Miracle-dz/UniFAR-FTUs huggingface dataset:
Applications of diffusion tensor imaging integrated with neuronavigation to prevent visual damage during tumor resection in the optic radiation area

Background Intracranial tumors involving the temporo-occipital lobe often compress or destroy the optic radiation (OpR), resulting in decreased visual function. The aim of this study is to explore the value of diffusion tensor imaging (DTI) tractography integrated with neuronavigation to prevent visual damage when resecting tumors involving the OpR and find potential factors affecting patients’ visual function and quality of life (QOL). Methods Our study is a cross-sectional study that included 28 patients with intracranial tumors in close morphological relationship with the OpR recruited between January 2020 and February 2022. The surgical incision and approach were preoperatively designed and adjusted according to the DTI tractography results and visual function scores. All patients underwent examinations of visual acuity (VA) and visual field index (VFI) and completed visual function and QOL scales at admission and 2 months after discharge. Logistic regression and linear regression analysis were conducted to evaluate clinical factors potentially affecting pre/postoperative OpR morphology, VA, VFI, visual function, and QOL. Results Lesion size was the main factor found to affect visual function (β = -0.74, 95%CI: -1.12~-0.36, P = 0.05), VA (left: β = -0.11, 95%CI: -0.14~-0.08, P < 0.001; right: β = -0.15, 95%CI: -0.17~-0.13, P < 0.001), and VFI (left: β = -0.11, 95%CI: -0.14~-0.08, P < 0.001; right: β = -0.14, 95%CI: -0.16~-0.12, P < 0.001). Lesion size, edema, and involvement of the lateral ventricle temporal horn were factors affecting OpR morphology and QOL. The 28 patients showed significantly improved VA, VFI, visual function, and QOL results (P < 0.05) 2 months after discharge. Conclusions Combining DTI of OpR mapping and microscopic-based neuronavigation aided precise mapping and thus preservation of visual function in patients undergoing tumor resection. Potential clinical factors affecting patients’ visual function and QOL scores were identified which are useful for assessing a patient’s condition and predicting prognosis.
Petrogenesis of Havre Volcano in the Kermadec Arc: 2012 Eruption of a Chemically Homogeneous Rhyolite

The 2012 Havre submarine eruption produced a 1.5 km3 bulk rock volume or 0.52 km3 dense rock equivalent volume of rhyolite emplaced as minor lava flows, a field of sunken seafloor pumice, and a volumetrically dominant pumice raft. This moderately large volume of medium-K (1.4–1.6 wt% K2O) rhyolite pumice is relatively chemically homogeneous (71.5–73.0 wt% SiO2), and no trace element variation or cryptic zoning has been detected despite the textural diversity of pumice material. Radiogenic isotope ratios (87Sr/86Sr 0.703693–0.703744; 206Pb/204Pb 18.7648–18.7781; 208Pb/204Pb 38.587–38.605; 143Nd/144Nd 0.513001–0.513020) demonstrate the Havre rhyolite is sourced from mantle similar to regional eruptive products of the Kermadec arc volcanic front. Providing some further insight into the Havre magmatic system is an abundance of diverse volcanic rock fragments primarily embedded in the banded raft pumice. Embedded rock fragments represent a variety of fresh to hydrothermally altered lavas ranging in composition from basaltic to rhyolitic (50.6–72.3 wt% SiO2) and are likely sourced from varying depths within the volcanic conduit during explosive fragmentation. The diverse embedded volcanic rock fragments, therefore, represent earlier erupted lavas that constructed Havre volcano and are snapshots of the petrogenetic history of Havre. Magnesian augite in basaltic to basaltic andesite embedded rock fragments has a similar compositional range (En55Fs12Wo33 to En39Fs26Wo35) to the previously documented antecrystic clinopyroxene observed in the 2012 rhyolite pumice raft. Herein, we explain how this large volume of chemically homogeneous crystal-poor rhyolite can be generated in an oceanic arc setting based on major and trace element petrogenetic models. Rhyolite-MELTS crystal fractionation models indicate the antecrystic mineral compositions within the Havre pumice of plagioclase (An55–78), and magnesian augites (En53Fs10Wo37 to En40Fs26Wo34) are the primary phases that would crystallize in basaltic to andesitic melt compositions. Modeling indicates that the forerunner basaltic magma must be a relatively dry (∼1 wt% H20) low-K tholeiitic basalt in composition and would require ∼78% crystallization at different pressures to ultimately generate the Havre 2012 rhyolite.
Spatio-temporal variations of ecosystem service values in response to land use/cover change in Luoyang city

Land-use/cover change (LUCC) caused by human activities is an important factor in changes in global ecosystems; however, the impact of LUCC on ecosystem service value (ESV) has never been previously assessed in Luoyang City, China. This paper explores spatial and temporal evolution patterns of land use pattern change, ESV change and response to LUCC in Luoyang City from 2010 to 2019 through GIS and remote sensing techniques, and employing transfer matrix, ESV spatial autocorrelation analysis and elastic coefficient change. During the study period: 1) Between 2010 and 2019, land-use changes in Luoyang City showed a trend of “two increases and four decreases,” specifically, built-up land and forest areas increased, while the extent of cropland, grassland, water, and unused land areas decreased. 2) The recorded land-use conversion in Luoyang City is complex, with forest and cropland land uses increasing and decreasing fastest, respectively, with the proportion of forest increasing by 10.19% and cropland decreasing by 3.37% — the reason for this change is the transition from cropland and grassland to forest and occupation of cropland by built-up land. 3) The ESV change in Luoyang City was U-shaped, with the total ESV increasing 1.96% from ¥44.78 billion to ¥45.66 billion; additionally, the ESV provided by forest areas increased by 23.83%. 4) From 2010 to 2019, Luoyang’s hydrological regulation, climate regulation and soil and water conservation contributed the most to Luoyang’s ESV, accounting for 63.89% of the total ESV in 2019. 5) The overall ESVs of the townships in Luoyang City have obvious clustering and are not completely randomly spatially distributed. With the optimization of land-use allocation, the ESV high–high clusters have expanded. 6) The overall elasticity coefficient is high in Luoyang City and ESV responds intensely to LUCC, with the most significant response shown by the forests of Luolong District. The findings of this survey are practical for helping Luoyang City to optimize land resource usage scientifically.
Policies of voluntary services involved in public health emergencies in China: Evolution, evaluation, and expectation

Background Public health emergencies have an immense effect on social stability, economic development, and human life. Volunteers played an indispensable role in health monitoring, assistance with daily life, and social network repairing. However, few policies analysis concentrated on the voluntary services involved in public health emergencies. Objectives This study aims to analyze and summarize the advantages and flaws of the policy documents relating to voluntary services involved in public health emergencies, and put forward the enlightenment on policymaking and optimization. Methods A three-dimensional analysis framework of “Policy instruments—Participants of voluntary services—Stages of voluntary services” was designed. Policy documents at the national level were retrieved from the official websites of the State Council of the People's Republic of China and its departments as well as the PKULAW Database. NVivo software was applied to analyze the contents of the included policy documents. Gephi software was adopted to conduct a visualized atlas analysis of the cooperative network among policymaking departments. Results A total of 77 policy documents were included, and most were published in 2020 (n = 40). The Ministry of Civil Affairs (n = 19) and the National Health Commission (n = 18) issued more documents than the other departments. They cooperated more extensively with other departments. In policy documents, supply-side policy instruments were utilized the most (65.4%), followed by demand-side (23.9%). Voluntary organizations in the form of ambiguous sense were most mentioned as service participants (n = 73). In the stages of service delivery, service content mainly involved the prevention and control of public health emergencies (27, 18.9%) and psychological counseling (26, 18.2%). Conclusion Time distribution of policy documents featured “incubation period—outbreak and continuous evolution period—elimination recovery period. ” Joint issuing became the dominant form. The internal structure of policy instruments was unbalanced with different priorities, and the overall structure is expected to be optimized to promote voluntary organization management, reinforce external resources, and close the gap between policymaking and policy implementation. Volunteers' competence and voluntary organizations' system needs to be improved, and the contents of voluntary services should be enriched for the preparedness for future public health emergency.
On doing multi-act arithmetic: A multitrait-multimethod approach of performance dimensions in integrated multitasking

Here we present a systematic plan to the experimental study of test–retest reliability in the multitasking domain, adopting the multitrait-multimethod (MTMM) approach to evaluate the psychometric properties of performance in Düker-type speeded multiple-act mental arithmetic. These form of tasks capacitate the experimental analysis of integrated multi-step processing by combining multiple mental operations in flexible ways in the service of the overarching goal of completing the task. A particular focus was on scoring methodology, particularly measures of response speed variability. To this end, we present data of two experiments with regard to (a) test–retest reliability, (b) between-measures correlational structure, (c) and stability (test–retest practice effects). Finally, we compared participants with high versus low performance variability to assess ability-related differences in measurement precision (typically used as proxy to “simulate” patient populations), which is especially relevant in the applied fields of clinical neuropsychology. The participants performed two classic integrated multi-act arithmetic tasks, combining addition and verification (Exp. 1) and addition and comparison (Exp. 2). The results revealed excellent test–retest reliability for the standard and the variability measures. The analysis of between-measures correlational structure revealed the typical pattern of convergent and discriminant relationships, and also, that absolute response speed variability was highly correlated with average speed (r > 0.85), indicating that these measures mainly deliver redundant information. In contrast, speed-adjusted (relativized) variability revealed discriminant validity being correlated to a much lesser degree with average speed, indicating that this measure delivers additional information not already provided by the speed measure. Furthermore, speed-adjusted variability was virtually unaffected by test–retest practice, which makes this measure interesting in situations with repeated testing.
Dissociable effects of music and white noise on conflict-induced behavioral adjustments

Auditory stimuli, encompassing a continually expanding collection of musical genres and sonic hues, present a safe and easily administrable therapeutic option for alleviating cognitive deficits associated with neuropsychological disorders, but their effects on executive control are yet to be completely understood. To better understand how the processing of certain acoustic properties can influence conflict processing, we had a large of cohort of undergraduate students complete the Stroop colour and word test in three different background conditions: classical music, white noise, and silence. Because of pandemic guidelines and the necessity to run the experiment remotely, participants also completed the Wisconsin card sorting test (WCST), so that the reliability and consistency of acquired data could be assessed. We found that white noise, but not classical music increased the response time difference between congruent (low conflict) and incongruent (high conflict) trials (conflict cost), hence impairing performance. Results from the WCST indicated that home-based data collection was reliable, replicating a performance bias reported in our previous laboratory-based experiments. Both the auditory stimuli were played at a similar intensity, thus their dissociable effects may have resulted from differing emotional responses within participants, where white noise, but not music elicited a negative response. Integrated with previous literature, our findings indicate that outside of changes in tempo and valence, classical music does not affect cognitive functions associated with conflict processing, whilst white noise impairs these functions in a manner similar to other stressors, and hence requires further research before its implementation into neuropsychiatric care.
Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling

Strategies based on intracellular expression of artificial binding domains present several advantages over manipulating nucleic acid expression or the use of small molecule inhibitors. Intracellularly-functional nanobodies can be considered as promising macrodrugs to study key signaling pathways by interfering with protein-protein interactions. With the aim of studying the RAS-related small GTPase RHOA family, we previously isolated, from a synthetic phage display library, nanobodies selective towards the GTP-bound conformation of RHOA subfamily proteins that lack selectivity between the highly conserved RHOA-like and RAC subfamilies of GTPases. To identify RHOA/ROCK pathway inhibitory intracellular nanobodies, we implemented a stringent, subtractive phage display selection towards RHOA-GTP followed by a phenotypic screen based on F-actin fiber loss. Intracellular interaction and intracellular selectivity between RHOA and RAC1 proteins was demonstrated by adapting the sensitive intracellular protein-protein interaction reporter based on the tripartite split-GFP method. This strategy led us to identify a functional intracellular nanobody, hereafter named RH28, that does not cross-react with the close RAC subfamily and blocks/disrupts the RHOA/ROCK signaling pathway in several cell lines without further engineering or functionalization. We confirmed these results by showing, using SPR assays, the high specificity of the RH28 nanobody towards the GTP-bound conformation of RHOA subfamily GTPases. In the metastatic melanoma cell line WM266-4, RH28 expression triggered an elongated cellular phenotype associated with a loss of cellular contraction properties, demonstrating the efficient intracellular blocking of RHOA/B/C proteins downstream interactions without the need of manipulating endogenous gene expression. This work paves the way for future therapeutic strategies based on protein-protein interaction disruption with intracellular antibodies.
Retinal microglia protect against vascular damage in a mouse model of retinopathy of prematurity

Retinopathy of prematurity (ROP) is a common cause of blindness in preterm babies. As a hypoxia-induced eye disease characterized by neovascularization, its association with retinal microglia has been noted but not well documented. We performed a comprehensive analysis of retinal microglia and retinal vessels in mouse oxygen-induced retinopathy (OIR), an animal model of ROP. In combination with a pharmacological inhibitory strategy, the role of retinal microglia in vascular network maintenance was investigated. Postnatal day (P) 7 C57BL/6J mouse pups with their nursing mother were exposed to 75% oxygen for 5 days to induce OIR. Age-matched room air-treated pups served as controls. On P12, P17, P21, P25, and P30, retinal microglia and vessels were visualized and quantified based on their location and activation status. Their relationship with retinal vessels was also analyzed. On P5 or P12, retinal microglia inhibition was achieved by intravitreal injection of liposomes containing clodronate (CLD); retinal vasculature and microglia were examined in P12 and P17 OIR retinae. The number of retinal microglia was increased in the superficial areas of OIR retinae on P12, P17, P21, P25, and P30, and most of them displayed an amoeboid (activated) morphology. The increased retinal microglia were associated with increased superficial retinal vessels in OIR retinae. The number of retinal microglia in deep retinal areas of OIR retinae also increased from P17 to P30 with a ramified morphology, which was not associated with reduced retinal vessels. Intravitreal injection of liposomes-CLD caused a significant reduction in retinal microglia. Loss of retinal microglia before hyperoxia treatment resulted in increased vessel obliteration on P12 and subsequent neovascularization on P17 in OIR retinae. Meanwhile, loss of retinal microglia immediately after hyperoxia treatment on P12 also led to more neovascularization in P17 OIR retinae. Our data showed that activated microglia were strongly associated with vascular abnormalities upon OIR. Retinal microglial activation continued throughout OIR and lasted until after retinal vessel recovery. Pharmacological inhibition of retinal microglia in either hyperoxic or hypoxic stage of OIR exacerbated retinal vascular consequences. These results suggested that retinal microglia may play a protective role in retinal vasculature maintenance in the OIR process.
Disease similarity network analysis of Autism Spectrum Disorder and comorbid brain disorders

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder with heterogeneous clinical presentation, variable severity, and multiple comorbidities. A complex underlying genetic architecture matches the clinical heterogeneity, and evidence indicates that several co-occurring brain disorders share a genetic component with ASD. In this study, we established a genetic similarity disease network approach to explore the shared genetics between ASD and frequent comorbid brain diseases (and subtypes), namely Intellectual Disability, Attention-Deficit/Hyperactivity Disorder, and Epilepsy, as well as other rarely co-occurring neuropsychiatric conditions in the Schizophrenia and Bipolar Disease spectrum. Using sets of disease-associated genes curated by the DisGeNET database, disease genetic similarity was estimated from the Jaccard coefficient between disease pairs, and the Leiden detection algorithm was used to identify network disease communities and define shared biological pathways. We identified a heterogeneous brain disease community that is genetically more similar to ASD, and that includes Epilepsy, Bipolar Disorder, Attention-Deficit/Hyperactivity Disorder combined type, and some disorders in the Schizophrenia Spectrum. To identify loss-of-function rare de novo variants within shared genes underlying the disease communities, we analyzed a large ASD whole-genome sequencing dataset, showing that ASD shares genes with multiple brain disorders from other, less genetically similar, communities. Some genes (e.g., SHANK3, ASH1L, SCN2A, CHD2, and MECP2) were previously implicated in ASD and these disorders. This approach enabled further clarification of genetic sharing between ASD and brain disorders, with a finer granularity in disease classification and multi-level evidence from DisGeNET. Understanding genetic sharing across disorders has important implications for disease nosology, pathophysiology, and personalized treatment.
Genome wide association study to detect genetic regions related to isoflavone content in a mutant soybean population derived from radiation breeding

Isoflavones are major secondary metabolites that are exclusively produced by legumes, including soybean. Soy isoflavones play important roles in human health as well as in the plant defense system. The isoflavone content is influenced by minor-effect quantitative trait loci, which interact with polygenetic and environmental factors. It has been difficult to clarify the regulation of isoflavone biosynthesis because of its complex heritability and the influence of external factors. Here, using a genotype-by-sequencing-based genome-wide association mapping study, 189 mutant soybean genotypes (the mutant diversity pool, MDP) were genotyped on the basis of 25,646 high-quality single nucleotide polymorphisms (SNPs) with minor allele frequency of >0.01 except for missing data. All the accessions were phenotyped by determining the contents of 12 isoflavones in the soybean seeds in two consecutive years (2020 and 2021). Then, quantitative trait nucleotides (QTNs) related to isoflavone contents were identified and validated using multi-locus GWAS models. A total of 112 and 46 QTNs related to isoflavone contents were detected by multiple MLM-based models in 2020 and 2021, respectively. Of these, 12 and 5 QTNs were related to more than two types of isoflavones in 2020 and 2021, respectively. Forty-four QTNs were detected within the 441-Kb physical interval surrounding Gm05:38940662. Of them, four QTNs (Gm05:38936166, Gm05:38936167, Gm05:38940662, and Gm05:38940717) were located at Glyma.05g206900 and Glyma.05g207000, which encode glutathione S-transferase THETA 1 (GmGSTT1), as determined from previous quantitative trait loci annotations and the literature. We detected substantial differences in the transcript levels of GmGSTT1 and two other core genes (IFS1 and IFS2) in the isoflavone biosynthetic pathway between the original cultivar and its mutant. The results of this study provide new information about the factors affecting isoflavone contents in soybean seeds and will be useful for breeding soybean lines with high and stable concentrations of isoflavones.