Miracle-dz/UniFAR-FTUs huggingface dataset:
Acute Mechanical, Neuromuscular, and Metabolic Responses to Different Set Configurations in Resistance Training

Abstract Piqueras-Sanchiz, F, Cornejo-Daza, PJ, Sánchez-Valdepeñas, J, Bachero-Mena, B, Sánchez-Moreno, M, Martín-Rodríguez, S, García-García, Ó, and Pareja-Blanco, F. Acute mechanical, neuromuscular, and metabolic responses to different set configurations in resistance training. J Strength Cond Res 36(11): 2983–2991, 2022—The aim of this study was to investigate the effect of set configuration on mechanical performance, neuromuscular activity, metabolic response, and muscle contractile properties. Sixteen strength-trained men performed 2 training sessions in the squat exercise consisting of (a) 3 sets of 8 repetitions with 5 minutes rest between sets (3 × 8) and (b) 6 sets of 4 repetitions with 2 minutes rest between sets (6 × 4). Training intensity (75% one repetition maximum), total volume (24 repetitions), total rest (10 minutes), and training density were equalized between protocols. A battery of tests was performed before and after each protocol: (a) tensiomyography (TMG), (b) blood lactate and ammonia concentration, (c) countermovement jump, and (d) maximal voluntary isometric contraction in the squat exercise. Force, velocity, and power output values, along with electromyography data, were recorded for every repetition throughout each protocol. The 6 × 4 protocol resulted in greater mechanical performance (i.e., force, velocity, and power) and lower neuromuscular markers of fatigue (i.e., lower root mean square and higher median frequency) during the exercise compared with 3 × 8, particularly for the last repetitions of each set. The 3 × 8 protocol induced greater lactate and ammonia concentrations, greater reductions in jump height, and greater impairments in TMG-derived velocity of deformation after exercise than 6 × 4. Therefore, implementing lower-repetition sets with shorter and more frequent interset rest intervals attenuates impairments in mechanical performance, especially in the final repetitions of each set. These effects may be mediated by lower neuromuscular alterations, reduced metabolic stress, and better maintained muscle contractile properties.
Development of a Positive Body Image Chatbot (KIT) With Young People and Parents/Carers: Qualitative Focus Group Study

Background Body image and eating disorders represent a significant public health concern; however, many affected individuals never access appropriate treatment. Conversational agents or chatbots reflect a unique opportunity to target those affected online by providing psychoeducation and coping skills, thus filling the gap in service provision. Objective A world-first body image chatbot called “KIT” was designed. The aim of this study was to assess preliminary acceptability and feasibility via the collection of qualitative feedback from young people and parents/carers regarding the content, structure, and design of the chatbot, in accordance with an agile methodology strategy. The chatbot was developed in collaboration with Australia’s national eating disorder support organization, the Butterfly Foundation. Methods A conversation decision tree was designed that offered psychoeducational information on body image and eating disorders, as well as evidence-based coping strategies. A version of KIT was built as a research prototype to deliver these conversations. Six focus groups were conducted using online semistructured interviews to seek feedback on the KIT prototype. This included four groups of people seeking help for themselves (n=17; age 13-18 years) and two groups of parents/carers (n=8; age 46-57 years). Participants provided feedback on the cartoon chatbot character design, as well as the content, structure, and design of the chatbot webchat. Results Thematic analyses identified the following three main themes from the six focus groups: (1) chatbot character and design, (2) content presentation, and (3) flow. Overall, the participants provided positive feedback regarding KIT, with both young people and parents/carers generally providing similar reflections. The participants approved of KIT’s character and engagement. Specific suggestions were made regarding the brevity and tone to increase KIT’s interactivity. Conclusions Focus groups provided overall positive qualitative feedback regarding the content, structure, and design of the body image chatbot. Incorporating the feedback of lived experience from both individuals and parents/carers allowed the refinement of KIT in the development phase as per an iterative agile methodology. Further research is required to evaluate KIT’s efficacy.
Ertugliflozin + metformin as a treatment option for type 2 diabetes

ABSTRACT Introduction: Sodium-glucose cotransporter 2 inhibitors (SGLT2i) reduce blood glucose and glycosylated hemoglobin (HbA1c), the risk of hospitalization for heart failure (HF) and the incidence of serious adverse kidney function events (chronic kidney disease, CKD) in patients with type 2 diabetes mellitus (T2DM). Ertugliflozin is the last SGLT2i approved by Food and Drug Administration (FDA) in the USA and European Medical Agency (EMA) in Europe for the treatment of T2DM alone or in combination with other drugs. Areas covered: The authors critically discuss in this drug evaluation the safety and efficacy of the ertugliflozin + metformin combination in patients with T2DM without complications, those with CKD, or those with heart failure (HF). Furthermore, the authors discuss the results of the VERTIS CV trial (MK-8835-004), the trials NCT01986881 and NCT01986855 and other smaller studies. Expert opinion: The ertugliflozin + metformin combination is safe and effective in patients with T2DM with renal impairment, HF of any kind, or those without diabetic complications. These have practical implications that will help diabetologists, cardiologists, internists, nephrologists and general practitioners in selecting the proper combination of SGLT2i on top of metformin, if needed.
Time, time, time: see what governs the luteal phase endocrinology

Abstract Two modes of ovulation trigger are used in IVF: hCG, acting on ovarian LH receptors, and GnRH agonist, eliciting pituitary LH and FSH surges. These two modes are evaluated herein, focusing on how they serve specific time-sensitive events crucial for achieving embryo implantation and pregnancy. hCG trigger is associated with significant timing deviation from physiology. Peak progesterone is not synchronized with implantation window; progesterone level does not rise continuously to a mid-luteal peak, but rather drops from a too early peak. The luteal phase endocrinology post GnRH agonist trigger is characterized by a quick and irreversible luteolysis. Therefore, freeze all strategy is advised, if there is a risk of ovarian hyperstimulation syndrome. If fresh transfer is desired, numerous approaches for luteal phase support have been suggested. However, a thorough understanding of time-sensitive events suggests that a single 1,500 IU hCG dose, administered 48 h post oocyte retrieval, is all that is needed to fully support the luteal phase and secure best chances of achieving pregnancy.
Osmolyte enhanced aqueous two‐phase system for virus purification

Due to the high variation in viral surface properties, a platform method for virus purification is still lacking. A potential alternative to the high‐cost conventional methods is aqueous two‐phase systems (ATPSs). However, optimizing virus purification in ATPS requires a large experimental design space, and the optimized systems are generally found to operate at high ATPS component concentrations. The high concentrations capitalize on hydrophobic and electrostatic interactions to obtain high viral particle yields. This study investigated using osmolytes as driving force enhancers to reduce the high concentration of ATPS components while maintaining high yields. The partitioning behavior of porcine parvovirus (PPV), a nonenveloped mammalian virus, and human immunodeficiency virus‐like particle (HIV‐VLP), a yeast‐expressed enveloped VLP, were studied in a polyethylene glycol (PEG) 12 kDa‐citrate system. The partitioning of the virus modalities was enhanced by osmoprotectants glycine and betaine, while trimethylamine N‐oxide was ineffective for PPV. The increased partitioning to the PEG‐rich phase pertained only to viruses, resulting in high virus purification. Recoveries were 100% for infectious PPV and 92% for the HIV‐VLP, with high removal of the contaminant proteins and more than 60% DNA removal when glycine was added. The osmolyte‐induced ATPS demonstrated a versatile method for virus purification, irrespective of the expression system.
Immune control of Mycobacterium tuberculosis is dependent on both soluble TNFRp55 and soluble TNFRp75

Tuberculosis presents a global health challenge, and tumour necrosis factor (TNF) signalling is required for host immunity against Mycobacterium tuberculosis (Mtb). TNF receptor shedding, however, compromises effective immunity by reducing bioactive TNF through the formation of inactive complexes. In this study, we first compared the effect of total soluble TNF receptors using a transgenic p55ΔNS/p75−/− murine strain on host protection during a low‐dose aerosol Mtb H37Rv challenge. We report that the presence of membrane‐bound TNFRp55 alone in the absence of TNFRp75 results in superior control of a primary Mtb infection where p55ΔNS/p75−/− hyperactive dendritic cells displayed an increased capacity to induce a hyperactive Mtb‐specific CD4+ T‐cell response. p55ΔNS/p75−/− dendritic cells expressed a higher frequency of MHCII and increased MFIs for both CD86 and MHCII, while CD4+ T cells had higher expression of CD44 and IFN‐γ. Next, the relative contributions of soluble TNFRp55 and soluble TNFRp75 to host protection against either primary Mtb infection or during reactivation of latent tuberculosis were delineated by comparing the experimental outcomes of control C57BL/6 mice to transgenic p55ΔNS/p75−/−, p55ΔNS and p75−/− mouse strains. We found that soluble TNFRp55 is redundant for immune regulation during the chronic stages of a primary Mtb infection. However, TNFRp55 together with soluble TNFRp75 has a crucial role in immune regulation of reactivation of latent tuberculosis.
The Relationship Between Workload and Non-Contact Musculoskeletal Injury in College Swimmers: A Prospective Cohort Study.

CONTEXT Swimmers are known for high training loads and overuse injuries, but few studies have investigated the relationship between the two at the college level. OBJECTIVE To determine the association between workload and non-contact musculoskeletal injury in college swimmers. We hypothesized (1) higher overall workload (kilometers swum throughout the season) would be associated with increased odds of injury and (2) the ratio of the current week's (acute) workload divided by the current month's (chronic) workload (i.e., the acute:chronic workload ratio (ACWR)) would be more strongly associated with odds of injury than acute or chronic workloads alone. DESIGN Prospective cohort study. SETTING College natatorium. PATIENTS OR OTHER PARTICIPANTS Thirty-seven Division III college swimmers. MAIN OUTCOME MEASURES Logistic regression using generalized estimating equations was used to calculate odds ratios (OR) with 95% confidence intervals (CI) for injury relative to high workloads and high ACWRs. Injury rates for several ranges of workloads and ACWRs were also calculated. RESULTS Eleven participants (29.7%) sustained 12 injuries, with seven injuries occurring during the participants' winter training trip. Injury was associated with high acute workloads (OR = 27.1 [95% CI = 8.2, 89.8]) and high ACWRs (OR = 25.1 [95% CI = 7.7, 81.4]) but not high chronic workloads (OR = 2.6 [95% CI = 0.3, 20.0]) or overall workloads (OR = 1.00 [95% CI = 0.99, 1.01]). High acute workloads (>37.2 km/week) and high ACWRs (>1.56) increased injury rate from ≤1% to 15% and 14%, respectively, compared to all lower acute workloads and ACWRs. CONCLUSIONS College swimmers can tolerate high workloads spread out over the season. However, caution should be used when prescribing high acute workloads and high ACWRs (e.g., winter training trip) due to increased odds of injury.
Reconstruction of a large nasal–facial defect using an augmented temporal myocutaneous tube flap in a dog

Abstract The muzzle region of dogs contains various composite tissues, which are challenging to recreate during reconstruction. Small or moderate facial/nasal defects can be closed primarily or left for second‐intention healing. However, larger defects require the application of composite tissue or labial advancement flaps. Axial pattern flaps based on the caudal auricular artery, superficial temporal artery (STA), angularis oris artery, and other cutaneous arteries have been reported. In our case, we aim to report the reconstruction of a large composite defect of the rostral and dorsal nasal regions in a dog using an augmented, axial myocutaneous flap based on bilateral STAs. This is a clinical report on a spayed female mixed‐breed dog (age, 7 years; weight, 15 kg), in which a large‐scale nasal–facial composite tissue defect was surgically reconstructed using an axial myocutaneous flap based on bilateral STA branches. A delay technique was applied to prefabricate the flap to enrich the blood supply. New nostrils were created on a folded, rostral hard palate. As a result, the axial tube rotational flap was successfully transferred. The use of delay technique for prefabricating the tube flap optimized its size and survival. In addition to the folded rostral hard palate, the flap fully closed the defects on the face and nose. Functional and cosmetic outcomes were satisfactory, with minimal donor‐site morbidity. In, conclusion, a large‐scale nasal–facial defect in a dog was successfully reconstructed using an augmented tube pedicle flap based on the bilateral STAs, which may, thus, be used to repair very large facial‐nasal defects in dogs.
Contemporary Clinical Use of Aspirin: Mechanisms of Action, Current Concepts, Unresolved Questions, and Future Perspectives

Abstract The ability of aspirin to inhibit platelet aggregation has positioned this agent within the most frequently used drugs worldwide. The aim of this article is to review the contemporary clinical use of aspirin and also to discuss unresolved issues not yet translated into clinical practice. Results from several clinical trials have led to strong guideline recommendations for aspirin use in the acute management and secondary prevention of cardiovascular disease. On the contrary, guidelines regarding aspirin use as primary prevention of cardiovascular disease are almost conservative, supported by recent trials reporting that the bleeding risk outweighs the potential benefits in most patients. In pregnancy, aspirin has proved efficient in preventing preeclampsia and small-for-gestational-age births in women at high risk, and is hence widely recommended in clinical guidelines. Despite the vast amount of clinical data on aspirin, several unresolved questions remain. Randomized trials have reported that aspirin reduces the risk of recurrent venous thromboembolism, but the clinical relevance remains limited, because direct oral anticoagulants are more effective. Laboratory studies suggest that a twice-daily dosing regimen or evening intake may lead to more efficient platelet inhibition, and the potential clinical benefit of such strategies is currently being explored in ongoing clinical trials. Enteric-coated formulations of aspirin are frequently used, but it remains unclear if they are safer and as efficient as plain aspirin. In the future, aspirin use after percutaneous coronary interventions might not be mandatory in patients who also need anticoagulant therapy, as several trials support shorter aspirin duration strategies. On the other hand, new treatment indications for aspirin will likely arise, as there is growing evidence that aspirin may reduce the risk of colorectal cancer and other types of cancer.
Task and Informational Constraints on Search Strategies: Testing the Idea of Convergence to Tolerant Regions

Abstract The experiment reported was designed to investigate the interaction of information and force variability on the evolving search strategy, specifically testing the hypothesis of convergence to tolerant regions. Participants were required to produce proportional bimanual isometric force output over three days of practice, with no prespecified force target and where performance was more tolerant to force variability at higher forces. The duration of intermittent visual feedback was manipulated to test the effects of information and force variability on the search process and the resulting sensitivity to tolerant regions of the task space. The findings showed that just under half of the participants exploited more tolerant regions and that this was predicted by the initial force conditions. Different characterizations of the individual search patterns were also predicted by inherent force-dependent variability and initial force conditions. Visual intermittency feedback did not affect the time-dependent properties of the search process but did influence the within-trial variability. Our findings suggest that the attraction to tolerant regions needs to be considered within the interactions of the different categories of constraints on the search process.
Molecular mechanism concerning conformational changes of CDK2 mediated by binding of inhibitors using molecular dynamics simulations and principal component analysis

ABSTRACT Cyclin-dependent kinase 2 (CDK2) has been regarded as a promising drug target for anti-tumour agents. In this study, molecular dynamics (MD) simulations and principal component (PC) analysis were used to explore binding mechanism of three inhibitors 1PU, CDK, 50Z to CDK2 and influences of their bindings on conformational changes of CDK2. The results show that bindings of inhibitors yield obvious impacts on internal dynamics, movement patterns and conformational changes of CDK2. In addition, molecular mechanics generalized Born surface area (MM-GBSA) was applied to calculate binding free energies between three inhibitors and CDK2 and evaluate their binding ability to CDK2. The results show that CDK has the strongest binding to CDK2 among the current three inhibitors. Residue-based free energy decomposition method was further utilized to decode the contributions of a single residue to binding of inhibitors, and it was found that three inhibitors not only produce hydrogen bonding interactions and hydrophobic interactions with key residues of CDK2, which promotes binding of three inhibitors to CDK2, but also share similar binding modes. This work is expected to be helpful for design of efficient drugs targeting CDK2.
Application of the gamma‐interferon assay to determine the prevalence of bovine tuberculosis in slaughter livestock at abattoirs in Gauteng, South Africa

Abstract Background Bovine tuberculosis (bTB) is a zoonotic disease with great economic impact estimated at billions of dollars annually worldwide. Meat inspection represents a long‐standing form of disease surveillance that serves both food safety and animal health. The objective of this study was to determine the prevalence of bTB in livestock at abattoirs using a cell‐mediated immune (CMI) assay, the gamma interferon (IFN‐γ) assay. This cross‐sectional study was conducted at selected abattoirs (low‐throughput, high‐throughput and rural/informal) in Gauteng province, where animals were also subjected to routine meat inspection. Results A total of 410 fresh blood samples were collected from slaughter livestock (369 cattle and 41 sheep) from 15 abattoirs, and analysed using Bovigam® test kit with bovine, avian and Fortuitum purified protein derivatives (PPD) as blood stimulating antigens. The estimated prevalence of bTB in cattle was 4.4% (95% CI: 2.4%–7.3%). The prevalence of bTB in cattle varied between abattoirs (p = .005), ranging from 0% to 23%; however, there were no significant differences among genders, breeds, municipality, districts, origins of animals (feedlot, auction or farm) or throughput of abattoirs. The prevalence of avian reactors was 6.0% (95% CI: 3.6%–9.2%) in cattle, varying between abattoirs (p = .004) and ranging from 0% to 20.7%. None of the sheep with valid test results was positive for bTB and none was avian reactors (95% CI: 0%–15%). Conclusion The detection of bTB reactor cattle in our study clearly shows the limitation of disease surveillance using a meat inspection approach, as all the 410 slaughter animals sampled had passed visual abattoir inspection and been classified as bTB‐free. Our findings therefore emphasize the risk of zoonotic transmission of bTB to abattoir workers and potential food safety hazard to consumers. Furthermore, our study highlights the potential for the use of the IFN‐γ assay to reduce this risk.
Next‐generation sequencing for typing human papillomaviruses and predicting multi‐infections and their clinical symptoms

Human papillomavirus (HPV) has more than 100 different types, some of which are associated with cancer. The most common example is that of cervical cancer, which is associated with HPV16 and HPV18. Here, we performed next‐generation sequencing (NGS) to type 2436 samples obtained from Korean women to elucidate the correlation between multiple infections, virus types, and cytology. NGS revealed that types 58, 56, and 16 were the most common in high‐risk (HR) types, whereas types 90, 54, and 81 were the most common in low‐risk (LR) types. The incidence of atypical squamous cells of undetermined significance (ASCUS) or high‐grade squamous intraepithelial lesion (HSIL) was 11.45% in single‐type cases and 27.17% in multiple infections by the two types of HPV. ASCUS or HSIL was 29.79% in only the HR type multiple infections and 29.81% in mixed high‐ and low‐risk types of multiple infections, whereas it was 18.79% in LR type multiple infections (P ≤ 0.0001). Co‐infection by LR‐HPV and HR‐HPV is therefore more likely to cause cell lesions. Collectively, these results show that the higher the incidence of multiple infections, the greater the frequency of cell lesions. Thus, to predict the clinical symptoms, it would be beneficial to confirm the HPV type and multiple infections using NGS, although this could be relatively expensive.
A bionic soft tongue driven by shape memory alloy and pneumatics

Soft grippers have exhibited considerable advantages owing to their flexible deformation, compliant operation, and safe interaction with objects. The ability to grip solid and liquid objects can greatly expand the application range of the soft grippers. Dogs stick out their tongues and then curl them backward to form a ladle shape for eating food and drinking water. The large extension ratio and the ladling motion of the tongues endow dogs with flexible operations for both solids and liquids. In this work, inspired by both the extending and ladling motions of dog tongues, a bionic soft tongue with the capability of handling solid and liquid objects was designed. The bionic soft tongue was composed of a tongue base and a tongue tip, which were driven by pneumatics and shape memory alloy wires, respectively. The tongue base was capable of linearly elongating and contracting with a considerable scale, while the tongue tip could be curled into a ladle shape to grasp objects. The dynamic model of the tongue base was developed and then extended to build the model-based feedback controller for the motion control. A phase dynamic model of the tongue tip was simulated for structural optimization. With the model-based feedback controller, the bionic soft tongue could achieve a fast step response and precise position control. Experimental results showed that the bionic soft tongue could grasp different kinds of objects, including of water, rice, and gel balls. This work is expected to expand the application scope of soft grippers.