Miracle-dz/UniFAR-FTUs huggingface dataset:
Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes

Simple Summary Rooster sperm cryopreservation is the preferred technique for genetic preservation, particularly in indigenous breeds whose numbers are decreasing. However, cryopreserved semen is highly damaged, mainly from cold shock and oxidative stress during freezing, leading to membrane and sperm damage. It is necessary to protect sperm membranes against peroxidative damage to improve semen quality and fertility. This study aimed to determine the optimal ginseng concentration used as a freezing extender in cryopreserved Thai native rooster semen. Ginsenosides are active components extracted from ginseng that exert antioxidant effects that inhibit oxidative stress. Our results showed that ginseng extract dosage in the freezing extender influenced frozen-thawed semen quality, improved motility and membrane integrity, reduced oxidative stress caused by the freezing process, enhanced enzyme activity in seminal plasma, and increased fertility in rooster semen cryopreservation. Abstract To the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage method and then pooled and divided into five groups according to the concentrations of ginseng supplementation (0, 0.25, 0.50, 0.75, and 1 mg/mL) in a freezing extender. The semen suspensions were loaded into a medium straw and cryopreserved using the liquid nitrogen vapor method. The post-thaw semen was evaluated for sperm quality (sperm motility and membrane integrity), seminal plasma characteristics (lipid peroxidation, superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), and fertility. The results showed that ginseng extract supplementation at 0.25 mg/mL yielded the highest total motility, progressive motility, and membrane integrity (59.47%, 30.82%, and 48.30%, respectively; p < 0.05) in cryopreserved rooster semen. Higher malondialdehyde concentrations were observed in the control group than in the other groups (p < 0.05). SOD, CAT, and GPx increased compared with those in the control group (p < 0.05). The results showed that the fertility rate with 0.25 mg/mL of ginseng was higher than that of the control group (62.80% vs. 46.28%: p < 0.05). In conclusion, supplementation with 0.25 mg/mL of ginseng is recommended as an alternative component to the freezing extender to improve rooster semen cryopreservation.
Myhre syndrome

Myhre syndrome (MS) is a developmental disorder characterized by typical facial dysmorphism, thickened skin, joint limitation and muscular pseudohypertrophy. Other features include brachydactyly, short stature, intellectual deficiency with behavioral problems and deafness. We identified SMAD4 as the gene responsible for MS. The identification of SMAD4 mutations in Laryngotracheal stenosis, Arthropathy, Prognathism and Short stature (LAPS) cases supports that LAPS and MS are a unique entity. The long‐term follow up of patients shows that these conditions are progressive with life threatening complications. All mutations are de novo and changing in the majority of cases Ile500, located in the MH2 domain involved in transcriptional activation. We further showed an impairment of the transcriptional regulation via TGFβ target genes in patient fibroblasts. Finally, the absence of SMAD4 mutations in three MS cases may support genetic heterogeneity.
State space modelling approach for rudder roll stabilization

The control objective of the Rudder Roll Stabilization (RRS) system is to deploy the rudder, which is primarily a path controlling device, to reduce the roll motion without interference in heading of ship. To achieve the control of both roll and yaw motions, the only control input is the rudder angle and hence the RRS system is referred as a Single Input, Two Output (SITO) system. Rudder roll stabilization is insignificant at low forward speed of the ship, but can give significant control at higher speed when fast rudder movement is applied. This paper presents a closed loop state space model for accurate simulations on rudder roll stabilization in irregular seas considering the 3-degree of freedom motions, i.e., sway, roll and yaw. The computational model is developed to analyze the effect of the rudder movement on sway, roll and yaw in forward speed conditions in irregular sea conditions. The Sea State conditions are modelled as wave perturbation models using the method of shaping filter established by filtered white noise. The control system has been designed using optimal linear quadratic regulator (LQR) method. The control loop contains both the signal for the autopilot action to trigger the heading angle correction as well as the signal for rudder based roll motion control. The simulations are carried out with rudder roll control system ON and OFF mode to analyze the effect of the rudder on steering and motion stabilization. In both cases the autopilot is in active mode to correct deviations in the course heading. The simulations are analyzed for three different ship speeds in two different Seas State conditions with a low and fast rudder movement to show the efficacy of the model. The performance is evaluated and presented based on the RMS value. Since the rudder based roll motion stabilization may also result in unnecessary motions of sway and yaw, besides the desirable roll reduction, the result presents the sway-roll-yaw responses as applicable under the particular speed and Sea State conditions.
Laboratory demonstration of the triple-grating vector vortex coronagraph

The future Habitable Worlds Observatory aims to characterize the atmospheres of rocky exoplanets around solar-type stars. The Vector Vortex Coronagraph (VVC) is a main candidate to reach the required contrast of 10−10. However, the VVC requires polarization filtering and every observing band requires a different VVC. The triple-grating vector vortex coronagraph (tgVVC) aims to mitigate these limitations by combining multiple gratings that minimize the polarization leakage over a large spectral bandwidth. In this paper, we present laboratory results of a tgVVC prototype using the In-Air Coronagraphic Testbed (IACT) facility at NASA’s Jet Propulsion Laboratory and the Space Coronagraph Optical Bench (SCoOB) at the University of Arizona Space Astrophysics Lab (UASAL). We study the coronagraphic performance with polarization filtering at 633 nm and reach a similar average contrast of 2 × 10−8 between 3-18 λ/D at the IACT, and 6 × 10−8 between 3-14 λ/D at SCoOB. We explore the limitations of the tgVVC by comparing the testbed results. We report on other manufacturing errors and ways to mitigate their impact.
THE EFFECTIVENESS OF DEBT RULE ON FISCAL SUSTAINABILITY: DO MACROECONOMIC DATA MATTER?

The effectiveness of the debt rule to achieve a certain level of public debt and debt sustainability targets is one of the fiscal sustainability discussed in the literature. The current study attempts to estimate the impact of the debt rule on fiscal sustainability indicators for 77 countries during 1985 - 2018. There are two existing indicators are widely utilized cover primary gap and recursive algorithm. To deal with the potential of a random walk of the variables, the two-step system generalized method of moment (GMM) estimator is employed. Specifically, the current study constructs a new fiscal sustainability indicator by incorporating financial technology (FinTech) using principal component analysis (PCA) for 67 countries in 2014 and 2017. The new indicator is the main contribution on the existing literature of fiscal sustainability. The findings reveal that the debt rule has a positive and significant impact on the primary gap, either in the short- or medium-term, implying that the debt rule is effective in encouraging fiscal sustainability. Conversely, it has a negative and significant impact on the recursive algorithm. Similarly, this rule significantly contributes on the new indicator by incorporating FinTech. Therefore, policymakers are challenged to conduct this rule as a key fiscal rule in fiscal sustainability policy. The policymakers should also take more attention to increase the level of FinTech to guarantee fiscally sustainable level.
Botulinum Toxin—A High-Dosage Effect on Functional Outcome and Spasticity-Related Pain in Subjects with Stroke

Stroke patients can develop spasticity and spasticity-related pain (SRP). These disorders are frequent and can contribute to functional limitations and disabling conditions. Many reports have suggested that higher doses than initially recommended of BTX-A can be used effectively and safely, especially in the case of severe spasticity; however, whether the treatment produces any benefit on the functional outcome and SRP is unclear. Studies published between January 1989 and December 2022 were retrieved from MEDLINE/PubMed, Embase, and Cochrane Central Register. Only obabotulinumtoxinA (obaBTX-A), onabotulinumtoxinA, (onaBTX-A), and incobotulinumtoxinA (incoBTX-A) were considered. The term “high dosage” indicates ≥600 U. Nine studies met the inclusion criteria. Globally, 460 subjects were treated with BTX-A high dose, and 301 suffered from stroke. Studies had variable method designs, sample sizes, and aims. Only five (55.5%) reported data about the functional outcome after BTX-A injection. Functional measures were also variable, and the improvement was observed predominantly in the disability assessment scale (DAS). SRP pain was quantified by visual analog scale (VAS) and only three studies reported the BTX-A effect. There is no scientific evidence that this therapeutic strategy unequivocally improves the functionality of the limbs. Although no clear-cut evidence emerges, certain patients with spasticity might obtain goal-oriented improvement from high-dose BTX-A. Likewise, data are insufficient to recommend high BTX dosage in SRP.
Plasma phosphorylated tau181 as a biomarker of mild traumatic brain injury: findings from THINC and NCAA-DoD CARE Consortium prospective cohorts

Objective The aim of this study was to investigate phosphorylated tau (p-tau181) protein in plasma in a cohort of mild traumatic brain injury (mTBI) patients and a cohort of concussed athletes. Methods This pilot study comprised two independent cohorts. The first cohort—part of a Traumatic Head Injury Neuroimaging Classification (THINC) study—with a mean age of 46 years was composed of uninjured controls (UIC, n = 30) and mTBI patients (n = 288) recruited from the emergency department with clinical computed tomography (CT) and research magnetic resonance imaging (MRI) findings. The second cohort—with a mean age of 19 years—comprised 133 collegiate athletes with (n = 112) and without (n = 21) concussions. The participants enrolled in the second cohort were a part of a multicenter, prospective, case-control study conducted by the NCAA-DoD Concussion Assessment, Research and Education (CARE) Consortium at six CARE Advanced Research Core (ARC) sites between 2015 and 2019. Blood was collected within 48 h of injury for both cohorts. Plasma concentration (pg/ml) of p-tau181 was measured using the Single Molecule Array ultrasensitive assay. Results Concentrations of plasma p-tau181 in both cohorts were significantly elevated compared to controls within 48 h of injury, with the highest concentrations of p-tau181 within 18 h of injury, with an area under the curve (AUC) of 0.690–0.748, respectively, in distinguishing mTBI patients and concussed athletes from controls. Among the mTBI patients, the levels of plasma p-tau181 were significantly higher in patients with positive neuroimaging (either CT+/MRI+, n = 74 or CT−/MRI+, n = 89) compared to mTBI patients with negative neuroimaging (CT−/MRI−, n = 111) findings and UIC (P-values < 0.05). Conclusion These findings indicate that plasma p-tau181 concentrations likely relate to brain injury, with the highest levels in patients with neuroimaging evidence of injury. Future research is needed to replicate and validate this protein assay's performance as a possible early diagnostic biomarker for mTBI/concussions.
ErbB2 (HER2)-CAR-NK-92 cells for enhanced immunotherapy of metastatic fusion-driven alveolar rhabdomyosarcoma

Introduction Metastatic rhabdomyosarcoma (RMS) is a challenging tumor entity that evades conventional treatments and endogenous antitumor immune responses, highlighting the need for novel therapeutic strategies. Applying chimeric antigen receptor (CAR) technology to natural killer (NK) cells may offer safe, effective, and affordable therapies that enhance cancer immune surveillance. Methods Here, we assess the efficacy of clinically usable CAR-engineered NK cell line NK-92/5.28. z against ErbB2-positive RMS in vitro and in a metastatic xenograft mouse model. Results Our results show that NK-92/5.28. z cells effectively kill RMS cells in vitro and significantly prolong survival and inhibit tumor progression in mice. The persistence of NK-92/5.28. z cells at tumor sites demonstrates efficient antitumor response, which could help overcome current obstacles in the treatment of solid tumors. Discussion These findings encourage further development of NK-92/5.28. z cells as off-the-shelf immunotherapy for the treatment of metastatic RMS.
The Usage Attitude of Innovative Online Food Ordering Applications of Employees in Laem Chabang Industrials Estate, Chonburi

The pandemic of Coronavirus resulted in the change of ordering food method. Online ordering food was the new one that was famous in this period. The purposes of this research are 1) to study the usage attitude of online food ordering technology of employees who work at Laem Chabang industrial estate, 2) to study the factors that affect the usage attitude of online food ordering technology of employees who work at Laem Chabang industrial estate. The data were collected through a self-administered survey questionnaire. The target population is employees who work at Laem Chabang industrial estate. Purposive sampling is used for the collection of data from 400 employees. The data were analyzed through multiple regression. The results revealed that 1) perceived ease of use of online food ordering applications has a positive influence on the Perceived usefulness of online food ordering applications with a statistically significant level of 0.05, 2) perceived ease of use of online ordering food applications has a negative influence on Behavioral intention to use of online food ordering application with a statistically significant level of 0.05, 3) perceived usefulness of online food ordering applications has a positive influence on Behavioral intention to use of online food ordering application with a statistically significant level of 0.05.
Anti-Glycolipid Antibody Examination in Five EAE Models and Theiler’s Virus Model of Multiple Sclerosis: Detection of Anti-GM1, GM3, GM4, and Sulfatide Antibodies in Relapsing-Remitting EAE

Anti-glycolipid antibodies have been reported to play pathogenic roles in peripheral inflammatory neuropathies, such as Guillain–Barré syndrome. On the other hand, the role in multiple sclerosis (MS), inflammatory demyelinating disease in the central nervous system (CNS), is largely unknown, although the presence of anti-glycolipid antibodies was reported to differ among MS patients with relapsing-remitting (RR), primary progressive (PP), and secondary progressive (SP) disease courses. We investigated whether the induction of anti-glycolipid antibodies could differ among experimental MS models with distinct clinical courses, depending on induction methods. Using three mouse strains, SJL/J, C57BL/6, and A.SW mice, we induced five distinct experimental autoimmune encephalomyelitis (EAE) models with myelin oligodendrocyte glycoprotein (MOG)35–55, MOG92–106, or myelin proteolipid protein (PLP)139–151, with or without an additional adjuvant curdlan injection. We also induced a viral model of MS, using Theiler’s murine encephalomyelitis virus (TMEV). Each MS model had an RR, SP, PP, hyperacute, or chronic clinical course. Using the sera from the MS models, we quantified antibodies against 11 glycolipids: GM1, GM2, GM3, GM4, GD3, galactocerebroside, GD1a, GD1b, GT1b, GQ1b, and sulfatide. Among the MS models, we detected significant increases in four anti-glycolipid antibodies, GM1, GM3, GM4, and sulfatide, in PLP139–151-induced EAE with an RR disease course. We also tested cellular immune responses to the glycolipids and found CD1d-independent lymphoproliferative responses only to sulfatide with decreased interleukin (IL)-10 production. Although these results implied that anti-glycolipid antibodies might play a role in remissions or relapses in RR-EAE, their functional roles need to be determined by mechanistic experiments, such as injections of monoclonal anti-glycolipid antibodies.
Validation of stable p-type phosphorus (P) doped ZnO thin films formation by investigating the photoresponse properties of P:ZnO/Ga2O3 heterojunctions

The formation of reproducible p-type conductivity in ZnO thin films is highly challenging now a days for the fabrication of several homo/heterojunction based fully transparent opto-electronic devices. In this study, p-type P: ZnO thin films are deposited by cost-effective SOD process and then intrinsically n-type Ga2O3 films are deposited on it to validate the p-type conductivity of ZnO by making vertical heterojunction with n-Ga2O3. The ZnO thin films are deposited by RF sputtering and subsequent P-doping is done by using the SOD technique on it. This involves proximity diffusing dopants into a spin-coated film by stacking the dopant source during thermal annealing at 800◦C for four hours in the furnace. Ga2O3 films are deposited on the P: ZnO films by using RF sputtering technique, for making the heterojunction. The electrical measurements are performed by using current-voltage (I-V) measurements under illuminated and dark conditions. The photo-switching and responsivity are also measured on the fabricated device. It is observed that the P: ZnO/Ga2O3 heterojunction exhibits the photoresponse in the dual wavelength region. The corresponding two peaks of responsivity are found around 200 nm and 390 nm with the values of 68.03 A/W and 7.93 A/W (at 5 V), respectively. Such two peaks originated due to the ultra-wide bandgaps of Ga2O3 (4.7eV) and P: ZnO (3.1 eV). Also, such heterojunction shows a rapid switching speed under white light at 5 V (rise time: 230 ms, fall time: 163 ms) and −5 V (rise time: 83 ms, Fall time: 169 ms), which is comparable with the other reported results. Therefore, the current study demonstrates the development of highly stable and reproducible p-type P: ZnO thin films by employing SOD technique and the validation of p-type formation by fabricating P: ZnO/Ga2O3 heterojunctions for dual-wavelength selector UV detector application and such detectors can be a potential candidate for various optoelectronic devices.
Streamlining Precision: The Welding and Fabrication Laboratory Information System

The Welding and Fabrication Laboratory Information System represents a pivotal advancement in enhancing precision and efficiency within the welding and fabrication industries. This study investigates the system's impact by employing a mixed-methods approach that combines qualitative interviews and quantitative surveys to capture insights from technicians, administrators, and clients. The system's streamlined processes, real-time progress tracking, and intuitive user interface have led to significant improvements. Barcode scanning has reduced material identification time by 60%, and progress tracking received a 90% approval rate for enhanced project visibility. Comparing the new system with traditional methods, a 35% reduction in rework costs and a 75% decrease in material stockouts showcase its value. Furthermore, technicians reported an 85% increase in workflow efficiency. The study underscores the transformative potential of the Welding and Fabrication Laboratory Information System in revolutionizing information management, process optimization, and industry competitiveness
Morphometric Assessment of the Internal Auditory Canal for Sex Determination in Subadults Using Cone Beam Computed Tomography (CBCT)

iii Acknowledgements iv Dedication vi List of Tables x List of Figures xv Chapter 1 : Introduction 1 Research Questions and Hypotheses 4 Chapter 2 : Literature Review 5 Sexual Dimorphism in the Human Skeleton 5 Methods of Sex Estimation 6 The Temporal Bone and the Pars Petrosa Temporalis 9 The Lateral Angle Method 11 Tomographic Imaging Techniques 12 The Diameter Method 16 Chapter 3 : Methodology 18 Sampling Procedure 18 Adjustment for Head Position, Brightness, and Contrast 19
FACTORS OF SUSTAINABLE COMPETITIVENESS AT COMPANY LEVEL: A COMPARISON OF FOUR GLOBAL ECONOMIC SECTORS

Built on the premise that a company’s competitiveness is given by its robust financial performance and the strength of its position on the market, but only under conditions of sustainability, this study aims to investigate the key factors of company competitiveness while considering sustainability issues. The two research objectives were: (1) to identify and analyze the key factors of sustainable competitiveness at company level (2) to find sector-related discrepancies regarding the identified factors. To achieve them, a horizontal analysis covering ten financial years (2012–2021) was performed on a sample of 1,449 companies from four global economic sectors (Consumer Cyclicals, Energy, Health Care, and Technology) – using Exploratory factor analysis (EFA) and ANOVA. Nine factors (both financial and non-financial) were found: liquidity, profitability, revenue efficiency, inventory management efficiency, ESG performance, receivables management efficiency, R&D efficiency, book value, and market value. Of them, ESG performance has emerged as an independent factor based on non-financial variables, its introduction into the research model being one of the study’s novelties. Significant mean differences were found between the nine factors, depending on the sector, which allowed the ranking of sectors in terms of sustainable competitiveness, in descending order: Health Care, Technology, Consumer Cyclicals, and Energy.
Keunggulan Bersaing dan Loyalitas Pelanggan pada Distribusi Impor Beras Khusus di Indonesia

Importing the specific rice could only be done by BUMN (Badan Usaha Milik Negara) which in this article represented by PT DFG. The company is still lack of supervision of the customer relationship management implementation due to the absence of the special department and also lack of supervision of distribution. The study used Path Analysis with 35 distributor companies as sample. The purpose of the research is to analyze, directly and indirectly, the variables of customer relationship management and supply chain management on customers’ loyalty of imported specific rice intervened by competitive advantage variables. The results of the study show that the competitive advantage cannot act as an intervening variable and has an indirect effect on customer relationship management of the rice commodity customers’ loyalty. On the other hand, the competitive advantage can act as an intervening variable and has an indirect effect on supply chain management of the specific rice customers’ loyalty. Thus, the changes of the supply chain management implementation in PT DFG can affect customers’ loyalty indirectly through competitive advantage.