Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficacy of Infliximab in Disease Control of Refractory Orbital Myositis

ABSTRACT Purpose: Orbital myositis is a common cause of orbital inflammation with localized involvement of the extra ocular muscles. This study aimed to assess the value of infliximab in controlling orbital myositis and reducing its relapse rate. Methods: We conducted a retrospective review of the medical records of all consecutive patients with orbital myositis treated with infliximab between 2007 and 2016. We examined change in corticosteroid and immunomodulatory doses as well as relapse rates following treatment with infliximab. Results: The study included seven patients with an average follow up of 19 ± 13.4 months. The mean dose of systemic corticosteroid was reduced from 28.57 ± 14.35 mg/day at the time of infliximab initiation to 7.00 ± 6.83 mg/day at final follow-up (p = .003). Long-term remission was achieved in 85.7% (n = 6). Conclusion: This study supports the role of infliximab in treating refractory orbital myositis and this was associated with clinical improvement, decreasing relapse rate with dose reduction of conventional treatment.
Chromosome clustering in mitosis by the nuclear protein Ki-67

Ki-67 is highly expressed in proliferating cells, a characteristic that made the protein a very important proliferation marker widely used in the clinic. However, the molecular functions and properties of Ki-67 remained quite obscure for a long time. Only recently important discoveries have shed some light on its function and shown that Ki-67 has a major role in the formation of mitotic chromosome periphery compartment, it is associated with protein phosphatase one (PP1) and regulates chromatin function in interphase and mitosis. In this review, we discuss the role of Ki-67 during cell division. Specifically, we focus on the importance of Ki-67 in chromosome individualisation at mitotic entry (prometaphase) and its contribution to chromosome clustering and nuclear remodelling during mitotic exit.
External gap progression after cyclic fatigue of adhesive overlays and crowns made with high translucency zirconia or lithium silicate

Abstract Objectives To evaluate three‐dimensional external gap progression after chewing simulation of high translucency zirconia (HTZ) and zirconia‐reinforced lithium silicate (ZLS) applied on endodontically treated teeth with different preparation designs. Materials and Method Endodontically treated molars were prepared with low‐retentive (adhesive overlay) and high‐retentive (full crown) designs above cementum‐enamel junction and restored with HTZ and ZLS. Micro‐computed tomography analysis was assessed before and after chewing simulation to evaluate three‐dimensionally the external gap progression. Results were statistically analyzed with two‐way ANOVA and post‐hoc Tukey test. Results High‐retentive preparation design had a significantly inferior gap progression compared to the overlay preparation (p < 0.01); ZLS exhibited a significant inferior gap progression compared to HTZ (p < 0.01). Conclusions High‐retentive preparations restored with ZLS seem to better perform in maintaining the sealing of the external margin after cyclic fatigue. Clinical significance The clinician should pay attention to the proper combination of preparation designs and ceramic material selection for an endodontically treated molar restoration. HTZ seems to perform worse than lithium silicate in terms of marginal sealing, still showing lacks in resistance to cyclic fatigue when adhesive preparations are performed.
Ancestral Flowering Plant Chromosomes and Gene Orders Based on Generalized Adjacencies and Chromosomal Gene Co-Occurrences

Recurrent whole genome duplication and the ensuing loss of redundant genes-fractionation-complicate efforts to reconstruct the gene orders and chromosomes of the ancestors associated with the nodes of a phylogeny. Loss of genes disrupts the gene adjacencies key to current techniques. With our RACCROCHE pipeline, instead of starting with the inference of short ancestral segments, we suggest delaying the choice of gene adjacencies while we accumulate many more syntenically validated generalized (gapped) adjacencies. We obtain longer ancestral contigs using maximum weight matching (MWM). Similarly, we do not construct chromosomes by successively piecing together contigs into larger segments, but rather compile counts of pairwise contig co-occurrences on the set of extant genomes and use these to cluster the contigs. Chromosome-level contig assemblies for a monoploid genome emerge naturally at each node of the phylogeny and the contigs then can be ordered along the chromosome. Sampling alternative MWM solutions, visualizing heat maps, and applying gap statistics allow us to estimate the number of chromosomes in the reconstruction. We introduce several measures of quality: length of contigs, continuity of contig structure on successive ancestors, coverage of the extant genome by the reconstruction, and rearrangement relations among the inferred chromosomes. The reconstructed ancestors are visualized by painting the ancestral projections on the descendant genomes. We submit genomes drawn from a broad range of monocot orders to our pipeline, confirming the tetraploidization event "tau" in the stem lineage between the alismatids and the lilioids. We show additional applications to the Solanaceae and to four Brassica genomes, producing evidence about the monoploid ancestor in each case.
Witnessing Intimate Partner Violence as a Child and Associated Consequences

Intimate partner violence (IPV) continues to be a global problem. The increasing international research, focussing on children who witness IPV between their parents, indicates that these circumstances might influence children in different ways. Despite these efforts, little evidence stems from Germany. Using data from a German school student survey from 2017 and 2019 (N = 21,382), this paper aims to examine the consequences of witnessing IPV. The present paper takes a closer look at children’s violent behaviour, attitudes and quality of life while controlling for other potential influences (e.g. physical and psychological child abuse). Results indicate that IPV has a significant negative effect on children’s quality of life, in particular on children’s sense of security at home and children’s life satisfaction. No other effects were significant. In discussing these complex dynamics, the results of the present study highlight the importance of considering children who witness IPV as direct victims. A home with personal threats cannot provide emotional and physical security. Coping with this absence of a secure base at home may lead to the neglect of important developmental tasks during that time, which can have possible far-reaching implications on different areas of children’s lives (e.g. social competence). Further research examining the severity and duration of witnessing IPV within the context of a longitudinal design is recommended.
Neurodynamics: is tension contentious?

ABSTRACT Tensioning techniqueswere the first neurodynamic techniques used therapeutically in the management of people with neuropathies. This article aims to provide a balanced evidence-informed view on the effects of optimal tensile loading on peripheral nerves and the use of tensioning techniques. Whilst the early use of neurodynamics was centered within a mechanical paradigm, research into the working mechanisms of tensioning techniques revealed neuroimmune, neurophysiological, and neurochemical effects. In-vitro and ex-vivo research confirms that tensile loading is required for mechanical adaptation of healthy and healing neurons and nerves. Moreover, elimination of tensile load can have detrimental effects on the nervous system. Beneficial effects of tensile loading and tensioning techniques, contributing to restored homeostasis at the entrapment site, dorsal root ganglia and spinal cord, include neuronal cell differentiation, neurite outgrowth and orientation, increased endogenous opioid receptors, reduced fibrosis and intraneural scar formation, improved nerve regeneration and remyelination, increased muscle power and locomotion, less mechanical and thermal hyperalgesia and allodynia, and improved conditioned pain modulation. However, animal and cellular models also show that ‘excessive’ tensile forces have negative effects on the nervous system. Although robust and designed to withstand mechanical load, the nervous system is equally a delicate system. Mechanical loads that can be easily handled by a healthy nervous system, may be sufficient to aggravate clinical symptoms in patients. This paper aims to contribute to a more balanced view regarding the use of neurodynamics and more specifically tensioning techniques.
The Spatial Logic of Parks

Urban parks are usually studied as discrete, green public open spaces. Less studied is how parks are geographically distributed from a ‘spatial logic’ point of view, i.e. how they ought to be geographically distributed across the urban landscape. This paper evaluates the degree to which normative principles about park distribution are in evidence from the standpoint of three spatial goals: proximity, diversity and social need. Using Phoenix and Chicago as case studies, it offers an empirical example of how park distribution can be shown to conform (or not) to one or more distributive patterns. Descriptive measures show that Phoenix parks do not conform to any of these three distributional principles, while Chicago parks do somewhat better. In both cities, land uses surrounding parks heavily favour residential uses. In Phoenix, single-family homes are prioritized, making spatial distribution based on proximity, diversity or social need difficult. A strategy for acquiring a better spatial logic, within existing realities and constraints, is suggested.
All roads lead to Rome — a review of the potential mechanisms by which exerkines exhibit neuroprotective effects in Alzheimer's disease

Age-related neurodegenerative disorders such as Alzheimer’s disease (AD) have become a critical public health issue due to the significantly extended human lifespan, leading to considerable economic and social burdens. Traditional therapies for AD such as medicine and surgery remain ineffective, impractical, and expensive. Many studies have shown that a variety of bioactive substances released by physical exercise (called “exerkines”) help to maintain and improve the normal functions of the brain in terms of cognition, emotion, and psychomotor coordination. Increasing evidence suggests that exerkines may exert beneficial effects in AD as well. This review summarizes the neuroprotective effects of exerkines in AD, focusing on the underlying molecular mechanism and the dynamic expression of exerkines after physical exercise. The findings described in this review will help direct research into novel targets for the treatment of AD and develop customized exercise therapy for individuals of different ages, genders, and health conditions.
Plant height heterosis is quantitatively associated with expression levels of plastid ribosomal proteins

Significance Heterosis (hybrid vigor) boosts the productivity and resilience of crops and livestock above the levels of both parents, yet its underlying mechanisms remain unknown. We analyzed expression patterns of proteins in maize hybrids and their inbred parents. Differences in several molecular machines and biochemical pathways were found and quantitatively assessed using a panel of 15 hybrids. Seedling leaf chloroplast ribosomal proteins were able to quantitatively infer levels of adult plant heterosis. Expression levels of biosynthetic enzymes for the stress hormone ethylene were reduced in hybrids, as was previously reported for the dicot Arabidopsis. Mutation of these genes in a maize inbred caused the proteome to resemble a hybrid. Repression of ethylene biosynthesis may be a conserved component of heterosis physiology. The use of hybrids is widespread in agriculture, yet the molecular basis for hybrid vigor (heterosis) remains obscure. To identify molecular components that may contribute to trait heterosis, we analyzed paired proteomic and transcriptomic data from seedling leaf and mature leaf blade tissues of maize hybrids and their inbred parents. Nuclear- and plastid-encoded subunits of complexes required for protein synthesis in the chloroplast and for the light reactions of photosynthesis were expressed above midparent and high-parent levels, respectively. Consistent with previous reports in Arabidopsis, ethylene biosynthetic enzymes were expressed below midparent levels in the hybrids, suggesting a conserved mechanism for heterosis between monocots and dicots. The ethylene biosynthesis mutant, acs2/acs6, largely phenocopied the hybrid proteome, indicating that a reduction in ethylene biosynthesis may mediate the differences between inbreds and their hybrids. To rank the relevance of expression differences to trait heterosis, we compared seedling leaf protein levels to the adult plant height of 15 hybrids. Hybrid/midparent expression ratios were most positively correlated with hybrid/midparent plant height ratios for the chloroplast ribosomal proteins. Our results show that increased expression of chloroplast ribosomal proteins in hybrid seedling leaves is mediated by reduced expression of ethylene biosynthetic enzymes and that the degree of their overexpression in seedlings can quantitatively predict adult trait heterosis.
Downregulation of microRNA-512-3p enhances the viability and suppresses the apoptosis of vascular endothelial cells, alleviates autophagy and endoplasmic reticulum stress as well as represses atherosclerotic lesions in atherosclerosis by adjusting spliced/unspliced ratio of X-box binding protein 1 (

ABSTRACT AS is an important pathological basis of cardiovascular disease. miRNAs are involved in almost all steps of AS, including the injury and dysfunction of endothelial cells and vascular smooth muscle cells. This work elucidated the biological functions of miR-512-3p in AS and probed into the underlying molecular mechanism. In the present work, ox-LDL-treated HUVECs served as the in vitro model of AS and ApoE-/- mice were nourished with a high-fat diet to establish an in vivo model of AS. Proliferation, apoptosis, and migration of HUVECs were evaluated by CCK-8, TUNEL staining, Western blot, and transwell assays. Immunofluorescence examined LC3 expression and levels of autophagy-related and ER stress-related proteins were determined by Western blot assay. In addition, starBase predicted the complementary binding sites of XBP-1 to miR-512-3p and luciferase reporter assay confirmed the interaction between miR-512-3p and XBP-1. Moreover, H&E staining was employed to evaluate atherosclerotic lesions in AS model mice. Results revealed that ox-LDL treatment decreased the proliferative and migrative activities and promoted the apoptosis of HUVECs as well as induced autophagy and ER stress, which were abrogated by miR-512-3p silencing. Importantly, ox-LDL treatment elevated miR-512-3p expression and XBP-1 was a direct target of miR-512-3p. Mechanistically, knockdown of miR-512-3p enhanced the viability, suppressed the apoptosis, and promoted the migration of ox-LDL-treated HUVECs, alleviated atherosclerotic lesions in AS model mice as well as repressed autophagy and ER stress by targeting XBP-1 to manipulate the ratio of XBP-1S/XBP-1 U.
General precedes specific in memory representations for structured experience.

Decades of work has shown that learners rapidly extract structure from their environment, later leveraging their knowledge of what is more versus less consistent with prior experience to guide behavior. However, open questions remain about exactly what is remembered after exposure to structure. Memory for specific associations-transitions that unfold over time-is considered a prime candidate for guiding behavior. However, other factors could influence behavior, such as memory for general features like reliable groupings or within-group positions. We also do not yet know whether memory depends upon the amount of experience with the input structure, leaving us with an incomplete understanding of how statistical learning supports behavior. In 4 experiments, we tracked the emergence of memory for item-item transitions, order-independent groups, and positions by having 400 adults watch a stream of shape triplets followed by a recognition memory test. We manipulated how closely test sequences corresponded to the input along each dimension of interest, allowing us to isolate the contribution of each factor. Both item-item transitions and order-independent group information influenced behavior, highlighting statistical learning as a mechanism through which we form both specific and generalized representations. Moreover, these factors drove behavior after different amounts of experience: With limited exposure, only group information impacted old-new judgments specific transitions gained importance later. Our findings suggest statistical learning proceeds by first forming a general representation of structure, with memory being later refined to include specifics after more experience. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
Mindfulness for Outpatient Oncology Nurses

This prospective pre-/posttest pilot studied a mindfulness-based stress reduction (MBSR) intervention first, to reduce self-reported stress and improve compassion satisfaction; and second, to reduce physiological indices of stress. Even small interventions of MBSR before a shift effectively reduces the physiological indices of stress and may help reduce compassion fatigue and burnout in nurses.
High-Temperature Operation of Normally Off-Mode AlGaN/GaN Heterostructure Field-Effect Transistors with p-GaN Gate

We demonstrated the high-temperature operation of normally off-mode heterostructure field-effect transistors (HFETs) with a p-GaN gate. The HFETs with a p-GaN gate were operated in the normally off mode at 350 °C. The temperature dependence of their performance was compared with the results of simulation.
CMOS Cantilever Sensor Systems: Atomic Force Microscopy and Gas Sensing Applications

1. Introduction. - 2. Design Considerations. - 3. Cantilever Beam Resonators. - 4. Resonant Gas Sensor. - 5. Force Sensors for Parallel Scanning Atomic Force Microscopy. - 6. Conclusions and Outlook. - Appendices. - A.1 Process Sequence Resonant Gas Sensor. - A.2 Process Sequence Resonant Gas Sensor (Maskless). - A.3 Process Sequence AFM Sensor Arrays. - A.4 Material Properties of Thin Film Materials. - References.
Regulation of transforming growth factor-beta1 by circANKS1B/miR-515-5p affects the metastatic potential and cisplatin resistance in oral squamous cell carcinoma

ABSTRACT Oral squamous cell carcinoma (OSCC) is the most common oral cancer, with an increasing worldwide incidence and a worsening prognosis. Emerging evidence confirms that circular RNAs (circRNAs) play a critical role in tumor progression via sponging miRNAs. A previous study substantiated the function of circANKS1B in several cancers. However, its role in OSCC remains unclear. This study revealed the high expression of circANKS1B in OSCC tissues and cells. Moreover, the expression level of circANKS1B was highly positively correlated with the expression of transforming growth factor-beta1 (TGF-β1) in OSCC tissues. Additionally, overexpression of circANKS1B enhanced the protein expression of TGF-β1 in OSCC cells, while its inhibition reduced TGF-β1 protein levels. Noticeably, the loss-function of circANKS1B restrained OSCC cell invasion, migration, and epithelial to mesenchymal transition (EMT) by decreasing N-cadherin expression and enhancing E-cadherin expression. Furthermore, the knockdown of circANKS1B sensitized OSCC cells to cisplatin by suppressing cell viability and increasing cell apoptosis and caspase-3 activity. Mechanically, bioinformation software (circinteractome and starBase 3.0) and dual-luciferase reporter assays corroborated that circANKS1B could sponge miR-515-5p. Moreover, miR-515-5p could directly target TGF-β1 to suppress its expression. Importantly, inhibition of miR-515-5p or supplementation with TGF-β1 overturned the effects of circANKS1B knockdown on cell invasion, migration, and cisplatin resistance. Thus, these findings highlight that circANKS1B might act as an oncogenic gene to facilitate the metastatic potential and cisplatin resistance in OSCC by sponging miR-515-5p to regulate TGF-β1. Collectively, circANKS1B may be a promising target for therapy and overcoming chemoresistance in OSCC.
Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9.

Food allergies are a major health issue worldwide. Modern breeding techniques such as genome editing via CRISPR/Cas9 have the potential to mitigate this by targeting allergens in plants. This study addressed the major allergen Bra j I, a seed storage protein of the 2S albumin class, in the allotetraploid brown mustard (Brassica juncea). Cotyledon explants of an Indian gene bank accession (CR2664) and the German variety Terratop were transformed using Agrobacterium tumefaciens harboring binary vectors with multiple sgRNAs to induce either large deletions or frameshift mutations in both Bra j I homoeologs. A total of 49 T0 lines was obtained with up to 3.8 % transformation efficiency. Four lines had large deletions of 566 up to 790 bp in the Bra j IB allele. Among 18 Terratop T0 lines, nine carried indels in the targeted regions. From 16 analyzed CR2664 T0 lines, 14 held indels and three had all four Bra j I alleles mutated. The majority of the CRISPR/Cas9-induced mutations were heritable to T1 progenies. In some edited lines, seed formation and viability were reduced and seeds showed a precocious development of the embryo leading to a rupture of the testa already in the siliques. Immunoblotting using newly developed Bra j I specific antibodies revealed the amount of Bra j I protein to be reduced or absent in seed extracts of selected lines. Removing an allergenic determinant from mustard is an important first step towards the development of safer food crops.
Modal Placement Control Method for Fractional Systems: Application to a Testing Bench

This paper is dedicated to modal placement control for fractional systems. The influence of the complex plane eigenvalue locations on their time response is discussed for two elementary fractional systems. A dynamic output feedback modal placement control method is proposed. It is applied to the temperature control of a thermal system whose dynamic model is obtained through system identification. © 2003 ASME