Nanos3 knockout pigs to model transplantation and reconstruction of the germline

Dear Editor, The anatomy and physiological characteristics of pigs are closer to those of humans than those of other non-primate animals. Therefore, pigs are considered excellent models for xenotransplantation and disease modelling. Several pig models of human diseases have been developed using gene editing technology. However, some of these diseases are too severe to permit the survival and reproduction of pig models. Therefore, new methods need to be developed to preserve these valuable germlines and genetic resources. Spermatogonial stem cell transplantation (SSCT) is one method to protect the germplasm via germline transfer between male donors and recipients. Primor germ cells (PGCs) comprise the initial progenitors of the germ cell lineage during development. After a series of complex developmental processes, including epigenetic reprogramming and meiosis, PGCs can develop into sperm cells and oocytes in male and female embryos, respectively. Genes such as Nanos3, Tial and Dnd1 play important roles in the development of PGCs during their differentiation. After Nanos3 is deleted in mice, PGCs undergo apoptosis during migration, resulting in the complete loss of male and female germ cells. Histological findings revealed no germ cells in the testes or ovaries of Nanos3 pigs recently created by chimeric embryo injections. To improve upon chimeric embryo injections, which might yield mosaic pig models, the present study used somatic cell nuclear transfer-based cloning. The construction of non-mosaic, germline knockout Nanos3 / pigs with stable genotypes and phenotypes would be essential for germplasm preservation work. Here, we constructed a Nanos3 / pig by somatic cell nuclear transfer (Figure 1A). We initially constructed a Nanos3 knockout vector (Figure 1B) and cotransfected cultured primary pig embryonic fibroblasts (PEFs) with Cas9-GFP and an sgRNA for Nanos3. We obtained the male cell lines, Nanos3 / #7 and Nanos3 / #1, and a female cell line, Nanos3 / #51 (Figure 1C). Sanger sequencing of the PCR products of these knockout cell lines confirmed the correct genotype (Figure 1D). We then performed somatic cell nuclear transfer and transplanted 4156 reconstructed embryos into 23 surrogates, and 10 were detected using B-US between 28 and 32 days later. Finally, 18 male and 10 female cloned piglets were born (Figure 1C and Figure S1A). Genotyping of the ear tissues using PCR showed that Nanos3 was knocked out in all neonatal piglets (Figure 1E). The cloned piglets were raised in a clean, standard environment (Figure S1B). Statistical analysis showed that >70% of the cloned piglets survived for at least 200 days (Figure S1C). These data showed that somatic cell nuclear transfer led to the creation of stable model pigs with complete, constitutive and germline Nanos3 knockout. Next, we analysed the reproductive system of the Nanos3 / pigs. We stained the testes of Nanos3 / male pigs with haematoxylin and eosin (HE) at the ages of 0 days, 3, and 6 months, and found that spermatogonial stem cells and sperm could not be produced (Figure S1D and Figure 1H). Staining ovarian tissues from Nanos3 / pigs with HE revealed no primary and secondary follicles, or corpus luteum structures at 0 and 3 months (Figure S1E), and no follicle structures and oocytes at 6 months (Figure 1L). We then examined the protein expression of NANOS3 (Figure S1F), DAZL (Figure S1G), and DDX4 (Figure S1H) to confirm the presence of rare germ cells in the testes of Nanos3 / pigs, but no germ cells were detected. We also examined the cell division and differentiation markers (RD51, γH2AX, and CYP3) for spermatogonial stem cells in 3-month-old WT testes (Figure 1I–K), but no such cells were detected in Nanos3 / pigs. These findings indicated that Nanos3 knockout completely disrupted the formation and meiosis of spermatogonia in Nanos3 / pig testes. Moreover, DAZL, DDX4, or NANOG signals were undetectable in newborn and 3-month-old female Nanos3 / pigs, indicating the complete absence of female germ cells (Figure S1I–K and Figure 1M–O). We further analysed the expression of genes related to the proliferation, differentiation, and meiosis of spermatogonial stem cells in testes from Nanos3 / pigs (Figure 1F and Figure S1L). The expression of these genes was significantly decreased in Nanos3 / compared with wild-type (WT) pigs. The expression of germ cell-specific, follicle developmentand meiosis-associated genes, as well as the ZP3 gene associated with zona pellucida development was substantially lower in the ovaries of Nanos3 / , compared with WT pigs (Figures 1G and S1M). Body weight (Figure 1P,Q), and levels of testosterone, estradiol, prolactin, luteinizing and follicle-stimulating hormones (Figure 1R,S) did not significantly differ between WT and Nanos3 / pigs. Taken together, these data showed that Nanos3 knockout specifically disrupted the survival, proliferation, and differentiation of germ cells, which did not affect the overall growth and sex hormones of the genetically engineered pigs. Therefore, Nanos3 / pigs can theoretically be used as recipients for SSCT to perpetuate exogenous germlines. Received: 26 February 2023 Accepted: 15 March 2023