Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrated Analysis of miRNA and mRNA Expression Profiles Reveals the Molecular Mechanism of Posttraumatic Stress Disorder and Therapeutic Drugs

Purpose Post-traumatic stress disorder (PTSD) is a result of trauma exposure and is related to psychological suffering as a long-lasting health issue. Further analysis of the networks and genes involved in PTSD are critical to the molecular mechanisms of PTSD. Methods In this study, we aimed to identify key genes and molecular interaction networks involved in the pathogenesis of PTSD by integrating mRNA and miRNA data. Results By integrating three high-throughput datasets, 5606 differentially expressed genes (DEGs) were detected, including five differentially expressed miRNAs (DEmiRNAs) and 5525 differentially expressed mRNAs (DEmRNAs). Nineteen upregulated and 46 downregulated DEmRNAs were identified in both GSE64813 and GSE89866 datasets, while five upregulated DEmiRNAs were found in the GSE87768 dataset. Functional annotations of these DEmRNAs indicated that they were mainly enriched in blood coagulation, cell adhesion, platelet activation, and extracellular matrix (ECM)-receptor interaction. Integrated protein-protein and miRNA-protein interaction networks among the DEGs were established with the help of 65 nodes and 121 interactions. Finally, 286 small molecules were obtained based on the Drug-Gene Interaction database (DGIdb). Three genes, prostaglandin-endoperoxide synthase 1 (PTGS1), beta-tubulin gene (TUBB1), and cyclin-dependent kinase inhibitor 1A (CDKN1A), were the most promising targets for PTSD therapy. Additionally, the present study also provided a higher performance diagnostic model for PTSD based on 17 DEmRNAs, which was validated in two independent datasets, GSE109409 and GSE63878. Conclusion Our data provides a new molecular aspect that ECM-receptor interaction and the platelet activation process could be the potential molecular mechanism of PTSD, and the genes involved in this process may be promising therapeutic targets. A higher-performance diagnostic model for PTSD has also been identified.
The Photoperiod Stress Response in Arabidopsis thaliana Depends on Auxin Acting as an Antagonist to the Protectant Cytokinin

Fluctuating environmental conditions trigger adaptive responses in plants, which are regulated by phytohormones. During photoperiod stress caused by a prolongation of the light period, cytokinin (CK) has a protective function. Auxin often acts as an antagonist of CK in developmental processes and stress responses. Here, we investigated the regulation of the photoperiod stress response in Arabidopsis thaliana by auxin and its interaction with CK. Transcriptome analysis revealed an altered transcript abundance of numerous auxin metabolism and signaling genes after photoperiod stress treatment. The changes appeared earlier and were stronger in the photoperiod-stress-sensitive CK receptor mutant arabidopsis histidine kinase 2 (ahk2),3 compared to wild-type plants. The concentrations of indole-3-acetic acid (IAA), IAA-Glc and IAA-Asp increased in both genotypes, but the increases were more pronounced in ahk2,3. Genetic analysis revealed that the gain-of-function YUCCA 1 (YUC1) mutant, yuc1D, displayed an increased photoperiod stress sensitivity. In contrast, a loss of the auxin receptors TRANSPORT-INHIBITOR-RESISTANT 1 (TIR1), AUXIN SIGNALING F-BOX 2 (AFB2) and AFB3 in wild-type and ahk2,3 background caused a reduced photoperiod stress response. Overall, this study revealed that auxin promotes response to photoperiod stress antagonizing the protective CK.
Impact of Stack Engineering on HfOₓ/Al:HfOₓ-Based Flexible Resistive Memory Devices and Its Synaptic Characteristics

The effect of stack engineering of HfOx/ Al-doped HfOx(Al:HfOx)-based flexible resistive random access memory (ReRAM) devices including its synaptic characteristic has been investigated. The flexible Al/HfOx/Al:HfOx/HfOx/ITO/PET resistive memory device shows outstanding switching performance in terms of repeatability, statistical variation of parameters even in bending condition up to 5 mm. The switching mechanism has been explained by ohmic conduction in low resistance state (LRS) and the trap-controlled space charge limited conduction (SCLC) in high resistance state (HRS). The improved synaptic characteristics, such as long-term potentiation (LTP), long-term depression (LTD), spike-rate-dependent plasticity (SRDP), paired-pulse facilitation (PPF), and post-tetanic potentiation (PTP), are effectively mimicked with Al/HfOx/Al:HfOx/ITO/PET ReRAM, which is suitable for neuromorphic computing application.
Determinant of Compliance Perceptions among Bank Officers towards Anti-Money Laundering

The purpose of this study is to investigate the perceptions of determinants of anti-money laundering compliance among banking officers in mitigating money laundering. There are three independent variables that are employed in this study to understand and analyze the level of perceived compliance among the banking compliance officers, which are the regulatory comprehensiveness, training, and awareness. The data were collected through the distribution of questionnaires to 56 banks in Malaysia. The findings of this study show that there is a positive relationship between regulatory comprehensiveness, training, and awareness on the perceived level of compliance. This study recommends that efforts should be made by the banking institution to strengthen the anti-money laundering regulation and policy to improve the efficiency of financial institutions in Malaysia. In addition, the compliance officer’s competency should be enhanced to ensure better compliance quality.
Effects of Football Training and Match-Play on Hamstring Muscle Strength and Passive Hip and Ankle Range of Motion during the Competitive Season

Deficits in hamstring muscle strength and in hip range of motion (ROM) have been considered risk factors for hamstring muscle injuries. However, there is a lack of information on how chronic exposure to regular football training affects hamstring muscle strength and hip ROM. The aim of this study was to examine the longitudinal effect of football training and competition during a complete season on hamstring muscle strength and hip ROM in football players. A total of 26 semi-professional football players underwent measurements of isometric hamstring muscle strength and passive hip flexion/extension, and internal/external hip rotation (IR/ER) ROM during the football season (pre-season, mid-season, end-season). Compared to pre-season, hamstring muscle strength increased in the dominant (+11.1%, p = 0.002) and non-dominant (+10.5%, p = 0.014) limbs in the mid-season. Compared to mid-season, hamstring strength decreased in the dominant (−9.3%, p = 0.034) limb at end-season. Compared to the pre-season, hip extension ROM decreased in mid-season in the dominant (−31.7%, p = 0.007) and non-dominant (−44.1%, p = 0.004) limbs, and further decreased at end-season (−49.0%, p = 0.006 and −68.0%, p < 0.001) for the dominant and non-dominant limbs. Interlimb asymmetry for hip IR ROM increased by 57.8% (p < 0.002) from pre-season to mid-season. In summary, while hamstring muscle strength increased during the first half of the football season in football players, a progressive reduction in hip extension ROM was observed throughout the season. The reduced hip extension ROM suggests a reduced mobility of the hip flexors, e.g., iliopsoas, produced by the continuous practice of football. Consequently, hip-specific stretching and conditioning exercises programs should be implemented during the football season.
Super-resolution imaging with metal-induced energy transfer reveals effect of force on the actin cytoskeleton

Cells actively probe the mechanical properties of their environment and adapt their physiological state accordingly. For this fundamental task, they exert forces on their environment using their actomyosin machinery. This machinery consists of protein fibers consisting of dynamically assembling and dissembling actin units, so called actin stress fibers, on which motor proteins act to generate forces. This machinery is also used to react to external forces applied to cells. Transduction of forces from the actomyosin complexes to the extracellular space occurs via adaptive multi-protein complexes, so called focal adhesions. Focal adhesions re-structure depending on biochemical and mechanical signals. The distance of the extracellular matrix to the actomyosin fibers is determined by the structure of these complexes. Monitoring this distance reveals cellular adaptions to force. Here, we use metal induced energy transfer (MIET) to monitor the distance of the extracellular matrix to focal adhesions for understanding force transduction through focal adhesions. We manipulate the cells either with drugs influencing cellular force generation or using an atomic force microscope. We find that high forces lead to a multistep-restructuring of focal adhesions, rendering force transduction more efficient.
SPATA18 Expression Predicts Favorable Clinical Outcome in Colorectal Cancer

Dysregulation of mitochondrial quality control has been reported to be associated with cancer and degenerative diseases. SPATA18 (spermatogenesis-associated 18, also known as Mieap) encodes a p53-inducible protein that can induce lysosome-like organelles within mitochondria that eliminate oxidized mitochondrial proteins and has tumor suppressor functions in mitochondrial quality control. In the present study, 268 primary colorectal cancers (CRCs) were evaluated immunohistochemically for SPATA18 expression to assess its predictive utility and its association with cellular proliferation activity. Furthermore, the association with p53 immunoreactivity, a surrogate marker for TP53 mutation, was analyzed. Non-neoplastic colonic mucosa showed cytoplasmic SPATA18 expression. Seventy-two percent of the lesions (193/268) displayed high SPATA18 expression in the cytoplasm of CRC cells. Univariate analyses revealed significant associations between SPATA18 expression and tumor size (p < 0.0001), histological differentiation (p = 0.0017), and lymph node metastasis (p = 0.00039). The log-rank test revealed that patients with SPATA18-high CRCs had significantly better survival than SPATA18-low patients (p < 0.0001). Multivariate Cox hazards regression analysis identified tubular-forming histology (hazard ratio [HR] = 0.25), age < 70 years (HR = 0.50), and SPATA18-high (HR = 0.55) as potential favorable factors. Lymph node metastasis (HR = 1.98) and peritoneal metastasis (HR = 5.45) were cited as potential independent risk factors. Cellular proliferation activity was significantly higher in SPATA18-high tumors. However, no significant correlation was detected between SPATA18 expression and p53 immunoreactivity or KRAS/BRAF mutation status. On the basis of our observations, SPATA18 immunohistochemistry can be used in the prognostication of CRC patients.
Work with Foreign Scientific Editions as an Effective Factor in Motivating Undergraduates to Improve their Foreign Language Competency

The article is devoted to the problem of increase of the pedagogical universities undergraduates’ motivation to develop their foreign language competency. The main reasons necessitating continuous increase of the foreign language competence level have been determined. Utilizing such research methods as the focus group survey, theoretical generalization, comparison and pedagogical experiment, it has been proved that the maximum inclusion of students in foreign language activities with professional scientific information is an effective factor in increasing the professional orientation of the foreign languages learning by the future teachers. This type of work in the process of studying various academic disciplines provides a positive dynamics of the motivation components and readiness of the future teachers to work with scientific foreign-language information. It has been shown that netnographic analysis of the foreign-language scientific publications is also an effective factor in personal and professional development, socio-cultural communication, and the students’ mobility in professional and academic environment.
From Playing Field to Battleground: The United States Navy V-5 Preflight Program in World War II*

The question of whether sport is analogous to war has been frequently debated. Juxtaposition of war training onto the playing field has been theorized, but a laboratory for such ambitions seems a distant goal. Yet such a setting was etablished by the United States Navy in its preflight training program during World War II. John Griffith, editor of Athletic Journal, commissioner of the Western Athletic (Big 10) Conference, and member of the Joint Army Navy Committee on Welfare and Recreation (JANC), wrote on October 16, 1941 to the JANC executive director, proposing that the Armed Services “utilize the average American’s interest in sport as a means of aiding in the development of an efficient fighting force. “ A World War I bayonet training instructor, the bellicose Griffith prompted much discussion about the value of sports training to war. This soon led to his membership in a group created to implement that objective, the advisory committee for the Navy Aeronautic V-5 Preflight Schools. The nine man preflight committee was appointed in January, 1942 by Captain Arthur Radford, Director of Aeronautic Training. In addition to Griffith, it included athletic directors William Bingham of Harvard, Ray Eckmann of the University of Washington, Jack Meagher of Alabama Polytechnic Institute (Auburn), and L. W. St. John of the Ohio State University. Princeton physical educator Joseph Raycroft, Elmer Mitchell, Director of Intramurals at the University of Michigan, Bernie Bierman, celebrated University of Minnesota football coach, and Dean Carl Schott of the School of Physical Education at Pennsylvania State College, were also included. Through this group was planned the most doctrinaire sports project of World War II. 2
The Use of Big Data Analytics in Medical Applications

The field of Big Data Analytics does not have a linear capacity for growth. It is based on a specified structure. Big data is now most useful for data backup purposes, rather than for anything else. Big Data is a collection of data sets that are both numerous and complicated in nature, and it is becoming increasingly popular. They consist of both organized and unstructured data that is constantly changing at a rate that is inconvenient for traditional relational database systems and existing analytical tools to keep pace with. There is constantly some new information being introduced. It also contributes to the resolution of India's major concerns. It also contributes to closing the data gap. Healthcare is the preservation or advancement of health by the prevention, interpretation, and medical treatment of the disorder, ill health, abuse, and other significant physical, mental, and spiritual degeneration in the mortal body. Health care is conveyed by health professionals in the form of unified health experts, specialists, physician associates, midwives, nurses, antibiotics, pharmacy, psychology, and other health-related fields of expertise. Additionally, it has an introduction, challenging elements and concerns, Big Data Analytics in use, technical specifications, research applications, industrial applications, and future applications. This article aims to provide knowledge in the field of big data analytics and its use in the medical arena.
The Effect of 1-MCP and MAP Treatments on Shelf-life Quality of Suli Pear in Semi-underground Ventilation Storeroom

Suli pear was used to investigate the effects of 1-MCP and MAP treatments on shelf-life quality.The results showed that,1-MCP and MAP treatments could significantly decrease the rates of respiration and ethylene production,delay the loss of fruit ascorbic acid content and total soluble solids,delay ripening of the fruit,reduce the rates of fruit rot,obviously keep the fruit quality,extend shelf-life time.It had comparatively better effect with 0.5 μL·L-1 1-MCP or MAP treatment.
AN ASSESSMENT OF KEY HUMAN RESOURCE MANAGEMENT PRACTICES ON JOB SATISFACTION AMONG SENIOR STAFF OF THE UNIVERSITY OF CAPE COAST, GHANA

Human resource management (HRM) practices play crucial roles in ensuring that organizations deliver quality services. The study assessed the effect of selected key HRM practices on job satisfaction among employees of the University of Cape Coast. Using an explanatory design, a sample of 302 respondents was selected from a population of 1469 senior staff of the University through a stratified random sampling technique. The questionnaire was the main instrument used to collect the data. 292 respondents answered the questionnaires, which represented 96.68% of the responses rate. The analysis of data was done using the Statistical Product for Service Solution (SPSS). The dependent variable was job satisfaction. The independent variables were promotion, reward, training and development, and supervision. Pearson correlation was used to determine the relationships among the variables, and the standard multiple regression test was used to determine the level of significance of each determinant of job satisfaction. The results of the analysis show that the following HRM practices- promotion, reward, training and development, and supervision- have a moderate effect on job satisfaction; but supervision and reward have a significant positive effect on employees’ job satisfaction. The study contributed to knowledge in the research endeavor by demonstrating that although HRM practices influence job satisfaction among employees of the University of Cape Coast, promotion does not automatically make all employees enjoy their jobs.
Omega-3 Fatty Acid and its Protective Effect against Cancer and Cancer-related Complication

Cancer is a broad term for a group of diseases that begin when abnormal cells grow out of control in practically any organ or tissue of the body. A variety of nutrients are included in the diet of people with cancer but omega-3 polyunsaturated fatty acids are one of the most effective nutrients. Numerous clinical and epidemiological studies have shown that polyunsaturated omega-3 fatty acids play an important role in maintaining the health of people with cancer. Omega-3 polyunsaturated fatty acid, by their own antioxidant, antitumour, anticarcinogenic, and neuroprotective properties, help cancer patients to reduce their various physical complications. Fish oil contains omega-3 fatty acids mainly docosahexaenoic acid and eicosapentaenoic acid have antioxidant properties that help to increase antioxidant enzymes and remove the reactive oxygen species from cancer patient’s body and also reduce the oxidative stress. Now, omega-3 polyunsaturated fatty acid is considered as pharmaconutrient. As a pharmaconutrient, omega-3 fatty acid reduces the inflammatory response of cancer patients. The cyclooxygenase-2 expression is suppressed by the omega-3 fatty acid and reduce the growth of tumour cell. Mental wellness is a significant lifestyle aspect that the cancer patient cannot maintain. As a result, they are suffering from major depression. Omega-3 fatty acids exhibit neuroprotective activity against various brain diseases. This review summarizes that the omega-3 polyunsaturated fatty acids have an antioxidant and anticarcinogenic function that can inhibit the cancer cell growth and maintain the health status and lifestyle behaviour of the cancer patients.
The Business of Biotechnology

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O6-methylguanine DNA methyltransferase is upregulated in malignant transformation of gastric epithelial cells via its gene promoter DNA hypomethylation

BACKGROUND O6-methylguanine-DNA methyltransferase (MGMT) is a suicide enzyme that repairs the mispairing base O6-methyl-guanine induced by environmental and experimental carcinogens. It can transfer the alkyl group to a cysteine residue in its active site and became inactive. The chemical carcinogen N-nitroso compounds (NOCs) can directly bind to the DNA and induce the O6-methylguanine adducts, which is an important cause of gene mutation and tumorigenesis. However, the underlying regulatory mechanism of MGMT involved in NOCs-induced tumorigenesis, especially in the initiation phase, remains largely unclear. AIM To investigate the molecular regulatory mechanism of MGMT in NOCs-induced gastric cell malignant transformation and tumorigenesis. METHODS We established a gastric epithelial cell malignant transformation model induced by N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) or N-methyl-N-nitroso-urea (MNU) treatment. Cell proliferation, colony formation, soft agar, cell migration, and xenograft assays were used to verify the malignant phenotype. By using quantitative real-time polymerase chain reaction (qPCR) and Western blot analysis, we detected the MGMT expression in malignant transformed cells. We also confirmed the MGMT expression in early stage gastric tumor tissues by qPCR and immunohistochemistry. MGMT gene promoter DNA methylation level was analyzed by methylation-specific PCR and bisulfite sequencing PCR. The role of MGMT in cell malignant transformation was analyzed by colony formation and soft agar assays. RESULTS We observed a constant increase in MGMT mRNA and protein expression in gastric epithelial cell malignant transformation induced by MNNG or MNU treatment. Moreover, we found a reduction of MGMT gene promoter methylation level by methylation-specific PCR and bisulfite sequencing PCR in MNNG/MNU-treated cells. Inhibition of the MGMT expression by O6-benzylguanine promoted the MNNG/MNU-induced malignant phenotypes. Overexpression of MGMT partially reversed the cell malignant transformation process induced by MNNG/MNU. Clinical gastric tissue analysis showed that MGMT was upregulated in the precancerous lesions and metaplasia tissues, but downregulated in the gastric cancer tissues. CONCLUSION Our finding indicated that MGMT upregulation is induced via its DNA promoter hypomethylation. The highly expressed MGMT prevents the NOCs-induced cell malignant transformation and tumorigenesis, which suggests a potential novel approach for chemical carcinogenesis intervention by regulating aberrant epigenetic mechanisms.
Measuring Millimeter Wave Based Link Bandwidth Fluctuations During Indoor Immersive Experience

This letter presents the results of motion-tracking synchronized millimeter wave (mmWave) link bandwidth fluctuations while a user is engaged in immersive augmented/virtual reality applications. Our system, called MITRAS, supports extensive exploration of human-induced impacts on mmWave link bandwidth during immersive experience. MITRAS adopts the packet train measurement application to track link bandwidth fluctuations. Meanwhile, the user movements are tracked using an Oculus Quest 2 headset. Through investigating the impacts of human movements on link bandwidth fluctuations, we further propose a link state prediction model to shed light on higher layer protocol design for immersive applications over mmWave links.