Miracle-dz/UniFAR-FTUs huggingface dataset:
Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells

Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource intensive instrumentation. Handheld devices, such as smartphones, are now routinely produced with CPUs, RAM, wireless data transfer capabilities, and high-resolution complementary metal oxide semiconductor (CMOS) cameras capable of supporting the capture and processing of bioluminescent signals. In theory, combining the capabilities of these devices with continuously bioluminescent human cell-based bioreporters would allow them to replicate the functionality of more expensive, more complex, and less flexible platforms while supporting human-relevant conclusions. In this work, we compare the performance of smartphone (CMOS) and night vision (image intensifier) devices with in vivo (CCD camera), and in vitro (photomultiplier tube) laboratory instrumentation for monitoring signal dynamics from continuously bioluminescent human cellular models under toxic, stable, and induced expression scenarios. All systems detected bioluminescence from cells at common plating densities. While the in vivo and in vitro systems were more sensitive and detected signal dynamics representing cellular health changes earlier, the night vision and smartphone systems also detected these changes with relatively similar coefficients of variation and linear detection capabilities. The smartphone system did not detect transcriptional induction. The night vision system did detect transcriptional activation, but was less sensitive than the in vivo or in vitro systems and required a stronger induction before the change could be resolved.
The Adaptation Model Offers a Challenge for the Predictive Coding Account of Mismatch Negativity

An unpredictable stimulus elicits a stronger event-related response than a high-probability stimulus. This differential in response magnitude is termed the mismatch negativity (MMN). Over the past decade, it has become increasingly popular to explain the MMN terms of predictive coding, a proposed general principle for the way the brain realizes Bayesian inference when it interprets sensory information. This perspective article is a reminder that the issue of MMN generation is far from settled, and that an alternative model in terms of adaptation continues to lurk in the wings. The adaptation model has been discounted because of the unrealistic and simplistic fashion in which it tends to be set up. Here, simulations of auditory cortex incorporating a modern version of the adaptation model are presented. These show that locally operating short-term synaptic depression accounts both for adaptation due to stimulus repetition and for MMN responses. This happens even in cases where adaptation has been ruled out as an explanation of the MMN (e.g., in the stimulus omission paradigm and the multi-standard control paradigm). Simulation models that would demonstrate the viability of predictive coding in a similarly multifaceted way are currently missing from the literature, and the reason for this is discussed in light of the current results.
Neutrophil Extracellular Traps in Digestive Cancers: Warrior or Accomplice

Characterized as a complex of extracellular DNA fibers and granule proteins, neutrophil extracellular traps (NETs) are generated specifically by neutrophils which play a critical role in host defense and immune regulation. NETs have been initially found crucial for neutrophil anti-microbial function. Recent studies suggest that NETs are involved in tumorigenesis and cancer progression. However, the function of NETs in cancer remains unclear, which might be due to the variation of research models and the heterogeneity of cancers. Although most of malignant tumors have similar biological behaviors, significant differences indeed exist in various systems. Malignant tumors of the digestive system cause the most incidence and mortality of cancer worldwide. In this review, we would focus on research developments on NETs in digestive cancers to provide insights on their role in digestive cancer progression and future research directions.
Roles for the 8-Oxoguanine DNA Repair System in Protecting Telomeres From Oxidative Stress

Telomeres are protective nucleoprotein structures that cap linear chromosome ends and safeguard genome stability. Progressive telomere shortening at each somatic cell division eventually leads to critically short and dysfunctional telomeres, which can contribute to either cellular senescence and aging, or tumorigenesis. Human reproductive cells, some stem cells, and most cancer cells, express the enzyme telomerase to restore telomeric DNA. Numerous studies have shown that oxidative stress caused by excess reactive oxygen species is associated with accelerated telomere shortening and dysfunction. Telomeric repeat sequences are remarkably susceptible to oxidative damage and are preferred sites for the production of the mutagenic base lesion 8-oxoguanine, which can alter telomere length homeostasis and integrity. Therefore, knowledge of the repair pathways involved in the processing of 8-oxoguanine at telomeres is important for advancing understanding of the pathogenesis of degenerative diseases and cancer associated with telomere instability. The highly conserved guanine oxidation (GO) system involves three specialized enzymes that initiate distinct pathways to specifically mitigate the adverse effects of 8-oxoguanine. Here we introduce the GO system and review the studies focused on investigating how telomeric 8-oxoguanine processing affects telomere integrity and overall genome stability. We also discuss newly developed technologies that target oxidative damage selectively to telomeres to investigate roles for the GO system in telomere stability.
Cilium Expression Score Predicts Glioma Survival

The accurate classification, prognostication, and treatment of gliomas has been hindered by an existing cellular, genomic, and transcriptomic heterogeneity within individual tumors and their microenvironments. Traditional clustering is limited in its ability to distinguish heterogeneity in gliomas because the clusters are required to be exclusive and exhaustive. In contrast, biclustering can identify groups of co-regulated genes with respect to a subset of samples and vice versa. In this study, we analyzed 1,798 normal and tumor brain samples using an unsupervised biclustering approach. We identified co-regulated gene expression profiles that were linked to proximally located brain regions and detected upregulated genes in subsets of gliomas, associated with their histologic grade and clinical outcome. In particular, we present a cilium-associated signature that when upregulated in tumors is predictive of poor survival. We also introduce a risk score based on expression of 12 cilium-associated genes which is reproducibly informative of survival independent of other prognostic biomarkers. These results highlight the role of cilia in development and progression of gliomas and suggest potential therapeutic vulnerabilities for these highly aggressive tumors.
Association of Cigarette Smoking With Male Cognitive Impairment and Metal Ions in Cerebrospinal Fluid

Objective: Cigarette smoking might accelerate cognitive impairment; however, this has never been investigated using human cerebrospinal fluid (CSF). We conducted this study to investigate the association between cigarette smoking and cognitive impairment through metal ions in CSF. Methods: We obtained 5-ml CSF samples from routine lumbar puncture procedures in patients undergoing anterior cruciate ligament reconstruction before surgery in China. A total of 180 Chinese males were recruited (80 active smokers and 100 non-smokers). We measured specific cigarette-related neurotoxic metal ions in CSF, including iron, copper, zinc, lead, aluminum, and manganese. Sociodemographic data and history of smoking were obtained. The Montreal Cognitive Assessment (MoCA) was applied. Results: Active smokers had fewer years of education (11.83 ± 3.13 vs. 13.17 ± 2.60, p = 0.01), and higher age (33.70 ± 10.20 vs. 29.76 ± 9.58, p = 0.01) and body mass index (25.84 ± 3.52 vs. 24.98 ± 4.06, p =0.03) than non-smokers. Compared to non-smokers, active smokers had significantly higher CSF levels of iron, zinc, lead, and aluminum and lower MoCA scores (all p < 0.05). Average daily numbers of cigarettes smoked negatively correlated with the MoCA scores (r = −0.244, p = 0.048). In young smokers, CSF manganese levels negatively correlated with MoCA scores (r = −0.373, p = 0.009). Conclusions and Relevance: Cigarette smoking might be associated with male cognitive impairment, as shown by lower MoCA scores and higher levels of CSF iron, zinc, lead, and aluminum in active smokers. This might be early evidence of cigarette smoking accelerating male cognitive impairment.
Molecular Pathology of Sodium Channel Beta-Subunit Variants

The voltage-gated Na+ channel regulates the initiation and propagation of the action potential in excitable cells. The major cardiac isoform NaV1.5, encoded by SCN5A, comprises a monomer with four homologous repeats (I-IV) that each contain a voltage sensing domain (VSD) and pore domain. In native myocytes, NaV1.5 forms a macromolecular complex with NaVβ subunits and other regulatory proteins within the myocyte membrane to maintain normal cardiac function. Disturbance of the NaV complex may manifest as deadly cardiac arrhythmias. Although SCN5A has long been identified as a gene associated with familial atrial fibrillation (AF) and Brugada Syndrome (BrS), other genetic contributors remain poorly understood. Emerging evidence suggests that mutations in the non-covalently interacting NaVβ1 and NaVβ3 are linked to both AF and BrS. Here, we investigated the molecular pathologies of 8 variants in NaVβ1 and NaVβ3. Our results reveal that NaVβ1 and NaVβ3 variants contribute to AF and BrS disease phenotypes by modulating both NaV1.5 expression and gating properties. Most AF-linked variants in the NaVβ1 subunit do not alter the gating kinetics of the sodium channel, but rather modify the channel expression. In contrast, AF-related NaVβ3 variants directly affect channel gating, altering voltage-dependent activation and the time course of recovery from inactivation via the modulation of VSD activation.
Lowpass filter with in-line beeline CMRC

A novel beeline compact microstrip resonant cell (BCMRC) is proposed. Its photonic bandgap and slow-wave effect are evaluated. A lowpass filter, consisting of three BCMRCs connected in series, exhibits advantages of low insertion loss in the passband, sharp, high and wide rejection in the stopband and compact size. Experimental and simulated results match well.
Theorizing Community for Sport Management Research and Practice

Community is a context for much research in sport, sport management, and sport policy, yet relatively few authors explicitly articulate the theoretical frameworks with which they interrogate the concept. In this paper, we draw from communitarian theory and politics in order to contribute to a robust discussion and conceptualization of community in and for sport management research and practice. We provide a synthesis of current sport management and related research in order to highlight contemporary theoretical and methodological approaches to studying community. We distinguish between community as a context, as an outcome, as a site for struggle or resistance, as well as a form of regulation or social control. We then advance a critical communitarian agenda and consider the practical implications and considerations for research and practice. This paper synthesizes current research and establishes a foundation upon which sport management scholars and practitioners might critically reflect on community and deliberatively articulate its implications in both future research and practice.
UNITY 7: GUI BAESD DESIGN PLATFORM FOR UBUNTU TOUCH MOBILE OS

Day by day the mobile industry is growing. Along with its mass usage the applications used in these devices are also increasing. So it is of utmost importance that the GUI should be fast, fresh and user friendly. Unity 7 is the GUI which provides the platform for the Touch interface for the Ubuntu OS. It falls under the category of open source; hence it doesn’t required runtime licenses. Unity is not a collection of applications but is designed to use existing programs. Traditional Linux uses command-driven interface (or text-based interface). By adopting the X-Window technology, graphical user interface (GUI) is available for Linux. It may be Menu-driven or Icon-driven. This paper shows the complete design of the Unity 7 GUI which includes the designing and implementation of Application framework, Application manager. This paper also shows the complete source code for the designing of the unity 7 GUI, its step by step building along with the different components used to build the Unity 7.
Angiogenesis, Anti-Tumor, and Anti-Metastatic Activity of Novel α-Substituted Hetero-Aromatic Chalcone Hybrids as Inhibitors of Microtubule Polymerization

A library of new heteroaromatic ring-linked chalcone analogs were designed and synthesized of these, compound 7m with α-CH3 substitution and bearing a benzofuran ring, displaying the most potent activity, with IC50 values of 0.07–0.183 µM against three cancer cells. Its low cytotoxicity toward normal human cells and strong potency on drug-resistant cells revealed the possibility for cancer therapy. It also could moderately inhibit in vitro tubulin polymerization with an IC50 value of 12.23 µM, and the disruption of cellular architecture in MCF-7 cells was observed by an immunofluorescence assay. Cellular-based mechanism studies elucidated that 7m arrested the cell cycle at the G2/M phase and induced apoptosis by regulating the expression levels of caspases and PARP protein. Importantly, the compound 7 m was found to inhibit HUVEC tube formation, migration, and invasion in vitro. In vivo assay showed that 7m could effectively destroy angiogenesis of zebrafish embryos. Furthermore, our data suggested that treatment with 7m significantly reduced MCF-7 cell metastasis and proliferation in vitro and in zebrafish xenograft. Collectively, this work showed that chalcone hybrid 7m deserves further investigation as dual potential tubulin polymerization and angiogenesis inhibitor.
the Undulating Distribution Channel

Recent management literature is littered with unfulfilled predictions and inaccurate assessments of how electronic commerce will alter distribution channels. The following article adds clarity to our understanding of the role of e-commerce in restructuring channels by examining a variety of scenarios where collapse, shift, and expansion of channels has occurred. Classical and new theory is used to interpret weak, failed, and successful models employed by a wide variety of established firms and e-commerce startups. COPYRIGHT NOTICE
The Structural Biology of Septins and Their Filaments: An Update

In order to fully understand any complex biochemical system from a mechanistic point of view, it is necessary to have access to the three-dimensional structures of the molecular components involved. Septins and their oligomers, filaments and higher-order complexes are no exception. Indeed, the spontaneous recruitment of different septin monomers to specific positions along a filament represents a fascinating example of subtle molecular recognition. Over the last few years, the amount of structural information available about these important cytoskeletal proteins has increased dramatically. This has allowed for a more detailed description of their individual domains and the different interfaces formed between them, which are the basis for stabilizing higher-order structures such as hexamers, octamers and fully formed filaments. The flexibility of these structures and the plasticity of the individual interfaces have also begun to be understood. Furthermore, recently, light has been shed on how filaments may bundle into higher-order structures by the formation of antiparallel coiled coils involving the C-terminal domains. Nevertheless, even with these advances, there is still some way to go before we fully understand how the structure and dynamics of septin assemblies are related to their physiological roles, including their interactions with biological membranes and other cytoskeletal components. In this review, we aim to bring together the various strands of structural evidence currently available into a more coherent picture. Although it would be an exaggeration to say that this is complete, recent progress seems to suggest that headway is being made in that direction.
A TWO-DIMENSIONAL MODELLING FOR HUMAN HIP STRESS ANALYSIS

This paper, based on the elasticity theory, a two dimensional modelling for the hip stress analysis in the sagittal plane is presented. Though the stress, in the normal human hip, is distributed over the entire crossection of the femur head, the main activities of the human being are limited in the sagittal plane. Moreover, a 2-D modelling for the stress analysis is much simpler and easier than 3-D one; especially it can be combined with the clinical standard anterior posterior rentgenographs. These predicate that it is useful and necessary to develop a 2-D modelling for the hip stress analysis. The validation of current modelling is verified by examining an example. In other words, the present modelling can be readily applied in clinical practice to estimate the peak stress in the most frequent body positions of everyday activities.
Design of high-performance all-fiber spectral filter with weighted coupling

Narrow-band and low-sidelobe all-fiber spectral filters are achievable with directional couplers constructed with W-index and step-index fibers. The sidelobe levels can be reduced significantly by properly weighting the coupling strength along the device. We provide numerical simulations in which the different weighting functions are obtained via a variable intercore separation. With a Gaussian function weighting, -38 dB transfer response sidelobes have been obtained, which is more than 29 dB reduction over the uniform coupling case. Some design rules for obtaining filters with desired bandwidths and coupling lengths are also given. These results can be very useful in the design of high-performance all-fiber filters for wavelength multiplexing. >
VIRTUAL REALITY FOR SUPPORTING THE CONFIGURATION OF TRANSFORMABLE ASSEMBLY SYSTEMS

Virtual Reality (VR) made its way out of research labs into industrial applications like product design, design for assembly, training issues, etc. Nevertheless, VR is still suffering from lacking technology to highlight its huge amount of functionality. That means the utilization of VR in a holistic way to combine the real and the virtual world to close the circle, in which the user can switch between both systems is still not common. There is more VR can contribute than an immersive 3D model of real objects and some interaction technology with different devices like 6 DOF mouse, PhantomTM, gloves with tactile feedback, etc. If you want to use VR like the usual technology, you have to integrate the features of 3D, interaction, force feedback, simulation and the real system. The research work to develop such an integrated system is done within a special research project (SFB 467) of the German Research Society (DFG) called "Transformable business structures for multiple-variant series production".
Compact Ultra-Wideband Conductor-Backed Coplanar Waveguide Bandpass Filter With a Dual Band-Notched Response

This work presents a novel ultra-wideband (UWB) bandpass filter (BPF) based on a conductor-backed coplanar waveguide structure with tunable transmission zeros. The symmetrical UWB BPF, which consists of the broadside-coupled transitions and the stub resonators in double-layer configuration, achieves a UWB bandwidth with transmission zeros. For characterizing this structure, the equivalent-circuit model is established to realize a four-pole response with two transmission zeros located close to the passband edges. To eliminate the interference of the coexisting wireless local area network (WLAN) within the UWB spectrum, two slotlines are symmetrically arranged on the ground plane of UWB BPF to generate the band-notched frequencies at 5.2 and 5.8 GHz simultaneously. The proposed UWB BPFs have the advantages of compact size, low insertion loss, good selectivity, and flat group delay. All results obtained from the equivalent-circuit model and the full-wave simulation are verified by measurements.
S2045796021000640jra 1..8

Aims. People with psychotic disorders receive mental healthcare services mainly for their psychiatric care needs. However, patients often experience multiple physical or social wellbeingrelated care needs as well. This study aims to identify care needs, investigate their changes over time and examine their association with mental healthcare consumption and evidence-based pharmacotherapy. Methods. This study combined annually obtained routine outcome monitoring (ROM) data with care consumption data of people with a long-term psychotic illness receiving treatment in four Dutch mental healthcare institutes between 2012 and 2016. Existing treatment algorithms were used to determine psychiatric, physical and social wellbeing-related care needs based on self-report questionnaires, semi-structured interviews and physical parameters. Care consumption was measured in hours of outpatient mental healthcare consumption per year. Generalised estimating equation models were used to calculate odds ratios of care needs and their associations with time, mental healthcare consumption and medication use. Results. Participants (n = 2054) had on average 7.4 care needs per measurement and received 25.4 h of care per year. Physical care needs are most prevalent and persistent and people with more care needs receive more mental healthcare. Care needs for psychotic symptoms and most social wellbeing-related care needs decreased, whereas the chance of being overweight significantly increased with subsequent years of care. Several positive associations were found between care needs and mental healthcare consumption as well as positive relations between care needs and evidence-based pharmacotherapy. Conclusions. This longitudinal study present a novel approach in identifying care needs and their association with mental healthcare consumption and pharmacotherapy. Identification of care needs in this way based on ROM can assist daily clinical practice. A recovery-oriented view and a well-coordinated collaboration between clinicians and general practitioners together with shared decisions about which care needs to treat, can improve treatment delivery. Special attention is required for improving physical health in psychosis care which, despite appropriate pharmacotherapy and increasing care consumption, remains troublesome.