JOURNAL OF MODERN DRUG DISCOVERY AND DRUG DELIVERY RESEARCH

Traditional medicine has played a main role in India. Anti Quorum sensing activity was detected on Nyctanthes arbor-Tristis [leaf,flower and stem]extracts and confirmed on by using Pyocyanin quantification assay, Staphylolytic broth activity and Biofilm inhibition assays. The moderate amount of proteins were detected in SDS-PAGE analysis and was observed using silver staining. The FTIR, HPLC analysis showed the presence of compounds like alkaloids, phenols. terpenoids, saponins. flavonoids and glycosides. It also includes Benzoic acid, octadecane, cyclopentane, oleic acid, Tetra decane, pentacosane etc.The GC-MS analysis revealed the presence of 63 natural compounds from the Nyctanthes arbor-Tristis [leaf,stem,and flower] extracts. Some of the active compounds include Heneicosane, dodecanoic acid, eicosane Oleic Acid, tetracontane, Cyclopenta, tetradecanoic acid, eicosane, dodecanoic acid etc. The bioactive constituents found in the ethanol extracts of Nyctanthes arbor-Tristis [leaf, stem, and flower] could act as the lead compounds, or pharmacological agents. These bioactive metabolites have the possibility to act as molecular targets in the drug development. The prime initiative should focus on the cultivation and conservation of medicinal plants with pharmacological importance. Keyword: Anti Quorum, Nyctanthes arbor-Tristis, HPLC,FTIR,GCMS. INTRODUCTION Nyctanthes arbor-tristis Linn. (Oleaceae) is a traditional Indian medicinal plant, commonly called as Night Jasmine (Pavalamalli in Tamil) is a small tree growing up to 10 m tall, with flaky grey bark which is widely cultivated throughout India as a garden plant and found wild in the forests of South Indian regions. It is a traditional plant used in the treatment of chronic fevers and rheumatism [1]. The extracted leaf juice along with the sugar acts as a cholagogue, laxative and mild bitter tonic hence given to children as a remedy for intestinal ailments. Potential anti-malarial activity (against malarial fever has also been found associated with Nyctanthes arbortrisits Leaves [2]. The medicinal properties of the Nyctanthes arbor-tristis is extensively used in Ayurveda, Siddha and Unani systems of medicines. Different parts of Nyctanthes arbor-tristis are known to possess medicinal properties for various ailments by tribal people in India. Juice of the leaves is used as digestive, antidote to reptile venoms, laxative, diaphoretic and dieuretic [3]. β-Sitosterol isolated from N. arbortristis leaves showed analgesic and anti-inflammatory activity Iridoid glucoside isolated from this plant has antileishmanial activity. Ethanolic flower extract of this plant is used for the synthesis of gold nanoparticles.Arbortristoside-A isolated from seeds possesses anti-inflammatory and analgesic activity. Leaf and fruitextracts are useful in the treatment of arthritis. Arbortristoside A and arbortristoside C isolated from plant showed antiviral activity [4]. Ethanol extract of the leaves of Nyctanthes arbor-tristis inhibited acute inflammatory oedema produced by carragenan, formalin, histamine, 5hydroxytryptamine and hyalouronidase in hind paw of rats. It also reduced acute inflammatory swelling in the knee joint induced by turpentine oil [5]. Methanol extract of leaves of Nyctanthes arbortristis exhibited significant hepatoregenerative potential in acetaminophen-induced hepatic damage. It acts by protecting against membrane fragility and by preventing decline in glutathione levels [6]. The aqueous extracts of the Nyctanthes arbor-tristis leaves and seeds showed antihepatotoxic activity against CCl4 induced hepatotoxicity. Similarly ethanol extracts of Nyctanthes arbor-tristis seeds, leaves and flowers caused decreased in the dopamine and increased in serotonin levels. This showed the effect on central nervous system [7]. Aqueous leaf extract of Nyctanthes arbor tristis act as a potent immunomodulator of both humoral and cell mediated responses. Seed of Nyctanthes arbortristis contains 15% of pale yellow brown oil, nyctanthic acid, nyctoside A, b-sitosterol, arbortristoside A & B, glycerides of linoleic oleic, lignoceric, stearic, palmitic and myristic acids, 3-4 secotriterpene acid and a polysaccharide. It is also composed of Dglucose and D mannose which is used as immunostimulant and hepatoprotective [8]. The presence of bioactive metabolites such as polysaccharides, iridoid glycosides, phenypropanoid glycoside, ß-sitosterol, ß-amyrin, hentri-acontane, benzoic acid, glycosides, nyctanthoside -a iridoid, nyctanthic acid, Friedelin lupeol oleanolic acid and 6ßhydroxylonganin, iridoid glucosidesarborsides A, B C, alkaloids, Phlobatanins, terpenoids and cardiac glycosides from the leaf extract of Nyctanthes arbortristis [9]. N. arbor-tristis flowers yielded iridoid glucosides exhibited antiplasmodial activity against Plasmodium falciparum . Besides these compounds, 4hydroxyhexahydro-benzofuran-7-one nyctoside A, arborside C, arborside D, 6-hydroxyloganin, arbortristoside A, arbortristoside B and nyctanthoside have been reported [10]. The plants used, as drugs are fairly innocuous and relatively free from toxic effects or were so toxic that lethal effects were well known. The nature has provided the storehouse of remedies to cure all ailments of mankind. There is no doubt that plants are a reservoir of potentially useful chemical compounds which serve as drugs, are provided newer leads and clues for modern drug design by