Miracle-dz/UniFAR-FTUs huggingface dataset:
Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Enzymatic detachment of cells might damage important features and functions of cells and could affect subsequent cell‐based applications. Therefore, nonenzymatic cell detachment using thermosensitive polymer matrix is necessary for maintaining cell quality after harvesting. In this study, we prepared thermosensitive PNIPAm‐co‐AAc‐b‐PS and PNIPAm‐co‐AAm‐b‐PS copolymers and low critical solution temperature (LCST) was tuned near to body temperature. Then, spin coated polymer films were prepared for cell adhesion and thermal‐induced cell detachment. The alpha‐step analysis and scanning electron microscope image of the films suggested that the thickness of the films depends on the molecular weight and concentration which ranged from 206 to 1330 nm for PNIPAm‐co‐AAc‐b‐PS and 97.5–497 nm for PNIPAm‐co‐AAm‐b‐PS. The contact angles of the films verified that the polymer surface was moderately hydrophilic at 37°C. Importantly, RAW264.7 cells were convincingly proliferated on the films to a confluent of >80% within 48 h and abled to detach by reducing the temperature. However, relatively more cells were grown on PNIPAm‐co‐AAm‐b‐PS (5%w/v) films and thermal‐induced cell detachment was more abundant in this formulation. As a result, PNIPAm‐co‐AAm‐b‐PS (5%w/v) was further used to coat commercial cytodex 3 microcarriers for 3D cell culturing and interestingly enhanced cell detachment with preserved potential of recovery was observed at a temperature of below LCST. Thus, surface modification of microcarriers with thermosensitive PNIPAm‐co‐AAm‐b‐PS could be vital strategy for nonenzymatic cell detachment and to achieve adequate number of cells with maximum cell viability and functionality.
Exploring Behavioural Activation as a treatment for low mood within CAMHS: An IPA study of adolescent experiences

Low mood is the most commonly diagnosed mental health condition affecting adolescents; however, it remains complex to treat due to multi-systemic risk and maintaining factors. Behavioural Activation (BA) is a brief therapy which demonstrates promising treatment outcomes, although limited qualitative accounts exist of how adolescents experience this. This is one of the first studies undertaken in Child and Adolescent Mental Health Services (CAMHS) to explore the perspectives of adolescent’s with low mood who have received BA therapy. Interpretative phenomenological analysis was conducted from one-to-one interviews with nine adolescents who received BA, generating an idiographic account of their experiences. Three superordinate themes emerged: how the format of BA can promote the integration of coping skills into one’s life; how interpersonal connections and therapeutic relationships may improve intervention outcomes; and how BA principles could be internalised as part of a young person’s day-to-day life. Participants valued the structure and flexibility of the manualised approach, forming an alliance with the therapist, and enhancing interpersonal relationships. This study details how BA can enhance resiliency skills for adolescents experiencing low mood and illustrates some of the change process at inter and intrapersonal levels, which should guide further youth-led research.
Feeding the European hedgehog (Erinaceus europaeus L.)-risks of commercial diets for wildlife.

The supplemental feeding of wildlife is a worldwide phenomenon. The most widespread and socially accepted example is bird feeding. Commercial hedgehog (Erinaceus europaeus L.) diets are readily available in Switzerland. To test the hypothesis that commercial hedgehog diets are nutritionally inadequate, the following parameters (as taken from the label) of five commercial diets (three dry and two wet) were compared to the natural diet of E. europaeus: List of feed materials, crude ash (CA), crude protein (CP), crude fibre (CF), ether extract (EE) and nitrogen-free extracts (NfE). To test the second hypothesis, that feed labels of commercially available hedgehog diets in Switzerland are in accordance with Swiss and European law, the following parameters were assessed: Type of feed, net quantity, moisture content, instructions for proper use, list of the feed materials and claims. In all dry diets, the first ingredient was cereals (38.8%-50%), whereas in both wet diets, it was meat and meat by-products (51% in one product). Only one product had a CP content comparable to that of the natural diet and four products had a higher NfE content. Overall, the wet products were more comparable to the natural diet, especially regarding CP and NfE content. Of the five examined diets, three were labelled as complete feeds and two as compound feeds. The label analysis revealed inaccuracies and possible misleading information on two products. In conclusion, the use of commercial dry hedgehog diets should be discouraged as they do not resemble the natural diet of E. europaeus and are likely nutritionally inadequate. The commercial wet hedgehog diets should only be used supplementary. The feed labels of commercially available hedgehog diets were not always in compliance with Swiss and European law. The public should be educated about feeding E. europaeus and the risks of commercial diets for wildlife.
Association between socioeconomic status and chronic obstructive pulmonary disease in Jiangsu province, China: a population-based study

Supplemental Digital Content is available in the text Abstract Background: Chronic obstructive pulmonary disease (COPD) is a common public health problem worldwide. Recent studies have reported that socioeconomic status (SES) is related to the incidence of COPD. This study aimed to investigate the association between SES and COPD among adults in Jiangsu province, China, and to determine the possible direct and indirect effects of SES on the morbidity of COPD. Methods: A cross-sectional study was conducted among adults aged 40 years and above between May and December of 2015 in Jiangsu province, China. Participants were selected using a multistage sampling approach. COPD, the outcome variable, was diagnosed by physicians based on spirometry, respiratory symptoms, and risk factors. Education, occupation, and monthly family average income (FAI) were used to separately indicate SES as the explanatory variable. Mixed-effects logistic regression models were introduced to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for examining the SES-COPD relationship. A pathway analysis was conducted to further explore the pulmonary function impairment of patients with different SES. Results: The mean age of the 2421 participants was 56.63 ± 9.62 years. The prevalence of COPD was 11.8% (95% CI: 10.5%–13.1%) among the overall sample population. After adjustment for age, gender, residence, outdoor and indoor air pollution, body weight status, cigarette smoking, and potential study area-level clustering effects, educational attainment was negatively associated with COPD prevalence in men; white collars were at lower risk (OR: 0.60, 95% CI: 0.43–0.83) of experiencing COPD than blue collars; compared with those within the lower FAI subgroup, participants in the upper (OR: 0.68, 95% CI: 0.49–0.97) tertiles were less likely to experience COPD. Such negative associations between all these three SES indicators and COPD were significant among men only. Education, FAI, and occupation had direct or indirect effects on pulmonary function including post-bronchodilator forced expiratory volume in 1 s/forced vital capacity (FEV1/FVC), FEV1, FVC, and FEV1 percentage of predicted. Education, FAI, and occupation had indirect effects on pulmonary function indices of all participants mainly through smoking status, indoor air pollution, and outdoor air pollution. We also found that occupation could affect post-bronchodilator FEV1/FVC through body mass index. Conclusions: Education, occupation, and FAI had an adverse relationship with COPD prevalence in Jiangsu province, China. SES has both direct and indirect associations with pulmonary function impairment. SES is of great significance for COPD morbidity. It is important that population-based COPD prevention strategies should be tailored for people with different SES.
What factors are associated with food security among recently arrived refugees resettling in high-income countries? A scoping review

Abstract Objective: Refugees are vulnerable to food insecurity (FI). This is attributable to a combination of inequitable social determinants and cultural differences. In 2019, 92 % of refugee resettlement (host country provides residency/citizenship) occurred in high-income countries, but little is known about the factors impacting their food security status in this setting. The review’s objective was to therefore thematically identify factors affecting food security among refugees resettling in high-income countries. Design: This review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews. Between May–July 2020 and February 2021, peer-reviewed studies focused on FI, and published in English from 2000–2020, were searched on Medline, CINAHL, Scopus, Informit, PsychArticles, Proquest and EmBase. Setting: Only studies set in high-income countries were included. Participants: Fifty percent or more of study participants had to be refugees who had resettled within 5 years. Results: Twenty studies from six high-income countries were included. Culturally based food practices and priorities, confidence in navigating local foodways and transport, level of community connections and capabilities in local language and food preparation were key themes associated with food security. Conclusions: Utilising the four themes of culture, confidence, community and capabilities, there is an opportunity to improve the cultural sensitivity of measurement tools, develop understanding of how community-based resources (such as social capital) can be leveraged as food security buffers and modify existing food security initiatives to better serve refugee needs.
Disentangling The Link Between Leaf Photosynthesis And Turgor On Fruit Growth.

Despite the importance of understanding plant growth, the mechanisms underlying how plant and fruit growth declines, during drought remains poorly understood. Specifically, it remains unresolved whether carbon or water factors are responsible for limiting growth as drought progresses. We examine questions regarding the relative importance of water and carbon to fruit growth depending on the water deficit level and the fruit growth stage, by measuring fruit diameter, leaf photosynthesis and a proxy of cell turgor in olive. Flow cytometry was also measured to determine the fruit cell division stage. We found that photosynthesis and turgor were related to fruit growth; specifically, the relative importance of photosynthesis was higher during periods of more intense cell division, while turgor had higher relative importance in periods where cell division comes close to ceasing, and fruit growth is dependent mainly on cell expansion. This pattern was found regardless of the water deficit level, although, turgor and growth ceased at more similar values of leaf water potential than photosynthesis. Cell division occurred even when fruit growth seems to stop under water deficit conditions, which likely helped fruits to grow disproportionately when trees were hydrated again, compensating for periods with low-turgor. As a result, the final fruit size was not severely penalized. We conclude that carbon and water processes are able to explain fruit growth, with importance placed on the combination of cell division and expansion. However, the major limitation to growth is turgor, which adds evidence to the sink limitation hypothesis.
Tailoring the Growth Rate and Surface Facet for Synthesis of High-Quality Continuous Graphene Films from CH4 at 750 °C via Chemical Vapor Deposition

Previous work has demonstrated that the growth temperature for the chemical vapor deposition of graphene can be decreased by tailoring the precursor composition. Here, we fix the precursor as CH4 and instead explore the effects of the catalyst facet, synthesis conditions, and growth rate on the quality of graphene grown at reduced temperature on Cu. We find that in order to obtain graphene films with low defect density, it is critical to maintain a slow growth rate, which is achieved using a low CH4 partial pressure. Furthermore, growth on Cu(111) is more efficient than on other low index Cu facets. Using optimized growth conditions, we achieve high-quality continuous monolayer graphene films, with a low nucleation density of 4 × 10–2 μm–2 and a negligible Raman D:G ratio, at 750 °C, which is 125 °C cooler than previous reports achieving full graphene coverage on Cu. The field-effect mobility is 2600 cm2 V–1 s–1 at a carrier concentration of 1012 cm–2, and low-energy electron microscopy indicates that the.
Isolation of epizootic hemorrhagic disease virus serotype 7 from cattle showing fever in Japan in 2016 and improvement of a reverse transcription-polymerase chain reaction assay to detect epizootic hemorrhagic disease virus

Epizootic hemorrhagic disease (EHD) is an arthropod-borne disease of wild and domestic ruminants caused by the EHD virus (EHDV). To date, seven EHDV serotypes have been identified. In Japan, strain Ibaraki of EHDV serotype 2 has caused outbreaks of Ibaraki disease in cattle. In addition, EHDV serotype 7 (EHDV-7) has caused large-scale EHD epizootics. In mid-September 2016, eight cattle at a breeding farm in Fukuoka Prefecture, Japan developed fever. Since EHDV-7 was detected in sentinel cattle in western Japan in 2016, we suspected that the cause of this fever might be an EHDV-7 infection. In this study, we tested cattle for EHDV-7 and some other viruses. Consequently, EHDV was isolated from washed blood cells collected from three of the eight cattle, and genetic analysis of genome segment 2 revealed that this isolate was EHDV-7. Moreover, all affected cattle tested positive for anti-EHDV-7 neutralizing antibodies. Our results suggest that the fever was caused by EHDV-7 infection. In addition, we modified a conventional reverse transcription polymerase chain reaction assay for the specific detection of EHDV. This modified assay could detect various strains of EHDV isolated in Japan, Australia, and North America. Furthermore, the assay permitted the detection of EHDV-7 in blood cells collected from seven of the eight cattle. We believe that this modified assay will be a useful tool for the diagnosis of EHD.
Relative water content consistently predicts drought mortality risk in seedling populations with different morphology, physiology, and times to death

Predicted increases in forest drought mortality highlight the need for predictors of incipient drought-induced mortality (DIM) risk that enable proactive large-scale management. Such predictors should be consistent across plants with varying morphology and physiology. Because of their integrative nature, indicators of water status are promising candidates for real time monitoring of DIM, particularly if they standardize morphological differences among plants. We assessed the extent to which differences in morphology and physiology between Pinus ponderosa populations influence time to mortality and the predictive power of key indicators of DIM risk. Time to incipient mortality differed between populations but occurred at the same relative water content (RWC) and water potential (WP). RWC and WP were accurate predictors of drought mortality risk. These results highlight that variables related to water status capture critical thresholds during DIM and the associated dehydration processes. Both WP and RWC are promising candidates for large-scale assessments of DIM risk. RWC is of special interest because it allows comparisons across different morphologies and can be remotely sensed. Our results offer promise for real-time landscape-level monitoring of DIM and its global impacts in the near term.
A rice LRR receptor‐like protein associates with its adaptor kinase OsSOBIR1 to mediate plant immunity against viral infection

Summary Plants sense pathogen attacks using a variety of receptors at the cell surface. The LRR receptor‐like proteins (RLP) and receptor‐like kinases (RLK) are widely reported to participate in plant defence against bacterial and fungal pathogen invasion. However, the role of RLP and RLK in plant antiviral defence has rarely been reported. We employed a high‐throughput‐sequencing approach, transgenic rice plants and viral inoculation assays to investigate the role of OsRLP1 and OsSOBIR1 proteins in rice immunity against virus infection. The transcript of a rice LRR receptor‐like protein, OsRLP1, was markedly up‐regulated following infection by RBSDV, a devastating pathogen of rice and maize. Viral inoculation on various OsRLP1 mutants demonstrated that OsRLP1 modulates rice resistance against RBSDV infection. It was also shown that OsRLP1 is involved in the RBSDV‐induced defence response by positively regulating the activation of MAPKs and PTI‐related gene expression. OsRLP1 interacted with a receptor‐like kinase OsSOBIR1, which was shown to regulate the PTI response and rice antiviral defence. Our results offer a novel insight into how a virus‐induced receptor‐like protein and its adaptor kinase activate the PTI response and antiviral defence in rice.
Canine junctional epidermolysis bullosa due to a novel mutation in LAMA3 with severe upper respiratory involvement.

BACKGROUND Junctional epidermolysis bullosa (JEB) is a group of congenital blistering skin diseases characterized by clefting through the lamina lucida of the basement membrane zone. OBJECTIVES To characterize the clinical and morphological features of a congenital mechanobullous disease in a litter of puppies with severe upper respiratory involvement, and to identify an associated genetic variant. ANIMALS Five of eight puppies in an Australian cattle dog cross-bred litter showed signs of skin fragility. Three were stillborn and one died at one month of age. The two surviving puppies were presented with blistering skin disease and severe respiratory distress. Additionally, one unaffected sibling was examined and blood was obtained for genetic testing. METHODS AND MATERIALS Post-mortem examination, histopathological evaluation and electron microscopy were performed. Whole genome sequencing (WGS) of one affected puppy was compared to a database of 522 dogs of 55 different breeds for variant analysis. Sanger sequencing of one additional affected and one unaffected sibling confirmed the variant. RESULTS Clinically, severe mucocutaneous ulcers occurred in frictional areas with claw sloughing. Histopathological results revealed subepidermal clefts and electron microscopy confirmed the split in the lamina lucida. Post-mortem examination documented extensive pharyngeal and laryngeal lesions with granulation tissue and fibrinous exudate obscuring the airway. Moderate tracheal hypoplasia contributed. The WGS revealed a novel missense variant in the laminin α3-chain XP_537297. 2p(Asp2867Val), with an autosomal recessive mode of inheritance. CONCLUSIONS AND CLINICAL RELEVANCE A novel variant in LAMA3 caused a generalized and severe phenotype of JEB with an unique clinical presentation of upper airway obstruction.
Mechanism and Kinetics of CO2 Adsorption on Surface Bonded Amines

The impact of H2O on the mechanism and kinetics of CO2 adsorption by amine-impregnated SBA-15 has been investigated. The H2O-free adsorption mechanism proceeds predominantly via formation of carbamates stabilized between two amine groups. Bicarbonates and surface stabilized carbamates were formed on hydrated sorbents. Film diffusion limitations were not observed during adsorption of CO2 at high amine loadings. Gaseous water decreased the adsorption rate but increased the maximum equilibrium uptake as well as the uptake before breakthrough of the reactor bed. A water film is formed on the adsorbent particles in gas streams containing more than 5 vol %, gaseous H2O limiting the interaction of CO2 with the active amine sites and constraining the rates of adsorption. The higher uptake of CO2 in the presence of H2O vapor is a result of a change in the adsorption mechanism that increases the amine efficiency, thus leading to a higher adsorption capacity. The adsorption was fully reversible for all adsorbents at.
Dopamine modulations of reward‐driven music memory consolidation

Music listening provides one of the most significant abstract rewards for humans because hearing music activates the dopaminergic mesolimbic system. Given the strong link between reward, dopamine, and memory, we aimed here to investigate the hypothesis that dopamine‐dependent musical reward can drive memory improvements. Twenty‐nine healthy participants of both sexes provided reward ratings of unfamiliar musical excerpts that had to be remembered following a consolidation period under three separate conditions: after the ingestion of a dopaminergic antagonist, a dopaminergic precursor, or a placebo. Linear mixed modeling of the intervention data showed that the effect of reward on memory—i.e., the greater the reward experienced while listening to the musical excerpts, the better the memory recollection performance—was modulated by both dopaminergic signaling and individual differences in reward processing. Greater pleasure was consistently associated with better memory outcomes in participants with high sensitivity to musical reward, but this effect was lost when dopaminergic signaling was disrupted in participants with average or low musical hedonia. Our work highlights the flexibility of the human dopaminergic system, which can enhance memory formation not only through explicit and/or primary reinforcers but also via abstract and aesthetic rewards such as music.
Asthma‐inducing potential of 28 substances in spray cleaning products—Assessed by quantitative structure activity relationship (QSAR) testing and literature review

Exposure to spray cleaning products constitutes a potential risk for asthma induction. We set out to review whether substances in such products are potential inducers of asthma. We identified 101 spray cleaning products for professional use. Twenty‐eight of their chemical substances were selected. We based the selection on (a) positive prediction for respiratory sensitisation in humans based on quantitative structure activity relationship (QSAR) in the Danish (Q)SAR Database, (b) positive QSAR prediction for severe skin irritation in rabbits and (c) knowledge on the substances' physico‐chemical characteristics and toxicity. Combining the findings in the literature and QSAR predictions, we could group substances into four classes: (1) some indication in humans for asthma induction: chloramine, benzalkonium chloride; (2) some indication in animals for asthma induction: ethylenediaminetetraacetic acid (EDTA), citric acid; (3) equivocal data: hypochlorite; (4) few or lacking data: nitriloacetic acid, monoethanolamine, 2‐(2‐aminoethoxy)ethanol, 2‐diethylaminoethanol, alkyldimethylamin oxide, 1‐aminopropan‐2‐ol, methylisothiazolinone, benzisothiazolinone and chlormethylisothiazolinone; three specific sulphonates and sulfamic acid, salicylic acid and its analogue sodium benzoate, propane‐1,2‐diol, glycerol, propylidynetrimethanol, lactic acid, disodium malate, morpholine, bronopol and benzyl alcohol. In conclusion, we identified an asthma induction potential for some of the substances. In addition, we identified major knowledge gaps for most substances. Thus, more data are needed to feed into a strategy of safe‐by‐design, where substances with potential for induction of asthma are avoided in future (spray) cleaning products. Moreover, we suggest that QSAR predictions can serve to prioritise substances that need further testing in various areas of toxicology.