Miracle-dz/UniFAR-FTUs huggingface dataset:
A Zebrafish Model for a Rare Genetic Disease Reveals a Conserved Role for FBXL3 in the Circadian Clock System

The circadian clock, which drives a wide range of bodily rhythms in synchrony with the day–night cycle, is based on a molecular oscillator that ticks with a period of approximately 24 h. Timed proteasomal degradation of clock components is central to the fine-tuning of the oscillator’s period. FBXL3 is a protein that functions as a substrate-recognition factor in the E3 ubiquitin ligase complex, and was originally shown in mice to mediate degradation of CRY proteins and thus contribute to the mammalian circadian clock mechanism. By exome sequencing, we have identified a FBXL3 mutation in patients with syndromic developmental delay accompanied by morphological abnormalities and intellectual disability, albeit with a normal sleep pattern. We have investigated the function of FBXL3 in the zebrafish, an excellent model to study both vertebrate development and circadian clock function and, like humans, a diurnal species. Loss of fbxl3a function in zebrafish led to disruption of circadian rhythms of promoter activity and mRNA expression as well as locomotor activity and sleep–wake cycles. However, unlike humans, no morphological effects were evident. These findings point to an evolutionary conserved role for FBXL3 in the circadian clock system across vertebrates and to the acquisition of developmental roles in humans.
Promoting Safety Climate Training for Migrant Workers through Non-Technical Skills: A Step Forward to Inclusion

Currently, the number of migrant workers is significantly high and represents a global issue. The impact of the negative aspects of employment and psychosocial risk factors in the workplace on migrants’ health and well-being has been underestimated. This study focused first on a perceptions analysis of the diversity climate, safety climate and experiences of discrimination of migrant workers, and then on testing whether and how the contents and meanings of ad hoc training were transferable and transferred to their workplace. Through a program of 4 phases (pre-screening, intervention, assessment and follow-up) and a series of interviews and focus groups, 24 participants were recruited for the study. Consistent with literature, the findings confirmed a poor perception of diversity and safety climate, and several discrimination experiences. The training outcomes showed an improvement of the individual perception of safety, motivation and self-efficacy. In contrast, the work context is still perceived as closed and constraining, which makes complete transferability hard to achieve. Results are quite encouraging and bear out the need for training and the first positive effects on working conditions and quality of life. This study is one of the first attempts in Italy to integrate the analysis of migrants’ labor situation, training and its evaluation. Future studies are required.
Effect of Dietary Palm Kernel Oil on the Quality, Fatty Acid Profile, and Sensorial Attributes of Young Bull Meat

Lipid supplementation through vegetable oils in diets for ruminants can be a nutritional strategy to increase energy density, manipulate ruminal fermentation and change the physicochemical composition and sensorial properties of meat. This study evaluated the optimal dietary inclusion of palm kernel oil (PKO) for Nellore bulls on meat quality. The diets consisted of 0.0, 11.5, 23.0, and 34.6 g/kg dry matter (DM) PKO levels. PKO inclusion did not influence the centesimal composition, pH, color indices, water holding capacity, cooking loss, or shear force of the beef. There were linear increases in the concentrations of lauric acid (C12:0) and myristic acid (C14:0) in the bull’s meat. However, palmitic acid (C16:0), oleic acid (C18:0), vaccenic acid (t-11–C18:1) and conjugated linoleic acid (CLA), ∑n − 6, ∑n − 3, ∑n − 6/∑n 3, the hypocholesterolemic: hypercholesterolemic ratio of the fatty acid content, and the thrombogenicity index were not affected. There were linear reductions in the oleic acid meat concentration (c-9–C18:1) and elongated enzymatic activity when PKO was added to the bull diet. The atherogenicity index increased linearly due to PKO inclusion in the bull diet. No effect of the inclusion of PKO on meat flavor, perception of tenderness, juiciness, or global acceptance from the sensorial evaluation was recorded. The inclusion of PKO up to 34.6 g/kg DM can be recommended to supplement young bulls with no effects on meat composition and quality characteristics.
Checks and balances of the RTG pathway under arginine deprivation and canavanine exposure in Saccharomyces cerevisiae

Decades of research in Saccharomyces cerevisiae underlie the current dogma of mitochondrial retrograde (RTG) signaling: Rtg2-dependent translocation of the heterodimer Rtg1/Rtg3 from the cytoplasm to the nucleus induces the transcription of RTG-target genes under glutamate starvation or loss of respiration. We previously found that RTG mutants show severe growth inhibition from arginine deprivation and are highly sensitive to canavanine when grown on glucose. Here, we show that on solid media, RTG mutants are also sensitive to thialysine, a toxic lysine analog, although lysine deprivation causes a milder growth defect. Growth on an alternative carbon source restores RTG mutants’ ability to grow without arginine or lysine and improves their tolerance of toxic analogs; deletion of MIG1 affords a similar rescue on glucose and improves canavanine tolerance, except for in rtg2Δ. It is well known that the target of rapamycin (TOR) signaling pathway inhibits the RTG pathway. Batch growth experiments with or without TOR inhibition reveal phenotypic and regulatory differences between RTG mutants. rtg1Δ can sustain simultaneous canavanine exposure and TOR inhibition via rapamycin, but rtg2Δ and rtg3Δ cannot. Surprisingly, our data show that under fermentative lifestyle and arginine deprivation, both RTG signaling and TOR activity are required. This expands the universe of TOR and RTG signaling, suggesting bilateral communication rather than unidirectional RTG regulation by TOR. To the best of our knowledge, this work shows for the first time that Rtg3 activity can be separate from its role as a heterodimer with Rtg1. This work also strongly suggests a specific role for Rtg2 in canavanine tolerance.
TiO2 Nanoparticle-Loaded Poly(NIPA-co-NMA) Fiber Web for the Adsorption and Photocatalytic Degradation of 4-Isopropylphenol

A TiO2 nanoparticle-loaded polymer fiber web was developed as a functional material with the ability to adsorb and photo-catalytically degrade organic pollutants in aquatic media. A linear copolymer of N-isopropylacrylamide (primary component) and N-methylol acrylamide (poly(NIPA-co-NMA)) was prepared, and composite fibers were fabricated by electrospinning a methanol suspension containing the copolymer and commercially available TiO2 nanoparticles. The crosslinking of the polymer via the formation of methylene bridges between NMA units was accomplished by heating, and the fiber morphology was analyzed by electron microscopy. 4-Isopropylphenol generated by the degradation of bisphenol A—one of the endocrine-disrupting chemicals—was used as the model organic pollutant. As poly(NIPA) is a thermosensitive polymer that undergoes hydrophilic/hydrophobic transition in water, the temperature-dependence of the adsorption and photocatalytic degradation of 4-isopropylphenol was investigated. The degradation rate was analyzed using a pseudo-first-order kinetic model to obtain the apparent reaction rate constant, kapp. The enhancement of the photocatalytic degradation rate owing to the adsorption of 4-isopropylphenol onto thermosensitive poly(NIPA)-based fibers is discussed in terms of the ratio of the kapp of the composite fiber to that of unsupported TiO2 nanoparticles. Based on the results, an eco-friendly wastewater treatment process involving periodically alternated adsorption and photocatalytic degradation is proposed.
Researching the Spatial Aspects of the Romani-Hungarian coexistence by the Means of Mental Mapping

In our study we shall analyse the spatial aspects of the Romani-Hungarian coexistence based on the field research results of the village seminar workshop organized by the Szent István University (Gödöllő). We present two different situations: one segregate analysis using Baks as an example, where we find a settlement considered as majority, and a Romani segregate; and provincial ghettoization using Átány as an example, where due to a previous site termination the Romani people dispersed over the whole village area. We conducted surveys in both settlements in order to find out the local residents’ opinion on the development of the value of the inhabited area. Our goal on the one hand is to present the method of ethnicity analysis by mental mapping in rural areas, and on the other in addition to offering methodological practices is to point out a few important takeaways of the spatiality of the RomaniHungarian coexistence based on our actual experiences.
Cattle Egrets (Bubulcus ibis) Control the Population of House Flies (Musca domestica) in Garbage Dumping Sites: A Comparison between Two Garbage Dumping Sites, Kandy, Sri Lanka

House flies spread many communicable diseases such as cholera, dysentery and spread parasitic infections. Therefore, it is important to control house flies at garbage dumping sites as they are a rich source of disease agents. Cattle Egret (Bubulcus ibis) is one of the common wetland birds in Sri Lanka, often seen foraging on flies at garbage dumping sites. Referring to the preliminary observations reported before, two garbage dumping sites were selected from Kandy district. Digana garbage dumping site (7.260 N 80.740 E) was selected as a Cattle Egret occupied site and Panvila garbage dumping site (7.220 N 80.430 E) was selected as the control site where no Cattle Egrets were recorded. The adult house flies (Musca domestica) were counted which random quadrate sampling twice a month for 5 min. Birds were counted from 08:00 to 10:00 hrs by point count method with a binocular. Cattle Egrets, Crows, Common Mynahs, Spotted Doves, Green bee-eaters, House Sparrows, Black Robins, Indian Pond Herons and Asian Palm Swifts were identified as bird species in Digana garbage dumping site and only Common Mynas, Spotted Doves and Asian Palm Swifts were observed in Panvila site. A strong correlation between populations of Cattle Egrets and house flies was observed in the Digana garbage dumping site (<0.05). Since major food item of Cattle Egret is insects, they are attracted to waste dumping site, which provides abundant dipterans maggots at low energy expenditure. However, the house fly population was significantly high in Panvila site than Digana site as Cattle Egrets were not observed in Panvila site. Also In both sites, no correlation was observed between populations of other bird species and population of house flies (0.05<). During breeding season, the Cattle Egrets were observed to be migrating away from the Digana site and were not recorded in late June. No Cattle Egrets in July to September were observed in this site. The number of house flies was clearly increased during this period and with the return of Cattle Egrets after breeding season the numbers of house flies decrease dramatically. The present study shows that there is a strong relationship between population of house flies and Cattle Egret abundance. Keywords: Cattle Egrets, Garbage dumping sites, House flies
Strategies of Tourism Service Providers to Cope with the COVID-19 Pandemic

The situation with the COVID-19 pandemic has a negative impact on the development of tourism. It imposes restrictions on both tourism supply and tourism demand. Government lockdowns travel businesses and, at the same time, restricts the freedom of movement. Thus, the supply and demand sides have to cope with the exogenous shocks. The first evidence of experience and reactions of travel businesses was presented in early March 2020. Data from bookings describes how visitors start to adapt their travel behavior to the exit strategies. Even rural tourism is affected by the worldwide spread crisis. To highlight the impacts on rural tourism, this research focuses on middle and small-sized entrepreneurs (e.g., farmers, winemakers, local private rural museums, local accommodation providers;local providers of hippotherapy) and on local tourist organizations (e. g. DMO, tourist information centers) involved in rural tourism. The aim of this study is to show how they overcame the situation with the COVID-19 pandemic. In other words, the study describes the changes in visitor behavior from the perspective of rural tourism providers. The research is based on a case study of South Moravia and applies both qualitative (a questionnaire survey) and quantitative (interviews) methods. The research data shows that rural tourism providers manage to cope with the government restrictions and tailor their services to new customers“ requirements as well. Besides the loss of incomes, tourism service providers face a decrease in the number of visitors, and thus they suffer a slump in sales. The majority of all rural tourism providers responded that their segment of visitors had changed. More specifically, the absence of foreign visitors was replaced by domestic ones. © 2021. All Rights Reserved.
DEVELOPING WALL CLOCKS AS HEALTH PROMOTION MEDIAFOR HOUSEWIFE TARGET IN THE PREVENTION OF DENGUE HEMORRHAGICFEVER (DHF) IN INDRAMAYU

Up to 2012, Larva-Free Numbers Dengue (ABJ) in Indonesia was still low, as well as in Indramayu , which is still below the target, which has only reached 80 % of the target of 95 % . ABJ target achievement done by the efforts of The Eradication of DHF Mosquito Breeding Place (PSN - DBD). Housewife role in doing PSN-DBD is very important. Health promotion media could encourage housewife in doing PSN - DBD. The aim of this study was to develop clocks as a health promotion media to target housewives in the DHF prevention in Indramayu . This type of research was action research. The population in this study were housewives. For developing wall clocks, purposive sampling was taken by certain criteria of 10 housewives, the data collected and analyzed using qualitative analysis. For testing clocks product sample, sample numbers of 30 housewives were taken, data were collected and analyzed by using quantitative descriptive analysis. This research has produced a wall clock with shapes, images, text, color, sound reminder ( alarm) in accordance with the character of housewives. The trial results of wall clocks as health media promotion for DHF prevention, suggested that there were differences in the housewives PSN-DBD action and differences also in the presence of dengue mosquito larvae in water storages in the home and surounding enviroments before and after the test sample product media. Larger samples and longer duration of intervention needed to test the effectiveness this media. Keywords: Wall Clock, Housewife, DHF Prevention
Building a Sense of History: Folk Art for Early Childhood Learners

This study is a result of working with a first-grade teacher, Ellen Stubblefield, who plans, implements, and evaluates a modified Reggio Emilia approach. She documents students’ learning through visual means. In tandem with a kindergarten teacher, Glenda Watson, early childhood learners question and reflect about their community history and that of Harlem primarily through folk art but also in music, poetry, literature, and architecture. Students inquire about historical events in the Harlem Renaissance and connected it to Hoover, Alabama. They deconstruct art works, replicate their favorites, learn the history of the Harlem Renaissance, map Harlem, write about artists, visit the local museum’s exhibit of folk art and make comparisons to their community. Ultimately, they educated peers and parents about the diversity of the people who made Harlem such a wonderful community. As educators, we learn the most. We see young children can begin historical understanding with an active learning/research approach.
Comparison of Flunixin Meglumine, Meloxicam and Ketoprofen on Mild Visceral Post-Operative Pain in Horses

Simple Summary Pain management following surgical intervention is key. In horses, several anti-inflammatories (flunixin meglumine, meloxicam and ketoprofen) are available for the management of pain following castration. However, their analgesic efficacy remains unclear for mild visceral pain. The aim of this study was to compare the analgesic efficacy of the above-mentioned anti-inflammatory drugs, following a simple surgery (inguinal castration). Horses were administered a randomly assigned anti-inflammatory drug before and after surgery. A pain score was recorded using a previously described pain assessment scale (PASPAS) before administration, and after the first and second administrations by a senior clinician and a veterinary student. Thirty horses were evaluated, and there was no significant effect of the drug administered on the pain score. Horse welfare was not compromised regardless of drug assigned. Horses showed mild pain post-operatively, which decreased significantly within 24 h. Pain scores were significantly different when assessed by a veterinary student and a senior clinician. The authors found that the anti-inflammatory drug studied provided a similar level of analgesia for the management of mild visceral pain in horses, but that the pain scale used is not suitable for junior evaluators or, by extension, owners. Abstract The analgesic efficacy of meloxicam and ketoprofen against equine visceral pain is unclear. The aim of this study was to compare the analgesic efficacy of meloxicam (M) and ketoprofen (K) to flunixin meglumine (F) following inguinal castration. Horses undergoing inguinal castration under general anesthesia were randomly assigned F (1.1 mg/kg), M (0.6 mg/kg) or K (2.2 mg/kg) intravenously two hours pre-operatively and 24 h later. A pain score (out of 31) was recorded blindly by a senior clinician and veterinary student before NSAIDs administration (T0), and after the first (T1) and second (T2) administrations, using a modified post-abdominal surgery pain assessment scale (PASPAS). Pain was classified as mild (score ≤ 7), moderate (score = 8–14) or severe (score > 14). Thirty horses (12 F, 10 M, 8 K) aged 6.2 ± 4.9 years, mostly warmbloods, were included. Horse welfare was not compromised regardless of the drug assigned. There was no statistically significant effect of NSAIDs on pain score. Mean pain scores were significantly higher at T1 than T0 for each NSAID (F: 5.08 ± 2.50 vs. 1.58 ± 1.38 (p < 0.001); M: 4.60 ± 2.32 vs. 1.10 ± 1.20 (p < 0.001); K: 5.25 ± 1.39 vs. 1.50 ± 1.51 (p < 0.0001)) and lower at T2 than T1 for F (2.92 ± 2.423 vs. 5.08 ± 2.50 (p < 0.001)) and M (2.90 ± 1.37 vs. 4.60 ± 2.32 (p < 0.0325)). At T1, senior pain scores were significantly different than for junior (5.56 ± 0.54 vs. 3.22 ± 0.62, p = 0.005). This study indicates that meloxicam and ketoprofen provide a similar level of analgesia to flunixin meglumine for the management of mild visceral pain in horses. PASPAS is not reliable for junior evaluators.
Comment on nhess-2022-12

In this work authors train 6 ML algorithm (random forest) using variables of topography, fire, infraestruture, climate, weather, dryness and fuel moisture, to estimate the probability of fire ignition. They create a training dataset for 4 different combinations human/lightning in native/matrix vegetation. The fire ignition data is obtained from Victorian Country Fire Authority, which uses 20 classes for causes of ignition. Then the authors reclassify the ignitions between human or lightning to create the training dataset for the 6 models. In the lightning case, there is the need to create add random fires to create absence data. Then the authors analyse the classification error and the R2. Following, the analyse the importance of the variables for the different models using the decrease of the gini index.
Phytochemical Investigation of Pilea pumila (Clearweed), Laportea canadensis (Wood Nettle), and Boehmeria cylindrica (False Nettle): Three Members of the Urticaceae Family

Phytochemical analysis of 3 members of the Urticaceae family (eg Pilea pumila, Laportea canadensis, and Boehmeria cylindrica) was conducted using GC-MS. P pumila and L canadensis have several recorded historical medicinal uses by Native American tribes, whereas B cylindrica does not. This study was conducted to understand the historical uses in terms of chemical composition. Plant material, separated into aerial or root portions, was dried and extracted using either ethanol or heptane at room temperature. Sterols, fatty acids and esters, terpenes and terpenoids, saturated and monounsaturated hydrocarbons, small oxygen-functionalized organic molecules, and tocopherols were observed using these methods. Plant sterols composed 30.3% to 62.2% of the observed extracts, followed by fatty acids and esters ranging from 0% to 49% and terpenes and terpenoids ranging 4.56% to 33.9%. All 3 plants contained γ-sitosterol as the dominant plant sterol and palmitic, linoleic, and linolenic acids and esters. The 3 plants differed significantly in the observed terpene and terpenoid composition. Germacrene D-4-ol, known to have antifungal activity, was the major terpenoid identified in P pumila (7.42%-7.76%), phytol (4.13%), α-cadinene (3.96%), and cadina-1,4-diene (5.53%) were observed in L canadensis treatments, and squalene (2.20%-7.04%), phytol (4.17%), and cadina-1,4-diene (6.68%) were observed in B cylindrica treatments. Possible rationales for Native American medicinal uses were evaluated.
Reflections on the Professional Talents Cultivation Project under the Condition of Credit System

As a systematic project of the credit system,the establishment of professional talents cultivation project is very crucial.In the process of establishment of the project,we must fully understand the meaning of the credit system,comprehensively research and investigate the key elements such as teachers,curriculum resources,etc.The project should be scientific and rational,which can demonstrate the professional qualities.