Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) of Bacillus subtilis BS-1 significantly inhibits the mycelial growth of the pathogen Botryosphaeria dothidea and attenuates the pathogenicity on kiwifruit in a concentration-dependent manner. In particular, mycelial growth diameter was only 21% of the control after 3 days of treatment with 5% CFS. CFS caused swelling and breakage of the hyphae of B. dothidea observed by scanning electron microscopy, resulting in the leakage of nucleic acid and soluble protein and the loss of ergosterol content. Further analysis demonstrated that CFS significantly induces the expression of Nox genes associated with reactive oxygen species (ROS) production by 1.9–2.7-fold, leading to a considerable accumulation of ROS in cells and causing mycelial cell death. Our findings demonstrate that the biocontrol effect of B. subtilis BS-1 CFS on B. dothidea is realized by inducing oxidative damage to the mycelia cell.
Mechanical Stretch Activates TRPV4 and Hemichannel Responses in the Nonpigmented Ciliary Epithelium

Previously, we reported a mechanosensitive ion channel, TRPV4, along with functional connexin hemichannels on the basolateral surface of the ocular nonpigmented ciliary epithelium (NPE). In the lens, TRPV4-mediated hemichannel opening is part of a feedback loop that senses and respond to swelling. The present study was undertaken to test the hypothesis that TRPV4 and hemichannels in the NPE respond to a mechanical stimulus. Porcine NPE cells were cultured on flexible membranes to study effects of cyclic stretch and ATP release was determined by a luciferase assay. The uptake of propidium iodide (PI) was measured as an indicator of hemichannel opening. NPE cells subjected to cyclic stretch for 1–10 min (10%, 0.5 Hz) displayed a significant increase in ATP release into the bathing medium. In studies where PI was added to the bathing medium, the same stretch stimulus increased cell PI uptake. The ATP release and PI uptake responses to stretch both were prevented by a TRPV4 antagonist, HC067047 (10 µM), and a connexin mimetic peptide, Gap 27 (200µm). In the absence of a stretch stimulus, qualitatively similar ATP release and PI uptake responses were observed in cells exposed to the TRPV4 agonist GSK1016790A (10 nM), and Gap 27 prevented the responses. Cells subjected to an osmotic swelling stimulus (hypoosmotic medium: 200 mOsm) also displayed a significant increase in ATP release and PI uptake and the responses were abolished by TRPV4 inhibition. The findings point to TRPV4-dependent connexin hemichannel opening in response to mechanical stimulus. The TRPV4-hemichannel mechanism may act as a mechanosensor that facilitates the release of ATP and possibly other autocrine or paracrine signaling molecules that influence fluid (aqueous humor) secretion by the NPE.
Critical re‐evaluation of the bulk transfer coefficient for sensible heat over the ocean during unstable and neutral conditions

A new analysis of the neutral heat transfer coefficient CHN on data from Östergarnsholm is presented, which is primarily based on a limited set of measurements with the very accurate MIUU (Meteorological Institute of the University of Uppsala) instrument, but with additional information from an extensive set of measurements with Solent sonic R2. Sonic data are, however, used with great caution, since for wind speed U above 10 m s−1, a strongly wind‐speed‐dependent correction is shown to be required. This error is roughly proportional to (U − 10) for sea–air temperature differences less than 4–5 K. For a larger temperature difference, no correction appears to be necessary in the wind speed range 10–15 m s−1.
A New CPW-Fed Semicircular Inverted Triangular Shaped Antenna Based on Mixed-Alternate Approach for 5G Millimeter-Wave Wireless Applications

This paper presents the design and development of a new semicircular inverted triangular shaped antenna for 5G millimeter-wave wireless applications. An alternate-mixed approach based on cavity, slots and loaded stubs is employed in the designed antenna lattice. The suggested antenna structure is formed by a radiator, partial defected metal ground plane and a 50 Ω coplanar waveguide. The proposed antenna resonated at multiple frequencies by the setting up of the proper dimensions and locations of the rectangles, elliptical cut slots and cavity stubs. Furthermore, a parametric analysis is carried out to examine the antenna’s effectiveness and impedance-matching controls. The proposed structure is realized on the low-cost RT/Duroid Rogers RO3010™ laminate with an overall small size of 1. 381λ0 × 1. 08λ0 × 0. 098λ0, where λ0 represents the wavelength corresponding to the minimum edge frequency of the 23 GHz at 10 dB impedance bandwidth of the antenna. The antenna’s key characteristics in terms of bandwidth, gain, radiation patterns and current distribution have been investigated. The antenna exhibits high performance, including an impedance bandwidth of 19 GHz ranging from 23 GHz to 42 GHz, results in 58.46% wider relative bandwidth calculated at 10 dB scaled return loss, a peak realized gain of 6.75 dBi, optimal radiation efficiency of 89%, stable omnidirectional-shaped radiation patterns and robust current distribution across the antenna structure at multiple resonances. The designed antenna has been fabricated and simulation experiments evaluated its performance. The results demonstrate that the antenna is appropriate and can be well integrated into 5G millimeter-wave wireless communication systems.
Platinum Nanoparticles Immobilized on Electrospun Membranes for Catalytic Oxidation of Volatile Organic Compounds

Structured catalytic membranes with high porosity and a low pressure drop are particularly suitable for industrial processes carried out at high space velocities. One of these processes is the catalytic total oxidation of volatile organic compounds, which is an economically feasible and environmentally friendly way of emission abatement. Noble metal catalysts are typically preferred due to high activity and stability. In this paper, the preparation of a thermally stable polybenzimidazole electrospun membrane, which can be used as a support for a platinum catalyst applicable in the total oxidation of volatile organic compounds, is reported for the first time. In contrast to commercial pelletized catalysts, high porosity of the membrane allowed for easy accessibility of the platinum active sites to the reactants and the catalytic bed exhibited a low pressure drop. We have shown that the preparation conditions can be tuned in order to obtain catalysts with a desired platinum particle size. In the gas-phase oxidation of ethanol, acetone, and toluene, the catalysts with Pt particle sizes 2.1 nm and 26 nm exhibited a lower catalytic activity than that with a Pt particle size of 12 nm. Catalysts with a Pt particle size of 2.1 nm and 12 nm were prepared by equilibrium adsorption, and the higher catalytic activity of the latter catalyst was ascribed to more reactive adsorbed oxygen species on larger Pt nanoparticles. On the other hand, the catalyst with a Pt particle size of 26 nm was prepared by a solvent evaporation method and contained less active polycrystalline platinum. Last but not least, the catalyst containing only 0.08 wt. % of platinum achieved high conversion (90%) of all the model volatile organic compounds at moderate temperatures (lower than 335 °C), which is important for reducing the costs of the abatement technology.
Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity

Upper tract urothelial cancer (UTUC) is a less common disease in Western countries but has a high level of prevalence in Asian populations. Compared to bladder cancer, unique etiologic and genomic factors are involved in UTUC. Fibroblast growth factor receptor 3 (FGFR3) up-regulation has been proposed as a promising target for bladder cancer therapy. In this study, we aimed to profile the expression of FGFR3 in Asian and Caucasian UTUC tissues and to evaluate the in vitro therapeutic efficacy of small interference RNA (siRNA)-mediated FGFR3 silencing in UTUC treatment. The FGFR3 expression levels in renal pelvis tissues and microarray sections from Asian and Caucasian patients with UTUC, respectively, were measured via immunohistochemistry. The BFTC-909 and UM-UC-14 UTUC cell lines were used to examine the effects of FGFR3 silencing on proliferation, migration, epithelial–mesenchymal transition (EMT) marker expression, and signaling machinery. FGFR3 expression increased as the TNM stage increased in both Asian and Caucasian UTUC tumors, and no statistical difference was identified between the two groups. In vitro studies demonstrated that FGFR3 siRNA delivery significantly inhibited proliferation and migration and suppressed the expression of EMT markers and transcription factors in UTUC cells. Mechanistically, FGFR3 silencing alleviated the constitutive expression of RAS and the phosphorylation of MAPK signaling mediators, including ERK1/2 and JNK1/2. FGFR3 silencing elicited an apoptosis-inducing effect similar to that of FGFR inhibition. Conclusion: siRNA-targeted FGFR3 expression may impede the expansion and invasion of UTUC cells by alleviating the RAS/MAPK signaling pathway. The genetic interference of FGFR3 expression via siRNA in UTUC cells may constitute a useful therapeutic strategy.
Bacterial Cellulose—Carboxymethylcellulose Composite Loaded with Turmeric Extract for Antimicrobial Wound Dressing Applications

Bacterial cellulose (BC) is a biopolymer whose properties have been intensively studied, especially for biomedical applications. Since BC has no antimicrobial activity, it is necessary to use bioactive substances for developing wound healing applications. Another drawback of BC is the loss if its water retention capacity after dehydration. In order to overcome these problems, carboxymethyl cellulose (CMC) and turmeric extract (TE) were selected for the preparation of BC composites. Citric acid (CA) was used as the crosslinking agent. These composites were tested as potential antimicrobial wound dressing materials. TE-loaded BC–CMC composites were characterized in terms of their morphology, crystallinity, and thermal behavior. Swelling tests and curcumin-release kinetic analysis were also performed. All the composites tested had high swelling degrees, which is an advantage for the exudate adsorption from chronic wounds. The antibacterial potential of such composites was tested against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Candida albicans (C. albicans). The in vitro cytotoxicity toward L929 fibroblast cells was studied as well. The obtained results allow us to recommend these composites as good candidates for wound dressing applications.
Selective Eradication of Colon Cancer Cells Harboring PI3K and/or MAPK Pathway Mutations in 3D Culture by Combined PI3K/AKT/mTOR Pathway and MEK Inhibition

Colorectal cancer (CRC) is the second deadliest cancer in the world. Besides APC and p53 alterations, the PI3K/AKT/MTOR and MAPK pathway are most commonly mutated in CRC. So far, no treatment options targeting these pathways are available in routine clinics for CRC patients. We systematically analyzed the response of CRC cells to the combination of small molecular inhibitors targeting the PI3K and MAPK pathways. We used CRC cells in 2D, 3D spheroid, collagen gel cultures and freshly isolated organoids for drug response studies. Readout for drug response was spheroid or organoid growth, spheroid outgrowth, metabolic activity, Western blotting and immunofluorescence. We found profound tumor cell destruction under treatment with a combination of Torin 1 (inhibiting mTOR), MK2206 (targeting AKT) and selumetinib (inhibiting MEK) in 3D but not in 2D. Induction of cell death was due to apoptosis. Western blot analysis revealed efficient drug action. Gedatolisib, a dual PI3K/mTOR inhibitor, could replace Torin1/MK2206 with similar efficiency. The presence of PI3K and/or RAS-RAF-MAPK pathway mutations accounted for treatment responsiveness. Here, we identified a novel, efficient therapy, which induced proliferation stop and tumor cell destruction in vitro based on the genetic background. These preclinical findings show promise to further test this combi-treatment in vivo in mice and to potentially develop a mutation specific targeted therapy for CRC patients.
Summation Theorems for Hypergeometric Series in $U(n)$

Explicit expressions for the matrix elements of multiplicity-free Wigner and Racah coefficients in $U(n)$ are used to establish multidimensional analogs of known hypergeometric summation theorems. Infinite sequences of such analogs are found for the Gauss theorem, Saalschutz’ theorem, and the summation theorem for well-poised ${}_5 F_4 (1)$. For the sake of completeness, results published earlier are also presented: an infinite sequence of summation theorems analogous to that for well-poised ${}_4 F_3 (-1)$ and a single analog to Whipple's theorem which arises from the construction of tensor operators in $U(3)$.
An introduction to mathematical risk theory

Deviance sociology Wikipedia April 22nd, 2019 In sociology deviance describes an action or behavior that violates social norms including a formally enacted rule e g crime as well as informal violations of social norms e g rejecting folkways and mores Although deviance may have a negative connotation the violation of social norms is not always a negative action positive deviation exists in some situations
A note on calculating autocovariances of long‐memory processes

In this paper, we consider a method (splitting) for calculating the auto‐ covariances of fractional integrated processes (ARFIMA) and generalized integrated processes (GARMA). The splitting method does not require any restriction on the autoregressive roots, and allows fast calculation of the autocovariances of these processes.
SIE and OME as tools to estimate the uncertainty in the correction for the effect of impurities applied to metal–carbon eutectic systems

For high-purity metal fixed points the sum of individual estimates (SIE) and the overall maximum estimate (OME) have been introduced as tools to estimate the uncertainty in the correction for the effect of impurities. In this paper we investigate the feasibility of applying these tools to metal–carbon (M–C) eutectics as well on the basis of material collected for the eutectics Fe–C and Co–C. This would be of interest since it would obviate the need for modelling the full freezing or melting curve, which requires detailed knowledge of the material properties involved. For SIE we need only the liquidus slopes pertinent to the detected impurities, whereas for OME under certain conditions detailed knowledge of the impurity parameters would not be needed at all.
Characteristics of Aflatoxin B1 Degradation by Stenotrophomonas acidaminiphila and It’s Combination with Black Soldier Fly Larvae

Simple Summary Aflatoxin B1 (AFB1), one of the most hazardous mycotoxins commonly present in food and feed, causes great loss in livestock farming and severe safety risks to human health. In this paper, benefiting from using a sterile black soldier fly larvae (BSFL) system, we found that the ability of BSFL to degrade AFB1 was greatly reduced in the absence of gut microbiota, which indicated the important role of gut microbiota in AFB1 degradation. Furthermore, twenty-five AFB1-degrading bacteria were isolated from BSFL gut, and S. acidaminiphila A2 achieved the highest AFB1 degradation, by 94%. When S. acidaminiphila A2 was re-inoculated to BSFL, the detrimental effect of AFB1 on the growth performance of BSFL was alleviated, and complete AFB1 degradation in peanut meal was obtained. The present study may provide a strategy to degrade AFB1 in feedstuff through bioconversion with BSFL in combination with gut-originated AFB1-degrading bacteria, while providing a sustainable insect protein and fat source to animals. Abstract Aflatoxin B1 (AFB1) is a common mycotoxin contaminant in cereals that causes severe economic losses and serious risks to the health of humans and animals. In this paper, we investigated the characteristics of AFB1 degradation by black soldier fly larvae (BSFL) combined with commensal intestinal microorganisms. Germ-free BSFL and non-sterile BSFL were reared on peanut meal spiked with AFB1 for 10 days. The result showed that germ-free BSFL and non-sterile BSFL could achieve 31.71% and 88.72% AFB1 degradation, respectively, which indicated the important role of larvae gut microbiota in AFB1 degradation. Furthermore, twenty-five AFB1-degrading bacteria were isolated from BSFL gut, and S. acidaminiphila A2 achieved the highest AFB1 degradation, by 94%. When S. acidaminiphila A2 was re-inoculated to BSFL, the detrimental effect of AFB1 on the growth performance of BSFL was alleviated, and complete AFB1 degradation in peanut meal was obtained. In conclusion, the present study may provide a strategy to degrade AFB1 in feedstuff through bioconversion with BSFL in combination with gut-originated AFB1-degrading bacteria, while providing a sustainable insect protein and fat source to animals.
The Influence of Long-Time Storage on the Structure and Properties of Multi-Block Thermoplastic Polyurethanes Based on Poly(butylene adipate) Diol and Polycaprolactone Diol

A series of semi-crystalline multi-block thermoplastic polyurethanes (TPU), containing poly(butylene adipate) (PBA), polycaprolactone (PCL) and their equimolar mixture (PBA/PCL) as a soft segment was synthesized. The changes in the physical-mechanical and thermal properties of the materials observed in the course of a 36-month storage at room temperature were related to the corresponding structural evolution. The latter was monitored using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), wide-angle X-ray diffraction (WAXS) and mechanical tests (tensile strength test). The effects of the composition of the soft segment on the phase separation and crystallization of the soft segment were analyzed in detail. It was found that the melting temperature of the crystalline phase increases with storage time, which is associated with hindering of the phase separation of the hard and soft segments of the TPU samples as it was detected by FTIR.
Validation of the Prognostic Role for Surgical Treatment in Stage II Intrahepatic Cholangiocarcinoma: A SEER Population-Based Study

Background: This study aimed to determine the role of surgical treatment in patients with stage II intrahepatic cholangiocarcinoma (iCCA). Methods: Data were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. We divided stage II iCCAs into solitary tumors with vascular invasion (T2sN0M0) and multiple tumors with/without vascular invasion (T2mN0M0) according to the criteria of AJCC v.8. The Kaplan–Meier method and log-rank test were used to evaluate differences in overall survival (OS). We performed two propensity score-matching analyses with (PSM2) or without (PSM1) surgical treatment. Results: 667 and 778 iCCA patients with stage II and IIIB were recruited. After PSM2, there was no survival difference in stage II iCCA patients in hypothetical conditions with similar surgical proportions (p = 0.079). However, OS was significantly worse in patients with T2mN0M0 than T2sN0M0 when the actual surgical proportion existed after PSM1 (p < 0.001). OS was similar between T2mN0M0 and IIIB regardless of whether PSM1 (p = 0.907) or PSM2 (p = 0.699) was performed. The surgical treatment was verified to associate with prognosis. Conclusions: The survival benefit by surgical treatment was existed in Stage II but not in Stage IIIB iCCA patients. The OS for T2mN0M0 will approach that of T2sN0M0 if the surgical proportion is gradually increased.
Electron energy levels in ZnSe quantum dots

The size dependence of electron energy levels of ZnSe quantum dots were studied by size selective photoluminescence excitation spectroscopy at low temperature. ZnSe quantum dots of different sizes were synthesized by a high temperature wet chemical route. Cubic zinc-blende crystallites with sizes ranging from 1.5to4. 5nm showing only band edge luminescence were obtained. Four excited state transitions have been observed in photoluminescence excitation spectroscopy. This study establishes a connection between the electron energy levels of ZnSe quantum dots and their corresponding size. The experimentally observed excited states for ZnSe quantum dots have been analyzed on the basis of “effective mass approximation” calculations. The observed transitions for wide band gap ZnSe are compared with the well studied material, viz., CdSe. The present work enables one to gain further insight into the size dependence of the electronic structure of semiconductor quantum dots.
A FAINT NOVA IN THE NEBULA OF ANDROMEDA

A Suspected Nova in N. G. C. 2841 A photograph, with an exposure of two hours, made at the 25-foot focus of the 60-inch reflector on February 19, 1912, shows a relatively bright nebulous star in the spiral nebula N. G. C. 2841 that does not appear on earlier and later plates made at the Lick Observatory. The object is approximately 5o west and 20* north of the nucleus of the spiral; it is shown distinctly in the reproduction in the Astrophysical Journal, 46, Plate V a, 1917 (Mt. Wilson Conlr., No. 132). Professor Curtis independently found the star from an examination of the published photographs. He writes that on plates made with the Crossley reflector on April 17, 1901 (reproduced in Lick Publication, 8, Plate 19), and November 19, 1914, no trace of the star is seen. Observations will be continued when the spiral comes into position again. Francis G. Pease.
Mechanisms of Individual and Simultaneous Adsorption of Antibiotics and Dyes onto Halloysite Nanoclay and Regeneration of Saturated Adsorbent via Cold Plasma Bubbling

Halloysite nanoclay (HNC) was examined as an adsorbent for the individual and simultaneous removal of antibiotic enrofloxacin (ENRO) and methylene blue (MB) from aqueous solutions, alongside its regeneration via cold atmospheric plasma (CAP) bubbling. Initially, batch kinetics and isotherm studies were carried out, while the effect of several parameters was evaluated. Both ENRO and MB adsorption onto HNC was better described by Langmuir model, with its maximum adsorption capacity being 34.80 and 27.66 mg/g, respectively. A Pseudo-second order model fitted the experimental data satisfactorily, suggesting chemisorption (through electrostatic interactions) as the prevailing adsorption mechanism, whereas adsorption was also controlled by film diffusion. In the binary system, the presence of MB seemed to act antagonistically to the adsorption of ENRO. The saturated adsorbent was regenerated inside a CAP microbubble reactor and its adsorption capacity was re-tested by applying new adsorption cycles. CAP bubbling was able to efficiently regenerate saturated HNC with low energy requirements (16.67 Wh/g-adsorbent) in contrast to Fenton oxidation. Most importantly, the enhanced adsorption capacity of the CAP-regenerated HNC (compared to raw HNC), when applied in new adsorption cycles, indicated its activation during the regeneration process. The present study provides a green, sustainable and highly effective alternative for water remediation where pharmaceutical and dyes co-exist.