Miracle-dz/UniFAR-FTUs huggingface dataset:
Will I die of coronavirus? Google Trends data reveal that politics determine virus fears

Is Google Trends (GT) useful to survey populations? Extant work has shown that certain search queries reflect the attitudes of hard-to-survey populations, but we do not know if this extends to the general population. In this article, we leverage abundant data from the Covid-19 pandemic to assess whether people’s worries about the pandemic match epidemiological trends as well as political preferences. We use the string ‘will I die from coronavirus’ on GT as the measure for people’s level of distress regarding Covid-19. We also test whether concern for coronavirus is a partisan issue by contrasting GT data and 2016 election results. We find strong evidence that (1) GT search volume close matches epidemiological data and (2) significant differences exist between states that supported Clinton or Trump in 2016.
Iron Deficiency and Anemia 10 Years After Roux-en-Y Gastric Bypass for Severe Obesity

Iron deficiency with or without anemia is a well-known long-term complication after Roux-en-Y, gastric bypass (RYGB) as the procedure alters the gastrointestinal absorption of iron. Iron is essential for hemoglobin synthesis and a number of cellular processes in muscles, neurons, and other organs. Ferritin is the best marker of iron status, and in a patient without inflammation, iron deficiency occurs when ferritin levels are below 15 µg/L, while iron insufficiency occurs when ferritin levels are below 50 µg/L. Lifelong regular blood tests are recommended after RYGB, but the clinical relevance of iron deficiency and iron insufficiency might be misjudged as long as the hemoglobin levels are normal. The aim of this study was to explore the frequency of iron deficiency and iron deficiency anemia one decade or more after RYGB, the use of per oral iron supplements, and the frequency of intravenous iron treatment. Nine hundred and thirty patients who underwent RYGB for severe obesity at three public hospitals in Norway in the period 2003–2009 were invited to a follow-up visit 10–15 years later. Results from blood tests and survey data on the use of oral iron supplements and intravenous iron treatment were analyzed. Ferritin and hemoglobin levels more than 10 years after RYGB were available on 530 patients [423 (79.8%) women]. Median (IQR) ferritin was 33 (16–63) µg/L, and mean (SD) hemoglobin was 13.4 (1.3) g/dl. Iron deficiency (ferritin ≤ 15 µg/L) was seen in 125 (23.6%) patients; in addition, iron insufficiency (ferritin 16–50 µg/L) occurred in 233 (44%) patients. Mean (SD) hemoglobin levels were 12.5 (1.4) g/dl in patients with iron deficiency, 13.5 (1.2) g/dl in patients with iron insufficiency, 13.8 (1.3) g/dl in the 111 (21%) patients with ferritin 51–100 µg/L, and 13.8 (1.2) g/dl in the 55 (10%) patients with ferritin >100 µg/L. Two hundred and seventy-five (56%) patients reported taking oral iron supplements, and 138 (27.5%) had received intravenous iron treatment after the RYGB procedure. Iron deficiency or iron insufficiency occurred in two-thirds of the patients 10 years after RYGB, although more than half of them reported taking oral iron supplements.
Task Complexity and Image Clarity Facilitate Motor and Visuo-Motor Activities in Mirror Therapy in Post-stroke Patients

Introduction: Mirror therapy is effective in the recovery of upper-limb function among post-stroke patients. An important component of mirror therapy is imagining finger movements. This study aimed to determine the influence of finger movement complexity and mirror image clarity on facilitating motor and visuo-motor activities in post-stroke patients. Methods: Fifteen post-stroke patients and 18 right-handed healthy participants performed simple or complex finger tapping while viewing mirror images of these movements at varying levels of clarity. The physical setup was identical to typical mirror therapy. Functional near infrared spectroscopy (fNIRS) was used to capture the brain activities elicited in the bilateral primary motor cortices (M1) and the precuneus using a block experimental design. Results: In both study groups, the “complex finger-tapping task with blurred mirror image” condition resulted in lower intensity (p < 0.01) and authenticity (p < 0.01) of the kinesthetic mirror illusion, and higher levels of perceived effort in generating the illusion (p < 0.01), relative to the “simple finger-tapping with clear mirror image” condition. Greater changes in the oxygenated hemoglobin (HbO) concentration were recorded at the ipsilesional and ipsilateral M1 in the “complex finger-tapping task with blurred mirror image” condition relative to that recorded in the “simple finger-tapping task with clear mirror image” condition (p = 0.03). These HbO concentration changes were not significant in the precuneus. Post-stroke patients showed greater changes than their healthy counterparts at the ipsilesional M1 (F = 5.08; p = 0.03; partial eta squared = 0.14) and the precuneus (F = 7.71; p < 0.01; partial eta squared = 0.20). Conclusion: The complexity and image clarity of the finger movements increased the neural activities in the ipsilesional motor cortex in the post-stroke patients. These findings suggest plausible roles for top-down attention and working memory in the treatment effects of mirror therapy. Future research can aim to corroborate these findings by using a longitudinal design to examine the use of mirror therapy to promote upper limb motor recovery in post-stroke patients.
Use of Immersive 3-D Virtual Reality Environments in Control Room Validations

Abstract Advanced technologies (e.g., virtual and augmented reality) may provide new possibilities to facilitate control room (CR) design and evaluation activities. We studied how immersive three-dimensional (3-D) virtual reality environments may augment and advance the evaluation of safety-critical nuclear power plant CR systems. A multiuser functionality enables several operators to be located and to collaborate in the same virtual CR environment at the same time. There is also a realistic representation of emergency operating procedures in the virtual CR. Spatial audio communication through headsets makes the experience even more realistic. The paper addresses both technical and human factors issues associated with the use of immersive 3-D virtual reality environments in CR validation tests, for example, the amount of technical resources required as compared to normal validation in a real-life physical simulator environment, creation of methodologically new testing opportunities, and new opportunities for data registration and analysis. A new framework has been established for estimating the needed fidelity level of the virtual CR for the type of system evaluation at hand.
Hybrid Pointer Networks for Combinatorial Optimization Problems

In this work, a novel idea is presented for combinatorial optimization problems, a hybrid network, which results in a superior outcome. We applied this method to graph pointer networks [1] expanding its capabilities to a higher level. We proposed a hybrid pointer network (HPN) to solve the travelling salesman problem trained by reinforcement learning. Furthermore, HPN builds upon graph pointer networks which is an extension of pointer networks with an additional graph embedding layer. HPN outperforms the graph pointer network in solution quality due to the hybrid encoder, which provides our model with a verity encoding type, allowing our model to converge to a better policy. Our network significantly outperforms the original graph pointer network for small and large-scale problems increasing its performance for TSP50 from 5.959 to 5.706 without utilizing 2opt, Pointer networks, Attention model, and a wide range of models, producing results comparable to highly tuned and specialized algorithms. We make our data, models, and code publicly available https://github.com/AhmedStohy/Hybrid-Pointer-Networks.
Visual perspective-taking in complex natural scenes

Studies of visual perspective-taking have shown that adults can rapidly and accurately compute their own and other peoples’ viewpoints, but they experience difficulties when the two perspectives are inconsistent. We tested whether these egocentric (i.e., interference from one’s own perspective) and altercentric biases (i.e., interference from another person’s perspective) persist in ecologically valid complex environments. Participants (N = 150) completed a dot-probe visual perspective-taking task, in which they verified the number of discs in natural scenes containing real people, first only according to their own perspective and then judging both their own and another person’s perspective. Results showed that the other person’s perspective did not disrupt self perspective-taking judgements when the other perspective was not explicitly prompted. In contrast, egocentric and altercentric biases were found when participants were prompted to switch between self and other perspectives. These findings suggest that altercentric visual perspective-taking can be activated spontaneously in complex real-world contexts, but is subject to both top-down and bottom-up influences, including explicit prompts or salient visual stimuli.
Transcriptional profiles of genes related to electrophysiological function in Scn5a +/− murine hearts

The Scn5a gene encodes the major pore‐forming Nav1.5 (α) subunit, of the voltage‐gated Na+ channel in cardiomyocytes. The key role of Nav1.5 in action potential initiation and propagation in both atria and ventricles predisposes organisms lacking Scn5a or carrying Scn5a mutations to cardiac arrhythmogenesis. Loss‐of‐function Nav1.5 genetic abnormalities account for many cases of the human arrhythmic disorder Brugada syndrome (BrS) and related conduction disorders. A murine model with a heterozygous Scn5a deletion recapitulates many electrophysiological phenotypes of BrS. This study examines the relationships between its Scn5a+/− genotype, resulting transcriptional changes, and the consequent phenotypic presentations of BrS. Of 62 selected protein‐coding genes related to cardiomyocyte electrophysiological or homeostatic function, concentrations of mRNA transcribed from 15 differed significantly from wild type (WT). Despite halving apparent ventricular Scn5a transcription heterozygous deletion did not significantly downregulate its atrial expression, raising possibilities of atria‐specific feedback mechanisms. Most of the remaining 14 genes whose expression differed significantly between WT and Scn5a+/− animals involved Ca2+ homeostasis specifically in atrial tissue, with no overlap with any ventricular changes. All statistically significant changes in expression were upregulations in the atria and downregulations in the ventricles. This investigation demonstrates the value of future experiments exploring for and clarifying links between transcriptional control of Scn5a and of genes whose protein products coordinate Ca2+ regulation and examining their possible roles in BrS.
Effects of Lignin Content on Mechanical and Thermal Properties of Polypropylene Composites Reinforced with Micro Particles of Spray Dried Cellulose Nanofibrils

In this work, the use of microsized cellulosic particles obtained from spray dried cellulose nanofibrils with high lignin content (>20 wt %) were explored for the first time as reinforcement in polypropylene (PP) composites. Their effect was compared with the results from PP composites reinforced by cellulosic particles of spray dried cellulose nanofibrils with a low lignin content (<5 wt %). Cellulose nanofibrils with diameters less than 100 nm were obtained by mechanically fibrillating unbleached and bleached cellulosic fibers obtained from tree bark after alkaline extraction for removal of extractive. These cellulose nanofibrils were then spray dried to microsized high lignin content cellulose particles (HLCP) and low lignin content cellulose particles (LLCP), respectively. The presence of a large amount of lignin in the nanofibrils alleviated the degree of aggregation during the spray drying process. Both HLCP and LLCP were melt compounded with polypropylene (PP) to make composites films with differen.
Freshwater wetland plants respond nonlinearly to inundation over a sustained period.

PREMISE Wetland plants regularly experience physiological stresses resulting from inundation; however, plant responses to the interacting effects of water level and inundation duration are not fully understood. METHODS We conducted a mesocosm experiment on two wetland species, sawgrass (Cladium jamaicense) and muhly grass (Muhlenbergia filipes), that co-dominate many freshwater wetlands in the Florida Everglades. We tracked photosynthesis, respiration, and growth at water levels of -10 (control), 10 (shallow), and 35 cm (deep) with reference to soil surface over 6 months. RESULTS The response of photosynthesis to inundation was nonlinear. Specifically, photosynthetic capacity (Amax ) declined by 25% in sawgrass and by 70% in muhly grass after 1-2 months of inundation. After 4 months, Amax of muhly grass in the deep-water treatment declined to near zero. Inundated sawgrass maintained similar leaf respiration and growth rates as the control, whereas inundated muhly grass suppressed both respiration and growth. At the end of the experiment, sawgrass had similar nonstructural carbohydrate pools in all treatments. By contrast, muhly grass in the deep-water treatment had largely depleted sugar reserves but maintained a similar starch pool as the control, which is critical for post-stress recovery. CONCLUSIONS Overall, the two species exhibited nonlinear and contrasting patterns of carbon uptake and use under inundation stress, which ultimately defines their strategies of surviving regularly flooded habitats. The results suggest that a future scenario with more intensive inundation, due to the water management and climate change, may weaken the dominance of muhly grass in many freshwater wetlands of the Everglades.
Analysis of the Expression Pattern of Cajal-Retzius Cell Markers in the Xenopus laevis Forebrain

Cajal-Retzius cells are essential for cortical development in mammals, and their involvement in the evolution of this structure has been widely postulated, but very little is known about their progenitor domains in non-mammalian vertebrates. Using in situhybridization and immunofluorescence techniques we analyzed the expression of some of the main Cajal-Retzius cell markers such as Dbx1, Ebf3, ER81, Lhx1, Lhx5, p73, Reelin, Wnt3a, Zic1, and Zic2 in the forebrain of the anuran Xenopus laevis, because amphibians are the only class of anamniote tetrapods and show a tetrapartite evaginated pallium, but no layered or nuclear organization. Our results suggested that the Cajal-Retzius cell progenitor domains were comparable to those previously described in amniotes. Thus, at dorsomedial telencephalic portions a region comparable to the cortical hem was defined in Xenopus based on the expression of Wnt3a, p73, Reelin, Zic1, and Zic2. In the septum, two different domains were observed: a periventricular dorsal septum, at the limit between the pallium and the subpallium, expressing Reelin, Zic1, and Zic2, and a related septal domain, expressing Ebf3, Zic1, and Zic2. In the lateral telencephalon, the ventral pallium next to the pallio-subpallial boundary, the lack of Dbx1 and the unique expression of Reelin during development defined this territory as the most divergent with respect to mammals. Finally, we also analyzed the expression of these markers at the prethalamic eminence region, suggested as Cajal-Retzius progenitor domain in amniotes, observing there Zic1, Zic2, ER81, and Lhx1 expression. Our data show that in anurans there are different subtypes and progenitor domains of Cajal-Retzius cells, which probably contribute to the cortical regional specification and territory-specific properties. This supports the notion that the basic organization of pallial derivatives in vertebrates follows a comparable fundamental arrangement, even in those that do not have a sophisticated stratified cortical structure like the mammalian cerebral cortex.
Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells

Abstract Objectives Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons. Methodology After isolation and characterization of mesenchymal stem cell identity, DPSCs were cultured in a NSPC induction medium and culture vessels. The durations of the culture, dissociation methods, and passage numbers of DPSCs were varied. Results Neurosphere formation requires a special surface that inhibits cell attachment. Five-days was the most appropriate duration for generating proliferative neurospheres and they strongly expressed Nestin, an NSPC marker. Neurosphere reformation after being dissociated by the Accutase enzyme was significantly higher than other methods. Passage number of DPSCs did not affect neurosphere formation, but did influence neuronal differentiation. We found that the cells expressing a neuronal marker, β-tubulin III, and exhibiting neuronal morphology were significantly higher in the early passage of the DPSCs. Conclusion These results suggest a guideline to obtain a high efficiency of neurospheres and neuronal differentiation from DPSCs for further study and neurodegeneration therapeutics.
Primary Thrombophilia XVI: A Look at the Genotype of the Sticky Platelet Syndrome Phenotype

The sticky platelet syndrome (SPS) was described by Mammen in 1983. Since then, scientists in several countries have identified the condition and published cases or series of patients, thus enabling the description of the prevalence of the inherited condition, its salient clinical features, and the treatment of the disease. The diagnosis of the SPS phenotype requires fresh blood samples and special equipment which is not available in all coagulation laboratories. In the era of molecular biology, up to now it has not been possible to define a clear association of the SPS phenotype with a specific molecular marker. Some molecular changes which have been described in platelet proteins in some persons with the phenotype of the SPS are here discussed. Nowadays, the SPS phenotype may be considered as a risk factor for thrombosis and most cases of the SPS developing vaso-occlussive episodes are the result of its coexistence with other thrombosis-prone conditions, some of the inherited and some of them acquired, thus leading to the concept of multifactorial thrombophilia. Ignoring all these evidence-based concepts is inappropriate, same as stating that the SPS is a nonentity simply because not all laboratories are endowed with adequate equipment to support the diagnosis.
Safety of Japanese encephalitis vaccines

ABSTRACT Japanese encephalitis (JE) is an endemic disease dominantly in the Asia-Pacific region with mortality rate varying between 3% and 30%. Long-term neuropsychiatric sequelae developed in 30–50% of the survivors. There is no available antiviral therapy for JE. JE vaccines play a major role in preventing this devastating disease. The incidence of JE declined over years and the age distribution shifted toward adults in countries where JE immunization program exists. Mouse brain–JE vaccine is currently replaced by inactivated Vero cell-derived vaccine and live-attenuated vaccine using SA14-14-2 strain, and live chimeric JE vaccines. These three types of JE vaccines are associated with favorable efficacy and safety profiles. Common adverse reactions include injection site reactions and fever, and severe adverse reactions are rare.
Structural and Functional Impact of Damaging Nonsynonymous Single Nucleotide Polymorphisms (nsSNPs) on Human VPS35 Protein Using Computational Approaches

Parkinson's disease is the second most common progressive neurodegenerative movement disorder. Mutations in retromer complex subunit and VPS35 represent the second most common cause of late-onset familial Parkinson's disease. The mutation in VPS35 can disrupt the normal protein functions resulting in Parkinson's disease. The aim of this study was the identification of deleterious missense Single Nucleotide Polymorphisms (nsSNPs) and their structural and functional impact on the VPS35 protein. In this study, several insilico tools were used to identify deleterious and disease-associated nsSNPs. 3D structure of VPS35 protein was constructed through MODELLER 9.2, normalized using FOLDX, and evaluated through RAMPAGE and ERRAT whereas, FOLDX was used for mutagenesis. 25 ligands were obtained from literature and docked using PyRx 0.8 software. Based on the binding affinity, five ligands i.e., PG4, MSE, GOL, EDO, and CAF were further analyzed. Molecular Dynamic simulation analysis was performed using GROMACS 5.1. 4, where temperature, pressure, density, RMSD, RMSF, Rg, and SASA graphs were analyzed. The results showed that the mutations Y67H, R524W, and D620N had a structural and functional impact on the VPS35 protein. The current findings will help in appropriate drug design against the disease caused by these mutations in a large population using in-vitro study.
An updated SYSCILIA gold standard (SCGSv2) of known ciliary genes, revealing the vast progress that has been made in the cilia research field

Cilia are microtubule-based organelles with important functions in motility and sensation. They contribute to a broad spectrum of developmental disorders called ciliopathies and have recently been linked to common conditions such as cancers and congenital heart disease. There has been increasing interest in the biology of cilia and their contribution to disease over the past two decades. In 2013 we published a “Gold Standard” list of genes confirmed to be associated with cilia. This was published as part of the SYSCILIA consortium for systems biology study dissecting the contribution of cilia to human health and disease, and was named the Syscilia Gold Standard (SCGS). Since this publication, interest in cilia and understanding of their functions have continued to grow, and we now present an updated SCGS version 2. This includes an additional 383 genes, more than doubling the size of SCGSv1. We use this dataset to conduct a review of advances in understanding of cilia biology 2013– 2021 and offer perspectives on the future of cilia research. We hope that this continues to be a useful resource for the cilia community.
Joseph John Thomson investigates the paranormal

Joseph John Thomson is best known for detecting two isotopes of neon within cathode ray tubes that lay the foundation of the field of mass spectrometry. He was awarded the 1906 Nobel Prize in Physics for the discovery of the electron and for his work on the conduction of electricity in gases in the same devices. He is less known for his strong religious beliefs and his interest in psychical research and the paranormal. Thomson served as a member of the Society for Psychical Research for over 50 years and even became its Vice President. During this time, he attended a number of séances and demonstrations by professed psychics and mediums. This article traces those who influenced his interest in the paranormal, from Balfour Stewart to Lord Rayleigh and William Crookes. It reports and illustrates his beliefs and experiences investigating the paranormal in his own words.