Miracle-dz/UniFAR-FTUs huggingface dataset:
DETECTION OF ENTEROTOXIGENIC METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN DAIRY DESSERTS BY MULTIPLEX-PCR

Received at: 31/3/2015 Accepted: 6/5/2015 The aim of this study is to evaluate the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) in dairy desserts samples and to investigate the antimicrobial resistance and molecular characteristics of these strains using PCR. A total of 120 samples comprising sweetened whipped cream, mehallabeia, ice cream and rice with milk were randomly collected from confectioneries, dairy shops, primitive restaurants and supermarkets in Sohag city, Egypt (30 samples each) and examined for presence of S. aureus. The results revealed that S. aureus could be detected in 23.3% of sweetened whipped cream, 6.7% of mehallabeia, 16.7% of ice cream and 3.3% of rice with milk samples. The results of antibiogram testing revealed that the highest percentage 9 (7.5%) of S. aureus isolates showed a complete resistance and 4(3.3%) showed intermediate resistance. However, the lowest percentage 2(1.7%) of the isolates were sensitive to methicillin. Eight out of nine strains that showed complete resistance using antibiotic sensitivity test identified as MRSA by detection of mecA gene by PCR (five from sweetened whipped cream, one from mehallbeia and two from ice cream samples). Furthermore, some classical enterotoxins gene profile of complete resistant strain were investigated by using M-PCR. The enterotoxins were detected in four strains only, and three different toxinotypes were recorded. The most frequent ones were “sea” gene, and followed by "sed & seb" from sweetened whipped cream and ice cream samples, while no "sec" gene could be detected from all samples. It is emphasized that the presence of S. aureus and their SEs genes in dairy desserts may be regarded as a potential risk for human health.
CCIDE_A_224400 373..382

Samar A Al-Saleh 1 Deema A Al-Shammery Nada A Al-Shehri Ebtissam M Al-Madi 4 1Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Riyadh 11527, Saudi Arabia; 2Department of Preventive Dentistry, College of Dentistry, Riyadh Elm University, Riyadh, Saudi Arabia; 3Division of Orthodontics, Department of Preventive Dental Sciences, College of Dentistry, Princess Nourah Bint AbdulRahman University, Riyadh, Saudi Arabia; 4Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11527, Saudi Arabia Purpose: This study investigated the level of esthetic awareness of dental students and professionals. Material and methods: Photographs depicting facial and smile features that deviate from universally accepted esthetic standards were presented in a questionnaire. Participants were asked to rate the images and to identify the main discrepant criteria. Eight hundred questionnaires were distributed to dental students, and clinicians. The data were analyzed using chi-square tests, Student’s t-test and one-way ANOVA with Tukey’s post hoc tests for multiple comparisons. Results: Students had 45.2% correct answers compared to 51.6% for clinicians. Among students, the highest awareness was found among 5th year students, followed by interns, 4th year and 3rd year students. The difference in the percentage of correct answers between clinicians and students overall was significantly higher for some criteria than for others, such as gingival esthetics, lip features, smile zone (incisal plane), facial features, and buccal corridor. Among clinicians, specialists responded correctly more often than did general practitioners in most of the investigated aspects. Conclusion: The ability of different group samples to diagnose discrepancies of smile esthetics was refined and enhanced with increased clinical experience and knowledge.
RMHP_A_265030 1999..2010

Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran Abstract: Novel Coronavirus, also known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in December 2019 in China and spread rapidly all around the world infecting many people. To date, no specific vaccines and drugs have been developed for this disease. Also, due to the COVID-19 pandemic and high prevalence of the infected patients, the drugs and the therapies of other past viral epidemics have been used for this disease. Many studies have been performed on the specific treatments to find whether or not they are effective on COVID-19 patients. In this review, we collected information about the most widely used drugs to treat COVID-19 (coronavirus disease 2019) belonging to groups of antivirals, antibiotics, immune modulators, and anticoagulants. Some of these compounds and drugs were used directly by inpatients, so researchers have examined others in laboratory conditions. This study considered the pros and cons of using these treatments separately and together and compared their results. By studying this review, we hope to provide useful information for researchers.
Interaction of FtsZ with GTP

Mutations in the essential cell division geneftsZ confer resistance to SulA, a cell division inhibitor that is induced as part of the SOS response. In this study we have purified and characterized the gene products of six of these mutantftsZ alleles,ftsZl ,ftsZ2,ftsZ3,ftsZ9,ftsZO00, andftsZ114, and compared their properties to those of the wild-type gene product. The binding of GTP was differentially affected by these mutations. FtsZ3 exhibited no detectable GTP binding, and FtsZ9 and FtsZ1OO exhibited markedly reduced GTP binding. In contrast, FtsZl and FtsZ2 bound GTP almost as well as the wild type, and FtsZ114 displayed increased GTP binding. Furthermore, we observed that all mutant FtsZ proteins exhibited markedly reduced intrinsic GTPase activity. It is likely that mutations inftsZ that confer sulA resistance alter the conformation of the protein such that it assumes the active form.
LOGIC PROGRAMMING AND NEGATION: A SURVEY KRZYSZTOF R. APT AND ROLAND N. BOL

Nonmonotonic reasoning grew out of attempts to capture the essential aspects of common-sense reasoning. It resulted in a number of important formalisms, the most known of them being the circumscription method of McCarthy [101], the default theory of Reiter [136], and autoepistemic logic of Moore [104]. (For a systematic comparison of these approaches, see the recently published Marek and Truszczynski [98].) One of the striking features of logic programming is that it can naturally support nonmonotonic reasoning-by means of negative literals. Many concepts introduced in the area of nonmonotonic reasoning have a natural counterpart within logic programming in spite of its limited syntax. The dual interpretation of logic programs-as a computational mechanism and as a formalism for knowledge representation-provided a fertile ground for a study of proof theory and semantics of programs which support nonmonotonic reasoning. This paper attempts to survey the outcome of this research. This subject, or some fragments of it, were already discussed in no less than five previous survey articles: Shepherdson [149, 150], Przymusinska and Przymusinski [113], Bidoit [19], and Clark [37]. Moreover, while writing this paper we learned of another survey-that of Dix [46], who focuses on the nonmonotonic reasoning aspects of
Preserving Privacy with Fine-grained Authorization in an Identity Management System

—In policy-based management, service providers want to enforce ﬁne-grained policies for their resources and services. Besides the assurance of digital identity, service providers usually need personal data for evaluation of access control policies. The disclosure of personal data, also known as Personally Identiﬁable Information (PII), could represent a privacy breach. This paper proposes an architecture that allows an individual to obtain services without the need of releasing all personal attributes. The architecture achieves that outcome evaluating the targeted policy in the domain of the identity provider, that is, policies are sent from service providers to identity providers to be evaluated, without the need of releasing some PIIs to the service provider side. We also present an implementation of a prototype using XACML 3.0 for ﬁne-grained authorization and OpenID Connect for identity management. The prototype was evaluated through an use case representing an hypothetical scenario of a bookstore. The project demonstrated that for certain situations an user can restrict the release of PII data and still gain access to services.
Endoproteolytic Cleavage in the Extracellular Domain of the Integral Plasma Membrane Protein CE9 Precedes Its Redistribution from the Pbsterior to the Anterior Tail of the Rat Spermatozoon during Epididymal Maturation

Originally identified as a basolateral domain-specific integral plasma membrane protein of the rat hepatocyte, CE9 mRNA and protein were also detected at high levels in the testis of the rat by Northern and Western blotting and immunoprecipitation . CE9 proved to be a domain-specific integral plasma membrane protein of the rat spermatozoon: on testicular spermatozoa, it was concentrated within the posterior tail domain of the plasma membrane, whereas on vas deferens spermatozoa, CE9 was concentrated within the anterior tail domain. This change in the localization of CE9 E PITHELIAL cells and other polarized cells, such as neurons and spermatozoa, compartmentalize their plasma membrane proteins to distinct domains, pre sumably to cope with an asymmetrical extracellular world (reviewed by Simons and Fuller, 1985) . Previous studies have implicated tight junctions and/or the membrane skel-eton in restricting the lateral diffusion ofcertain integral plas-mamembrane proteins on epithelial cells (reviewed byRodri-guez-Boulan and Nelson, 1989). However, considerably less is known about how this compartmentalization might be achieved by other types of polarized cells. The nonepithelial remodels
The Sharer

TO THE EDITOR: I wish to commend you on the publication of Dr. Lorenz's essay (1), which describes the author's experience with a Marine sergeant during training at a military base. The subject is portrayed as a man of courage, dignity, and honor, yet not without moral ambiguity related to past actions while he was in combat. As an attending physician in a university teaching program, I have been disturbed by the lack of respect shown toward veterans. Derogatory comments are freely expressed, the nature of which could cause considerable difficulty for the purveyor if directed toward a racial or gender category. Why are such attitudes toward veterans accepted in an atmosphere otherwise intolerant of prejudicial statements? The prestige and importance of the military remain in eclipse as those who came of age during the sixties assume positions of leadership and power. Current medicine trainees, offspring of the latter, might well reflect these same attitudes of alienation and disregard for defense-related issues. Most professionals do not partake in military service. The citizen soldier (2) is probably a relic of the past. I do find it difficult to understand Dr. Lorenz's identification of the darker aspects of his training experience with the horrors of combat as purveyed by the Gunnery Sergeant. It is harder to reconcile the fact that so many have paid the price of a shattered or lost life so that the rest of us may remain secure, well-compensated, and free to indulge in the self-absorbed angst that seems to characterize our profession, from the training period on. I think of this every day passing the poor souls smoking outside of the New England Veterans Homeless Shelter. It is imperative that we never lose sight of these sacrifices. Dr. Lorenz's contribution is a good starting point.
Fahy, Samantha and Cooper, Sue and Coleman, Tim and Naughton, Felix and Bauld, Linda (2014) Provision of smoking cessation support for pregnant women in England: results from an online survey of NHS stop smoking services for pregnant women. BMC Health

Background: Smoking during pregnancy is a major public health concern and an NHS priority. In 2010, 26% of UK women smoked immediately before or during their pregnancy and 12% smoked continuously. Smoking cessation support is provided through free at the point of use Stop Smoking Services for Pregnant women (SSSP). However, to date, little is known of how these services provide support across England. The aim of this study was to describe the key elements of support provided through English SSSP. Methods: SSSP managers were invited to participate in this survey by email. Data were then collected via an online questionnaire; one survey was completed for each SSSP. Up to four reminder emails were sent over a two month period. Results: 86% (121 of 141) of services completed the survey. Responding services were, on average, larger than non-responding services in terms of the number of pregnant women setting quit dates and successfully quitting (p < 0.01). In line with the 2010 NICE guidelines, Stop Smoking in Pregnancy and following Childbirth, one in five SSSP identified pregnant smokers using carbon monoxide (CO) testing and refer via an opt-out pathway. All services offered nicotine replacement therapy (NRT) to pregnant women and 87% of services also offered dual therapy NRT, i.e. combination of a patch and short acting NRT product. The 2010 NICE guidelines note that services should be flexible and client-centred. Consistent with this, SSSP offer pregnant women a range of support types (median 4) including couple/family, group (open or closed) or one-to-one. These are available in a number of locations (median 5), including in community venues, clinics and women’s homes. Conclusions: English Stop Smoking Services offer behavioural support and pharmacotherapy to pregnant women motivated to quit smoking. Interventions provided are generally evidence-based and delivered in a variety of both social and health care settings.
Pluralistic Coordination

Two questions are examined. Why is coordination hard to achieve when teams are diverse? Are there conditions under which players of MMOGs can learn skills of effective coordination and transfer these skills to real teams? A pluralistic network is a social system in which people are committed to working together effectively despite cultural differences. A core set of eight practices enables a network to be pluralistic. An experiment with the World of Warcraft game confirmed that the game can significantly accelerate learning of those practices. To enable the skills to be transferred to the real world, the game must be augmented with a reflective learning environment
Concrete Crack Detection Using the Integration of Convolutional Neural Network and Support Vector Machine

Crack detection in concrete structures is an important task in the inspection of buildings to ensure their safety and serviceability. Previous studies relating to crack detection via image-processing and machine learning techniques generally involve the complex modelling of cracks and the extraction of hand crafted crack features. This approach often fails to apply to images from a real environment. This paper proposes a new image-based crack detection system using a combined model of classifiers, namely a Convolutional Neural Network (CNN) and a Support Vector Machine (SVM), which was proven to perform better than the methods involving the handcrafted features. In the proposed technique, a CNN is used in extracting deep convolutional features from the RGB images of cracks and an SVM classifier is used as an alternative to a softmax layer to enhance classification ability. The combined model automatically extracts features and determines whether or not an image patch belongs to a crack class. A dataset of 550 images are collected by a digital camera from various locations, and from the results, it is concluded that the proposed method is able to identify cracks on concrete surface with an accuracy of 90.76 %
Simulation Design of Chebyshev Analog Bandpass Filter for Human Sound Frequency using LTspice Software

—Filter is a very important circuit in the signal processing system. The latest technology in the field of telecommunications must have a series of filters on the system. Filters are also often found in telecommunications systems that use human voice as a source of information such as mobile phones. When recording human voices using a cell phone, the recorded sound is often mixed with noise because it is used in noisy environments, and the information recorded becomes unclear. This problem can be overcome by installing a filter that can pass the frequency range of human voice that is between 300 Hz to 3400 Hz, and in this experiment we are using an analog chebyshev bandpass filter. In this study, it was found that to pass a frequency bandwidth of 300 Hz to 3400 Hz required an order of 22 bandpass orders by arranging in series of 11 Delyannis and Friends bandpass filter circuits. Filter simulations designed in this study by using LTspice show that the filter is able to pass signals in the frequency range 300 Hz to 3400 Hz, and damped signals that have frequencies under the 300 Hz limit and above 3400 Hz according to the desired filter specifications.
HLA-DR2 subtypes form an additional supertypic family of DRJ3 alleles

Homozygous lymphoblastoid cell lines representing Dw subtypes of the DR2 serotype were studied for structural polymorphism at DR(3. These subtypes included Dw2, Dw12, and non-Dw2/non-Dwl2. Analysis by two-dimensional gel electrophoresis showed that two DRP genes were expressed in each cell line studied. One of these expressed genes encoded a protein that was nonpolymorphic on two-dimensional gel electrophoresis among all subtypes. The second expressed DR(3 gene was polymorphic and migrated to a position on two-dimensional gels dependent on the subtype of the cell line. cDNA sequence analysis ofthe DRI3 genes revealed a DR8 gene identical between the Dw2 and Dw12 subtypes, which correlates with the nonpolymorphic spot on two-dimensional gels. A second gene was sequenced that exhibited variability between the Dw2 and Dw12 subtypes. This variability takes the form of clustered point mutations in the first domain of the molecules. The alleles from the DR(3 genes of a non-Dw2/non-Dwl2 cell line, AZH, were unusual in sequence. In contrast to other DR(3 alleles, the AZH genes may have been generated by a double recombinational event between two DRp loci from a DR2 parent. The DR2 serotype may also constitute a supertypic "family," with one DR13 gene relatively
SUSTAINABILITY AND VALUATION OF ECOSYSTEM SERVICES

The planet's population is totally dependent on its ecosystems and ecosystem services offered. The planet's capacity to support its diver sity of species, including humans, is large, but essentially limited. In this context, the envir onmental issue has been often discussed from a strategy based on the concept of ecosystem servic es and their valuation must be understood as an essential tool in organizing information, add ressing both the direct process of decision making and the provision of subsidies in the formul ation of public policies that contribute to the sustainable management of environmental resourc es. This study was developed in Araras city, Sao Paulo, Brazil, which has a strong traditi on of agricultural exploitation, with the intensive use of agricultural inputs and a large ag ricultural mechanization as well as excessive deforestation that already point to the presence of serious environmental problems. This scenario may represent a situation of environmental unsustainability and put at risk the supply of ecosystem services. Thus, this work sought to an swer the following question: (i) Is the occupation of lands in Araras city done sustainably ? Methodologically, we used the concept of sustainable scale recommended by the Ecological E onomics, establishing the capacity of land use in the city and defining an environmental sustainability index with the support of Multicriteria for Decision Aid Constructivist met hod (MCDAC), making use of MMacbeth software (Bana Costa et al., 1995). We also used va luation methods of Environmental Economics to quantify and value ecosystem services of riparian forests, carbon sequestration by the soil through the roots and phytomass, loss o f urface water due to flowage and provision of ecosystem services (production). Three scenarios were defined: (1) current land use, (2) current land use with recovery of Permanen t Protection Areas 1 (PPA) and (3) land use in accordance with the APP and Legal Reserve 2 (LG) use and recovery capacity. A sustainability index was defined for each agricultu ral explored activity in the city and considering soils management and conservation crite ria, pesticides use, fertilizer use and legal observation of PPA and RL occupancy. The same metho dological procedure was applied to the three idealized scenarios. The results show an improvement in the sustainability indexes
Detection of Non-Speech Human Sounds for Surveillance

: The objective of this research is to develop feature extraction and classification techniques for the task of Acoustic Event Detection (AED) in unstructured environments, which are those where adverse effects such as noise, distortion and multiple sources are likely to occur. The objective is to design a system that can achieve human-like sound recognition performance on a range of hearing tasks in different circumstances. The research is important, as the field is commonly overshadowed by the more popular area of Automatic Speech Recognition (ASR), and typical AED systems are often based on techniques taken directly from this. However, the direct application presents difficulties, as the characteristics of acoustic events are less well defined than those of speech, and there is no sub-word dictionary available like the phonemes in speech. Therefore, it is relevant to develop a system that can accomplish well for this challenging task.
Molecular Cloning of POEM: A Novel Adhesion Molecule That Interacts with α8β1 Integrin

Cell adhesion molecules are involved in a number of biological functions, such as cell survival, cell differentiation, tissue repair, and development. A novel molecule, POEM (preosteoblast EGF-like repeat protein with MAM domain), was isolated by RT-PCR using a set of degenerate primers designed after other known epidermal growth factor (EGF)-like motifs. From its structure, POEM was suggested to be a novel adhesion molecule with 5 EGF-like domains, an RGD cell binding motif, and a MAM domain. By in situ hybridization using E16.5-day mouse embryos, strong expression of POEM mRNA was observed in developing kidney renal tubules, parathyroid and thyroid glands, developing bone, tooth germ, and endocrine organs of the brain. The inner ear, skeletal muscle, smooth muscle except vascular system, and skin were also positive for POEM expression. Bacterial recombinant POEM protein containing the RGD sequence and MAM domain showed strong cell adhesion, spreading, and survival promoting activities. By mutating the RGD sequence to RGE, the cell spreading and survival activities were significantly decreased; but the MAM domain was shown to contribute only to the cell adhesion but not to the cell spreading and survival promoting activities. The distribution of POEM in several tissues was close to that of α8β1 integrin. Therefore, we conducted cell adhesion assays using KA8 cells, a K562 leukemia clone stably expressing α8 integrin. Parental K562 cells, which expressed α5β1 integrin, bound to fibronectin but not to POEM. On the other hand, KA8 showed strong binding and spreading on both fibronectin and POEM. These results suggest that POEM is a novel ligand for α8β1 integrin and that POEM may be involved in development and function of various tissues, such as kidney, bone, muscles, and endocrine organs. 2 by gest on M arch 4, 2020 hp://w w w . jb.org/ D ow nladed from