Miracle-dz/UniFAR-FTUs huggingface dataset:
Modifications électrochimiques de surfaces et dispositifs électroniques organiques

L’electronique organique reste un domaine de recherche prolifique grâce a la diversite de structures moleculaires accessible par la synthese organique. Les materiaux moleculaires offrent des possibilites de mises en forme inedites comme les techniques de depot en solution, utilisables dans la conception de dispositifs organiques sur supports plastiques. Nos travaux de recherche melent electrochimie, conception et caracterisations electriques de dispositifs et mesures capteur. Ils abordent deux thematiques : l’elaboration de nouveaux transducteurs a base de polyanilines substituees et de phtalocyanines et l’etude de l’influence de la modification electrochimique de surface sur le comportement de dispositifs connus.Nous avons tout d’abord elabore des heterojonctions laterales polymere – phtalocyanine en utilisant les proprietes des polyanilines. De par leur geometrie, ces nouveaux dispositifs different de l’heterojonction MSDI (Molecular Semiconductor – Doped Insulator), transducteur conductimetrique bicouche developpe au laboratoire pour la detection de gaz comme l’ozone ou l’ammoniac. La caracterisation complete de la poly(2,3,5,6-tetrafluoroaniline) indique que ce polymere est peu conducteur, en comparaison de la polyaniline dont la conductivite peut etre augmentee par dopage acido-basique. La presence des fluors empeche l’emergence du regime conducteur que l’on retrouve en milieu acide pour la polyaniline et la poly(2,5-dimethoxyaniline). Ces trois polymeres, electrodeposes sur electrodes d’ITO interdigitees ont permis, apres sublimation de la bisphtalocyanine de lutecium, de construire les heterojonctions laterales. Le comportement electrique des differents dispositifs, etudie notamment par spectroscopie d’impedance, differe en fonction de la nature des substituants de la polyaniline electrodeposee. Enfin, des mesures capteur ont montre la possibilite de detecter l’ammoniac en milieu humide, avec une limite de detection sub ppm.De precedents travaux sur l’elaboration de MSDI ont souligne le role primordial des interfaces, notamment dans le cas des n-MSDI qui presentent une heterojonction p-n. Outre le changement de la nature chimique de la sous-couche employee, une autre maniere de jouer sur les interfaces est de modifier electrochimiquement la surface des electrodes interdigitees, par reduction de sels de diazonium. Nous avons ainsi greffe differents benzenes substitues, dont certains ont conduit a la formation de multicouches comme l’ont revelee des mesures de microbalance a quartz electrochimique. Les differentes modifications de surface ont surtout joue le role de barriere isolante, amplifiant le comportement non lineaire des caracteristiques courant-tension des MSDI. Le greffage du 2,5-dimethoxybenzene a permis d’ameliorer significativement la sensibilite a l’ammoniac de la MSDI a base d’hexadecafluorophtalocyanine de cuivre et de bisphtalocyanine de lutetium, avec une limite de detection de l’ordre de 200 ppb.
Cargo Specific Regulation of Cytoplasmic Dynein by Effector Proteins

Axonal transport is vital for the development and survival of neurons. The transport of cargo and organelles from the axon to the cell body is driven almost completely by the molecular motor, cytoplasmic dynein. Yet, it remains unclear how dynein is spatially and temporally regulated given the variety of cargo that must be properly localized to maintain cellular function. Previous work has suggested that adaptor proteins provide a mechanism for cargo-specific regulation of motors. During my thesis work, I have investigated the role of mammalian Hook proteins, Hook1 and Hook3, as potential motor adaptors. Using optogenetic and single molecule assays, I found that Hook proteins interact with both dynein and dynactin, to effectively activate dynein motility, inducing longer run lengths and higher velocities than the previously characterized dynein activator, BICD2. In addition, I found that complex formation requires the N-terminal domain of Hook proteins, which resembles the calponin-homology domain of EB proteins yet cannot bind directly to microtubules. In collaborative studies, we found the Hook domain directly interacts with a helix of the dynein light intermediate chain and this interaction is important for Hook-induced processive motility of dynein. In my final project, I found that Hook1 mediates the transport of TrkB-BDNF signaling endosomes in primary hippocampal neurons. Using live cell microscopy and microfluidic devices, Hook1 depletion resulted in a significant decrease in the flux and processivity of BDNF-Qdots along the mid-axon, an effect specific for Hook1 but not Hook3. Together, my work suggests that dynein effectors like Hook proteins can differentially regulate dynein to allow for organelle-specific tuning of the motor for precise intracellular trafficking. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Biochemistry & Molecular Biophysics First Advisor Erika L. Holzbaur Subject Categories Biochemistry | Biophysics | Cell Biology This dissertation is available at ScholarlyCommons: https://repository.upenn. edu/edissertations/3167 CARGO SPECIFIC REGULATION OF CYTOPLASMIC DYNEIN BY EFFECTOR PROTEINS Mara Alizabeth Olenick
PENGARUH KONSENTRASI TEPUNG PEPAYA DAN LAMA PENYIMPANAN TERHADAP KETAHANAN L. acidophilus FNCC 0051 TERIMOBIL PADA KONDISI ASAM LAMBUNG DAN GARAM EMPEDU

Papaya powder added as a prebiotic, but its concentration and storage time in UHT milk as a carrier can impact on viability of immobilized cell in gastric acid and bile salt condition. The purpose of this study was to determine the effect of the interaction of papaya powder concentration and storage time and also the influence of each these factors on the survival of immobilized L. acidophilus FNCC 0051 in gastric acid and bile salt. The research design used a Randomized Block Design (RBD) of factorial design. Papaya powder concentrations as the first factor consisting of two levels (3% b/v and 6% b/v) and the storage times as the second factor consisting of five levels (0, 7, 14, 21, and 28 days). Each treatment combinations repeated three times. Data were analyzed by ANOVA (Analysis of Variance) at α = 5%. Based on the results, use of 6% concentration papaya powder significantly increasing the survival of immobilized L. acidophilus FNCC 0051 on condition of gastric acid. Meanwhile, interaction of papaya powder concentration and storage time significantly affect the resistance of immobilized L. acidophilus FNCC 0051 on bile salt condition. Storage along 14 days decreasing the cells number, but the survival cell on bile salt condition increased by the use of 6% concentration of papaya powder during 21 days of storage. The highest survival with decreased cell 0,1459 log cfu/g obtained from combination of 6% concentration of papaya powder at 21 days of storage time.
Electron impact scattering study on chlorobenzene

ABSTRACT In this article, we report a detailed theoretical study on electron interactions with chlorobenzene with incident energies ranging from 0.1 to 5000 eV. Due to this wide range of impact energy, we are able to investigate a variety of processes and report data on resonances, differential cross-sections (DCS), momentum transfer cross-sections (MTCS), rate coefficient, vertical electronic excitations, dissociative electron attachment (DEA), total ionisation cross-sections and total scattering cross-sections (TCS). In order to compute the total scattering cross-sections, we have employed the ab-initio R-matrix method via Quantemol-N for the lower energy range (0.1 eV to 20 eV) and Spherical Complex Optical Potential (SCOP) beyond threshold energy of the target to 5000 eV. The R-matrix calculations are performed using a close-coupling method with the aid of 34 target states, 1667 configuration state functions, and 233 channels employing Configuration Interaction (CI) model. The present study reports evidence for electron scattering resonances through analysis of eigenphase diagrams fitting them to Breit-Wigner profile at low energies below the ionisation threshold. GRAPHICAL ABSTRACT
Chemical composition and antioxidant activity of seven different grape extracts

Grape extracts have strong antioxidant potential which can make them promising anticancer and food-supplement. However, one of the main obstacles is that there is not enough data revealing content of the grape extracts. Another important issue is that grape extracts are mostly obtained in methanol and ethanol solvent, which can limit their effective usage. Therefore, in this study, extracts of seven different grape species of white and red grape (which were collected from Kırşehir-Toklumen) were collected using 70:30 water:ethanol, which underwent GC-EI-MS characterization. GC-MS based characterization revealed that the species had key content differences and similarities, which can trigger further research to quantify the content to reveal what exactly brought the differences in the obtained antioxidant potential of the grape species.
Günümüz ve Gelecekte Parkinson Hastalığı için Farmakolojik Tedavi Yaklaşımları

Parkinson hastaligi, nigrostriatal yolakta dopaminerjik noronlarin kay¬bina bagli olarak olusan, tremor, bradikinezi, rijidite ve postural denge bozukluklari ile karakterize, kronik ilerleyici norodejeneratif bir bozuk¬luktur. Parkinson hastaligi dunya genelinde 5 milyon kisiyi etkilemekte ve en yaygin ikinci norodejeneratif bozukluk olarak bilinmektedir. Şu an mevcut tedaviler, norodejeneratif sureci degistirmeye degil, semp¬tomatik tedaviler ile yasam kalitesini arttirmaya yoneliktir. Levodopa Parkinson hastaliginin motor semptomlarinin tedavisinde en etkili ilac olmasina ragmen, bir sure sonra diskinezi ve motor dalgalanmalara sebep olmasi nedeniyle, ya levadopa dozunun degistirilmesi ya da tedaviye diger ilaclarin (dopamin agonistleri, katekol-O-metiltransferaz inhibitorleri, monoamin oksidaz tip B inhibitorleri gibi) eklenmesi gerek¬mektedir. Bu sebeple konvansiyonel tedavilere alternatif olarak yeni tedavi yaklasimlari gelistirilmeye baslanmistir. Bu yaklasimlar, genellikle diskinezi ve motor dalgalanmalari iyilestirmeye yonelik semptomatik olup, bunlarin yani sira norotrofik faktorler ve hucre transplantasyonu gibi henuz baslangic asamasinda olan, onarici yaklasimlarda yer almak¬tadir. Semptomatik tedavi yaklasimlari; derin beyin stimulasyonu, sub¬kutan apomorfin, karbidopa/levodopa intestinal jel, the Accordion Pill®, uzatilmis salimli amantadin, adenozin reseptor antagonistleri, metabot¬ropik glutamat reseptor antagonistleri ve gen tedavisidir. Bu makalede yukarida sozu edilen terapotik yaklasimlarin olumlu ve olumsuz yonleri ele alinmistir. Bu yaklasimlardan bir kismi yakin zamanda kullanilmaya baslanmis, bir kismi ise halen klinik arastirma asamasindadir. Tum bu yaklasimlar Parkinson hastalari icin yeni umut kaynagi olusturmaktadir.
Stabilization of Food Colloids: The Role of Electrostatic and Steric Forces

The role that some forces exert on food colloid stability is discussed. The focus is on the combination of different energy terms, determining particle-particle attraction or repulsion. The forces are relevant in dispersion stabilization and macroscopic phase separation. The observed features depend on the energies at work and colloid concentration. Examples deal with food manipulations giving cheese, yogurt, and mayonnaise. All products result from the overlapping of forces jointly leading to aggregation or phase separation in foods. The combination of attractive, van der Waals (vdW), and repulsive, double-layer (DL) forces results in the dominance of aggregation or dispersion modes, depending on the particle concentration, on the force amplitude, and on their decay length. DL and vdW forces are at the basis of Derjaguin-Landau-Verwey-Overbeek (DLVO) theory on colloid stability. That approach is modified when these forces, jointly operating in bio-based colloids, overlap with steric stabilization and depletion modes. Steric effects can be strongly dispersive even at high ionic strength, despite this is rather counterintuitive, when depletion ones favor the nucleation in a single phase.
Neurobehavioural Mechanism of Antidepressant Effect of Methanol Stem Bark Extract of Adansonia digitata (Linn) in Tail Suspension Test in Mice

Aim: An earlier study has demonstrated the in-vivo antidepressant effect of methanol stem bark extract of Adansonia digitata, using soxhlet extraction protocol, but there is a lack of scientific data on its neurobehavioural mechanism of action. This study, therefore, investigated its antidepressant potentials, using cold maceration method, and determined the probable neurobehavioural mechanism of its antidepressant-like effect. Methodology: The antidepressant-like effect of the extract was evaluated in tail suspension test, at graded doses in mice. Subsequently, the probable neurobehavioural mechanism of the antidepressant-like effect of the extract was investigated by intraperitoneal pretreatment with adrenergic, serotonergic, dopaminergic, and muscarinic cholinergic receptor antagonists; GABA agonist; nitric oxide precursor and inhibitors; and using a putative neuromodulator at NMDA receptors prior to the extract administration. Results and discussion: The extract at all the doses used, significantly (p 0.05) alteration on locomotor behaviour in mice. However, the anti-immobility potential of the extract was significantly (p<0.05) reversed by prazosin, yohimbine, sulpiride, methylene blue, L-arginine and baclofen, suggesting the involvement of adrenergic, dopaminergic, GABAerargic and nitergic pathways. Conclusion: This study, therefore, concluded that the extract may possess antidepressant effect and its mechanism may involve multiple pathways.
Size assessment in polychaete worms—application of morphometric correlations for common North Atlantic taxa

Body size is a basic animal feature that defines its functioning in multispecies assemblages. Polychaetes are numerically dominant components of marine macrobenthos, playing a key role in benthic productivity. They are also the most problematic group regarding body‐size assessments due to common fragmentation of fragile bodies during sample processing that inhibits direct assessments of their size and biomass. Here, we present quantified relationships that allow an estimation of the total‐body length based on morphometric features (widths of thoracic chaetigers) that remain intact after standard macrobenthic sample treatment. The best‐fitted regression equations (p < 0.001, r from 0.41 to 0.99) were selected for 28 polychaete families, six orders, two infraclasses, and one subclass based on the measurements on 3580 complete individuals collected in the North Atlantic (Norwegian and Spitsbergen fjords and continental margin). In Capitellidae, Cirratulidae, Oweniidae, and Trichobranchidae, the shapes of the relationships differed among dominant species/genera, and so, specific formula was proposed. The method has been applied to assess the size spectra of macrozoobenthos based on 18 van Veen grab samples collected at six stations in two Spitsbergen fjords. The percentage contribution of complete individuals in polychaete biomass and abundance in size classes varied between 0% and 43%, with the lowest percentages noted in two dominant families—Cirratulidae and Lumbrineridae. The presented method is likely to be widely applied in studies requiring polychaete individual size assessments (e.g., benthic biomass size spectra, population dynamics, and secondary production).
BIOSORPTION OF CADMIUM AND LEAD USING SUSPENDED AND IMMOBILIZED Enterobacter cloacae AT DIFFERENT PH

The aim of this work was to determine the best conditions for biomass production of the bacterium Enterobacter cloacae and for Cd and Pb retention by adsorption on the cell walls or by absorption and accumulation in the bacterial biomass. Suspended and immobilized cultures of this bacterium, acclimated to both metals, were grown for 24 h at pH 6, 7 and 9, in trypticase soy (TS) medium added with 2.73 mg/L of Cd or 16.8 mg/L of Pb. Immobilized cultures at pH 7 and 9 had the highest biomass production. Percentages of Cd retention by adsorption and absorption increased with increasing pH, but adsorption was higher in suspended while absorption was higher in immobilized cultures. Possibly because of different affinity of bacterial cells for these metals, the percentages of Pb retention were higher than those of Cd. In percentage, the highest Pb adsorption on cell walls was at pH 9, while retention by biomass decreased with increasing pH. The amount of metal retained by one g of bacterial biomass, efficiency was higher at pH 6, possibly because of the presence of dead or dying bacterial cells.
The Effects of Drinking Water Supplemented with Essential Oils on Performance, Egg Quality and Egg Yolk Fatty Acid Composition in Laying Hens

DOI: 10.26650/actavet.2018. 410397 This study was performed to examine the effect of different concentrations of an essential oil mixture (EOM) added to drinking water on growth performance, internal and external egg quality, and egg yolk fatty acid composition in laying hens. A total of 240 Brown Nick laying hens aged 20 weeks were used in this study. These hens were divided into one control and three treatment groups whose drinking water was supplemented with 0, 0.1, 0.2, and 0.3 ml/L of Oregofarm EOM. Oregofarm is a commercially available product containing peppermint, oregano, and anise oil. Each treatment group was further categorized into 10 subgroups, each consisting of 6 laying hens. The hens were fed basal rations for 16 weeks. For data collection and analysis, a 16-week period was divided into three time periods of 5 weeks. The weights of 25- to 30- and 35-week eggs were recorded weekly. In the groups whose drinking water was supplemented with the EOM, there was a significant improvement in egg production (EP) (p 0.05). The levels of saturated fatty acids were decreased, whereas the levels of polyunsaturated fatty acids increased with EOM supplementation, although the differences were not statistically significant (p>0.05). Therefore, based on the beneficial effects of supplementing drinking water with different concentrations of EOM on egg weight, egg production, feed conversion ratio, and egg yolk fatty acid compositions, it is suggested that EOM can be safely added at concentrations of up to 0.3 ml/L (i.e., the levels used in this study) to drinking water for laying hens.
Promising Antibacterial Effect of Copper Oxide Nanoparticles against Several Multidrug Resistant Uropathogens

Background: Recently, nanotechnology has been demonstrated to be a promising application to overcome the problem of antibiotic resistance. In the present study, we aimed to determine the antibacterial activity of copper oxide nanoparticles (CuO NPs) on several multiple-drug resistant (MDR) uropathogenic strains. Methods: This in vitro case-control study was performed on 4 uropathogenic bacteria including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. The antibacterial property was evaluated by well diffusion method at different concentrations of CuO NPs. Results: Overall, NPs concentration of 10, 25 and 50 µg/mL showed the remarkable antibacterial activity. A lower effect was seen against S. aureus strains. CuO NPs exhibited maximum bacterial growth inhibition against E. faecalis strains. In most of the cases, the zone of inhibition in 50 µg/mL concentration was closest to control positive antibiotics. Conclusion: In summary, CuO NPs as an alternative to conventional antibiotics that are currently used showed dose-dependent on antibacterial activity against different uropathogens, specificity towards pathogenic Gram-positive bacteria. This promising antibacterial activity of CuO NPs suggesting the development of NPs coatings on the different surface of biomedical materials for applications in different antimicrobial control systems.
Competence of Agrobacterium rhizogenes to accumulate heavy metals and toxicity of cadmium and copper to Agrobacterium strains

Heavy metals cannot be degraded through various treatments. Bioaccumulation and bioaugmentation of heavy metals by food chain could damage normal physiological activity of higher organisms and threat human life. Heavy metals are very toxic even at low concentration (1.0 – 10 μg/ l) and their toxicity can last for a long time in nature. Short-deep treatment beds constructed at Abo-Attwa, Experimental Station, Ismailia, Egypt were efficient in the removal of organic matter, BOD and COD from primary treated influent by 50%, 66 % and 68 %, respectively. Also, the treatment beds were efficient in the removal of 93 – 98 % of the heavy metals Cu, Pb, Zn, Ni and Cd from influent. Eight Agrobacterium strains, three A. rhizogenes and five A. tumefaciens, were isolated and characterized from naturally-occurring microbial flora proximate domestic wastewater of Abo-Attwa Experimental Station, Ismailia, Egypt. The Agrobacterium strains were able to accumulate lead, zinc and nickel at varying rates. On the other hand, copper and cadmium were toxic to all Agrobacterium strains tested. The toxicity order of various heavy metals to Agrobacterium strains tested was Cd> Cu> Zn> Ni> Pb. The three A. rhizogenes strains accumulated higher amounts of Zn and Ni than A. tumefaciens strains. Induction of some tolerant Agrobacterium strains via growth adaptation in the presence of progressive high concentration of Cd and Cu was achieved. A. rhizogenes strain A8 was superior in adaptation and survival as tolerant strain to both cadmium (Cd) and copper (Cu) on growing in Nutrient Broth containing elevated concentrations 1, 3, 10 and 30 μg/ l of both metals. The A. rhizogenes strains A1 and A7 efficiently accumulated 90 % more Cd than non-adapted Agrobacterium cells when grown at 10μg/l. Furthermore, The A. rhizogenes strains A1, A7 and A8 accumulating 90 % , 75 % and 80 % respectively more Cu than non-adapted Agrobacterium cells when grown at 10μg/l. The bioaccumulation efficiency % of the A. rhizogenes strains A1 and A7 was 40 % for accumulating 30μg/l of the toxic metal cadmium (Cd). The potential role of Agrobacterium in the bioremediation of heavy metals was also considered.
Chemical composition of leaves, inflorescence, whole aerial-parts and root essential oils of patchouli {Pogostemon cablin (Blanco) Benth.}

ABSTRACT The essential oils extracted from the leaves, inflorescence, whole aerial-parts and root of patchouli [Pogostemon cablin (Blanco) Benth. ] were analyzed and compared using GC-FID and GC-MS. Altogether thirty-four constituents, representing 86.9–97.7% of the total oil compositions, were identified. Major constituents of the oils of aerial-parts (leaves, inflorescence and whole aerial-parts) were patchouli alcohol (42.2–57.7%), α-bulnesene (9.0–15.2%), α-guaiene (6.4–17.9%), seychellene (3.4–6.9%), pogostol (0.3–5.0%) and (E)-caryophyllene (2.1–3.6%). However, the root oil was characterized by higher amount of pogostone (70.2%), norpatchoulenol (5.3%) and β-pinene (4.5%).