Miracle-dz/UniFAR-FTUs huggingface dataset:
Virtual and “real-life” wall/rock climbing: motor movement comparisons and video gaming pedagogical perceptions

Abstract The purpose of this study was to examine similarites and differences between motion-based video games (MBVGs) and “real-life” wall/rock climbing and determine the perceived usefulness of utilizing MBVGs when trying to teach someone how to authentically wall/rock climb. A mixed-methods multi-phase intervention with two randomized groups – wall/rock climbing first (WF; n = 12) and MBVGs first (GF; n = 12) utilizing Xbox One’s Kinect Sports Rivals Rock Climbing – was used. All participants had no prior climbing experience. Results indicated the participants perceived MBVGs and authentic wall/rock climbing were similar concerning climbing tactics/strategies and arm movements, but were different regarding effort and leg, finger/grip, and jumping movements. Moreover, both the MBVGs and “real-life” wall/rock climbing experiences were needed for a significant difference in the participants’ perceived understanding of both the necessary motor skills and tactics/strategies needed to wall/rock climb. In sum, MBVGs may be used as a pedagogical tool to teach strategies of wall/rock climbing, particularly with beginners or those with special needs, but caution must be heeded due to perceived effort and lower extremity movement differences compared to authentic climbing.
Predicting Outcome for Early Attention Training After Acquired Brain Injury

Background The training of impaired attention after acquired brain injury is central for successful reintegration in daily living, social, and working life. Using statistical process control, we found different improvement trajectories following attention training in a group of relatively homogeneous patients early after acquired brain injury (ABI). Objective To examine the contribution of pre-injury factors and clinical characteristics to differences in outcome after early attention training. Materials and Methods Data collected in a clinical trial comparing systematic attention training (APT) with activity-based attention training (ABAT) early after brain injury were reanalyzed. Results Stroke patients (p = 0.004) with unifocal (p = 0.002) and right hemisphere lesions (p = 0.045), and those with higher mental flexibility (TMT 4) (p = 0.048) benefitted most from APT training. Cognitive reserve (p = 0.030) was associated with CHANGE and APT as the sole pre-injury factor. For TBI patients, there was no statistical difference between the two treatments. Conclusion Our study identifies indiscernible factors predicting improvement after early attention training. APT is beneficial for patients with right-hemispheric stroke in an early recovery phase. Knowledge of prognostic factors, including the level of attention deficit, diagnosis, and injury characteristics, is vital to maximizing the efficiency of resource allocation and the effectiveness of rehabilitative interventions to enhance outcomes following stroke and TBI.
A Usage-Based Proposal for Argument Structure of Directional Verbs in American Sign Language

Verb agreement in signed languages has received substantial attention for a long time. Despite the numerous analyses about the linguistic status of verb agreement, there is little discussion about the argument structure associated with “directional verbs,” also known as agreeing/agreement or indicating verbs. This paper proposes a usage-based approach for analyzing argument structure constructions of directional verbs in American Sign Language (ASL). The proposal offers low-level constructions for reported speech, non-dedicated passive and reflexive, and stance verb constructions, which capture the patterns, abstracted from recurring usage events, that are part of users’ linguistic knowledge. The approach has potential to push the field of sign linguistics in new directions of understanding the interplay of language use and structure.
Neural Processing of Naturalistic Echolocation Signals in Bats

Echolocation behavior, a navigation strategy based on acoustic signals, allows scientists to explore neural processing of behaviorally relevant stimuli. For the purpose of orientation, bats broadcast echolocation calls and extract spatial information from the echoes. Because bats control call emission and thus the availability of spatial information, the behavioral relevance of these signals is undiscussable. While most neurophysiological studies, conducted in the past, used synthesized acoustic stimuli that mimic portions of the echolocation signals, recent progress has been made to understand how naturalistic echolocation signals are encoded in the bat brain. Here, we review how does stimulus history affect neural processing, how spatial information from multiple objects and how echolocation signals embedded in a naturalistic, noisy environment are processed in the bat brain. We end our review by discussing the huge potential that state-of-the-art recording techniques provide to gain a more complete picture on the neuroethology of echolocation behavior.
A Random Forest-Based Genome-Wide Scan Reveals Fertility-Related Candidate Genes and Potential Inter-Chromosomal Epistatic Regions Associated With Age at First Calving in Nellore Cattle

This study aimed to perform a genome-wide association analysis (GWAS) using the Random Forest (RF) approach for scanning candidate genes for age at first calving (AFC) in Nellore cattle. Additionally, potential epistatic effects were investigated using linear mixed models with pairwise interactions between all markers with high importance scores within the tree ensemble non-linear structure. Data from Nellore cattle were used, including records of animals born between 1984 and 2015 and raised in commercial herds located in different regions of Brazil. The estimated breeding values (EBV) were computed and used as the response variable in the genomic analyses. After quality control, the remaining number of animals and SNPs considered were 3,174 and 360,130, respectively. Five independent RF analyses were carried out, considering different initialization seeds. The importance score of each SNP was averaged across the independent RF analyses to rank the markers according to their predictive relevance. A total of 117 SNPs associated with AFC were identified, which spanned 10 autosomes (2, 3, 5, 10, 11, 17, 18, 21, 24, and 25). In total, 23 non-overlapping genomic regions embedded 262 candidate genes for AFC. Enrichment analysis and previous evidence in the literature revealed that many candidate genes annotated close to the lead SNPs have key roles in fertility, including embryo pre-implantation and development, embryonic viability, male germinal cell maturation, and pheromone recognition. Furthermore, some genomic regions previously associated with fertility and growth traits in Nellore cattle were also detected in the present study, reinforcing the effectiveness of RF for pre-screening candidate regions associated with complex traits. Complementary analyses revealed that many SNPs top-ranked in the RF-based GWAS did not present a strong marginal linear effect but are potentially involved in epistatic hotspots between genomic regions in different autosomes, remarkably in the BTAs 3, 5, 11, and 21. The reported results are expected to enhance the understanding of genetic mechanisms involved in the biological regulation of AFC in this cattle breed.
A Longitudinal Magnetoencephalographic Study of the Effects of Deep Brain Stimulation on Neuronal Dynamics in Severe Anorexia Nervosa

Anorexia Nervosa (AN) is a debilitating psychiatric disorder characterized by the relentless pursuit of thinness, leading to severe emaciation. Magnetoencephalography (MEG)was used to record the neuronal response in seven patients with treatment-resistant AN while completing a disorder-relevant food wanting task. The patients underwent a 15-month protocol, where MEG scans were conducted pre-operatively, post-operatively prior to deep brain stimulation (DBS) switch on, twice during a blind on/off month and at protocol end. Electrodes were implanted bilaterally into the nucleus accumbens with stimulation at the anterior limb of the internal capsule using rechargeable implantable pulse generators. Three patients met criteria as responders at 12 months of stimulation, showing reductions of eating disorder psychopathology of over 35%. An increase in alpha power, as well as evoked power at latencies typically associated with visual processing, working memory, and contextual integration was observed in ON compared to OFF sessions across all seven patients. Moreover, an increase in evoked power at P600-like latencies as well as an increase in γ-band phase-locking over anterior-to-posterior regions were observed for high- compared to low-calorie food image only in ON sessions. These findings indicate that DBS modulates neuronal process in regions far outside the stimulation target site and at latencies possibly reflecting task specific processing, thereby providing further evidence that deep brain stimulation can play a role in the treatment of otherwise intractable psychiatric disorders.
Emergence of a Carbapenem-Resistant Klebsiella pneumoniae Isolate Co-harbouring Dual blaNDM– 6-Carrying Plasmids in China

The widespread emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) with limited therapeutic options has become a global concern. In this study, a K. pneumoniae strain called KP2e was recovered from a human case of fatal septic shock in a Chinese hospital. Polymerase chain reaction and sequencing, antimicrobial susceptibility testing, conjugation experiments, S1 nuclease-pulsed field gel electrophoresis/southern blot, whole genome sequencing and comparative genomics were performed to investigate the phenotypic and molecular characteristics of this isolate. KP2e possessed the NDM-6-encoding gene and exhibited resistance to almost all β-lactams except for monobactam. This strain belonged to sequence type 4024, the complete genome of which was composed of one chromosome and three plasmids. Furthermore, blaNDM–6 coexisted on two self-transmissible plasmids, which were assigned to types IncFIB and IncN. A structure of IS26-composite transposon capturing an identical Tn125 remnant (ΔISAba125-blaNDM–6-bleMBL-trpF-dsbC-cutA-groES-ΔgroEL) was identified in the two plasmids, and this conserved blaNDM-surrounding genetic context was similar to that of few IncN plasmids found in other regions of China. Our research appears to be the first description of a clinical strain that emerged co-harbouring dual blaNDM-carrying plasmids, and the first report of NDM-6-positive CRKP in China. These findings demonstrated that IncN is a key medium in the evolution and expanding dissemination of blaNDM genes among various species, which indicates that close monitoring and rapid detection of blaNDM-harbouring plasmids is necessary.
Comparative Analysis of Pseudo-nitzschia Chloroplast Genomes Revealed Extensive Inverted Region Variation and Pseudo-nitzschia Speciation

Pseudo-nitzschia is a species-rich genus where many species can induce harmful algae blooms (HABs) associated with the toxin domoic acid (DA) production. Despite the importance of Pseudo-nitzschia species to coastal environments, their genomic information is rather limited, hindering research on biodiversity and evolutionary analysis. In this study, we report full-length chloroplast genomes (cpDNAs) of nine Pseudo‐nitzschia, among which cpDNAs of eight Pseudo-nitzschia species were reported for the first time. The sizes of these Pseudo-nitzschia cpDNAs, which showed typical quadripartite structures, varied substantially, ranging from 116,546 bp to 158,840 bp in size. Comparative analysis revealed the loss of photosynthesis-related gene psaE in cpDNAs of all Pseudo-nitzschia species except that of P. americana, and the selective loss of rpl36 in P. hainanensis. Phylogenetic analysis showed that all Pseudo-nitzschia strains were grouped into two clades, with clade 1 containing cpDNAs of P. multiseries, P. pungens, P. multistriata, and P. americana, and clade 2 containing cpDNAs of P. hainanensis, P. cuspidata, Pseudo-nitzschia sp. CNS00097, P. delicatissima, and P. micropora. The small size of the P. americana cpDNA was primarily due to its shortened inverted repeat (IR) regions. While psaA and psaB were found in the IR regions of cpDNAs of other eight species, these two genes were found outside of the IR regions of P. americana cpDNA. In contrast, P. hainanensis had the largest size because of expansion of IR regions with each IR region containing 15 protein-coding genes (PCGs). Eleven genetic regions of these Pseudo-nitzschia cpDNAs exhibited high nucleotide diversity (Pi) values, suggesting that these regions may be used as molecular markers for distinguishing different Pseudo-nitzschia species with high resolution and high specificity. Phylogenetic analysis of the divergence of nine Pseudo-nitzschia species indicated that these species appeared at approximately 41 Mya. This study provides critical cpDNA resources for future research on the biodiversity and speciation of Pseudo-nitzschia species.
Non-linear Black-Scholes Option Pricing Model based on Quantum Dynamics

This paper is concerned with the option pricing based on an extended non-linear Black-Scholes model. In literature, non-linear Black-Scholes models are formulated assuming the stochastic asset price volatility, volatile risk-free interest rate or the occurrence of the transaction costs. Since these assumptions are matching better the real market conditions, these models are regarded to be more accurate in option pricing than linear BlackScholes model. In this paper the option pricing model is derived from non-linear Schrödinger equation. This equation governs the movement of quantum particles which is similar to the volatility of stock prices. The nonlinear Schrödinger equation with external potential terms is formulated. The non-linear Black-Scholes option pricing model is formulated using the transformation of the non-linear Schrödinger equation from complex Hilbert to real Euclidean space. The developed model has been used to predict European call options price based on WIG20 stock prices. The model parameters have been estimated based on real market data. The method of lines has been used to solve numerically the non-linear option pricing model. The model parameters have been estimated based on real market data. Numerical results are provided and discussed. The obtained results confirm high accuracy of the proposed non-linear model.
Assessment of Sectoral Virtual Water Flows and Future Water Requirement in Agriculture Under SSP-RCP Scenarios: Reflections for Water Resources Management in Zhangye City

Water scarcity is a core issue that constraints the high-quality development of arid areas in northwestern China. Zhangye is an oasis city located in the Heihe River Basin in northwestern China. It is populated with an agriculture-dominated economy and faces more and more serious water crises. Virtual water is an indicator that can measure the embodied water in the traded products, which has been widely applied for making rational policies for water resources management. In addition, clarifying water requirements in agricultural sectors under future climate change scenarios is essential to develop more appropriate adaptation strategies. From this perspective, this study aims to evaluate and compare virtual water flows among various sectors in Zhangye for the years 2012 and 2017 with a single regional input-output model and to further clarify the future water requirement tendency in agriculture during 2020–2050 under different shared socioeconomic pathways and representative concentration pathways (SSP-RCP) scenarios. The results showed that the planting sector directly contributed most of the total water consumption with the highest direct coefficient of 3307.5 m3/yuan in 2012, whereas the manufacture of food products and tobacco processing sector had the largest proportion of indirect water consumption (99%) mainly from intermediate inputs of agricultural products. Water consumption intensity of all sectors on average decreased by 22% during 2012–2017, indicating an increasing water utilization efficiency in economic industries. Household consumption also can improve water utilization efficiency as the major pathway for final consumption (86.4% in 2017). Water scarcity in Zhangye was becoming increasingly prominent since virtual water net exports were higher than local consumption, especially in the agriculture, manufacturing, and energy supply industries. Moreover, under climate change scenarios, we found the highest level of water requirement per unit area occurred in 2000, but it still had an incremental potential by 2050, especially in SSP585. The high requirement intensity and large-scale maize planting caused a rising tendency of total crop water requirement with an annual increasing rate of 8.4% from 1980 to 2050. This makes it possible to adapt to climate change through scientific management measures and technical means. We further made policy implications for adaptive management of water resources in Zhangye.
Synchronizing Nitrogen Fertilization and Planting Date to Improve Resource Use Efficiency, Productivity, and Profitability of Upland Rice

Synchronizing nitrogen (N) fertilization with planting date (PD) could enhance resource use efficiency and profitability of upland rice (Oryza sativa L.) production in Thailand. The objective of the study was to assess upland rice responses to four N fertilization rates (NFRs) and three planting dates. Field experiments were conducted during two growing seasons under four NFRs, no N applied (N0), 30 (N30), 60 (N60), and 90 kg N ha−1 (N90), and NFR were applied at the initiation of tillering and panicle emergence stages. The planting dates selected were early (PD1), intermedium (PD2), and late planting (PD3) between September and December of each season. The NFRs and planting dates had a significant influence on N uptake, N use efficiency (NUE), crop water productivity, yield and yield attributes, and profitability of upland rice production. A linear relationship among NFRs, agronomic traits of upland rice, N uptake, and crop water productivity was observed, and a significant seasonal effect was indicated. Fertilization at N90 under PD2 enhanced yields, yield attributes, and grain yields, as well as crop water productivity by 56 and 105% during the second and first seasons, respectively. Grain N, total N, and straw N were increased by 159, 159, and 160%, and by 90, 114, and 153%, during the first and second seasons, respectively. Enhanced N efficiencies, including agronomic efficiency, recovery efficiency, partial factor productivity, and N harvest index, at varying NFRs were observed under PD2 during both seasons. Highly significant (p < 0.001) and positive associations were observed among agronomic attributes, N uptake, NUE, and crop water productivity of upland rice in correlation assessment. Profitability from grain yields was observed with N fertilization and N90 resulted in maximum profit under all the PDs. However, the highest marginal benefit-cost ratio was observed at N60 under PD2 during both seasons. The results suggest that the NFR of 90 kg N ha−1 and planting at the end of September or start of October would enhance resource use efficiency and productivity, and maximize profitability. Furthermore, long–term field investigations with a range of NFRs and adopting forecasting measures to adjust the planting date for upland rice are recommended.
Germination Characteristics Associated With Glutathione S-Transferases Endowed Quizalofop-p-Ethyl Resistance in Polypogon fugax

Quantification of germination characteristics between herbicide-resistant and -susceptible weeds might provide methods to control resistant weeds and permit better prediction of evolution and persistence of herbicide resistance. This study aimed to compare the germination characteristics of Asian minor bluegrass (Polypogon fugax) populations that are resistant or susceptible to quizalofop-p-ethyl under controlled conditions, which the resistance mechanism is involved in glutathione S-transferases (GSTs) metabolism-based resistance. No major differences in seed germination were found at diverse temperatures, pH ranges, and light conditions. However, a significant difference that seed response to a gradient of osmotic and salt stress between the resistant and susceptible P. fugax populations were found. Two stress response genes (P5CS-1 and CDPK-2) in P. fugax were likely involved in germination rate as well as germination speed in response to these stresses. Subsequently, population verification demonstrated that P5CS-1 and CDPK-2 genes may be linked to the resistance mechanism. Additionally, the two genes play an important role in response to salt stress and osmotic stress as shown by transcript abundance after stress treatments. Our findings suggest that the variation of the germination characteristics in P. fugax associates with the presence of GST-endowed resistance mechanism.
Noncanonical PD-1/PD-L1 Axis in Relation to the Efficacy of Anti-PD Therapy

With programmed death 1/ligand 1 (PD-1/PD-L1) as the cornerstone, anti-PD antibodies have pioneered revolutionary immunotherapies for malignancies. But most patients struggled to respond to anti-PD owing to primary or acquired resistance or even hyperprogression, pointing to more efforts needed to explore this axis. PD-1 constrains T-cell immunoreactivity via engaging with PD-L1 of tumor/myeloid cells is the canonical PD-1/PD-L1 axis function mode. Studies are increasingly aware of the impact of noncanonical PD-1/PD-L1 expression in various cancers. PD-L1 induced on activated T-cells ligates to PD-1 to mediate self-tolerance or acts on intratumoral myeloid cells and other T-cells, affecting their survival, differentiation and immunophenotyping, leading to tumor immunosuppression. Myeloid PD-1 interferes with their proliferation, differentiation, cytokine secretion and phagocytosis, mediating remarkable pro-tumor effects. Tumor cell intrinsic PD-1 signaling has diverse functions in different tumors, resulting in pro-proliferation or proliferation inhibition. These nonclassical PD-1/PD-L1 functions may be novel anti-PD mechanisms or causes of treatment resistance. This review highlights the nonnegligible role of T-cell-intrinsic PD-L1 and tumor/myeloid PD-1 in the cell interplay network and the complex impact on the efficacy of anti-PD antibodies. Reconsidering and rational utilization of the comprehensive PD-1/PD-L1 axis could cumulate breakthroughs in precision treatment and combination for anti-PD therapies.