Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of physical activity intervention on cognitive function in China: A cluster randomized trial

Current randomized trial evidence on the effect of physical activity (PA) intervention on cognitive function is scarce and mainly found in well‐educated populations, limiting its generalizability. Furthermore, the modification effect of education levels remains understudied. We conducted a cluster randomized trial to evaluate the effects of PA intervention on cognitive function in a general older population, and whether education modifies such effects.
Multiband s± Eliashberg theory and temperature-dependent spin-resonance energy in iron pnictide superconductors

The phenomenology of iron pnictide superconductors can be explained in the framework of a three-band s± wave Eliashberg theory with only two free parameters plus a feedback effect, i.e., the effect of the condensate on the antiferromagnetic spin fluctuations responsible for the superconductivity in these compounds. I have examined the experimental data of four materials, LaFeAsO1−xFx , SmFeAsO1−xFx, Ba1−xKxFe2As2, and Ba(FexCo1−x )2As2, and I have found that it is possible to reproduce the experimental critical temperature and gap values in a moderate strong-coupling regime, λtot ≈ 1.7 − 2.0
Warm Core Structures in Organized Cloud Clusters Developing or Not Developing into Tropical Storms Observed by the Advanced Microwave Sounding Unit

Abstract The temperature profiles of organized cloud clusters developing or not developing (nondeveloping) into tropical storms (TSs; maximum surface wind >34 kt) over the western North Pacific in 2004 were investigated using Advanced Microwave Sounding Unit (AMSU) observations in combination with the independently created early stage Dvorak analysis. Typical temperature profiles of the developing and nondeveloping cloud clusters were compared. From this comparison, positive upper-troposphere temperature anomalies were found in both cluster types; however, the spatial extent of the temperature anomalies for the developing cloud clusters was larger than those of the nondeveloping cloud clusters. Statistical analysis was performed on the temperature anomalies near the center of all clusters retrieved from AMSU observational data. Findings indicate that the area-average temperature anomalies increased along with the intensity of the clusters indicated by the Dvorak T-number classification. Using time series .
The maximal number of interior peak solutions concentrating on hyperplanes for a singularly perturbed Neumann problem

We consider the following singularly perturbed elliptic problem $\varepsilon^2 \Delta u-u+f(u)=0, u>0 $ in $B_1$, $\frac{\partial u}{\partial \nu}=0 $ on $\partial B_1,$ where $\Delta = \sum_{i=1}^N \frac{\partial^2}{\partial x_i^2}$ is the Laplace operator, $B_1$ is the unit ball centered at the origin in $R^N$ $(N\ge 3)$, $\nu$ denotes the unit outer normal to $\partial B_1$, $\varepsilon > 0$ is a constant, and $f$ is a superlinear, subcritical nonlinearity . We will show that when $\e$ is sufficiently small there exists a solution with K interior peaks located on a hyperplane, where $1\le K \varepsilon\frac{C}{(\varepsilon)^{N-1}}$ with $C$ a positive constant depending on $N$ and $f$ only. As a consequence, we obtain that there exists at least $[\frac{C}{(\varepsilon)^{N-1}}]$ number of solutions for $\varepsilon$ sufficiently small.
Multimodal motor imagery decoding method based on temporal spatial feature alignment and fusion

Objective. A motor imagery-based brain-computer interface (MI-BCI) translates spontaneous movement intention from the brain to outside devices. Multimodal MI-BCI that uses multiple neural signals contains rich common and complementary information and is promising for enhancing the decoding accuracy of MI-BCI. However, the heterogeneity of different modalities makes the multimodal decoding task difficult. How to effectively utilize multimodal information remains to be further studied. Approach. In this study, a multimodal MI decoding neural network was proposed. Spatial feature alignment losses were designed to enhance the feature representations extracted from the heterogeneous data and guide the fusion of features from different modalities. An attention-based modality fusion module was built to align and fuse the features in the temporal dimension. To evaluate the proposed decoding method, a five-class MI electroencephalography (EEG) and functional near infrared spectroscopy (fNIRS) dataset were constructed. Main results and significance. The comparison experimental results showed that the proposed decoding method achieved higher decoding accuracy than the compared methods on both the self-collected dataset and a public dataset. The ablation results verified the effectiveness of each part of the proposed method. Feature distribution visualization results showed that the proposed losses enhance the feature representation of EEG and fNIRS modalities. The proposed method based on EEG and fNIRS modalities has significant potential for improving decoding performance of MI tasks.
Metabolomics in plant pathogen-defence: from single molecules to large scale analysis.

Plants produce a high diversity of secondary metabolites that are involved in a wide range of different functions, including stress tolerance, signaling molecule for interactions with other species (allelopathy) and protecting plants against herbivores and pathogens. With the rise of more accessible, high-throughput mass spectrometry and new analytical tools it becomes feasible to identify and validate new secondary metabolites involved in pathogen resistance or assign new roles to previously detected compounds. In this review, we provide a brief overview of the major pathogen defence-associated classes of secondary metabolites, with a focus on those with direct anti-pathogen function. For each class we highlight one or more typical examples representing the class to give a comprehensive summary of some of the work done to date. In the second part of this review, we highlight how new technological advances and high throughput experiments in combination with other sources of -omics data, like genomics and transcriptomics can accelerate the studies on secondary metabolites and help to link these compounds to genotypes. Employing such approaches will improve our understanding of chemical defences against plant pathogens and allow rapid development of markers for resistance breeding.
Long noncoding RNAs are substrates for cytoplasmic capping enzyme

Cytoplasmic capping returns a cap to specific mRNAs, thus protecting uncapped RNAs from decay. Prior to the identification of cytoplasmic capping, uncapped mRNAs were thought to be degraded. Here, we test whether long noncoding RNAs (lncRNAs) are substrates of the cytoplasmic capping enzyme (cCE). The subcellular localisation of 14 lncRNAs associated with sarcomas were examined in U2OS osteosarcoma cells. We used 5′ rapid amplification of cDNA ends (RACE) to assay uncapped forms of these lncRNAs. Inhibiting cytoplasmic capping elevated uncapped forms of selected lncRNAs indicating a plausible role of cCE in targeting them. Analysis of published cap analysis of gene expression (CAGE) data shows increased prevalence of certain 5’‐RACE cloned sequences, suggesting that these uncapped lncRNAs are targets of cytoplasmic capping.
Endogenous salicylic acid suppresses de novo root regeneration from leaf explants

Plants can regenerate new organs from damaged or detached tissues. In the process of de novo root regeneration (DNRR), adventitious roots are frequently formed from the wound site on a detached leaf. Salicylic acid (SA) is a key phytohormone regulating plant defenses and stress responses. The role of SA and its acting mechanisms during de novo organogenesis is still unclear. Here, we found that endogenous SA inhibited the adventitious root formation after cutting. Free SA rapidly accumulated at the wound site, which was accompanied by an activation of SA response. SA receptors NPR3 and NPR4, but not NPR1, were required for DNRR. Wounding-elevated SA compromised the expression of AUX1, and subsequent transport of auxin to the wound site. A mutation in AUX1 abolished the enhanced DNRR in low SA mutants. Our work elucidates a role of SA in regulating DNRR and suggests a potential link between biotic stress and tissue regeneration.
210Pb as a Marker of Natural Radioactivity: Would it Play a Role in Some Human Diseases?

Lead-210 is unknown to the general public, but one of its daughters, polonium-210, made newspaper headlines several times in 2006 and 2012. In 2006 the whole world learned that a former Russian KGB agent named Alexander Litvinenko had been poisoned by polonium-210, a powerful short-lived alpha emitter. The ingested poisoning activity was estimated to be more than 1–3 GBq [1], which is ~6 μg of pure 210Po. The experts agree that it is an ideal weapon, impossible to detect with the naked eye and that a lethal dose can be held in a volume the size of a homeopathic pill. In 2012 we learned that traces of 210Po were found on the underwear, hat, and toothbrush of Yasser Arafat, the Palestinian leader who died in 2004. Calculation of the activity at the time of his death shows that poisoning with 210Po is not excluded. The different samples collected during the exhumation will probably answer the question.
Problem gaming-related harm experienced by partners and parents of individuals with gaming problems and their help-seeking experiences

Abstract Background and aims Limited research has investigated how individuals' problem gaming affects significant others. The present study investigated the extent to which partners and parents were personally affected by their partner or child's problematic gaming behavior and what steps, if any, were taken in relation to treatment and other help-seeking by the gamers and the respondents themselves. Methods Two targeted samples (parents, n = 104; partners, n = 264) in Australia were recruited and administered an online survey. The survey assessed gaming-related harm across multiple domains, including financial, relationship, emotional wellbeing, physical health and work/study. Treatment and help-seeking questions referred to seeking psychological assistance, self-help, and community support. Non-parametric tests compared groups on harm measures based on GD status. Results Parents and partners of individuals rated in the ‘problem gaming’ range reported significantly greater harms compared to those in the at-risk and non-problem categories. The most frequently endorsed harms were in the relationship domain, including neglected household responsibilities, withdrawal from social events, and relationship conflict. Some parents consult with friends and family (15%) to resolve their child's gaming-related problems. Partners reported to seek outside support and assistance for themselves, including 30% who sought a psychologist. No partners reported having consulted a psychologist for their gaming partner. Discussion Problem gaming affects significant others across multiple life areas, but few seek outside help or support, suggesting there may be significant unmet needs. Conclusions Further research should examine factors that influence acceptance and engagement with problem gaming help options. Harm indicators may be useful for evaluating targeted interventions and other measures to reduce problem gaming.
Mutual influence of magnetic field decay and thermal evolution of rotational neutron stars

Abstract The effect of magnetic field decay on the chemical heating and thermal evolution of neutron stars is discussed. Our main goal is to study how chemical heating mechanisms and thermal evolution are changed by field decay and how magnetic field decay is modified by the thermal evolution. We show that the effect of chemical heating is suppressed by the star spin-down through decaying magnetic field at a later stage; magnetic field decay is delayed significantly relative to stars cooling without heating mechanisms; compared to typical chemical heating, the decay of the magnetic field can even cause the temperature to turn down at a later stage.
Phylogeography of Aphyocypris normalis Nichols and Pope, 1927 at Hainan Island and adjacent areas based on mitochondrial DNA data

We investigated the genetic structure of the freshwater fish Aphyocypris normalis, in 33 populations around Hainan Island and southern mainland China. Sequencing of the mitochondrial DNA (mtDNA) cytochrome b from 127 specimens yielded 47 haplotypes, from which we inferred a Bayesian tree. This revealed three major divergences: a principal clade of specimens with widespread geographic distribution, plus two clades with limited distribution. We estimated that these diverged between 1.05–0.16 Ma. Additionally, based on molecular data and comparing with the climate patterns of Hainan Island, eight phylogeographic ranges (populations) of A. normalis were constructed: the eastern plain (E), northeastern hills and plain (NE), northwestern hills and lowlands (NW), central mountains (C), southeastern hills and plain (SE), southern mountains and hills (S), southwestern mountains and lowlands (SW), and western lowlands (W). The patterns of geographical divergence in this species do not reflect the isolation caused by the Qiongzhou (Hainan) Strait, which would generally be experienced by terrestrial animals on isolated islands. The present results indicate that the major clades within A. normalis have diverged before the temporary land bridge existed across the strait during the Last Glacial Maximum.
Characterization of eyes, photoreceptors, and opsins in developmental stages of the arrow worm Spadella cephaloptera (Chaetognatha)

Abstract The phylogenetic position of chaetognaths, or arrow worms, has been debated for decades, however recently they have been grouped into the Gnathifera, a sister clade to all other Spiralia. Chaetognath photoreceptor cells are anatomically unique by exhibiting a highly modified cilium and are arranged differently in the eyes of the various species. Studies investigating eye development and underlying gene regulatory networks are so far missing. To gain insights into the development and the molecular toolkit of chaetognath photoreceptors and eyes a new transcriptome of the epibenthic species Spadella cephaloptera was searched for opsins. Our screen revealed two copies of xenopsin and a single copy of peropsin. Gene expression analyses demonstrated that only xenopsin1 is expressed in photoreceptor cells of the developing lateral eyes. Adults likewise exhibit two xenopsin1 + photoreceptor cells in each of their lateral eyes. Beyond that, a single cryptochrome gene was uncovered and found to be expressed in photoreceptor cells of the lateral developing eye. In addition, cryptochrome is also expressed in the cerebral ganglia in a region in which also peropsin expression was observed. This condition is reminiscent of a nonvisual photoreceptive zone in the apical nervous system of the annelid Platynereis dumerilii that performs circadian entrainment and melatonin release. Cryptochrome is also expressed in cells of the corona ciliata, an organ in the posterior dorsal head region, indicating a role in circadian entrainment. Our study highlights the importance of the Gnathifera for unraveling the evolution of photoreceptors and eyes in Spiralia and Bilateria.
Fabrication of solid lipid nanoparticles-based patches of paroxetine and their ex-vivo permeation behaviour

Abstract Paroxetine is not suitable for oral administration due to its extensive first-pass metabolism, thus resulting in less bioavailability. This study aimed to prepare novel paroxetine-loaded solid lipid nanoparticles (SLNs) based sustained-release transdermal patches to overcome these problems by enhancing drug absorption and bioavailability. Nine formulations of paroxetine SLNs were prepared by the hot melt-homogenization method using different concentrations of glycerol monostearate (Kolliwax) and Tween 80. Then these prepared SLNs were incorporated in a matrix type transdermal patch having a matrix of ethyl cellulose and polyvinyl pyrrolidone in 3:2 with polyvinyl alcohol. The SLNs showed a particle size range of 113–230 nm and an entrapment efficiency of 85.14%. The SLNs showed sustained paroxetine release (77.86–95.63% release) up to 48 h. FTIR studies showed no interaction between drug and formulation components. Paroxetine is evenly distributed in an amorphous form in SLNs, as demonstrated by DSC as well as PXRD analysis. SLNs formulated patches showed higher drug permeation through the skin than drug-based transdermal patches. , Draize patch test revealed no sign of erythema after applying paroxetine-loaded SLN patches (score 0) as observed with the marketed product. The developed SLNs based transdermal patches showed increased permeability and sustained release behaviour.
Vorticity and passive-scalar dynamics in two-dimensional turbulence

The dynamics of vorticity in two-dimensional turbulence is studied by means of semi-direct numerical simulations, in parallel with passive-scalar dynamics. It is shown that a passive scalar forced and dissipated in the same conditions as vorticity, has a quite different behaviour. The passive scalar obeys the similarity theory à la Kolmogorov, while the enstrophy spectrum is much steeper, owing to a hierarchy of strong coherent vortices. The condensation of vorticity into such vortices depends critically both on the existence of an energy invariant (intimately related to the feedback of vorticity transport on velocity, absent in passive-scalar dynamics, and neglected in the Kolmogorov theory of the enstrophy inertial range); and on the localness of flow dynamics in physical space (again not considered by the Kolmogorov theory, and not accessible to closure model simulations). When space localness is artificially destroyed, the enstrophy spectrum again obeys a k−1 law like a passive scalar. In the wavenumber range accessible to our experiments, two-dimensional turbulence can be described as a hierarchy of strong coherent vortices superimposed on a weak vorticity continuum which behaves like a passive scalar.
Solid lipid-based nanoparticulate system for sustained release and enhanced in-vitro cytotoxic effect of 5-fluorouracil on skin Melanoma and squamous cell carcinoma

The present study aimed to prepare solid lipid-based nanoparticles (SLNs) using Precirol® ATO 5 as solid lipid and Poloxamer 188 and Tween 80 as surfactant and co-surfactant respectively, and SLNs-derived gel for sustained delivery, enhanced in-vitro cytotoxicity, enhanced cellular uptake of 5-FU and enhanced permeation of 5-FU across the skin. The 5-FU-loaded SLNs were prepared by the hot melt encapsulation method and converted into SLN-derived gel using a gelling agent (Carbopol 940). The 5-FU-loaded SLNs had a particle size in the range of 76.82±1.48 to 327±4.46 nm, zeta potential between -11.3±2.11 and -28.4±2.40 mV, and entrapment efficiency (%) in range of 63.46±1.13 and 76.08±2.42. The FTIR analysis depicted that there was no chemical interaction between 5-FU and formulation components. Differential scanning calorimetric analysis showed thermal stability of 5-FU in the nanoparticles and powdered X-ray diffraction analysis revealed successful incorporation of 5-FU in nanoparticles. The in-vitro release study of 5-FU-loaded SLNs showed biphasic release behavior with initial burst release followed by sustained release over 48 hr. The 5-FU-loaded SLNs showed a greater cytotoxic effect on skin melanoma (B16F10 cells) and squamous cell carcinoma (A-431 cells) as compared to free 5-FU drug solution after 48 hr. Flow cytometry and fluorescence microscopy displayed enhanced quantitative and qualitative cellular uptake of SLNs. The SLNs formulation showed acceptable safety and biocompatible profile after an acute toxicity study in Wistar rats. Moreover, ex-vivo permeation studies depicted 2.13±0.076 folds enhanced flux of 5-FU-loaded SLN derived gel compared to 5-FU plain gel, and skin retention studies revealed target efficiency (%) 2.54±0.03 of 5-FU-loaded SLN derived gel compared to 5-FU plain gel.
Proximity Labeling in Plants

Proteins are workhorses in the cell; they form stable and more often dynamic, transient protein–protein interactions, assemblies, and networks and have an intimate interplay with DNA and RNA. These network interactions underlie fundamental biological processes and play essential roles in cellular function. The proximity-dependent biotinylation labeling approach combined with mass spectrometry (PL-MS) has recently emerged as a powerful technique to dissect the complex cellular network at the molecular level. In PL-MS, by fusing a genetically encoded proximity-labeling (PL) enzyme to a protein or a localization signal peptide, the enzyme is targeted to a protein complex of interest or to an organelle, allowing labeling of proximity proteins within a zoom radius. These biotinylated proteins can then be captured by streptavidin beads and identified and quantified by mass spectrometry. Recently engineered PL enzymes such as TurboID have a much-improved enzymatic activity, enabling spatiotemporal mapping with a dramatically increased signal-to-noise ratio. PL-MS has revolutionized the way we perform proteomics by overcoming several hurdles imposed by traditional technology, such as biochemical fractionation and affinity purification mass spectrometry. In this review, we focus on biotin ligase–based PL-MS applications that have been, or are likely to be, adopted by the plant field. We discuss the experimental designs and review the different choices for engineered biotin ligases, enrichment, and quantification strategies. Lastly, we review the validation and discuss future perspectives.
Induction of membraneless organelles as a strategy for HIV-1 post-nuclear entry steps

As intracellular parasites, viruses must modulate the environment of host cells to facilitate viral genome replication and virion assembly. One strategy is to establish specialized, membrane-associated subcellular structures to concentrate viral and cellular components and mediate viral replication processes. This strategy is utilized by many positive-strand RNA viruses such as coronaviruses, hepatitis C virus, and Zika virus, and they re-organize host endo-membranes to form double-membrane vesicles for viral genome replication (Wolff et al., 2020). A growing number of studies have uncovered another strategy for subcellular organization—generation of membraneless organelles (MLOs), often mediated by liquid–liquid phase separation (LLPS). These MLOs usually contain proteins with multivalent domains or intrinsically disordered regions (IDRs). Weak interactions between multivalent domains and additional interactions between IDRs and nuclear acids are the main driving forces underlying phase separation (Shin and Brangwynne, 2017). For instance, rabies virus, a negative-strand RNA virus, forms MLOs named inclusion