly establish the clinical efficacy of esculin.
Rural-Urban Differences in Cognition: Findings From the Advanced Cognitive Training for Independent and Vital Elderly Trial

Objectives We examined associations between three geographic areas (urban, suburban, rural) and cognition (memory, reasoning, processing speed) over a 10-year period. Methods: Data were obtained from 2539 participants in the Advanced Cognitive Training for Independent and Vital Elderly (ACTIVE) trial. Multilevel, mixed-effects linear regression was used to estimate cognitive trajectories by geographical areas over 10 years, after adjusting for social determinants of health. Results: Compared to urban and suburban participants, rural participants fared worse on all cognitive measures—memory (B = −1.17 (0.17)), reasoning (B = −1.55 (0.19)), and processing speed (B = 0.76 (0.19)) across the 10-year trajectory. Across geographic areas, greater economic stability, health care access and quality, and neighborhood resources were associated with better cognition over time. Discussion: Findings highlight the importance of geographical location when examining cognition later in life. More research examining place-based life experiences is needed to make the greatest impact on geographically diverse communities.
Microbiota manipulation through the secretion of effector proteins is fundamental to the wealth of lifestyles in the fungal kingdom

Abstract Fungi are well-known decomposers of organic matter that thrive in virtually any environment on Earth where they encounter wealths of other microbes. Some fungi evolved symbiotic lifestyles, including pathogens and mutualists, that have mostly been studied in binary interactions with their hosts. However, we now appreciate that such interactions are greatly influenced by the ecological context in which they take place. While establishing their symbioses, fungi not only interact with their hosts but also with the host-associated microbiota. Thus, they target the host and its associated microbiota as a single holobiont. Recent studies have shown that fungal pathogens manipulate the host microbiota by means of secreted effector proteins with selective antimicrobial activity to stimulate disease development. In this review, we discuss the ecological contexts in which such effector-mediated microbiota manipulation is relevant for the fungal lifestyle and argue that this is not only relevant for pathogens of plants and animals but also beneficial in virtually any niche where fungi occur. Moreover, we reason that effector-mediated microbiota manipulation likely evolved already in fungal ancestors that encountered microbial competition long before symbiosis with land plants and mammalian animals evolved. Thus, we claim that effector-mediated microbiota manipulation is fundamental to fungal biology.
Gait Planning of a Novel Metamorphic Quadruped Robot

： Mobile robots raised much interest in recent years due to their maneuverability in complicated environments. A novel quadruped robot which has a changeable waist and possess new gaits is introduced. Based on the kinematics model, the reconfigurable waist of the novel metamorphic quadruped robot is analyzed and the body configuration switching rules are proposed. The reachable area of robot leg is compared with a normal quadruped of a rigid waist. Taking waist action into consideration, two novel gaits, i.e. twisting forward gait and spinning gait, are designed and their processes are described. To demonstrate advantages of the metamorphic waist, stability margin of a rigid-body gait and that of the reconfigurable-body gait are compared, the minimum size of a narrow corner for the robot to walk through is calculated and the constraints to achieve the smooth motion of the visual point and center of gravity of body (COG) are analyzed.
Performance limits on joint estimation of frequency offset, channel and noise variance in aeronautical telemetry

Modern digital communication techniques leverage the capabilities of advanced digital signal processing to improve the bit error rate performance of modulated carrier in harsh radio frequency (RF) environments. This improvement requires the knowledge of the RF environment (e.g., frequency offset, multipath channel coefficients, noise variance). In this paper we derive the theoretical performance bound of the joint frequency offset, channel and noise variance estimators. Performance of the joint maximum likelihood estimators are compared with this bound for two of the measured aeronautical telemetry channels with iNET-formatted SOQPSK-TG samples. INTRODUCTION The air-to-ground wireless downlink channel in aeronautical telemetry suffers from multipath interference due to reception of radio signal via multiple propagation paths. Although narrow beamwidth of ground-based auto-tracking antennas can reduce the multipath interference by attenuating signal from paths other than line-of-sight, it can do so only when the elevation angle of the airborne transmitter is high. Delays observed on test ranges for low-elevation angle flight paths [1, 2] show that multipath interference is dominant when the elevation angle is lower. Moreover, complexity of air-borne test articles has grown significantly over the last several decades which demands higher data rates (10-20 Mbits/s) in modern aeronautical telemetry systems. Consequently, bandwidth of the modulated carrier has grown up to such an extent that essentially all the multipath fading are frequency selective. Due to this frequency selective nature multipath interference is the dominant cause of link outage in aeronautical telemetry. To alleviate multipath interference spatial diversity and/or equalization can be used. Spatial diversity requires multiple transmit and/or receive antennas and thus raises the system cost. In any
β Cell–specific deletion of Zfp148 improves nutrient-stimulated β cell Ca2+ responses

Insulin secretion from pancreatic β cells is essential for glucose homeostasis. An insufficient response to the demand for insulin results in diabetes. We previously showed that β cell–specific deletion of Zfp148 (β-Zfp148KO) improves glucose tolerance and insulin secretion in mice. Here, we performed Ca2+ imaging of islets from β‑Zfp148KO and control mice fed both a chow and a Western-style diet. β-Zfp148KO islets demonstrated improved sensitivity and sustained Ca2+ oscillations in response to elevated glucose levels. β-Zfp148KO islets also exhibited elevated sensitivity to amino acid–induced Ca2+ influx under low glucose conditions, suggesting enhanced mitochondrial phosphoenolpyruvate-dependent (PEP-dependent), ATP-sensitive K+ channel closure, independent of glycolysis. RNA-Seq and proteomics of β-Zfp148KO islets revealed altered levels of enzymes involved in amino acid metabolism (specifically, SLC3A2, SLC7A8, GLS, GLS2, PSPH, PHGDH, and PSAT1) and intermediary metabolism (namely, GOT1 and PCK2), consistent with altered PEP cycling. In agreement with this, β-Zfp148KO islets displayed enhanced insulin secretion in response to l-glutamine and activation of glutamate dehydrogenase. Understanding pathways controlled by ZFP148 may provide promising strategies for improving β cell function that are robust to the metabolic challenge imposed by a Western diet.
To study the structural, electronic and optical properties of predicted stable halide perovskites ABX3

Perovskites have gained great interest in recent times. High efficiency solar materials have been developed from this family. Here in this work, structure, optical and electronic properties of recently artificial intelligence predicted stables perovskites ABX3 are studied through DFT approach. The structural analysis is done through the generalized gradient approximation. For the band structure calculations generalized gradient as well as the modified Becke-Johnson potential are used to investigate electrical and optical properties. The density of states and electronic band structure calculations reveal that the predicted stable halides Perovskites ABX3 have ultra-high to low band gaps. In the absence of experimental results, the calculated results can be valuable for further calculations. In the optical properties of the compounds dielectric function, optical reflectivity, absorption coefficient, optical conductivity, extinction coefficient, refractive index is calculated in different ranges of energy. The maximum value of static dielectric function is observed for KNiI3 and RbIrBr3 are 3.5 and 3 respectively. The highest absorption peak among the all is observed at 26 eV for SrLiF3, while refractive index of that SrLiF3 gives peak at 24 eV then refractive index decreases below the unity as energy increases up to 27.5 eV. The suitable energy bandgaps of the new compounds in the ultraviolet to infrared region certainly appeal for their use in the diverse applications.
Towards the Effects of Alignment Edits on the Quality of Experience of 360 Videos

The optimization of viewers' quality of experience (QoE) in 360 videos faces two major roadblocks: inaccurate adaptive streaming and viewers missing the plot of a story. Alignment edit emerged as a promising mechanism to avoid both issues at once. Alignment edits act on the content, matching the users' viewport with a region of interest in the video content. As a consequence, viewers' attention is focused, reducing exploratory behavior and enabling the optimization of network resources; in addition, it allows for a precise selection of events to be shown to viewers, supporting viewers to follow the storyline. In this work, we investigate the effects of alignment edits on QoE by conducting two user studies. Specifically, we measured three QoE factors: presence, comfort, and overall QoE. We introduce a new alignment edit, named \textit{Fade-rotation}, based on a mechanism to reduce cybersickness in VR games. In the user studies, we tested four versions of fade-rotation and compared them with instant alignment. We observed from the results that gradual alignment achieves good levels of comfort for all contents and rotational speed tested, showing its validity. We observed a decrease in head motion after both alignment edits, with the gradual edit reaching a reduction in head speed of 8\% greater than that of instant alignment, confirming the usefulness of these edits for streaming video on-demand. Finally, parameters to implement \textit{Fade-rotation} are described.
PERCORSI DI DIDATTICA INCLUSIVA CON L'USO DELLE TIC: IL PROGETTO AESSEDI INCLUSIVE LEARNING PLANS USING ICT: THE AESSEDI PROJECT

Education is a prime context for preventing social exclusion and providing people with special needs with opportunities for active participation in all spheres of life. A major objective of the school system is to ensure the participation of all students in the processes of learning and skills acquisition. This paper presents a pilot study concerning the design and classroom testing of learning plans based on the use of multimedia technologies developed for full inclusion of people with special needs. The learning plans are the result of collaborative efforts involving both curricular and special education teachers, who were supported by a web-based environment called AESSEDI; this represented scaffolding by providing a series of indicators supporting reflection on inclusion issues.
A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications

Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis due to its ability to grow on a wide range of low-cost feedstocks, including the waste gas carbon dioxide, whilst also naturally producing large quantities of polyhydroxybutyrate (PHB) during nutrient-limited conditions. Understanding the complex metabolic behaviour of this bacterium is a prerequisite for the design of successful engineering strategies for optimising product yields. We present a genome-scale metabolic model (GSM) of C. necator H16 (denoted iCN1361), which is directly constructed from the BioCyc database to improve the readability and reusability of the model. After the initial automated construction, we have performed extensive curation and both theoretical and experimental validation. By carrying out a genome-wide essentiality screening using a Transposon-directed Insertion site Sequencing (TraDIS) approach, we showed that the model could predict gene knockout phenotypes with a high level of accuracy. Importantly, we indicate how experimental and computational predictions can be used to improve model structure and, thus, model accuracy as well as to evaluate potential false positives identified in the experiments. Finally, by integrating transcriptomics data with iCN1361 we create a condition-specific model, which, importantly, better reflects PHB production in C. necator H16. Observed changes in the omics data and in-silico-estimated alterations in fluxes were then used to predict the regulatory control of key cellular processes. The results presented demonstrate that iCN1361 is a valuable tool for unravelling the system-level metabolic behaviour of C. necator H16 and can provide useful insights for designing metabolic engineering strategies.
Learning to Reverse DNNs from AI Programs Automatically

With the privatization deployment of DNNs on edge devices, the security of on-device DNNs has raised significant concern. To quantify the model leakage risk of on-device DNNs automatically, we propose NNReverse, the first learning-based method which can reverse DNNs from AI programs without domain knowledge. NNReverse trains a representation model to represent the semantics of binary code for DNN layers. By searching the most similar function in our database, NNReverse infers the layer type of a given function's binary code. To represent assembly instructions semantics precisely, NNReverse proposes a more fine-grained embedding model to represent the textual and structural-semantic of assembly functions.