Miracle-dz/UniFAR-FTUs huggingface dataset:
Pivoting an In-Person Multiplatform Science Program to a Virtual Program during a Pandemic: Lessons Learned

Abstract:School closures because of natural phenomena, such as COVID-19, underscore long-standing gaps in access to science education in the United States of America, particularly for young students. When educators have to pivot to deliver virtual instruction, it is important to identify feasible remote learning strategies for science content across formal and informal learning environments. This article discusses two evaluation studies of a multiplatform science learning program was originally developed for an in-person, formal learning environment that was modified based on infrastructure, preparations, and resource availability to meet the needs of distance learning in formal and informal learning environments due to COVID-19.
An international scholastic network to generate LexA enhancer-trap lines for Drosophila

Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and eleven insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science.
A TGF-β-responsive enhancer regulates SRC expression and epithelial–mesenchymal transition-associated cell migration

The non-receptor tyrosine kinase SRC is overexpressed and/or hyperactivated in various human cancers, and facilitates cancer progression by promoting invasion and metastasis. However, the mechanisms underlying SRC upregulation are poorly understood. In this study, we demonstrated that transforming growth factor-β (TGF-β) induces SRC expression at the transcriptional level by activating a novel intragenic SRC enhancer. In the human breast epithelial cell line MCF10A, TGF-β1 stimulation upregulated the SRC1A promoter, resulting in increased SRC mRNA and protein levels. Chromatin immunoprecipitation (ChIP)-sequencing analysis revealed that SMAD complex is recruited to three enhancer regions ~15 kb upstream and downstream of the SRC promoter, and one of them is capable of activating the SRC promoter in response to TGF-β. In addition, JUN, which is a member of the activator protein (AP)-1 family, also localizes to the enhancer and regulates TGF-β-induced SRC expression. Furthermore, the total amount of active SRC also increased, coinciding with the TGF-β-induced SRC expression. In TGF-β-induced epithelial–mesenchymal transition (EMT), TGF-β-induced SRC upregulation plays a crucial role in EMT-associated cell migration by activating the SRC/focal adhesion kinase (FAK) circuit. Overall, these results suggest that TGF-β-induced SRC upregulation promotes cancer cell invasion and metastasis in a subset of human malignancies.
S1742170521000399jra 618..623

Existing studies have examined the demand elasticities for organic products only in select categories, and their results for consumers’ sensitivity to price changes are inconsistent. Evidence regarding the effects of price promotions on the demand for organic foods vs non-organic foods is scarce. This study aims to (1) examine the own-price elasticities of organic foods vs non-organic counterparts both with and without a promotion in a variety of product categories, and (2) investigate how the distinctive promotion effects between organic and non-organic counterparts depend on food category features. Using purchase data for 36 food categories from the 2015 Nielsen Consumer Panel, we find differential own-price elasticities for organic and non-organic foods, regardless of whether the product is purchased with a promotion. When the products are purchased with a promotion, we find stronger price promotion effects of organic virtues than non-organic virtues and weaker price promotion effects of organic vices than conventional vices. Price promotions of organic foods are more likely to induce health-conscious consumers to switch from conventional purchases to organic purchases in virtues.
Reliability of MUSE 2 and Tobii Pro Nano at capturing mobile application users' real-time cognitive workload changes

Introduction Despite the importance of cognitive workload in examining the usability of smartphone applications and the popularity of smartphone usage globally, cognitive workload as one attribute of usability tends to be overlooked in Human-Computer Interaction (HCI) studies. Moreover, limited studies that have examined the cognitive workload aspect often measured some summative workloads using subjective measures (e.g., questionnaires). A significant limitation of subjective measures is that they can only assess the overall, subject-perceived cognitive workload after the procedures/tasks have been completed. Such measurements do not reflect the real-time workload fluctuation during the procedures. The reliability of some devices on a smartphone setting has not been thoroughly evaluated. Methods This study used mixed methods to empirically study the reliability of an eye-tracking device (i.e., Tobii Pro Nano) and a low-cost electroencephalogram (EEG) device (i.e., MUSE 2) for detecting real-time cognitive workload changes during N-back tasks. Results Results suggest that the EEG measurements collected by MUSE 2 are not very useful as indicators of cognitive workload changes in our setting, eye movement measurements collected by Tobii Pro Nano with mobile testing accessory are useful for monitoring cognitive workload fluctuations and tracking down interface design issues in a smartphone setting, and more specifically, the maximum pupil diameter is the preeminent indicator of cognitive workload surges. Discussion In conclusion, the pupil diameter measure combined with other subjective ratings would provide a comprehensive user experience assessment of mobile applications. They can also be used to verify the successfulness of a user interface design solution in improving user experience.
A DFX-based iron nanochelator for cancer therapy

Iron as an essential element, is involved in various cellular functions and maintaining cell viability, cancer cell is more dependent on iron than normal cell due to its chief characteristic of hyper-proliferation. Despite that some of the iron chelators exhibited potent and broad antitumor activity, severe systemic toxicities have limited their clinical application. Polyaminoacids, as both drug-delivery platform and therapeutic agents, have attracted great interests owing to their different medical applications and biocompatibility. Herein, we have developed a novel iron nanochelator PL-DFX, which composed of deferasirox and hyperbranched polylysine. PL-DFX has higher cytotoxicity than DFX and this effect can be partially reversed by Fe2+ supplementation. PL-DFX also inhibited migration and invasion of cancer cells, interfere with iron metabolism, induce phase G1/S arrest and depolarize mitochondria membrane potential. Additionally, the anti-tumor potency of PL-DFX was also supported by organoids derived from clinical specimens. In this study, DFX-based iron nanochelator has provided a promising and prospective strategy for cancer therapy via iron metabolism disruption.
Where and how - new insight for brown algal forest restoration in the Adriatic

Several anthropogenic factors are responsible for the decline of Cystoseira sensu lato (hereafter Cystoseira) forests along Mediterranean coasts. Some Cystoseira species are already regionally extinct, and their decline has been widely recorded. Sustainable and efficient techniques for the restoration of Cystoseira are needed. In this context, the objectives of this study were i) to analyse the reproductive traits of three populations of Gongolaria barbata from three nearby donor sites in the northern Adriatic Sea, assessing the differences in their reproductive potential and reproductive success; and ii) to evaluate the outplanting success in terms of the effectiveness of G. barbata restoration, in relation to the different donor and receiving sites (Miramare MPA and in front of the Marine Biology Station Piran - MBSP) and implemented methods (ex situ and hybrid method combining a mesocosm cultivation and a suspended culture in the field). After 2 weeks of cultivation in mesocosms, half of the tiles with germlings were transported to the receiving sites and placed on suspended lantern nets (hybrid method), which were later (after 3 months) transferred to the seabed on concrete plates with protective cages. The remaining tiles were placed on the seabed on concrete plates with protective cages after a 4-week culture (ex situ method). At both sites, lantern nets and plates were randomly placed at 3 m depth. Thallus length was measured monthly in each treatment. Seedlings in suspended culture showed lower performance at the Miramare MPA, most likely due to the unfavourable environmental conditions. The satisfactory results obtained at MBSP demonstrate the efficiency of the hybrid method and confirm its potential to reduce the cost and time required for cultivation. Since unpredictable climatic events pose the greatest threat to restoration performance, these challenges must be considered when establishing new restoration practices. Moreover, herbivore regulation is extremely urgent and should be planned and implemented on a larger regional scale.
Fermentation quality, bacterial community, and predicted functional profiles in silage prepared with alfalfa, perennial ryegrass and their mixture in the karst region

There is little information regarding the dynamics of fermentation products and the bacterial community in silage prepared with alfalfa (MS), perennial ryegrass (LP), and their mixture in the karst region. In this study, we explored the effects of combining MS with LP in different ratios (100% MS, 70% MS + 30% LP, 50% MS + 50% LP, 30% MS + 70% LP and 100% LP; fresh matter basis) on silage chemical composition, fermentation quality, bacterial communities and predicted functions during the ensiling process. Each treatment was prepared in triplicate and stored at room temperature (22–25°C) for 7, 15, and 45 days. The dry matter (DM) and water-soluble carbohydrate content of the silages increased as the LP proportion in the mixed silage increased; at 45 days, the 70% MS + 30% LP, 50% MS + 50% LP and 30% MS + 70% LP silages contained higher (p < 0.05) CP content than the 100% MS and 100% LP silages. The 30% MS + 70% LP and 100% LP silages exhibited lower (p < 0.05) pH and higher (p < 0.05) LA content than the other silages; at 45 days, none of the silages contained PA or BA. As fermentation proceeded, the abundance of harmful (Enterobacteriaceae and Sphingomonas) and beneficial (Lentilactobacillus, Lactiplantibacillus, Secundilactobacillus, and Levilactobacillus) microorganisms decreased and increased, respectively, as the LP proportion in the mixed silage increased. The predicted functional distribution of microbial communities and metabolic pathways revealed that the 30% MS + 70% LP and 100% LP silages had a stronger capacity for fermentation and a weaker capacity for nitrate reduction than the other silages. Moreover, as the fermentation proceeded, the 30% MS + 70% LP and 100% LP treatments enhanced the functions of “Metabolism,” “Genetic information processing” and “Organismal systems” at level 1, the functions of “Amino acid metabolism” and “Nucleotide metabolism” at level 2, and the functions of “Metabolic pathways,” “Biosynthesis of secondary metabolites,” “Biosynthesis of antibiotics” and “Purine metabolism” at level 3. Thus, adding LP could improve the fermentation quality of MS silage by changing the composition and metabolic function of microbes; furthermore, ensiling 30% alfalfa with 70% ryegrass can produce high-quality silage in the karst region.
Behavioral differences of individuals with different self-regulation levels in a real-life example of teamwork—DOTA 2

Teamwork is a vital aspect of human life, including a set of concrete behaviors which could be divided into various categories such as task performance, job dedication, backing up behavior, and monitoring. As an essential psychological factor could form team members to adapt to environmental changes, self-regulation has a marked impact on teamwork results. However, why self-regulation could affect results of teamwork in real life and how self-regulation influence the concrete teamwork behaviors remains unclear. This study recorded and extracted participants’ detailed gaming behaviors in Defense of the Ancients 2 (DOTA 2), which is an example of real-life teamwork scenario. The sample consisted of 59 DOTA 2 players with relative low-level self-regulation (93.22% male) and 59 with relative high-level self-regulation (96.61% male). Controlling confounding factors, we explored behavioral differences between the two groups in different types of heroes. Results showed that self-regulation influenced specific gaming behaviors including the categories of task performance, job dedication, and backing up behavior, but not including monitoring. Additionally, these impacts of self-regulation varied by hero type. These results demonstrate the different impacts of self-regulation on different categories of teamwork behaviors, and these impacts are considerably determined by individual’s role in the team. These findings shed light on the mechanism of the teamwork performance improvement caused by self-regulation and provide new insights into understanding the different impact patterns of self-regulation in different real-life tasks and responsibilities.
Editorial: Dynamic functional connectivity in neuropsychiatric disorders: Methods and applications, volume II

COPYRIGHT © 2022 Fu, Liu, Cui, Cheng, Liu and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Editorial: Dynamic functional connectivity in neuropsychiatric disorders: Methods and applications, volume II
Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence

Lumpy skin disease is caused by lumpy skin disease virus (LSDV), which can induce cattle with high fever and extensive nodules on the mucosa or the scarfskin, seriously influencing the cattle industry development and international import and export trade. Since 2013, the disease has spread rapidly and widely throughout the Russia and Asia. In the past few decades, progress has been made in the study of LSDV. It is mainly transmitted by blood-sucking insects, and various modes of transmission with distinct seasonality. Figuring out how the virus spreads will help eradicate LSDV at its source. In the event of an outbreak, selecting the most effective vaccine to block and eliminate the threat posed by LSDV in a timely manner is the main choice for farmers and authorities. At present, a variety of vaccines for LSDV have been developed. The available vaccine products vary in quality, protection rate, safety and side effects. Early detection of LSDV can help reduce the cost of disease. In addition, because LSDV has a huge genome, it is currently also used as a vaccine carrier, forming a new complex with other viral genes through homologous recombination. The vaccine prepared based on this can have a certain preventive effect on many kinds of diseases. Clinical detection of disease including nucleic acid and antigen level. Each method varies in convenience, accuracy, cost, time and complexity of equipment. This article reviews our current understanding of the mode of transmission of LSDV and advances in vaccine types and detection methods, providing a background for further research into various aspects of LSDV in the future.
Temporal video segmentation using a colour-texture histogram

We propose a new histogram generation method from the HSV colour space and show how it can be effectively used for temporal video segmentation. In our approach, combined human visual perception of colour and texture is captured in a single feature vector. Relative distribution of grey levels represents texture information while that of hue values represents colour information. This method shows high recall and precision of shot boundary detection in content-based video retrieval applications.
Visible-light-driven nanoscale zero-valent iron loaded rGO/g-C3N4 for fluoroquinolone antibiotics degradation in water

Fluoroquinolone antibiotics attract increasing attention in the water treatment field because of the potential adverse effects on aquatic ecosystems and human health. The graphitic carbon nitride (g-C3N4) based photocatalysis has been demonstrated as an economically feasible and environmentally benign process to control these persistent contaminants. In this study, a new visible-light-driven of reduced graphene oxide (rGO) and nanoscale zero-valent iron (nZVI) co-modified g-C3N4-based photocatalyst was synthesized via ultrasonication-assisted chemisorption method. The optimized nZVI-loaded rGO/g-C3N4 (10% IGCN) showed a reaction rate enhancement of 2.12∼3.69-fold and 1.20∼1.68-fold for the degradation of ofloxacin (OFL), norfloxacin (NOR), and ciprofloxacin (CIP) compared to that of carbon-doped g-C3N4 (MCB0.07) and rGO-supported g-C3N4 (7.5% GCN) under the irradiation of simulated visible light, respectively. The enhanced photocatalytic activity can be ascribed to the synergistic effect of nZVI and rGO to improve the separation of charge carriers and boost the harvest of visible light. The degradation mechanisms were explored by scavenger tests and X-ray photoelectron spectroscopy (XPS), indicating that holes (h+) played a dominant role in the decomposition of OFL, NOR, and CIP. The piperazine ring and C–N between the piperazine ring and benzene were the primary attack sites of h+. In addition, the ring-opening oxidation of benzene (C=C bond) connected by the C–F bond may also be an essential step. This study shed light on the degradation mechanism of OFL, NOR, and CIP under visible light irradiation of the 10% IGCN and provided theoretical support for the practical application of photocatalysis in treating antibiotics-containing water.
Potential physiological responses contributing to the ergogenic effects of acute ischemic preconditioning during exercise: A narrative review

Ischemic preconditioning (IPC) has been reported to augment exercise performance, but there is considerable heterogeneity in the magnitude and frequency of performance improvements. Despite a burgeoning interest in IPC as an ergogenic aid, much is still unknown about the physiological mechanisms that mediate the observed performance enhancing effects. This narrative review collates those physiological responses to IPC reported in the IPC literature and discusses how these responses may contribute to the ergogenic effects of IPC. Specifically, this review discusses documented central and peripheral cardiovascular responses, as well as selected metabolic, neurological, and perceptual effects of IPC that have been reported in the literature.
In situ organic biosignature detection techniques for space applications

The search for life in Solar System bodies such as Mars and Ocean Worlds (e.g., Europa and Enceladus) is an ongoing and high-priority endeavor in space science, even ∼ five decades after the first life detection mission at Mars performed by the twin Viking landers. However, the in situ detection of biosignatures remains highly challenging, both scientifically and technically. New instruments are being developed for detecting extinct or extant life on Mars and Ocean Worlds due to new technology and fabrication techniques. These instruments are becoming increasingly capable of both detecting and identifying in situ organic biosignatures that are indicative of life and will play a pivotal role in the search for evidence of life through robotic lander missions. This review article gives an overview of techniques used for space missions (gas chromatography, mass spectrometry, and spectroscopy), the further ongoing developments of these techniques, and ion mobility spectrometry. In addition, current developments of techniques used in the next-generation instruments for organic biosignature detection are reviewed; these include capillary electrophoresis, liquid chromatography, biosensors (primarily immunoassays), and nanopore sensing; whereas microscopy, biological assays, and isotope analysis are beyond the scope of this paper and are not covered.
Editorial: New omics research challenges for Public and sustainable Health

COPYRIGHT © 2022 Traversi and Ripabelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Editorial: New omics research challenges for Public and sustainable Health