Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcription factors induced by interferons ao and -y

Factors induced by interferons (IFNs) bind to the IFN-stimulated response elements (ISREs) of many genes. In human cells treated with type I ( a, 3) IFN, factor E is induced in about 1 min and factor M after about 1 hr. Factor G is induced after about 1 hr in cells treated with type 11 (-y) IFN. G and M have very similar positions in bandshift assays, sensitivities to cycloheximide, footprints on an ISRE and relative affinities for different ISREs. Four different patterns of expression were observed in different cell lines: E,M and G strongly induced; M and G strongly but E weakly; only E and M induced; only E induced. Transcription in response to IFN a is initiated by E and probably maintained by M since, in fibroblasts, M is present maximally when transcription is most active and declines together with transcription. In Bristol-8 cells, where induction of M is not detected, transcription is still induced by IFN a and still declines in its continued presence, suggesting that M is not essential for either process. A variant ISRE with two G-to-C mutations binds E especially weakly but M and G strongly. The mutations don't change the response of a reporter gene to IFN y but do abolish its response to IFN a, suggesting that the binding of M is not sufficient for the latter. G probably acts positively, since its appearance correlates well with induction of transcription by IFN e. A 39-bp ISRE from the 9-27 gene binds E much better than M or G. Conversely, a 39-bp ISRE from the 6 - 16 gene binds M and G much better than E. Different patterns of expression of E, M, and G and different affinities of
Analysis of the EDoS attack impact on elastic cloud services using finite queuing model

This paper proposes a logical model to examine the effect of the EDoS attack in cloud environment using finite queuing model and enhanced with experimental model. Due to this sophisticated attacks the computing resources are busy and buffer capacity of the cloud gets exhausted by both the legitimate and malicious user requests, because of this both types of requests could not get the service. The legitimate customers are unable to get service of web application. In this backdrop this paper investigates and evaluates the vendor loss factor from the cost factor of view since the legitimate client requests are denied service. The objective of this analysis is twofold i) to identify the dynamics of the EDoS attacks with different attack rates and to measure the various performance metrics (total number of busy virtual machines, utilization of the cloud resources, request response time, request loss probability, and throughput). ii) The cost function is defined and evaluated based on these performance metrics. Finally compared analytical and experimental results are presented and conclusions are drawn.
Electromagnetic and thermal analysis and design of a novel‐structured surface‐mounted permanent magnet motor with high‐power‐density

A segmented-core (SC) structure has been widely used for high-power-density (HP) motors. However, the SC motor is associated with a number of problems due to the complexity of both the structure and the manufacturing process. To address these issues, a novel structure of a HP motor is proposed, referred to as the ring-coupled segmented-stator (RSS) model here. The proposed RSS can increase the reliability, the stability, and the manufacturability of motor. Furthermore, useful thermal analysis and design flow which take into account the RSS and asymmetric overhang structure of the motor are proposed in this research. The proposed lumped parameter thermal network (LPTN) for the thermal analysis shows a good agreement with experimental data within 9.8% difference. The proposed analysis and design method can be used for the diverse kinds of motor requiring the HP. The usefulness of the proposed RSS motor, the analysis method, and the design method are verified through the experiment in this research.
Suppression of uPA and uPAR Blocks Radiation-induced MCP-1 mediated Recruitment of Endothelial Cells and Monocytes in meningioma

Chemokines play a vital role in recruiting various cell types in the process of tissue repair. Radiation, a major therapeutic modality in cancer treatment, has been described to induce inflammatory response that might lead to the expression of several chemokines. In the present study, we investigated the mechanism of monocyte chemoattractant protein-1 (MCP-1) induction by radiation in meningioma cell lines and the paracrine effect on human microvascular endothelial cells (HMEC). After radiation, meningioma cell lines (IOMM Lee and SF-3061) showed an increased expression of MCP-1. In addition, irradiated meningioma cancer cell conditioned medium (CM) showed increased ability to attract HMEC and stimulate MCP-1-induced protein (MCPIP), VEGF and angiogenin expression in HMEC. This chemotactic activity and angiogenic stimulator effect on HMEC was almost abrogated by depleting MCP-1 from the irradiated cancer cell CM. Further, inhibition of either ERK activation/expression or NF- κ B nuclear translocation hindered radiation-induced MCP-1 expression in both meningioma cell lines. Further, supplementing cancer cells with exogenous ATF-uPA (with and without radiation) activated ERK phosphorylation, nuclear translocation of the NF- κ B p65 sub-unit (Rel-A), and MCP-1 expression. Downregulation of uPA and uPAR, simultaneously by transfecting the cancer cells with bi-cistronic siRNA-expressing plasmid (pU) inhibited radiation-induced ERK activation, nuclear translocation of Rel-A, NF- κ B DNA binding activity, and MCP-1 expression. In addition, pU-transfected cancer cells (with or without radiation) reduced radiation-induced MCP-1 and blocked the recruitment of other cell types during the inflammatory process induced by radiation both in in vitro and in vivo conditions. ImageJ software, and are represented graphically. Results from three independent experiments were evaluated and presented (mean S.D value; p <0.05).
Relationship between Length of Gestation, Birth Weight and Certain Other Factors* A Statistical Study

It is well known that there is a relationship between the weight of the foetus and the length of gestation, but both these elements may be affected by a variety offactors which may be of importance to the viability and developmental potential of premature infants and of those born at term. A number of these factors are considered in this study, which is based on data in the literature and on relatively extensive case materialfrom Poland. Statistical analysis suggests that there is slight correlation between the weight of the foetus on the one hand and, on the other, the weight of the placenta, the height of the mother and the age of the mother. The author concludes, inter alia, that the relation between foetal weight and length of gestation, being easily determined, is of value in routine clinical practice as a general guide to the degree of maturity of an infant, but that other more precise measures need to be developed.
3-Adrenoceptor agonist stimulation of acid secretion by rat stomach in vitro is mediated by 'atypical' ,B-adrenoceptors

1 A previous study showed P-adrenoceptor agonists stimulated acid secretion by rat stomach in vitro. The receptors could not be classed as either the ,B,- or P2-subtype. This study examines the effect of 2 'atypical' P-agonists on acid secretion. 2 Basal and isoprenaline-stimulated acid secretion were compared in tissues bathed either in HEPES/ 02- or HCOT/CO2-buffer. Basal secretion was underestimated in HCOT by an amount equal to the rate of base section. Tissues responded well in HEPES buffer and there was no base secretion following acid inhibition with SCH 28080. HEPES was used for the study. 3 SR 5861 1A stimulated acid in a concentration-related way (0.1-5 ! M). Maximum response at 1 JM was equal to the response to a maximal concentration of isoprenaline. BRL 37344 (1 LM) also stimulated to the same extent. 4 Responses to isoprenaline (5 gM) and SR 5861 1A (1 ! LM) were reduced by propranolol (1O rM) but not by alprenolol (10 tM) or by practolol (12.5IM) plus ICI 118551 (1 tM). 5 Exposure to SR 5861 1A (1 AM) led to desensitization to isoprenaline but not to bethanechol (1 1M) or histamine (50 gM). 6 We conclude that a HEPES/02-buffer is advantageous when measuring gastric acid secretion in vitro and the stimulatory effect of P-adrenoceptor agonists is mediated by 'atypical' receptors.
Exploring tandem wing UAS designs for operation in turbulent urban environments

The stability of small unmanned air systems can be challenged by turbulence during low-altitude flight in cluttered urban environments. This paper explores the benefits of a tandem wing aircraft configuration with the implementation of a pressure-based phase-advanced turbulence sensory system on a small unmanned air system for gust mitigation. The objective was to utilise passive and active methods to minimise gust-induced perturbations. Experimentation in repeatable turbulence within a wind tunnel’s test section was conducted. The experiments focus on the roll axis, which is isolated through a specially designed roll-axis rig. The results show improvement over conventional aircraft. This work is part of a larger research project aimed at enabling safe, stable and steady small unmanned air systems flight in urban environments.
Childhood Obesity – 2010: Progress and Challenges

Summary The worldwide prevalence of childhood obesity has increased greatly over the past 3 decades. The increasing occurrence in children of disorders, such as type 2 diabetes, is believed to be a consequence of this obesity epidemic. Much progress has been made in understanding the genetics and physiology of appetite control and from this, the elucidation of the causes of some rare obesity syndromes. However, these rare disorders have so far taught us only limited lessons on how to prevent or reverse obesity in most children. Calorie intake and activity recommendations need to be re-assessed and better quantified, on a population level, given the more sedentary life of children today. For individual treatment, the currently recommended calorie prescriptions may be too conservative given the evolving insight on the “energy gap. ” Whilst quality of research in both prevention and treatment has improved, there is still a need for high-quality multi-centre trials with long-term follow-up. Meanwhile, prevention and treatment approaches that aim to increase energy expenditure and decrease intake need to continue. Most recently, the spiralling increase in obesity prevalence may be abating for children. Thus, even greater efforts need to be made on all fronts to continue this potentially exciting trend. are those of the authors and are not to be construed as reflecting the views of the U.S. Public Health Service. Search strategy: We identified original research, reviews, and commentaries by searching computer databases—e.g., Medline, PsychINFO, Agricola, Lexis-Nexis—and by reviewing issues of journals that publish obesity research. We directed special attention towards publications since 2002. Research developments and published work were also identified by discussions with specialists in the areas of paediatric obesity, nutrition, and public health. evidence the of this manuscript.
Performance Improvement of Semicontrolled Open-Winding PMSGs Based on Virtual Zero-Crossing Detection and Segment Injection of Third-Order Harmonic Current

The semicontrolled open-winding permanent magnet synchronous generators (OW-PMSGs) with a common dc bus system have received more attention in recent years due to its advantages of reduced cost and simpler power supply. Good control performances could be achieved under the zero power factor angle (PFA). However, current clamping problem is normally presented under non-zero PFA condition, resulting in unexpected torque pulsations and voltage pulse disturbances. Meanwhile, misjudgment of voltage vectors and subsequent deteriorations in steady-state performances can be observed at current zero-crossing points. In order to improve the control performances of semicontrolled OW-PMSGs, a virtual zero-crossing detection method is proposed in this paper to effectively suppress the oscillation of voltage vectors at current zero-crossing points. In addition, a segment injection strategy of third-order harmonic current is proposed. With the proposed strategy adopted, the current clamping problem can be successfully solved, and more importantly, the torque ripple and third-order harmonic current could be simultaneously suppressed. Relevant experimental validations and performance comparisons based on a 2.3-kW semicontrolled OW-PMSG system are presented to verify the proposed control strategies.
TRAINING PROSOCIAL BEHAVIORS TO YOUNG CHILDREN: AN ANALYSIS OF RECIPROCITY WITH UNTRAINED PEERS

We examined the effects of a social skills training package on the play behaviors of three young girls. Two children were taught to invite their peers to play and to use social amenities during their conversations with other children. A combined reversal and multiple baseline across responses design demonstrated that both children directed more social behaviors to their classroom peers after training and that these two children's play invitations were maintained in the later absence of experimental contingencies. In addition, both target children received a greater number of play invitations from their peers during the free play periods. In contrast, a third child's play invitations were not reciprocated by peers; her invitations subsequently decreased in rate after training was discontinued. An interdependent group contingency produced a reciprocal exchange of invitations between this child and her classroom peers. A reversal design demonstrated partial maintenance of subject-peer exchanges after the group intervention was discontinued. The results obtained with the three target children suggest that peer reciprocity may facilitate the maintenance of children's play invitations over time.
CETP Genotype Modifies the Effect of Apolipoprotein ε 4 on Memory Decline in Older Adults

Apolipoprotein ε 4 (ApoE4) is a strong genetic risk factor for sporadic Alzheimer’s disease (AD) and memory decline in older adults. A single-nucleotide polymorphism in the cholesteryl ester transfer protein (CETP) gene (isoleucine to valine; V405) is associated with slower memory decline and a lower risk of AD. As both genes regulate cholesterol, we hypothesized that the favorable CETPV405 allele may buffer the effect of ApoE4 on memory decline in older adults. Using linear regression, we examined the interactive effect of ApoE4 by CETPV405 on memory decline among 909 community-dwelling, nondemented, older adults (≥70 years) from the Einstein Aging Study. Episodic memory was measured using the picture version of the Free and Cued Selective Reminding Test with immediate recall (pFCSRT+ir). There was a significant ApoE × CETP interaction on decline in pFCSRT+ir scores (p=0.01). ApoE4 carriers experienced faster decline than non-carriers among CETPI405I homozygotes (p=0.007) and in CETPI405V heterozygotes (p=0.015), but not in CETPV405V homozygotes (p=0.614). Results suggest that the CETPV405 allele buffers ApoE4-associated memory decline in a gene dose-dependent manner.
Effectiveness of Good Manufacturing Practice Training for Food Manipulators

Introduction: Good hygienic practices in food preparation should be rigorously applied in food and nutrition units (UANs), which are essential to ensure safe food, and when not properly performed, can make food into agent vehicles of foodborne diseases (DTAs). Foodborne illnesses known to be DTAs are one of the major public health problems. In this way the inability of individuals to handle food has been indicated as one of the main causes of outbreaks of food-borne diseases that are directly related to food contamination. The causes range from the lack of information on personal hygiene, ambient hygiene, improper practices in the operation of the system of production of meals, conservation or distribution in improper conditions. Materials and methods: This is a cross-sectional descriptive study. Held in the period between March and May 2018, in an institution of higher education. (IES), in coffee shops and restaurants located in, Salvador - BA. The study consisted of three stages. The first step was the application of a questionnaire with questions and answers to evaluate the previous knowledge about good manufacturing practices and raise questions for training, the second stage consisted of a previous evaluation and then the training, and the third was constituted in the questionnaire to compare the effectiveness of the training. Conclusion: The results showed that among the subjects that presented the best standard of responses after the training were considered personal hygiene, ambient hygiene, equipment and utensils and temperature control, possibly topics of greater knowledge, since in courses and orientations these are the mainly addressed.
INHIBITION OF RESPIRATORY SYNCYTIAL VIRUS INFECTION IN CELL CULTURES AND IN MICE WITH MORPHOLINO OLIGOMERS

Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infection in infants, young children, and high-risk adults. Currently, there is no vaccine for the prevention of RSV infection, and available therapeutics are of limited utility. Peptide conjugated phosphorodiamidate morpholino oligomers (PPMO) are a class of antisense agents that can enter cells readily and interfere with viral protein expression through steric blocking of complementary RNA. Two antisense PPMO, designed to target sequence that includes the 5 ′ terminal- and translation start site-regions of RSV L mRNA, were tested for anti-RSV activity in cultures of two human airway cell lines. Both PPMO showed minimal cytotoxicity, and one of them (AUG-2), reduced viral titers by more than 2.0 log 10 . Intranasal treatment of BALB/c mice with AUG-2 PPMO prior to RSV inoculation produced a reduction in viral titer of 1.2 log 10 in lung tissue at day 5 postinfection, and attenuated pulmonary inflammation at day 7 post-infection. These data show that the AUG-2 PPMO possessed potent anti-RSV activity and is worthy of further investigation as a candidate for therapeutic development.
EPIDEMIOLOGICAL SCREENING OF LYMPHATIC FILARIASIS AMONG IMMIGRANTS USING DIPSTICK COLLOIDAL DYE IMMUNOASSAY

We have recently reported that a dipstick colloidal dye immunoassay (DIA) that detect parasite antigens in human serum is sensitive and specific for the diagnosis of active infection of lymphatic filariasis. Rabbit polyclonal antibodies (RbBmCAg) labelled with a commercial dye, palanil navy blue was used to detect filarial antigenemia among Indonesian and Bangladeshi immigrant workers (N= 630) at oil palm estates at Hulu Trengganu District, Peninsular Malaysia. Microfilaremia with Brugia malayi were detected in 51 (8.10 %) individuals, of which 42 (6.67 %) were among the Indonesians and 9 (1.98 %) among the Bangladeshis. Microfilaremia with Wuchereria bancrofti were detected in 33 (5.24 %) individuals of which 15 (2.38 %) were among the Indonesians and 18 (2.86 %) among the Bangladeshis workers. The DIA detected 96 (15.24 %) antigenemic cases which comprise of all the microfilaremic cases and 15 (2.38 %) amicrofilaremic cases. The amicrofilaremic cases with filarial antigenemia consisted of 9 (1. 43 %) Indonesians and 6 (0.95%) Bangladeshis. We have used 6 ul of the RbBmCAg and diluted (1:10) patients’ sera per dipstick which make the DIA reagent conservative. The DIA is a rapid test and can be read in approximate 2 hours. Additionally, coloured dots developed in the DIA can be qualitatively assessed visually for intensity. The DIA does not require sophisticated equipment or radioactivity, and therefore suitable for field application. sonicated. The extract was obtained by centrifuging at 20 000 x g at 4 o C for 20 min and the protein content was determined by u.v absorbence at 280 and 290 nm and adjusted to 15 mg/ml. The polyclonal antibodies against B. malayi circulating antigens were raised in rabbits (RbBmCAg) by immunizing with a mixture of 0.75 ml of the antigen in 0.75 ml Freund’s complete adjuvant subcutaneously at weekly interval for three such doses. The rabbits were bled for sera 10 days after the last dose and sera were seperated by centrifugation and stored at –20 o C. Our previous studies indicated that the polyclonal RbBmCAg bind specifically to circulating antigens of B . malayi and W. bancrofti but not to circulating antigens of
Color-Independent Visible Light Communications Based on Color Space: State of the Art and Potentials

Color independency is an important factor in visible light communication (VLC) systems. This paper aims to review and summarize recent achievements in color-independent visible light communication based on color space, with the main focus being on color-space-based modulation (CSBM), termed as generalized color modulation (GCM), which allows VLC to adapt to any target color. The main advantages of GCM are its color independency, reasonable bit error rate (BER) performance during color variation, and dimming control. We also address our past research works that aimed to achieve a color-independent visual MIMO system by incorporating the advantages of GCM, which can lead to higher data rates over longer distances and improved performance, using image processing in addition to color independency. Finally, two case studies are introduced to demonstrate the potential applicability of a color-independent visual-MIMO system using color-space-based modulation techniques.
An autoinhibitory role for the Pleckstrin Homology domain of ITK and its interplay with canonical phospholipid recognition

Pleckstrin homology (PH) domains are well known as phospholipid binding modules yet evidence is mounting that PH domain function extends beyond lipid recognition. In the current work, we characterize a protein binding function for the PH domain of Interleukin-2-inducible tyrosine kinase (ITK), an immune cell specific signaling protein that belongs to the TEC family of non-receptor tyrosine kinases. Its N-terminal Pleckstrin homology (PH) domain is a well characterized lipid-binding module that localizes ITK to the membrane via phosphatidylinositol (3,4,5)-trisphosphate (PIP 3 ) binding. Using a combination of NMR spectroscopy and mutagenesis, we have mapped an autoregulatory protein interaction site on the ITK PH domain that makes direct contact with the catalytic kinase domain of ITK inhibiting the phospho-transfer reaction. Moreover, we have elucidated an important interplay between lipid binding by the ITK PH domain and the stability of the autoinhibitory complex formed by full length ITK. The ITK activation loop in the kinase domain becomes accessible to phosphorylation to the exogenous kinase LCK upon binding of the ITK PH domain to PIP 3 . By clarifying the allosteric role of the ITK PH domain in controlling ITK function we have expanded the functional repertoire of the PH domain generally and opened the door to alternative strategies to target this specific kinase in the context of immune cell signaling. we set out to characterize the regulatory role of the ITK PH domain in detail using a range of biochemical approaches and solution NMR spectroscopy. Our findings suggest that the ITK PH domain contacts the ITK kinase domain directly and exerts an inhibitory effect on ITK catalytic activity. We identify a specific surface of the ITK PH domain that mediates contacts with the ITK kinase domain and show that mutation of this region of the N-terminal PH domain activates ITK kinase activity. The soluble head group of PIP 3 , inositol (1,3,4,5)-tetrakisphosphate (IP 4 ), competes with the regulatory interaction between ITK PH and kinase domains consistent with the long standing observation that PIP 3 production contributes to ITK activation in the activated T cell. Moreover, we find that PIP 3 containing liposomes full length but not an that lacks the PH domain. Finally, we show that phospholipid engagement of the PH domain exposes the