Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Mutations and Non-Coding RNA-Based Epigenetic Alterations Mediating the Warburg Effect in Colorectal Carcinogenesis

Simple Summary Colorectal cancer is one of the most leading causes of death worldwide. The Hallmark of colorectal cancer is the increase of glucose uptake and lactate production even in the presence of oxygen, a phenomenon known as the “Warburg effect”. This review summarizes the genetic mutations and epigenetic alterations, focusing on non-coding RNA associated with the oncogenes, tumor suppresser genes, and enzymes involved in the “Warburg effect”, in addition to their clinical impacts on colorectal cancer. This knowledge may open the door for novel therapeutic approaches to target colorectal cancer. Abstract Colorectal cancer (CRC) development is a gradual process defined by the accumulation of numerous genetic mutations and epigenetic alterations leading to the adenoma-carcinoma sequence. Despite significant advances in the diagnosis and treatment of CRC, it continues to be a leading cause of cancer-related deaths worldwide. Even in the presence of oxygen, CRC cells bypass oxidative phosphorylation to produce metabolites that enable them to proliferate and survive—a phenomenon known as the “Warburg effect”. Understanding the complex glucose metabolism in CRC cells may support the development of new diagnostic and therapeutic approaches. Here we discuss the most recent findings on genetic mutations and epigenetic modulations that may positively or negatively regulate the Warburg effect in CRC cells. We focus on the non-coding RNA (ncRNA)-based epigenetics, and we present a perspective on the therapeutic relevance of critical molecules and ncRNAs mediating the Warburg effect in CRC cells. All the relevant studies were identified and assessed according to the genes and enzymes mediating the Warburg effect. The findings summarized in this review should provide a better understanding of the relevance of genetic mutations and the ncRNA-based epigenetic alterations to CRC pathogenesis to help overcome chemoresistance.
Application of Plant Viruses in Biotechnology, Medicine, and Human Health

Plant-based nanotechnology programs using virus-like particles (VLPs) and virus nanoparticles (VNPs) are emerging platforms that are increasingly used for a variety of applications in biotechnology and medicine. Tobacco mosaic virus (TMV) and potato virus X (PVX), by virtue of having high aspect ratios, make ideal platforms for drug delivery. TMV and PVX both possess rod-shaped structures and single-stranded RNA genomes encapsidated by their respective capsid proteins and have shown great promise as drug delivery systems. Cowpea mosaic virus (CPMV) has an icosahedral structure, and thus brings unique benefits as a nanoparticle. The uses of these three plant viruses as either nanostructures or expression vectors for high value pharmaceutical proteins such as vaccines and antibodies are discussed extensively in the following review. In addition, the potential uses of geminiviruses in medical biotechnology are explored. The uses of these expression vectors in plant biotechnology applications are also discussed. Finally, in this review, we project future prospects for plant viruses in the fields of medicine, human health, prophylaxis, and therapy of human diseases.
An Update on mRNA-Based Viral Vaccines

With the success of COVID-19 vaccines, newly created mRNA vaccines against other infectious diseases are beginning to emerge. Here, we review the structural elements required for designing mRNA vaccine constructs for effective in vitro synthetic transcription reactions. The unprecedently speedy development of mRNA vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was enabled with previous innovations in nucleoside modifications during in vitro transcription and lipid nanoparticle delivery materials of mRNA. Recent updates are briefly described in the status of mRNA vaccines against SARS-CoV-2, influenza virus, and other viral pathogens. Unique features of mRNA vaccine platforms and future perspectives are discussed.
A Comprehensive Review of the Composition, Nutritional Value, and Functional Properties of Camel Milk Fat

Recently, camel milk (CM) has been considered as a health-promoting icon due to its medicinal and nutritional benefits. CM fat globule membrane has numerous health-promoting properties, such as anti-adhesion and anti-bacterial properties, which are suitable for people who are allergic to cow’s milk. CM contains milk fat globules with a small size, which accounts for their rapid digestion. Moreover, it also comprises lower amounts of cholesterol and saturated fatty acids concurrent with higher levels of essential fatty acids than cow milk, with an improved lipid profile manifested by reducing cholesterol levels in the blood. In addition, it is rich in phospholipids, especially plasmalogens and sphingomyelin, suggesting that CM fat may meet the daily nutritional requirements of adults and infants. Thus, CM and its dairy products have become more attractive for consumers. In view of this, we performed a comprehensive review of CM fat’s composition and nutritional properties. The overall goal is to increase knowledge related to CM fat characteristics and modify its unfavorable perception. Future studies are expected to be directed toward a better understanding of CM fat, which appears to be promising in the design and formulation of new products with significant health-promoting benefits.
Availability of an RFID Object-Identification System in IoT Environments

Through the latest technological and conceptual developments, the centralized cloud-computing approach has moved to structures such as edge, fog, and the Internet of Things (IoT), approaching end users. As mobile network operators (MNOs) implement the new 5G standards, enterprise computing function shifts to the edge. In parallel to interconnection topics, there is the issue of global impact over the environment. The idea is to develop IoT devices to eliminate the greenhouse effect of current applications. Radio-frequency identification (RFID) is the technology that has this potential, and it can be used in applications ranging from identifying a person to granting access in a building. Past studies have focused on how to improve RFID communication or to achieve maximal throughput. However, for many applications, system latency and availability are critical aspects. This paper examines, through stochastic Petri nets (SPNs), the availability, dependability, and latency of an object-identification system that uses RFID tags. Through the performed analysis, the optimal balance between latency and throughput was identified. Analyzing multiple communication scenarios revealed the availability of such a system when deployed at the edge layer.
Treatment Guidelines for PTSD: A Systematic Review

Background: The aim of this review was to assess the quality of international treatment guidelines for post-traumatic stress disorder (PTSD), and identify differences between guideline recommendations, with a focus on the treatment of nightmares. Methods: Guidelines were identified through electronic searches of MEDLINE, CINAHL, PubMed, Embase and Science Direct, as well as web-based searches of international guideline repositories, websites of psychiatric organisations and targeted web-searches for guidelines from the three most populous English-speaking countries in each continent. Data in relation to recommendations were extracted and the AGREE II criteria were applied to assess for quality. Results: Fourteen guidelines, published between 2004–2020, were identified for inclusion in this review. Only five were less than 5 years old. Three guidelines scored highly across all AGREE II domains, while others varied between domains. Most guidelines consider both psychological and pharmacological therapies as first-line in PTSD. All but one guideline recommended cognitive behavioural therapy (CBT) as first-line psychological treatment, and selective serotonin reuptake inhibitors (SSRIs) as first-line pharmacological treatment. Most guidelines do not mention the targeted treatment of nightmares as a symptom of PTSD. Prazosin is discussed in several guidelines for the treatment of nightmares, but recommendations vary widely. Most PTSD guidelines were deemed to be of good quality; however, many could be considered out of date. Recommendations for core PTSD symptoms do not differ greatly between guidelines. However, despite the availability of targeted treatments for nightmares, most guidelines do not adequately address this. Conclusions: Guidelines need to be kept current to maintain clinical utility. Improvements are most needed in the AGREE II key domains of ‘applicability’, ‘rigour of development’ and ‘stakeholder involvement’. Due to the treatment-resistant nature of nightmares, guideline development groups should consider producing more detailed recommendations for their targeted treatment. More high-quality trials are also required to provide a solid foundation for making these clinical recommendations for the management of nightmares in PTSD.
Titania-coated gold nanorods as an effective carrier for gambogic acid

Gambogic acid (GA), a natural product, exhibits potent anticancer effects. Unfortunately, further clinical application of this drug is limited by its poor solubility in aqueous solutions. In the present study, titanium dioxide (TiO2)-coated gold nanorod (GNR/TiO2) nanostructures were used as a carrier for GA. The GNR/TiO2 nanostructure-based delivery was found to provide a stable aqueous dispersion of GA. Compared with the free form, enhanced intracellular GA delivery was achieved by using the GNR/TiO2 nanostructures. An in vitro cytotoxicity study indicated that the GA-loaded GNR/TiO2 nanostructures were much more effective in inhibiting the proliferation of human glioblastoma U-87 MG cells than free GA, particularly at lower working concentrations. Furthermore, the GNR/TiO2 nanostructures displayed a high photothermal conversion efficiency, and irradiation at a low dose (5.3 W cm−2, 2 min) with an 808 nm laser also synergistically enhanced the anticancer effect of the GA-loaded GNR/TiO2 nanostructures. Therefore, our results suggest that GNR/TiO2 nanostructure-based drug delivery is a promising method to improve the therapeutic efficacy of GA.
Infant Anthropometry and Growth Velocity Before 6 Months are Associated with Breastfeeding Practices and the Presence of Subclinical Mastitis and Maternal Intestinal Protozoa in Indigenous Communities in Guatemala

ABSTRACT Background The possibility that maternal health status and breastfeeding practices contribute to growth faltering before 6 mo is underexplored. Objectives This longitudinal study investigated whether indicators of subclinical mastitis (SCM) and breast inflammation, maternal fecal-oral contamination, and/or breastfeeding practices were associated with infant anthropometry or growth velocity before 6 mo. Methods Indigenous Mam-Mayan mother-infant dyads (n = 140) were recruited. Breast milk was collected at early (<6 wk) and established (4–6 mo) lactation when maternal and infant anthropometry were measured. Milk Na:K ratio as an indicator of SCM and concentrations of 4 proinflammatory cytokines were measured. Maternal stool samples were examined for the presence of intestinal parasites including nonpathogenic protozoa (Endolimax nana, Iodamoeba bütschlii, Entamoeba coli, Blastocystis hominis). Questionnaires characterized breastfeeding and hygiene practices. Multiple linear regression identified factors associated with infant growth attainment [weight-for-age z-score (WAZ), length-for-age z-score (LAZ), and head circumference-for-age z-score (HCAZ)] and growth velocity (expressed as change per day from early to established lactation). Multiple logistic regression identified factors associated with increased odds of underweight, stunting, and low head circumference at both lactation stages. Results A higher Na:K ratio, individual nonpathogenic protozoa, and specific breastfeeding and hygiene practices were associated with impaired growth before 6 wk and at 4–6 mo in exclusively breastfed infants. Growth velocity for weight was inversely associated with Entamoeba coli but cranial growth was associated positively with Iodamoeba bütschlii whereas feeding colostrum in early lactation was protective and decreased the odds of an HCAZ < −2 SD. Finally, the presence of SCM in early lactation increased the likelihood of both WAZ < −2 SD and LAZ < −2 SD by 6 wk. Conclusions Prevention of SCM can improve early infant weight, but measures that promote the feeding of colostrum and reduce exposure to fecal-oral contamination might be required to minimize infant growth faltering in breastfed infants.
The Impact of Cheering on Sports Performance: Comparison of Serie A Statistics Before and During COVID-19

Background: The role of cheering in home advantage in sports performance is unclear. As anti-coronavirus disease 2019 (COVID-19) restrictive measures have prevented crowds from entering stadiums, analysis of the past two football seasons can reveal important details. Objective: This paper aims to compare the last two football seasons in Italy with the previous six, highlighting changes due to the absence of cheering. Methods: We compared the average percentages of points obtained in home matches from 2013 to 2019 with those in the timelapse 2019-2021. The same operation was performed with referee statistics, such as fouls, penalties, and cards awarded against home teams. To do this, we used Welch's t-test and percentage increases. Pearson and Spearman's correlations were searched between the percentages of points collected in home matches and total points earned from 2013 to 2021. Results: The average percentage of points collected by teams in home matches dropped by 8% (Welch’s t = −4.3). The negative correlations between home collected points and total points in 2013-2019 timelapse have significantly diminished during the last two seasons (Welch’s t = 6.2), approaching zero. Penalties against home teams have increased by 30% (Welch’s t = 2.6), reaching 51.4%. Conclusions: This research provides statistical evidence supporting the crowd’s impact on sports and refereeing performance in Serie A. However, our results also suggest that part of the home advantage is linked to factors independent of the audience. Future research can deepen the above phenomena from a theoretical-psychological point of view.
CNT/g‐C3N4 photocatalysts with enhanced hydrogen evolution ability for water splitting based on a noncovalent interaction

Graphite carbon nitride (g‐C3N4) as a novel photocatalyst has attracted growing attention, but its photocatalytic efficiency should be further improved. Based on the large work function and fast electron conductivity of carbon nanotubes (CNTs), here CNT/g‐C3N4 photocatalysts with improved H2 evolution ability and stable water splitting ability were synthesized. The improvement was attributed to the synergistic effect between CNTs and g‐C3N4. As for the mechanisms, CNTs strongly attracted photoelectrons and, because of excellent conductibility, rapidly transferred photoelectrons from the catalyst interface. Thereby, the photoelectron migration rate and the photogenerated charge separation and the use efficiency of photoelectrons in g‐C3N4 were improved, which largely enhanced the hydrogen production ability. Moreover, the addition of CNTs improved the service life and stability of g‐C3N4‐based photocatalytic H2 production. After 10 hours of visible light irradiation, the maximum H2 yield from the 12‐mg/L CNT/g‐C3N4 (CG12) was 138.7 times larger than that of g‐C3N4 (6548.4 vs 47.2 μmol/g), and the H2 evolution rate was 138.7 times that of g‐C3N4 (654.8 vs 4.72 μmol/g/h). After 50 hours, the apparent quantum efficiency of CG12 was up to 37.9%, indicating that the addition of CNTs improved the photocatalytic splitting and stability of g‐C3N4. The mechanism of photocatalytic hydrogen production and the roles of CNTs in improving water splitting were discussed through characterization and activity experiments. It was found that the addition of CNTs accelerated the migration, separation, and utilization of photoelectrons and thereby significantly enhanced the photocatalytic performance.
The Influence of Biological Factors on Haematological Values in Wild Marsh Harrier (Circus aeruginosus) Nestlings

Simple Summary Haematological examination can be used to address the health state of birds. The present study aimed to investigate how hatching order, brood size, and nest initiation date influence the haemoglobin level, red blood cell count, white blood cell count, and glucose level in the blood of raptor nestlings in which brood reduction occurs during nestling rearing. Blood samples were collected to assess the health status of wild marsh harrier nestlings in the agricultural landscape of eastern Poland. Statistical analyses revealed that hatching order, but not brood size or nest initiation date, had an impact on the haematological data of nestlings. Hatching order affected these data in that haemoglobin levels and red blood cell counts gradually decreased, whereas glucose levels and white blood cell counts increased, from the first- to the last-hatched nestlings. The poor condition of the youngest nestlings reflected in these indices may increase the likelihood of their perishing, and consequently, of brood reduction. Abstract Marsh harrier (Circus aeruginosus) is a species with obligatory cainism, in which hatching asynchrony creates a pronounced size hierarchy in nestlings. The size-related competitive advantage of older nestlings means that they tend to dominate the younger ones, and brood reduction occurs in most nests. The aim of the study was to reference values and carry out a haematological examination in order to evaluate the physiological status and health of nestlings with respect to hatching order, brood size, and nest initiation date. To do so, we examined 19 nests with a total of 58 nestlings from a free-living population of this species located in fishpond complexes in the agricultural landscape of eastern Poland. Repeated blood samples (118 in all) were collected from nestlings. The following parameters were measured using fresh full blood: red blood cell count (RBC), haemoglobin level (Hb), white blood cell count (WBC), and plasma glucose level (Glu). The data were analysed using generalized linear mixed models and linear mixed models ((G)LMM). The study revealed that hatching order, but not brood size or nest initiation date, affected the physical condition of marsh harrier nestlings. Hb levels and RBC counts gradually decreased, whereas Glu levels and WBC counts increased from the first- to the last-hatched nestlings. This result points to the generally poorer condition of the youngest nestlings compared with their older siblings. The poor physiological condition of the youngest nestlings may consequently increase the likelihood of their perishing, and hence, of brood reduction.
Adverse Childhood Experiences Predict Common Neurodevelopmental and Behavioral Health Conditions among U.S. Children

Objective: Adverse childhood experiences (ACEs) can have a significant but variable effect on childhood neurodevelopment. The purpose of this study was to quantify and compare the associations between “household challenge” ACEs and common childhood neurodevelopmental and behavioral health conditions, using nationally representative U.S. data. Method: This study used data from the 2016–2019 National Survey of Children’s Health, a nationwide, population-based, cross-sectional survey. Seven household challenge ACEs (not including child maltreatment) were reported by parents/guardians: parental death, incarceration, divorce/separation, family violence, mental illness, substance abuse, and poverty. Logistic regression with sample weights was used to estimate the odds ratio (OR) for 15 parent-reported neurodevelopmental and behavioral health conditions, by the number of reported ACEs. A dose-response relationship was examined by applying tests of orthogonal polynomial contrasts to fitted logistic regression models. Results: Down syndrome, Tourette syndrome and cerebral palsy were not associated with household challenge ACEs, whereas behavior/conduct problems, depression, and substance abuse were strongly associated, with adjusted ORs ranging from 6.36 (95% confidence interval (CI) 5.53, 7.32) to 9.19 (95% CI 7.79, 10.84). Other neurodevelopmental conditions not traditionally associated with childhood adversity showed moderate yet robust associations with ACEs, including autism (adjusted OR 2.15, 95% CI 1.64, 2.81), learning disability (adjusted OR 3.26, 95% CI 2.80, 3.80), and attention deficit hyperactivity disorder (adjusted OR 3.95, 95% CI 3.44, 4.53). The ORs increased with the number of ACEs, showing significant positive linear trends. Conclusion: We found significant dose-dependent or cumulative associations between ACEs and multiple neurodevelopmental and behavioral conditions.
Circulating miR-122-5p and miR-375 as Potential Biomarkers for Bone Mass Recovery after Parathyroidectomy in Patients with Primary Hyperparathyroidism: A Proof-of-Concept Study

Primary hyperparathyroidism (PHPT) is the leading cause of secondary osteoporosis. Although bone mineral density (BMD) tends to recover after parathyroidectomy in PHPT patients, the degree of recovery varies. Circulating microRNAs (miRNAs) profiles are known to be correlated with osteoporosis and fracture. We aimed to investigate whether osteoporotic fracture-related miRNAs are associated with postoperative BMD recovery in PHPT. Here, 16 previously identified osteoporotic fracture-related miRNAs were selected. We analyzed the association between the preoperative level of each miRNA and total hip (TH) BMD change. All 12 patients (among the 18 patients enrolled) were cured of PHPT after parathyroidectomy as parathyroid hormone (PTH) and calcium levels were restored to the normal range. Preoperative miR-19b-3p, miR-122-5p, and miR-375 showed a negative association with the percent changes in TH BMD from baseline. The association remained robust for miR-122-5p and miR-375 even after adjusting for sex, age, PTH, and procollagen type 1 N-terminal propeptide levels in a multivariable model. In conclusion, preoperative circulating miR-122-5p and miR-375 levels were negatively associated with TH BMD changes after parathyroidectomy in PHPT patients. miRNAs have the potential to serve as predictive biomarkers of treatment response in PHPT patients, which merits further investigation.