Miracle-dz/UniFAR-FTUs huggingface dataset:
The effect of mutation status, pathological features and tumor location on prognosis ın patients with colorectal cancer.

OBJECTIVE Colorectal cancer is the most common malignancy of the gastrointestinal tract. It is the third most common tumor in both genders and the second reason of cancer-related deaths. In recent years, tumor location has gained importance as a prognostic indicator. In this study, we aimed to analyze if there was a prognostic effect of tumor location, the pathological features, and the mutation status of patients on survival. METHODS Two-hundred and ten colorectal cancer patients aged 18 years and older were included into the study. One-hundred and forty-two patients had left-sided tumor and 68 patients had right-sided tumor. Patients who had other malignancies rather than squamous cell skin cancer and in situ cervical cancer were excluded. All statistical tests were carried out using two-sided process, and a p≤0.05 was considered statistically significant. RESULTS There were 140 men and 70 women in the study. The median age of the patients was 62 years old. There was no statistically significant difference according to tumor location and survival of patients. The overall survival of patients with right-sided tumors was 60.5 months and 47.2 months for left-sided tumors. Disease-free survival of patients was 63.7 months for right-sided tumors and 46 months for left-sided ones. Perineural invasion, grade and stage were crucial prognostic parameters. Disease-free survival was longer for female colorectal cancer patients. CONCLUSION According to our study, survival of patients was similar regardless of tumor location. This can be explained by the different sequencing of treatment strategies and divergent population genetics.
Effect of catalysts and precursors in the synthesis of carbon nanotubes by catalytic chemical vapour deposition

Since the pioneering report of discovery of carbon nanotubes (CNTs) in 1991 by Iijima, scientists and researchers worldwide have carried out in depth investigations in this new family of carbon because of its myriad properties and potential applications. The synthesis of novel nanoscale material is the main target in current material science. This study investigates the effect of different types of cabon source and and catalyst on the type of CNTs formed via catalytic chemical vapour deposition (CCVD) method. Three types of carbon source i.e. acetylene, methane and ethanol were used for the synthesis of CNTs. The catalysts used in the synthesis of CNTs are monometallic, bimetallic and trimetallic derived from Fe, Co and Ni salts using wet impregnation method. The catalysts were characterized by scanning electron microscope (SEM) and energy-dispersive X-ray analysis (EDX). The analysis confirmed the presence of Fe, Co and Ni. The as-synthesized CNTs were characterized using SEM/field emission-scanning electron microscope (FE-SEM), EDX, Raman spectroscopy and transmission electron microscopy (TEM). This analysis also confirmed that all the prepared catalysts were active for the production of CNTs. SEM/FE-SEM analysis revealed different morphologies of CNTs were formed when different catalysts and carbon source were used. Raman spectra revealed that acetylene and methane precursor produced multi-walled carbon nanotubes (MWNTs) as indicated by the presence of G-band and D-band peaks. However, their structures were different depending on the catalysts used. Meanwhile, the presence of RBM peaks along with the G-band and D-band revealed that singlewalled carbon nanotubes (SWNTs) are produced using ethanol as the carbon source. TEM micrographs obtained confirmed that acetylene and methane produced MWNTs and ethanol produced SWNTs with diameter in the range of 14.74-34.59 nm, 10.19-37.61 nm and 0.96-2.52 nm, respectively. However, Fe/Al2O3 catalyst selectively produced double-walled carbon nanotubes (DWNTs) when ethanol was used as the carbon source. Generally, this research has been successful in producing various types of CNTs depending on the catalyst used and the carbon source selected
Patterns of post‐drought recovery are strongly influenced by drought duration, frequency, post‐drought wetness, and bioclimatic setting

Understanding vegetation recovery after drought is critical for projecting vegetation dynamics in future climates. From 1997 to 2009, Australia experienced a long‐lasting drought known as the Millennium Drought (MD), which led to widespread reductions in vegetation productivity. However, vegetation recovery post‐drought and its determinants remain unclear. This study leverages remote sensing products from different sources—fraction of absorbed photosynthetically active radiation (FPAR), based on optical data, and canopy density, derived from microwave data—and random forest algorithms to assess drought recovery over Australian natural vegetation during a 20‐year period centered on the MD. Post‐drought recovery was prevalent across the continent, with 6 out of 10 drought events seeing full recovery within about 6 months. Canopy density was slower to recover than leaf area seen in FPAR. The probability of full recovery was most strongly controlled by drought return interval, post‐drought hydrological condition, and drought length. Full recovery was seldom observed when drought events occurred at intervals of 3 months or less, and moderately dry (standardized water balance anomaly [SWBA] within [−1, −0.76]) post‐drought conditions resulted in less complete recovery than wet (SWBA > 0.3) post‐drought conditions. Press droughts, which are long term but not extreme, delayed recovery more than pulse droughts (short term but extreme) and led to a higher frequency of persistent decline. Following press droughts, the frequency of persistent decline differed little among biome types but peaked in semi‐arid regions across aridity levels. Forests and savanna required the longest recovery times for press drought, while grasslands were the slowest to recover for pulse drought. This study provides quantitative thresholds that could be used to improve the modeling of ecosystem dynamics post‐drought.
Complicated Carriage with Methicillin-Resistant Staphylococcus aureus: Evaluation of the Effectiveness of Decolonization Regimens Advised in the Dutch National Guideline

Methicillin-resistant Staphylococcus aureus (MRSA) colonization leads to increased infection rates and mortality. Decolonization treatment has been proven to prevent infection and reduce transmission. ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) colonization leads to increased infection rates and mortality. Decolonization treatment has been proven to prevent infection and reduce transmission. As the optimal antimicrobial strategy is yet to be established, different regimens are currently prescribed to patients. This study aimed to evaluate the efficacy of the decolonization treatments recommended by the Dutch guideline. A retrospective multicenter cohort study was conducted in five Dutch hospitals. All patients who visited the outpatient clinic because of complicated MRSA carriage between 2014 and 2018 were included. We obtained data on patient characteristics, clinical and microbiological variables relevant for MRSA decolonization, environmental factors, decolonization regimen, and treatment outcome. The primary outcome was defined as three negative MRSA cultures after treatment completion. Outcomes were stratified for the first-line treatment strategies. A total of 131/224 patients were treated with systemic antibiotic agents. Treatment was successful in 111/131 (85%) patients. The success rate was highest in patients treated with doxycycline-rifampin (32/37; 86%), but the difference from any of the other regimens did not reach statistical significance. There was no difference in the success rate of a 7-day treatment compared to that with 10 to 14 days of treatment (odds ratio [OR], 0.99; 95% confidence interval [CI], 0.39 to 2.53; P = 1.00). Side effects were reported in 27/131 (21%) patients and consisted mainly of mild gastrointestinal complaints. In a multivariable analysis, an immunocompromised status was an independent risk factor for failure at the first treatment attempt (OR, 4.65; 95% CI, 1.25 to 17.25; P = 0.02). The antimicrobial combinations recommended to treat complicated MRSA carriage yielded high success rates. Prolonged treatment did not affect treatment outcome. A randomized trial is needed to resolve whether the most successful regimen in this study (doxycycline plus rifampin) is superior to other combinations.
Poly(L-lactide) macrodiols (HOPLLAOH): Influence of linear alkyl diols as initiators: Synthesis and characterization

ABSTRACT A series of α,ω-hydroxy telechelic poly(L-lactide)s (HOPLLAOHs) were synthesized by ring-opening polymerization (ROP) of L-lactide (L-LA) using tin octoate [Sn(Oct)2] as catalyst and a family of linear alkyl diols as initiators [HO–[CH2]m–OH, where m = 2, 4, 6, 8, 10, and 12]. A systematic analysis of these HOPLLAOHs species in terms of their thermal properties was realized by DSC. In this sense, the linear alkyl group had an important influence on the glass transition temperature (Tg); a relatively high content of alkyl group on the HOPLLAOH increased the flexibility of the polyester, evidenced by a value of Tg inversely proportional to the weight percent of the alkyl group. Besides, the alkyl groups had an effect on the crystallization temperature (Tc), melting temperature (Tm), and crystallinity (xi). Additionally, HOPLLAOHs were characterized by 1H and 13C NMR, FT-IR, MALDI-TOF, and GPC.
Recent lessons learned for ex-vivo platelet production

Purpose of review Platelet transfusion can be life-saving but carries a risk of infection or alloimmunization and is limited by insufficient donor sources and restricted unit shelf life. Generating sufficient platelets in vitro to replace a unit of collected blood remains a challenge. Here, we examine the latest advances in the regulation of megakaryocyte maturation and expansion along with platelet formation and survival. We also discuss alternative therapies investigated to induce platelet production. Recent findings Recent studies examined candidate niche cells in the bone marrow microenvironment for promoting platelet formation and developed an explant-based bioreactor to enhance platelet production ex vivo. Chemical inhibitors were examined for their ability to promote megakaryocyte maturation and expansion. Microparticles from megakaryocytes or platelets were found to improve megakaryocyte maturation and platelet formation. Membrane budding was identified as a novel mode of platelet formation. Lastly, a chemical inhibitor to improve cold-stored platelets was identified. Summary Recent advances in the regulation of megakaryocyte expansion and platelet production provide exciting promise for the development of improved approaches to generate platelets in vitro. These findings bring the field one step closer to achieving the ultimate goal of creating a unit of platelets without the need for donation.
Diabetes Exacerbates the Intraocular Pressure-Independent Retinal Ganglion Cells Degeneration in the DBA/2J Model of Glaucoma

Purpose Glaucoma is a multifactorial disease, causing retinal ganglion cells (RGCs) and optic nerve degeneration. The role of diabetes as a risk factor for glaucoma has been postulated but still not unequivocally demonstrated. The purpose of this study is to clarify the effect of diabetes in the early progression of glaucomatous RGC dysfunction preceding intraocular pressure (IOP) elevation, using the DBA/2J mouse (D2) model of glaucoma. Methods D2 mice were injected with streptozotocin (STZ) obtaining a combined model of diabetes and glaucoma (D2 + STZ). D2 and D2 + STZ mice were monitored for weight, glycemia, and IOP from 3.5 to 6 months of age. In addition, the activity of RGC and outer retina were assessed using pattern electroretinogram (PERG) and flash electroretinogram (FERG), respectively. At the end point, RGC density and astrogliosis were evaluated in flat mounted retinas. In addition, Müller cell reactivity was evaluated in retinal cross-sections. Finally, the expression of inflammation and oxidative stress markers were analyzed. Results IOP was not influenced by time or diabetes. In contrast, RGC activity resulted progressively decreased in the D2 group independently from IOP elevation and outer retinal dysfunction. Diabetes exacerbated RGC dysfunction, which resulted independent from variation in IOP and outer retinal activity. Diabetic retinas displayed decreased RGC density and increased glial reactivity given by an increment in oxidative stress and inflammation. Conclusions Diabetes can act as an IOP-independent risk factor for the early progression of glaucoma promoting oxidative stress and inflammation-mediated RGC dysfunction, glial reactivity, and cellular death.
Factors influencing arteriovenous fistulas: A multicenter study

In this cross‐sectional, descriptive study, the arteriovenous fistulas (AVF) of HD patients were evaluated by physical examination and factors associated with AVF function and longevity were investigated. Data were collected using a patient information form and the Arteriovenous Fistula Assessment Scale (AVF‐AS). The study population included 279 patients under chronic HD treatment. Their mean age was 61.14 ± 14.00 years and 58.6% were men. Age, AVF location, and number of AVFs created were identified as factors associated with AVF needle entry site and flow problems, stenosis, and risk of developing ischemic complications (p = 0.005, p = 0.000, p = 0.006, respectively). AVF dysfunction adversely affected HD pump speed (p = 0.000) and HD adequacy (p = 0.000). It was determined that gender, AVF location, last AVF duration, and total number of previous AVF were identified as the risk of AVF complications. The results of this study revealed that regular follow‐up and evaluation are needed to minimize the risk of dysfunction and failure due to AVF complications.
Biocompatible and photocrosslinkable poly(ethylene glycol)/keratin biocomposite hydrogels

Abstract A biocompatible hydrogel is ideal for tissue engineering and regeneration. In this study, methacrylated keratin (KerMA) was synthesized for the first time and then blended with poly(ethylene glycol dimethacrylate) (PEGDMA) to form hydrogel through photocrosslinking. The chemical structure, gelation time, swelling behavior, hydrophilicity, cytotoxicity, and 3D printability of PEGDMA/KerMA hydrogels were characterized and exploited. The PEGDMA/KerMA hydrogels performed good cytocompatibility, providing potential applications for tissue engineering and regenerative medicine.
Isolation and Characterization of a Lytic Staphylococcus aureus Phage WV against Staphylococcus aureus Biofilm

Background: Staphylococcus aureus is a Gram-positive, pathogenic bacterium that causes a wide range of symptoms in humans and can form biofilm, which is a multicellular community of microorganisms that attaches to nonbiological and biological surfaces. Methods: Here, we aimed to isolate and characterize an S. aureus phage and examine the bactericidal activity alone and in conjunction with streptomycin treatment. Results: We isolated a virulent phage, WV, from a slaughterhouse in Jiangsu, China. This strain belonged to the family Myoviridae and presented a genome size of 141,342 bp. The optimal pH of the preservation buffer was 6–7, optimal growth temperature was 37°C, and optimal multiplicity of infection was 0.01. Phage WV can sterilize most clinical strains of S. aureus that had been isolated from clinical patients in the First People’s Hospital of the Yunnan Province. Against low-concentration S. aureus culture, streptomycin demonstrated a greater antibiofilm effect than that of phage WV. By contrast, in high-concentration S. aureus culture, phage WV demonstrated greater antibiofilm effect than that of streptomycin. The use of phage WV and streptomycin together had a substantially greater overall antibiofilm effect than that achieved using either component alone. Conclusion: This study provides strong evidence for the effectiveness of phage application for the reduction of S. aureus biofilm growth and suggests that phages can be considered as a viable alternative to antibiotics in clinical settings.
Effect of clear speech intervention program on speech intelligibility in persons with idiopathic Parkinson’s disease: A pilot study

Abstract Purpose: This preliminary study was designed to assess the feasibility of Clear Speech Intervention Program (CSIP) on improving speech intelligibility of persons with idiopathic Parkinson’s disease (PD). Clear Speech is a behavioural approach which focuses on improving speech intelligibility of persons with PD within a relatively short frame of time. Method: A single group pretest-posttest design was used to assess the effectiveness of CSIP. Fifteen adults diagnosed with PD participated in 8 sessions of CSIP with each session lasting 45–50 minutes over a period of 4 weeks in South Korea. All the data were collected in a quiet room with an ambient noise level of equal to or less than 40 dB SPL. Data on acoustical/aerodynamic, auditory-perceptual, and self-perceptual measures were obtained pre- and post-intervention. The Phonatory Aerodynamic System (PAS) was used to analyse acoustical/aerodynamic data. Result: The acoustical/aerodynamic assessment revealed statistically significant (p < 0.01) improvements in habitual pitch, pitch range, speaking rate, and number of respirations. In the auditory-perceptual assessment, statistically significant (p < 0.01) improvements were observed in overall speech intelligibility, tone, intonation, intensity, and articulation. Medium to large effect sizes were observed for significant results. The Pearson correlation analysis showed significant (p < 0.01) and strong correlation between articulation and speech intelligibility. Conclusion: The preliminary data has provided promising results and warrants future large-scale studies with suitable controls. Additionally, future research must focus on investigating long term effects of the intervention.
SERONEGATIVE CELIAC DISEASE IN BRAZILIAN PATIENTS: A SERIES OF CASES.

BACKGROUND Celiac disease (CD) is an autoimmune disease characterized by immune reaction mostly to wheat gluten. The diagnosis is based on clinical, serological and histological findings in patients ingesting gluten. Cases that the clinical profile indicates CD and the autoantibodies are negative bring so a dilemma for the professional, as the risk of missed the diagnosis or a delay at the same. OBJECTIVE To show the importance of correct diagnosis of cases with seronegative celiac disease (SNCD). METHODS Ten cases of SNCD Brazilian patients were retrospectively studied (2013 to 2019). Data of clinical complaints, autoantibodies, IgA serum levels, histological findings and HLA-DQ2/DQ-8 were compiled. Dual-X densitometry, delay at diagnosis, previous autoimmune diseases and family history of CD were also checked. RESULTS All SNCD patients presented clinical symptoms of CD, with confirmed diagnosis by histological findings of the duodenal mucosa and HLA-DQ2 and/or HLA-DQ8 positivity. All patients had normal IgA levels and negative autoantibodies (IgA-anti-transglutaminase and anti-endomysial). Dual-X densitometry detected osteopenia in two women and osteoporosis in two males, all with low levels of vitamin D. Delay diagnostic ranged from 1 to 19 years. Familiar occurrence of CD was reported in 40% of the cases. After one year of gluten-free diet, eight patients refer improve of symptoms, while duodenal biopsies, done in five cases, showed histological improvement. CONCLUSION Patients who demonstrate the clinical profile of celiac disease with negative serology and normal levels of IgA, especially those who have family members with celiac disease, should be submitted to duodenal biopsies to look for histological findings.
High Triglyceride Glucose Index Is Associated with Poor Outcomes in Ischemic Stroke Patients after Reperfusion Therapy

Introduction: The triglyceride glucose index (TyG index) is a simple and reliable surrogate marker of insulin resistance (IR) that can predict functional outcomes and mortality after acute ischemic stroke (AIS). However, it is unclear whether the TyG index is associated with functional outcomes in patients with stroke who receive reperfusion therapy. Thus, we aimed to explore the prognostic value of the TyG index for the clinical outcomes of patients with AIS who underwent reperfusion therapy. Methods: We retrospectively assessed patients with AIS, with occlusion of either the middle cerebral artery or internal carotid artery, who were evaluated using multiphase computed tomography angiography (mCTA) and received reperfusion therapy. The TyG index was calculated as “ln [fasting glucose level (mg/dL) × triglyceride level (mg/dL)]/2. ” Collateral status was evaluated using mCTA based on the University of Calgary Scale. Clinical outcomes included 3-month functional outcomes, early neurological deterioration, recanalization status, and hemorrhagic transformation. Results: In all, 183 subjects (age 69.5 ± 12.4 years; men, 59.0%) were enrolled. The median initial National Institutes of Health Stroke Scale score was 15.0 (interquartile range [IQR] 11–18). The median TyG index was 4.8 (IQR, 4.6–5.1), and 158 patients had TyG levels >4.49, which represents the presence of IR. On univariate analysis, a higher TyG index was associated with both early neurological deterioration (18.4 vs. 0.0%, p = 0.041) and a 3-month poor functional outcome (mRS3-6) (61.4 vs. 32.0%, p = 0.011). After adjusting for multiple variables, including age, sex, type of reperfusion therapy, recanalization status, initial stroke severity, type of stroke, and history of hypertension and diabetes, high TyG index remained an independent predictor of a poor 3-month functional outcome (adjusted OR, 5.22; p = 0.014). However, TyG levels were not significantly associated with collateral status (p = 0.756). Conclusions: IR, represented by a high TyG index, may predict poor 3-month functional outcomes in patients with AIS who undergo reperfusion therapy.