Miracle-dz/UniFAR-FTUs huggingface dataset:
Factors influencing autumn–winter movements of midcontinent Mallards and consequences for harvest and habitat management

Abstract Annual phenology and distributions of migratory wildlife have been noticeably influenced by climate change, leading to concerns about sustainable populations. Recent studies exploring conditions influencing autumn migration departure have provided conflicting insights regarding factors influencing the movements of Mallards (Anas platyrhynchos), a popular game species. We determined factors affecting timing and magnitude of long‐distance movements of 97 juvenile Mallards during autumn‐winter across the midcontinent of North America marked with implanted transmitters in North and South Dakota, 2018–2019. Factors influencing variation in movement timing, along with direction and magnitudes, depended on type of movement (i.e., regional [25–310 km], initial migration, or subsequent migration movements [>310 km]). Photoperiod influenced probability of initiating all movements, although the effect was most influential for regional movements. Minimum temperature most influenced initial migration events (probability of movement increased 29% for each 1°C decrease); favorable winds also increased likelihood of initial migration events. Probability of subsequent migration events increased 80% for each 1 cm increase in depth of snow. Subsequent migration movements also were 2.0 times more likely to occur on weekend days, indicating disturbance from humans may influence movements. Migration distances increased 166 km for each 1°C reduction in minimum temperature. We also observed markedly different autumn‐winter distributions of marked birds between years. Median locations during autumn‐winter 2018–2019 were ~250 km farther north and ~300 km farther west during mid‐December–January compared to the same time in 2019–2020. Concurrently, harvest rates for marked females and males were 10% and 26% during autumn‐winter 2018–2019 and 26% and 31% during autumn‐winter 2019–2020. Climate‐related changes may result in increasingly variable autumn‐winter distributions, with implications for wildlife recreationalists, conservation planners, and harvest managers.
Exploring the mechanisms underlying stroke volume variability reduction in a murine model of heart failure with reduced ejection fraction

Heart failure with reduced ejection fraction (HFrEF) is accompanied by disregulation of cardiovascular function. Heart rate variability (HRV) is commonly used to assess autonomic dysfunction in HFrEF. However, analysis of stroke volume variability (SVV) may provide additional insights. We examined HRV and SVV in a mouse model of HFrEF. HFrEF mice exhibited reduced stroke volume and ejection fraction versus controls, confirming cardiac contractile dysfunction. HRV was preserved in HFrEF mice. However, SVV was markedly diminished, indicating dissociation between HRV and SVV regulation. Using a mathematical model, we propose that Frank-Starling mechanism abnormalities in HFrEF disrupt SVV independent of HRV. Assessing SVV could thus provide unique insights beyond HRV into cardiovascular control deficits in HFrEF.
Measuring the relevance and impact of innovation and social forces for Transnational Value Chain’s embeddedness in a region

This paper focuses on understanding and explaining TVC embeddedness with the theory of Regional Innovation Systems, Social Fields, and Qualitative Comparative Analysis, which allows us to show the sufficient and necessary conditions for TVC embeddedness in a region. A qualitative-comparative empirical study was conducted in 17 regions in the form of semi-structured, expert Focus Group Interviews. The participants were regional stakeholders, representatives of supportive regional institutions, businesses, and academia, accustomed to the regional innovation processes. The findings show that the strongest effect comes from the presence of the Support for Regional Innovativeness. The presence of Innovations and networks also have to be considered, meanwhile, when it comes to institutions, the results point out that the presence and absence of Institutional framework contribute to the Regional TVC Embeddedness. Lastly, we can also highlight the absence of Cognitive Frames, which are important for Regional TVC Embeddedness. The data presented in the paper does not separate the TIER levels. Different levels could influence the conditions for the embeddedness. Half-products that are closer to the end product tend to have higher added value and are more innovative. Based on a newly developed theory and model, which focuses on social field theory and innovation, the present paper aims to test them in real-life settings. This model explains how the social forces and innovation processes influence the company embeddedness by emphasising the necessary and sufficient conditions to have TVC embeddedness.
Context-specific synthetic T cell promoters from assembled transcriptional elements

Abstract Genetic engineering of human lymphocytes for therapeutic applications is constrained by a lack of transgene transcriptional control, resulting in a compromised therapeutic index. Incomplete understanding of transcriptional logic limits the rational design of contextually responsive genetic modules 1 . Here, we juxtaposed rationally curated transcriptional response element (TRE) oligonucleotides by random concatemerization to generate a library from which we selected context-specific inducible synthetic promoters (iSynPros). Through functional selection, we screened an iSynPro library for “IF-THEN” logic-gated transcriptional responses in human CD8 + T cells expressing a 4-1BB second generation chimeric antigen receptor (CAR). iSynPros exhibiting stringent off-states in quiescent T cells and CAR activation-dependent transcriptional responsiveness were cloned and subjected to TRE composition and pattern analysis, as well as performance in regulating candidate antitumor potency enhancement modules. These data reveal synthetic TRE grammar can mediate logic-gated transgene transcription in human T cells that, when applied to CAR T cell engineering, enhance potency and improve therapeutic indices.
Differential Pulmonary Toxicity and Autoantibody Formation in Genetically Distinct Mouse Strains Following Combined Exposure to Silica and Diesel Exhaust Particles

Background Inhalation of airborne particulate matter, such as silica and diesel exhaust particles, poses serious long-term respiratory health risks. Silica exposure can lead to silicosis and systemic autoimmune diseases, while DEP exposure is linked to asthma and cancer. Combined exposure to silica and DEP, common in mining, may have more severe effects. This study investigates the separate and combined effects of silica and DEP on lung injury, inflammation, and autoantibody formation in two genetically distinct mouse strains, thereby aiming at understanding the interplay between genetic susceptibility, particulate exposure, and disease outcomes. Silica and diesel exhaust particles were administered to mice via oropharyngeal aspiration. Assessments of lung injury and host response included in vivo lung micro-computed tomography, lung function tests, bronchoalveolar lavage fluid analysis including inflammatory cytokines and antinuclear antibodies, and histopathology with particle colocalization. Results Silica exposure elicited a well-established inflammatory response marked by inflammatory infiltrates, release of cytokines, and chemokines, alongside limited fibrosis, indicated by collagen deposition in the lungs of both C57BL/6J and NOD/ShilLtJ mice. Notably, these strains exhibited divergent responses in terms of respiratory function and lung volumes, as assessed through micro-computed tomography. Additionally, silica exposure induced airway hyperreactivity and elevated antinuclear antibody levels in bronchoalveolar lavage fluid, particularly prominent in NOD/ShiLtJ mice. Lung tissue analysis revealed DEP loaded macrophages and co-localization of silica and DEP particles. Conclusion Mouse strain variations exerted a substantial influence on the development of silica induced lung alterations. Furthermore, the additional impact of diesel exhaust particles on these silica-induced effects was minimal.
Assessment on heavy metals pollution in surface sediments in Jinzhou Bay

The contents of 6 heavy metal elements in sediments in Jinzhou Bay were analyzed by a mass spectrometry with inductive coupled plasma(ICP-MS). The potential ecological risk index(Hkanson) was employed for the assessment of the sediment quality.The results showed that the pollution of Zn,As,Cd and Pb was very serious in the surface sediments,and the potential ecological risk of trace metals was very high in Jinzhou Bay.
Acute kidney injury following treatment with CD19-specific CAR T-cell therapy in children, adolescent and young adult patients with B-cell acute lymphoblastic leukemia

CD19-specific chimeric antigen receptor (CAR) T-cell therapy has shown promising disease responses in patients with high-risk B-cell malignancies. Treatment with CD19-CAR T-cell therapy is also associated with the risk of morbidity and mortality, primarily related to immune-mediated complications (cytokine release syndrome [CRS] and neurotoxicity [NTX]), infections, and end-organ dysfunction. Despite these well-described systemic toxicities, the incidence of post-CAR T-cell therapy acute kidney injury (AKI) in the children, adolescent and young adult (CAYA) patient population is largely unreported. The objectives of this study were to determine the incidence of AKI in CAYA patients with high-risk B-cell malignancies treated with CD19-CAR T-cell therapy, evaluate potential risk factors for developing AKI, and determine patterns of kidney function recovery. In this retrospective analysis of 34 CAYA patients treated with CD19-CAR T-cell at a single institution, we found a cumulative incidence of any grade AKI by day 30 post-infusion of 20% (n=7), with 4 cases being severe AKI (Stage 2–3) and one patient requiring kidney replacement therapy. All episodes of AKI developed within the first 14 days after receiving CAR T-cell therapy and 50% of patients with AKI recovered kidney function to baseline within 30 days post-infusion. No evaluated pre-treatment risk factors were associated with the development of subsequent AKI; there was an association between AKI and CRS and NTX. We conclude that the risk of developing AKI following CD19-CAR T-cell therapy is highest early post-infusion, with most cases of AKI being severe. Although most patients with AKI in our cohort had recovery of kidney function, frequent monitoring to facilitate early recognition and subsequent management of kidney complications after CD19-CAR T-cell therapy may reduce the severity of AKI in the CAYA patient population.
Nutrient losses under simulated rainfall from pasture plots in the Great Lakes District, New South Wales

Understanding the process by which nutrients and solids enter waterways from pastures in the Great Lakes district, New South Wales, Australia, may assist in maintaining water quality to ensure ongoing environmental and economic sustainability of the region. Rainfall simulations, using a 100-year return storm event, were conducted to determine nutrient and suspended solid concentrations in the runoff of 8 pasture sites in 3 of the catchments in the district. On 5 of the 8 sites, considerable concentrations of N or P were mobilised during the simulated rainfall event, but average nutrient concentrations and total loads across all sites were relatively low and similar to other studies of nutrient runoff from pastures. In addition, low runoff coefficients indicated that runoff is probably not the major pathway for nutrient losses from pasture in this area. Overall, rainfall runoff responses at the sites were similar in the 3 catchments. In contrast, the results suggest that, despite generating more runoff, the sites in the Wang Wauk catchment generated less nutrients in runoff than the sites in the Wallamba and Myall catchments. There was no difference in total suspended solids loads for the sites analysed by catchment. Relationships between soil physical and chemical characteristics and total nutrients loads or cumulative runoff were not strong.
Protective effect of citronellol in rhabdomyolysis-induced acute kidney injury in mice

Acute kidney injury (AKI) is a serious pathophysiological event consequent to rhabdomyolysis. Inflammatory mechanisms play a role in the development of rhabdomyolysis-induced AKI. Citronellol (CT) is a naturally occurring monoterpene in essential oils of aromatic plant species. In this study, we explored the protective effects of citronellol on AKI resulting from glycerol-induced rhabdomyolysis. Rhabdomyolysis was induced by a single intramuscular injection of glycerol 50% (10mg/kg) in the thigh caudal muscle. Four groups of mice were assigned, including a control group, a group administered with glycerol to induce AKI as a model, a group treated with glycerol plus 50mg/kg CT, and a group treated with glycerol plus 100mg/kg CT. The renal function of mice from all groups was evaluated using kidney histopathological changes and kidney injury molecule-1 (KIM-1). Myoglobin levels were measured to detect rhabdomyolysis. Apoptosis was evaluated by renal cleaved caspase-3 and BAX levels. Both doses of citronellol (50mg/kg and 100mg/kg) significantly reduced KIM-1 mRNA expression and myoglobin levels compared to the glycerol group. In addition, citronellol resulted in lower cleaved caspase-3 and BAX in the renal tissue, indicating that citronellol exerted an anti-apoptotic effect in AKI. Citronellol showed a reno-protective effect against rhabdomyolysis-induced AKI, which may be attributed to its anti-apoptotic effects.
Calredoxin regulates the chloroplast NADPH-dependent thioredoxin reductase in Chlamydomonas reinhardtii

Calredoxin (CRX) is a calcium (Ca2+)-dependent thioredoxin (TRX) in the chloroplast of Chlamydomonas reinhardtii with largely unclear physiological role. We elucidated the CRX functionality by performing in-depth quantitative proteomics of wild type cells in comparison with crx insertional mutant (IMcrx), two CRISPR/Cas9 KO mutants and CRX rescues. These analyses revealed that the chloroplast NADPH-dependent TRX reductase (NTRC) is co-regulated with CRX. Electron transfer measurements revealed that CRX inhibits NADPH-dependent reduction of oxidized chloroplast 2-Cys peroxiredoxin (PRX1) via NTRC and that the function of the NADPH-NTRC complex is under strict control of CRX. Via non-reducing SDS-PAGE assays and mass spectrometry, our data also demonstrated that PRX1 is more oxidized under high light (HL) conditions in the absence of CRX. The redox tuning of PRX1 and control of the NADPH-NTRC complex via CRX interconnects redox control with active photosynthetic electron transport and metabolism as well as Ca2+ signaling. In this way, an economic use of NADPH for PRX1 reduction is ensured. The finding, that the absence of CRX under HL conditions severely inhibited light-driven CO2 fixation underpins the importance of CRX for redox tuning as well as for efficient photosynthesis. One-sentence summary Calredoxin dependent redox regulation ensures efficient photosynthesis.
Butyrate protects and synergizes with nicotine against iron- and manganese-induced toxicities in cell culture: Implications for neurodegenerative diseases

Toxic exposures to heavy metals, such as iron (Fe) and manganese (Mn), can result in long-range neurological diseases and are therefore of significant environmental and medical concerns. We have previously reported that damage to neuroblastoma-derived dopaminergic cells (SH-SY5Y) by both Fe and Mn could be prevented by pre-treatment with nicotine. Moreover, butyrate, a short chain fatty acid (SCFA) provided protection against salsolinol, a selective dopaminergic toxin, in the same cell line. Here, we broadened the investigation to determine whether butyrate might also protect against Fe and/or Mn, and whether, if combined with nicotine, an additive or synergistic effect might be observed. Both butyrate and nicotine concentration-dependently blocked Fe and Mn toxicities. The ineffective concentrations of nicotine and butyrate, when combined, provided full protection against both Fe and Mn. Moreover, the effects of nicotine but not butyrate could be blocked by mecamylamine, a non-selective nicotinic antagonist. On the other hand, the effects of butyrate, but not nicotine, could be blocked by beta-hydroxy butyrate, a fatty acid-3 receptor antagonist. These results not only provide further support for neuroprotective effects of both nicotine and butyrate but indicate distinct mechanisms of action for each one. Furthermore, potential utility of the combination of butyrate and nicotine against heavy metal toxicities is suggested.
Enhanced wound healing and hemostasis with exosome-loaded gelatin sponges from human umbilical cord mesenchymal stem cells

BACKGROUND Rapid wound healing remains a pressing clinical challenge, necessitating studies to hasten this process. A promising approach involves the utilization of human umbilical cord mesenchymal stem cells (hUC-MSCs) derived exosomes. The hypothesis of this study was that these exosomes, when loaded onto a gelatin sponge, a common hemostatic material, would enhance hemostasis and accelerate wound healing. AIM To investigate the hemostatic and wound healing efficacy of gelatin sponges loaded with hUC-MSCs-derived exosomes. METHODS Ultracentrifugation was used to extract exosomes from hUC-MSCs. Nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blot techniques were used to validate the exosomes. In vitro experiments were performed using L929 cells to evaluate the cytotoxicity of the exosomes and their impact on cell growth and survival. New Zealand rabbits were used for skin irritation experiments to assess whether they caused adverse skin reactions. Hemolysis test was conducted using a 2% rabbit red blood cell suspension to detect whether they caused hemolysis. Moreover, in vivo experiments were carried out by implanting a gelatin sponge loaded with exosomes subcutaneously in Sprague-Dawley (SD) rats to perform biocompatibility tests. In addition, coagulation index test was conducted to evaluate their impact on blood coagulation. Meanwhile, SD rat liver defect hemostasis model and full-thickness skin defect model were used to study whether the gelatin sponge loaded with exosomes effectively stopped bleeding and promoted wound healing. RESULTS The NTA, TEM, and western blot experimental results confirmed that exosomes were successfully isolated from hUC-MSCs. The gelatin sponge loaded with exosomes did not exhibit significant cell toxicity, skin irritation, or hemolysis, and they demonstrated good compatibility in SD rats. Additionally, the effectiveness of the gelatin sponge loaded with exosomes in hemostasis and wound healing was validated. The results of the coagulation index experiment indicated that the gelatin sponge loaded with exosomes had significantly better coagulation effect compared to the regular gelatin sponge, and they showed excellent hemostatic performance in a liver defect hemostasis model. Finally, the full-thickness skin defect healing experiment results showed significant improvement in the healing process of wounds treated with the gelatin sponge loaded with exosomes compared to other groups. CONCLUSION Collectively, the gelatin sponge loaded with hUC-MSCs-derived exosomes is safe and efficacious for promoting hemostasis and accelerating wound healing, warranting further clinical application.
Chapter 4. Borneo as a Cross-Roads for Comparative Austronesian Linguistics

The autochthonous languages of Borneo have been divided into ten separate subgroups (Hudson 1978). This paper discusses four subgroups on which the author has done research. The Southeast Barito subgroup includes Malagasy. This language underwent considerable influence from Malay and Javanese. Malay influence appears to have lasted until after the introduction of Islam in Southeast Asia, and there are also some indications that the Arabic script was introduced to Madagascar by Indonesians (possibly Javanese). The author puts forth the hypothesis that the Malagasy, rather than having sailed to Madagascar of their own accord, may have been transported there (as subordinates) by Malays. The Malayic subgroup includes Iban and Malay. The diversity and relative archaism of the Malayic languages spoken in West Borneo suggest that the Malayic homeland may have been in this area. The Tamanic languages are phonologically, morphosyntacti cally and lexically close enough to the South Sulawesi languages to form a subgroup with them. They have some striking phonological developments in common with Buginese, with which they seem to form a separate branch within the South Sulawesi language group. The Land Dayak languages have a f ew str iking lexical and phonological similarities in common with Aslian languages. This suggests that Land Dayak originated as the result of a language shift from Aslian to Austronesian, or that both Land Dayak and Aslian have in common a substratum from an unknown third language.
Challenges and considerations for treating PTSD with medicinal cannabis: the Australian clinician’s perspective

ABSTRACT Introduction Preclinical and experimental research have provided promising evidence that medicinal cannabis may be efficacious in the treatment of posttraumatic stress disorder (PTSD). However, implementation of medicinal cannabis into routine clinical therapies may not be straightforward. Areas covered In this review, we describe some of the clinical, practical, and safety challenges that must be addressed for cannabis-based treatment of PTSD to be feasible in a real-world setting. These issues are especially prevalent if medicinal cannabis is to be combined with trauma-focused psychotherapy. Expert opinion Future consideration of the clinical and practical considerations of cannabis use in PTSD therapy will be essential to both the efficacy and safety of the treatment protocols that are being developed. These issues include dose timing and titration, potential for addiction, product formulation, windows of intervention, and route of administration. In particular, exposure therapy for PTSD involves recall of intense emotions, and the interaction between cannabis use and reliving of trauma memories must be explored in terms of patient safety and impact on therapeutic outcomes.
The Over-Urbanization and Social Stability in the Middle East

Urbanization is a path to socioeconomic development for various countries of the world.However,after the Word War II,the Middle Eastern countries have formed their own model which is different from their developed Western counterparts.The formers have been implementing the Emulation Strategy of urbanization,with development of urban as their priority in policy-making thus causing over-urbanization.So far,the over-urbanization have brought about a series of social problems and exerted serious negative influence upon the social stability in the Middle East.