Miracle-dz/UniFAR-FTUs huggingface dataset:
Nanofibrous disc-like angle ply structures for total disc replacement in a small animal model

Low back pain affects 85% of the population and carries a socioeconomic price tag of $100 billion USD per year. Lumbar intervertebral disc disease is strongly implicated as a causative factor in back pain, as degeneration, which is ubiquitous in the population, leads to loss of normal spine function. For these reasons, our lab has developed disc-like angle ply structures (DAPS) for total disc replacement. These cell-seeded replacements are designed to match the natural hierarchical structure and function of the native disc and correct spinal kinematics after end-stage disc disease. In this dissertation, I describe the development of a rat caudal spine (tail) model of total disc replacement as a platform to evaluate DAPS in vivo; an external fixation system that immobilized caudal vertebrae at the site of implantation was required for DAPS retention and a radiopaque scaffold was developed to confirm intervertebral DAPS positioning. A detailed analysis of the DAPS in vitro growth trajectory was performed to select the optimum pre-culture duration before implantation. Cell-seeded DAPS were subsequently implanted in the rat tail and evaluated by histological, mechanical, and MRI analyses. DAPS successfully restored the mechanical properties of the native motion segment in compression, providing the first evidence of the efficacy of engineered disc replacements. Adaptations of the implant to the in vivo environment were identified; there was a reduction in glycosaminoglycan after implantation, structural modifications to the NP material, and no evidence of vertebral integration. In tackling the first of these issues, a pre-culture strategy that primed DAPS for the in vivo environment was developed; using a rat subcutaneous model, implant phenotype was best conserved post-implantation using a pre-culture strategy with a transient high dose of TGF-b3. Future work will address maintenance of NP structure, vertebral integration and scaling up to human sizes. In my work, the most promising finding was that DAPS replicated compressive motion segment mechanical properties after implantation supporting the idea that engineered biological disc replacement is a possibility for clinical treatment of advanced disc disease. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Mechanical Engineering & Applied Mechanics First Advisor Robert L. Mauck
Hyphopichia xiaguanensis f.a., sp. nov., an ascomycetous yeast species isolated from plant leaves.

Two strains representing a novel yeast species were isolated from plant leaves collected in the Baotianman Nature Reserve in Henan Province, central China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel species belonged to the genus Hyphopichia, although the formation of ascospores was not observed. The novel species was related most closely to Hyphopichia paragotoi CBS 13913T but they differed by 0.9 % sequence divergence (five substitutions) in the D1/D2 domain and by 3.7 % sequence divergence (seven substitutions and eight gaps) in the ITS region. Furthermore, the novel species can also be differentiated from the closely related species in some biochemical and physiological characteristics. The species name of Hyphopichia xiaguanensis f.a. , sp. nov. (Holotype CBS 16668, Mycobank MB 842425) is proposed to accommodate strains NYNU 20899T and NYNU 20914.
Novel role of Dipterocarpus tuberculatus as a stimulator of focal cell adhesion through the regulation of MLC2/FAK/Akt signaling pathway

ABSTRACT To investigate a novel function of Dipterocarpus tuberculatus on focal cell adhesion stimulation, alterations to the regulation of focal cell adhesion-related factors were analyzed in NHDF cells and a calvarial defect rat model after treatment with methanol extracts of D. tuberculatus (MED). MED contained gallic acid, caffeic acid, ellagic acid, and naringenin in high concentrations. The proliferation activity, focal cell adhesion ability, adhesion receptors-mediated signaling pathway in NHDF cells were increased by MED. Also, a dense adhered tissue layer and adherent cells on MED-coated titanium plate (MEDTiP) surfaces were detected during regeneration of calvarial bone. The results of the present study provide novel evidence that MED may stimulate focal cell adhesion in NHDF cells and a calvarial defect rat model.
Roles of ubiquitination in the crosstalk between tumors and the tumor microenvironment (Review)

The interaction between a tumor and the tumor microenvironment (TME) plays a key role in tumorigenesis and tumor progression. Ubiquitination, a crucial post-translational modification for regulating protein degradation and turnover, plays a role in regulating the crosstalk between a tumor and the TME. Thus, identifying the roles of ubiquitination in the process may assist researchers to investigate the mechanisms underlying tumorigenesis and tumor progression. In the present review article, new insights into the substrates for ubiquitination that are involved in the regulation of hypoxic environments, angiogenesis, chronic inflammation-mediated tumor formation, and the function of cancer-associated fibroblasts and infiltrating immune cells (tumor-associated macrophages, T-cells, myeloid-derived suppressor cells, dendritic cells, and natural killer cells) are summarized. In addition, the potential targets of the ubiquitination proteasome system within the TME for cancer therapy and their therapeutic effects are reviewed and discussed.
Damping Power System Oscillations by VSC-Based HVDC Networks: A North Sea Grid Case Study

This paper introduces a control strategy for damping of onshore power system oscillations through a voltage source converter based HVDC offshore grid. Time domain simulations are performed for a North Sea grid case study where the DC grid connects two asynchronous power systems as well as offshore wind power plants. Dynamic equivalents of the Nordic and the UK power systems are used. Simulation results demonstrate the capability of the VSC-based multi-terminal DC grid to improve onshore power system small signal stability. However, the results reveal undesired interactions between different converters in the DC grid when some are implementing power oscillation damping. This paper proposes a coordinated control scheme between onshore voltage source converters and the offshore power plant modules (PPM), to compensate for these unwanted disturbances by making use of the available energy from offshore wind PPMs.
Autophagy Protects Ocular Surface Against Overactivated Inflammation by Degrading Retinoic Acid-Induced Gene-I in Human Conjunctival Epithelial Cells.

Purpose: To evaluate the pathological role of autophagy in dry eye diseases by detecting the autophagic degradation of RIG-I, a master RNA-sensing receptor in cells. Methods: RNA-sequencing analysis and qPCR analysis of the expression level of genes related to IFN-I signaling pathway was used to evaluate the inflammatory level of cells overexpressed with RIG-I or empty vector, which was further confirmed by WB analysis. Chemical treatment (3-methyladenine, chloroquine, NH4Cl, rapamycin, torin 1 or trehalose) or gene knockdown was used to modulate autophagy. When the autophagy level was regulated, the autophagic degradation of RIG-I and its pathological role in dry eye diseases were determined by detecting the protein level of RIG-I and the level of cell inflammation. Results: Cells that overexpressed RIG-I showed increased expression of genes involved in the IFN-I signaling pathway compared with cells transfected with an empty vector. Inhibition of autophagy leaded to the accumulation of RIG-I in HCECs, combined with the aggravation of the RIG-I-mediated IFN-I signaling pathway. Contrarily, promoting the autophagic degradation of RIG-I by trehalose treatment could alleviate IFN-I signaling pathway. Conclusions: Autophagy could protect the ocular surface against IFN-I signaling pathway by degrading RIG-I in HCECs. This process may restrict the overactivation of inflammation in the pathological development of dry eye disease.
Anti-CD20 rechallenge with ofatumumab in relapsed/refractory splenic marginal zone lymphoma: the MORE trial

Splenic marginal-zone lymphoma (SMZL) accounts for , 2% of lymphoid neoplasms. 1 Treatment is indicated for progressive and symptomatic splenomegaly and for cytopenias. 2,3 The options include splenectomy, chemotherapy, rituximab with or without chemotherapy, and more recently, targeted therapies. 4-6 Results from small cohorts suggest that the anti-CD20 monoclonal antibody (mAb) rituximab can achieve deep and durable responses in many patients (overall response rate [ORR], . 80%, complete response [CR], . 40%) as a single-agent therapy. 7-10 Nevertheless, the management of relapsed and refractory SMZL remains an unmet clinical need. Novel strategies include BTK, PI3K, and immune check-point inhibitors; antibody-drug conjugates; and chimeric antigen receptor T cells. Ofatumumab a fully humanized mAb a CD20 epitope on cells with slower release from the target, with rituximab. Less frequent and milder infusion-related reac-tions (IRRs)
Changes in white matter functional networks during wakefulness and sleep

Blood oxygenation level‐dependent (BOLD) signals in the white matter (WM) have been demonstrated to encode neural activities by showing structure‐specific temporal correlations during resting‐state and task‐specific imaging of fiber pathways with various degrees of correlations in strength and time delay. Previous neuroimaging studies have shown state‐dependent functional connectivity and regional amplitude of signal fluctuations in brain gray matter across wakefulness and nonrapid eye movement (NREM) sleep cycles. However, the functional characteristics of WM during sleep remain unknown. Using simultaneous electroencephalography and functional magnetic resonance imaging data during wakefulness and NREM sleep collected from 66 healthy participants, we constructed 10 stable WM functional networks using clustering analysis. Functional connectivity between these WM functional networks and regional amplitude of WM signal fluctuations across multiple low‐frequency bands were evaluated. In general, decreased WM functional connectivity between superficial and middle layer WM functional networks was observed from wakefulness to sleep. In addition, functional connectivity between the deep and cerebellar networks was higher during light sleep and lower during both wakefulness and deep sleep. The regional fluctuation amplitude was always higher during light sleep and lower during deep sleep. Importantly, slow‐wave activity during deep sleep negatively correlated with functional connectivity between WM functional networks but positively correlated with fluctuation strength in the WM. These observations provide direct physiological evidence that neural activities in the WM are modulated by the sleep–wake cycle. This study provided the initial mapping of functional changes in WM during sleep.
Sex differences on the relation among gross motor competence, cognition, and academic achievement in children

An association between gross motor competence (GMC) and academic achievement (AA) has been described, but the potential mechanisms behind this association are still unknown. It is not known either whether these mechanisms are similar for boys and girls. The aim of this study was to analyse whether the association between GMC and AA is mediated by executive functions (EFs), and to investigate whether this mediation differs by sex. This cross‐sectional study involved 451 children aged 8 to 10 (234 girls; mean age 9.95 ± 0.59). The Movement Assessment Battery for Children‐Second Edition (MABC‐2), NIH Toolbox, and grades in language and mathematics were used to test GMC, EFs, and AA, respectively. Multifactorial structural equation model (SEM) was used to evaluate a possible relation between variables, controlling for confounders. The differences by sex were examined using a multi‐group SEM approach. The results showed that EFs acted as a full mediator of the relationship between GMC and AA in boys (β = 0.14, p = 0.012) but not in girls (β = 0.10, p = 0.326). These results show that the benefit of GMC on AA is mediated by EFs in boys but not in girls. Nevertheless, these conclusions should be carefully considered due to the cross‐sectional nature of the study.
Comparative Efficacy of Topical Ophthalmic Ganciclovir and Oral Famciclovir in Cats with Experimental Ocular Feline Herpesvirus-1 Epithelial Infection

Purpose: To determine the comparative efficacy of ganciclovir ophthalmic gel and famciclovir oral tablets in cats with experimentally induced ocular feline herpesvirus-1 (FHV-1) epithelial infection. Methods: A randomized, placebo-controlled trial was performed using 16 nonvaccinated, specific pathogen-free cats with experimental FHV-1 infection induced by topical ocular inoculation. Cats received topical ganciclovir 0.15% ophthalmic gel (1 drop 3 times daily, n = 6 cats), oral famciclovir (90 mg/kg twice daily, n = 6), or topical artificial tear gel (1 drop 3 times daily, n = 4) for 14 days. Cats were monitored after inoculation for 30 days. Ophthalmic examinations were performed every 2 days and ocular disease scores calculated. In vivo confocal microscopy was performed, and corneal leukocyte infiltrates quantified. Ocular samples for FHV-1 quantitative polymerase chain reaction (qPCR) and virus isolation assays were collected every 3 days. Hemograms and serum biochemistry panels were performed at intervals. Results: Clinical ocular disease scores and corneal leukocyte infiltrates were significantly lower in the ganciclovir and famciclovir groups compared with placebo, but no significant differences were detected between the antiviral treatment groups. Ocular viral loads determined by qPCR were significantly lower in the ganciclovir group compared with the placebo group, but there were no significant differences between the other study groups. Hemograms and biochemistry panels were unremarkable. Conclusion: Topical application of ganciclovir gel 3 times daily was well-tolerated and displayed similar efficacy at reducing clinical ocular disease scores and corneal inflammation as twice daily oral famciclovir treatment in cats with experimental ocular FHV-1 infection.
The search for an optimal means of determining the minmax control parameter using sensitivity analysis

The use of computational methods for design and simulation of control systems allows for a cost-effective trial and error approach. In this work, we are concerned with the robust, real-time control of physical systems whose state space is infinitedimensional. Such systems are known as Distributed Parameter Systems (DPS). A body whose state is heterogeneous is a distributed parameter. In particular, this work focuses on DPS systems that are governed by linear Partial Differential Equations, such as the heat equation. We specifically focus on the MinMax controller, which is regarded as being a very robust controller. The mathematical formulation of the MinMax controller involves a design parameter, 6. This parameter provides a numerical measure of the robustness of the MinMax controller; hence it is very important. However, there exists no explicit formula for determining its value in advance of attempted control design. Currently, this parameter's optimal valueoptimal in the sense of robustnessis determined experimentally using an iterative process that seeks to maintain stability in the closed loop control system as well as an always positive definite result for [/ 62PU\ (i.e. [/ — 02PYL\ > 0) where / is the identity matrix, while P and n are solutions to Algebraic Riccati Equations discussed in this dissertation. This process is obviously computationally expensive. The search for a more efficient means of determining 6, including the possibility of the emergence of an explicit formula based on some mathematically rigorous criteria,
Population spatialization at building scale based on residential population index—A case study of Qingdao city

The study of population spatialization has provided important basic data for urban planning, development, environment and other issues. With the development of urbanization, urban residential buildings are getting higher and higher, and the difference between urban and rural population density is getting larger and larger. At present, most population spatial studies adopt the grid scale, and the population in buildings is evenly divided into various grids, which will lead to the neglect of the population distribution in vertical space, and the authenticity is not strong. In order to improve the accuracy of the population distribution, this paper studied the spatial distribution of population at the building scale, combined the digital surface model (DSM) and the digital elevation model (DEM) to calculate the floor of buildings, and proposed a new index based on the total floor area of residential buildings, called residential population index (RPI). RPI is directly related to the number of people a building can accommodate, so it can effectively estimate the population of both urban and rural areas even if the structure of urban and rural buildings is very different. In addition, this paper combined remote sensing monitoring data with geographic big data and adopted principal component regression (PCR) method to construct RPI prediction model to obtain building-scale population distribution data of Qingdao in 2018, providing ideas for population spatialization research. Through field sampling survey and overall assessment, the results were basically consistent with the actual residential situation. The average error with field survey samples is 14.5%. The R2 is 0.643 and the urbanization rate is 69.7%, which are all higher than WorldPop data set. Therefore, this method can reflect the specific distribution of urban resident population, enhance the heterogeneity and complexity of population distribution, and the estimated results have important reference significance for urban management, urban resource allocation, environmental protection and other fields.
Social Reactions to Substance-Involved Sexual Assault Disclosure: Does Recipient Matter?

Victims of substance-involved sexual assault often experience worse postassault outcomes than other sexual assault victims. These outcomes may be exacerbated by their heightened risk of receiving negative reactions when disclosing their assault to informal disclosure recipients, such as friends, family members, and intimate partners. To date, little research has explored the associations between informal disclosure recipient and receipt of positive and negative reactions among victims of substance-involved sexual assault. Our sample included data about 400 victims of substance-involved sexual assault (91% women; 30% minors) who visited an anonymous sexual assault hotline and discussed reactions received during their first disclosure to an informal support provider. Using logistic regressions, we examined how the type of informal recipient to whom the victim disclosed was associated with receipt of positive and negative reactions, controlling for individual and situational characteristics (e.g., victim gender, perpetrator). Negative reactions
A potential mechanism for low tolerance feedback loops in social media flagging systems

Many people use social media as a primary information source, but their questionable reliability has pushed platforms to contain misinformation via crowdsourced flagging systems. Such systems, however, assume that users are impartial arbiters of truth. This assumption might be unwarranted, as users might be influenced by their own political biases and tolerance for opposing points of view, besides considering the truth value of a news item. In this paper we simulate a scenario in which users on one side of the polarity spectrum have different tolerance levels for the opinions of the other side. We create a model based on some assumptions about online news consumption, including echo chambers, selective exposure, and confirmation bias. A consequence of such a model is that news sources on the opposite side of the intolerant users attract more flags. We extend the base model in two ways: (i) by allowing news sources to find the path of least resistance that leads to a minimization of backlash, and (ii) by allowing users to change their tolerance level in response to a perceived lower tolerance from users on the other side of the spectrum. With these extensions, in the model we see that intolerance is attractive: news sources are nudged to move their polarity to the side of the intolerant users. Such a model does not support high-tolerance regimes: these regimes are out of equilibrium and will converge towards empirically-supported low-tolerance states under the assumption of partisan but rational users.
Ambulatory and hospital care of glaucoma in Spain and associated medical costs

Abstract Objectives This study aimed to analyze the impact of glaucoma in ambulatory centers and hospitals in Spain over the past decade, in terms of incidence and medical costs. Methods Administrative data of glaucoma patients from ambulatory centers and hospitals in Spain and registered between 2011 and 2020 was obtained from a Spanish National discharge database. Medical costs obtained were based on the diagnosis-related group-based hospital payment systems, determined by the Spanish Ministry of Health. Results Data from 100,734 ambulatory care visits and 11,408 hospital admissions related to glaucoma were obtained from the database. Most of the cases were registered in patients over 65 years of age. The incidence of glaucoma in ambulatory centers was 2.2 per 10,000 persons over the study period; in hospitals, the incidence was 0.2 per 10,000 persons. The incidence of glaucoma in ambulatory settings increased significantly over the study period, while the incidence in hospitals decreased over the same period. Most ambulatory visits and hospital admissions were programmed or non-urgent. The mean cost per ambulatory visit was €954, €5.5 million in total annually; the mean cost per hospital admission was €3,727, €4.1 million in total annually. The annual cost of ambulatory care increased significantly over the study period, while the annual cost of hospital care decreased significantly. Conclusions The medical costs of glaucoma in Spanish hospitals decreased over the study period, while the costs of ambulatory care increased. This shift in the management of glaucoma should be considered in future resource allocation decisions.