Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptome analysis of transgenic apple fruit overexpressing microRNA172 reveals candidate transcription factors regulating apple fruit development at early stages

Background MicroRNA172 (miR172) has been proven to be critical for fruit growth, since elevated miR172 activity blocks the growth of apple (Malus x domestica Borkh.) fruit. However, it is not clear how overexpression of miR172 affects apple fruit developmental processes. Methods To answer this question, the present study, analyzed global transcriptional changes in miR172-overexpressing (miR172OX) and nongenetically modified wild-type (WT) apple fruit at two developmental stages and in different fruit tissues via RNA-seq. In addition, two cultivars, ‘Hanfu’ and ‘M9’, which have naturally fruit size variation, were included to identify miR172-dependent DEGs. qRT–PCRwas used to verify the reliability of our RNA-seq data. Results Overexpression of miR172 altered the expression levels of many cell proliferation- and cell expansion-related genes. Twenty-four libraries were generated, and 10,338 differentially expressed genes (DEGs) were detected between miR172OX and WT fruit tissues. ‘Hanfu’ and ‘M9’ are two common cultivars that bear fruit of different sizes (250 g and 75 g, respectively). Six libraries were generated, and 3,627 DEGs were detected between ‘Hanfu’ and ‘M9’. After merging the two datasets, 6,888 candidate miR172-specific DEGs were identified. The potential networks associated with fruit size triggered traits were defined among genes belonging to the families of hormone synthesis, signaling pathways, and transcription factors. Our comparative transcriptome analysis provides insights into transcriptome responses to miR172 overexpression in apple fruit and a valuable database for future studies to validate functional genes and elucidate the fruit developmental mechanisms in apple.
Thermal, mechanical investigation and neutron shielding analysis for Gd-MOF/polyimide materials

None of the currently commercialized shielding materials in Generation IV nuclear energy systems are satisfactory in their performance. Developing a candidate neutron shielding material with good heat resistance and high strength is a challenging task. In this work, various gadolinium metal–organic frameworks (Gd-MOFs) with obvious advantages, such as porous structures, organic surfaces and strong neutron-absorbing nuclei, were synthesized to constrain polyimide (PI) chains. A series of Gd-MOF/PI conjugates were subsequently assessed for their thermal stability, mechanical properties and neutron shielding performance. The increase of the Gd-MOF content improved the thermal neutron shielding ability but slightly reduced the fast neutron shielding ability. Compared with those of pure PI, the Gd-MOF/PI films demonstrate a higher glass transition temperature (Tg), which is considered the gold standard of engineering plastics. It was also observed that the tensile strength directly correlates with the Gd-MOF content, which continuously increases until a maximum is reached, and then subsequently decreases. Furthermore, the high-temperature tensile test showed that these tunable Gd-MOF/PI films are intact and robust, indicating their potential application for neutron shielding materials in Generation IV nuclear energy systems.
On digital single-sideband modulators

This paper concerns digital single-sideband modulators with analog inputs and outputs. A group of 12 modulators is treated as a single system, with analog-to-digital converters at 12 input ports and a digital-to-analog converter at a single output port. Tradeoffs are obtained between computational parameters. Substitution of digital counterparts for the phase shifters and product modulators in 12 analog Hartley modulators gives a simple program. However, operation in real time requires quite a large number of multiplications per second. A similar digitalization of 12 Weaver modulators requires even more multiplications. However, the 12 Weaver modulators can be transformed as a system, to reduce the multiplication rate for the Hartley system by a factor 4 . The cost is an increase in scratch pad storage and a more complex program.
Broadband high-impedance surface design for aircraft HIRF protection

The design is presented for a broadband high-impedance surface (HIS) and this technology is applied in the field of aircraft EMC. The HIS is analysed by means of a lumped-element equivalent circuit model and a parametric study is undertaken to understand the effect of each parameter on the resonant frequency and bandwidth of the structure. The design of a structure with a bandgap of 2–6 GHz (100% bandwidth) is detailed. TLM simulation and measurement validation of the structure's reflection phase and surface current suppression characteristics show good agreement with each other. An application for the proposed structure, employed on the nacelle of an aircraft, protecting against the penetration of surface currents into sensitive areas, is also discussed.
Effect of fiber nonlinearity on long-distance transmission

The effect of the weak refractive-index nonlinearity of optical fibers on pulse shape is investigated using computer simulations of long-distance transmission. Fiber losses are canceled by periodically spaced optical amplifiers whose spontaneous emission noise is, however, not included in the simulations. The analysis is confined to normal pulses and does not consider solitons. Several conclusions are drawn. (1) If wavelength division multiplexing (WDM) of two channels is used in a uniform fiber without dispersion fluctuations, catastrophic buildup of four-wave mixing occurs if one primary channel is located at the zero-dispersion wavelength. (2) If two pulses with different carrier frequencies collide in a uniform fiber with no gain or loss discontinuities, their four-wave mixing products reach a peak during complete pulse overlap, but this spurious power dies away as the pulses separate. (3) Two-channel WDM transmission of light modulated in amplitude-shift keying format appears feasible at 2.5 GB/s over distances of 7500 km. >
Changing the Health Behaviour of Students During the COVID-19 Pandemic in Poland

The COVID-19 pandemic has caused unprecedented changes in educational, professional, and leisure activities around the world. Protecting health against the virus has become the most important task in the social and individual dimension. The aim of the study was to diagnose changes in the behaviour and health beliefs of students. The WHO definition of health and the Health Belief Model (HBM) were adopted as the theoretical basis for defining variables. The study was of diagnostic and verification nature, and a quantitative strategy was used in it. The general population comprised students of Nicolaus Copernicus University in Torun. The study was conducted electronically in 2020, during the first lockdown in Poland. The Likert scale was used as a tool to assess the degree of change. The respondents declared the greatest changes in terms of caring about physical and relational health. The declared behavioural changes concerned the most conservative, trained forms of pro-health activity. Despite the declared beliefs about the importance of maintaining health and the personal risk of viral infection, the respondents declared undertaking more advanced pro-health activities only to a small extent.
Well-Being Programme for Caregivers of Persons with Spinal Cord Injury

Background: SCI is a high-cost chronic disability, and it is a life-changing experience for family members and societies. For families, the unpredictable nature of the injury leads some of the members into an 'unexpected career' as family caregivers, the caregiver’s have to put enormous efforts, to provide continuous full-time caregiving for the recovery of Person with Spinal cord injury, which will affect the caregiver's physical and mental health. The current study aims to develop and test the feasibility of a well-being programme for caregivers of persons with spinal cord injury. Methods/Design: The current study adopts a Quasi-experimental study design, which have three phases; the first phase is to explore the needs of the caregivers by conducting in-depth interviews with different stakeholders. The second phase is to develop a well-being programme, and checking the feasibility of the programme is the third phase by recruiting 24 caregivers. Qualitative data will be analyzed using thematic analysis, whereas quantitative data will be analyzed using appropriate parametric or non- parametric tests upon confirming normality of data distribution. Discussion: This study would help us to understand the psycho-social issues and unique needs of the caregivers at different time periods. It also gives information about psycho-social interventions and outcome measures for the well-being of the caregivers.
Studies on the dynamics of vacuum encapsulated 2D MEMS scanners by laser Doppler vibrometry

2D MEMS scanners are used for e.g. Laser projection purposes or Lidar applications. Electrostatically driven resonant torsional oscillations of both axes of the scanners lead to Lissajous trajectories for Laser beams reflected from the micro mirror. Wafer level vacuum encapsulation with tilt glass capping ensures high angular amplitudes at low driving voltages additionally preventing environmental impacts. Applying Laser Doppler Vibrometry, the effect of residual gas friction, squeezed film damping and internal friction on 2D MEMS scanners is analyzed by measuring the Q-values associated with the torsional oscillations. Vibrometry is also used to analyze the oscillatory motion of the micro mirror and the gimbal of the scanners. Excited modes of the scanner structures are identified giving rise to coupling effects influencing the scanning performance of the 2D MEMS mirrors.
On-field experience with online diagnosis of large induction motors cage failures using MCSA

The experience gained by ENEL Produzione (previously the Italian Electric Board) on monitoring the cage condition of large induction motors is reported in this paper. The diagnostic procedure is based on the motor current signature analysis and, in particular, on the two sideband current components near the frequency fundamental line that appear in the current power spectrum when a rotor bar/ring breakage occurs. According to the developed procedure, a diagnostic index obtained from these components is stored and its trend as a function of time allows for the detection of the occurrence of a failure in most cases. This event is clearly shown by the overcoming of a prefixed and triggered threshold. Moreover, machines with particular rotor magnetic structure are considered. In this case, unexpectedly high sideband components appear, even in the presence of healthy cages, and the test procedure was adapted to account for these conditions.
Expression of miRNA-25 in young and old lung adenocarcinoma

Background: An appropriate personalized molecular testing ensures the most efficacious treatment in lung cancer. It is still controversial whether younger lung adenocarcinoma (LUAD) patients have different molecular features compared with their older counterparts. MicroRNAs have been involved in lung cancer and their altered expression has been suggested as a potential biomarker in the pathogenesis, diagnosis, prognosis, and therapy of LUAD. Materials and Methods: To analyze putative differences in miR-25 expression between young (with age ≤50 years) and old adenocarcinoma patients, we quantified miR-25 levels with NanoString technology in 88 LUAD specimens. We further investigated a cohort of 309 LUAD patients from the cancer genome atlas (TCGA) database to test our hypothesis. Results: miR-25 expression was upregulated in young LUAD patients in comparison to the older ones (P = 0.03) in our series. The analysis of public database TCGA confirmed our results, which miR-25 differentially expressed in the two aged groups (P = 0.0009). Moreover, a consequential pairing of miR-25 with a target region in phosphatase and tensin homolog (PTEN) 3’ untranslated region (UTR) and actually low PTEN expression seemed to be associated with high miR-25 (P = 0.001) in young patients. Conclusions: The interaction of miR-25 and PTEN in young LUAD may define a subgroup of patients, highlighting the concept of molecular testing in different age subtypes.
On‐Demand Editing of Surface Properties of Microstructures Made by 3D Direct Laser Writing via Photo‐Mediated RAFT Polymerization

Recently, photo‐controlled reversible addition‐fragmentation chain transfer (RAFT) polymerization has been successfully applied in digital light processing 3D printing. It provides a convenient way to tune the surface properties of the 3D printed object. However, so far, 3D micro‐ and nanostructures and reconfigurations based on photo‐induced RAFT polymerization have not been presented. In this work, one macro‐photoiniferter, synthesized by photo‐controlled RAFT polymerization is applied, to 3D direct laser writing. Thanks to the exquisite spatial control of the photoreaction, 3D microstructures with feature sizes of around 500 nm are successfully obtained. Taking advantage of the presence of dormant polymeric RAFT agents, photo‐induced post‐modification of the printed microstructures is highlighted via the elaboration of multi‐chemistry patterns including thermo‐responsive ones. These results open new perspectives in multi‐material and 4D micro‐printing.
Analysis of recorded helicopter echo

The problem of a hovering helicopter detection and classification with a radar system is presented. First, the possible components of a helicopter echo are analyzed. A theoretical model of helicopter rotor backscatter is described, showing the anticipated detection difficulties. An experiment, consisting of radar signal recording and off-line analysis is outlined. The analysis results show the possibility to identify a helicopter echo by time- or frequency-domain characteristics. The probability of detection during normal radar operation is, however, shown to be moderate.
Simplex of HR: Talent Management with Simplex Methodology

This study searches for use of simplex theory in talent management. It is a research topic belonging to this study. Human resource management (HRM) can be described with performance focus and talent management. This study presents a new perspective in talent management. Firstly, Talent management may be described with fulfilling organizational positions by bets talents, because talents further performance of departments and performance of firm. Firm has departments, such as production department, marketing departments, finance department, and etc. This study suggests simplex method for talent management for practitioners. It identifies research question and has two propositions that simplex may be used in talent management. Secondly, study depicts linear of American HRM. It is based on a relationship among human resource (HR) systems, various HRM practices, and organizational performance. Linear proposition of study is that, HRM practices as a system have an impact on firm performance (goal function).
Combination of Wnt/β-Catenin Targets S100A4 and DKK1 Improves Prognosis of Human Colorectal Cancer

Simple Summary Aberrant Wnt/β-catenin signaling contributes to the development, progression, and metastasis of CRC, by altering target gene expression connected to cancer cell proliferation and motility. S100A4 is a Wnt/β-catenin target gene, which strongly enhances migration and invasion of CRC cells and thus CRC metastasis. Here, we report the transcriptional cross-regulation of S100A4 and the Wnt antagonist DKK1, in which the expression of S100A4 down-regulates DKK1 expression, sustaining activated Wnt signaling. S100A4 is an established prognostic biomarker for CRC patient survival, and the combination of S100A4 and DKK1 can be used to improve the prognosis of overall and metastasis-free survival. Abstract Metastasis is directly linked to colorectal cancer (CRC) patient survival. Wnt signaling through β-catenin plays a key role. Metastasis-inducing S100A4 is a Wnt/β-catenin target gene and a prognostic biomarker for CRC and other cancer types. We aimed to identify S100A4-dependent expression alterations to better understand CRC progression and metastasis for improved patient survival. S100A4-induced transcriptome arrays, confirmatory studies in isogenic CRC cell lines with defined β-catenin genotypes, and functional metastasis studies were performed. S100A4-regulated transcriptome examination revealed the transcriptional cross-regulation of metastasis-inducing S100A4 with Wnt pathway antagonist Dickkopf-1 (DKK1). S100A4 overexpression down-regulated DKK1, S100A4 knock-down increased DKK1. Recombinant DKK1 reduced S100A4 expression and S100A4-mediated cell migration. In xenografted mice, systemic S100A4-shRNA application increased intratumoral DKK1. The inverse correlation of S100A4 and DKK1 was confirmed in five independent publicly available CRC expression datasets. Combinatorial analysis of S100A4 and DKK1 in two additional independent CRC patient cohorts improved prognosis of overall and metastasis-free survival. The newly discovered transcriptional cross-regulation of Wnt target S100A4 and Wnt antagonist DKK1 is predominated by an S100A4-induced Wnt signaling feedback loop, increasing cell motility and metastasis risk. S100A4 and DKK1 combination improves the identification of CRC patients at high risk.
Molecular Mechanistic Pathways Targeted by Natural Antioxidants in the Prevention and Treatment of Chronic Kidney Disease

Chronic kidney disease (CKD) is the progressive loss of renal function and the leading cause of end-stage renal disease (ESRD). Despite optimal therapy, many patients progress to ESRD and require dialysis or transplantation. The pathogenesis of CKD involves inflammation, kidney fibrosis, and blunted renal cellular antioxidant capacity. In this review, we have focused on in vitro and in vivo experimental and clinical studies undertaken to investigate the mechanistic pathways by which these compounds exert their effects against the progression of CKD, particularly diabetic nephropathy and kidney fibrosis. The accumulated and collected data from preclinical and clinical studies revealed that these plants/bioactive compounds could activate autophagy, increase mitochondrial bioenergetics and prevent mitochondrial dysfunction, act as modulators of signaling pathways involved in inflammation, oxidative stress, and renal fibrosis. The main pathways targeted by these compounds include the canonical nuclear factor kappa B (NF-κB), canonical transforming growth factor-beta (TGF-β), autophagy, and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid factor 2-related factor 2 (Nrf2)/antioxidant response element (ARE). This review presented an updated overview of the potential benefits of these antioxidants and new strategies to treat or reduce CKD progression, although the limitations related to the traditional formulation, lack of standardization, side effects, and safety.
Evaluating the Performance of Two Alternative Geographic Surveillance Schemes

32.3% 37.4% 41.3% The table above shows the percentage of Medicaid cases detected during the simulation for each model and for each probability function. The 19 sites calculated de novo using the K-median model capture 3.3% more cases than the 19 existing sites when using the exponentially decreasing probability function based on distance. Additionally, using the commonly used Huff probability model, the same sites achieve 9% better detection than the existing sites. While the sites calculated using the MCM detect fewer cases than the existing sites using the distance-based probability function, they capture 5.1% more cases using the Huff model.
Vaccine Hesitancy in the Time of COVID-19: Attitudes and Intentions of Teens and Parents Regarding the COVID-19 Vaccine

To assess attitudes and intentions related to the COVID-19 vaccine during the pandemic, we surveyed adolescents aged 13–18 years and the parents of 13–18-year-olds using national research panels on three occasions or “waves”: before the COVID-19 vaccine was available, after it was available for adults, and after it was available for ages ≥12 years. Data on experiences with COVID-19, the importance of adolescent vaccines, and intentions regarding COVID-19 vaccination were analyzed across time points. We found that parental concerns about vaccine safety significantly increased from Wave 1 to 2. Social media had a negative influence on parents’ and adolescents’ opinions about vaccine safety. Demographic variables were associated with vaccination rates reported in Wave 3, consistent with known inequities related to vaccine access. Parents (70%) were supportive of concomitant COVID-19 vaccination with other adolescent vaccines for teens. It is important to address variables associated with vaccine hesitancy to increase COVID-19 vaccine coverage rates in the US.
A taxonomic outline of the Poecilimon affinis complex (Orthoptera) using the geometric morphometric approach

The genus Poecilimon contains 145 species, widely distributed in the Palaearctic, among which the Poecilimon ornatus group has the greatest diversity in the Balkans. Despite several revisions of the genus, the systematics of the species group, and in particular, of the taxa associated with the species Poecilimon affinis, is still unsolved. Due to morphological similarity, P. affinis with its subspecies, P. nonveilleri and P. pseudornatus form the Poecilimon affinis complex. The aim of this study is to test the hypotheses of an outlined species complex, namely the P. affinis complex, within the P. ornatus group using morphological data. Geometric analysis was conducted to explore variation in the structure of the male tegmen, ovipositor, male cercus, and male pronotum. The number of teeth and stridulatory file measurements provided additional information on morphological variation within the complex. A phylogenetic tree based on the cytochrome c oxidase subunit I gene (COI) was used for comparison with the morphological data. Canonical variate analysis showed that male tegmen and male cercus are good morphostructures to distinguish the taxa belonging to the P. affinis complex from other species in the P. ornatus group. This may confirm our assumption for the designation of the P. affinis complex. The results of the principal component analysis of stridulatory file measurements, molecular data, and CVA of the ovipositor suggest adding two additional species to the complex: P. ornatus and P. hoelzeli.