EFFECT OF CHINESE MEDICAL HERBS-HUIRU ON HYPERPROLACTINEMIA AND HYPERPLASIA OF MAMMARY GLAND IN MICE

The study investigated the pharmacodynamism and mechanism of Chinese medicinal formula-Huiru Yizeng Yihao (NO.1 HRYZ) on the model rats of hyperpro-lactinemia and the model rats of hyperplasia of mammary gland (HMG), and studied the internal connection between hyperprolactinemia and HMG. The hyperprolactinemia rat models were established by injecting metoclopramide dihydrochloride in the back of rats. The model rat of HMG was prepared by injecting estradiol in the thigh muscle of the rats and progesterone consecutively, while the tails of rats were clipped with tongs. Rats were treated with either NO.1 HRYZ or positive control drugs for four weeks. The concentrations of sex hormone in rat serum were examined using ELISA kits, and the morphology of mammary gland tissue in all group rats was observed with microscope. NO.1 HRYZ significantly decreased prolactin (PRL) and increased estradiol (E 2 ), progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH) concentrations of hyperprolactinemia rats. It decreased E 2, PRL, FSH, gonadotropin-releasing hormone (GnRH), 5-hydroxytryptamine (5-HT) and increased P concentrations of HMG rat. It also eliminated hyperplasia of lobules and gland alveolus compared with the model group. Treatment with NO.1 HRYZ could significantly regulate the sex hormone disorder of hyperprolactinemia and HMG rat models, and could eliminate the formation of HMG. Hyperprolactinemia was closely correlated with HMG, and hyperprolactinemia promoted the formation of HMG. HRYZ inhibits chronic proliferative inflammation processes with a dose-dependent inhibition of granuloma formation in rat, and also has protective and therapeutic effects on HMG rats induced by oestrogen and progestogen. After injection of oestrogen and progestogen, results showed that E2, PRL, FSH, GnRH and 5-HT were remarkably decreased and P concentration was significantly increased by NO.1 HRYZ (31.6g/kg or 15.8g/kg) treatment compared with HMG model group. Histopathologic examination of NO.1 HRYZ (31.6g/kg) treatment remarkably alleviated the degree of HMG, number of lobules, number of acinars and lobule volumes decreased in different degrees. Therefore, the mechanism of NO.1 HRYZ in treating HMG is by way of regulating endocrinal and immune functions to balance hormonal levels in the body to inhibit the pathological proliferation of mammary gland. In our experiment, we found that the serum PRL increased and E 2 , P, FSH and LH decreased in hyperprolactinemia rat models. And the serum PRL, E 2 , FSH, GnRH, 5-HT increased and P decreased in HMG rat models. Although the hormone disorders were different, NO.1 HRYZ could regulate the hormone disorder of hyperprolactinemia and HMG model rats, illustrating that Chinese medical herbs had effective balance function. NO.1 HRYZ could regulate the disorder of hypothalamus-pituitary-gonadal axis in hyperprolactinemia and HMG model rats and could make sex hormones return to normal, thus treating them effectively. high-dosage, middle-dosage and low-dosage of NO.1 HRYZ in searching for the best dosage. middle-dosage of NO.1 HRYZ there be some reactions in high-dosage NO.1 HRYZ for reducing effect, high-dose of NO.1 HRYZ less effect on E2 concentration than middle-dose group. middle-dosage of NO.1 HRYZ acted best curative high-dose group and middle-dose group have same effects on FSH and LH even with the increased dosage of NO.1 HRYZ. are still