Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of Intracellular Na in Ca Overload and Depressed Recovery of Ventricular Function of Reperfused Ischemic Rat Hearts

The roles of H-Na and Na-Ca exchange in the depression of ventricular function were studied in the reperfused isolated ischemic rat heart. Zero-flow global ischemia was induced for either IS or 30 minutes and was followed by 30 minutes of aerobic reperfusion. Intracellular Na (Na,) and ^Ca* uptake were measured during ischemia and reperfusion. Accumulation of Na, was modified by prior grycogen depletion and by treatment with amiloride, a H-Na exchange inhibitor, or monensin, a Na ionophore. Na, rose continuously during ischemia and rapidly during the first two minutes of reperfusion. The larger inhibitory effect of amiloride and preischemic glycogen depletion was on Na, accumulation during reperfusion; this finding suggests that the uptake occurs by H-Na exchange. Reduction of Na, accumulation by glycogen depletion was associated with less lactate and, presumably, H production and accumulation during ischemia. The rapid increase in Na, during early reperfusion may reflect the readjustment of the low intracellular pH resulting from ischemia. The level of Na, at the end of ischemia and especially after two minutes of reperfusion were linearly correlated with **Ca uptake and depression of ventricular function during subsequent reperfusion. This highly significant correlation between Na, and ^Ca* uptake when Na| was varied by several independent procedures, including monensin, strongly suggests that reperfiision Ca uptake occurs at least in part by Na-Ca exchange. The rate of Ĉa"" uptake during reperfusion was linearly and highly significantly correlated with elevation of diastolic pressure, reduced developed pressure, and decreased recovery of ventricular function. The data strongly support a mechanism of ischemic cell damage that involves excessive production and accumulation of H during Ischemia that exchanges for extracellular Na during ischemia and rapidly during the first few minutes of reperfusion. Increased Nai then causes excessive "Ca* uptake and depressed recovery of cellular functions with continued reperfusion. Increased levels of Na| may be a major event that couples a decreased intracellular pH during ischemia to excessive ^Ca uptake and depressed recovery of cellular function with reperfusion. (Circulation Research 1989;65:1045-1056)
OTT_A_216161 7685..7690

Zi-Kang Luo* Qiong-Feng Chen* Xiaoqin Qu Xiao-Yan Zhou 1Department of Clinical Medical, The Second Clinical Medical College, Nanchang University, Nanchang 330006, People’s Republic of China; 2Department of Pathophysiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 3Jiangxi Province Key Laboratory of Tumor Pathogenesis and Molecular Pathology, Nanchang, Jiangxi 330006, People’s Republic of China
Chronic Kidney Disease

A fact well known to the readers of the Journal: heart disease is the number 1 killer in our society, contributing to 600,000 deaths annually (1). Coronary heart disease (CHD) is responsible for approximately two-thirds of these deaths, and 935,000 Americans experiencemyocardial infarction (MI) each year (1). Hypertension, elevated low-density lipoprotein cholesterol levels, smoking, and diabetes arewell appreciated as risk factors. However, a particularly ominous risk for CHD arises in the setting of chronic kidney disease (CKD) (2). As renal function declines belowaglomerularfiltration rate of 60ml/min/1.73m, the relative risk for cardiovascular mortality progressively increases by 3-fold, as compared with those without CKD (3), and almost 10-fold in the setting of CKD with diabetes (4). Indeed, the cardiovascular risk conveyed by CKD exceeds that conveyed by diabetes (5,6).
Clustering For Mining a Product Purchasing or verifying Online

- Nowadays, a giant a part of individuals have confidence available content in social media in their choices (e.g. reviews and feedback on a subject or product). the likelihood that anybody will leave a review offer a golden chance for spammers to write down spam reviews concerning product and services for various interests. Identifying these spammers and also the spam content may be a hot topic of analysis and though a substantial variety of studies have been done recently toward this finish, however thus far the methodologies put forth still barely find spam reviews, and none of them show the importance of every extracted feature sort. In this study, we propose a unique framework that utilizes spam options for modeling review datasets as heterogeneous information networks to map spam detection procedure into a classification downside in such networks. mistreatment the importance of spam options facilitate the United States to get higher leads to terms of different metrics experimented on real-world review datasets from Yelp and Amazon websites. The results show that our project outperforms the present strategies and among four classes of features; together with review-behavioral, user-behavioral, review linguistic, user-linguistic, the primary form of options performs higher than the opposite classes.
Fundamental Concepts in the Embryogenesis of Dicotyledons: A Morphological lnterpretation of Embryo Mutants

Embryogenesis represents the critical stage in the development of a plant at which its basic organization and body plan have their inception. Therefore, in the effort to understand the regulation of plant development, molecular geneticists have recently focused considerable attention on the search for genes expressed during the embryogenesis of plant model systems (Mayer et al., 1991 ; Meinke, 1991 b; Lindsey and Topping, 1993; West and Harada, 1993; Goldberg et al., 1994). This molecular approach toward plant embryogenesis has been given even greater impetus by the significant advances made in the isolation of embryonic genes in animal model systems, such as in Drosophila (Nüsslein-Volhard and Wieschaus, 1980; Nüsslein-Volhard, 1991) and zebrafish (Kane et al., 1996; Solnica-Krezel et al., 1996). As a consequence of these noteworthy successes in animal embryogenesis, there has been a natural tendency for plant biologists to follow suit and interpret molecular genetic data in terms of animal developmental models (Jürgens et al., 1991 ; Mayer et al., 1991). One problem arising from this parallel strategy toward the study of plant and animal embryogenesis is that plant embryos bear a significantly different relationship to their adult forms than do animal embryos. This situation is further complicated by the history of the field of plant embryology, in which certain perspectives have led to less fundamental, even ambiguous characterizations of embryo morphogenesis. For example, traditional studies of plant embryogenesis have placed great emphasis on detailed characterizations of cell lineages in the earliest stages of plant embryogenesis (Johansen, 1950; Natesh and Rau, 1984). This kind of emphasis was usually at the expense of the more general features of embryo morphogenesis, such as the time at which the meristems of the shoot and root are first organized and begin to function. We demonstrate that it is the morphology of the embryo that determines the cell lineage patterns rather than vice versa.
University of Birmingham Molecular characterisation of the vacuolating autotransporter toxin in uropathogenic escherichia coli

The vacuolating autotransporter (AT) toxin (Vat) contributes to Uropathogenic Escherichia coli 31 (UPEC) fitness during systemic infection. Here we characterised Vat and investigated its 32 regulation in UPEC . We assessed the prevalence of vat in a collection of 45 UPEC urosepsis 33 strains and showed that it was present in 31 (68%) of the isolates. The isolates containing the vat 34 gene corresponded to three major E. coli sequence types (ST12, 73 and 95) and these strains 35 secreted the Vat protein. Further analysis of the vat genomic locus identified a conserved gene 36 located directly downstream of vat that encodes a putative MarR-like transcriptional regulator, 37 which we termed vatX . The vat-vatX genes were present in the UPEC reference strain CFT073 38 and RT-PCR revealed both genes are co-transcribed. Over-expression of vatX in CFT073 led to a 39 3-fold increase in vat gene transcription. The vat promoter region contained three putative 40 nucleation sites for the global transcriptional regulator H-NS; thus the hns gene was mutated in 41 CFT073 (to generate CFT073 hns ). Western blot analysis using a Vat-specific antibody revealed 42 a significant increase in Vat expression in CFT073 hns compared to wild-type CFT073. Direct H- 43 NS binding to the vat promoter region was demonstrated using purified H-NS in combination 44 with electrophoresis mobility shift assays. Finally, Vat-specific antibodies were detected in 45 plasma samples from urosepsis patients infected by vat -containing UPEC strains, demonstrating 46 Vat is expressed during infection. Overall, this study has demonstrated that Vat is a highly 47 prevalent and tightly regulated immunogenic SPATE secreted by UPEC during infection.
Time to Change Our Focus

Ma Despite numerous groundbreaking advances in thefield, cardiovascular disease remains the leading cause ofmortality in the United States, accounting for more than 787,000 deaths per year. Already leading the world in per capita healthcare expenditure, U.S. medical costs related to cardiovascular disease are projected to triple by 2030, to over $800 billion annually. The medical community’s traditional disproportionate focus on treating cardiovascular disease, relative to promoting cardiovascular health, is an important contributor to these expenses. To ensure continued reductions in the burden of cardiovascular disease, as well as the overall sustainability of the healthcare system, a paradigm shift that places more emphasis on cardiovascular health promotion throughout the life course is required. This review will discuss the current definitions of cardiovascular health, as well as strategies for promoting and impacting cardiovascular health at both the local and national level. (J Am Coll Cardiol 2015;66:960–71) © 2015 by the American College of Cardiology Foundation.
Transport of NH 3 / NH 4 1 in oocytes expressing aquaporin-1

Nakhoul, Nazih L. , Kathleen S. Hering-Smith, Solange M. Abdulnour-Nakhoul, and L. Lee Hamm. Transport of NH3/NH4 1 in oocytes expressing aquaporin-1. Am J Physiol Renal Physiol 281: F255–F263, 2001. —The aim of this study was to determine whether expressing aquaporin (AQP)-1 could affect transport of NH3. Using ion-selective microelectrodes, the experiments were conducted on frog oocytes (cells characterized by low NH3 permeability) expressing AQP1. In H2O-injected oocytes, exposure to NH3/ NH4 1 (20 mM, pH 7.5) caused a sustained cell acidification and no initial increase in pHi (as expected from NH3 influx), and the cell depolarized to near 0 mV. The absence of Na, the presence of Ba21, or raising bath pH (pHB) did not inhibit the magnitude of the pHi decrease or result in an initial increase in pHi when NH3/NH4 1 was added. However, after the cell was acidified (because of NH3/NH4 ), raising pHB to 8.0 caused a slow increase in pHi but had no effect on membrane potential. The changes in pHi with raising pHB did not occur in the absence of NH3/NH4 . In AQP1 oocytes, exposure to NH3/NH4 1 usually resulted in little or no change in pHi, and in the absence of Na there was a small increase in pHi (the cell still depolarized to near 0 mV). However, after exposure to NH3/NH4 , raising pHB to 8.0 caused pHi to increase more than two times faster than in control oocytes. This increase in pHi is likely the result of increased NH3 entry and not the result of NH4 1 transport. These results indicate that 1) the oocyte membrane, although highly permeable to NH4 , has a significant NH3 permeability and 2) NH3 permeability is enhanced by AQP1.
Modulates Basal Lymphocyte Motility in the Class IA Phosphoinositide 3-Kinase

Recruitment of PI3K to the cell membrane is an indispensable step in normal lymphocyte proliferation and activation. In this study we identify PI3K as an important signaling molecule for maintaining basal T and B lymphocyte motility and homing in the intact lymph node. Pharmacological inhibition of PI3K catalytic isoforms exerted broad effects on basal lymphocyte motility, including changes in homing kinetics, localization of B cells within the lymph node, and reduced cell velocities. Lymphocytes deﬁcient in either or both of the class IA PI3K regulatory subunits p85 (cid:1) and p85 (cid:2) also exhibited reduced velocities, with the magnitude of reduction depending upon both cell type and isoform speciﬁcity. B cells deﬁcient in p85 (cid:1) exhibited gross morphological abnormalities that were not evident in cells treated with a PI3K inhibitor. Our results show, for the ﬁrst time, that class IA PI3Ks play an important role in regulating basal lymphocyte motility and that p85 (cid:1) regulatory subunit expression is required to maintain B cell morphology in a manner independent of PI3K catalytic function. Moreover, we demonstrate distinct roles for catalytic domain function and class IA PI3K regulatory domain activity in lymphocyte motility, homing, and homeostatic localization of mature resting B cells. The Journal of Immunology, 2007, 179: 2261–2269. 129SvEv) and were studied at 2–4 mo of age. Wild-type F 1 C57BL/6 (cid:3) 129SvEv mice were used as control animals. We found no differences in T cell velocity in adoptively transferred C57BL/6 (cid:3) 129SvEv cells in littermate recipients, when compared with C57BL/6 transferred into C57BL/6, as late as 8 h after adoptive transfer (data not shown). Animals were housed in autoclaved microiso-lator cages with sterilized food and water. All procedures were ap- proved by the University of California Irvine Institutional Animal Care and Use Committee.
Prevalence of determinants among extended-spectrum -lactamase-positive Enterobacteriaceae clinical isolates in southern Stockholm, Sweden

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Prevalence of determinants among extended-spectrum β-lactamase-positive Enterobacteriaceae clinical isolates in southern Stockholm, Sweden Hong Fang, Haihui Huang, Yuejie Shi, Göran Hedin, Carl Erik Nord, Måns Ullberg
The Pragmatic School of Thought in Open Science: A Case Study of Multi-stakeholder Participation in Shaping the Future of Internet Governance

The internet is a disruptive technology that continues to define our modern world. However, numerous ethical challenges remain for internet governance going forward, e.g. surveillance capitalism, terrorism and radicalisation. The ‘pragmatic’ school of thought in open science advocates for collaboration between diverse stakeholder groups (e.g. citizens, academics, practitioners, policymakers) to ensure an informed, and positive imprint for change. However, our understanding of how open science can be used for assimilating knowledge on complex socio-political issues remains nascent. To address this gap, we present findings from ‘We, the Internet’, a global consultation project which utilised open science practices such as stakeholder-led evaluations and open access publications to engage stakeholders in dialogue around the future of internet governance. Our findings discuss emergent themes on the future of internet governance, and highlight the potential of open science to mobilise groups and combat public scepticism in policy-making.
The Design of a Web Contents Management System

According to analysis at SNL Kagan, the market share of IPTV is rapidly growing whereas the market share of cable TV is slowly shrinking. This paper introduces an ‘Open IPTV System’ which allows a subscriber to easily open his or her own IPTV broadcasting station. In the ‘Open IPTV System’, operators must be present at the office to access the content management system (CMS). We implement a web CMS so that the operators can access the CMS anytime and anywhere as long as they are connected to the Internet.
A Fuzzy-Genetic Based Approach for Improving OLSR Routing Protocol in Mobile Adhoc Networks

: One of the MANET routing protocol, Optimized Link State Routing (OLSR) is based on link state routing protocol. In OLSR, the flooding of packets is optimized by using multipoint relay (MPR) nodes. The MPR nodes have the responsibility for controlling and forwarding traffic in the network. The efficiency of the OLSR protocol is based on the selected MPRs. So, it is essential to select the nodes possessing certain quality as MPR nodes in the network. In this paper, MPR selection is achieved using Fuzzy Logic based on the metrics lifetime, stability and buffer limit of the nodes. Then, the path to be established from source to destination through the selected MPRs is optimized using genetic algorithm. By using quality MPR, the efficiency of the OLSR routing protocol is improved and is verified by simulation.
Orthogonally polarized frequency combs in a mode-locked VECSEL

1Weierstrass Institute, Mohrenstrasse 39, 10117 Berlin, Germany 2Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics (B-PHOT), Pleinlaan 2, B-1050 Brussels, Belgium 3Institute of Solid State Physics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee Boulevard, 1784 Sofia, Bulgaria 4Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du triomphe, 1050 Brussels, Belgium *Corresponding author: kpanajot@b-phot.org
Anaerobic co – digestion of pig and poultry droppings with elephant grass for the production of biogas

Co – digestion of elephant grass, poultry and pig droppings anaerobically for laboratory scale production of biogas was under taken. The pH and temperature ranges for this study were 5.5 – 7.1 and 25C – 30C respectively within the hydraulic retention time of 52 days. 9.10% total solid concentration was used in each of the digesters. The water displacement method was used to estimate the biogas produced. The percentage weight ratio distribution of poultry droppings to pig droppings were; (100:0), (75:25), (50:50), (25:75), and (0:100) for digesters A, B, C, D and E respectively. Digester B gave the maximum biogas yields of 301 cmCH4/ g – VSadded at the end of 52 days of fermentation after which there was no further production. It is suggested that the presence of polycyclic aromatic hydrocarbon, alkanes, SP and methyl functional group in all these substrates used as shown by the Fourier transform infrared spectroscopy carried out make these materials be good for biogas production. The GC analysis on the biogas produced in digester B had maximum production showed 69.43 %v/v and 23.22 %v/v for methane (CH4) and carbon dioxide (CO2) respectively. The experimental data fitted well with the linear kinetic model which indicated that there was an increase in the yield of biogas as the retention time increases. The net performance of the digesters were, digester B > digester C > digester A > digester D > digester E. X – RF analysis carried out on the substrates showed that poultry dropping has more Fe2O3, CaO, P2O5, K2O, and Mn2O3 essential elements required for enzymes and microbial metabolisms in anaerobic digestion. This makes poultry droppings a very viable substrate for biogas production compared to the other two substrates. The overall power generations were 6.54, 9.57, 7.8, 5.4, and 1.89 watt in digesters A, B, C, D, and E respectively.
The Biotrophic Development of Ustilago maydis Studied by RNAseq Analysis

1Max-Planck-Institut für Terrestrische Mikrobiologie, Abt. Organismische Interaktionen, Karl-vonFrisch Straße 10, 35043 Marburg, Germany 2Philipps Universität Marburg, Fb17 Biologie, AG Zellbiologie der Pflanzen, Karl-von-FrischStraße 8, 35043 Marburg, Germany 3 Cologne Center for Genomics (CCG), University of Cologne, Weyertal 115b, 50931 Cologne, Germany. 4 Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany. 5 BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany a Present address: Bayer CropScience Biologics GmbH, Metkenberg 6, 23970 Wismar, Germany b Present address: RWTH Aachen University, BIO3: Plant Physiology, Plant Biochemistry and Molecular Biology, Worringer Weg 1, 52074 Aachen, Germany * These authors contributed equally to this work
Gamification of Educational Technology: a Narrative Review

Student motivation and engagement are major problems in education. Gamification is one way to increase student motivation and engagement by combining game element in the educational environment. There are some empirical researches and review paper that has been conducted to give an evidence of gamification positive effect for educational technology but review papers that discuss practical suggestion have rarely been studied. This review is focused on papers that give an empirical evidence for gamification on learning in the context of narrative analysis to proposed best practices to do this research field. The study of gamification in educational technology is the basis this narrative literature review was made. Terms of search used is gamification, learning, student, engagement, motivation, and education. Gamification on e-learning that has no purpose on student motivation and engagement were excluded from the search terms. Fifteen papers were selected for review as they are relevant to the criteria. This review indicates that the use of game element has a positive influence on educational technology for the student, even there are various combinations for the chosen of learning activity, subject, game elements, method, sample size, and duration.
Contribution of Biofertilizers: Towards sustainable agriculture

: Agricultural workers are increasingly turning to organic farming practices to balance crop input and output economics, while becoming increasingly conscious of environmental changes due to climate change. Use of eco-friendly, bio-pesticides, bio-fertilizer and bio-controls is being encouraged in the field of agriculture. Bio-pesticides like neem and Bacillus -based pesticides such as Aureofungin, Kasugamycin, Validamycin, Streptomycin and Sulphate and Tetracycline Hydrochloride have been identified for controlling various insect pests and diseases in agriculture.
enza: immunoprophylaxis with inactivated (killed-virus) vaccine and chemoprophylaxis or therapy with influenza-specific antiviral Une déclaration d’un comité consultatif (DCC) Comité consultatif national de l’immunisation (CCNI) DÉCLARATION SUR LA VACCINATION ANTIGRIPPALE

The National Advisory Committee on Immunization (NACI) provides Health Canada with ongoing and timely medical, scientific, and public health advice relating to immunization. Health Canada acknowledges that the advice and recommendations set out in this statement are based upon the best current available scientific knowledge, and is disseminating this document for information purposes. Persons administering or using the vaccines or drugs discussed in this statement should also be aware of the contents of the relevant product monograph(s). Recommendations for use and other information set out here may differ from that set out in the product monograph(s) of the Canadian licensed manufacturer(s) of the vaccine(s). Manufacturer(s) have sought approval of the vaccine(s) and provided evidence as to its safety and efficacy only when used in accordance with the product monographs. NACI members and liaison members conduct themselves within the context of Health Canada’s Policy on Conflict of Interest, including yearly declaration of potential conflict of interests.