Miracle-dz/UniFAR-FTUs huggingface dataset:
Systematic comparison of technical details in CBB methods and development of a sensitive GAP stain for comparative proteomic analysis

Considering the importance of CBB staining in visualizing proteins in 2‐DE gels, any improvement in the existing protocols with high sensitivity and good MS compatibility is of significant importance. In this study, we systematically evaluated the effects of different staining parameters on CBB methods by 1‐DE and 2‐DE, and demonstrated that G‐250 was more suitable for visualizing low‐abundant proteins as well as generating more spots than R‐250, whereas R‐250 had a superior capability for quick staining of high‐abundant proteins. The staining produced by mixing G‐250 and R‐250 in different ratios showed similar sensitivity. Compared with acetic acid, phosphoric acid produced more protein spots. Ammonium‐based stain demonstrated a superior sensitivity than the aluminum‐based one. Based on these findings, a new protocol using CBB G‐250, ammonium sulfate and phosphoric acid (GAP) was developed by incorporating the fixation, sensitization and staining procedures together. The comparison of GAP with other methods revealed that GAP generated more protein spots and had wider applications. The identification of 11 proteins demonstrated that GAP was not only compatible with MS but also obviously reduced in vitro protein modification, and thus could be a preferable protocol in the future proteomic analysis.
Determining the Composition of Proteins in Milk Using a Portable near Infrared Spectrometer

The content ratio of whey proteins to casein can serve as an indicator of milk quality as well as criterion for the presence of milk proteins adulteration, if it exceeds 0.25. The actual task is to produce an effective and portable sensor for the named ratio. The applicability of an inexpensive portable near infrared spectrometer for the specified task was tested in this work. The streams of backscattering and transmission light in the samples of the restored milk were acquired in the form of optical density spectra for a wavelength range of 800–1065 nm using a new two-channel CCD spectrometer. It was calibrated on the content of fat, casein and whey proteins using partial least squares regression. Another objective was to develop a cost-effective way to obtain a set of reference samples with various concentrations of three components that are needed to create the calibration models or to test them. Testing the calibration designed for the measurement of fat, casein and whey proteins in drinking milk showed root mean squared errors of prediction of 0.09% wt, 0.12% wt and 0.06%wt, and correlation coefficients of 0.90, 0.82 and 0.89, respectively. The value of the 95th percentile for the ratio of whey proteins to casein was found to be±0.06, which is sufficiently low for practical use of the method. A comparison of models, one of which was built using the proposed “custom” set of 35 samples and the other which was based on a random set of 81 samples, showed similar acceptable results. Thus, the proposed method is suitable for cost reduction without loss of calibration quality.
Use of low-grade iron ore for producing a nano-adsorbent and a complex fertilizer

We developed a technology that prepares magnetite nanoparticles (MNPs) from a low-grade iron ore and consumes the by-product wastewater in removing phosphorus and nitrogen in water. The silica that inhibits the MNP formation and that is present in the iron ore leachate was removed by co-precipitation with Al(OH)3. From the supernatant, ferric hydroxide was allowed to be precipitated, leaving Mg2+ behind. The precipitated hydroxide was used as a starting material in preparing the MNPs of 5–15 nm in size by co-precipitation. The wastewater containing a large amount of Mg2+ generated from the ferric hydroxide precipitation stage was used to crystallize and ions in the form of struvite. This process enables the reduction of the cost of Mg2+ in water treatment and the cost of iron ore wastewater treatment. The valuable elements contained in the iron ore can be exploited to produce MNPs and struvite powder as a nano-adsorbent and a composite fertilizer, respectively. Our results suggest that low-grade iron ore or tailings can be applied to alleviate water and soil contamination.
Detection of Pathogenic Escherichia coli (E. coli) Using Robust Silver and Gold Nanoparticles

The paper deals with differently synthesized robust metal nanoparticles using chemical synthesis approach for detection of a new pathogenic strain E. coli in water sample. The reactions have been completed using aqueous metal salt solution and trisodium citrate as a capping agent to obtain nanoparticles. The nanoparticles and their interaction against microorganisms have been characterized using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). For different concentration of sodium borohydride, surface plasmon resonance peaks have been obtained at 390 nm to 402 nm for silver nanoparticles and 518 nm to 524 nm for gold nanoparticles. It has been observed to be shifted from 520 nm to 527 nm and 395 nm to 408 nm for gold and silver nanoparticles after the interaction with E. coli respectively. Silver and gold nanoparticles have been synthesized at 80°C exhibited strong surface plasmon resonance peaks respectively at 433 nm and 529 nm. Remarkable shift have been noticed in surface plasma resonance peaks for small amount of E. coli added in the solution.
Characterization of β-cyclodextrin Self-Aggregates for Eugenol Encapsulation

Abstract The application of β-cyclodextrin(CD)-assisted molecular encapsulation of neutraceutical ingredients, functional food in food products, hydrophobic drugs, and volatile substances helps protect the active ingredients against oxidation, loss of volatile compounds and light-induced decomposition and increases the solubility of hydrophobic medicines. Self-assembly aggregation of CDs has been extensively investigated from the aggregation of native CDs to high-order complex aggregates. The increasing complex order of the aggregates is dependent on some parameters such as pH, temperature, time and concentration. The aim of this study was to investigate the effects of the aggregation behaviour of β-cyclodextrin (CD)-eugenol complexes induced at shaking times on the release characteristic at various relative humidity and storage temperatures. The particle size started to increase after shaking for 8 h at 650 nm, and then, significant aggregation (1,110 nm) was observed after 24 h. The retention rate of eugenol significantly increased according to storage duration under various relative humidity and temperature conditions. Prolonged shaking time enhanced the self-aggregation of β-CD-eugenol complexes resulting in eugenol release behavior due to their effect on the aggregation mechanism, as assessed by TEM observation. The aggregation of β-CD-eugenol complexes could be attributed to the slow release of eugenol owing to the presence of a thick layer of β-CD aggregates.
The Theoretical-Conceptual Model of Churning in Human Resources: The Importance of Its Operationalization

Given the current socio-economic context in which the labor market is set in, if we were to consider both employment opportunities in specific economic periods and the individual expectations workers have regarding one’s working conditions, it should be a matter of one’s individual right of choice to decide whether or not to stay or leave and change companies. The paper we present before you took into account the phenomenon of churning as a cyclical process. Our main goal was to understand the main causes leading to it in the context of human resources and, ultimately, what were the consequences emerging from it. In order to carry out this analysis, we put forward a conceptual-theoretical model of the phenomenon of churning, made possible through the analysis of both the currently available literature and the empirical studies and conclusions stemming from the latter. The aforementioned analysis allowed us to trace all main causes responsible for the origin of churning and also, to understand how it could be implemented further on, in order to minimize the costs associated with hiring new personnel and retaining qualified employees by resorting beforehand to the implementation of strategic measures of human resources retention. By applying the methodology based on grounded theory, this study allowed us to further contribute to the already available, yet limited, literature and definition of this multifaceted and greatly complex subject that is the phenomenon of churning.
Crises, desastres naturais e pandemias: contribuições da Psicologia Positiva

In the wake of crises, pandemics, and natural disasters, several devastating consequences concerning the population’s mental health can be observed. For this reason, it is important to understand how psychology can help individuals and communities that experience a traumatic event. This paper will focus on insights from positive psychology in this context, given the topicality of the topic in the face of the global pandemic caused by COVID-19. The paper was organized to point out positive psychology applications, considering three phases: disaster preparedness, crisis response, and recovery phase. The literature review demonstrated that positive psychology could enable individuals to grow, even after experiencing a traumatic event, helping them reformulate their negative experiences, reflect on their priorities, and reassess their values.
The patient experience of bariatric surgery: a longitudinal qualitative study

Morbid obesity is a major public health concern. It is associated with a range of physical and psychological comorbidities including cardiovascular disease, stroke, depression and experiences of psychological distress and stigma. In the UK, treatment for morbid obesity is provided through a tiered pathway of services including bariatric surgery. Outcomes of surgery are not universally positive, with some regaining weight following surgery or experiencing unintended outcomes. This study explored what influences outcomes of bariatric surgery from the patients’ perspective. It aimed to identify how patients measure the success of bariatric surgery, the support needs and mechanisms which influence success and the implications for clinicians, commissioners and policy makers. Using qualitative interviews and modified Photovoice methodology alongside validated quality of life (QoL) measures this longitudinal study explored the expectations and experiences of patients undergoing bariatric surgery. Data was collected in five cycles across the bariatric patients' journey: pre-surgery (n=18); three months post-surgery (n=16); nine months post-surgery (n=15); 18 months post-surgery (QoL measures only) (n=10); and, two years post-surgery (n=13). This is the first study to investigate the experience of more than one service of a tiered obesity pathway. It found that the burdens of living with obesity and expectation of life post-surgery affected the way in which patients determined the success of bariatric surgery. Achieving normality was as important as the standard clinical measures of weight loss and reduced comorbidities. Psychological, physical and social support needs influence the potential for success in these terms. There was clear evidence of temporality, as the measures of success and support needs changed along participants’ bariatric surgery journey. Mechanisms which could address or hinder these support needs included: what participants believed to be a successful outcome; the obesity service pathway; support networks; physical activity and exercise; and excess skin. The findings inform the understanding of what influences the success of bariatric surgery. They are then used to develop practice and policy recommendations including: measures of success should be determined by individual patients and revisited across the journey; health care professionals supporting people living with morbid obesity to lose weight require training to understand the physiological and psychological impacts of bariatric surgery; specialist input from psychological and exercise specialists needs to be available and accessible across the pathway; and clinicians, commissioners and policy makers need to consider the levels of the socio ecological system and respond to the changing support needs in order to maximise chances of long-term successful outcomes for patients.
Las TICs y teorías del aprendizaje: Recursos necesarios para la nueva normalidad educativa

This essay establishes the relationship of the integrating elements in the acquisition of knowledge, establishing the characteristics of the learning theories that interact in the process and the necessary contributions in the formation of total and meaningful learning, taking as a necessary input the new technologies and their application. (prioritizing values in the process), relating their conceptualization and their characteristics according to their application in the pedagogical context. The study focuses its analysis on the structural learning theories that have given way to modalities with similar and / or combined characteristics, allowing the application of teaching-learning strategies and techniques developed through new technologies, for which theories are considered. and streams of learning: conductism, cognitive, constructivist and connectivist. In the same way, the aspects of programs (software) and tools (hardware) that provide mental and psychomotor structures for learning are presented, taking into consideration the continuous production of them and the constant development that society demands in terms of new designs, innovation of others and monitoring of performance related to the teaching-learning process
Clinicopathological Features of Epstein-Barr Virus Infection, Microsatellite Instability, Tumor Mutation Burden and PD-L1 Status in Chinese Patients with Gastric Cancer

Objectives: Gastric cancer (GC) is the 4th most common type of cancer worldwide. Different GC subtypes have unique molecular features that may have different therapeutic methods. The aim of the present study was to investigate Epstein-Barr virus (EBV) infection, microsatellite instability (MSI) status, the expression of programmed death-ligand 1 (PD-L1) and gene mutations in GC patients. Methods: The data of 2,504 GC patients, who underwent curative gastrectomy with lymphadenectomy at Peking University Cancer Hospital between 2013 and 2018, were reviewed. We analyzed the clinicopathological factors associated with the immunohistochemistry (IHC) profiles of these patients, and genetic alterations were analyzed using next generation sequencing (NGS). Results: Mismatch repair-deficient (d-MMR) GC patients were found to have a higher probability of expressing PD-L1 (p<0.001, PD-L1 cutoff value = 1%). In addition, 4 and 6.9% of the 2,504 gastric cancer patients were EBV-positive and d-MMR, respectively. The number of MLH1/PMS2-negative cases was 126 (6%), and the number of MSH2/MSH6-negative cases was 14 (0.9%). d-MMR status was associated with a diffuse/mixed group (p<0.05), but not with tumor differentiation. Furthermore, MSI and d-MMR GC status (detected by NGS and IHC, respectively) were consistently high, and the rate of MSI was higher in patients with d-MMR GC. A number of genes associated with DNA damage repair were detected in GC patients with MSI, including POLE, ETV6, BRCA and RNF43. In patients with a high tumor mutation burden, the most significantly mutated genes were LRP1B (79.07%), ARID1A (74.42%), RNF43 (69.77%), ZFHX3 (65.12%), TP53 (58.14%), GANS (51.16%), BRCA2 (51.16%), PIK3CA (51.16%), NOTCH1 (51.16%), SMARCA4 (48.84%), ATR (46.51%), POLE (41.86%) and ATM (39.53%). Conclusions: Using IHC and NGS, MSI status, protein expression, TMB and genetic alterations were identified in patients with GC, which provides a theoretical basis for the future clinical treatment of GC.
An Add Environmental Dimension Using BIM-LCA integration Technology

In addition to consuming a large amount of energy resources, buildings inevitably destroy the ecological environment in the process of construction. Its main performance in water pollution, air pollution, construction waste and so on. Life Cycle Assessment (LCA) is an international standard method which systematically and quantitatively describes various resources, energy consumption and environmental emissions in product Life Cycle and evaluates their environmental impacts. The integration of building information model and LCA method will avoid repeated modeling and simplify data collection, and provide a certain technical framework for the development of BIM6D. In this paper, the BIM-LCA integration framework and the BIM6D model are established, and the environmental impact of a building in the quantitative analysis is used to guide the development of the BIM6D.
Voltammetric Determination of Ferulic Acid Using a GC Electrode Modified with Poly-aspartic Acid Film

A novel electrochemical sensor was prepared by direct electro-polymerization of DL-aspartic acid on the surface of glassy carbon electrode in aqueous media.In pH 4.5,0.1 mol·L-1 HAc-NaAc buffer solution,the film modified electrode exhibited excellent adsorption capacity to ferulic acid and improved the electrochemical responses significantly.The action mechanism of modified electrode was preliminarily explored,and a fast analytical method for ferulic acid was established.In the range of 9.1×10-7 to 3.0×10-3 mol·L-1,the differential pulse voltammetric peak current had a linear relationship to the concentration,and the detection limit was estimated to be 3.1×10-7mol·L-1.This method was adopted to detect the trace amount of ferulic acid in Chinese patent medicine of Xiaoyao Pills,and the recovery was from 97.9% to 102.2%.
Dialogue, dissémination et matérialisation : une entrevue avec John Durham Peters

John Durham Peters, professor of English and of Film and Media Studies at Yale University, is known for his work on the history of media and communication. His first book, Speaking into the air: A history of the idea of communication, gained worldwide fame thanks to its transdisciplinary outlook on humanity’s thirst for communion, which it finds not in cables and signals, but in its very human condition. In The Marvelous Clouds: Toward a Philosophy of Elemental Media, he invites readers to expand their understanding of media beyond mass media. Francois Cooren, professor at Universite de Montreal’s Department of communication, invited John Durham Peters to give a presentation for the department’s 40th anniversary and took the occasion to discuss with him about his conception of communication. The two men exchange their views, among others, on the need to get past the separation between an apparently immaterial realm of communication and the material world it would merely represent. Benjamin Peters, assistant professor at the University of Tulsa, Oklahoma, joins them and shares his interest in how digital media intersects with regimes of space, time, and power.
Enhanced thermoelectric performance of graphene based nanocomposite coated self-powered wearable e-textiles for energy harvesting from human body heat

The demand for highly flexible and self-powered wearable textile devices has increased in recent years. Graphene coated textile-based wearable devices have been used for energy harvesting and storage due to their outstanding mechanical, electrical and electronic properties. However, the use of metal based nanocomposites is limited in textiles, due to their poor bending, fixation, and binding on textiles. We present here reduced graphene oxide (rGO) as an n-type and conductive polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) as a p-type material for a wearable thermoelectric nanogenerator (TEG) using a (pad–dry–cure) technique. We developed a reduced graphene oxide (rGO) coated textile-based wearable TEG for energy harvesting from low-grade human body heat. The conductive polymer (PEDOT:PSS) and (rGO) nanocomposite were coated using a layer by layer approach. The resultant fabric showed higher weight pickup of 60–80%. The developed textile based TEG device showed an enhanced Seebeck coefficient of (25–150 μV K−1), and a power factor of (2.5–60 μW m−1 K−1). The developed TE device showed a higher potential to convert the low-grade body heat into electrical energy, between the human body temperature of (36.5 °C) and an external environment of (20.0 ± 5 °C) with a temperature difference of (2.5–16.5 °C). The wearable textile-based TEG is capable of producing an open circuit output voltage of 12.5–119.5 mV at an ambient fixed temperature of (20 °C). The rGO coated textile fabric also showed reduced electrical sheet resistance by increasing the number of dyeing cycles (10) and increased with the number of (20) washing cycles. The developed reduced graphene oxide (rGO) coated electrodes showed a sheet resistance of 185–45 kΩ and (15 kΩ) for PEDOT:PSS–rGO nanocomposites respectively. Furthermore, the mechanical performance of the as coated textile fabric was enhanced from (20–80 mPa) with increasing number of padding cycles. The thermoelectric performance was significantly improved, without influencing the breath-ability and comfort properties of the resultant fabric. This study presents a promising approach for the fabrication of PEDOT:PSS/rGO nano-hybrids for textile-based wearable thermoelectric generators (TEGs) for energy harvesting from low-grade body heat.
Percepción de los estudiantes de la Facultad de Economía de la Universidad Autónoma de Yucatán sobre cómo se fomentan o promueven valores en su formación profesional

La finalidad de esta investigación consistió en evaluar el tipo de percepción que poseen los estudiantes de la Facultad de Economía de la Universidad Autónoma de Yucatán (UADY) sobre el modo en que ésta fomenta o promueve valores durante su formación académica-profesional; basado en el cumplimiento del Modelo Educativo para la Formación Integral (MEFI) que rige a la Institución. Se empleó una metodología de tipo investigación acción-participativa, en donde se utilizó como instrumento una guía de preguntas previamente elaboradas que fue tratada entre los grupos participantes por medio de un foro participativo o grupo de enfoque, donde el criterio de inclusión de la muestra fue que los estudiantes pertenecieran a la Facultad de Economía, ya sea en calidad de ingreso, tránsito o egreso y, en cualquiera de los dos programas educativos ofertados por la dependencia; tampoco importó el género de los participantes. La percepción general del estudiantado de la Facultad de Economía de la UADY en cuanto a la formación de valores consiste en que éstos se encuentran en consolidación y no se encuentran total mente presentes dentro de los procesos de enseñanza-aprendizaje involucrados dentro de las actividades académicas.