Miracle-dz/UniFAR-FTUs huggingface dataset:
Targeting NPC1 in Renal Cell Carcinoma

Simple Summary The development of multi-targeted tyrosine kinase inhibitors (TKIs) and immunotherapeutic agents notably changed the treatment paradigm of advanced kidney cancer. However, despite the therapeutic progress, complete and durable responses have been noted in only a few cases. Our studies demonstrate that all major lipoproteins have a comparable ability to supply cholesterol to tumor cells and compromise the antitumor activity of TKIs. Endolysosomal cholesterol transport regulated by NPC1 protein is an attractive therapeutic target based on the fact that this is a point where LDL-, HDL-, and VLDL-derived cholesterol trafficking routes converge and therefore may be simultaneously targeted. Our studies elucidated the role of NPC1 as a potential therapeutic target in clear cell renal cell carcinoma (ccRCC). Abstract Rapidly proliferating cancer cells have a greater requirement for cholesterol than normal cells. Tumor cells are largely dependent on exogenous lipids given that their growth requirements are not fully met by endogenous pathways. Our current study shows that ccRCC cells have redundant mechanisms of cholesterol acquisition. We demonstrate that all major lipoproteins (i.e., LDL, HDL, and VLDL) have a comparable ability to support the growth of ccRCC cells and are equally effective in counteracting the antitumor activities of TKIs. The intracellular trafficking of exogenous lipoprotein-derived cholesterol appears to be distinct from the movement of endogenously synthesized cholesterol. De novo synthetized cholesterol is transported from the endoplasmic reticulum directly to the plasma membrane and to the acyl-CoA: cholesterol acyltransferase, whereas lipoprotein-derived cholesterol is distributed through the NPC1-dependent endosomal trafficking system. Expression of NPC1 is increased in ccRCC at mRNA and protein levels, and high expression of NPC1 is associated with poor prognosis. Our current findings show that ccRCC cells are particularly sensitive to the inhibition of endolysosomal cholesterol export and underline the therapeutic potential of targeting NPC1 in ccRCC.
From Handcraft to Digital: Transforming Spatial Crochet into Cutting-Edge Technology

In the past decade, additive manufacturing processes have exhibited immense potential by pushing the boundaries of material exploration. Involving the construction of volumetric objects through adding flat knitting machines has gained recognition as an additive manufacturing technology since they possess the unique capability to directly fabricate three-dimensional articles from yarn. Nevertheless, while flat knitting machines have made significant strides in the production of three-dimensional textiles, they still face hurdles in handling intricate structures, achieving large-scale production, and accommodating a wide range of yarns. This article proposes crochet as a feasible substitute for knitting in multi-scale applications, owing to its inherent ability to create seamless three-dimensional shells. Despite recent progress in computing crochet instructions, the absence of a digital fabrication technique for volumetric crochet within the framework of additive manufacturing remains a notable gap. This paper provides a thorough examination of the recent advances in computational crochet design tools. It then proceeds to analyze crocheting hand gestures and toolpaths to identify the pertinent design characteristics for a novel technology. Lastly, the paper introduces a pioneering digital fabrication device specifically designed for robotic crochet frameworks. This device underscores the notable advantages of crochet in comparison to other related fabrication methods.
Classification of autism spectrum disorder using electroencephalography in Chinese children: a cross-sectional retrospective study

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by diverse clinical features. EEG biomarkers such as spectral power and functional connectivity have emerged as potential tools for enhancing early diagnosis and understanding of the neural processes underlying ASD. However, existing studies yield conflicting results, necessitating a comprehensive, data-driven analysis. We conducted a retrospective cross-sectional study involving 246 children with ASD and 42 control children. EEG was collected, and diverse EEG features, including spectral power and spectral coherence were extracted. Statistical inference methods, coupled with machine learning models, were employed to identify differences in EEG features between ASD and control groups and develop classification models for diagnostic purposes. Our analysis revealed statistically significant differences in spectral coherence, particularly in gamma and beta frequency bands, indicating elevated long range functional connectivity between frontal and parietal regions in the ASD group. Machine learning models achieved modest classification performance of ROC-AUC at 0.65. While machine learning approaches offer some discriminative power classifying individuals with ASD from controls, they also indicate the need for further refinement.
Wideband Vertically Polarized Endfire Metasurface Antenna Fed by Tightly Coupled Monopole Probe Array

In this article, we proposed a wideband and vertically polarized endfire 2-D tapered metasurface (MS) antenna, which is fed by a tightly coupled monopole probe array (TCMPA). The TCMPA is a configuration consisting of a top-loaded short vertical monopole within a printed circuit board (PCB) stack between two ground planes. When appropriately designed, it exhibits a wideband characteristic by exploiting the operation principle of the tightly coupled dipole array (TCDA), which is well-suited for thin aperture-sized fifth-generation (5G) millimeter-wave (mmWave) antennas. In addition, the MS-based radiating structure was investigated through a dispersion diagram, which is imperative for impedance matching to the thin TCMPA in a PCB stack. A wideband operation of the TCMPA with the MS was demonstrated. A significant advantage of this is that the wideband operation of a very thin endfire antenna can be achieved using only a PCB: it was shown that the thickness of the proposed array is $0.06\lambda $ low at the lowest operating frequency. The impedance matching bandwidth of $1\times4$ array is 22.5–42.1 GHz (60.7%) for uniform excitation and 24.3–42.1 GHz (53.6%) for a scan angle of 45°. Experimental results, including total active reflection coefficient (TARC), realized array gain, and radiation pattern, were compared with simulated results, which were in good agreement. Finally, the comparative advantages of the proposed array were demonstrated compared to state-of-the-art 5G endfire linear array antennas.
CONFLICT MANAGEMENT IMPLEMENTATION (Case Study at SMPIT Bintang – South Tangerang City)

This article aims to give an overview of humans in an organization always faced with conflicts both individually and in groups. Conflict is inevitable. Every group in an organization in which there is interaction between one another is very prone to conflict. Conflicts can also occur within an Educational Institution. The implementation of conflict management turns out to be able to create a good working relationship in order to build mutual respect between fellow members of the organization and respect for diversity, to prevent disharmony between members of the organization, so as to focus on the vision and mission of the organization, to increase the creativity of the members of the organization by taking advantage of the conflicts that occur. The method of writing this article is descriptive qualitative based on a library research approach (literature study). The purpose of this study is to get an overview of the application of conflict management in SMPIT Bintang. In addition, the purpose of this study is to find out how the principal in optimizing the application of conflict management. The result of this research is that conflict management practices have been implemented by the Principal at SMPIT Bintang. The conflict management practice implemented by the Head of SMPIT Bintang refers to the theory of conflict management styles, namely by practicing the style of competition, collaboration, compromise, avoidance, accommodating.
Positional multi-length and mutual-attention network for epileptic seizure classification

The automatic classification of epilepsy electroencephalogram (EEG) signals plays a crucial role in diagnosing neurological diseases. Although promising results have been achieved by deep learning methods in this task, capturing the minute abnormal characteristics, contextual information, and long dependencies of EEG signals remains a challenge. To address this challenge, a positional multi-length and mutual-attention (PMM) network is proposed for the automatic classification of epilepsy EEG signals. The PMM network incorporates a positional feature encoding process that extracts minute abnormal characteristics from the EEG signal and utilizes a multi-length feature learning process with a hierarchy residual dilated LSTM (RDLSTM) to capture long contextual dependencies. Furthermore, a mutual-attention feature reinforcement process is employed to learn the global and relative feature dependencies and enhance the discriminative abilities of the network. To validate the effectiveness PMM network, we conduct extensive experiments on the public dataset and the experimental results demonstrate the superior performance of the PMM network compared to state-of-the-art methods.
Nuclear VANGL2 Inhibits Lactogenic Differentiation

Planar cell polarity (PCP) proteins coordinate tissue morphogenesis by governing cell patterning and polarity. Asymmetrically localized on the plasma membrane of cells, transmembrane PCP proteins are trafficked by endocytosis, suggesting they may have intracellular functions that are dependent or independent of their extracellular role, but whether these functions extend to transcriptional control remains unknown. Here, we show the nuclear localization of transmembrane, PCP protein, VANGL2, in the HCC1569 breast cancer cell line, and in undifferentiated, but not differentiated, HC11 cells that serve as a model for mammary lactogenic differentiation. The loss of Vangl2 function results in upregulation of pathways related to STAT5 signaling. We identify DNA binding sites and a nuclear localization signal in VANGL2, and use CUT&RUN to demonstrate recruitment of VANGL2 to specific DNA binding motifs, including one in the Stat5a promoter. Knockdown (KD) of Vangl2 in HC11 cells and primary mammary organoids results in upregulation of Stat5a, Ccnd1 and Csn2, larger acini and organoids, and precocious differentiation; phenotypes are rescued by overexpression of Vangl2, but not Vangl2ΔNLS. Together, these results advance a paradigm whereby PCP proteins coordinate tissue morphogenesis by keeping transcriptional programs governing differentiation in check.
A close look at a severe mesoscale convective syste m surrounding the Air France 447 crash

To investigate the degree of severity of cloud-top features of the severe Mesoscale Convective System (MCS) might be associated with the Air France (AF) Flight 447 crashed in the tropical Atlantic Ocean on 01 June 2009 from 02:00 to 02:15 UTC, we have used the multispectral data of Spinning Enhanced Visi ble and Infra-Red Imager (SEVIRI) onboard Meteosat-9 based on detection of overshooting tops. Two SEVIRI water vapor (WV) band images (6.2 �m and 7.3 �m) and the SEVRI thermal infrared (IR) band images (10.8 �m) provided information about structure and microphysi cs of MCS cloudiness. Overall, the results obtained suggest that existence of a severe Cloud cluster (MSC) on i ts route. A striking feature is that this Cloud clu ster resulted from the merging of four smaller clusters and its e xtension from west to east over around 400 km - one which could be potential responsible for the extremeness of the event by itself. Such clusters produced the coldest cloud tops (~minus 81 oC or colder) above MCS and organized into tight curved areas around the center (i.e., a doughnut or a cold-ring shaped form). As deep convection continued to developed, the disturbance gradu ally moved westward and began to break away from the MCS. Another conclusion is that the coldest pixels of convection (195K) exhibited brightness temperature difference (WV6.2-WV7.3) values as high +6. Thus, strong overshooting tops are often related to severe weath er or strong updraft.
Quantifying soil microbial effects on plant species coexistence: a conceptual synthesis

Soil microorganisms play a critical role in shaping biodiversity dynamics in plant communities. These microbial effects can arise through direct mediation of plant fitness by pathogens and mutualists, and over the past two decades, numerous studies have shined a spotlight on the role of dynamic feedbacks between plants and soil microorganisms as key determinants of plant species coexistence. Such feedbacks arise when plants species modify the composition of the soil community, which in turn affects plant performance. Stimulated by a theoretical model developed in the 1990s, a bulk of the empirical evidence for microbial controls over plant coexistence comes from experiments that quantify plant growth in soil communities that were previously conditioned by conspecific or het-erospecific plants. These studies have revealed that soil microbes can generate plant community dynamics ranging from strong negative frequency-dependence to strong positive frequency-dependence. Even as soil microbes have become recognized as a key player in determining plant coexistence outcomes, the past five years have seen a renewed interest in expanding the conceptual foundations of this field. New results include extensions of plant-soil feed-back theory to multi-species communities, re-interpretations of key metrics from classic two-species models, and frameworks to integrate plant-soil feedbacks with processes like intra-and inter-specific competition. Here, I review the implications of theoretical developments for interpreting existing empirical results, and highlight proposed designs for future experiments that can reinforce key model assumptions and enable a more complete understanding of microbial regulation of plant community dynamics.
The European Network for Translational Research in Atrial (EUTRAF): Objectives and Initial Results

: Atrial fibrillation (AF) is the most common sustained arrhythmia in the general population. As an age-related arrhythmia AF is becoming a huge socio-economic burden for European healthcare systems. Despite significant progress in our understanding of the pathophysiology of AF therapeutic strategies for AF have not changed substantially and the major challenges in the management of AF are still unmet. This lack of progress may be related to the multifactorial pathogenesis of atrial remodeling and AF that hampers the identification of causative pathophysiological alterations in individual patients. Also, again new mechanisms have been identified and the relative contribution of these mechanisms still has to be established. In November 2010, the European Union launched the large collaborative project EUTRAF (=European Network of Translational Research in Atrial Fibrillation) to address these challenges.
Digital System Design - Use of Microcontroller

Embedded systems are today, widely deployed in just about every piece of machinery from toasters to spacecraft. Embedded system designers face many challenges. They are asked to produce increasingly complex systems using the latest technologies, but these technologies are changing faster than ever. They are asked to produce better quality designs with a shorter time-to-market. They are asked to implement increasingly complex functionality but more importantly to satisfy numerous other constraints. To achieve the current goals of design, the designer must be aware with such design constraints and more importantly, the factors that have a direct effect on them. One of the challenges facing embedded system designers is the selection of the optimum processor for the application in hand; single-purpose, general-purpose or application specific. Microcontrollers are one member of the family of the application specific processors. The book concentrates on the use of microcontroller as the embedded system's processor, and how to use it in many embedded system applications. The book covers both the hardware and software aspects needed to design using microcontroller. The book is ideal for undergraduate students and also the engineers that are working in the field of digital system design.
URBAN AND SPATIAL DEVELOPMENT ACROSS GEOGRAPHIES

Making the analysis of urban and spatial development more acceptable by all levels of analysis and governance requires a multi-level – or in other words vertical – approach to indicators measuring development paths. The 2030 Agenda of the United Nations with its 17 Sustainable De - velopment Goals offers a promising chance to establish, maintain, and further develop a monitoring system that is supported by all levels involved, as well as all stakeholder groups and individuals across all levels. First experiences gathered in a nation (Germany) and bilateral and supranational
Effect of early intravenous calcium replacement after total thyroidectomy

Aim: This study was to evaluate the need for postoperative calcium replacement in patients undergoing total thyroidectomy, to evaluate the effect of intravenous calcium therapy given in the early postoperative period regardless of serum calcium level and physical examination on the length of stay of the patients, parathormone levels, and the need for oral calcium after discharge. Materials and Methods: The files of 73 patients who underwent bilateral total thyroidectomy between January 2018 and July 2023 were retrospectively reviewed, and the demographic characteristics, anti-TG, anti-TPO, postoperative calcium, parathormone, thyroid gland weight of the patients were recorded. The effect of intravenous calcium replacement on calcium requirement, parathormone levels, and length of hospitalization was evaluated independently of calcium levels. Results: It has been observed that replacement is associated with long-term calcium requirement in patients who underwent bilateral total thyroidectomy and received intravenous calcium in the early period. The length of stay was found to be longer in patients who underwent calcium replacement. Conclusion: Various treatment methods are used to prevent hypocalcemia. One of these treatment methods is early intravenous calcium replacement. In clinical practice, treatments given to prevent the development of complications and to discharge patients early may sometimes lead to the occurrence of complications that are tried to be prevented. The purpose of calcium replacement is to prevent the development of hypocalcemia, to reduce the complication rate and to provide early discharge. It was evaluated that intravenous calcium replacement therapy, which was applied in the early period to prevent postoperative hypocalcemia, caused patients to need longer-term calcium replacement and prolonged hospitalization. It is important to avoid calcium replacement in the early period and to evaluate the physical examination findings along with the serum calcium level of the patients for replacement.
ACOUSTIC PROPERTIES OF THE FOUR-WAY LARYNGEAL CONTRAST IN BENGALI INFANT DIRECTED SPEECH

This paper analyzes the acoustic properties of the four-way laryngeal contrast in Infant Directed Speech (IDS), comparing it with Adult Directed Speech (ADS). Since IDS has been demonstrated to be hyper-articulated, its acoustic manifestation is expected to contain enhanced cues to the four-way contrast. I investigated VOT, PVI and H1*– H2* of prevocalic stops and affricates in the speech of 10 Bangladeshi Bengali speakers. The results revealed an asymmetry in IDS – the VOT of voiced-unaspirated and voiced-aspirated segments was longer in IDS, but there was no difference in the VOT of voiceless-aspirated and voiceless-unaspirated segments between the two registers; PVI of voiced-aspirated segments, but not that of voiceless-aspirated segments, was longer in IDS. Finally, H1*–H2* did not differ by register, although the expected effect of category was found. Viewed against studies on similar languages, these results suggest that language-specific phonetic grammars modulate the acoustic manifestation of IDS.
Time‐Lapse Electrical Imaging of Water Infiltration in the Context of Soil Aquifer Treatment

We investigated geoelectrical monitoring of water infiltration within soil aquifer treatment. Time‐lapse ERT is able to track water infiltration under very high recurrent fluxes. Individual apparent resistivity captures temporal dynamics of water infiltration. Spatiotemporal ERT monitoring results used for hydraulic subsurface zonation.
Impacts of light pollution on bat spatiotemporal dynamics in France : implications for outdoor lighting planning

Light pollution induced by the widespread use of nighttime artificial lighting is a global change affecting substantial part of terrestrial and marine ecosystems. As a result, major concerns have been raised about its hidden impacts on biodiversity and ecosystem functioning. Light pollution has major impacts on the circadian and seasonal cycles of organisms, and on their movements and spatial distributions. As a whole, light pollution likely disrupts the spatiotemporal dynamics of biological communities and ecosystems. In this context, the aim of this PhD was to characterize the impacts of nighttime artificial lighting on bat activity (order: chiroptera) at multiple spatial scales in order to propose reduction measures that can effectively limit the adverse impacts of light pollution on biodiversity. We used bats as model species as they are nocturnal and directly exposed to light pollution and they are considered to be good indicators of the response of biodiversity to anthropogenic pressure.We first intended to characterize the extent of effect of light pollution at a landscape scale relative to major land-use pressures that are threatening biodiversity worldwide. Using a French national-scale citizen science database, we found that landscape-scale level of light pollution negatively affected common bat species, and that this effect was significantly stronger than the effect of impervious surfaces but weaker than the effect of intensive agriculture. This highlighted the crucial need to account for outdoor lighting in land-use planning in order to restore darkness in human-inhabited landscapes.Thus, through an in situ experiment, we investigated whether i) restoring darkness in a landscape for a part of the night through part-night lighting schemes, or ii) restraining the spatial extent of lighting at the vicinity of natural elements were effective options to enhance dark ecological corridors in human-inhabited landscapes. We found that part-night lighting schemes were unlikely to effectively mitigate the impacts of artificial lighting on light-sensitive species. However, we revealed that streetlights should be separated from ecological corridors by at least 50 m, and that the light trespass should be lower than 0.1 lux to allow their effective use by light-sensitive species.Overall, this PhD thesis revealed the major importance of addressing light pollution issues at multiple spatial scales to characterize its impacts on biodiversity. It also exposed the crucial importance of integrating outdoor lighting in land-use planning strategies and proposed to implement ecological criteria in future European standards for outdoor lighting.