Miracle-dz/UniFAR-FTUs huggingface dataset:
In Vitro Antiviral Activity and Cross-Resistance Proﬁle of PL-100, a Novel Protease Inhibitor of Human Immunodeﬁciency Virus Type 1 (cid:1)

Despite the success of highly active antiretroviral therapy, the current emergence and spread of drug- resistant variants of human immunodeﬁciency virus (HIV) stress the need for new inhibitors with distinct properties. We designed, produced, and screened a library of compounds based on an original L -lysine scaffold for their potentials as HIV type 1 (HIV-1) protease inhibitors (PI). One candidate compound, PL-100, emerged as a speciﬁc and noncytotoxic PI that exhibited potent inhibition of HIV-1 protease and viral replication in vitro ( K i , (cid:2) 36 pM, and 50% effective concentration [EC 50 ], (cid:2) 16 nM, respectively). To conﬁrm that PL-100 possessed a favorable resistance proﬁle, we performed a cross-resistance study using a panel of 63 viral strains from PI-experienced patients selected for the presence of primary PI mutations known to confer resistance to multiple PIs now in clinical use. The results showed that PL-100 retained excellent antiviral activity against almost HIV-1 PR. The inhibitor con- stant ( K i ) of each PI determined by incubating bacterially expressed, puriﬁed HIV-1 PR (Bachem) at 31°C in reaction buffer (1 M NaCl, 100 mM Na acetate, 1 mM EDTA, 1 mM dithiothreitol, and 1 mg/ml bovine serum albumin, pH 5.2) containing increasing concentrations of test inhibitor. After a 5-min preincuba- tion, the reaction was initiated by the addition of the ﬂuorogenic substrate Arg-Glu (EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys (DABCYL)-Arg (Molecular Probes) at a ﬁnal concentration of 10 (cid:3) M. Cleavage of the substrate was monitored by measuring the increase in the ﬂuorescence intensity at 490 nm after excitation at 340 nm (16) using an FL600 ﬂuorometer (BioTek). K i values were obtained by ﬁtting the initial velocity data to the tightly binding inhibition equation as described 50% inhibitory concentrations (IC 50 s) were determined from a dose-response curve and converted to K i s using the equation IC 50 (cid:5) K i (1 (cid:1) [ S ]/ K m ), where [ S ] is the substrate concentration and K m , the Michaelis-Menten constant. the K i PL-100 on puriﬁed cathepsin D and pepsin. cathepsin D (Calbiochem) PL-100 ﬂuorescent (Bachem). Brieﬂy initiated ﬁnal (cid:3) enzyme
Cognitive and Psycho-Social Effects of Childhood Obesity

Childhood obesity has been declared as a global epidemic. Several studies have reported inverse associations of childhood obesity with cognitive and psycho-social functioning of obese children. The present study is an attempt to understand the cognitive and psycho-social aspects of childhood obesity in Indian context. The objective of the study was to compare the cognitive functions (memory, attention, and visual retention) and psychosocial functioning (body shape concerns and insecurity) of obese children with normal weight controls. The cross sectional research was carried out. Purposive sampling was used to collect the sample of 30 obese children and 30 normal weight children (controls) from schools in Delhi. The inclusion criteria for cases and controls were obese and normal weight children as per ICMR criteria, within the age range of 10-14 years. Children with previous history of head injury or other physical, medical or neurological problems, chronic medical illness and other metabolic disorders (diabetes and thyroid), were excluded from the study. All the children were assessed on cognitive functions (Digit symbol test and immediate memory test) and psycho-social functioning (Body Shape Questionnaire, Mohsen’s security insecurity questionnaire). The performance of obese children was found to be poor compared to normal weight children by using t-test. Obese children performed significantly poor on attention and visual retention and immediate memory for direct reciprocation. However, there was no significant difference on reverse immediate memory test. Obese children were also found to be more insecure compared to the normal weight controls about their body image. Obese children expressed high body shape concerns compared to normal weight counterparts. Findings suggest independent associations of specific cognitive and psychosocial aspects with childhood obesity that can be targeted for prevention and management of this global epidemic.
Cycloheximide. A Protein Synthesis Inhibitor with Teratogenic, Embryotoxic and Carcinogenetic Expression cycloheximide: a protein synthesis inhibitor with teratogenic, embryotoxic and carcinogenetic expression

: Cycloheximide [4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl] -2-hydroxyethyl}piperidine-2,6-dione] and Molecular formula C 15 H 23 NO 4 , is a highly toxic agent and a protein synthesis inhibitor that acts specifically on the 60S subunit of eukaryotic ribosomes. Cycloheximide is a widely used fungicide, which must be very carefully applied due to diverse side effects possessing. Mitomycin C (MMC) and cycloheximide (CHX) are known for their apoptotic and antitumor activity. CHX is also known for its property to inhibit protein synthesis and to reduce cytotoxicity of various antitumor drugs, i.e. inducing an adaptive survival response (ASR). Cycloheximide (CHX) can contribute to apoptotic processes, either in conjunction with another agent (e.g. tumor necrosis factor-alpha) or on its own. Cyclohexamide is involved in the inhibition of truly mitochondrial protein synthesis. Cycloheximide, as a protein synthesis inhibitor, was studied in breast cancer cell lines. Cycloheximide is teratogenic in litters treated with this and the teratogenic manifestations that produces are polydactyly, oligodactyly, and a variety of skeletal abnormalities including incidence of taillessness, as well as gross malformations and total skeletal malformations were identified. Prenatal mortality and decreased foetal weights was also observed in treated litters. Cycloheximide exerts a potent estrogenic expression, interpreted at breast and uterine epithelial cell cancer lines studied. από τα μιτοχόνδρια. Επίσης, ως αναστολέας της σύνθεσης των πρωτεϊνών έχει μελετηθεί καρ-κινικών κυττάρων του μαστού. Θεωρείται ως ισχυρός τερατογόνος παράγοντας, με έκφραση της κυρίως στον σκελετό των θηλαστικών. Έτσι μετά από εφαρμογή της σε πειραματόζωα συγγενών ανωμαλιών του σκελετού του κορμού Απόπτω-ση, οιστρογόνων.
Epidemiological Study of Symptomatic and Asymptomatic Bacteriuria Among Pregnant Women Attending Antenatal Clinic in Baquba- Diyala Province

Background: Pregnancy causes many changes in the pregnant women, due to mechanical and hormonal changes which lead to ureteral dilatation and urinary stasis which contribute to the increased risk of developing urinary tract infection. Subject and methods: A cross sectional study was conducted at Al-Batool Teaching Hospital in antenatal care clinic from March during October 2012 to study the epidemiology of symptomatic and asymptomatic bacteriuria among pregnant women. Results: out of 200 pregnant women who were included (24%) and (14%) with ≤20 years old age group were asymptomatic and symptomatic respectively, and (56%), (64%) were asymptomatic and symptomatic in age group 21-30 years old , while in those ≥ 31 years age group was (20%) and (22%) asymptomatic and symptomatic respectively. The asymptomatic bacteriuria was (77%) and it was higher among 31-40 weeks of gestation than other weeks of gestations (p= 0.006). The nulliparous was (27%) asymptomatic and (21%) symptomatic, where para1- para3 was (56%) asymptomatic and (65%) symptomatic and para4 and above was (17%) asymptomatic and (14%) symptomatic. Escherichia coli was (42.3%) more than other species. Conclusion: Urinary tract infections is a public health problem, Routine urine culture test should be performed on all antenatal asymptomatic and symptomatic pregnant women to identify unsuspected infection.
Cytoplasmic Domain of Inﬂuenza B Virus BM2 Protein Plays Critical Roles in Production of Infectious Virus (cid:1)

Inﬂuenza B virus BM2 is a type III integral membrane protein that displays H (cid:1) ion channel activity. Analysis of BM2 knockout mutants has suggested that this protein is a necessary component for the capture of M1-viral ribonucleoprotein (vRNP) complex at the plasma membrane and for incorporation of vRNP complex into the virion during the assembly process. BM2 comprises 109 amino acid residues and possesses a longer cytoplasmic domain than the other 3 integral membrane proteins (hemagglutinin, neuraminidase, and NB). To explore whether the cytoplasmic domain of BM2 is important for infectious virus production, a series of BM2 deletion mutants lacking three to nine amino acid residues at the carboxyl terminus, BM2 (cid:2) 107-109, BM2 (cid:2) 104-109, and BM2 (cid:2) 101-109, was generated by reverse genetics. Intracellular transport and incorporation into virions were indistinguishable between truncated BM2 proteins and wild-type BM2. The BM2 (cid:2) 107-109 mutant produced levels of infectious virus similar to those of wild-type virus and displayed a spherical shape. However, the BM2 (cid:2) 104-109 and BM2 (cid:2) 101-109 mutants produced viruses containing dramatically reduced vRNP complex, as with BM2 knockout mutants, and formed enlarged, irregularly shaped virions. Moreover, gradient separation of membranes indicated that membrane association of M1 from mutants was greatly affected by carboxyl-terminal truncations of BM2. Studies of alanine substitution mutants further suggested that amino acid sequences in the 98–109 region are variable while those in the 86–97 region are a prerequisite for innate BM2 function. These results indicate that the cytoplasmic domain of the BM2 protein is required for ﬁrm association of the M1 protein with lipid membranes, vRNP complex incorporation into virions, and virion morphology.
Radar-Based Timed Up And Go Test For Multiperson Providing A Risk Of Falling Assessment

Objective: Over the past decade, the research community has made major advances in technologies for detecting risk of falling in elderly, which led to assistive systems that can help to improve physical strength and limit isolation. However, it is still expensive to extend these systems to a multi-user performing a clinical test, such as the Timed Up and Go (TUG). In this paper, we therefore propose a new avenue, a radar-based system that transcends these limitations. Results: The proposed methodology is evaluated using data from real-world scenarios. It is found that distinguishing two participants performing a TUG test at the same time is more accurate when they are walking in opposite direction compared to perfectly synchronized walking in the same direction. However, although it was more challenging to distinguish two participants in this latter scenario, our results show that when they are offset by a certain distance, the proposed algorithm can track them separately. Our goal was therefore to relieve congestion in medical settings by investigating the feasibility of administering a clinical test with more than one participant at the same time thanks to an UWB radar sensor.
Méthodes d'optimisation non differentiable pour la résolution de grands problèmes. Applications à la gestion à moyen-terme de la production.

Cette these s'interesse a la resolution de problemes d'optimisation non-differentiable de grandes tailles resultant le plus souvent d'une relaxation Lagrangienne d'un probleme difficile. Cette technique est couramment utilisee pour apprehender des problemes lineaires avec nombres entiers ou des problemes convexes complexes. Le probleme dual obtenu est non-differentiable - eventuellement separable - et peut etre resolu par exemple par un algorithme de faisceau. Le Chapitre 2 propose une revue de litterature des methodes d'optimisation non-differentiable. Dans certaines situations, le probleme dual peut etre lui-meme tres difficile a resoudre et necessiter des strategies adaptees. Par exemple, lorsque le nombre de contraintes dualisees est tres eleve, une dualisation explicite peut s'averer impossible ou la mise a jour des variables duales peut echouer. Au Chapitre 3, nous etudions les proprietes de convergence lorsqu'une relaxation Lagrangienne dynamique est effectuee : seul un sous-ensemble de contraintes est dualise a chaque iteration, ce qui permet de reduire la dimension du probleme dual. Une autre limite de la relaxation Lagrangienne peut apparaitre lorsque la fonction duale est separable en un grand nombre de sous fonctions, ou que celles-ci restent difficiles a evaluer. Une strategie naturelle consiste alors a tirer partie de la structure separable en effectuant des iterations duales en n'ayant evalue qu'un sous-ensemble des sous fonctions. Au chapitre 4, nous proposons d'utiliser une methode de faisceau dans ce contexte incremental. Enfin, le Chapitre 5 presente des applications numeriques sur des problemes de gestion de production d'electricite.
Goal priming as a situated intervention tool Esther K Papies 1,2

Research on goal priming has shown that cues in the environment can lead to goal-directed cognition and behaviour without the need for conscious intentions. This has sparked an interest in using goal priming as an intervention tool to strategically inﬂuence behaviour in line with an individual’s long-term goals. The present article ﬁrst gives a brief overview of goal priming effects and their mechanisms. Then, goal priming is discussed as a situated intervention tool that changes the cognitive responses triggered by a situation and can stimulate the pursuit of long-term investment goals over short-term hedonic goals. Applying the principle of situating interventions leads to a set of recommendations for applying goal primes effectively, which are illustrated with examples from various domains. This thoughtful and thorough meta-analysis synthesizes 133 studies in which the effects of word primes on behaviour were examined. In addition to a small and robust priming effect across domains, the authors show that this effect is stronger when the primed concept is more valued, that goal priming effects seem to persist over somewhat extended periods of time, and that larger samples are needed for sufﬁciently powered future goal priming studies.
Pregnancy-speciﬁc b -1-glycoprotein 1 and human leukocyte antigen-E mRNA in human sperm: differential expression in fertile and infertile men and evidence of a possible functional role during early development

protein extracts ( n ¼ 3). Using ICSI of human sperm into hamster oocytes we evaluated the permanence of these mRNAs at different time points ( n ¼ 10 for each time) after fertilization. results: PSG1, HLA-E and PRM2 transcripts were demonstrated in ejaculated sperm. The fertile group showed signiﬁcantly higher levels of PSG1 and HLA-E mRNA (both P , 0.05) than the infertile group, whereas PRM2 levels were not signiﬁcantly different. However, PSG1 and HLA-E proteins were not found in ejaculated sperm. Following ICSI, PRM2 was undetectable after fertilization; conversely, PSG1 and HLA-E transcripts remained detectable for at least 24 h of zygotic development. conclusions: We provide new evidence that indicates that human sperm deliver transcripts that may have a role in early embryo development and decreased levels of these transcripts may be associated with infertility.
Institutional Knowledge at Singapore Management University Institutional Knowledge at Singapore Management University We can hear you with WiFi! We can hear you with WiFi!

Recent literature advances Wi-Fi signals to “see” people’s motions and locations. This paper asks the following ques-tion: Can Wi-Fi “hear” our talks? We present WiHear, which enables Wi-Fi signals to “hear” our talks without deploying any devices. To achieve this, WiHear needs to detect and analyze ﬁne-grained radio reﬂections from mouth movements. WiHear solves this micro-movement detection problem by introducing Mouth Motion Proﬁle that leverages partial multipath eﬀects and wavelet packet transformation. Since Wi-Fi signals do not require line-of-sight, WiHear can “hear”people talks within the radio range. Further, WiHear can simultaneously “hear” multiple people’s talks leveraging MIMO technology. We implement WiHear on both USRP N210 platform and commercial Wi-Fi infrastructure. Results show that within our pre-deﬁned vocabulary, WiHear can achieve detection accuracy of 91% on average for single individual speaking no more than 6 words and up to 74% for no more than 3 people talking simultaneously. Moreover, the detection accuracy can be further improved by deploying multiple receivers from diﬀerent angles.
Kinetic proﬁle of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-speciﬁc inhibitors

Kinetic proﬁle of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-speciﬁc inhibitors. Am. J. Physiol. 276 ( Regulatory Integrative Comp. Physiol. 45): R1691–R1700, 1999. —20-Hydroxyeicosatetraenoic acid (HETE), the cytochrome P -450 (CYP) 4A v -hydroxylation product of arachidonic acid, has potent biological effects on renal tubular and vascular functions and on the control of arterial pressure. We have expressed high levels of the rat CYP4A1, -4A2, -4A3, and -4A8 cDNAs, using baculovirus and Sf9 insect cells. Arachidonic acid v - and v -1-hydroxylations were catalyzed by three of the CYP4A isoforms; the highest catalytic efficiency of 947 nM 2 1 ·min 2 1 for CYP4A1 was followed by 72 and 22 nM 2 1 ·min 2 1 for CYP4A2 and CYP4A3, respectively. CYP4A2 and CYP4A3 exhibited an additional arachidonate 11,12-epoxidation activity, whereas CYP4A1 operated solely as an v -hydroxylase. CYP4A8 did not catalyze arachidonic or linoleic acid but did have a detectable lauric acid v -hydroxylation activity. The inhibitory activity of various acetylenic and oleﬁnic fatty acid analogs revealed differences and indicated isoform-speciﬁc inhibition. These studies suggest that CYP4A1, despite its low expression in extrahe-patic tissues, may constitute the major source of 20-HETE synthesis. Moreover, the ability of CYP4A2 and -4A3 to catalyze the formation of two opposing biologically active metabolites, 20-HETE and 11,12-epoxyeicosatrienoic acid, may be of great signiﬁcance to the regulation of vascular tone.
Model Development Strategy for Improved Quality of Private Higher Education that Manage Diploma Program through the Implementation of Good University Governance

Indonesia's competitiveness ranking based on the Global Competitiveness Index (GCI) has not been so encouraging, especially of higher education. This study aims to analyze how the governance and quality of private universities tha manage diploma program; and to analyze the influence of good university governance on the quality of private universities, partially and simultaneously. This study is a de-scriptive-verification with design research is causality and cross-sectional. The population in this study is a Private Higher Education that manages diploma program. Where the unit sample is a lecturer. Data analysis techniques used in this research is path analysis. The results of this study indicate that the governance descriptive colleges included in the classification of a high score. In addition, the quality of higher education are in a high classification. In the verification results show that the good university governance has positive and significant impact on the quality of private higher education, either partially or simultaneously. These findings illustrate that the private higher education needs to improve its governance through the aspects of transparency, accountability, responsibility, independence and fairness.
Reply to J Monárrez-Espino and T Greiner

We read with interest the validation study for ferritin in capillary blood by Ahluwalia et al (1). As pointed out by the authors, there is a need for “tools that are applicable for field use in remote areas. ” Subsequent to the publication of the spot ferritin assay for samples dried on filter paper (2), we used this method to measure serum ferritin in a survey conducted in a very isolated indigenous group in northern Mexico (3) after adapting the method for use under field conditions (4). We would like to share some of the difficulties encountered when using this technique and reflect on its potential for use in remote settings. First, although the filter-paper method avoids the need to freeze samples, samples still need to be centrifuged. In our survey, we ran a microcentrifuge from a car battery with the use of a converter to transform 12-V direct current to 110-V alternating current. Transporting and using this equipment (weighing up to 25 kg) can be difficult in isolated locations. Second, breaking the microcapillary tube to extract the serum exactly between the serum and the white cell layer can cause spillage, especially if done by a nonexperienced person. Third, extracting 20 L serum accurately can be done only with an expensive ($200) micropipette fitted with a precision micrometer-adjustment device; otherwise, some measurement error is expected. Fourth, because of the small diameter of microcapillary tubes, the samples must be centrifuged immediately because the blood clots within minutes, potentially obstructing the extraction of serum. Using tubes with anticoagulant solves this problem, but measuring ferritin in plasma rather than in serum will result in higher withinand between-sample variation (5). A deep prick with a long-tip lancet is needed to obtain an adequate amount of free-flowing capillary blood. After the prick, the first drop is wiped out, the second drop is used for measuring hemoglobin with a HemoCue (HemoCue, Inc, Mission Viejo, CA), and the last 3–4 drops are used to fill a 75L microcapillary tube. The amount of serum available diminishes as the hemoglobin concentration increases; thus, in persons with adequate iron status, a larger volume might be needed to provide the 20 L serum required for the analysis. A finger prick is often considered less invasive and more acceptable than is venipuncture. Although this could be true when obtaining 2 drops of blood, it might not be so if 6 or more drops are taken. Many of the women we surveyed stated that finger-prick sampling was more painful than was having venous blood drawn. In conclusion, specialized and relatively expensive equipment and well-trained personnel are needed to use the ferritin assay for capillary samples spotted on filter paper. These factors need to be taken into account when planning surveys in remote and isolated field settings. Joel Monárrez-Espino
Defining B-c, B* and B-phi for YBCO Thin Films

— The accommodation ﬁeld, , is generally deﬁned to be theﬁeldat which thecross overfrom singlevortexpinning tocol-lective pinning occurs. It is determined from magnetization curves as the point where the (cid:0) plateau ends and it used as a convenient way of comparing the pinning properties of superconducting ﬁlms. Similarly, the characteristic ﬁeld, , can be obtained from mag- neto-optical (MO) images from when the ﬂux fronts meet in the middle of the ﬁlm. The matching ﬁeld, , at which there is one vortex line per pinning site, is sometimes thought to be the same as , but in (cid:0)(cid:2)(cid:3)(cid:4)(cid:5) (cid:2) -doped (cid:6)(cid:0)(cid:2) (cid:3) (cid:7)(cid:8) (cid:2) (cid:5) (cid:4) ﬁlms the calculated is much higher than the observed . can be determined from angular dependent transport measurements. All of the ﬁeld values correspond to some special case in the ﬂux pinning in the ﬁlm and relate to (cid:0) . In this work we have determined , and for different kinds of YBCO ﬁlms using MO, magnetization and transport measurements to reveal the deeper meaning of the special ﬁelds.