Miracle-dz/UniFAR-FTUs huggingface dataset:
In Vitro Processing of Recombinant G Protein y Subunits REQUIREMENTS FOR ASSEMBLY OF AN ACTIVE /3y COMPLEX*

The y subunits of the heterotrimeric G proteins (G,) are subject to carboxyl-terminal processing. This processing involves prenylation of a cysteine residue ini- tially 4 amino acids from the carboxyl terminus, endoproteolytic truncation of the 3 terminal amino acids, and methylation of the now carboxyl-terminal prenylcysteine residue. The significance of each of these modi- fications in the ultimate properties of G proteins is not yet clear. We have developed in vitro systems for the production of the three processing intermediates (un-modified, prenylated, and truncated-prenylated) for two G, subunits, one which is subject to farnesylation (GY1) and one which is geranylgeranylated (G,,& Assessment of the functional consequences of the processing of G, was found to require reconstitution of the polypeptides with a G protein p subunit (G,). The ability of recombi- nant G,, produced in Sf9 cells, to assemble into stable Py complexes (G,,,) with each of the G, processing intermediates was assessed. Both prenylated and unprenylated G, subunits formed stable complexes with G,, but sur-prisingly, neither of the truncated-prenylated G, sub- units were competent for this assembly. The G,, complexes which were formed were examined for their ability to interact with a G protein a subunit (Gm). Only those G,, complexes containing a prenylated G, subunit were functional in this were preincubated with 4 pg of the resolved rGel fraction (-30-50 as estimated by immunoblot) at 30 "C 30 50 mM Tris-HC1, pH 8.0, 1 mM DTT, 100 mM NaCI, 0.1 mM EDTA, and 0.01% Lubrol. After the assembly reaction, the ADP-ribosylation reaction was initiated by diluting the preincubated rG,,, and rG, mixture 4-fold by addition of the ADP-ribosylation reaction described above. were incubated for 30 "C and and as Electrophoresis and Immunoblotting-The G, and G, proteins were resolved on 12 and 16% polyacrylamide-SDS gels, respectively. SDS-polyacrylamide gel electrophoresis was carried out as described (22), except that the G, protein gels were electrophoresed in 25 mM Tris-HCI, pH 8.3, 0.2 M Tricine, and 0.1% SDS. Gels were calibrated with low molecular weight markers for stained gels or prestained markers for immunoblot analysis (both from Bio-Rad). To visualize "-labeled samples, gels were prepared for fluorography by incubation in 1 M salicylic acid for 30 min prior to drying the gels then exposing to RX x-ray film (Fuji). Immunoblot analysis was performed using antiserum 985, a polyclonal rabbit antibody directed against a peptide spanning residues 127-139 of the G rotein. The antiserum was obtained from Dr. Thomas Gettys (this Institution). Primary antibody binding was detected using goat anti-rabbit IgG alkaline phosphatase conjugate (Promega). chloromethyl
Understanding the open circuit voltage in organic solar cells on the basis of a donor-acceptor abrupt (p-n++) heterojunction

By using electrical characterization and classical solid state semiconductor device theory, we demonstrate that the open circuit voltage ( V oc ) in organic solar cells based on non-intentional doped semiconductors is fundamentally limited by the built-in potential ( V bi ) originated at a donor-acceptor abrupt (p-n ++ ) heterojunction in case of selective contacts. Our analysis is validated using P3HT:PCBM devices fabricated in our research group. We also demonstrate that such a result can be generalized using data already reported in literature for fullerene- based solar cells. Finally, we show that the dependence of V oc on the device contacts can be understood in terms of the potential barriers formed by the Fermi level alignment of semiconductors at the heterojunction and at the Schottky junctions.
An Algorithm of Vocabulary Enhanced FLAT 1

The main purpose of the intelligent question answering system based on the knowledge graph is to accurately match the natural language question and the triple information in the knowledge graph. Among them, the entity recognition part isoneofthekeypoints.Thewrongentityrecognitionresultwillcausetheerrortobe donepropagated,resultingintheultimatefailuretogetthecorrectanswer.Inrecentyears,thelexicalenhancementstructureofwordnodescombinedwithwordnodes hasbeenprovedtobeaneﬀectivemethodforChinesenamedentityrecognition. Inordertosolvetheaboveproblems,thispaperproposesavocabulary-enhancedentity recognitionalgorithm(KGFLAT)basedonFLATforintelligentquestionansweringsystem.Thismethodusesanewdictionarythatcombinestheentityinformationof theknowledgegraph,andonlyuseslayernormalizationfortheremovalofresidualconnectionfortheshallowernetworkmodel.Thesystemusesdataprovidedby theNLPCC2018Task7KBQAtaskforevaluation. Theexperimentalresultsshowthatthismethodcaneﬀectivelysolvetheentityrecognitiontaskintheintelligent questionansweringsystemandachievetheimprovementoftheFLATmodel,andtheaverageF1valueis94.72
Passive control of wave propagation in periodic anti-tetrachiral meta-materials

Periodic anti-tetrachiral materials are strongly characterized by a marked auxeticity, the unusual and fascinating mechanical property mathematically expressed by negative values of the Poisson’s ratio. The auxetic behavior is primarily provided by pervasive rolling-up mechanisms developed by the doubly-symmetric micro-structure of the periodic cell, composed by a regular pattern of rigid rings connected by tangent flexible ligaments. Adopting a beam-lattice model to describe the linear free dynamics of the elementary cell, the planar wave propagation along the bi-dimensional material domain can be studied according to the Floquet-Bloch theory. Parametric analyses of the dispersion curves, carried out with numerical or asymptotic tools, typically reveal a highly-dense spectrum, with persistent absence of total band-gaps in the low-frequency range. The paper analyses the wave propagation in the meta-material developed by introducing rigid massive inserts, locally housed by all the rings and working as undamped linear oscillators with assigned inertia and/or stiffness properties. The elastic coupling between the cell microstructure and the oscillators, if properly tuned (inertial resonators), is found to significantly modify the Floquet-Bloch spectrum of the material. The effects of the resonator parameters (tuning frequency and mass ratio) on the low-frequency band structure of the metamaterial are discussed, with focus on the valuable possibility to (i) open total band gaps, by either the widening of an existing partial band gap or the avoidance of a crossing point between adjacent dispersion curves, (ii) finely control the total band-gap amplification, in order to assess the maximum achievable performance of the meta-material against the vibration propagation
Improved ptychographic inspection of EUV reticles via inclusion of prior information

: The development of actinic mask metrology tools represents one of the major challenges to be addressed on the roadmap of extreme ultra violet (EUV) lithography. Technological advancements in EUV lithography result in the possibility to print increasingly ﬁne and highly resolved structures on a silicon wafer, however the presence of ﬁne–scale defects, interspersed in the printable mask layout, may lead to defective wafer prints. Hence the development of actinic methods for review of potential defect sites becomes paramount. Here, we report on a ptychographic algorithm that makes use of prior information about the object to be retrieved, generated by means of rigorous computations, to improve the detectability of defects whose dimensions are of the order of the wavelength. The comprehensive study demonstrates that the inclusion of prior information as a regularizer in the ptychographic optimization problem results in a higher reconstruction quality and an improved robustness to noise with respect to the standard ptychographic iterative engine (PIE). We show that the proposed method decreases the number of scan positions necessary to retrieve an high quality image and relaxes requirements in terms of signal to noise ratio (SNR). The results are further compared with the state–of–art total variation based ptychographic imaging.
WRA Ż LIWO ŚĆ NA OLEJKI ETERYCZNE Ś RODOWISKOWYCH LEKOOPORNYCH SZCZEPÓW Escherichia coli SENSITIVITY TO THE ESSENTIAL OILS OF ENVIRONMENTAL, RESISTANT TO DRUGS STRAINS OF Escherichia coli

: In an environment increasingly common finding is the presence of antibiotic-resistant strains of Escherichia coli . An alternative way to eliminate them can provide biodegradable and non-toxic substances, including essential oils. The aim of this study was to evaluate the effect of essential oils on environmental, antibiotic-resistant strains of Escherichia coli . The research material consisted of 10 isolated from the environment, antibiotic-resistant strains of E. coli , including 8 glucuronidase-positive. Using the Mueller-Hinton medium was evaluated the sensitivity to antibiotics such as ampicillin, amoxicillin, cefotaxime, chloramphenicol, ciprofloxacin, ceftazidime, doxycycline, gentamycin, kanamycin, trimethoprim, tetracycline, streptomycin, nalidixic acid and sulfonamides. Using the platelet-cylinders diffusion method was assessed the sensitivity of the strains on essential oils: thyme, oregano, tea tree, lemongrass, lemon, and caraway, at concentrations of 1.5%; 1.0%; 0.5%, 0.25%. Based on the results, it was found that the tested strains of E. coli were resistant to at least half of the used antibiotics and 90% of them showed resistance to ampicillin, and amoxicillin. Also, the sensitivity of the strains to tested essential oils has been diverse. Only thyme oil in a concentration of 1.5% inhibited growth of 90% of the tested strains of E. coli . On the other hand, oregano oil in a concentration of 1.5% inhibited the development of 3 out of 10 tested strains. Oils of tea, lemongrass, lemon and caraway, at the mentioned concentrations have been not bactericidal against strains of Escherichia coli .
Psicologia: Teoria e Pesquisa

This article aimed to analyze hearing voices experiences in patients of a Psychosocial Care Center. In this regard, ten people were interviewed. The qualitative method was used, with content analysis. Four categories were chosen: “origin” of voices, phenomenology of voices, coping strategies, and family support. Hallucinatory experiences have emerged in contexts of violence and isolation. Topographic variables of the voices indicate possibilities of understanding the phenomenon. The movement is a basic condition to deal with the experiences. Family support is key to cope with difficulties. It is necessary to qualify the auditory hallucination as a meaningful experience, which must be respected in any intervention that intends care.
PENGARUH PERBEDAAN INTENSITAS CAHAYA D AN PENYIRAMAN PADA PERTUMBUHAN JAGUNG ( ZEA MAYS L.) ‘SWEET BOY -02’ THE EFFECT OF DIFFERENT LIGHT INTENSITY AND WATER TREATMENT TO THE GROWTH OF MAIZE ( ZEA MAYS L.) ‘SWEET BOY

The light intensity and water availability are the factors both supporting and supressing plant growth and it depends on which level that plant can accept. The aim of this research were to evaluate the effect of light intensity and watering in maize ‘Sweet Boy-02’ growth and to predict the suitable condition for maize productivity. This research used Randomized Completed Design with 3 regimes of light intensity (L1 : 63694, L2 : 11408 dan L3 : 3897 Lux) and 3 regimes of watering (W1 : 2 L, W2 : 1,6 L and W3 : 1,2 L). Each combination was done with 3 replications and it was done in Sawitsari, Yogyakarta. Maize was harvested in 75 days after the treatment and the measured parameters were plant height, leaf total number, leaf’s widthlenght ratio, plant biomass, and root-shoot ratio. Data were analyzed by Anava and DMRT test with significance level of 5%. The results showed that L1W1 treatment caused plant height reached 188 cm, leaf total number was 10, and leaf’s width-lenght ratio was 0,06. Meanwhile, plant biomass was 51,34 gr and root-shoot ratio was 0,097.
Legislative Communication and Power: Measuring Leadership in the U.S. House of Representatives from Social Media Data

Who leads and who follows in Congress? By leveraging the Twitter accounts of U.S. House of Representatives members, we develop a new understanding of House leadership power using natural language processing methods in new ways. Formal theoretic work on congressional leadership suggests a tension in legislative party members’ policy stances as they balance coordination and information problems. When their coordination problem is more pressing, the model predicts that legislative members will follow their party leaders’ policy positions. When the information problem is more acute, party members coordinate and give their leaders direction for the party’s agenda. We introduce novel ways to measure dynamic policy inﬂuence that then enable testing of these hypotheses. Speciﬁcally, we exploit the network structure of retweets to derive measures of House leadership centrality within each party. We also use Joint Sentiment Topic modeling to quantify the discussion space for House members on Twitter. Our results provide support for the theoretical insights.
Effects of boron penetration and resultant limitations in ultra thin pure-oxide and nitrided-oxide gate-films

The boron penetration effect was compared for p/sup +/ poly gate PMOSFETs with pure oxide gates and nitrided oxide gates. For a gate thickness of 6.5 nm, reduced boron dosage and rapid thermal processing solve the problem of boron penetration in the pure oxide case. However, when the film thickness is less than 6.5 nm, only a nitrided oxide film can solve the problem. From the results of EDX analysis in nitrided oxide films, it was found that nitrogen build-up at the interface is small and that a nitrogen concentration of only a few percent leads to complete suppression of boron penetration down to the 2 nm range of film thickness. Excellent characteristics in 2.6 nm nitrided oxide gate p-MOSFETs, free from boron penetration effects, were demonstrated. <<ETX>>
Determination of th Compensation Poin

A method is described for the pensation point (ACP) of plant tissue, i.e duction is minimum. The rate of CO2 prod an exponentially declining O2 concentration bottle initially containing air. Too rapid a because of the time required for the tissue The ACP is easily determined from a plo Rates of CO2 production and ACPS calculat are similar to those calculated from traditi under various O2 concentrations. Interest in the use of thin plastic films to produce modified atmospheres in packages (Lakin, 1987; Zagory and Kader, 1988) and in the use of low-O2 atmospheres as quarantine treatments for the control of insects is high (Gaunce et al., 1982; Klag, 1985; Lidster et al., 1981; Michell et al., 1984; Soderstrom and Brandl, 1985). Successful application of these developing technologies requires detailed knowledge of the relation between the rate of respiration by the plant tissue and the gaseous atmosphere surrounding it. Of special interest is the anaerobic compensation point (ACP), which is defined as the O2 concentration at which CO2 production is minimum (Boersig et al., 1988). The ACP could indicate the lower limit for the O2 content of storage atmospheres for numerous commodities grown and stored under a wide range of conditions. The postharvest literature contains various studies in which the ACP has been determined for some commodities (Blackman, 1928; Boersig et al., 1988; Thomas and Fidler, 1933). The traditional method of determining the ACP involves measuring the rate of CO2 production at several O2 concentrations. However, this method is time-consuming and often only approximates the true ACP, since it must be calculated from respiration measurements taken at discrete O2 concentrations. In this paper, we describe a simple, rapid method for determining the ACP in which the O2 concentrations of the atmosphere surrounding the tissue is reduced from 20.9% to near 0%. This method eliminates the need for syringe-sampling of CO2 concentrations and for preparing numerous gas mixtures, since continuous readings of Received for publication 8 June 1989. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.
Analytical modelling and device design optimisation of epitaxial layerâ•’based IIIâ•fiV tunnel FET

The line tunnelling and heterojunction are two important techniques to improve the performance of the tunnelling field-effect transistors (TFETs). The TFETs that utilise both of these techniques perform superior to the conventional TFETs. The recently proposed T-shaped TFET (TTFET) is one such heterojunction-based line tunnelling device that is expected to become energy efficient switch. For the first time, a physics-based analytical model for surface potential and drain current of epitaxial layer-based heterojunction line TFET has been developed. The model describes the impact of device design parameters on the electrical performance of the device. The Poisson equation is solved with precise boundary conditions to obtain the surface potential model. Kane's model is used to calculate drain current by utilising surface potential model. A good agreement between Synopsys technology computer-aided design simulation and analytical model is observed with 5.4% error in ON current at VGS = VDS = 0.5 V, an average error of 5.80% in surface potential and 7.24% in transconductance. Finally, a device design guideline is presented according to the analytical expressions.
Agricultural Robot Can Be Controlled and Monitored Through the LABVIEW

This paper explains the microcontroller based fuzzy controller for an agricultural robot which can be used to plough the field, seeding and soil moisture sensing. By using the agricultural robots in field which can be used to reduce manpower and can be operated using remote controls from a distant place. In this paper, an agricultural robot is designed with an internet based remote control by using LAB VIEW and its position and speed control are discussed. Fuzzy controller is designed to change the steering angle and the speed of the robot according to the desired reference position. The control was implemented using Atmega16 Microcontroller and the results are documented. The Robot control is implemented from a remote place using internet and web publishing tool in LabVIEW. Thus this agricultural Robot control reduces the manpower and becomes advantageous and cost-effective. In existing they are using RF technology for transmitting and receiving purposes. But RF module operates at 433.92 MHZ. and it can transmits the information about a single robot. This is is one of the main disadvantages in existing system. so only GPRS technology is proposed to overcome this problem. By using the GPRS transmission of data is very fast,long distance communication and transmits the information about multiple number of robots.
C ognitive flexibility among female migraine patients: case–control study

Öz Purpose: The aim of this study was to determine if cognition is affected in female migraine sufferers by comparing cognitive domains with a healthy control group. Material s and Methods: Fifty patients with migraine and 50 healthy controls (all female) were enrolled in this cross sectional case – control study. The Beck Depression test, Beck Anxiety test, California Verbal Learning Test, Montreal Cognitive Assessment Scale, and Wisconsin Card Sorting Test (WCST) were performed in both groups. Results: Using a multivariate logistic regression model of migraine, WCST non - perseverative error (odds ratio [OR], 1.62; 95% confidence interval [CI], 1.028 –2.568; p = 0.03), WCST percent of perseverative error (OR, 0.23; 95%CI, 0.071–0.786; p = 0.01), WCST perseverative response (OR, 4.55; 95%CI, 1.272 –16.298; p = 0.02), no alcohol consumption (OR, 0.03), WKET perseveratif hata yüzdesi (OR, 0.23; 95%CI, 0.071–0.786; p = 0.01), WKET perseveratif tepki (OR, 4.55; 95%CI, 1.272 –16.298; p = 0.02), alkol kullanmama (OR, 0.006; 95%CI, 0.000 – 0.943; p = 0.04), ailede hipertansiyon hikayesi (OR, 4.46; 95%CI, 1.114 –17.915; p = 0.03), ailede migren hikayesi (OR, 4.028; 95%CI, 1.027– 15.799; p = 0.04), and ailede inme hikayesi olmaması (OR, 0.034; 95%CI, 0.003 –0.448; p = 0.01) migreni oluşturan multivaryat lojistik regresyon modelinde yer aldı. WKET skorlarından perseveratif olmayan kişinin problem çözme becerisini gösterir. Perseveratif hata yüzdesi ve perseveratif tepki ise kişinin bilişsel değişken ettirdiği beceriyi
Barriers and opportunities of combining social and ecological functions of urban greenspaces – Users’ and landscape professionals’ perspectives

Combined provision of social and ecological functions of urban green spaces (UGSs) can facilitate sustainable development by combating the adverse effects of rapid urban growth. However, barriers and opportunities of such provision need to be better understood so that policy-makers and managers can ensure their benefits are realised. This paper addresses this by investigating user and manager preferences for different types, characteristics, facilities and activities associated with UGSs, and by exploring academic and managerial perceptions about policy and management practices. Empirical evidence from two UK cities – Manchester and Leeds – revealed that the barriers and opportunities to combining social and ecological functions in UGSs were linked to user demand; knowledge and understanding about UGS functions; and budget/funding. The research also indicated that addressing these issues would require the active promotion of a number of key priority areas. These included enhancing awareness and knowledge amongst both users and managers, the development of management guidelines linked to combining functions, and better use of context specific measures grounded in innovative techniques.
ASTRONAUT BIO-SUIT SYSTEM TO ENABLE PLANETARY EXPLORATION

The Bio-Suit System concept is designed to revolutionize human space exploration by providing enhanced astronaut extravehicular activity (EVA) locomotion and performance, based on the idea of a ‘second skin’ capability and of biomechanically and cybernetically augmented human performance capacity. The novel Bio-Suit concept provides an overall exploration system realized through symbiotic relationships between a suite of advanced technologies, creative design, and human modeling and analysis. By working at the intersection of engineering, design, and operations, new emergent capabilities and interrelationships could help realize the new Vision for Space Exploration. The second skin envisioned is capable of augmenting our biological skin by providing mechanical counter pressure. Perhaps the ‘epidermis’ of such a second skin will be a ‘spray-on’ polymer providing natural locomotion for explorers. Several concepts for a mechanical counter pressure suit have been developed, and subsequently, initial prototypes have been tested. To determine engineering requirements for future prototypes, the three-dimensional anthropometry of the moving human body has been studied.
Relationship Between Peer Counseling Activities and Pupils’ Discipline in Public Primary Schools in Mathioya Sub-county, Murang’a County, Kenya

Cases of disruptive behavior problems such as inattentiveness, bullying, violence, drug abuse, hitting other pupils, teasing others, yelling and complaining amongst pupils in public primary schools in Mathioya Sub-county are on the rise. The purpose of this study was to evaluate the relationship between peer counseling activities and pupils’ discipline in public primary schools. A sample size of 196 respondents were selected. The study established that there was significant relationship between peer counsel ing activities and pupils’ discipline at the p<0.05[r = 0.001, p = 0.001]. This indicates that, in public primary schools where teachers frequently engage in peer counseling activities, pupils manifest few cases of indiscipline. The implications of this finding are discussed.