Draft Genome Sequence of the Plant Growth-Promoting Bacterium <named-content content-type='genus-species'>Pseudomonas pseudoalcaligenes</named-content> KB-10

Pseudomonas pseudoalcaligenes KB-10 can enhance salinity tolerance in coriander plants. We report a draft genome sequence of P. pseudoalcaligenes KB-10, comprising a 5,241,174-bp circular chromosome containing 4,921 genes, with a GC content of 62.97%. Plant growth-promoting bacteria (PGPB) can enhance plant growth and tolerance to various abiotic stresses including salinity (1). Here, we report the draft genome sequence of Pseudomonas pseudoalcaligenes strain KB-10. This bacterium was isolated from the rhizosphere of alfalfa (Medicago sativa L.) growing at the Agricultural Research Station of King Abdulaziz University located at Hada Al-Sham, Jeddah, Saudi Arabia (2). The strain was isolated using nutrient agar medium with an incubation period of 48 h at 30°C. The purified strain was stored in a 20% glycerol solution at 280°C for subsequent use. The strain was assessed for its ability to tolerate NaCl, to produce indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate deaminase, and to solubilize tricalcium phosphate. The strain was able to tolerate up to 5% NaCl and to produce indole-3-acetic acid (2). A pot experiment revealed that the isolated strain augmented plant growth and salinity tolerance in coriander (Coriandrum sativum L.) (2). For genomic DNA isolation, a single colony of the strain was grown in nutrient broth overnight at 30°C. The genomic DNA was extracted using a genomic DNA purification kit (MGmed, South Korea) according to the manufacturer's instructions. A DNA library was generated using the TruSeq DNA Nano sample preparation kit (Illumina, Inc., CA, USA), and then paired-end (2 100-bp) sequencing was performed using the HiSeq 4000 platform (Illumina, Inc.) at Macrogen (South Korea), generating a total of 1,702,568,312 bp from 16,857,112 reads. After sequencing, FastQC version 0.11. 6 (3) was used in order to check the read quality. In order to reduce biases in the analysis, an in-house script was used to filter reads if less than 90% of the bases that made up the read had a score below Q20. Trimmomatic version 0.36 (4) was used to remove the adapter sequences. After filtering, genome sequencing yielded 1,094,661,536 bases from 11,050,922 reads. For de novo assembly, a genome survey was performed with Jellyfish version 1.1. 11 (5) to first estimate the genome size of the sample. In this step, 17, 21, and 25 k-mers were used for calculation of the estimated genome size, and the k-mer plot was expressed with GenomeScope (6). After the genome survey, the initial de novo genome assembly was performed with various k-mer values with SOAPdenovo2 version 2.04 (7), and the gapclosing process was performed with GapCloser version 1.112-r6, which resulted in 28 contigs ranging from 1,135bp to 1,079,592bp. Prokka version 1.12b was used for gene prediction and annotation (8). The assembled sequences were also used for searches against the GenBank nonredundant, UniProt, GO, InterPro, Pfam, conserved domain (CD), TIGRFAM, and eggNOG databases using BLAST1 version 2.6. 0 for additional annotation (9). Default parameters were used for all software unless otherwise mentioned. Citation Khan MMA, Duan J, Glick BR, Finnegan PM, Kabli SA, Al-Garni SMS. 2021. Draft genome sequence of the plant growthpromoting bacterium Pseudomonas pseudoalcaligenes KB-10. Microbiol Resour Announc 10:e00311-21. https://doi.org/10 . 1128/MRA.00311-21. Editor David A. Baltrus, University of Arizona Copyright © 2021 Khan et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to M. M. A. Khan, mohibulalamkhan@gmail.com. Received 24 March 2021 Accepted 4 May 2021 Published 20 May 2021 Volume 10 Issue 20 e00311-21 mra.asm. org 1 GENOME SEQUENCES