Miracle-dz/UniFAR-FTUs huggingface dataset:
siRNA Targeting Mcl-1 Potentiates the Anticancer Activity of Andrographolide Nanosuspensions via Apoptosis in Breast Cancer Cells

Breast cancer is the second leading cause of cancer-related death in the US. However, recurrence is frequently found despite adjuvant therapy being available. Combination therapy with cytotoxic drugs and gene therapy is being developed to be a new promising cancer treatment strategy. Introducing substituted dithiocarbamate moieties at the C12 position of andrographolide (3nAG) could improve its anticancer selectivity in the MCF-7 breast cancer cell line. However, its hydrophobicity is one of its main drawbacks. This work successfully prepared 3nAG nanosuspension stabilized with the chitosan derivative NSC (3nAGN-NSC) to increase solubility and pharmacological effectiveness. siRNAs have emerged as a promising therapeutic alternative for interfering with particular mRNA. The 3nAGN-NSC had also induced Mcl-1 mRNA expression in MCF-7 human breast cancer cells at 8, 12, and 24 h. This indicates that, in addition to Mcl-1 silencing by siRNA (siMcl-1) in MCF-7 with substantial Mcl-1 reliance, rationally devised combination treatment may cause the death of cancer cells in breast cancer. The Fa-CI analysis showed that the combination of 3nAGN-NSC and siMcl-1 had a synergistic effect with a combination index (CI) value of 0.75 (CI < 1 indicating synergistic effects) at the fractional inhibition of Fa 0.7. The synergistic effect was validated by flow cytometry, with the induction of apoptosis as the mechanism of reduced cell viability. Our findings suggested the rational use of 3nAGN-NSC in combination with siMcl-1 to kill breast cancer cells.
AAPM Task Group Report 303 endorsed by the ABS: MRI Implementation in HDR Brachytherapy-Considerations from Simulation to Treatment.

The Task Group (TG) on Magnetic Resonance Imaging (MRI) Implementation in High Dose Rate (HDR) Brachytherapy - Considerations from Simulation to Treatment, TG 303, was constituted by the American Association of Physicists in Medicine's (AAPM's) Science Council under the direction of the Therapy Physics Committee, the Brachytherapy Subcommittee, and the Working Group on Brachytherapy Clinical Applications. The TG was charged with developing recommendations for commissioning, clinical implementation, and on-going quality assurance (QA). Additionally, the TG was charged with describing HDR brachytherapy (BT) workflows and evaluating practical consideration that arise when implementing MR imaging. For brevity, the report is focused on the treatment of gynecologic and prostate cancer. The TG report provides an introduction and rationale for MRI implementation in BT, a review of previous publications on topics including available applicators, clinical trials, previously published BT related TG reports, and new image guided recommendations beyond CT based practices. The report describes MRI protocols and methodologies, including recommendations for the clinical implementation and logical considerations for MR imaging for HDR BT. Given the evolution from prescriptive to risk-based QA,1 an example of a risk-based analysis using MRI-based, prostate HDR BT is presented. In summary, the TG report is intended to provide clear and comprehensive guidelines and recommendations for commissioning, clinical implementation, and QA for MRI-based HDR BT that may be utilized by the medical physics community to streamline this process. This report is endorsed by the American Brachytherapy Society (ABS). This article is protected by copyright. All rights reserved.
Mechanical Properties and Electrical Resistivity of the Friction Stir Spot-Welded Dissimilar Al–Cu Joints

Alternative methods for dissimilar metal joining particularly aluminium and copper have gain interest in manufacturing sectors. Friction stir spot welding was carried out on the AA6061 and C11000 wires of 2 mm diameter. This research paper reported the results on microstructures and mechanical properties of the spot-welded joints, and also special attention is provided for electrical resistivity of the welds. The microstructures reveal the information of grain structure and bonding. The width of diffusion layer significantly reduced with low dwell times. For a plunge depth of 1 mm, the maximum tensile strength (294 MPa) is achieved during the higher rotational speed (1400 rpm). For the same plunge depth, lower tensile strength values are exhibited by the joint produced using the lower rotational speed (800 rpm). Hardness of the weld region recorded 70 HV which is less than Cu (115 HV) and greater than Al (40 HV). FSSW joints (0.30 to 0.34 μΩ) offered higher range of electrical resistivity than that of base metal (0.02 μΩ). The results highlighted in this paper might be helpful for both academic researchers and industrialists.
Containers checkpointing and live migration

Container-type virtualization is an ability to run multiple isolated sets of processes, known as containers, under a single kernel instance. Having such an isolation opens the possibility to save the complete state of (in other words, to checkpoint) a container and later to restart it. Checkpointing itself is used for live migration, in particular for implementing high-availability solutions. In this paper, we present the checkpointing and restart feature for containers as implemented in OpenVZ. The feature allows one to checkpoint the state of a running container and restart it later on the same or a different host, in a way transparent for running applications and network connections. Checkpointing and restart are implemented as loadable kernel modules plus a set of userspace utilities. Its efficiency is proven on various realworld applications. The overall design, implementation details and complexities, and possible future improvements are explained.
Controllable III–V nanowire growth via catalyst epitaxy

Controllable synthesis of III–V compound semiconductor nanowires (NWs) with high crystallinity and uniformity is essential for their large-scale practical use in various technological applications, especially for those which are grown on non-crystalline substrates. In this study, the catalytic effect is investigated thoroughly in the growth of various III–V NWs in solid-source chemical vapor deposition, including Pd, Ag and Ni catalyzed GaAs NWs and Au catalyzed InGaAs and GaSb NWs. It is found that small diameter catalyst seeds lead to faster NW growth with better crystal quality, while large seeds result in slower NW growth with kinked morphology and twinning defects. Importantly, these small catalyst nanoparticles are observed to have higher solubility of the group III precursors due to the Gibbs–Thomson effect, which not only enables effective III precursor diffusion for a faster growth rate, but also yields epitaxial growth of NWs from the catalyst seeds accounting for the low activation energy and better crystallinity. All these results explicitly demonstrate the effectiveness of this catalyst solubility and epitaxy engineering for controlled III–V NW growth and indicate the potency for the reliable production of high-performance NWs for next-generation electronics.
Development of an optical system for the SST-1M telescope of the Cherenkov Telescope Array observatory

The prototype of a Davies-Cotton small size telescope (SST-1M) has been designed and developed by a consortium of Polish and Swiss institutions and proposed for the Cherenkov Telescope Array (CTA) observatory. The main purpose of the optical system is to focus the Cherenkov light emitted by extensive air showers in the atmosphere onto the focal plane detectors. The main component of the system is a dish consisting of 18 hexagonal mirrors with a total effective collection area of 6.47 m 2 (including the shadowing and estimated mirror reflectivity). Such a solution was chosen taking into account the analysis of the Cherenkov light propagation and based on optical simulations. The proper curvature and stability of the dish is ensured by the mirror alignment system and the isostatic interface to the telescope structure. Here we present the design of the optical subsystem together with the performance measurements of its components.
Cyclin-dependent Rimise Inhibitor p57KIP2in Soft Tissue Sarcomas and Wilms' Tumors1

Mammalian cyclin-dependent kinase inhibitors fall into two families, the INK4 and the CIP/KIP. The CIP/KIP family comprises three struc turally related members, including V2\CÃŒP"WAF', p21K'rl, and p57*7". These proteins are all capable of inhibiting the progression of the cell cycle by binding and inhibiting G, cyclin/cyclin-dependent kinase complexes. In humans, pS7K"" is expressed specifically in skeletal muscle, heart, brain, kidney, and lung. Human KIP2 resides in llplS.5, a chromosomal region that is a common site for loss of heterozygosity in certain sarcomas, Wilms' tumors, and tumors associated with the Beckwith-Wiedemann syndrome. Because of the function, selective expression, and chromosomal location of p57A"'2, we undertook the present study to search for potential mutations of KIP2 in a cohort of 126 tumors composed of 75 soft tissue sarcomas and 51 Wilms' tumors. The KIP2 gene was characterized by Southern blot, comparative multiplex PCR, PCR-single-strand conformational polymorphism, and DNA sequencing assays in these neoplasms. Deletions of the KIP2 gene or point mutations at the region encoding the cyclin-dependent kinase inhibitory domain were not found in the tumors analyzed. The absence of KIP2 mutations might indicate that these tumors arise due to defects at a closely linked but separate locus. Alternatively, similarly to the mouse homologue, inactivation of K1P2 could occur via genomic imprinting.
C4 Acid Metabolism and Dark CO2 Fixation in a Submersed Aquatic Macrophyte (Hydrila verticillata)'

The CO0 compensation point of the submersed aquatic macrophyte HydriAW vertkiclata varied from bigh (above 50 microlters per liter) to low (10 to 25 microliters per liter) values, depnding on the growth conditions. Plants from the lake in winter or after incubation in an 11 C/9-hour photoperlod had higb vahls, whereas summer plants or those incubated in a 27 C/14-hour photoperiod had Ow Values The plants With lOw CO, comensation points exhibited dark '4C0, fixation rates that were up to 30% of the light fixation rates. This fixtion reduced respiratory CO, loss, but did not result in a net uptake of CO, at night. Tbe low compensation point plants also showed diunal fluctuations in titratable acid, such as occur in Crassulacea acid metaboism plants. However, dark fixation and diurnal acid fluctuations were n le in Hydria plants with high CO, compensation points. Exposure of the low compensation point plants to 20 micromolar A4C02 resulted in 60% of the 14C being incorporated into maate and aspartte, with only 16% in sugar phospates. At a high CO level, the C4 acid label was decreased. A pulse.chase study indicated that the 14C in malate, but not aspartate, decreased after a long (270-second) chase period; thus, the C4 acid turnover was much slower than in C4 plants.
CHAPTER 7: SEMICONDUCTOR PHOTOCATHODES FOR UNPOLARIZED ELECTRON BEAMS

Semiconductor photocathodes offer great advantage for generating high average currents by the virtue of their high quantum efficiency in the UV or VIS spectral ranges. This chapter reviews basic properties of these photoemissive materials. Standard growth and preparation procedures for antimony and tellurium based alkali photocathodes as well as for III-V semiconductors activated to negative electron affinity are presented. Various technical requirements and instrumentation needed either for the synthesis or the activation of these materials are outlined. 7.1 PHOTOCATHODE TYPES High quantum efficiency photocathodes are desirable for generating high average (greater than milliamperes) beam current. Essentially, all such photocathodes are semiconductors. Two general types of semiconductor photocathodes used in photoinjectors producing unpolarized electron beams are alkali-based ones (antimonides and tellurides), and bulkor layered-III-V crystals (e.g., GaAs or GaN) activated to the condition of negative electron affinity via the deposition of roughly a monolayer of cesium on the surface. In this chapter, we detail the important characteristics of these photocathodes, their growth procedures, and their performance in the accelerator environment. 7.2 DEFINITIONS AND PHOTOCATHODES’ FIGURES OF MERIT The intrinsic properties of the photocathode play a key role in establishing the quality of the electron beam (its emittance and time structure), the reliability of its operation, and the ease of maintenance. An Engineering Guide to Photoinjectors, T. Rao and D. H. Dowell, Eds. Chapter 7: Semiconductor Photocathodes for Unpolarized Electron Beams, I. Bazarov, L. Cultrera and T. Rao 185 The most relevant properties for a photocathode inside a photoinjector are its quantum efficiency and its spectral response, the photoemission response time, the thermal emittance (or intrinsic emittance), mean transverse energy, and the lifetime of the photocathode. Also of importance is its ability to withstand an adverse environment inside the photoemission gun, such as the back-streaming of ions, created via ionizing residual gas by the primary electron beam, towards the cathode. Its lifetime can also be negatively affected by residual gas inside the gun chamber. These effects determine the vacuum requirements for the photocathode (sensitivity to the partial pressure of various gasses encountered in ultra-high vacuum (UHV) systems) to maintain a high QE. Here, we summarize the main definitions used in this chapter. Quantum efficiency (QE) is defined as the number of electrons per incident photon, given by the formula
Research On The Heat Transfer Law Of Gas-liquidTwo-phase Flow In A Deepwater Wellbore

According to the features of low temperature and Type III boundary conditions during deepwater oil and gas exploitation, an experimental system was designed and built to simulate the heat transfer of deepwater oil and gas. A large number of laboratory experiments were carried out and the relational expressions of the heat transfer as well as the influence of the gas-liquid discharge on the heat transfer of each gas-liquid two-phase flow patterns under the deepwater and lowtemperature Type III boundary conditions were obtained. The results show that the expression in the laminar flow obtained in this paper is largely different from the relational expression of the heat transfer at ordinary temperatures under the simple boundary conditions. The two expressions are in substantial agreement in the turbulent flow. The liquid discharge is the principal factor which influences the heat transfer in the bubbly flow, slug flow and annular flow while the gasliquid two-phase flow has little influence on the heat transfer in the churn flow.
A Review of the Energy Efficiency Improvement in DC Railway Systems

This study is focused on the topical issue of increasing the energy efficiency in DC railway systems, in the context of global concerns for reducing the CO2 emissions by minimizing the energy consumption and energy loss. The main achievements in this complex issue are synthesized and discussed in a comprehensive review, emphasizing the implementation and application of the existing solutions on concrete case studies. Thus, all specific subtopics related to the energy efficiency are covered, starting with power quality conditioning and continuing with the recovery of braking energy, of which a large part is lost in the classic DC-traction substations. The solutions of onboard and wayside storage systems for the braking energy are discussed and compared, and practical examples are given. Then, the achievements in transforming the existing DC-traction substations in reversible substations with capabilities of power quality improvement are systematically reviewed by illustrating the main results of recent research on this topic. They include the equipment available on the market and solutions validated through implementations on experimental models. Through the results of this extensive review, useful reference and support are provided for the research and development focused on energy efficient traction systems.
FORMULATION AND EVALUATION OF NOVEL DRUG DELIVERY SYSTEM FOR PONGAMIA PINNATA SEED EXTRACT

Purpose: Liposomes have been used to improve the therapeutic index of new or established drugs by modifying drug absorption, reducing metabolism, prolonging biological half-life or reducing toxicity. Liposomal formulations have also been successfully used in the treatment of a number of dermatological diseases. Various synthetic as well as herbal drugs have been incorporated into liposomes to improve its efficacy. Herbal extracts can be incorporated into liposomes in order to increase the drug permeability. Karanja (Pongamia pinnata) seed extract is well known by anti-inflammatory activity. Pongamia pinnata contains flavonoids like karanjin and Pongamol that have excellent antioxidant and photoprotective and anti-inflammatory activity. It also helps to prevent wrinkles, premature skin aging and skin cancer. Karanjin is main constituent in Pongamia pinatta seed which have less absorption through skin; hence there is a need to formulate a novel form in order to increase its absorption through skin. This can be achieved by developing a transdermal drug delivery system. The aim of this study was to formulate effective and stable liposomes as a novel drug delivery system for Pongamia pinatta seed extracts and to evaluate its physico-chemical properties & anti-inflammatory activity. Liposome’s were prepared by incorporating different amount of ingredients together (given below) and a certain amount of the herbal extract. Hence, this extract was incorporated into liposomes to enhance its activity in transdermal delivery. Drug diffusion study was performed to determine the percent release of drug through skin. The objective of the present research work is to convert this age old miraculous herb into nanotechnology based formulations i.e. liposome. Also, formulated liposomes were evaluated for particle size, Zeta potential, encapsulation efficiency, Transmission electron microscopy, In-vitro drug diffusion study, Membrane stabilization study and determination of active constituents by HPTLC. 3, 4 Methods: Pongamia pinnata seed Extract was incorporated into liposomes by thin film hydration method. The batch having lipid ratio i.e. Soya lecithin: Cholesterol (4:1); Pongamia pinnata seed extract concentration 250mg with entrapment efficiency 78% was finalized.
Analisis Sentimen terhadap Penggunaan Aplikasi MySAPK BKN di Google Play Store

Intisari—Dalam mewujudkan kebijakan Sistem Pemerintahan Berbasis Elektronik (SPBE) dan Satu Data ASN di Indonesia, Badan Kepegawaian Negara (BKN) sebagai instansi pemerintah pembina Aparatur Sipil Negara (ASN) perlu melakukan pengelolaan data secara akurat, terkini, dan terintegrasi melalui aplikasi MySAPK berbasis Android. Seiring berjalannya waktu, pengguna mengalami berbagai kendala dalam menggunakan aplikasi dan menuliskan ulasannya pada fitur Rating & Review di Google Play Store. Dalam rentang waktu 9 Mei 2017 hingga 18 Oktober 2021, terdapat 4.778 ulasan yang dituliskan oleh pengguna. Makalah ini menganalisis sentimen pada ulasan pengguna aplikasi MySAPK. Tahapan-tahapan yang dilakukan dimulai dari pengumpulan data, pelabelan data (anotasi), prapemrosesan data, ekstraksi fitur kata, pemodelan klasifikasi, evaluasi pemodelan, analisis sentimen, hingga penyusunan hasil rekomendasi. Dari hasil pemodelan klasifikasi sentimen menggunakan naïve Bayes dan support vector machine (SVM), didapatkan tingkat akurasi masing-masing sebesar 92,47% dan 94,14%. Hasil pengukuran sentimen menunjukkan bahwa pengguna memberikan ulasan bersentimen positif sebanyak 2.118 (44,3%) dan ulasan bersentimen negatif sebanyak 2.660 (55,7%). Di antara faktor penyebab pengguna menuliskan ulasan bersentimen positif adalah aplikasi yang sangat bagus, dapat memberikan manfaat, mempermudah dalam mengisi dan menyimpan data ASN, serta ucapan terima kasih kepada BKN. Sebaliknya, faktor penyebab pengguna menuliskan ulasan bersentimen negatif di antaranya adalah meminta tolong agar aplikasi diperbaiki, kesulitan dalam mengakses, gagal dalam mengisi dan melakukan pemutakhiran data, serta terjadi kesalahan pada server. Untuk mengatasi kendala tersebut, makalah ini merekomendasikan agar kapasitas server pendukung dapat ditingkatkan dan pemutakhiran versi terbaru diluncurkan untuk memperbaiki bug aplikasi. Hasil penelitian ini diharapkan dapat menjadi acuan BKN dalam mengevaluasi dan meningkatkan kualitas pelayanan ASN melalui aplikasi MySAPK.
Towards a better description of cardiovascular function in pulmonary hypertension

Recently, it has been shown that proportional relationships exist between systolic, diastolic and mean pulmonary artery pressure ( P sys , P dia and P mean ) and that they are maintained under various conditions in both health and disease. An arterial-ventricular interaction model was used to study the contribution of model parameters to the ratios P sys / P mean , and P dia / P mean . The heart was modeled by a time-varying elastance function, and the arterial system by a three-element windkessel model consisting of peripheral resistance, R p , arterial compliance C a , and pulmonary artery characteristic impedance Z 0 . Baseline model parameters were estimated in control subjects and compared to values estimated in patients with pulmonary hypertension. Results indicate that experimentally derived ratios P sys / P mean and P dia / P mean could be accurately reproduced using our model (1.59 and 0.61 vs. 1.55 and 0.64, respec-tively). Sensitivity analysis showed that the (empirical) constancy of P sys / P mean and P dia / P mean was primarily based on the inverse hyperbolic relation between total vascular resistance ( R T ; calculated as R p + Z 0 ) and C a , (i.e. constant R T C a product). Of the cardiac parameters, only heart rate affected the pressure ratios, but the contribution was small. Therefore, we conclude that proportional relations between systolic, diastolic and mean pulmonary artery pressure result from the constancy of R T C a thus from pulmonary arterial properties, with only little influence of heart rate.
Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p

Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC.