Miracle-dz/UniFAR-FTUs huggingface dataset:
Running title: PBF is required for LNCaP cell growth and invasion Pituitary tumor transforming gene binding factor (PBF) is required for androgen-induced prostate cancer proliferation and invasion

Pituitary tumor transforming gene binding factor (PBF) is a proto-oncogene that plays a role in many cancers; however, its involvement in prostate cancer (PCa) remains unclear. Here, we examined PBF expression in clinical specimens and investigated its regulation and function in human PCa cell lines. Immunohistochemical staining of patient tissues revealed higher PBF expression in PCa than in benign prostatic hyperplasia or adjacent normal prostate specimens. In LNCaP and 22Rv1 cells, PBF expression was upregulated by androgen treatment in a manner partially blocked by the androgen receptor (AR) antagonist bicalutamide. We identified a novel androgen response element in the PBF gene promoter and demonstrated its functional relevance using luciferase reporter assays. Androgen treatment of LNCaP cells induced binding between the endogenous AR and the androgen response element in PBF, as measured by chromatin immunoprecipitation assays. Finally, RNA interference of PBF expression significantly reduced androgeninduced LNCaP cell growth and invasion. Thus, PBF is a novel AR target gene and plays a role in androgeninduced proliferation and metastatic functions in PCa cells.
STUDY OF ANTIOXIDANT ACTIVITY AND ESSENTIAL OILS COMPOSITION FROM THE FLOWERS AND FRUITS OF CRYPTOCARYA STOCKSII MEISN

: The aim of this study was to investigate the flowers and fruits essential oil composition and antioxidant activity of Cryptocarya stocksii, an endemic and vulnerable tree grows in the Western Ghats of India. The essential oils were obtained by hydrodistillation using a Clevenger apparatus and analyzed by GC/MS. GC-MS analysis of essential oil of flower and fruit revealed the presence of individual 24 different compounds corresponding to 99.87% and 99.99% of the total oil respectively. The flower and fruit oil were dominated by α-Pinene (39.42% and 8.84%), β-Pinene (17.96% and 4.87%), 3-Carene (29.52% and 1.92%) and Longifolene (6.53% and 19.12%) respectively. Antioxidant activities were determined by four different test systems, namely, DPPH, FRAP, H 2 O 2 , and anti-lipid peroxidation assay and result found to be dose dependent manner. This work represents the first study of the chemical composition and anti oxidant of essential oils from flowers and fruits of C. stocksii.
Endothelial Shc Regulates Arteriogenesis Through Dual Control of Arterial Specification and Inflammation via the Notch and NF-  B

Rationale: Arteriogenesis, the shear stress-driven remodeling of collateral arteries, is critical in restoring blood flow to ischemic tissue following a vascular occlusion. Our previous work has shown that the adaptor protein Shc mediates endothelial responses to shear stress in vitro . Objective: To examine the role of the adaptor protein Shc in arteriogenesis and endothelial-dependent responses to shear stress in vivo. Methods and Results: Conditional knockout mice in which Shc is deleted from endothelial cells were subjected to femoral artery ligation. Hindlimb perfusion recovery was attenuated in Shc conditional knockout mice compared to littermate controls. Reduced perfusion was associated with blunted collateral remodeling and reduced capillary density. Bone marrow transplantation experiments revealed that endothelial Shc is required for perfusion recovery as loss of Shc in bone marrow-derived hematopoietic cells had no effect on recovery. Mechanistically, Shc deficiency resulted in impaired activation of the NF- B-dependent inflammatory pathway and reduced CD45 + cell infiltration. Unexpectedly, Shc was required for arterial specification of the remodeling arteriole by mediating upregulation of the arterial endothelial cell marker ephrinB2 and activation of the Notch pathway. In vitro experiments confirmed that Shc was required for shear stress-induced activation of the Notch pathway and downstream arterial specification through a mechanism that involves upregulation of Notch ligands Delta-like 1 and Delta-like 4. Conclusions: Shc mediates activation of two key signaling pathways that are critical for inflammation and arterial specification; collectively, these pathways contribute to arteriogenesis and recovery of blood we introduce an alternative model in which shear stress directly activates the Notch pathway pre-existing collateral arterioles and in upregulation of target during arteriogenic and ECs We show that activation of the Notch pathway by shear stress requires Shc, which mediates signal transduction downstream of the mechanosensory complex of PECAM-1, VE-Cadherin and VEGFR2 integrins 15 . Shear-stress induced activation the Notch pathway facilitates arterial specification of collateral ECs as they remodel into high-flow carrying arterioles. The mechanism to explain how Notch is activated by shear stress is currently not well understood, although our work and work of others suggests that shear stress induces expression of Notch ligands Delta-like 1 and/or Delta-like 4, which in turn activate Notch in trans through the the and left side, from the m. adductor and m. gastrocnemius . The plasma membrane of capillary ECs in tissue sections was labeled with Alexa 568-IsolectinB4 (Invitrogen, CA). Micrographs were obtained with the Nikon fluorescence microscope using a 20x objective lens. Digitized images were analyzed with an image analysis package, Image J. The total number of capillaries was counted on 5 random optical fields each mouse. Results capillaries
PEDAGOGICAL CONCEPT AND ITS FEATURES, SOCIAL WORK AND LINGUOLOGY

The scientific research is devoted to the investigation of the cognitive-communicative approach in the foreign language teaching. It is emphasized that for providing language proficiency at the level that would allow the use of the foreign language in professional activities, students’ positive motivation must be paid great attention as the incontestable factor in successful learning. Teaching a foreign language based on cognitive communicative technologies is one of the most dynamically developing areas in the methodology, whereas the leading role in its implementation is played by the communicative principle and the principle of consciousness, being the base of forming an adequate understanding of speech phenomena and skills, as well as the ability to apply them in real communication. The cognitive-communicative approach is understood as such organization of the educational process, the basis of which is students’ communicative interaction, which provides their participation as an object and subject of cognitive activity.
IoT Data based Predictive Modeling for Energy Usage of Appliances in Smart Home

Internet of things (IoT) has emerged as the new trends in the wireless technology in last few years. This new area has greatly influenced the working of humans. IoT has applications in every domains of human being. The growth rate of IoT devices is exponential due to its wide applicability. Therefore, the data generated by these devices is huge and contains high variability. Such a huge amount of data needs to be modeled precisely. The effectiveness of the IoT applications lies in the preciseness of the data represented by the models. Predictive analysis helps business analysts to build models to predict trends, make tradeoff decisions, and model the real world for decision making support system. This paper presents the study on various models used for IoT data analytics. Various predictive models such as Multiple Linear Regression (LR), Support Vector Machine for regression (SVR), Random Forest (RF), Gradient Boosting Machine (GBM) and extreme Gradient Boosting Machine (XGBM) are applied on the sensor data collected from Smart Home. The comparative results produced by these models have been analyzed with reference to energy consumption and prediction. The implementation of the models is carried out on R language. The results show that XGBM model perform better based on RMSE, R-squared and MAE for given data set. It has less RMSE and high R-squared which indicates it has captured high variability in the data.
Microwave Photonics and Fiber-Wireless Interface – a Principal Approaches to Design Millimeter-Wave Network Equipment for the Next-Generation Wireless Communication Systems

—Significant milestones in the way to transform 4G LTE to 5G NR are analyzed in detail. It is shown that a number of the important tasks can be successfully implemented by a microwave photonic or/and millimeter-wave photonic approach to design network and terminal radio equipment. Short descriptions of microwave photonics as an interdisciplinary scientific and technological field, classification of the key elements and devices, and typical layouts of microwave-photonics-based radio-frequency transmitter and receiver units are highlighted. A version of cost-and power-efficient 5G remote station based on millimeter-wave photonic approach is proposed and discussed. As an application example, 5G fiber-wireless network for a regional-level area is design.
Nitric oxide inhibits Ca mobilization through cADP-ribose signaling in coronary arterial smooth muscle cells

Yu, Jiang-Zhou, David X. Zhang, Ai-Ping Zou, William B. Campbell, and Pin-Lan Li. Nitric oxide inhibits Ca mobilization through cADP-ribose signaling in coronary arterial smooth muscle cells. Am J Physiol Heart Circ Physiol 279: H873–H881, 2000. —The present study was designed to determine whether the cADP-ribose-mediated Ca signaling is involved in the inhibitory effect of nitric oxide (NO) on intracellular Ca mobilization. With the use of fluorescent microscopic spectrometry, cADP-ribose-induced Ca release from sarcoplasmic reticulum (SR) of bovine coronary arterial smooth muscle cells (CASMCs) was determined. In the a-toxin-permeabilized primary cultures of CASMCs, cADP-ribose (5 mM) produced a rapid Ca release, which was completely blocked by pretreatment of cells with the cADP-ribose antagonist 8-bromo-cADP-ribose (8-BrcADPR). In intact fura 2-loaded CASMCs, 80 mM KCl was added to depolarize the cells and increase intracellular Ca concentration ([Ca]i). Sodium nitroprusside (SNP), an NO donor, produced a concentration-dependent inhibition of the KCl-induced increase in [Ca]i, but it had no effect on the U-46619-induced increase in [Ca]i. In the presence of 8-BrcADPR (100 mM) and ryanodine (10 mM), the inhibitory effect of SNP was markedly attenuated. HPLC analyses showed that CASMCs expressed the ADP-ribosyl cyclase activity, and SNP (1–100 mM) significantly reduced the ADP-ribosyl cyclase activity in a concentration-dependent manner. The effect of SNP was completely blocked by addition of 10 mM oxygenated hemoglobin. We conclude that ADP-ribosyl cyclase is present in CASMCs, and NO may decrease [Ca]i by inhibition of cADP-ribose-induced Ca mobilization.
Proteasome Inhibitors in the Treatment of Multiple Myeloma

Proteasome inhibitors have become indispensable in myeloma management in transplant eligible and ineligible patients in both induction and salvage settings. Excellent efficacy and a manageable toxicity profile haves made these agents the backbone of myeloma care. After the first in class drug bortezomib was approved, several newer agents have been developed. The parenteral second generation drug carfilzomib has less peripheral neuropathy incidence than bortezomib. Oral proteasome inhibitors such as ixazomib, oproxomib and marizomib attempt to overcome the administration difficulties of the parenteral agents and have a differentiated toxicity profile. This review will focus on the role of proteasome inhibitors in myeloma treatment.
Effectiveness of chlorhexidine / silver sulfadiazine catheters in the prevention of catheter-related bloodstream infections : A traditional meta analysis

[摘要] 目的 探讨氯己定/磺胺嘧啶银涂层预防导管相关性血流感染(CR BSI)的效果。 方法 计算机检索 Cochrane Library、Embase、Medline、CINAHL、Web of Science等数据库从建库至2019年3月有关CRBSI预防的临床随机 对照试验。 采用Cochrane偏倚风险评估工具评估纳入的研究,提取有效数据,采用95%置信区间(CI)计算集合风险比 率(RR),测量CRBSI千日率及细菌定植率。 以CRBSI千日率为首要结局,细菌定植率为次要结局,采用R3. 4. 1软件进行 Meta分析。 结果 共纳入17项研究,包括4892例患者。 氯己定/磺胺嘧啶银涂层导管的细菌定植率明显低于常规标准 导管(RR=0.55,95%CI: 0.45~0.68,P=0.02),但CRBSI千日率差异无统计学意义(RR=0.70,95%CI: 0.45~1.11,P=0.32)。 结论 与常规标准导管相比,集束化管理指导下使用氯己定/磺胺嘧啶银涂层导管可降低导管相关细菌定植率,但对 CRBSI千日率无影响。 [关键词] Meta分析;集束化管理;导管相关血流感染;导管定植氯己定/磺胺嘧啶银涂层 [中图分类号] R732.2 [文献标志码] A [文章编号] 0577-7402(2020)07-0751-06 [DOI] 10.11855/j.issn. 0577-7402.2020. 07.13
This is a repository copy of Prevalence of irritable bowel syndrome-type symptoms in patients with inflammatory bowel disease in remission: a systematic review

eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can’t change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/
Sahota, Opinder and Pulikottil-Jacob, Ruth and Marshall, Fiona and Montgomery, Alan and Tan, Wei and Sach, Tracey and Logan, Pip and Kendrick, Denise and Watson, Alison and Walker, Maria and Waring, Justin (2016) The Community In-reach Rehabilitation and Care

Objective: to compare the clinical and cost-effectiveness of a Community In-reach Rehabilitation and Care Transition (CIRACT) service with the traditional hospital-based rehabilitation (THB-Rehab) service. Design: pragmatic randomised controlled trial with an integral health economic study. Settings: large UK teaching hospital, with community follow-up. Subjects: frail older people aged 70 years and older admitted to hospital as an acute medical emergency. Measurements: Primary outcome: hospital length of stay; secondary outcomes: readmission, day 91-super spell bed days, functional ability, co-morbidity and health-related quality of life; cost-effectiveness analysis. Results: a total of 250 participants were randomised. There was no significant difference in length of stay between the CIRACT and THB-Rehab service (median 8 versus 9 days; geometric mean 7.8 versus 8.7 days, mean ratio 0.90, 95% confidence interval (CI) 0.74–1.10). Of the participants who were discharged from hospital, 17% and 13% were readmitted within 28 days from the CIRACT and THB-Rehab services, respectively (risk difference 3.8%, 95% CI −5.8% to 13.4%). There were no other significant differences in any of the other secondary outcomes between the two groups. The mean costs (including NHS and personal social service) of the CIRACT and THB-Rehab service were £3,744 and £3,603, respectively (mean cost difference £144; 95% CI −1,645 to 1,934). Conclusion: the CIRACT service does not reduce major hospital length of stay nor reduce short-term readmission rates, compared to the standard THB-Rehab service; however, a modest (<2.3 days) effect cannot be excluded. Further studies are necessary powered with larger sample sizes and cluster randomisation. Trial registration: ISRCTN 94393315, 25th April 2013
Mechanism for Immune Regulation Q T Cells: A Feedback a 24J a Activity of V Cytokine Secretion from and Cytolytic CD1d on Myeloid Dendritic Cells Stimulates

The precise immunologic functions of CD1d-restricted, CD161 1 AV24AJ18 (V a 24J a Q) T cells are not well deﬁned, although production of IL-4 has been suggested as important for priming Th2 responses. However, activation of human V a 24J a Q T cell clones by anti-CD3 resulted in the secretion of multiple cytokines notably important for the recruitment and differentiation of myeloid dendritic cells. Speciﬁc activation of V a 24J a Q T cells was CD1d restricted. Expression of CD1d was found on monocyte-derived dendritic cells in vitro, and immunohistochemical staining directly revealed CD1d preferentially expressed on dendritic cells in the paracortical T cell zones of lymph nodes. Moreover, myeloid dendritic cells both activated V a 24J a Q T cells and were susceptible to lysis by these same regulatory T cells. Because myeloid dendritic cells are a major source of IL-12 and control Th1 cell differentiation, their elimination by lysis is a mechanism for limiting the generation of Th1 cells and thus regulating Th1/Th2 responses. The Journal of Immunology, 2000, 165: 3756–3762.
HIV_A_266460 393..402

Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia Background: Highly active antiretroviral therapy has dramatically altered progression of HIV infection and significantly improved patients’ quality of life. However, drug resistance with consequent treatment failure raises the need for much more expensive and toxic secondline regimens. Thus, this study aimed at investigating the predictors of virologic failure among adults in Northeast Ethiopia. Methods: A retrospective cohort study was carried out among adults who started first-line antiretroviral treatment from September 2005 to January 2018. Data were collected from patients’ medical records, entered and validated using EpiData version 3.1 and then exported to SPSS version 20 for analysis. Binary logistic regression was carried out; odds ratio with 95% CI was used to identify covariates associated with virologic failure. Statistical significance was considered at p-value <0.05. Results: A total of 384 patients with mean age of 35.73±9.44 years were consecutively enrolled; of which, 213 (55.5%) were females, 255 (66.4%) had WHO clinical stage III/IV, and 130 (33.9%) had baseline CD4 count <100 cells/mm. Mean baseline CD4 count was 179 cells/mm (range: 2–853 cells), and 158 (41.1%) participants were on AZT/3TC/NVP. Virological failure was diagnosed among 61 (15.9%) patients. The mean time to virologic failure after initiation of ART was 63.80 months (range: 17–150 months). After adjusting for other confounders, risk of experiencing virologic failure was significantly associated with being divorced (AOR 3.40, 95% CI 1.20–9.59), being naïve to ART (AOR 2.55, 95% CI 1.23–5.28), low (<100) baseline CD4 count (AOR 2.39, 95% CI 1.03–5.54) and nonadherence (AOR 6.73, 95% CI 3.29–13.76). Conclusion: In this study, the prevalence of antiretroviral treatment failure was 15.9%. Being divorced, being naïve to antiretroviral therapy, low (<100 cells/mm) baseline CD4 count and nonadherence were found to be significant predictors of virologic failure. ART programs should focus on early HIV diagnosis and ART initiation as well as enhanced adherence support.
Enhanced Antibacterial Activity of Dermaseptin through Its Immobilization on Alginate Nanoparticles—Effects of Menthol and Lactic Acid on Its Potentialization

Dermaseptin B2 (DRS-B2) is an antimicrobial peptide secreted by Phyllomedusa bicolor, which is an Amazonian tree frog. Here, we show that the adsorption of DRS-B2 on alginate nanoparticles (Alg NPs) results in a formulation (Alg NPs + DRS-B2) with a remarkable antibacterial activity against Escherichia coli ATCC 8739 and E. coli 184 strains, which are sensitive and resistant, respectively, to colistin. The antibacterial activity, obtained with this new formulation, is higher than that obtained with DRS-B2 alone. Of note, the addition of lactic acid or menthol to this new formulation augments its antibacterial activity against the aforementioned Gram-negative bacilli. The safety of DRS-B2, and also that of the new formulation supplemented or not with a small molecule such as lactic acid or menthol has been proven on the human erythrocytes and the eukaryotic cell line types HT29 (human) and IPEC-1 (animal). Similarly, their stability was determined under the conditions mimicking the gastrointestinal tract with different conditions: pH, temperature, and the presence of digestive enzymes. Based on all the obtained data, we assume that these new formulations are promising and could be suggested, after in vivo approval and completing regulation aspects, as alternatives to antibiotics to fight infections caused by Gram-negative bacilli such as E. coli.
Innovative System for Evaluation and Rehabilitation of Human Imbalance.

Introduction: Mobile posturography is based on wearable inertial sensors; it allows to test static stability (static posturography) and gait disturbances. Aim: The aim of this work was to present the results of research on the innovative MEDIPOST system used for diagnosis and rehabilitation of balance disorders. Material and methods: Fourteen articles published in influenced foreign journals were presented and discussed. The deve-lopment and construction of the device was preceded by a literature review and methodological work. The Dizziness Handi-cap Inventory (DHI) questionnaire was translated and validated. The methodology of posturography with head movements with a frequency of 0.3 Hz was also developed in the group with chronic vestibular disorders. Simultaneous measurements were performed (static posturogrphy vs. MEDIPOST) in the CTSIB-M (Modified Clinical Test of Sensory Interaction in Balance) test in healthy subjects and patients with unilateral peripheral dysfunction. Results: In the posturography with head movements the improvement of sensitivity (67 to 74%) and specificity (65 to 71%) was noted. In the CTSIB-M test the intraclass correlation coefficients for both methods were 0.9. The greatest differences between examinations were observed for the mean angular velocity in the tests on the foam (trials no. 3 and 4), in particular on the foam with eyes closed (trial no. 4 - sensitivity 86.4%, specificity 87.7%). Two functional tests were analyzed: the Swap Seats test and the 360 degree turn test. In the former, the results are studied from 6 sensors - 86% of the true positives and 73% of the true negatives for the fall/ no-fall group classification. The second test differentiates people with vestibular impairment and healthy people. It can be analyzed with 1 (sensitivity 80%) and 6 sensors (sensitivity 86%, specificity 84%). Currently, the MEDIPOST device is in the development and certification phase.
Chemoprevention: Achievements and Future Perspectives

Cancer is the second deadliest disease in the world with very high potential of prevention. The past few decades have witnessed an increased interest in cancer chemoprevention among researchers, clinicians and the public because a successful prevention strategy could save millions of lives. This new paradigm has led to the critical evaluation of prospective chemopreventive agents including natural dietary agents, molecular targeted agents, vaccines etc. preclinically and clinically. Many dietary agents were accorded chemopreventive status based on epidemiological studies that linked their consumption to reduce cancer risk. Despite demonstrated effectiveness in preclinical and animal model studies, their safety with long-term use remains a major concern. Although more rigorous clinical trial protocols have been developed to assess candidates for chemoprevention, accrual, compliance, and retention in chemoprevention trials possess significant challenge because volunteers for such trials are healthy people with high risk of developing cancer. In this review, we critically review various preventive strategies, promising chemopreventive agents, challenges associated with successful chemoprevention and potential ways to overcome these obstacles. Dhaka Univ. J. Pharm. Sci. 20(3): 359-372, 2022 (June) Centennial Special Issue