Advances in Clinical and medical Dermatology and Therapies Combinatorial Biologics for Skin Rejuvenation Activating the Tissue Regen- eration

Epithelial tissues like those that form the skin are essential to protect the human body from diverse aggressions. The skin is the largest organ of the human body and consists of several layers and structures. It is an essential physical barrier that protects us from the external environment (radiation, xenobiotics, microorganisms, etc.) but also intervenes in essential processes, such as thermoregulation, metabolism, the homeostasis of fluids, sensing, among others [1-2]. Given its importance, damage to the skin lead to disorders of different natures that can eventually lead to diseases. When the skin integrity is compromised by a multitude of factors including mechanical, chemical, vascular, infectious, allergic, inflammatory, intrinsic, injuries, etc; can lead to many negative effects in the homeostasis. Skin appearance is important at all levels, since some attributes [3-4]. This protocol is based on our principle of combinatorial biologics from natural sources including Lycopersicon esculentum extract on oral supplement and Bioquantine® boosting the effect on the facial cream and the BioActivaded Platelet Rich Plasma (PRP + Bioquantine®) showing the improved outcomes after a 8 weeks protocol. Our focused analysis is based on the prevention and protection given by Lycopersicon esculentum. The skin rejuvenation offered by Bioquantine® and the obtained skin regeneration through PRP facial applications. Lycopersicon esculentum is also involved in several healthpromoting actions [5]. The benefit of adding a topical product containing Bioquantine® on its formula boosts then effect of the skin rejuvenation combined. Bioquantine®, a miRNA extract from Xenopus laevis frog oocytes (purified from intra and extra oocyte liquid phases of electroporated oocytes), showed potential as a treatment for a wide range of conditions in animal models, including skin rejuvenation among others, [6-8]. Bioquantine® is a first generation, combinatorial biologic polypeptide, by molecular structure it sizes less than 0.22 microns it intends to mimic the regulatory biochemistry of the living oocyte, immediately after a fertilization event by biofactors induced by spermatozoa binding signal. These bio factors are responsible for: resetting cellular age (i.e creating stem cells), reprogramming DNA to eliminate genetic and epigenetic damage, remodeling critical energy producing organelles (such as mitochondria). And protecting of the embryo from inflammatory, oxidative and infectious factors. The current abstract observed beneficial changes with the combinatorial biologics use. Platelet-rich plasma (PRP) has gained popularity in facial procedures because of its role in tissue repairing. PRP, having a higher concentration of platelets, allows for greater release of growth factors and biologically active proteins, these can even being considered virtually every step of embryogenesis. There are on the order of several hundred genes whose products communicate from the extracellular space influencing the transmembrane intracellular receptors stimulating the signaling pathways [9]. Platelet growth factors have pivotal roles (EGF, epidermal growth factor; FGF, fibroblast growth factor; PDGF, platelet derived growth factor; TGF-β, transforming growth factor beta; VEGF, vascular endothelial growth factor) [10]. These factors cause migration of mesenchymal stem cells (MSC) and endothelial cells as a functional response to the factors released (Plateletrich Concentrates Differentially Release Growth Factors and Induce Cell Migration in Vitro). Going into the molecular mechanisms of stem cell maintenance and determination in the skin using signaling, growth factors is proven and possible successfully distinguish putative bulge stem cells from their progeny. Some of these factors have been previously associated with stimulation of different cell types that surround the bulge area, while others are likely involved in extracellular matrix and basement membrane organization. Still others are candidates for creating gradient of signaling molecules that could maintain stem cells within the bulge area in a growth and differentiation. These findings are in agreement with the bulge being an entity with tissue morphogenesis [11]. One of the most popular uses for PRP is for facial skin rejuvenation in the form of dermal injections and topical application during microneedling [12] for skin rejuvenation, regeneration, and reconstruction; improving wrinkling among other benefits [13]. By BioActivated PRP we designated the boosted 2ml of ordinary PRP by adding 0.2 ml of Bioquantine®. As part of our 60 days skin rejuvenation protocol we also added a formulated cream to support the