Miracle-dz/UniFAR-FTUs huggingface dataset:
Vaccines-1 Class Switching in Response to Gene Gun g T Cell Activation and IgH + Critical for CD8 Dermal Dendritic Cells Are + Langerin

The C-type lectin langerin/CD207 was originally discovered as a speciﬁc marker for epidermal Langerhans cells (LC). Recently, additional and distinct subsets of langerin + dendritic cells (DC) have been identiﬁed in lymph nodes and peripheral tissues of mice. Although the role of LC for immune activation or modulation is now being discussed controversially, other langerin + DC appear crucial for protective immunity in a growing set of infection and vaccination models. In knock-in mice that express the human diphtheria toxin receptor under control of the langerin promoter, injection of diphtheria toxin ablates LC for several weeks whereas other langerin + DC subsets are replenished within just a few days. Thus, by careful timing of diphtheria toxin injections selective states of deﬁciency in either LC only or all langerin + cells can be established. Taking advantage of this system, we found that, unlike selective LC deﬁciency, ablation of all langerin + DC abrogated the activation of IFN- g –producing and cytolytic CD8 + T cells after gene gun vaccination. Moreover, we identiﬁed migratory langerin + dermal DC as the subset that directly activated CD8 + T cells in lymph nodes. Langerin + DC were also critical for IgG1 but not IgG2a Ab induction, suggesting differential polarization of CD4 + T helper cells by langerin + or langerin-negative DC, respectively. In contrast, protein vaccines administered with various adjuvants induced IgG1 independently of langerin + DC. Taken together, these ﬁndings reﬂect a highly specialized division of labor between different DC subsets both with respect to Ag encounter as well as downstream processes of immune activation. The Journal of Immunology , 2011,
Construction of a short version of the Montreal Cognitive Assessment (MoCA) rating scale for the Thai population using Partial Least Squares analysis

Abstract Background The Montreal Cognitive Assessment (MoCA) rating scale is frequently used to assess cognitive impairments in amnestic mild cognitive impairment (aMCI) and Alzheimer’s disease (AD). Objectives The aims of this study were to a) evaluate the construct validity of the MoCA and its subdomains or whether the MoCA can be improved by feature reduction, and b) develop a short version of the MoCA (MoCA-Brief) for the Thai population. Methods We recruited 181 participants, namely 60 healthy controls, 61 aMCI, and 60 AD patients. Results The construct reliability of the original MoCA was not optimal and could be improved by deleting one subdomain (Naming) and five items, namely Clock Circle, Lion, Digit Forward, Repeat 2nd Sentence, and Place, which showed inadequate loadings on their latent vectors. To construct the MoCA-Brief, the reduced model underwent further reduction and feature selection based on model quality data of the outer models. We produced a MoCA-Brief rating scale comprising five items, namely Clock Time, Subtract 7, Fluency, Month, and Year. The first latent vector extracted from these five indicators showed adequate construct validity with an Average Variance Extracted of 0.599, composite reliability of 0.822, Cronbach’s alpha of 0.832 and rho A of 0.833. The MoCA-Brief factor score showed a strong correlation with the total MoCA score (r = 0.98, p < 0.001) and shows adequate concurrent, test-retest, and inter-rater validity. Conclusion The construct validity of the MoCA may be improved by deleting five items. The new MoCA-Brief rating scale deserves validation in independent samples and especially in other countries.
SeraColostrum and Maternal and Newborn Cord Autoantibody Repertoires in Healthy Tumor-Associated and Disease-Associated

In this work, we studied autoantibody repertoires and Ig isotypes in 71 mothers and their 104 healthy newborns (including twins and triplets delivered term or premature). Newborns receive maternal IgG Abs via the placenta before birth, but developing infants must produce their own IgM and IgA Abs. We used an Ag microarray analysis to detect binding to a selection of 295 self-Ags, compared with 27 standard foreign Ags. The magnitude of binding to speciﬁc self-Ags was found to be not less than that to the foreign Ags. As expected, each newborn shared with its mother a similar IgG repertoire—manifest as early as the 24th week of gestation. IgM and IgA autoantibody repertoires in cord sera were highly correlated among the newborns and differed from their mothers’ repertoires; the latter differed in sera and milk. The autoantibodies bound to self-Ags known to be associated with tumors and to autoimmune diseases. Thus, autoantibody repertoires in healthy humans—the immunological homunculus—arise congenitally, differ in maternal milk and sera, and mark the potential of the immune system to attack tumors, beneﬁcially, or healthy tissues, harmfully; regulation of the tissue site, the dynamics, and the response phenotype of homuncular autoimmunity very likely affects health. The Journal of Immunology , 2015, 194: 000–000.
Technology and design of integrated circuits for up to 50 V applications

This paper presents the challenges of developing an integrated circuit process suitable for high performance analog and digital circuits together with circuits operating at up to 50 V. An economic submicron CMOS technology is described which meets these challenges. Attractive application areas for this process include intelligent motor control, capacitative transducers (such as MEMS based devices), and devices operating in industrial environments where the supply voltage busses are in the 12 V to 50 V range. Specific circuit designs for these applications are discussed.
A SURVEY ON GENERATION OF MOBILE WIRELESS TECHNOLOGY

Now days, Mobile has become very essential, can say necessary or important part of our daily life. To transfer the information between two or more points which are not connected by any electrical conductor that is wireless communication. The distance may be short or long. It consists of various types of fixed mobiles, cellular telephones, portable radios, PDAs. In this paper, we will survey on the development of different mobile wireless technologies along with their advantages of one over the other. In the past mobile wireless technology have experience 4 or 5 generation that is 0G to 4G.Nowdays mobile wireless technology Focus on new challenging research on advanced implementation of 4G technology and 5G technology. Now 5G is not officially used. Concept is only theory not real.
Safety enhancement in robotic neurosurgery through vessel tracking

S. Moccia, F. Prudente, E. De Momi , C. Riviere, A. Perin, R. Sekula, L. S. Mattos Department of Advanced Robotics, Istituto Italiano di Tecnologia, Genoa, Italy Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milan, Italy Robotics Institute, Carnegie Mellon University, Pittsburgh, USA Besta NeuroSim Center, IRCCS Istituto Neurologico C. Besta, Milan, Italy Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, USA sara.moccia@iit.it
Detecting Well-being in Digital Communities: An Interdisciplinary Engineering Approach for its Indicators

In this thesis, the challenges of defining, refining, and applying well-being as a progressive management indicator are addressed. This work's implications and contributions are highly relevant for service research as it advances the integration of consumer well-being and the service value chain. It also provides a substantial contribution to policy and strategic management by integrating constituents' values and experiences with recommendations for progressive community management.
Multi-Dimensional Tele-healthcare Engineering Undergraduate Education via Building-Block-based Medical Sensor Labs

1 – The entire world is facing healthcare challenges. Human society is in critical need of trained telehealthcare engineers due to the fast expanding bioengineering industries. In a project (sponsored by U.S. National Science Foundation), we are developing a new course called ECE 493 Tele-healthcare Computing. This paper reports our lab design and teaching experiences. Especially we will discuss our educational development of medical networks and bio-signal processing. We have designed three class labs on ECG sensor and ECG signal processing. Those class labs are developed from a building-block approach. When we offer the lectures to students, we have used a multi-dimensional approach: Dimension-1: Multi-student-level adaptive materials: To meet different schools’ course setup requirements, we design basic, intermediate and advanced labs for different levels of undergraduate students. Dimension-2: Medical-application-driven, practical learning: Engineering students show much greater enthusiasm to materials that are closely connected to their lives (i.e. application-driven learning) than pure theoretical lab topics (such as writing a program to verify an algorithm). Dimension-3: Multi-solution-based, creative engineering learning: We propose to use level-to-level questionbased, non-instructional lab style to motivate students to seek for solutions from out-of-classroom materials such as web resources. And we will make flexible grading policy to encourage students’ out-of-box thinking and creative engineering designs. Index Words – Tele-healthcare engineering, undergraduate education, Building Blocks, Labs, embedded system
In Vivo Bioactivities of Hoya parasitica (Wall.) and In Silico Study against Cyclooxygenase Enzymes

Hoya parasitica (Wall.) is extensively used in traditional medicine for the treatment of various diseases including rheumatism, kidney problems, jaundice, urinary tract disorders, fever, and pain. The present study was designed to explore new lead compound(s) to alleviate pain, pyresis, and diarrhea from methanol, ethyl acetate, and n-hexane extracts of H. parasitica (Wall.) leaves (MHP, EAHP, and NHP, respectively). Analgesic activity of the extracts was assessed through acetic acid induced writhing, tail immersion, and hot plate tests while brewer's yeast-induced pyrexia test was employed for the assessment of antipyretic activity. Besides, castor oil and magnesium sulfate induced diarrheal tests were utilized for the evaluation of antidiarrheal properties. Moreover, in silico study of the isolated compounds was undertaken to seek out best-fit phytoconstituent(s) against cyclooxygenase enzymes. MHP revealed substantial antioxidant activities in different in vitro assays compared to EAHP and NHP. In the acetic acid-induced writhing test, among the extracts, MHP (400 mg/kg) revealed maximum 74.15 ± 1% inhibition of writhing comparable to that of standard (85.77 ± 1.39%). Again, in tail immersion and hot plate tests, higher doses of all the test samples exhibited a significant increase of latent period in a time-dependent manner. In brewer yeast-induced pyrexia test, at 3rd and 4th hour of treatment, significant (P < 0.05) antipyretic action was found in the test samples. In both castor oil and magnesium induced diarrheal tests, MHP at 400 mg/kg showed the highest percent inhibition of diarrhea (68.62 ± 4.74 and 64.99 ± 2.90, respectively). Moreover, molecular docking analysis corroborated the results of the present study. The findings of the present study supported the traditional uses of this plant for the alleviation of pain and fever. Furthermore, hoyasterone was found to be the most effective lead compound as cyclooxygenase enzyme inhibitor.
Association of Postoperative Lateral Meniscal Extrusion With Cartilage Degeneration on Magnetic Resonance Imaging After Discoid Lateral Meniscus Reshaping Surgery

Background: Although arthroscopic reshaping surgery for a discoid lateral meniscus (DLM) has good clinical results, it cannot completely prevent degeneration. The degree of DLM extrusion associated with degenerative changes is unclear. Purpose/Hypothesis: To measure meniscal extrusion preoperatively and postoperatively in patients who underwent DLM-reshaping surgery and examine factors associated with knee articular cartilage degeneration. It was hypothesized that meniscal extrusion existed preoperatively, progressed postoperatively, and was related to knee joint degeneration. Study Design: Case-control study; Level of evidence, 3. Methods: We retrospectively reviewed the medical records of patients who underwent DLM-reshaping surgery and attended ≥2 years of follow-up. Magnetic resonance imaging (MRI) was performed preoperatively and at 24 months postoperatively, and residual midbody meniscal extrusion was measured. Cartilage degeneration was detected when the Whole-Organ Magnetic Resonance Imaging Score (WORMS) of the lateral compartment was grade ≥3 at 2 years postoperatively. Factors associated with MRI cartilage degeneration were evaluated. Results: Included in this study were 48 knees in 39 patients; the mean patient age at the time of surgery was 12.0 years. The mean midbody meniscal extrusion significantly increased from 0.8 mm preoperatively to 1.6 mm at 24 months postoperatively (P < .001). According to the WORMS cartilage score, 16 patients were categorized as having MRI cartilage degeneration. Multivariate logistic analysis showed that an inferior preoperative Lysholm score (odds ratio, 0.89; P = .024) and postoperative extrusion (odds ratio, 6.18; P = .010) significantly increased the risk of cartilage degeneration. The receiver operating characteristic curve showed that a residual meniscal extrusion of 2.0 mm was the cutoff value indicating cartilage degeneration (sensitivity, 87.5%; specificity, 78.1%). Conclusion: DLM extrusion significantly increased from 0.8 mm preoperatively to 1.6 mm at 2 years postoperatively. Postoperative extrusion and a lower preoperative Lysholm score were factors related to MRI cartilage degeneration postoperatively. A postoperative extrusion of 2.0 mm was the cutoff value for MRI cartilage degeneration.
Identification of Bioactive Components of Stephania epigaea Lo and Their Potential Therapeutic Targets by UPLC-MS/MS and Network Pharmacology

Stephania epigaea, an important traditional folk medicinal plant, elucidating its bioactive compound profiles and their molecular mechanisms of action on human health, would better understand its traditional therapies and guide their use in preclinical and clinical. This study aims to detect the critical therapeutic compounds, predict their targets, and explore potential therapeutic molecular mechanisms. This work first determined metabolites from roots, stems, and flowering twigs of S. epigaea by a widely targeted metabolomic analysis assay. Then, the drug likeness of the compounds and their pharmacokinetic profiles were screened by the ADMETlab server. The target proteins of active compounds were further analyzed by PPI combing with GO and KEGG cluster enrichment analysis. Finally, the interaction networks between essential compounds, targets, and disease-associated pathways were constructed, and the essential compounds binding to their possible target proteins were verified by molecular docking. Five key target proteins (EGFR, HSP90AA1, SRC, TNF, and CASP3) and twelve correlated metabolites, including aknadinine, cephakicine, homostephanoline, and N-methylliriodendronine associated with medical applications of S. epigaea, were identified, and the compounds and protein interactions were verified. The key active ingredients are mainly accumulated in the root, which indicates that the root is the main medicinal tissue. This study demonstrated that S. epigaea might exert the desired disease efficacy mainly through twelve components interacting via five essential target proteins. EGFR is the most critical one, which deserves further verification by biological studies.
Genome-Wide Identification and Functional Analysis of Lysine Histidine Transporter (LHT) Gene Families in Maize

Amino acid transporters (AATs) are essential membrane proteins that transfer amino acids across cells. They are necessary for plant growth and development. The lysine histidine transporter (LHT) gene family in maize (Zea mays) has not yet been characterized. According to sequence composition and phylogenetic placement, this study found 15 LHT genes in the maize genome. The ZmLHT genes are scattered across the plasma membrane. The study also analyzed the evolutionary relationships, gene structures, conserved motifs, 3D protein structure, a transmembrane domain, and gene expression of the 15 LHT genes in maize. Comprehensive analyses of ZmLHT gene expression profiles revealed distinct expression patterns in maize LHT genes in various tissues. This study's extensive data will serve as a foundation for future ZmLHT gene family research. This study might make easier to understand how LHT genes work in maize and other crops.
The complete chloroplast genome of Camellia leyeensis (theaceae)

Abstract Camellia leyeensis Chang ＆ Y. C. Zhong is a plant belonging to the genus Camellia. To determine its correct taxonomic status and better understand its molecular phylogenetic and genetic diversity, we studied the chloroplast genome of this species. Here, we report and characterize the complete chloroplast (cp) genome of C. leyeensis by using Illumina paired-end sequencing data. The chloroplast genome was determined to be 157,063 bp in length with a GC content of 37.30%. The genome contained 136 genes, including 91 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The sequence contained a large single-copy region (LSC, 86,661 bp), a small single-copy region (SSC, 18,284 bp), and two inverted repeat sequences (IRs, 26,059 bp, each). The GC content of the IR regions (42.96%) was higher than that of the SSC region (30.6%) and LSC region (35.31%). Phylogenetic analysis suggested that Camellia leyeensis is closely related to C. anlungensis with 96% bootstrap support. This chloroplast genome would be helpful for the phylogeny and conservation of Camellia.
Feature tuning improves MAXENT predictions of the potential distribution of Pedicularis longiflora Rudolph and its variant

Pedicularis longiflora Rudolph and its variant (P. longiflora var. tubiformis (Klotzsch) Tsoong) are alpine plants and traditional Chinese medicines with important medicinal value, and future climate changes may have an adverse impact on their geographic distribution. The maximum entropy (MAXENT) model has the outstanding ability to predict the potential distribution region of species under climate change. Therefore, given the importance of the parameter settings of feature classes (FCs) and the regularization multiplier (RM) of the MAXENT model and the importance of add indicators to evaluate model performance, we used ENMeval to improve the MAXENT niche model and conducted an in-depth study on the potential distributions of these two alpine medicinal plants. We adjusted the parameters of FC and RM in the MAXENT model, evaluated the adjusted MAXENT model using six indicators, determined the most important ecogeographical factors (EGFs) that affect the potential distributions of these plants, and compared their current potential distributions between the adjusted model and the default model. The adjusted model performed better; thus, we used the improved MAXENT model to predict their future potential distributions. The model predicted that P. longiflora Rudolph and its variant (P. longiflora var. tubiformis (Klotzsch) Tsoong) would move northward and showed a decrease in extent under future climate scenarios. This result is important to predict their potential distribution regions under changing climate scenarios to develop effective long-term resource conservation and management plans for these species.
La thymoglobuline en traitement d’induction chez les transplantés rénaux à faible risque immunologique: une expérience marocaine

Introduction la thymoglobuline® est un anticorps polyclonal indiqué en traitement d´induction en transplantation rénale. Le but de notre travail est d´étudier l´efficacité de la thymoglobuline® en traitement d´induction chez des transplantés rénaux à faible risque immunologique. Méthodes il s´agit d´une étude rétrospective entre Janvier 2012 et Septembre 2017. On a inclus les patients à faible risque immunologique, défini par l´absence de transplantation antérieure et d´anticorps spécifiques du donneur (DSA), qui ont reçu un traitement d´induction à base de thymoglobuline®. On a étudié les caractéristiques démographiques et cliniques, les paramètres biologiques ainsi que les complications post transplantation rénale. Résultats nous avons inclus 55 patients transplantés rénaux avec un suivi moyen de 38 ± 16 mois. L´âge moyen était de 39,1 ± 12,1 ans avec une prédominance masculine (58,2%). Aucun patient n´avait de DSA avant la greffe. La dose cumulative de la thymoglobuline® était de 4,26 ± 0,87 mg/kg avec une durée moyenne de 5 ± 0,82 jours. La déplétion lymphocytaire était maximale au premier jour de la perfusion. Nous avons recensé 3 cas de reprise retardée de la fonction du greffon, au moins un épisode d´infection bactérienne chez 56,4% de nos patients, 7 cas d´infection à cytomégalovirus (CMV) (12,7%) et 2 cas de maladie à CMV (3,6%). La survie du greffon était notée chez tous nos patients avec un taux de créatinine sérique moyen à 11,7 ± 3,6 mg/l au cours de la dernière consultation. Conclusion bien qu´elle ne soit pas indiquée en première intention chez les patients à faible risque immunologique, la thymoglobuline® peut néanmoins être prescrite à dose plus faible, avec une efficacité similaire et sans exposition à un risque de rejet plus élevé.
Regulation of Mitochondrial Homeostasis and Nrf2 in Kidney Disease: Timing Is Critical

Abnormal regulation of mitochondrial homeostasis plays a critical role in the progression of renal disease. Recent studies have shown that activation of nuclear factor erythroid 2-related factor 2 (Nrf2) has time-dependent protective effects, which can be explained by the differing regulation of mitochondrial homeostasis during the various stages of kidney disease. In this review, we summarize the mechanisms whereby mitochondrial homeostasis is regulated and the nature of the dysregulation of mitochondrial homeostasis in renal disease. In addition, we summarize the dual roles of Nrf2 in kidney disease by discussing the studies that have shown the importance of the timing of its activation in the regulation of mitochondrial homeostasis. This should provide a theoretical basis for therapeutic strategies aimed at activating Nrf2 in kidney disease.
Synthesis, Characterization, Functionalization and Bio-Applications of Hydroxyapatite Nanomaterials: An Overview

Abstract Hydroxyapatite (HA) is similar to natural bone regarding composition, and its structure favors in biomedical applications. Continuous research and progress on HA nanomaterials (HA-NMs) have explored novel fabrication approaches coupled with functionalization and characterization methods. These nanomaterials have a significant role in many biomedical areas like sustained drug and gene delivery, bio-imaging, magnetic resonance, cell separation, and hyperthermia treatment due to their promising biocompatibility. This review highlighted the HA-NMs chemical composition, recent progress in synthesis methods, characterization and surface modification methods, ion-doping, and role in biomedical applications. HA-NMs have a substantial role as drug delivery vehicles, coating material, bone implant, coating, ceramic, and composite materials. Here, we try to summarize an overview of HA-NMs with the provision of future directions.