Miracle-dz/UniFAR-FTUs huggingface dataset:
Reconstruction of molecular evolution of human influenza A H1N1/2009 virus in Iran and neighboring countries

In this study, the time and path of transmission of H1N1 serotype influenza A viruses in Iran and neighboring countries have been investigated by using Bayesian phylogeography analysis on the sequences extracted from the gene bank. We obtained all hemagglutinin (HA) and neuraminidase (NA) nucleotide sequences of influenza H1N1 available up to December 25, 2020, from Iran and its neighboring countries (i.e., Pakistan, Afghanistan, Turkmenistan, Armenia, Azerbaijan, Turkey, and Iraq). We also performed a Bayesian Markov chain Monte Carlo method to infer the evolutionary dynamic and the most recent common ancestor for the HA and NA sequences. Based on the extracted sequences, the age of emergence of H1N1 influenza virus serotype was older in Iran compared to neighboring countries, and with some degree of uncertainty, it seems Tehran had a key role and epicenter of transmission to other cities within Iran. The mean time of the most recent common ancestor of H1N1 viruses was 1989 (95% HPD: 1980–1994) for HA and NA as well. Along with ordinary measures like resource management, diagnostic approaches, and preparedness to fight against viruses that were in place, continuous monitoring, and screening of H1N1 serotype influenza virus in the country, especially by implementation of feasible, effective, and innovative measures at borderline should be initiated and identified gaps and shortage that should be a priority for virus control. It is also important for countries to have a regional monitoring program in addition to internal monitoring programs, as well as to start a virus molecular care program.
PI(4,5)P2-stimulated positive feedback drives the recruitment of Dishevelled to Frizzled in Wnt–β-catenin signaling

In the Wnt–β-catenin pathway, Wnt binding to Frizzled (Fzd) and LRP5 or LRP6 (LRP5/6) co-receptors inhibits the degradation of the transcriptional coactivator β-catenin by recruiting the cytosolic effector Dishevelled (Dvl). Polymerization of Dvl at the plasma membrane recruits the β-catenin destruction complex, enabling the phosphorylation of LRP5/6, a key step in inhibiting β-catenin degradation. Using purified Fzd proteins reconstituted in lipid nanodiscs, we investigated the factors that promote the recruitment of Dvl to the plasma membrane. We found that the affinity of Fzd for Dvl was not affected by Wnt ligands, in contrast to other members of the GPCR superfamily for which the binding of extracellular ligands affects the affinity for downstream transducers. Instead, Fzd-Dvl binding was enhanced by increased concentration of the lipid PI(4,5)P2, which is generated by Dvl-associated lipid kinases in response to Wnt and which is required for LRP5/6 phosphorylation. Moreover, binding to Fzd did not promote Dvl DEP domain dimerization, which has been proposed to be required for signaling downstream of Fzd. Our findings suggest a positive feedback loop in which Wnt-stimulated local PI(4,5)P2 production enhances Dvl recruitment and further PI(4,5)P2 production to support Dvl polymerization, LRP5/6 phosphorylation, and β-catenin stabilization. Description The phospholipid PI(4,5)P2 promotes Wnt signaling by enhancing the recruitment of Dishevelled to the membrane. Phospholipid-driven Wnt signaling Wnt stimulation of the receptor Fzd, a member of the G protein–coupled receptor (GPCR) superfamily, stabilizes the transcription factor β-catenin through a mechanism that depends on the recruitment of the cytosolic effector Dvl to Fzd and on PI(4,5)P2 generated by lipid kinases associated with Dvl. Using assays in lipid nanodiscs and in cells, Mahoney et al. showed that the recruitment of Dvl to Fzd required PI(4,5)P2 and occurred even in the absence of ligand. In contrast to other GPCRs for which ligand-induced conformational changes stimulate the recruitment of downstream G proteins, the presence of ligands did not increase the affinity of Fzd for Dvl. Thus, high Fzd-Dvl affinity appears to rely on local ligand-induced increases in the production of PI(4,5)P2 by Dvl-associated lipid kinases rather than a ligand-induced conformational change in Fzd (see the Focus by Angers).
Roles of socioeconomic status, ethnicity and teacher beliefs in academic grading

Abstract Background Educational outcomes in the United Kingdom vary as a function of students' family background, with those of lower socioeconomic status (SES) and certain ethnic minority groups among the worst affected. Aims This pre‐registered study investigates: (i) whether knowledge about students' socioeconomic and ethnic background influences teachers' judgements about the quality of their work and potential for the future, and (ii) the role of teachers' beliefs—most notably about meritocracy—in their practices. Sample Our findings are based on the responses of 416 in‐service (88%) and trainee (12%) teachers who successfully passed several stringent exclusion criteria. Methods As part of a 2 × 2 independent measures design, teachers were randomly assigned to assess an identical piece of work ostensibly written by a student who varied by SES (higher vs. lower) and ethnicity (White British vs. Black Caribbean). Following this, they responded to several measures assessing their beliefs about education. Results Teachers judged students of lower SES to be inferior to students of higher SES across a range of indicators. By contrast, we found no evidence of racial bias in teachers' judgements, though potential reasons for this are discussed. Teachers who believed that schooling is meritocratic were significantly less likely to support equity‐enhancing teaching practices and initiatives. Conclusions Unconscious teacher biases and beliefs may be contributing to the relative underperformance of students from poorer backgrounds. These findings provide a mandate for educational institutions to help teachers reflect upon, and develop the skills required to mitigate potentially harmful biases.
Freshwater Sediment Toxicity Evaluation from Meso‐Scale Spill Tests of Diluted Bitumen and Conventional Crude

Oil and gas development and transportation in many areas of the world, such as the oil sands region of Alberta, Canada, are heavily monitored to minimize the environmental impacts of development and the risk of oil spills. However, oil spills to aquatic environments still occur. Although the science of oil spills has received considerable attention of late, uncertainty still remains in evaluating the fate and transport of oil spills as well as the effects of those spills on aquatic biota. Experiments using meso‐scale spill tanks were used to examine the physical and chemical behavior of two types of oil, conventional crude (CC) and diluted bitumen (DB), under similar environmentally relevant scenarios (i.e., volume of spill, temperature, duration, wave action, and presence of river sediment). In addition, biological impact assessments via sediment toxicity testing collected from the oil spill tests were conducted. Sediments were evaluated for acute toxicity using three standard sediment test species: Hyalella azteca, Lumbriculus variegatus, and Chironomus dilutus. Sediments collected from the CC simulated spill showed a higher level of acute toxicity than sediments collected from spills with DB. Higher toxicity in the CC‐contaminated sediment was supported by higher concentrations of low molecular weight polycyclic aromatic hydrocarbons (PAHs) when compared with the DB‐contaminated sediment, while the remaining PAH profile was similar between the contaminated sediments. The use of a meso‐scale spill tank in combination with sediment bioassays allowed for the evaluation of oil spills under controlled and environmentally relevant conditions (e.g., nearshore high sediment loading river), and in turn provides assessors with additional information to identify the appropriate mitigation and remediation efforts needed in the event of future spills. Environ Toxicol Chem 2022;41:2797–2807. © 2022 SETAC
Strategies for immortalisation of amnion‐derived mesenchymal and epithelial cells

Mesenchymal (human amniotic mesenchymal stem cell [HAMSC]) and epithelial cells (human amnion epithelial cell [HAEC]) derived from human amniotic membranes possess characteristics of pluripotency. However, the pluripotency of HAMSC and HAEC are sustained only for a finite period. This in vitro cell growth can be extended by cell immortalisation. Many well‐defined immortalisation systems have been used for artificially overexpressing genes such as human telomerase reverse transcriptase in HAMSC and HAEC leading to controlled and prolonged cell proliferation. In recent years, much progress has been made in our understanding of the cellular machinery that regulates the cell cycle when immortalised. This review summarises the current understanding of molecular mechanisms that contribute to cell immortalisation, the strategies that have been employed to immortalise amnion‐derived cell types, and their likely applications in regenerative medicine.
Association of udder type traits with single nucleotide polymorphisms in Sahiwal (Bos indicus) and Karan Fries (Bos taurus × Bos indicus) cattle

Abstract Udder structure of milking cows is not merely vital to display the visual characteristics, but also important for high milk output and low mastitis risk incidence as well. The present study measured different udder type traits in Sahiwal (Bos indicus) and Karan Fries (Bos taurus × Bos indicus) and investigated their association with single nucleotide polymorphisms in Vitamin D Receptor and Protein Tyrosine Phosphatase, Receptor Type, R genes. GG genotype of SNP rs454303072 was found to have wider rear udder, larger udder circumference, longer distance between fore-rear teats and left-right teats in Karan Fries cattle. Whereas, in Sahiwal cattle, AA genotype of this SNP was found to be associated with the higher and wider rear udder, larger udder circumference and wider udder. AA genotype of SNP rs382671389 was found to be associated with longer fore teat in Karan Fries cattle. The TT and CC genotype of SNP rs435289107 was associated with udder type traits in Karan Fries and Sahiwal cattle respectively. These results suggest that BTA 5 harbors genomic regions associated with udder traits in Bos indicus and Bos indicus x Bos taurus cattle.
A latent class analysis to identify socio-economic and health risk profiles among mothers of young children predicting longitudinal risk of food insecurity

Background The purpose of the current study was to use a social determinants of health (SDOH) framework and latent class analysis (LCA) to identify risk classes among mothers with young children. The risk classes were then used to predict food insecurity severity and stability/change of food insecurity over time. Method The secondary data from the Fragile Families and Child Wellbeing Study (n = 2,368; oversampled for non-marital births) was used in this study. Household food insecurity was assessed using the 18-items USDA Food Security Survey. A seventeen-item inventory of educational, economic stability, incarceration (i.e. social context), neighborhood safety (i.e. neighborhood and built environment), health and health care, and substance use behaviors at baseline/Year-1 were included to identify SDOH risk indicators in the LCA. Covariate-adjusted multinomial logistic regression models were used to examine the relation between risk classes at Year-1 and the severity of food insecurity at Year-3 and stability/change of food insecurity between Year-3 and Year -5. Results LCA identified five risk classes: High utility and medical hardship (Class 1), high housing and employment hardship, high substance use, and incarceration (Class 2), high housing and medical hardship, poor health, and health care (Class 3), high employment hardship and low-income (Class 4) and low-risk (Class 5). The Class 1, Class 2 and Class 3 had greater odds of low food security and very low food security at Year-3 compared to Class 4. In addition, compared to Class 4, Class 1, Class 2 and Class 3 had greater odds unstable food insecurity and persistent food insecurity over time. Conclusions LCA could be used to identify distinctive family system risk profiles predictive of food insecurity. The generated risk profiles could be used by health care providers as an additional tool to identify families in need for resources to ensure household food security.
Progress on the 150-Inch Anglo-Australian Telescope

In April 1967 the British and Australian Governments agreed jointly to build and operate a 150-inch (3.8m) optical telescope on Siding Spring Mountain, 16 miles west of Coonabarabran, N.S. W. , where the Australian National University has an established observatory. To direct the construction and subsequent operation of the new telescope it was decided to create a statutory authority to be known as the Anglo-Australian Telescope Board. Pending the formation of this board, the two Governments, acting through the Department of Education and Science in Canberra and the Science Research Council in London, set up a joint policy committee to initiate the project.
A long non-coding RNA lnc210 promotes adipogenic differentiation of buffalo intramuscular adipocytes

Abstract Intramuscular fat (IMF) content is one of the most significant factors influencing beef quality in terms of tenderness, flavor, and juiciness. Thus, internal factors affecting IMF deposition have received considerable attention for decades. In this study, we demonstrated a long non-coding RNA, lnc210, promoted adipogenic differentiation of buffalo intramuscular adipocytes. lnc210 was rich in adipose tissue and showed increased expression with the adipogenic differentiation of buffalo intramuscular adipocytes. lnc210 was mainly expressed in the nucleus of adipocytes. Full-length lnc210 was obtained by rapid amplification of cDNA ends technology. lnc210 overexpression promoted lipid accumulation by upregulating the mRNA expression of peroxisome proliferator-activated receptor gamma (PPARG) and CCAAT enhancer binding protein alpha (C/EBPα) in buffalo intramuscular adipocytes. These results provide a basis for an in-depth analysis of the role of lnc210 in accelerating IMF deposition in buffaloes.
Incorporation of Blood and Fluid Management Within an Enhanced Recovery after Surgery Protocol in Complex Spine Surgery

Study design retrospective review. Objective Enhanced Recovery After Surgery (ERAS) is a multidisciplinary set of evidence-based interventions to reduce morbidity and accelerate postoperative recovery. Complex spine surgery carries high risks of perioperative blood loss, blood transfusion, and suboptimal fluid states. This study evaluates the efficacy of a perioperative fluid and blood management component comprised of a restrictive transfusion policy, goal directed fluid management, number of tranexamic acid (TXA) utilization, and autologous blood transfusion within our ERAS protocol for complex spine surgery. Methods A retrospective review compared patients undergoing elective complex spine surgery prior to and following implementation of an ERAS protocol with intraoperative blood and fluid management. Outcomes included incidence of blood transfusion, estimated blood loss, intraoperative crystalloids administered, frequency of intraoperative TXA utilized, incidence of patients extubated within the operating room (OR), intensive care unit (ICU) admission, and hospital length of stay. Results Following implementation, the rate of blood transfusion decreased by 11.7%(P = .017) and average crystalloid infusion was reduced 680 mL per case(P < .001). Intraoperative blood loss decreased on average 342 mL per case(P = .001) and TXA use increased significantly by 25%(P < .001). Postoperative ICU admissions declined by 8.5%(P = .071); extubation within the OR increased by 13.3%(P = .005). Conclusions This protocol presents a unique perspective with the inclusion of an interdisciplinary and comprehensive blood and fluid management protocol as an integral part of our ERAS pathway for complex spine surgery. These results indicate that a standardized approach is associated with reduced rates of blood transfusion and optimized fluid states which was correlated with decreased postoperative ICU admissions.
Microscopy Visualization of Carrier Transport in CdSeTe/CdTe Solar Cells

Solar cells are essentially minority carrier devices, and it is therefore of central importance to understand the pertinent carrier transport processes. Here, we advanced a transport imaging technique to directly visualize the charge motion and collection in the direction of relevant carrier transport and to understand the cell operation and degradation in state-of-the-art cadmium telluride solar cells. We revealed complex carrier transport profiles in the inhomogeneous polycrystalline thin-film solar cell, with the influence of electric junction, interface, recombination, and material composition. The pristine cell showed a unique dual peak in the carrier transport light intensity decay profile, and the dual peak feature disappeared on a degraded cell after light and heat stressing in the lab. The experiments, together with device modeling, suggested that selenium diffusion plays an important role in carrier transport. The work opens a new forum by which to understand the carrier transport and bridge the gap between atomic/nanometer-scale chemical/structural and submicrometer optoelectronic knowledge.
Automatic oxygen control for reducing extremes of oxygen saturation: a randomised controlled trial

Objective The objective of this study was to evaluate the efficacy of the automatic oxygen control (A-Fio2) in reducing the percentage of time spent in severe hypoxaemia (Spo2 <80%) in preterm infants for the time period on invasive ventilation and/or nasal continuous positive airway pressure (NCPAP) delivered by AVEA ventilator. Design A parallel arm randomised controlled trial. Setting A level-III neonatal intensive care unit. Patients Preterm infants (<33 weeks birth gestation) who received invasive ventilation or NCPAP in the first 72 hours of age. Interventions A-Fio2 vs manual (M-Fio2) oxygen control. Outcomes The primary outcome of the study was percentage of time spent in severe hypoxaemia (Spo2 <80%). Results 44 infants were randomised to either A-Fio2 or M-Fio2 arm and continued in the study for the period of respiratory support (invasive ventilation and/or NCPAP). The total number of study days in A-Fio2 and M-Fio2 arm were 194 and 204 days, respectively. The percentage of time spent in Spo2 <80% was significantly lower with A-Fio2 compared with M-Fio2 (median of 0.1% (IQR: 0.07–0.7) vs 0.6% (0.2–2); p=0.03). The number of prolonged episodes (>60 s) of Spo2 <80% per day was also significantly lower in A-Fio2 (0.3 (0.0–2) vs 2 (0.6–6); p=0.02). Conclusion A-Fio2 was associated with statistically significant reduction in the percentage of time spent in severe hypoxaemia when compared with M-Fio2 in preterm infants receiving respiratory support. Trial registration number NCT04223258. In this randomised controlled trial that included 44 infants, servo controlled adjustment of FiO2 improved oxygen targeting over the duration of ventilation and nasal CPAP treatment.
Airway management in penetrating thoracic trauma

Penetrating thoracic trauma accounts for 20–25% of all deaths due to trauma in the first four decades of life. About 33% of deaths from thoracic trauma occur due to penetrating trauma. In an autopsy study that enrolled 1178 trauma patients, 82% of the patients with tracheobronchial injuries died at the incidence site. In another study, 30% of those who could be transferred to the hospital died. This review aimed to revisit penetrating thoracic trauma with respect to complications and the strategies for airway management. While the risk of death in injuries with a sharp object is normally 1–8%, it reaches 25–28% when the cardiac box is included, and still, most of the patients are lost before they can come to the hospital. The consequences and management of penetrating thoracic trauma are mainly dependent on the extent of the injury to internal organs, as well as on the skill of the clinicians, airway obstruction, respiratory failure, and bleeding. Chest computed tomography (CT) is better than chest radiography in diagnosing the main bronchus or lobe/segment rupture. However, with the use of multi-channel multi-detector CT, the sensitivity of CT imaging has increased to 94% in the diagnosis of tracheobronchial injuries. While standard orotracheal intubation is sufficient in 75% of the patients, flexible bronchoscopy, intubation through the open wound or tracheostomy is required for airway provision in the rest. Clinical suspicion is the first diagnostic tool in a patient with penetrating airway trauma, and early treatment with multidisciplinary teamwork is life-saving.
Investigation of Mandarin Word Production in Children and Adults: Evidence from Phonological Priming with Non-words

Using a cross-modal picture-word interference (PWI) task, we examined phonological representations and encoding in Mandarin-speaking children and adults. Pictures of monosyllabic words were presented visually, with auditory primes presented before, concurrent with, or after the picture’s appearance (SOA -200, -100, 0, +150). Primes were related to the targets in terms of Onset, Rhyme, Tone, Onset and Tone, Rhyme and Tone, or were unrelated. The rhymes of target words were counterbalanced between simple and complex structures to examine effects of rhyme complexity. Twenty Mandarin-speaking adults (aged 20;3 to 23;10), 20 school-age children (aged 9;1 to 10;11) and 20 preschoolers (aged 5;0 to 5;11) were asked to name the pictures as quickly as possible while ignoring the primes played over a headset. The results showed that adults exhibited consistent Onset and Onset-Tone priming effects across later SOAs, while the older children (9- to 10-year-olds) exhibited Onset, Rhyme, Onset-Tone and Rhyme-Tone priming effects across later SOAs. The younger children (5-year-olds), in contrast, exhibited Rhyme and Rhyme-Tone priming effects at the earliest SOA. For both groups of children, Rhyme and Rhyme-Tone priming effects were complexity-dependent. Our findings suggest that the phonological representations of Mandarin speakers develop from holistic units into those with an onset-based structure. Moreover, an incremental processing pattern at the sub-syllabic level is gradually developed around the age of 9 or 10, though susceptibility to holistic phonological similarity is retained to some degree.