Miracle-dz/UniFAR-FTUs huggingface dataset:
Visualization of yellow fever virus infection in mice using a bioluminescent reporter virus

ABSTRACT Yellow fever virus (YFV) is a re-emerging flavivirus, which can lead to severe clinical manifestations and high mortality, with no specific antiviral therapies available. The live-attenuated yellow fever vaccine 17D (YF17D) has been widely used for over eighty years. However, the emergence of yellow fever vaccine-associated viscerotropic disease (YFL-AVD) and yellow fever vaccine-associated neurotropic disease (YFL-AND) raised non-negligible concerns. Additionally, the attenuation mechanism of YF17D is still unclear. Thus, the development of convenient models is crucial to understand the mechanisms behind YF17D attenuation and its adverse effects. In this work, we generated a reporter YF17D expressing nano-luciferase (NLuc). In vitro and in vivo characterization demonstrated that the NLuc-YF17D shared similar biological properties with its parental strain and the NLuc activity can reflect viral infectivity reliably. Combined with in vivo bioluminescence imaging, a series of mice models of YF17D infection was established, which will be useful for the evaluation of antiviral medicines and novel vaccine candidates. Especially, we demonstrated that intraperitoneally (i.p.) infection of NLuc-YF17D in type I interferon receptor-deficient mice A129 resulted in outcomes resembling YEL-AVD and YEL-AND, evidenced by viral replication in multiple organs and invasion of the central neuronal system. Finally, in vitro and in vivo assays based on this reporter virus were established to evaluate the antiviral activities of validated antiviral agents. In conclusion, the bioluminescent reporter virus described herein provides a powerful platform to study YF17D attenuation and vaccine-associated diseases as well as to develop novel countermeasures against YFV.
Transmuscular quadratus lumborum block versus oblique subcostal transversus abdominis plane block for analgesia in laparoscopic hysterectomy: a randomised single-blind trial

Objective The transmuscular quadratus lumborum (TQL) block and the oblique subcostal transversus abdominis plane (OSTAP) block both contribute to multimodal analgesia after laparoscopic surgery. The objective of this study was to compare the analgesic effects of the TQL block versus OSTAP block after laparoscopic hysterectomy. Design Prospective single-centre randomised single-blind trial. Setting University-affiliated hospital. Participants Patients aged between 18 and 65 years scheduled for laparoscopic hysterectomy. Interventions Patients were randomised into two groups (1:1 ratio) and received bilateral TQL block or bilateral OSTAP block with 0.375% ropivacaine 20 mL on each side before surgery. Primary and secondary outcome measures The primary outcome measure was the cumulative morphine dose in the first 24 hours. The secondary outcome measures were the morphine consumption at each time interval after surgery, the time from the end of surgery to the first need for morphine, the Numerical Rating Scale (NRS) scores for visceral and incisional pain intensity, and the incidence of adverse events. Results The cumulative morphine dose was significantly lower in the TQL group than in the OSTAP group (17.2 (12.5) vs 26.1 (13.3) mg, p=0.010). Compared with the OSTAP group, the morphine doses from 6 to 12, 12 to 18, and 18 to 24 hours were significantly lower, the time of first need for morphine was significantly longer and the NRS scores for visceral pain intensity were significantly lower in the TQL group. Conclusion Compared with the OSTAP block, the TQL block reduced morphine consumption and provided better visceral pain relief with a longer duration of effect after laparoscopic hysterectomy. Trial registration number Chinese Clinical Trial Registry (ChiCTR1800017995); pre-results.
Effects of glucocorticoids on the levels of serum tumor necrosis factor alpha and interleukin 6 in patients with rheumatoid arthritis.

OBJECTIVE The study was designed to explore the effects of glucocorticoid therapy on the levels of serum interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) in patients with rheumatoid arthritis (RA). METHODS Clinical information of 100 patients with RA who were admitted to our hospital from 2015 to 2018 were retrospectively collected and divided into two groups according to the random number table method. Patients receiving routine treatment were classified as the control group (n = 50) and those receiving glucocorticoid therapy based on routine treatment were classified as the observation group (n = 50). Pre- and post-treatment clinical effects, tender joint counts, swollen joint counts; periods of morning stiffness, visual analog scale (VAS) scores, Disease Activity Score-28 (DAS28), erythrocyte sedimentation rate (ESR), and rheumatoid factor (RF), IL-6, and TNF-α levels were compared between the two groups. RESULTS Compared with the control group, the observation group had a higher total effective rate. The observation group exhibited lower tender and swollen joint counts and shorter morning stiffness periods than the control group (P < 0.05). The VAS scores and DAS28 in the observation group were significantly lower than those in the control group (P < 0.05). The ESRs and RF levels as well as the post-treatment IL-6 and TNF-α levels were lower in the observation group than in the control group (P < 0.05). CONCLUSION Glucocorticoids show beneficial effects on alleviating RA symptoms. Due to the limited sample size in the study, future studies with a larger cohort and over a longer investigation period are warranted to provide comprehensive results.
Effect of NaOH molarities to the microstructure and sodium storage performance of the Sn-MOF derived SnO2 microporous rod

In this study, we demonstrated a facile method to prepare a novel SnO2 microporous rod with various microstructures by controlling NaOH molarities in precursor synthesis processes. Four different molarities of NaOH solution (0.005 M, 0.048 M, 0.12 M and 0.5 M) were used together with o-phthalic acid in Sn-MOF synthesis to determine the effect of ligand [o-C6H4 CO222− ] concentration on microstructure evolution. It was found that increasing NaOH molarity can effectively decrease the size of Sn-MOF rods. Then, the SnO2 microporous rods were obtained by calcinating the as-prepared Sn-MOF as microstructures. Under an optimized experimental condition (NaOH molarity of 0.12 M), the SnO2 rods shows a modest initial coulombic efficiency of 61.3% with a high reversible sodium storage capacity of 503 mAh g−1 after 150 cycles at 50 mA g−1. Moreover, an impressive reversible sodium storage capacity of 206 mAh g−1 can be obtained at long-term cycling performance (800 cycles at current density of 2 A g−1). Effects of morphologies to electrochemical performances have been further discussed in aspects of intrinsic resistance, pseudocapacitive contribution, surface area and porous structure and microstructural stability, and the enhanced electrochemical performance could be attributed to factors of enhanced pseudocapacitive charge contribution, optimized microstructures, and structural stability, which ensure the SnO2−0.12 M to have a good rate performance and cyclability. This nanoscale-engineering method adopted here could be a promising path to fabricate SnO2-based anodes with novel microstructures for sodium storage applications.
Light-Emitting Conjugated Organic Polymer As An Efficient Fluorescent Probe for Cu2+ Ions Detection and Cell Imaging.

Conjugated organic polymers (COPs) have been excellent candidates because the conjugated structure occupied π structure that was useful to develop light-emitting materials. However, most COPs emitted weak luminescence owing to the H-aggregation effect. Light-emitting conjugated organic polymers (LCOP-1) possessed rich butyl groups anchored in the skeleton to enhance light-emitting activity via reducing the H-aggregation effect. Owing to abundant hydroxyl and nitrogen atoms, LCOP-1 exhibited high sensitivity, selectivity, and fast response to Cu2+ ions within one minute in comparison with the cations of Na+ , Mg2+ , Al3+ , Zn2+ , Cd2+ , Ni2+ , Cr3+ , Hg2+ , Fe3+ , Fe2+ , Pb2+ , Co2+ , etc. The detection limit could be down to nanomolar. Moreover, the sensor exhibited detection toward Cu2+ ions via a naked eye colourful change from pale-yellow to yellowish-brown. Furthermore, the light-emitting probe also successfully achieved the detection of Cu2+ ions in cells without cytotoxicity, indicating its great potentials in biological function. This article is protected by copyright. All rights reserved.
Review of vibration‐based energy harvesting technology: Mechanism and architectural approach

Energy harvesting technologies are growing rapidly in recent years because of limitation by energy storage and wired power supply. Vibration energy is abundant in the atmosphere and has the potential to be harvested by different mechanisms, mainly through piezoelectric and electromagnetic means. Various architectural structures were also designed for several operating conditions, namely, resonance frequency and range thereof, acceleration, and energy extraction from several motions. The advantages and disadvantages were elaborated on, and improvements on ideas from current research were discussed in this review.
Thermally Induced Transformation of Nonhexagonal Carbon Rings in Graphene-like Nanoribbons

Exploring the structural transformation of nonhexagonal rings is of fundamental importance for understanding the thermal stability of nonhexagonal rings and revealing the structure–property relationships. Here, we report on the thermally induced transformation from the fused tetragon-hexagon (4–6) carbon rings to a pair of pentagon (5–5) rings in the graphene-like nanoribbons periodically embedded with tetragon and octagon (4–8–4) carbon rings. A distinct contrast among tetragon, pentagon, hexagon, and octagon carbon rings is provided by noncontact atomic force microscopy with atomic resolution. The thermally activated bond rotation with the dissociation of the shared carbon dimer between the 4–6 carbon rings is the key step for the 4–6 to 5–5 transformation. The energy barrier of the bond rotation, which results in the formation of an irregular octagon ring in the transition state, is calculated to be 1.13 eV. The 5–5 defects markedly change the electronic local density of states of the graphene-like nan.
Treatment Intensity, not Age, Affects Survival Time of Patients with Advanced Pancreatic Cancer.

BACKGROUND AND AIMS Despite older-aged individuals accounting for most patients with pancreatic cancer, elderly patients are still underrepresented in the clinical trials. Our study aims to identify treatment differences as well as to analyze survival times in the younger and older patient group. METHODS We evaluated the data of 97 pancreatic cancer patients (72 <75 years; 25 ≥75 years) receiving palliative chemotherapy. Age, comorbidity, body mass index (BMI), tumor localization, metastases, carbohydrate-antigen 19-9 (CA19-9) value, number and type of chemotherapeutic agents and treatment regimens used, treatment lines, toxicity and survival time were assessed. RESULTS The age groups did not differ in their initial conditions (comorbidity, BMI, tumor characteristics). However, treatment intensity of patients ≥ 75 years was lower. Elderly patients received significantly fewer different chemotherapeutic agents and therapeutic regimens, therapy lines and fewer combination chemotherapies. Moreover, elderly patients survived significantly shorter (7.6 vs. 12.7 months, p=0.001). In multivariance analysis, a significant negative influence on survival time was revealed for low therapy intensity (≤2 chemotherapeutics, ≤2 therapy lines), but not for age. In addition, therapy discontinuation and underweight were significantly associated with survival time. CONCLUSION Not age per se but lower therapy intensity leads to a shorter overall survival in the elderly patient group.
Childhood overweight and obesity in Europe: Changes from 2007 to 2017

The Childhood Obesity Surveillance Initiative (COSI) routinely measures height and weight of primary school children aged 6–9 years and calculates overweight and obesity prevalence within the World Health Organization (WHO) European Region using a standard methodology. This study examines the trends in the prevalence of overweight and obesity from the first round of COSI carried out in 2007/2008 to the latest of 2015/2017 in 11 European countries in which data were collected for at least three rounds. In total 303,155 children were measured. In general, the prevalence of overweight and obesity among boys and girls decreased in countries with high prevalence (Southern Europe) and remained stable or slightly increased in Northern European and Eastern European countries included in the analysis. Among boys, the highest decrease in overweight (including obesity) was observed in Portugal (from 40.5% in 2007/2008 to 28.4 in 2015/2017) and in Greece for obesity (from 30.5% in 2009/2010 to 21.7% in 2015/2017). Lithuania recorded the strongest increase in the proportion of boys with overweight (from 24.8% to 28.5%) and obesity (from 9.4% to 12.2%). The trends were similar for boys and girls in most countries. Several countries in Europe have successfully implemented policies and interventions to counteract the increase of overweight and obesity, but there is still much to be done.
Fit for the future: embracing a name change of IFOMPT to International Federation of Musculoskeletal Physical Therapists

The vision of the International Federation of Orthopedic Manipulative Physical Therapists (IFOMPT) presented in its Strategic Plan (2020– 2025) [1], is to take the organization into the future as a relevant and leading international body both within the profession and the wider community. Toward actioning this vision, the appropriateness of the current name has come into question. Indeed, a name is representative of the diversity and the leadership of a community, and a two thirds majority of IFOMPT Member Organizations (MO’s) triggered the formation of a task force to investigate this question. Following feedback from IFOMPT MO’s and external organizations, the IFOMPT MO’s will be asked in August 2021 whether or not they support a proposal to change the name of the organization to the International Federation of Musculoskeletal Physical Therapists (IFOMPT). The question is whether it is necessary to change our name to achieve this vision. In our minds, two major reasons exist to support a name change. Firstly, the current name, orthopedic manipulative therapy is not an internationally recognized terminology within the medical, government, non-government or wider community circles. Secondly, the term orthopedic manipulative therapy no longer reflects contemporary practice.
Relationship of Pericardial Fat Tissue With Cardiovascular Risk Factors in Patients Without Cardiovascular Diseases.

Background: Obesity and related cardiovascular diseases (CVDs) are important public health problems. The role of visceral ectopic fat remains contested. We studied the relationship between pericardial fat tissue (PFT) volume and CVD risk factors. Methods: We examined 320 patients (average age 63.8 ± 19.9 years) without manifested CVD. Anthropometric indicators were measured, and body mass index (BMI) was calculated. The total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol (HDL-C), and triglycerides were assessed. Cardiovascular (CV) risk was calculated using the SCORE system. All patients underwent chest computed tomography with detection of PFT volume using specialized semiautomatic software. Results: Among study participants with normal body mass, the PFT volume was 1.95 cm3 [2.1; 3.9], while it was 3.0 cm3 [2.0; 3.7] in overweight patients and 3.6 cm3 [2.7; 4.7] in obese patients (P < 0.001). Patients with hypertension (HTN) also had significantly higher PFT volumes compared with individuals without HTN: 3.1 cm3 [2.3; 4.15] versus 1.8 cm3 [1.0; 2.5] (P < 0.001). Patients with higher CV risk had significantly higher PFT volume, categorized as follows: 1.6 cm3 [1.0; 2.4], low risk; 2.24 cm3 [2.0; 3.1], moderate risk; 3.1 cm3 [2.4; 3.7], high risk; and 3.9 cm3 [3.0; 5.1], very high risk, respectively (P < 0.001). Results of multiple regression demonstrated that waist circumference and HDL-C were significantly associated with PFT volume. Another model revealed a significant association of BMI and PFT volume with the level of CV risk. Conclusions: This study demonstrates the association of PFT volume with the major diagnostic criteria of obesity, HTN, lipid disorders, and CV risk measured by the SCORE system.
Familial factors, diet, and risk of cardiovascular disease: a cohort analysis of the UK Biobank.

BACKGROUND Both diet and familial factors have a major role in the development of cardiovascular disease (CVD). However, it remains unclear whether familial predisposition to CVD modifies the association between dietary factors and CVD. OBJECTIVES The aim was to assess whether the association between diet and CVD varies with familial predisposition to CVD. METHODS In this prospective cohort of the UK Biobank, 462,155 CVD-free participants were included in 2006-2010 and followed for CVD incidence until 2020. Food intake was measured using a short food-frequency questionnaire. Familial predisposition was measured by self-reported family history of CVD and by polygenic risk score (PRS) for CVD based on summary statistics of independent genome-wide association studies. RESULTS During a median follow-up of 11.2 y, 46,164 incident CVD cases were identified. A moderately higher risk of CVD was associated with more frequent processed-meat consumption, with an adjusted HR of 1.07 (95% CI: 1.03, 1.11; highest vs. lowest level). Conversely, intakes of fish, cheese, vegetables, and fruit were each associated with reduced CVD risk [HR (95% CI): 0.92 (0.89, 0.96), 0.90 (0.86, 0.94), 0.98 (0.95, 1.00), and 0.93 (0.89, 0.96), respectively]. Stratification analyses by family history of CVD and by PRS for CVD revealed an inverse association between CVD and intakes of fish and cheese, for both subgroups with and without a familial predisposition to CVD. Notably, while the association between processed-meat intake and CVD was restricted to individuals with a familial predisposition to CVD [e.g., HR: 1.11 (1.05, 1.16) and 1.03 (0.97, 1.10) for with and without a family history, respectively, P-interaction < 0.001], the risk reduction of CVD associated with vegetable and fruit intake was only noted among participants without a CVD familial predisposition [e.g., HR for fruit consumption: 1.00 (0.97, 1.03) and 0.91 (0.87, 0.95), respectively, P < 0.001]. CONCLUSIONS Familial factors modify the association between diet and CVD, underscoring the need for personalized dietary guidelines for CVD prevention.
Presence of ethanol‐sensitive and ethanol‐insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice

Glycine receptors composed of α1 and β subunits are primarily found in the spinal cord and brainstem and are potentiated by ethanol (10–100 mM). However, much less is known about the presence, composition and ethanol sensitivity of these receptors in higher CNS regions. Here, we examined two regions of the brain reward system, the ventral tegmental area (VTA) and the prefrontal cortex (PFC), to determine their glycine receptor subunit composition and sensitivity to ethanol.
Development of biodegradable thin films for efficient, specific and controlled delivery of capecitabine

Cancer is the leading cause of death worldwide. Capecitabine (CP) shows severe side effects because of early metabolism in stomach that affects the normal cells and organs, particularly liver and stomach. In this scope, we report the biocompatible, nontoxic polymeric thin films loaded with anti-cancer drug, CP for target specific, sublingual delivery of CP. Chitosan (CS) and polyvinyl alcohol (PVA) were used as biodegradable polymers alongwith glutaraldehyde (GLA) cross linker. CP-loaded thin films (TFCP1–TFCP5) were fabricated by solvent casting method. The results of Fourier transform infrared spectroscopy confirmed the presence of CP and polymers (CS and PVA) with GLA which binds through hydrogen bonding, and compatibility of drug with different excipients. Thermogravemetric analysis showed that the thin films are highly stable while differential scanning calorimeter thermograms confirmed the complete miscibility/entrapment of CP within PVA/CS thin film matrix. X-ray diffraction patterns revealed the molecular ineractions between CP and polymer matrix. High degree of swelling index of thin films at pH 7.4 was observed in comparison to pH 5.5. CP release studies in acetate (pH 5.5) and phosphate buffer (pH 7.4) showed that the thin films swell and result in drug diffusion faster in phosphate buffer through diffusion governed by Higuchi’s model. Cytotoxicity results displayed that CPTFs killed MCF-7 and T47D (human breast adenocarcinoma) cells more effectively as compared to CP alone. The results of adhesion assay also showed that the PVA and CS both are safe and biocompatible. TFCP1 and TFCP3 thin films efficiently induced the apoptosis as compared to CP alone. The improved ability of TFCP1 and TFCP3 to induce cytotoxicity in MCF-7 cells reflects the potential of these thin films for targeted drug delivery. The CPTFs were stable for 4 months at 4 °C/60% ± 2%RH and 25 °C/70% ± 2%RH. In conclusion, the thin film formulations showed target specific controlled and burst release properties and thus could prove to be effective for human breast cancer treatment.
Effect of Feeding Dried Distiller’s Grains Plus Solubles on Milk Yield and its Composition in Dairy Cattle

In developing countries like India, the increasing cost and decreasing supply of traditional feedstuffs are expected to constrain the future expansion of livestock production. Feed prices have increased sharply due to increase in the feed ingredient prices. The availability of low-priced, high-quality feeds is critical for the expansion of the dairy industry. DDGS is one of residual coproduced from the production of ethanol from grain after fermentation of the starch (Youssef et al., 2013). The non-fermentable components of this process which are rich in essential nutrients such as protein, fat, fiber, International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 03 (2018) Journal homepage: http://www.ijcmas. com
The murburn precepts for cellular ionic homeostasis and electrophysiology

Starting from the basic molecular structure and redox properties of its components, we build a macroscopic cellular electrophysiological model. We first present a murburn purview that could explain ion distribution in bulk‐milieu/membrane‐interface and support the origin of trans‐membrane potential (TMP) in cells. In particular, the discussion focuses on how cells achieve disparity in the distribution of monovalent and divalent cations within (K+ > Na+ > Mg2+ > Ca2+) and outside (Na+ > K+ > Ca2+ > Mg2+). We explore how TMP could vary for resting/graded/action potentials generation and project a model for impulse conduction in neurons. Outcomes based on murburn bioenergetic equilibriums leading to solubilization of ion‐pairs, membrane's permittivity, protein channels' fluxes, and proteins’ innate ability to bind/adsorb ions selectively are projected as the integral rationale. We also provide experimental modalities to ratify the projections.