Miracle-dz/UniFAR-FTUs huggingface dataset:
A Phenotypic Profile of the Candidaalbicans Regulatory Network

Candida albicans is a normal resident of the gastrointestinal tract and also the most prevalent fungal pathogen of humans. It last shared a common ancestor with the model yeast Saccharomyces cerevisiae over 300 million years ago. We describe a collection of 143 genetically matched strains of C. albicans , each of which has been deleted for a specific transcriptional regulator. This collection represents a large fraction of the non-essential transcription circuitry. A phenotypic profile for each mutant was developed using a screen of 55 growth conditions. The results identify the biological roles of many individual transcriptional regulators; for many, this work represents the first description of their functions. For example, a quarter of the strains showed altered colony formation, a phenotype reflecting transitions among yeast, pseudohyphal, and hyphal cell forms. These transitions, which have been closely linked to pathogenesis, have been extensively studied, yet our work nearly doubles the number of transcriptional regulators known to influence them. As a second example, nearly a quarter of the knockout strains affected sensitivity to commonly used antifungal drugs; although a few transcriptional regulators have previously been implicated in susceptibility to these drugs, our work indicates many additional mechanisms of sensitivity and resistance. Finally, our results inform how transcriptional networks evolve. Comparison with the existing S. cerevisiae data (supplemented by additional S. cerevisiae experiments reported here) allows the first systematic analysis of phenotypic conservation by orthologous transcriptional regulators over a large evolutionary distance. We find that, despite the many specific wiring changes documented between these species, the general phenotypes of orthologous transcriptional regulator knockouts are largely conserved. These observations support the idea that many wiring changes affect the detailed architecture of the circuit, but not its overall output. to assign groups of regulators to specific biological roles, many of which are relevant to pathogenesis. For example, we identified groups of regulators that influenced sensitivity to antifungal drugs, the ability to acquire iron (a challenge for organisms in a human host), and the ability to form complex multi-cellular colonies. Our results also allow us to analyze how the phenotypes associated with transcriptional regulators change as organisms diverge. A comparison of C. albicans data with that from the well-characterized yeast S. cerevisiae revealed strong phenotypic conservation be- tween related transcriptional regulators, despite the more than 300 million years which separate the species.
Tracing the Development of Reflective Competence in Multi-Sited ITE Involving Telecollaboration: Empirical Findings and Pre-Service Teachers' Promise for Effective Practice and Innovation

This chapter illustrates the process and outcomes of developing reflective competence according to three preservice teachers who participated in a multi-sited learning environment, involving integrated telecol-laboration. The learning environment fused together university, virtual and school sites. An ethnographic multiple case study with quantitative measurements was employed to analyse the year-long trajectory of the preservice teachers’ learning to reflect. We gathered audio-visual data from beginning to end of the year, indicating participants’ growing ability to reflect on their own practices, and leading cognitive change and transformation of classroom practices. By tracing multimodal interactions sequentially, we were able to extract guidelines for creating useful collaborative artifact ecologies in Initial Teacher Education and discuss the relationship between reflection, cognitive development and different personality traits. Based on our findings, we empirically substantiate the argument that reflective competence can indeed trigger transformation in Initial Teacher Education.
QR BASED AADHAR ELECTRONIC VOTING SYSTEM WITH BLOCK CHAIN TECHNOLOGY USING INTERNET OF THINGS

— This system allows people to vote electronically in public elections with security system using Block chain technology . Progressively the application of scientific knowledge for the practical purpose within the idea helps many folks live. This methodology set out an Associate in legal organization for native election on blockchain knowledge dealing with applied sciences and Aadhar affirmation. The fact of being free from threat and dispositive may be a danger from extensive election with this prevailing methodology. A number of issues that may persist within the prevailing methodology are with a corporation, that has management over the information. It can be done to meddle with the information for a good time. The projected methodology is principally designed for our native supported Aadhar affirmation. To make sure a lot of security, fingerprint of elector is employed. A Single system will act as registering module and voting module. The system will act as registering module on activating switch by the super admin. For registering module, the QR code of Aadhar is scanned (which is brought by the use) followed by the fingerprint verification. The system permits the elector to vote through his fingerprint when they cast their vote, block chain technology comes into existence that is integrated within the machine. Each vote is added into each block encrypted by 256-bit SHA hash codes, the hashed block cannot be tampered by any individual as more security is added to the system. By adopting Blockchain within the distribution of information will scale back one in every of the cheating sources of database manipulation.
Plasma Lipoprotein Profile in Newly Hatched Chicks Following in Ovo Ghrelin Administration

Objective: The aim of this study was to investigation on effects of in ovo administrated exogenous ghrelin on plasma lipid profile in hatched broiler chicks. Methodology: 250 eggs were obtained from commercial broiler breeder (Ross 308 strain) farm. the eggs were divided into five experimental groups; T1 or eggs without any injection (control), T2 or eggs in ovo injected with 50ng ghrelin at embryonic day-5, T3 or eggs in ovo injected with 100ng ghrelin at embryonic day-5, T4 or eggs in ovo injected with 50ng ghrelin at embryonic day-10, and T5 or eggs in ovo injected with 100ng ghrelin at embryonic day-10. Similar in ovo experiments were done for all of injected groups on day-5 or -10. At end of incubation, blood samples from each group were collected following chick decapitation and analyzed for determination of plasma lipoprotein concentrations. Results: Exogenous ghrelin administration at different embryonic days couldn’t have any considerable effect on low density lipoprotein (LDL-C), very low density lipoprotein (VLDL-C) or high density lipoprotein (HDL)/LDL-C. Plasma HDL-C concentration had increase follow in ovo injection of 100ng ghrelin at day-5 (P<0.01) and had slight increase at day-10 (group T3 and T5). Conclusion: As conclusion, in ovo administration of 100ng ghrelin at embryonic day-5 could elevate plasma HDL-C concentrations of newly-hatched chicks without any significant effect on LDL-C, VLDL or HDL-LDL ratio.
The evolution of giant molecular clouds

We discuss the origin, lifetime, destruction, spatial distribution and relation to star formation of giant molecular clouds. A coagulation model including the effects of spiral density wave shocks is described. We explore implications for CO observations of external galaxies. The collective effects of OB star winds and supernova remnants in disrupting clouds are considered.
Related Party Transactions, Family Firms and Firm Performance Empirical Evidence From Turkey

The objective of this study is to examine the relationship between related party transactions and firm performance in the presence of family ownership. The study used data of 714 public listed firms in Istanbul stock exchange for the period of 2011-2015. Utilizing regression analysis of 714 Turkish listed firms, this study shows that related party transaction has a negative influence on firm performance. In addition, the study shows that this association is stronger in the presence of family ownership. The results proposed that a related party transaction is practiced by family firms to expropriate minority shareholders rights. The result is consistent with entrenchment hypothesis and tunneling.
Tit le A mechanism of membrane topology remodeling and variat ion in cell morphology ( 膜トポロジー変換と細胞形態の多様性を生み出す機構に ついて )

The remodeling of plasma membrane topology is a fundamental process in every living organism. The most well known example of membrane topological change is the terminal phase of cytokinesis, when one cell divides and gives rise to two daughter cells. During this process, single plasma membrane, which is a closed surface, undergoes remodeling of topology and finally . converts into two plasma membranes, each of them forming individual closed surface. The plasma membrane topology remodeling also plays an important role during development of epithelial tubular organs, such as the vascular system in vertebrates and the tracheal system in insects. Tubulognesis of these organs involves connection of tubes at their termini, as observed in the anastomosis formation between the capillary veins and arteries. Since each tube are closed at the terminus before the connection, cells at the tip of tubular branch remodel plasma membrane topology. After the initial contact between the tip cells, each of them converts into torus topology, a doughnut-like shape, thus the tubes are connected through the hollow in the cell body (Kamei et aI., 2006, Samakovlis et aI., 1996b). Prior to the cell topology change in the epithelial tube connection, vesicles assemble at the future hollow. This assembly is reported in both the vertebrate and the insect system, and it is hypothesized that this event is playing an essential role for
Algorithms for the analysis of sleep signals

In the next chapter a novel method for analyzing long term respiratory recordings will be presented, namely an algorithm built in order to extract the moments of onset of inspiration and expiration from long duration respiratory signals. The algorithm is based on artiﬁcial neural networks, more precisely, multilayer feed-forward networks, using a back propagation algorithm to learn a desired behavior. The present chapter will then focus on introducing the theoretical basis of artiﬁcial neural networks as well as some practical points of implementing such algorithms.
EFA Multidimensional measure of aggression in adolescents: Croatian validation of the Peer Conflict

results Both results of EFA and CFA support a proposed fourfactor model of the instrument. Reliabilities of the instrument’s scales were acceptable. The measurement invariance across gender was established. In order to analyse the construct validity of the PCS, relations between aggression subtypes and the theoretically meaningful variable, i.e. anxiety, were assessed. Reactive relational aggression had the highest correlation with anxiety, while proactive overt aggression did not correlate significantly with anxiety. Furthermore, gender differences in aggression subtypes were assessed, and were in accordance with past research.
CROSS-COMPATIBILITY OF AERIAL AND TERRESTRIAL LIDAR FOR QUANTIFYING FOREST STRUCTURE

Author: Wagner, Franklin, W. MS Institution: Purdue University Degree Received: August 2019 Title: Cross-Compatibility of Aerial and Terrestrial Lidar for Quantifying Forest Structure Committee Chair: Brady Hardiman, Songlin Fei Forest canopies are a critical component of forest ecosystems as they influence many important functions. Specifically, the structure of forest canopies is a driver of the magnitude and rate of these functions. Therefore, being able to accurately measure canopy structure is crucial to ensure ecological models and forest management plans are as robust and efficient as possible. However, canopies are complex and dynamic entities and thus their structure can be challenging to accurately measure. Here we study the feasibility of using lidar to measure forest canopy structure across large spatial extents by investigating the compatibility of aerial and terrestrial lidar systems. Building on known structure-function relationships measured with terrestrial lidar, we establish grounds for scaling these relationships to the aerial scale. This would enable accurate measures of canopy structural complexity to be acquired at landscape and regional scales without the time and labor requirements of terrestrial data collection. Our results illustrate the potential for measures of canopy height, vegetation area, horizontal cover, and canopy roughness to be upscaled. Furthermore, we highlight the benefit of utilizing multivariate measures of canopy structure, and the capacity of lidar to identify forest structural types. Moving forward, lidar is a tool to be utilized in tandem with other technologies to best understand the spatial and temporal dynamics of forests and the influence of physical ecosystem structure.
Phylogenetic and Metabolic Diversity of Planctomycetes from Anaerobic, Sulﬁde- and Sulfur-Rich Zodletone Spring, Oklahoma (cid:1)

We investigated the phylogenetic diversity and metabolic capabilities of members of the phylum Planctomy- cetes in the anaerobic, sulﬁde-saturated sediments of a mesophilic spring (Zodletone Spring) in southwestern Oklahoma. Culture-independent analyses of 16S rRNA gene sequences generated using Planctomycetes -biased primer pairs suggested that an extremely diverse community of Planctomycetes is present at the spring. Although sequences that are phylogenetically afﬁliated with cultured heterotrophic Planctomycetes were identiﬁed, the majority of the sequences belonged to several globally distributed, as-yet-uncultured Planctomycetes lineages. Using complex organic media (aqueous extracts of the spring sediments and rumen ﬂuid), we isolated two novel strains that belonged to the Pirellula-Rhodopirellula-Blastopirellula clade within the Planctomycetes . The two strains had identical 16S rRNA gene sequences, and their closest relatives were isolates from Kiel Fjord (Germany), Keauhou Beach (HI), a marine aquarium, and tissues of marine organisms ( Aplysina sp. sponges and postlarvae of the giant tiger prawn Penaeus monodon ). The closest recognized cultured relative of strain Zi62 was Blastopirellula marina (93.9% sequence similarity). Detailed characterization of strain Zi62 revealed its ability to reduce elemental sulfur to sulﬁde under anaerobic conditions, as well as its ability to produce acids from sugars; both characteristics may potentially allow strain Zi62 to survive and grow in the anaerobic, sulﬁde- and sulfur-rich environment at the spring source. Overall, this work indicates that anaer- obic metabolic abilities are widely distributed among all major Planctomycetes lineages and suggests carbohydrate fermentation and sulfur reduction as possible mechanisms employed by heterotrophic Planctomycetes for growth and survival under anaerobic conditions.
TNF Receptor Associated Periodic Syndrome associated with gonosomal mosaicism of a novel 24 nucleotide TNFRSF1A deletion

in the next generation sequencing experiments reported other financial support or other benefits from commercial sources for the work reported any other financial interests that any of the may create a potential conflict of interest or the appearance of a conflict of interest with to the Abstract Objective: To investigate the molecular cause of persistent fevers in a patient returning from working overseas, in whom investigations for tropical diseases were negative. Methods: DNA was extracted from whole blood, , leukocyte subpopulations, saliva, hair root and sperm. The TNFRSF1A gene was analysed by polymerase chain reaction (PCR), allele specific (AS)-PCR, Sanger-sequencing and, next-generation sequencing. In silico molecular modelling was performed to predict the structural and functional consequences to the extracellular domain of the mutant TNFR1 protein. Results: Sanger sequencing corroborated by AS-PCR detected a novel in-frame deletion of 24 nucleotides (c.255_278del) in the TNFRSF1A gene which was subsequently confirmed by the next-generation sequencing methods (targeted sequencing and amplicon-based deep sequencing). The latter revealed variable frequency of the mutant allele among different cell lines including sperm, supporting the presence of gonosomal TNFRSF1A mosaicism. The patient had a complete response to treatment with IL-1 blockade, with resolution of symptoms and normalization of acute phase proteins.
A comparison of the performance of prediction techniques in curtailing uplink transmission and energy requirements in mobile free-viewpoint video applications

- The rapid deployment of multimedia services on mobile networks together with the increase in consumer demand for immersive entertainment have paved the way for innovative video representations. Amongst these new applications is free-viewpoint video (FVV), whereby a scene is captured by an array of cameras distributed around a site to allow the user to alter the viewing perspective on demand, creating a three-dimensional (3D) effect. The implementation on mobile infrastructures is however still hindered by intrinsic wireless limitations, such as bandwidth constraints and limited battery power. To this effect, this paper presents a solution that reduces the number of uplink requests performed by the mobile terminal through view prediction techniques. The implementation and performance of four distinct prediction algorithms in anticipating the next viewpoint request by a mobile user in a typical FVV system are compared and contrasted. Additionally, each solution removes the jitter experienced by the user whilst moving from a view pattern to another by allowing some hysterisis in the convergence signal. Thus, this technique enhances the performance of all the algorithms by taking into consideration the fact that the user adapts to the presented views and will react accordingly. Simulation results illustrate that an uplink transmission reduction of up to 96.7% can be achieved in a conventional FVV simulation scenario. Therefore, the application of prediction schemes can drastically reduce the mobile terminal’s power consumption and bandwidth resource requirements on the uplink channel.
Automated Classification of Web Pages for Identification of Suspicious Food Products – a Feasibility Study

eine falsch-positiv als auffällig klassifizierte Probe mit der SVM-Methode). Der Ansatz wird daher als vielversprechend angesehen, um die Lebensmittelkontrolle des Internethandels zu verbessern. Auf-grund der Komplexität der Prozesse könnten derartige Recherchen bei einer Zentralstelle für die Kontrolle des Internethandels angesiedelt sein, wobei darauf hingewiesen wird, dass neben den Softwarekenntnissen auch ein erhebliches Expertenwissen über die Produktbewertung erfor-derlich ist, um die Klassifizierungsverfahren zu trainieren.
Chimeric G,,/Gai2 Proteins Define Domains on G,, That Interact with Tubulin for P-Adrenergic Activation of Adenylyl Cyclase*

Previous studies have demonstrated that dimeric tu- bulin, associated with synaptic membrane, is capable of activating the G-proteins G, and Gail via transfer of GTP. To clarify the mechanism of intracellular interaction between tubulin and G,, as it refers to adenylyl cy- clase activation, wild type and chimeric GJG, pro- teins were transiently overexpressed in COS 1 cells. Effects of tubulin dimers with guanosine 5’-(P,y-imi- do)triphosphate (Gpp(NH)p) bound (tubulin-Gpp(NH)p) or Gpp(NH)p withlwithout isoproterenol on adenylyl cyclase were assessed P-adrenergic agonist stimulatory In 1 the chimera G,i(sq)lB (Gai2 G, 62-394 p(NH)p than Gpp(NH)p in the presence
Hydrogen sensor based on field effect transistor with C–Pd layer

ISFET (Ion Sensitive Field Eﬀect Transistors) microsensors are widely used for pH measurements as well as analytical and biomedical applications. At the same time, ISFET is a good candidate for testing various materials for their applications in sensitive membranes. For example, hydrogen sensitive carbonaceous ﬁlms containing Pd nanocrystallites (C–Pd) make this material very interesting for sensor applications. A cost eﬀective silicon technology was selected to fabricate n-channel transistors. The structures were coupled to specially designed double-sided PCB (Printed Circuit Board) holder. The holder enables assembly of the structure as part of an automatic stand. The last step of production of MIS structures was deposition of the C–Pd layer. The C–Pd ﬁlms were fabricated by the Physical Vapor Deposition (PVD) method in which C60 and palladium acetate were evaporated. Electrical resistance of structures with C–Pd ﬁlms was measured during their interaction with hydrogen. Finally, a new type of highly sensitive FET hydrogen sensor with C–Pd layer was demonstrated and characterized.
Supplementation with EPA or ﬁsh oil for 11 months lowers circulating lipids, but does not delay the onset of diabetes in UC Davis-type 2 diabetes mellitus rats

EPA or ﬁsh oil supplementation has been suggested as treatments for the prevention of type 2 diabetes mellitus (T2DM) due to their lipid-lowering and potential insulin-sensitising effects. We investigated the effects of supplementation with EPA (1g/kg body weight per d) or ﬁsh oil (3g/kg body weight per d) on the age of onset of T2DM and circulating glucose, insulin, lipids, leptin and adiponectin in UC Davis (UCD)-T2DM rats. Animals were divided into three groups starting at 1month of age: control, EPA and ﬁsh oil. All the animals were followed until diabetes onset or for up to 12 months of age. Monthly fasting blood samples were collected for the measurement of glucose, lipids, hormones and C-reactive protein (CRP). Neither EPA nor ﬁsh oil delayed the onset of T2DM or altered fasting plasma glucose, insulin, CRP, adiponectin or leptin concentrations. The groups did not differ in energy intake or body weight. Fish oil treatment lowered fasting plasma TAG concentrations by 39 ( SD 7)% ( P , 0·001) and EPA lowered fasting plasma NEFA concentrations by 23 ( SD 5)% ( P , 0·05) at 4 months of age compared with the control group. EPA and ﬁsh oil lowered fasting plasma cholesterol concentrations at 4 months of age by 19 ( SD 4) and 22 ( SD 4)% compared with the control group, respectively (both P , 0·01). In conclusion, EPA and ﬁsh oil supplementation lowers circulating lipid concentrations, but does not delay the onset of T2DM in UCD-T2DM rats.
STUDY ON INTERACTION OF IMATINIB IN LUNG CANCER WITH TUMOR ASSOCIATED MACROPHAGES

: Introduction: Imatinib is a tyrosine kinase inhibitor and firstly used for Philadelphia chromosome-positive (Ph + ) chronic myelogenous leukemia (CML). It has also been found that Imatinib has therapeutic effect on several solid tumors. Aims and objectives: The basic aim of the study is to find the interaction of Imatinib in lung cancer with tumor associated macrophages. Methodology of the study: This study was conducted at DHQ hospital Khanewal during June 2018 to August 2018 with the permission of ethical committee of hospital. Imatinib mesylate was obtained from Sigma- Aldrich and its solutions were prepared at the stock concentration of 50 mM (DMSO). Recombinant murine IL-13, IL-4 and M-CSF were purchased from PeproTech. LPS was also purchased from Sigma-Aldrich. Total RNA from RAW264.7 cells and BMDMs was isolated using Trizol (Invitrogen, CA, USA), and cDNA was synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix. Results: Given that STAT6 plays a key role in IL-13 or IL-4 induced macrophage M2-like polarization, we assessed whether STAT6 is involved in Imatinib inhibited M2 polarization. IL- 13 markedly triggered the phosphorylation of STAT6 in 30 min, and reached a peak in 1 h, which was significantly restrained by Imatinib. Conclusions: It is concluded that Imatinib inhibits macrophage M2-like polarization both in vitro and in vivo which contributes to its anti-metastatic function in lung cancer.