Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of mammalian target of rapamycin and aryl hydrocarbon receptor‐mediating autophagy signaling on the balance of Th17/Treg cells during perinatal bisphenol A exposure in female offspring mice

Bisphenol A (BPA)'s immunotoxic properties have received increasing interest, which can lead to immune dysfunction and related disease development. However, the mechanism is not completely clear. A growing body of evidence suggests that autophagy has important roles in innate immunity, inflammatory response, and adaptive immunity. This study aimed to investigate the possible mechanisms of mammalian target of rapamycin (mTOR), aryl hydrocarbon receptor (AhR), and autophagy in Treg/Th17 imbalance induced by perinatal BPA exposure. Our results showed that the number of Th17 cells in the spleen of offspring female mice significantly increased, while the number of Treg cells decreased significantly, which was consistent with the expression levels of up‐regulation of RORγt protein and a down‐regulation Foxp3 protein. The levels of mTOR, p‐mTOR, P62, and AhR protein expression increased, and LC3 protein decreased in spleen. However, in the thymus, we found that RORγt and Foxp3 proteins changed most significantly in the low‐dose BPA group, and the same as p‐mTOR and P62 protein levels. We conjectured that the potential mechanism of the imbalance of Th17/Treg upon perinatal exposure to BPA was probably associated with autophagy dysfunction. Proper autophagy plays an important role in maintaining the homeostasis of the thymic and spleen immune system.
Photoprotection conferring plant tolerance to freezing stress through rescuing photosystem in evergreen Rhododendron.

Light stress is one of the important stresses for winter survival in evergreens, especially for plants with broad leaves, like evergreen rhododendrons. Photoprotection has been shown to upregulate dramatically in rhododendrons during winter, but whether it directly contributes to enhancing the freezing tolerance is still unknown. In this study, we found that the expression and circadian rhythm of an Early Light-Induced Protein-RhELIP3, which exerts photoprotection in Rhododendron 'Elsie Lee', could be impacted by both photoperiod and low temperature, with low temperature being the predominant inducer. Arabidopsis overexpressing RhELIP3 displayed significant stronger freezing tolerance and better photosystem II functioning after 3-day recovery from freezing treatment. Moreover, RhHY5 binds with RhELIP3 promoter to active its expression. Arabidopsis overexpressing RhHY5 exhibited stronger freezing tolerance and better photosystem II functioning. AtELIP1 and AtELIP2 were significantly induced in RhHY5-overexpressed Arabidopsis at low temperature. We also discovered that RhBBX24 binds directly to RhELIP3 promoter and suppresses its expression. RhBBX24 can also interact with RhHY5 and inhibit the interaction of RhHY5-RhELIP3. RhELIP3, RhHY5, and RhBBX24 exhibited similar circadian rhythms under the low temperature with short photoperiod. Overall, our investigation highlights that photoprotection is involved in improving the freezing tolerance of evergreen rhododendrons. This article is protected by copyright. All rights reserved.
Key biological mechanisms involved in high-LET radiation therapies with a focus on DNA damage and repair

Abstract DNA damage and repair studies are at the core of the radiation biology field and represent also the fundamental principles informing radiation therapy (RT). DNA damage levels are a function of radiation dose, whereas the type of damage and biological effects such as DNA damage complexity, depend on radiation quality that is linear energy transfer (LET). Both levels and types of DNA damage determine cell fate, which can include necrosis, apoptosis, senescence or autophagy. Herein, we present an overview of current RT modalities in the light of DNA damage and repair with emphasis on medium to high-LET radiation. Proton radiation is discussed along with its new adaptation of FLASH RT. RT based on α-particles includes brachytherapy and nuclear-RT, that is proton-boron capture therapy (PBCT) and boron-neutron capture therapy (BNCT). We also discuss carbon ion therapy along with combinatorial immune-based therapies and high-LET RT. For each RT modality, we summarise relevant DNA damage studies. Finally, we provide an update of the role of DNA repair in high-LET RT and we explore the biological responses triggered by differential LET and dose.
Investigation of Dual Airborne Laser Scanners for Detection and State Estimation of Mobile Obstacles in an Aircraft External Hazard Monitor

SMEARCHECK, MARK A. , M.S., June 2008, Electrical Engineering Investigation of Dual Airborne Laser Scanners for Detection and State Estimation of Mobile Obstacles in an Aircraft External Hazard Monitor (124 pp.) Director of Thesis: Maarten Uijt de Haag To ensure aircraft safety during precision approach procedures detailed information pertaining to hazards located on the runway and surroundings area must be known. In order to identify possible safety threats, aircraft could be equipped with sensors capable of detecting all significant hazards in a variety of weather conditions and landing scenarios. An analysis of potential hazards and sensor capabilities serves as the basis of this research. One such hazard monitoring implementation is proposed that makes use of two airborne laser scanners, GPS, and an inertial measurement unit. The goal of this implementation is to detect a ground vehicle driving on the runway during aircraft precision approach. Capabilities of the system include accurate geo-referencing of laser footprints, hazard detection and classification, and hazard state estimation. These goals are accomplished with digital surface modeling using sensor and navigation information, a measurement classification method using a windowed weighted least squares algorithm, and unsupervised clustering. Experimental flight-testing has been performed to collect aircraft navigation data, laser measurements of a vehicle in motion on the runway, and vehicle position truth data. Results indicate geo-referencing accuracy of approximately 2 m in most cases, along with successful hazard classification, and hazard velocity estimates accurate to within 2.8 m/s. Approved: _____________________________________________________________ Maarten Uijt de Haag Associate Professor of Electrical Engineering and Computer Science
On possible trypsin‐induced biases in peptides analysis with aerolysin nanopore

Nanopore‐based single‐molecule analysis technique is a promising approach in the field of proteomics. In this Technical Brief, the interaction between the biological nanopore of Aerolysin (AeL) and peptides is investigated, focusing on potential biases depending on the AeL activation protocol. Our results reveal that residual trypsin, which may be unintentionally introduced in analyte solution when using a classical AeL activation protocol, can induce a significant formation of shorter peptides by enzymatic degradation of longer ones, which may lead to unwanted effects and/or misinterpretations. AeL free‐trypsin activation protocol eliminates this bias and appears more appropriate for peptide/proteins analysis, specifically in the perspective of nanopore‐based molecular fingerprinting or of low‐abundance species characterization.
Viscosity of bridgmanite determined by in situ stress and strain measurements in uniaxial deformation experiments

To understand mantle dynamics, it is important to determine the rheological properties of bridgmanite, the dominant mineral in Earth’s mantle. Nevertheless, experimental data on the viscosity of bridgmanite are quite limited due to experimental difficulties. Here, we report viscosity and deformation mechanism maps of bridgmanite at the uppermost lower mantle conditions obtained through in situ stress-strain measurements of bridgmanite using deformation apparatuses with the Kawai-type cell. Bridgmanite would be the hardest among mantle constituent minerals even under nominally dry conditions in the dislocation creep region, consistent with the observation that the lower mantle is the hardest layer. Deformation mechanism maps of bridgmanite indicate that grain size of bridgmanite and stress conditions at top of the lower mantle would be several millimeters and ~105 Pa to realize viscosity of 1021–22 Pa·s, respectively. This grain size of bridgmanite suggests that the main part of the lower mantle is isolated from the convecting mantle as primordial reservoirs.
Discrimination Between Healthy Eyes and Those With Mild Glaucoma Damage Using Hemoglobin Measurements of the Optic Nerve Head

Précis: The Laguna ONhE, a software that measures the hemoglobin (Hb) concentration of the optic nerve head (ONH) from fundus photographs, demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma. Purpose: The aim was to evaluate Hb concentration of the optic nerve to distinguish between healthy eyes and eyes with mild glaucoma. Methods: Eyes from patients with mild primary open angle glaucoma (MD > −6 dB) (n=58) and from healthy subjects (n=64) were selected. Retinal nerve fiber layer thickness measurements of all eyes were acquired with optical coherence tomography. Optic disc photographs were also obtained, and the images were analyzed using the Laguna ONhE software, which measures the amount of Hb in 24 sectors of the ONH. The software also calculates the Glaucoma Discriminant Function (GDF), an index that expresses the chance of the ONH being compatible with glaucoma. Areas under the receiver operating characteristic curve and sensitivities at fixed specificities of 90% and 95% of each Laguna ONhE parameter were calculated. Results: The mean retinal nerve fiber layer thickness and vertical cup/disc ratio of the control and glaucoma groups were 90.0±10.6 μm versus 66.28±9.85 μm (P<0.001) and 0.5±0.09 versus 0.65±0.09 (P<0.001), respectively. Total Hb (67.9±4.45 vs. 62.89±4.89, P<0.001) and GDF (11.57±15.34 vs. −27.67±20.94, P<0.001) were significantly higher in the control group. The Hb concentration was also significantly higher in 21 of the 24 sectors in the control group compared with the glaucoma group (P<0.05). The GDF had the largest areas under the receiver operating characteristic curve (0.93), with 79.3% sensitivity at a fixed specificity of 95%. Conclusion: Measurements of optic nerve Hb concentration using a colorimetry photographic device demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma. Further studies are need to understand vascular factors implicated in the development of glaucoma.
Impact of diseases and pests on premature fruit drop in sweet orange orchards in São Paulo state citrus belt, Brazil.

BACKGROUND Despite technical improvements in the citrus chain and leadership in orange production achieved in the past decades, premature fruit drop remains a major component of crop loss in São Paulo state citrus belt, the largest sweet orange production area in the world. The present study aimed to determine, during five consecutive seasons, the impact of the diseases and pests on premature fruit drop in the orange belt. RESULTS Fruit drop due to the main diseases and pests averaged ~11.0%, which corresponded to ~63% of the annual fruit drop. The average fruit drop rate due to fruit borer and fruit flies combined was 4.0%, huanglongbing 3.3%, black spot 2.6%, leprosis 1.0% and citrus canker 0.3%. The average amount of fruit drop (million 40.8 kg boxes) and value of crop losses (million US$ dollars), in five seasons, were 12.7 and 66.2 for fruit borer/fruit flies, 11.0 and 57.9 for HLB, 8.1 and 42.2 for black spot, 3.1 and 15.6 for leprosis, and 0.9 and 4.9 for citrus canker, respectively. CONCLUSION Fruit borer and fruit flies (combined), HLB, black spot, leprosis and citrus canker are, in this order, the main diseases and pests in the orange belt of São Paulo state. All these causes significantly increased the overall fruit drop rate in the evaluated seasons. The results will contribute to the development of the Brazilian citrus industry, while showing to other citrus-growing regions the potential that diseases and pests have to jeopardize production. This article is protected by copyright. All rights reserved.
The sound of silence: Predictive error responses to unexpected sound omission in adults

The human auditory system excels at detecting patterns needed for processing speech and music. According to predictive coding, the brain predicts incoming sounds, compares predictions to sensory input and generates a prediction error whenever a mismatch between the prediction and sensory input occurs. Predictive coding can be indexed in electroencephalography (EEG) with the mismatch negativity (MMN) and P3a, two components of event‐related potentials (ERP) that are elicited by infrequent deviant sounds (e.g., differing in pitch, duration and loudness) in a stream of frequent sounds. If these components reflect prediction error, they should also be elicited by omitting an expected sound, but few studies have examined this. We compared ERPs elicited by infrequent randomly occurring omissions (unexpected silences) in tone sequences presented at two tones per second to ERPs elicited by frequent, regularly occurring omissions (expected silences) within a sequence of tones presented at one tone per second. We found that unexpected silences elicited significant MMN and P3a, although the magnitude of these components was quite small and variable. These results provide evidence for hierarchical predictive coding, indicating that the brain predicts silences and sounds.
Life stage and proximity to roads shape the skin microbiota of eastern newts (Notophthalmus viridescens)

Summary Host‐associated microbiomes play an essential role in the health of organisms, including immune system activation, metabolism and energy uptake. It is well established that microbial communities differ depending on the life stage and natural history of the organism. However, the effects of life stage and natural history on microbial communities may also be influenced by human activities. We investigated the effects of amphibian life stage (terrestrial eft vs. aquatic adult) and proximity to roadways on newt skin bacterial communities. We found that the eft and adult life stages differed in bacterial community composition; however, the effects of roads on community composition were more evident in the terrestrial eft stage compared to the aquatic adult stage. Terrestrial efts sampled close to roads possessed richer communities than those living further away from the influence of roads. When accounting for amplicon sequence variants with predicted antifungal capabilities, in the adult life stage, we observed a decrease in anti‐fungal bacteria with distance to roads. In contrast, in the eft stage, we found an increase in anti‐fungal bacteria with distance to roads. Our results highlight the need to consider the effects of human activities when evaluating how host‐associated microbiomes differ across life stages of wildlife.
Technology in psychology: a bibliometric analysis of technology-based interventions in clinical and health psychology

ABSTRACT This study aimed to identify, synthesize, and evaluate the current state of research on the use of technology-based interventions in clinical psychology through 2017 as a recent innovative area of study. It was intended to provide a critical overview of trends in different tools and populations and identify future areas of interest. This paper focuses on studies published in psychological interventions in childhood, adolescents, adults, and geriatric populations using new technologies, including web-based intervention, virtual reality, augmented reality, mobile applications, and robotics, with particular attention to methodology. To achieve this aim, a systematic search was made in the ISI Web of Science for intervention, psychology, and the technological tools previously mentioned. The results of the study show that the use of information and communication technologies in psychology has been an innovative and growing field of study for the last 10 years. In total, 743 were included in this study. A growing trend has been observed in publications related to psychology and the use of technologies since 2007. Resea0rch topics were focused mainly on interventions on specific problems or disorders such as depression. The largest number of publications were found for the web-based intervention, in randomized clinical trials and applied to adults
Multiscale Hierarchical Architecture‐Based Bioactive Scaffolds for Versatile Tissue Engineering

Artificial construction from tendon to bone remains a formidable challenge in tissue engineering owing to their structural complexity. In this work, bioinspired calcium silicate nanowires and alginate composite hydrogels are utilized as building blocks to construct multiscale hierarchical bioactive scaffolds for versatile tissue engineering from tendon to bone. By integrating 3D printing technology and mechanical stretch post‐treatment in a confined condition, the obtained composite hydrogels possess bioinspired reinforcement architectures from nano‐ to submicron‐ to microscale with significantly enhanced mechanical properties. The biochemical and topographical cues of the composite hydrogel scaffolds provide much more efficient microenvironment to the rabbit bone mesenchymal stem cells and rabbit tendon stem cells, leading to ordered alignment and improved differentiation. The composite hydrogels markedly promote in vivo tissue regeneration from bone to tendon, especially fibrocartilage transitional tissue. Therefore, such calcium silicate nanowires/alginate composite hydrogels with multiscale hierarchical structures have potential application for tissue regeneration from tendon to bone. This work provides an innovative strategy to construct multiscale hierarchical architecture‐based scaffolds for tendon/bone engineering.
Comparison of Anterior Segment Abnormalities in Individuals With FOXC1 and PITX2 Variants

Supplemental Digital Content is Available in the Text. Purpose: Axenfeld–Rieger syndrome encompasses a group of developmental disorders affecting the anterior chamber structures of the eye, with associated systemic features in some cases. This study aims to compare the difference in anterior segment phenotypes such as those involving the cornea, iris, lens, and anterior chamber angle between cases with disease-causing sequence variations in FOXC1 and PITX2. Methods: This cross-sectional study involved 61 individuals, from 32 families with pathogenic FOXC1 or PITX2 variants, who were registered with the Australian and New Zealand Registry of Advanced Glaucoma. Results: The median age of the cohort was 39 years at the time of last assessment (range 3–85 years; females, 54%). Thirty-two patients had pathogenic variants in the FOXC1 gene, and 29 patients had pathogenic variants in the PITX2 gene. Corneal abnormalities were more common in individuals with FOXC1 variants (18/36, 50%) than those with PITX2 variants (4/25, 16%; P = 0.007). Iris abnormalities such as hypoplasia (P = 0.008) and pseudopolycoria (P = 0.001) were more common in individuals with PITX2 variants than those with FOXC1 variants. Glaucoma was present in 72% of participants. Corneal decompensation was positively associated with corneal abnormalities (P < 0.001), glaucoma surgery (P = 0.025), and cataract surgery (P = 0.002). Conclusions: Corneal abnormalities were more common in individuals with FOXC1 than in those with PITX2 variants and were often associated with early onset glaucoma. These findings highlight that patients with FOXC1 variations require close follow-up and monitoring throughout infancy and into adulthood.
Size, weight, and expectations

The size-weight illusion is well-known: if two equally heavy objects differ in size, the large one feels lighter than the small one. Most explanations for this illusion assume that because the information about the relevant attribute (weight itself) is unreliable, information about an irrelevant but correlated attribute (size) is used as well. If such reasoning is correct, one would expect that the illusion can be inverted: if size information is unreliable, weight information will be used to judge size. We explored whether such a weight-size illusion exists by asking participants to lift Styrofoam balls that were coated with glow in the dark paint. The balls (2 sizes, 3 weights) were lifted using a pulley system in complete darkness at 2 distances. Participants reported the size using free magnitude estimation. The visual size information was indeed unreliable: balls that were presented at a 20% larger distance were judged 15% smaller. Nevertheless, the judgments of size were not systematically affected by the 20% weight change (differences < 0.5%). We conclude that because the weight-size illusion does not exist, the mechanism behind the size-weight illusion is specific for judging heaviness.
RNA is required for the integrity of multiple nuclear and cytoplasmic membrane‐less RNP granules

Numerous membrane‐less organelles, composed of a combination of RNA and proteins, are observed in the nucleus and cytoplasm of eukaryotic cells. These RNP granules include stress granules (SGs), processing bodies (PBs), Cajal bodies, and nuclear speckles. An unresolved question is how frequently RNA molecules are required for the integrity of RNP granules in either the nucleus or cytosol. To address this issue, we degraded intracellular RNA in either the cytosol or the nucleus by the activation of RNase L and examined the impact of RNA loss on several RNP granules. We find the majority of RNP granules, including SGs, Cajal bodies, nuclear speckles, and the nucleolus, are altered by the degradation of their RNA components. In contrast, PBs and super‐enhancer complexes were largely not affected by RNA degradation in their respective compartments. RNA degradation overall led to the apparent dissolution of some membrane‐less organelles, whereas others reorganized into structures with altered morphology. These findings highlight a critical and widespread role of RNA in the organization of several RNP granules.
Insulin enhances neurite extension and myelination of diabetic neuropathy neurons

Background The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.