Miracle-dz/UniFAR-FTUs huggingface dataset:
The cross-sectional, longitudinal, and transitional associations between perceived support and suicidal ideation and behavior in late adolescence and emerging adulthood: Adjacent-category logit models.

INTRODUCTION Suicidal ideation (SI) and attempts (SA) are prevalent in late adolescence and emerging adulthood. Prior research has identified perceived support as a correlate of SI and SA. Less is known, though, about the role of perceived support in differentiating among suicidal outcomes and between the incremental escalation of suicidal outcomes from SI to SA to serious suicide attempts (SSA). METHOD Using ordinal regression, we used Wave 1 and 2 data from the National Longitudinal Study of Adolescent to Adult Health (N = 4500; 53% female; Mage = 16.6; 12% Hispanic) to examine cross-sectional, longitudinal, and transitional models of perceived support and suicidality. RESULTS Cross-sectional results indicated that youths with higher perceived support were less likely to have suicidal ideation. Longitudinal results showed that youths with higher perceived support at Wave 1 were less likely to have suicidal ideation or serious suicide attempts at Wave 2. Transitional model results revealed that higher perceived support at Wave 1 was negatively associated with escalation in suicidal outcomes from Wave 1 to 2. CONCLUSION We discuss findings in the context of theories of suicide and discuss implications for suicide risk identification, intervention, and prevention efforts in late adolescence and emerging adulthood.
Association of Coexistent Hepatitis B Surface Antigen and Antibody With Severe Liver Fibrosis and Cirrhosis in Treatment-Naive Patients With Chronic Hepatitis B

Key Points Question Is the coexistence of hepatitis B surface antigen (HBsAg) and antibody against HBsAg (anti-HBs) associated with severe liver fibrosis and cirrhosis in patients with chronic hepatitis B (CHB)? Findings In this cross-sectional study of 6534 patients with CHB, the coexistence of HBsAg and anti-HBs was independently associated with severe liver fibrosis and cirrhosis in patients with CHB, especially among those who had hepatitis B e antigen negativity. Meaning This study’s findings suggest that coexistent HBsAg and anti-HBs is a rare serological pattern that may reflect a special status of infection and that close monitoring for liver fibrosis and cirrhosis is warranted in patients with CHB who have this serological profile.
Genetic diversity, gene flow and differentiation among wild, semiwild and landrace chile pepper (Capsicum annuum) populations in Oaxaca, Mexico.

PREMISE Capsicum annuum (Solanaceae) was originally domesticated in Mexico, where wild (C. annuum var. glabriusculum) and cultivated (C. annuum var. annuum) chile pepper populations (> 60 landraces) are common; additionally wild-resembling individuals (hereafter semiwild) grow spontaneously in anthropogenic environments. Here we analyze the role of elevation and domestication gradients in shaping the genetic diversity in C. annuum from the state of Oaxaca, Mexico. METHODS We collected samples of 341 individuals from 28 populations, corresponding to wild, semiwild (C. annuum var. glabriusculum) and cultivated C. annuum, and closely related species Capsicum frutescens and C. chinense. From the genetic variation of 10 simple sequence repeat (SSR) loci we assessed the population genetic structure, inbreeding and gene flow through variance distribution analyses, genetic clustering and connectivity estimations. RESULTS Genetic diversity (HE ) did not differ across domestication levels. However, inbreeding coefficients were higher in semiwild and cultivated chiles than in wild populations. We found evidence for gene flow between wild populations and cultivated landraces along the coast. Genetic structure analysis revealed strong differentiation between most highland and lowland landraces. CONCLUSIONS Gene flow between wild and domesticated populations may be mediated by backyards and small-holder farms, while mating systems may facilitate gene flow between landraces and semiwild populations. Domestication and elevation may overlap in their influence on genetic differentiation. Lowland Gui'ña dani clustered with highland landraces perhaps due to social history of Zapotec peoples. In situ conservation may play an important role in preserving semiwild populations, and private alleles found in landraces. This article is protected by copyright. All rights reserved.
Cysteine and glycine‐rich protein 2 promotes hypoxic pulmonary vascular smooth muscle cell proliferation through the Wnt3α‐β‐catenin/lymphoid enhancer‐binding factor 1 pathway

Pulmonary hypertension (PH) is mainly characterized by abnormal pulmonary vascular hyperplasia and vascular remodeling, but its mechanism is complicated and currently unclear. Cysteine and glycine‐rich protein 2 (Csrp2) has been reported to promote cell proliferation and migration, and affect cell cycle progression. As a new invasive actin‐binding factor, Csrp2 increased the invasion and even metastasis of some cancer cells. It was associated with tumor recurrence and chemotherapy resistance. However, the role of Csrp2 in PH remains unknown. We found that Csrp2 expression was increased both in pulmonary arteries (PAs) and smooth muscle cells (PASMCs) in PH. Csrp2 enhanced PASMC proliferation and phenotypic transition. The Wnt3α‐β‐catenin/lymphoid enhancer‐binding factor 1 (LEF1) pathway is involved in cell proliferation and phenotypic transition regulated by Csrp2 expression. These results suggest that hypoxia downregulates YinYang‐1 (YY1) and then increases Csrp2 expression. Increased Csrp2 promotes PASMC proliferation and phenotypic transition by activating the Wnt3α‐β‐catenin/LEF1 pathways, which leads to pulmonary vascular remodeling and even provides a new theoretical basis for studying the pathogenesis and therapeutic targets of PH.
Deoxynivalenol and its modified forms: key enzymes, inter-individual and interspecies differences in metabolism

Abstract Deoxynivalenol (DON) and its modified forms, including DON-3-glucoside (DON-3G), pose a major agricultural and food safety issue in the world. Their metabolites are relatively well-characterized; however, their metabolizing enzymes have not been fully explored. UDP-glucuronosyltransferases, 3-O-acetyltransferase, and glutathione S-transferase are involved in the formation of DON-glucuronides, 3-acetyl-DON, and DON-glutathione, respectively. There are interindividual differences in the metabolism of these toxins, including variation with respect to sex. Furthermore, interspecies differences in DON metabolism have been revealed, including differences in the major metabolites of DON, the role of de-acetylation, and the hydrolysis of DON-3G. In this review, we summarized the major enzymes involved in metabolizing DON to its modified forms, focusing on the differences in metabolism of DON and its modified forms between individuals and species. This work provides important insight into the toxicity of DON and its derivatives in humans and animals, and provides scientific basis for the development of safer and more efficient biological detoxification methods.
First Report of the Colistin Resistance Gene mcr-10.1 Carried by IncpA1763-KPC Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone

Colistin is used as one of the last available choices of antibiotics for patients infected by carbapenem-resistant bacterial strains, but the unrestricted use of colistin aggravated the acquisition and dissemination of mobile colistin resistance (mcr) genes. So far, 10 mcr genes have been reported in four continents around the world. ABSTRACT Mobile colistin resistance (mcr) gene mcr-10.1 has been distributed widely since it was initially identified in 2020. The aim of this study was to report the first mcr-10.1 in Africa and the first mcr in Sierra Leone; furthermore, we presented diverse modular structures of mcr-10.1 loci. Here, the complete sequence of one mcr-10.1-carrying plasmid in one clinical Enterobacter cloacae isolate from Sierra Leone was determined. Detailed genetic dissection and comparison were applied to this plasmid, together with a homologous plasmid carrying mcr-10.1 from GenBank. Moreover, a genetic comparison of 19 mcr-10.1 loci was performed. In this study, mcr-10.1 was carried by an IncpA1763-KPC plasmid from one Enterobacter cloacae isolate. A total of 19 mcr-10.1 loci displayed diversification in modular structures through complex transposition and homologous recombination. A site-specific tyrosine recombinase XerC was located upstream of mcr-10.1, and at least one insertion sequence element was inserted adjacent to a conserved xerC-mcr-10.1-orf336-orf177 region. Integration of mcr-10.1 into a different gene context and carried by various Inc plasmids contributed to the wide distribution of mcr-10.1 and enhanced the ability of bacteria to survive under colistin selection pressure. IMPORTANCE Colistin is used as one of the last available choices of antibiotics for patients infected by carbapenem-resistant bacterial strains, but the unrestricted use of colistin aggravated the acquisition and dissemination of mobile colistin resistance (mcr) genes. So far, 10 mcr genes have been reported in four continents around the world. This study presented one mcr-10.1-carrying Enterobacter cloacae isolate from Sierra Leone. The mcr-10.1 gene was identified on an IncpA1763-KPC plasmid. According to the results of genetic comparison of 19 mcr-10.1 loci, the mcr-10.1 gene was found to be located in a conserved xerC-mcr-10.1-orf336-orf177 region, and at least one insertion sequence element was inserted adjacent to this region. To our knowledge, this is the first report of identifying the mcr-10.1 gene in Africa and the mcr gene in Sierra Leone.
The Nature of Word Associations in Sentence Contexts

Abstract. How words are interrelated in the human mind is a scientific topic on which there is still no consensus, with different views on how word co-occurrence and semantic relatedness mediate word association. Recent research has shown that lexical associations are strongly predicted by the similarity of those words in terms of valence, arousal, and concreteness ratings. In the current study, we aimed at extending these results to more complex and realistic linguistic scenarios, since human communication is not done with word pairs, but rather through sentences. Hence, the aim of the current study was to verify whether valence, arousal, and concreteness also articulate sentence-level lexical representations. To this end, 32 native Spanish speakers were given cue words and asked to use them in sentences that would provide a meaningful context. The content words of the written sentences were then analyzed. Our results showed that the emotional dimensions (valence and arousal) and concreteness values of the cue words effectively predicted the same values of said dimensions of their sentences’ words. In sum, the similarity in the emotional dimensions and concreteness are crucial mechanisms behind word association in the human mind.
Reconstitution of Mycobacterium marinum Nonhomologous DNA End Joining Pathway in Leishmania

Nonhomologous end joining (NHEJ) is the most efficient double-stranded DNA break (DSB) repair pathway in mammalian cells. In contrast, the protozoan parasite Leishmania has no functional NHEJ pathway but retains the core NHEJ factors of Ku70 and Ku80 proteins. ABSTRACT In mammalian cells, DNA double-strand breaks (DSBs) are mainly repaired by nonhomologous end joining (NHEJ) pathway. Ku (a heterodimer formed by Ku70 and Ku80 proteins) and DNA ligase IV are the core NHEJ factors. Ku could also be involved in other cellular processes, including telomere length regulation, DNA replication, transcription, and translation control. Leishmania, an early branching eukaryote and the causative agent of leishmaniasis, has no functional NHEJ pathway due to its lack of DNA ligase IV and other NHEJ factors but retains Ku70 and Ku80 proteins. In this study, we generated Leishmania donovani Ku70 disruption mutants and Ku70 and Ku80 double gene (Ku70/80) disruption mutants. We found that Leishmania Ku is still involved in DSB repair, possibly through its binding to DNA ends to block and slowdown 5′ end resections and Ku-Ku or other protein interactions. Depending on location of a DSB between the direct repeat genomic sequences, Leishmania Ku could have an inhibiting effect, no effect or a promoting effect on the DSB repair mediated by single strand annealing (SSA), the most frequently used DSB repair pathway in Leishmania. Ku70/80 proteins are also required for the healthy proliferation of Leishmania cells. Interestingly, unlike in Trypanosoma brucei and L. mexicana, Ku70/80 proteins are dispensable for maintaining the normal lengths of telomeres in L. donovani. We also show it is possible to reconstitute the two components (Ku and Ligase D) NHEJ pathway derived from Mycobacterium marinum in Leishmania. This improved DSB repair fidelity and efficiency in Leishmania and sets up an example that the bacterial NHEJ pathway can be successfully reconstructed in an NHEJ-deficient eukaryotic parasite. IMPORTANCE Nonhomologous end joining (NHEJ) is the most efficient double-stranded DNA break (DSB) repair pathway in mammalian cells. In contrast, the protozoan parasite Leishmania has no functional NHEJ pathway but retains the core NHEJ factors of Ku70 and Ku80 proteins. In this study, we found that Leishmania Ku heterodimers are still participating in DSB repair possibly through blocking 5′ end resections and Ku-Ku protein interactions. Depending on the DSB location, Ku could have an inhibiting or promoting effect on DSB repair mediated by the single-strand annealing repair pathway. Ku is also required for the normal growth of the parasite but surprisingly dispensable for maintaining the telomere lengths. Further, we show it is possible to introduce Mycobacterium marinum NHEJ pathway into Leishmania. Understanding DSB repair mechanisms of Leishmania may improve the CRISPR gene targeting specificity and efficiency and help identify new drug targets for this important human parasite.
Comorbid allergic rhinitis and asthma: important clinical considerations

ABSTRACT Introduction The numerous links between allergic rhinitis and asthma have been extensively explored in the last two decades, gaining great concern within the scientific community. These two conditions frequently coexist in the same patient and share numerous pathogenetic and pathophysiological mechanisms. Areas covered We reviewed major pathophysiological, epidemiological, and clinical links between allergic rhinitis and asthma. We also provided a comprehensive discussion of allergic rhinitis treatment according to current guidelines, with a particular focus on the relevance of allergic rhinitis therapies in patients with comorbid asthma. Expert opinion We believe that there are several unmet needs for our patients, however, there are promising advances forecasted for the future. Although allergic rhinitis is a recognized risk factor for asthma, a proper asthma detection and prevention plan in allergic rhinitis patients is not available. Allergen immunotherapy (AIT) represents a promising preventive strategy and may deserve an earlier positioning in allergic rhinitis management. A multidisciplinary approach should characterize the journey of patients with respiratory allergies, with an adequate referral to specialized Allergy/Asthma centers. Molecular Allergy Diagnosis may provide support for optimal AIT use. Finally, a possible evolution of biological treatment can be envisaged, mainly if biosimilars decrease such therapies’ costs.
Psychometric properties of the Teenage Executive Functioning Inventory in a Turkish sample of adolescents: deficits in working memory and inhibition among adolescents

ABSTRACT The Teenage Executive Functioning Inventory (TEXI) is a measurement tool that consists of two subscales, working memory and inhibition, and allows both self and parent rating. This study aimed to examine the psychometric properties of the Turkish version of TEXI in a sample of 184 parents and 476 adolescents aged 11 and 18 years and to measure the relationship between executive functions and mental health problems, including attention deficit hyperactivity disorder and major depressive disorder and anxiety disorders. Confirmatory factor analyses showed acceptable/good fit indices and confirmed the two-factor structure for both the adolescent and parent forms. Test-retest results and Cronbach’s alpha coefficient showed that the scale had high reliability. The results also showed that deficits in executive functions were associated with various mental health problems in adolescents. The findings demonstrated that a Turkish translation of the TEXI is a valid and reliable tool for evaluating the executive functions of adolescents.
The beauty of diversity in cognitive neuroscience: The case of sex‐related effects in language production networks

Over the past few decades, several studies have focused on potential sex‐related differences in the trajectories of language development and functioning. From a behavioral point of view, the available literature shows controversial results: differences between males and females in language production tasks may not always be detectable and, even when they are, are potentially biased by sociological and educational confounding factors. The problem regarding potential sex‐related differences in language production has also been investigated at the neural level, again with controversial results. The current minireview focuses on studies assessing sex‐related differences in the neural networks of language production. After providing a theoretical framework of language production, it is shown that the few available investigations have provided mixed results. The major reasons for discrepant findings are discussed with theoretical and methodological implications for future studies.
Correlates of Moderate-to-Vigorous Physical Activity in Children With Physical Illness and Physical–Mental Multimorbidity

This study measured physical activity (PA) and explored its correlates among children with multimorbidity (co-occurring chronic physical and mental illness; MM) versus those with chronic physical illness only (PI). This study used baseline data from the Multimorbidity in Children and Youth Across the Life Course (MY LIFE) study, an on-going cohort study following 263 children with a PI 2 to 16 years of age (mean age: 9.8 years, SD = 4.0; 47.7% female). PA was measured using accelerometry, and demographic and psychosocial variables were collected using questionnaires. Of the 55 children with MM and the 85 with PI with valid accelerometer data, 38.1% and 41.2%, respectively, met average daily PA guidelines. Correlates of moderate-to-physical PA (MVPA) among children with MM were age, ρ(53) = −0.45, p = . 001, body mass index (BMI), ρ(48) = −0.28, p = . 04, self-perceived behavioral conduct, ρ(24) = −0.45, p = . 02, physical health-related quality of life, ρ(51) = 0.56, p < . 001, and peer support, ρ(52) = 0.27, p = . 04. Correlates of MVPA among children with PI were age, ρ(83) = −0.40, p < . 001, sex, ρ(83) = −0.26, p = . 01, self-perceived social competence, ρ(31) = 0.42, p = . 02, self-perceived athletic competence, ρ(31) = 0.48, p = . 005, physical health-related quality of life, ρ(83) = 0.34, p = . 001, participation in community sport, ρ(31) = 0.41, p = . 02, and family functioning, ρ(83) = 0.26, p = . 02. These results demonstrate that children with PI and MM are insufficiently active and their PA is correlated with demographic and psychosocial factors.
Can Diffusion Tensor Imaging Apparent Diffusion Coefficient Diagnose Carpal Tunnel Syndrome? A Systematic Review and Meta-Analysis

Magnetic resonance diffusion tensor imaging (DTI) can detect microstructural changes in peripheral nerves. Studies have reported that the median nerve apparent diffusion coefficient (ADC), a quantification of water molecule diffusion direction, is sensitive in diagnosing carpal tunnel syndrome (CTS). Five databases were searched for studies using ADC to investigate CTS. A﻿pparent diffusion coefficient (measured in mm2/s) were pooled in random-effects meta-analyses. Twenty-two studies met criteria yielding 592 patients with CTS and 414 controls. Median nerve ADC were measured at the level of the distal radioulnar joint (CTS ADC: 1.11, 95% CI: 1.07-1.15, I2 = 54%; control ADC: 1.04, 95% CI: 1.01-1.07, I2 = 57%), pisiform (CTS ADC: 1.39, 95% CI: 1.37-1.42, I2 = 0%; control ADC: 1.27, 95% CI: 1.23-1.31, I2 = 59%), hamate (CTS ADC: 1.40, 95% CI: 1.36-1.43, I2 = 58%; control ADC: 1.27, 95% CI: 1.25-1.28, I2 = 47%), and as an combination of several measurements (CTS ADC: 1.40, 95% CI: 1.37-1.47, I2 = 100%; control ADC: 1.39, 95% CI: 1.24-1.53, I2 = 100%). Median nerve ADC is decreased in individuals with CTS compared to controls at the levels of the hamate and pisiform. ADC cut-offs to diagnose CTS should be established according to these anatomic levels and can be improved through additional studies that include use of a wrist coil.
Fresh news as meaningful? A multi‐site ethnographic analysis of meaningful activities at four day centres in Denmark and Norway

Abstract Day centres are increasingly being established, as many older persons are isolated and in need of meaningful activities and social interaction with others. Previous research has shown that day centres are still an important arena for older attendees to socialise and engage in meaningful activities, although day centres are increasingly introducing activities as part of rehabilitation programmes to enhance physical and mental enablement. However, little is known about what attendees and staff regard as meaningful activities. Based on a multi‐site ethnographic investigation at four day centres in Denmark and Norway in 2018 and 2019, this article examines how staff and attendees alike ‘utilise’ day care centres to pursue meaningful activities and what is considered meaningful for those attending and working there. Furthermore, this article discusses the potential for person‐centred care in communities like day centres. Our study shows that activities are first and foremost perceived as meaningful if they enhance an enjoyable social dimension with ‘a touch of fresh news’. Hence, day centres function as a social space where elderly attendees can share stories and news based on personal experiences from the past and present. Consequently, person‐centred care in day care centres preferably facilitates communities to give attendees something new and refreshing to bring back home with them—and not only facilitate personal histories, preferences and wishes.
Quo vadis geriatric rehabilitation?

Abstract The sustainability of healthcare of older people in Europe is at stake. Many experts currently focus on the COVID-19 pandemic and its consequences. But there are other elements coming up that might even have a greater impact. Healthcare systems, geriatric care and geriatric rehabilitation in particular, will face disruptive changes due to both demographic demand and a shortage of human and financial resources. This decade will be transformed by a high proportion of the older health workforce transitioning to retirement. This expertise must be retained. The brain drain of health care workers migrating from Eastern parts to Western Europe is diminishing. Discussing and deciding upon the priorities of value-based health care for older people such as equity and access is required. The acute healthcare sector in most countries focuses on fee-for-service models instead of building systemic approaches to maximise independence and autonomy of older citizens. In this commentary, we build on recent book chapters and articles on geriatric rehabililtation. Our main questions for the anniversary edition of Age and Ageing is what it is that geriatric rehabilitation could, should and must contribute in the roaring 2020s?