Miracle-dz/UniFAR-FTUs huggingface dataset:
Safety and efficacy of intravenous Tirofiban infusion after mechanical thrombectomy in acute ischemic stroke: a retrospective observational study.

OBJECTIVE To investigate the safety and efficacy of intravenous Tirofiban infusion after mechanical thrombectomy in patients with acute ischemic stroke. METHODS A consecutive series of patients with acute ischemic stroke who underwent mechanical thrombectomy were included. The patients were categorized into two groups according to whether they received intravenous Tirofiban infusion after mechanical thrombectomy. Intracranial hemorrhage (ICH) and all-cause mortality were studied as safety outcomes; recanalization of target vessel evaluated by thrombolysis in cerebral infarct (TICI) scale, and neurological improvement evaluated by Institutes of Health Stroke Scale (NIHSS) and modified Rankin scale (mRS) were studied as efficacy outcomes. RESULTS A total of 31 patients who underwent mechanical thrombectomy were enrolled, among which 8 (25.81%) received a standard dose of intravenous Tirofiban infusion after mechanical thrombectomy. There was no significant difference in baseline characteristics between the two groups (all P>0.05). None (0.00%) of the patients suffered ICH in the Tirofiban group, while 3 (13.04%) suffered ICH in the control group (P=0.550); similar all-cause mortality rates were found in both groups (25.00% versus 17.39%, P=0.634). In the Tirofiban group, all patients achieved successful recanalization defined by TICI groups (25.00% versus 17.39%, P=0.634). In the und in both groups (25.00% versus 17.39%, P=0.634). In th4). In thle, and neurological improvement evaluated so, 'et al' is notned by 3-month mRS≤2, which were not statistically significant when compared to the control group (all P>0.05). CONCLUSION Intravenous Tirofiban infusion after mechanical thrombectomy is safe and effective in patients with acute ischemic stroke.
The advantages and synergistic effects of Gunnera (Gundelia tournefortii L.) extract and protexin in chicken production

Abstract Background Probiotics and phytogenics in the poultry diet have many positive effects on productivity. The combination of these feed additives has not been studied. Objective This study was designed for evaluation of synergistic effects of protexin (P) and Gunnera (Gundelia tournefortii L.) extract (GX) on growth, biochemical, hematological and antioxidant status of broiler chickens. Methods Totally, 300 chicks were divided into 4 groups that fed the basal diet, diet containing P, GX, and GX plus P (GX‐P) in all over the growing period. At 42 days of age, blood samples were collected from all chickens. The concentration of aspartate transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglyceride (TG), cholesterol (CHL), low‐density lipoprotein (LDL), high‐density lipoprotein (HDL), superoxide dismutase (SOD), total antioxidant capacity (TAC), Glutathione peroxidase (GPx), haematological parameters, and humeral antibody against Newcastle disease vaccine was measured. Results The Results showed that the feed conversion ratio in chickens fed GX‐P was significantly lower than others. Also, in chickens fed GX or GX‐P, the TG and CHL concentration was significantly lower and GPx and TAC concentration was significantly higher than others, while chickens that received P or GX‐P showed higher haemoglobin and TP concentration. The antibody response was significantly higher in chickens fed P. The ALT, AST, ALP, and SOD concentration did not show any significant difference in all chickens. Conclusion Continuous utilization of P along with GX in broiler diets can induce synergist effect on feed efficacy and antioxidant status, lowering lipid profiles with no effect on liver function in chickens.
Systematic review and trial sequential analysis of high-intensity focused ultrasound combined with chemotherapy versus chemotherapy in the treatment of unresectable pancreatic ductal adenocarcinoma

Abstract Purpose This study aimed to compare the survival benefits between high-intensity focused ultrasound (HIFU) combined with chemotherapy and chemotherapy alone in patients with unresectable pancreatic ductal adenocarcinoma (PDAC). Methods All randomized clinical trials (RCTs) and observational studies were systematically searched through the databases of PubMed, EMBASE, CNKi and CQVIP up to December 2020. Case reports, case series and nonsystematic reviews were excluded. A meta-analysis was conducted to generate combined hazard ratios (HRs) with 95% confidence intervals (CI) for overall survival (OS). Results Seven trials, containing a total of 992 patients, were included in this study. The meta-analysis showed that a combination of HIFU and chemotherapy increased overall survival compared with chemotherapy alone, with a pooled HR of 0.40 (95% confidence interval [CI], 0.28–0.58). The combined therapy group had a significant advantage in 1-year survival rate (OR: 0.35, 95% CI: 0.22–0.53, p = 0.00). The trial sequence analysis (TSA) showed that there were enough trials to control for random errors. Conclusion Our analysis suggests that HIFU combined with chemotherapy intravenously will prolong survival for unresectable PDAC patients. The TSA showed that the survival benefit of combined therapy was definitive and there was no need to expand the sample size for repetitive exploration.
Stigma Determines Antiretroviral Adherence in Adults With HIV in Myanmar

Supplemental Digital Content is Available in the Text. Introduction: Understanding social and structural barriers that determine antiretroviral therapy (ART) adherence can improve care. Assessment of such factors is limited in Myanmar, a country with high HIV prevalence and increasing number of people living with HIV initiating ART. Methods: Questionnaires were administered to adults with HIV across 4 Myanmar cities to estimate adherence and its potential determinants, including HIV knowledge, social support, barriers to care, enacted and internalized stigma, and engagement in peer-to-peer HIV counseling (PC). Associations were determined using logistic mixed-effects modeling. Results: Among 956 participants, the mean age was 39 years, 52% were female, 36% had CD4 <350 cells/mm3, and 50% received pre-ART PC. Good adherence was reported by 74% of participants who had better HIV knowledge than those reporting nonadherence. Among nonadherent, 44% were forgetful and 81% were careless about taking ART. Among all participants, most (53%) were very satisfied with their social support and 79% reported lack of financial resources as barriers to care. Participants most frequently reported being viewed differently by others (30%) and feeling as if they were paying for past karma or sins because of their HIV diagnosis (66%). Enacted stigma (odds ratio 0.86; 95% confidence interval 0.79 to 0.92, P < 0.01) and internalized stigma (odds ratio 0.73; 95% confidence interval: 0.56 to 0.95, P = 0.023) were associated with worse adherence. Conclusions: Increased self-reported ART adherence in Myanmar is associated with less enacted and internalized stigma. These findings suggest the benefit of developing and promoting adherence interventions, which are focused on mitigating HIV-related stigma in the county.
Ablation of mitochondrial DNA results in widespread remodeling of the mitochondrial complexome

So‐called ρ0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How cells adapt to survive ablation of oxidative phosphorylation remains poorly understood. Complexome profiling analysis of ρ0 cells covered 1,002 mitochondrial proteins and revealed changes in abundance and organization of numerous multiprotein complexes including previously not described assemblies. Beyond multiple subassemblies of complexes that would normally contain components encoded by mitochondrial DNA, we observed widespread reorganization of the complexome. This included distinct changes in the expression pattern of adenine nucleotide carrier isoforms, other mitochondrial transporters, and components of the protein import machinery. Remarkably, ablation of mitochondrial DNA hardly affected the complexes organizing cristae junctions indicating that the altered cristae morphology in ρ0 mitochondria predominantly resulted from the loss of complex V dimers required to impose narrow curvatures to the inner membrane. Our data provide a comprehensive resource for in‐depth analysis of remodeling of the mitochondrial complexome in response to respiratory deficiency.
Occupational radiation exposure in doctors: an analysis of exposure rates over 25 years.

OBJECTIVES Healthcare professionals' occupational exposure to ionising radiation may be increasing due to increasing use of imaging and image-guided intervention. This study aims to assess the occupational exposure of doctors over a 25-year period at an NHS teaching hospital. METHODS Dosemeter measurements were collected prospectively from 1995 to 2019. Two retrospective analyses were performed over time (first including all measurements, second excluding "zero-dose" measurements), and by speciality. Group comparisons were undertaken using multilevel linear regression; a p-value <0.05 was deemed significant. RESULTS 8,892 measurements (3,983 body, 1,514 collar, 649 eye, 2,846 hand), of which 3,350 were non-zero measurements (1,541 body, 883 collar, 155 eye, 771 hand), were included. Whole dataset analysis found a significant decrease in exposure for radiologists and cardiologists, as measured by body, hand and collar dosemeters over the last 25 years (p < 0.01 for all). The non-zero readings reflect the whole cohort analysis except in the case of eye dosemeters, which showed a significant decrease in exposure for cardiologists (p < 0.01), but a significant increase for radiologists and surgeons/anaesthetists (p < 0.01 for both). CONCLUSIONS Whilst ionising radiation remains an occupational risk for doctors, the overall decreasing trend in occupational exposure is reassuring. However, a significant rise in eye dose for radiologists, surgeons and anaesthetists is concerning, and close monitoring is required to prevent future issues. ADVANCES IN KNOWLEDGE This paper is one of few evaluating the occupational radiation exposure to doctors over a 25-year period, showing that although most dosemeter measurements reflect decreasing exposure, the increase in eye exposure warrants caution.
Evaluation of the clinical value of routine radiographic examination during convalescence for tibial plateau-leveling osteotomy.

OBJECTIVE To determine how frequently routine follow-up radiographic findings would result in a change to the postoperative plan following tibial plateau-leveling osteotomy (TPLO) in dogs. STUDY DESIGN Retrospective study SAMPLE POPULATION: Short-term group: 100 cases; intermediate-term group: 50 cases. METHODS Medical records of 100 consecutive cases meeting the inclusion criteria were reviewed (the short-term group). The cases had no owner-perceived issues and underwent routinely prescribed radiographic follow up between 40 and 60 postoperative days after TPLO performed by one experienced surgeon. Complications identified on physical examination (PE) and radiographic examination (RE) were recorded, along with any changes to the postoperative plan. Medical records of 50 consecutive cases that had short-term and intermediate-term (≥180 days) REs and PEs were reviewed similarly (intermediate-term group). RESULTS Fifty-one cases in the short-term group had no complications on PE or RE. Forty-nine dogs were diagnosed with minor complications (patellar ligament desmitis, patella or fibula fracture, gait abnormalities): 42 on RE only; 6 on PE and RE; 1 on PE only. Exercise restriction was extended for 2 weeks in 2 cases with radiographic patellar ligament desmitis. Two cases in the intermediate-term group had minor complications at intermediate-term RE. No new PE or RE complications developed between short-term and intermediate-term evaluations. CONCLUSION At routine rechecks of dogs with no owner-perceived issues after TPLO, 49% had minor complications but only 2% were deemed significant enough to alter patient management. The likelihood of new radiographic complications developing after short-term evaluation is low. CLINICAL SIGNIFICANCE Routine radiographic recheck examinations rarely altered the postoperative plan in TPLO cases with unremarkable clinical recoveries.
Frequency and Patterns of Brain Infarction in Patients With Embolic Stroke of Undetermined Source: NAVIGATE ESUS Trial

Supplemental Digital Content is available in the text. Background and Purpose: The spectrum of brain infarction in patients with embolic stroke of undetermined source (ESUS) has not been well characterized. Our objective was to define the frequency and pattern of brain infarcts detected by magnetic resonance imaging (MRI) among patients with recent ESUS participating in a clinical trial. Methods: In the NAVIGATE ESUS trial (New Approach Rivaroxaban Inhibition of Factor Xa in a Global Trial Versus ASA to Prevent Embolism in Embolic Stroke of Undetermined Source), an MRI substudy was carried out at 87 sites in 15 countries. Participants underwent an MRI using a specified protocol near randomization. Images were interpreted centrally by those unaware of clinical characteristics. Results: Among the 918 substudy cohort participants, the mean age was 67 years and 60% were men with a median (interquartile range) of 64 (26–115) days between the qualifying ischemic stroke and MRI. On MRI, 855 (93%) had recent or chronic brain infarcts that were multiple in 646 (70%) and involved multiple arterial territories in 62% (401/646). Multiple brain infarcts were present in 68% (510/755) of those without a history of stroke or transient ischemic attack before the qualifying ESUS. Prior stroke/transient ischemic attack (P<0.001), modified Rankin Scale score >0 (P<0.001), and current tobacco use (P=0.01) were associated with multiple infarcts. Topographically, large and/or cortical infarcts were present in 89% (757/855) of patients with infarcts, while in 11% (98/855) infarcts were exclusively small and subcortical. Among those with multiple large and/or cortical infarcts, 57% (251/437) had one or more involving a different vascular territory from the qualifying ESUS. Conclusions: Most patients with ESUS, including those without prior clinical stroke or transient ischemic attack, had multiple large and/or cortical brain infarcts detected by MRI, reflecting a substantial burden of clinical stroke and covert brain infarction. Infarcts most frequently involved multiple vascular territories. Registration: URL: https://www.clinicaltrials. gov; Unique identifier: NCT02313909.
A one touch clamp and rack smart pump reduces mobilisation time and perceived burden of clinical staff members in charge of acute phase rehabilitation

Abstract The purpose of this study was to experimentally assess the effect of a one touch clamp and rack smart pump on the time needed for patient mobilisation and the clinical staff`s perceived burden associated with these procedures when compared with the traditional type infusion system. Participants were randomly assigned to one of three groups: Multi-layer rack group (MULTI), One-touch pole clamp group (ONE), and a Control group (CON). In CON, traditional type pumps were replaced from pole stands to pole stands. In MULTI, the smart pumps were replaced from multi-layer rack to multi-layer rack, and in ONE the smart pumps were replaced from pole stands to multi-layer rack. In each group, the subject round-transported the simulated patient and re-replaced the pumps. The time of both pump replacements and the transport time were significantly shorter in MULTI and ONE compared with CON (p < 0.0001). The perceived burden of the replacement and transport were all significantly lower in MULTI and ONE than CON (p < 0.0001). The one touch clamp and rack smart pump reduced the time needed and perceived burden of mobilisation. These findings are anticipated to translate into progression in the delivery of early mobilisation and ultimately rehabilitation outcomes.
Incorporation of electroendocytosis and nanosecond pulsed electric field in electrochemotherapy of breast cancer cells

ABSTRACT The study aimed to evaluate the possibility to perform electrochemotherapy using nanosecond pulsed electric field (nsPEF) and low electric field (LEF) monopolar electrical impulses to alleviate the problems of conventional electroporation. Two types of pulses have been used to treat MCF-7 human breast carcinoma cell line: very low voltage (electric field strength) long trains of short unipolar electric pulses, and low frequencies of extremely intense (40kV/cm), ultra-short (10ns) electric pulses. The electropermeabilization efficiency of the formed endocytotic vesicles was measured using the cloning efficacy test. The cell viability was decreased significantly at a repetition frequency begins from 0.01 Hz by ~35% and reached complete cell loss at 1 Hz of nanosecond pulses for cells treated before with monopolar pulses at 20 V/cm in the presence of BLM with 4 µM concentration. The uptake of non-permeant drugs has been done without plasma membrane permeabilization (classical electroporation), but by endocytosis. Nanosecond electric pulses can disrupt the membrane of endocytotic vesicles and release the cytotoxic drug bleomycin.
Study on the related risk factors and targeted nursing effects in multi-drug resistant bacteria infections in elderly patients with stroke-associated pneumonia.

PURPOSE to explore the related risk factors and targeted nursing effects in multi-drug resistant bacteria (MDRB) infection in elderly patients with stroke-associated pneumonia. METHODS We selected 100 elderly patients with MDRB who received SAP in the hospital encephalology department from May 2011 to November 2014, and pathogenic detection and drug susceptibility tests were performed on the specimens sent for inspection. In addition, we observed the pathogenic characteristics of MDRB, and carried out univariate and multivariate regression analysis of related risk factors. The infected patients were randomly divided into a control group and an intervention group, each with 50 cases. The control group received routine nursing care, and the intervention group received targeted nursing interventions, and the nursing effect of the two groups of patients was compared and analyzed. RESULTS A total of 112 drug-resistant bacteria were isolated from 100 SAP patients; gram-positive bacteria accounted for 22.3%, gram-negative bacteria accounted for 74.1%, fungi accounted for 3.6%. The multi-factor analysis showed that SAP type, ICU admission, gastric catheter retention, length of hospital stay, prophylactic use of antibiotics and disturbance of consciousness are risk factors associated with MDRB. The total effective rate of the control group was 50.0%, and the total effective rate of the intervention group was 74.0%, the difference between the two groups was statistically significant (P<0.05). CONCLUSION The pathogens causing MDRB in elderly SAP patients are mainly gram-negative bacteria, and there are many risk factors for developing MDRB in SAP patients, and targeted nursing measures can significantly improve the prognosis of patients with MDRB infection.
Structure, Mechanical and Electrochemical Properties of Thermally Reduced Graphene Oxide-poly (Vinyl Alcohol) Foams

Graphene oxide foams with a wide range of poly (vinyl alcohol) contents were synthesized by freeze casting, and then thermally reduced at 300oC in argon atmosphere. Their thermal stability, microstructure, composition and chemical states of constituents, mechanical and electrical properties were investigated by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry, compressive testing and electrochemical analysis. The results indicated that the PVA content highly influenced the crystallinity and microstructure, resulting in different mechanical properties. After thermal reduction, not only graphene oxide was reduced to graphene, but also PVA was subjected to partial pyrolysis. With the increase of the PVA content, the intensity of the sp2 C-C bond decreased while the sp3 C-C bond increased. Although the mechanical properties decreased after thermal reduction, the composite foams still showed high cyclic structure stability up to 18 % compression strain. Meanwhile, the reduced foams exhibited high electrical conductivity. Applying as anodes in lithium ion battery, the initial discharge capacity for the foams can reach 1822 mA h g-1 and it remained more than 330 mA h g-1 after 50 cycles.
Study on the value of molecular biology combined with liquid MGIT culture method in clinical examination of mycobacterium tuberculosis.

OBJECTIVE To investigate the clinical value of molecular biology (xpert MTB/RIF) combined with a liquid mycobacterium culture tube (MGIT) in the clinical examination of Mycobacterium tuberculosis (MTB). METHODS A total of 782 patients with suspected pulmonary tuberculosis who came to our hospital from February 2018 to February 2020 were selected as the research subjects. Sputum samples of all patients were taken in the morning, and BACTEC MGIT culture and xpert MTB/RIF, Lowenstein-Jenden (L-J) culture and acid fast staining microscopy were used respectively to detect the sputum samples. The positive samples were cultured, and the bacteria identification and liquid drug sensitivity test were carried out. We also analyzed the value of xpert MTB/RIF combined with MGIT culture method in the clinical testing of MTB. RESULTS (1) Among the 782 suspected patients, 405 cases were diagnosed with pulmonary tuberculosis and 377 cases were non tuberculosis patients. The sensitivity of smear microscopy was 17.28% (70/405), the specificity was 98.94% (373/377), and the average time was 3.05 h. The sensitivity of L-J culture test was 20.49% (83/405), the specificity was 97.61% (368/377), and the average time was 28.58 h. The sensitivity of MGIT culture test was 38.02% (154/405), the specificity was 96.82% (365/377), and the average time was 11.23 h. The sensitivity of xpert MTB/RIF test was 36.54% (148/405), the specificity was 99.46% (375/377), and the average time was 2.03 h. The sensitivity of MTB/RIF + MGIT test was 42.47% (172/405), the specificity was 96.02% (362/377), and the average time was 11.29 h. The sensitivity of MTB/RIF combined with MGIT culture was significantly higher than that of smear microscopy and L-J culture (χ2=61.31, 45.33, P<0.001). (2) The average culture time of 13 smear negative xpert MTB/RIF negative specimens was 17.02 days. The average culture time of 82 smear negative xpert MTB/RIF positive samples was 14.12 days. The average culture time of 77 smear positive xpert MTB/RIF positive samples was 7.45 days. (3) The sensitivity of Xpert MTB/RIF test for rifampicin resistance is 100% (11/11), and the specificity is 91.14% (144/158). CONCLUSION The method of molecular biology combined with MGIT has a high sensitivity and specificity for clinical diagnosis of MTB.