Identification of Novel Stat92E Target Genes in Drosophila Hematopoiesis

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A. 101, 14192–14197. 186 Appendix A: Fly lines Used in Genetic Interaction Assay to Identify Co-repressors and Repressors Fly lines y v hop y v hop; CgGAL4/CyO,y+ y w; CgGAL4/CyO,y+ Fly lines Type of allele RNAi of Gene BDSC 8052: w; Df(3L)ED4238 P{w+mc FRT ED4238}/TM6C Deficiency chromosome Ptp61F w; Df(3L)ED4238/TM6B Deficiency chromosome Ptp61F w*/+; CtBP/TM6B amorphic allele CtBP BDSC 19904: y w; P{EPgy2}CtBP overexpression (UAS) construct CtBP BDSC 31334: y v; P{TRiP.JF01291}attP2/TM3, Ser RNAi construct CtBP BDSC2124: gro/TM3, Sb' ethylmethanesulfonate gro w/+; gro/TM6B, Tb, Hu ethylmethanesulfonate gro y v hop; gro/TM6B ethylmethanesulfonate gro BDRC 20592: y w/ + w; gro/TM6B Tb Hu P-element gro y v hopTum-l; gro/TM6B P-element gro BDSC 511: w/+; gro'/TM6B hypomorphic allele gro y v hop; gro'/TM6B hypomorphic allele gro BDSC 417: Su(H')/In(2L)Cy, In(2R)Cy, Cy', pr' amorphic allele Su(H) y v hopTum-l; Su(H')/CyO,y+ amorphic allele Su(H) y v; P{TRiP.HM05110}attP2/TM3, Sb1 RNAi construct Su(H) y v hop; cn' bw' Kr'/CyO, y+ amorphic allele Kr BDSC11538: ry P{ry} Srp/ TM3, ryRK, Sb', Ser' amorphic allele Srp y w; ry P{ry} Srp/ TM6C Sb' Tb' amorphic allele Srp BDSC 25127: Adh n, sna', cn', vg'/ CyO amorphic allele Sna y w; Adh n7, sna', cn', vg'/ CyO, y+ amorphic allele Sna BDSC 14953: y' P{yw=SUP} amorphic allele brk 187 brk/FM7C y w hop brk /FM7C amorphic allele brk y w; dl' cn' sca'/ CyO,y+ amorphic allele dl BDSC 11753: cn' P{Pz} ken'/ CyO ry506 amorphic allele Ken y v hopTum-l; cn' ken'/CyO, y+ amorphic allele Ken y v hopTum-l; cn' ken/CyO, y+ amorphic allele Ken y w; cn' ken/CyO, y+ amorphic allele Ken VDRC 100002: P{KK102890}VIE-260B RNAi construct N y w; P{KK102890}VIE-260B RNAi construct N 188 Appendix B: Fly lines Used in Knockdown Assay Fly lines y w; CgGAL4,UAS-dcr2/CyO y+ y v hop; CgGAL4,UAS-dcr2/CyO y+ w, UASdcr2, HeGAL4 (homozygous on 3) y v hop; UASdcr2, HeGAL4/TM6 Sb' Tb' Fly lines Type of allele RNAi of Gene BDSC 33358: y' sc* v'; P{TRiP.HMS00230}attP2 RNAi construct IntS14 BDSC 31571: y' v'; P{TRiP.JF01144}attP2 RNAi construct Tango 14 BDSC 33010:y' sc* v'; P{TRiP.HMS00810}attP2 RNAi construct Capt BDSC 35036: y' sc* v'; P{TRiP.HMS01450}attP2 RNAi construct Socs36E BDSC 7886: Df(2R)Exel7142 Deficiency chromosome CG30100 BDSC 7886: Df(2R)Exel7142 Deficiency chromosome CG7747 BDSC 34901: y' sc* v'; P{TRiP.HMS01246}attP2 RNAi construct Atg9 BDSC 7567: Df(3L)Exel6088 Deficiency chromosome JTBR BDSC 35747:y' sc* v'; P{TRiP.HMS01489}attP2 RNAi construct Patj BDSC -35253: y' sc* v'; P{TRiP.GL00141}attP2 RNAi construct Ire1 BDSC4160: msi'/TM3, Sb1 amorphic allele Msi BDSC -32362: y' sc* v'; P{TRiP.HMS00353}attP2 RNAi construct Roc1a BDSC 33982: y' sc* v'; P{TRiP.HMS00942}attP2 RNAi construct Su(S) BDSC 34893: y' v'; P{TRiP HMS01238} RNAi construct Sec22 VDRC v12677: y' v'; P{TRiP GD4180} RNAi construct Rad1 VDRC v101711: y' v'; P{TRiP KK105529} RNAi construct CG15399 BDSC -31934: y' v'; P{TRiP JF02225} RNAi construct Snoo BDSC 34076: y' v'; P{TRiP HMS00575} RNAi construct Utx VDRC v107563: y' v'; P{TRiP KK106312} RNAi construct Nclb VDRC v35890: y' v'; P{TRiP GD13896} RNAi construct 128up BDSC 31344: y' v'; P{TRiP JF01302} RNAi construct HmgD VDRC v102008: y' v'; P{TRiP KK109106} RNAi construct CG14997