Miracle-dz/UniFAR-FTUs huggingface dataset:
ATX-LPA-Dependent Nuclear Translocation of Endonuclease G in Respiratory Epithelial Cells: A New Mode Action for DNA Damage Induced by Crystalline Silica Particles

Simple Summary Crystalline silica particles (CSi) are carcinogenic; however, their genotoxicity is not well characterized. Indirect evidence suggests that retained particles in peripheral bronchioles activate macrophages, causing chronic ROS production. Using human bronchial epithelia in monocultures, we have recently shown that CSi activate ATX at the plasma membrane and that a complex signaling pathway leads to double strand breaks (DSBs). The DSBs were seen within minutes, also in mice inhaling CSi. A remaining question is how DSBs are induced. Using the same models as previously, we now show that ATX signaling leads to endonuclease G translocation from the mitochondria to the nucleus. It was synchronized with DSB formation, and inhibitors prevented both the translocation and the DSBs, supporting a causal role for endonuclease G. The CSi-induced micronuclei formation indicated genomic instability. Additionally, the endonuclease G mediated genotoxicity, which thus affects the respiratory epithelium directly at very low particle doses, might explain the carcinogenic effects of CSi, perhaps even better than ROS-producing macrophages. Abstract Crystalline silica particles (CSi) are an established human carcinogen, but it is not clear how these particles cause necessary mutations. A well-established scenario includes inflammation caused by retained particles in the bronchioles, activated macrophages, and reactive oxygen species (ROS) that cause DNA damage. In previous studies, we showed that CSi in contact with the plasma membrane of human bronchial epithelium induced double strand breaks within minutes. A signaling pathway implicating the ATX-LPA axis, Rac1, NLRP3, and mitochondrial depolarization upstream of DSB formation was delineated. In this paper, we provide in vitro and in vivo evidence that this signaling pathway triggers endonuclease G (EndoG) translocation from the mitochondria to the nucleus. The DNA damage is documented as γH2AX and p53BP1 nuclear foci, strand breaks in the Comet assay, and as micronuclei. In addition, the DNA damage is induced by low doses of CSi that do not induce apoptosis. By inhibiting the ATX-LPA axis or by EndoG knockdown, we prevent EndoG translocation and DSB formation. Our data indicate that CSi in low doses induces DSBs by sub-apoptotic activation of EndoG, adding CSi to a list of carcinogens that may induce mutations via sub-apoptotic and “minority MOMP” effects. This is the first report linking the ATX-LPA axis to this type of carcinogenic effect.
Design of 8:1 Multiplexer Using Gate Diffusion Input (GDI) Technique and Comparison of Delay Performance with Pass Transistor Logic

Lot of advancement in VLSI technology makes low power and low energy as important issues in consumer electronics. In digital circuits, multiplexer or data selector plays an important role where it processes multiple input lines and gives a single output. With regard to pass transistor logic, the paper proposes to construct an 8:1 multiplexer using the Innovative Gate Diffusion Input (GDI) technique (PTL). In this paper, the multiplexer is designed using basic GDI cell. The design of digital combinational circuit by GDI technique is done using Tanner EDA tool and DSCH3. The GDI technique overcomes the disadvantages in Pass Transistor Logic(PTL). These logic styles are simulated, and To compare the propagation delay, power, and transistor count, a total of 20 samples are employed. The pass transistor logic has a propagation delay of 0.06378ns and a power dissipation of 0.06191 w, compared to the GDI technique's propagation delay and power dissipation of 0.04759ns and 0.04007w, respectively. Innovative GDI technique gives less propagation delay and power consumption when compared with pass transistor logic.
Developing and Extending Status Prediction Models for Railway Tracks Based on On-Board Monitoring Data

Assigning inspection trains to monitor track quality is a standard procedure for maintaining railway system safety. The main challenges lie in lacking time and resources to perform the inspections because of the increasing traffic nowadays. To overcome these challenges, many consider adopting the on-board monitoring (OBM) technique for performing the inspections. This technique assigns commercial trains, instead of traditional track recording vehicles (TRVs), to monitor the track status, allowing railway operators to perform more inspections without affecting the traffic and using expensive inspection trains as well. However, compared with TRV data, the new OBM data are of lower data quality and have fewer features, although they can be recorded more frequently. Therefore, new methods should be developed for effectively applying the new data. This study develops four models, namely the linear regression model, Markov model, ordinary Kriging model, and Kalman filter model, for predicting the track status based on the OBM data. Data collected from the Switzerland railway network are used for verifying the models. Results show that the proposed models can effectively predict the degradation of the track status in different ways and, therefore, assist railway operators in scheduling maintenance tasks.
Erbium-ytterbium co-doped fiber amplifier with controlled 1060-nm Yb-ASE

In this work we present a new design of Er/Yb co-doped fiber amplifier with controlled amplified spontaneous emission (ASE) at 1 μm. The Yb-ASE is controlled by stimulating a laser emission at 1064 nm in the amplifier, by providing a positive 1 μm signal feedback loop. The results were discussed and compared to a conventional amplifier setup without 1 μm ASE control. We have shown, that applying a 1064 nm signal loop in an Er/Yb amplifier can increase the output power at 1550 nm and provide stable operation without parasitic lasing at 1 μm.
Conquering Noise With Hardware Counters on HPC Systems

With increasing system performance and complexity, it is becoming increasingly crucial to examine the scaling behavior of an application and thus determine performance bottlenecks at early stages. Unfortunately, modeling this trend is a challenging task in the presence of noise, as the measurements can become irreproducible and misleading, thus resulting in strong deviations from the actual behavior. While noise impacts the application runtime, it has little to no effect on some hardware counters like floating-point operations. However, selecting the appropriate counters for performance modeling demands some investigation. In this paper, we perform a noise analysis on various hardware counters. Using our noise generator, we add additional noise on top of the system noise to inspect the counters' variability. We perform the analysis on five systems with three applications in the presence of various noise patterns and categorize the counters across the systems according to their noise resilience.
PHYSICS AND TACTILE PERCEPTION OF FLUID‐COVERED SURFACES

Little is known about the linkage between the physical properties of fluids and how they are perceived. Therefore, 15 fluids with diverse rheologies were characterized physically by three participants via a novel “force plate” (FP) device. Perceptual ratings were made by 20 participants, of 27 “sensory” attributes (e.g., oily) and 14 “emotional” attributes (e.g., arousing). Factor analyses decomposed 14 physical measures obtained from the FP into factors approximating friction, normal vibration, and tangential vibration; the sensory attributes into watery, textured, slick, silken and viscous factors; and the emotional attributes into factors labeled pleasant, sensual and harsh. Pleasant and harsh were related to textured and silken. Fluids perceived as more watery elicited greater normal and tangential vibration and less friction, whereas high viscous scores were associated with lower vibration factor scores and increasing friction. These results emphasize the complex perceptual nature of fluid-coated surfaces as compared with dry, solid surfaces. PRACTICAL APPLICATIONS In developing consumer products that are fluid-based (e.g., various foods or cosmetic preparations), it is desirable to reach an end goal in terms of how the fluid feels. This is achieved primarily by altering the physical composition of the fluid. The reported research established relationships between the percepts and physical composition of a diverse set of fluids. These relationships allow early insights concerning how one might formulate a product to produce a given, desirable, percept.
A Compact SIW Bandpass Filter Embedded with Folded Quarter-Wavelength Resonators

A novel single-layer bandpass filter is proposed by combining two substrate integrated waveguide (SIW) cavities and two folded quarter-wavelength microstrip line resonators, which results in a 4th-order filtering response. By introducing the cross-coupling, two transmission zeros (TZs) are generated to ensure the great selectivity of the proposed filter. Besides, adjustment of the feeding position gives rise to wide stopband rejection. The practical hybrid structure is built on Rogers 5880 sub-strate (0.508mm). The measured and simulated S-parameters of the filter show a series of advantages such as miniaturized size, low insertion loss, high selectivity and high out-of-band rejection, although some discrepancy is observed in fabrication which deteriorates the spurious mode rejection.
On the differential rotation of CoRoT-2a

We apply a robust spot model to fit the light curve of CoRoT-2a. The spots are assumed to be long-living and each has its own rotation period. A model with three circular spots reproduces the basic features of the longitude-time spot coverage map. One of the spots exhibits a noticeably lower rotational frequency than the other two. From the rotational frequencies of the three dark features we infer ad ifferential rotation of above 0.11 rad/d, in rough agreement with theoretical models. Mean field models of angular momentum transport by convection and meridional flow lead to an equatorial rotational frequency that exceeds that of the poles by 0.09 rad/d. The spot decay corresponds to a turbulent magnetic diffusivity of (1.2 ± 0.1) × 10 13 cm 2 /s.
Evaluation of Clinical Characteristics and CT Decision Rules in Elderly Patients with Minor Head Injury: A Prospective Multicenter Cohort Study

Age is variably described as a minor or major risk factor for traumatic intracranial lesions after head injury. However, at present, no specific CT decision rule is available for elderly patients with minor head injury (MHI). The aims of this prospective multicenter cohort study were to assess the performance of existing CT decision rules for elderly MHI patients and to compare the clinical and CT characteristics of elderly patients with the younger MHI population. Thirty-day mortality between two age groups (cutoff ≥ 60 years), along with clinical and CT characteristics, was evaluated with four CT decision rules: the National Institute for Health and Care Excellence (NICE) guideline, the Canadian CT Head Rule (CCHR), the New Orleans Criteria (NOC), and the CT Head Injury Patients (CHIP) rule. Of the 5517 MHI patients included, 2310 were aged ≥ 60 years. Elderly patients experienced loss of consciousness (17% vs. 32%) and posttraumatic amnesia (23% vs. 31%) less often, but intracranial lesions (13% vs. 10%), neurological deterioration (1.8% vs. 0.2%), and 30-day mortality (2.0% vs. 0.1%) were more frequent than in younger patients (all p < 0.001). Elderly patients with age as their only risk factor showed intracranial lesions in 5% (NOC and CHIP) to 8% (CCHR and NICE) of cases. The sensitivity of decision rules in the elderly patients was 60% (CCHR) to 97% (NOC) when age was excluded as a risk factor. Current risk factors considered when evaluating elderly patients show lower sensitivity to identify intracranial abnormalities, despite more frequent intracranial lesions. Until age-specific CT decision rules are developed, it is advisable to scan every elderly patient with an MHI.
Factors Affecting Continuance Intention in Non-Face-to-Face Telemedicine Services: Trust Typology and Privacy Concern Perspectives

As the COVID-19 pandemic progressed, the resulting demand for telemedicine services increased. This research empirically examines the role of trust, privacy concerns, and perceived usefulness in customer confirmation, satisfaction, and continuing intention in telemedicine. A typology of trust was employed to classify trust into three dimensions and explore the mediating role of the three dimensions of trust in the relationship between satisfaction, perceived usefulness, and continued intention. We also examined the moderating role of personal privacy concerns in the relationship between trust and continued intention. For this study, we developed a structural equation model based on expectation confirmation theory and analyzed 465 questionnaires from Chinese online users. The expectancy confirmation theory (ECT) was reaffirmed by empirical evidence. The results showed that the relationship between perceived usefulness and satisfaction with continued intention is moderated by the three dimensions of trust. Privacy concerns can negatively moderate the relationship between structural assurance-based trust and continued intention. This study also identified potential threats to telehealth market growth alongside new insights.
Y-27632 Impairs Angiogenesis on Extra-Embryonic Vasculature in Post-Gastrulation Chick Embryos

Y-27632 inhibits Rho-associated coiled-coil-containing protein kinase (ROCK) signaling, which is involved in various embryonic developmental processes, including angiogenesis, by controlling actin cytoskeleton assembly and cell contractility. Administration of Y-27632 impairs cytoskeletal arrangements in post-gastrulation chick embryos, leading to ventral body wall defects (VBWDs). Impaired angiogenesis has been hypothesized to contribute to VBWDs. ROCK is essential in transmitting signals downstream of vascular endothelial growth factor (VEGF). VEGF-mediated angiogenesis induces gene expressions and alterations of the actin cytoskeleton upon binding to VEGF receptors (VEGFRs). The aim of this study was to investigate effects of Y-27632 on angiogenesis in post-gastrulation chick embryos during early embryogenesis. After 60 h incubation, embryos in shell-less culture were treated with Y-27632 or vehicle for controls. Y-27632-treated embryos showed reduced extra-embryonic blood vessel formation with impaired circulation of the yolk sac, confirmed by fractal analysis. Western blot confirmed impaired ROCK downstream signaling by decreased expression of phosphorylated myosin light chain. Interestingly, RT-PCR demonstrated increased gene expression of VEGF and VEGFR-2 1 h post-treatment. Protein levels of VEGF were higher in Y-27632-treated embryos at 8 h following treatment, whereas no difference was seen in membranes. We hypothesize that administration of Y-27632 impairs vessel formation during angiogenesis, which may contribute to failure of VWB closure, causing VBWDs.
Prediction of channel freedom limit by parabolic equation method

In a communication system, the effective degree of freedom (DoF) has a limit with the increasing of the number of sources/receivers. In this paper, the parabolic equation method is used to establish the electromagnetic (EM) channel model. And the correctness of the model is verified by comparison with the free space analytical solution. In addition, the sensitivity between the size of the transmitting surface and the effective degree of freedom is analyzed by using parabola method. The channel matrix model based on the parabolic algorithm provides a reference for establishing efficient and stable communication lines in free space.
Research on forecast and suppression splashing of AOD refining process

During the smelting process of the AOD furnace, the unbalanced reaction of material will lead to the occurrence of splashing. It will not only damage the smelting equipment but also seriously injure the personnel. In this study, first, the information of liquid level, audio information and vibration information are detected by multiple sensors respectively. Then the fused information is used to forecast the splashing. Finally, the multitasking fuzzy controller is used to suppress splashing. The results show that the method of forecasting and suppressing splashing can accurately forecast and achieve rapid suppression. Thus the efficiency of smelting can be improved.
Source Apportionment and Health Risk Assessment of Heavy Metals in Soils of Old Industrial Areas—A Case Study of Shanghai, China

Heavy metals in the soil of industrial areas pose severe health risks to humans after land-use properties are transformed into residential land. The public exposure time and frequency will soar significantly under residential land. However, much uncertainty still exists about the relationship between soil heavy metal pollution and—human health risks in an old industrial zone in Shanghai, China. Principal component analysis—(PCA) was used to explore the main sources of these heavy metals. Kriging interpolation was u-sed to identify their spatial distribution and high-risk areas, and the Human Health risk model was used to measure health risk. The results illustrate that the pollution levels of Cd, Hg, and Pb in industrial land are more serious than those in irrigation cropland. Meanwhile, the results of PCA showed that there were two main pollution sources under irrigated cropland, a natural source and a traffic source, accounting for 44.1% and 31.0%, respectively, and there were three main pollution sources under industrial land, with natural sources accounting for 28.5%, traffic sources accounting for 25.7%, and industrial sources accounting for 13.1%. In addition, the health risk assessment results indicated that the priority control pollutants of non-carcinogenic risk and carcinogenic risk were Zn and Cr, respectively. The high-risk area was mainly located in the middle of the study area. These results indicate that eliminating heavy metal pollution in the soil of the industrial area is so important to decrease health risks. The results of this study provide theoretical contributions to early warning of health risks related to heavy metal pollution in industrial area soil and serve as a practical reference for speeding up the formulation of industrial land pollution management policies.
Pulse-Voltage-Based Method of Thermal Parameter Extraction for Thermopile Infrared Sensors

Thermopile infrared (IR) sensors based on the thermoelectric effect are widely applied in industrial and civil fields. Researchers urgently need to develop a technique for in situ online extraction of the sensor’s thermal parameters, in order to allow for better analysis of the sensor’s performance and to improve its structure. In this work, a pulse-voltage-based self-test method is proposed. The pulse voltage can be generated easily, and only a few data points are required, with simple data processing. The measured results are close to the theoretical values and results from the constant current method proposed in another paper, thus indicating that our method is effective and accurate. In addition, it has strong significance for array testing.
LHC-like Proteins: The Guardians of Photosynthesis

The emergence of chlorophyll-containing light-harvesting complexes (LHCs) was a crucial milestone in the evolution of photosynthetic eukaryotic organisms. Light-harvesting chlorophyll-binding proteins form complexes in proximity to the reaction centres of photosystems I and II and serve as an antenna, funnelling the harvested light energy towards the reaction centres, facilitating photochemical quenching, thereby optimizing photosynthesis. It is now generally accepted that the LHC proteins evolved from LHC-like proteins, a diverse family of proteins containing up to four transmembrane helices. Interestingly, LHC-like proteins do not participate in light harvesting to elevate photosynthesis activity under low light. Instead, they protect the photosystems by dissipating excess energy and taking part in non-photochemical quenching processes. Although there is evidence that LHC-like proteins are crucial factors of photoprotection, the roles of only a few of them, mainly the stress-related psbS and lhcSR, are well described. Here, we summarize the knowledge gained regarding the evolution and function of the various LHC-like proteins, with emphasis on those strongly related to photoprotection. We further suggest LHC-like proteins as candidates for improving photosynthesis in significant food crops and discuss future directions in their research.
A code for N-body simulation of highly clustered gravitational systems

Un systeme de coordonnees spheriques peut etre transforme en un demi-tore 3D par une cartographie trigonometrique du domaine radial. Les variables angulaires etant les seules impliquees, les harmoniques spheriques et les expansions en cosinus des variables du champ peuvent etre utilises. En suivant cette approche, un algorithme N-corps base sur la technique Particle-Mesh est realise pour simuler les systemes gravitants, sans collisions, et fortement heterogenes. Les performances et la fiabilite numerique sont testees pour quelques configurations d'equilibre stable et instable.
BET Bromodomain Degradation Disrupts Function but Not 3D Formation of RNA Pol2 Clusters

Fusion-positive rhabdomyosarcoma (FP-RMS) is driven by a translocation that creates the chimeric transcription factor PAX3-FOXO1 (P3F), which assembles de novo super enhancers to drive high levels of transcription of other core regulatory transcription factors (CRTFs). P3F recruits co-regulatory factors to super enhancers such as BRD4, which recognizes acetylated lysines via BET bromodomains. In this study, we demonstrate that inhibition or degradation of BRD4 leads to global decreases in transcription, and selective downregulation of CRTFs. We also show that the BRD4 degrader ARV-771 halts transcription while preserving RNA Polymerase II (Pol2) loops between super enhancers and their target genes, and causes the removal of Pol2 only past the transcriptional end site of CRTF genes, suggesting a novel effect of BRD4 on Pol2 looping. We finally test the most potent molecule, inhibitor BMS-986158, in an orthotopic PDX mouse model of FP-RMS with additional high-risk mutations, and find that it is well tolerated in vivo and leads to an average decrease in tumor size. This effort represents a partnership with an FP-RMS patient and family advocates to make preclinical data rapidly accessible to the family, and to generate data to inform future patients who develop this disease.
Development of conducting paper-based electrochemical biosensor for procalcitonin detection

In the present research, an advanced cellulose fiber paper (CFP) based biosensor is developed. This sensor is modified with nanocomposites containing poly(3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) as the main matrix and functionalized gold nanoparticles (PEDOT:PSS-AuNP@CFP) for the selective and sensitive detection of bacterial infection (BI)-specific biomarker procalcitonin (PCT). Scanning electronic microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction are used to characterize the PEDOT:PSS-AuNP nanocomposite. This biosensor exhibits a high sensitivity of 1.34 μA (pg mL-1)-1 in the linear detection ranges of 1-20×104 pg mL-1, and a 24-day life span for PCT antigen detection. Anti-PCT antigenic protein is used for immobilization for PCT quantification. The results of electrochemical response studies showed that this conductive paper bioelectrode had good reproducibility, stability, and sensitivity in physiological ranges (1-20×104 pg mL-1). Further, the proposed bioelectrode is an alternative choice for point-of-care PCT detection.