Miracle-dz/UniFAR-FTUs huggingface dataset:
Value of Care: An Exploratory Qualitative Study with Doctors and Patients

The concept of value in healthcare is mainly based on economic and financial aspects. However, the literature has emphasised the need to investigate value from other perspectives. The present study aimed to explore the views of physicians and patients on the value of healthcare, and to examine in depth the psychosocial and organisational elements that have emerged but that need to be investigated more. Therefore, two qualitative studies were performed, in which 69 physicians and 111 patients participated. The data were analysed using content analysis and text mining using t-lab software. The results revealed common elements between the two healthcare actors that constitute value in healthcare, including competence, professionalism, and soft skills like communication and empathy. Furthermore, the importance of functioning health services and effective organisational culture in local healthcare and investment emerged. These findings can guide healthcare organisations to consider the potential psychosocial factors related to value in healthcare, which affect organisation in terms of costs and healthcare relationships. In addition, these findings are a first step in filling the gap found in the literature regarding the consideration of value from a non-economic perspective and the difficulty of defining and measuring it.
Involvement of a MYB Transcription Factor in Anthocyanin Biosynthesis during Chinese Bayberry (Morella rubra) Fruit Ripening

Simple Summary Color is one of the most significant quality characteristics of Chinese bayberry (Morella rubra). Exploration of the anthocyanin biosynthesis mechanism is crucial for the genetic improvement of color quality. Many MYB transcription factors are reported to control the biosynthesis of anthocyanins in a variety of plant species. However, it is still unclear how the MYB genes in Chinese bayberry contribute to the synthesis of anthocyanins. In this research, we isolated the MYB transcription factor, MrMYB9, based on comparative transcriptome analysis of red and white Chinese bayberry fruits. We also profiled MrMYB9 gene expression during the maturation period and in specific tissues. Through bioinformatics and molecular biology experiments, MrMYB9 was subsequently identified as a transcription factor of the R2R3-MYB type associated with anthocyanin biosynthesis. Our research sheds insights on the biosynthesis of anthocyanins during color maturation in the Chinese bayberry. Abstract Anthocyanin is a class of water-soluble flavonoids found in Chinese bayberry (Morella rubra) that is not only responsible for the variety of colors visible in nature but also has numerous health-promoting benefits in humans. Through comparative transcriptomics, we isolated and identified a transcription factor (TF) of the R2R3-MYB type, MrMYB9, in order to explore the anthocyanin biosynthesis pathway in red and white Chinese bayberries. MrMYB9 transcript was positively correlated with anthocyanin level and anthocyanin biosynthetic gene expression during Chinese bayberry fruit maturation (R-values in the range 0.54–0.84, p < 0.05). Sequence analysis revealed that MrMYB9 shared a similar R2R3 domain with MYB activators of anthocyanin biosynthesis in other plants. MrMYB9 substantially transactivated promoters of anthocyanin biosynthesis-related EBGs (MrCHI, MrF3’H, and MrANS) and LBGs (MrUFGT) upon co-expression of the AtEGL3 gene. Our findings indicated that MrMYB9 may positively modulate anthocyanin accumulation in Chinese bayberry.
Undergraduate Interprofessional Education in the European Higher Education Area: A Systematic Review

Background: As a contribution to developing interprofessional education (IPE) synergy between medical education systems in the European Higher Education Area (EHEA), this review aims to describe the IPE experiences for undergraduate medical students implemented in EHEA member countries. Methods: This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The search was conducted on SCOUPS and MEDLINE databases. Inclusion criteria were peer-reviewed English language articles about undergraduate medical students, interprofessional education, and EHEA countries, published January 2000–September 2022. Results: The 32 included studies were from 14 of the 49 EHEA countries. In most of the studies, the theoretical background leading the intervention was not reported (n = 25), and in several studies (n = 16) the students were from two professions only. The reported outcomes were related to self-assessment knowledge about IPE and satisfaction about the program. In 24 studies, the assessment was based on the study’s ad hoc measures only. Limitations ranged from selection bias to lack of objective measures. Conclusion: Future directions should envision developing IPE among EHEA countries, including agreement and consistency across EHEA countries in reporting theories, educational methods, and standardized IPE evaluation measures.
High-Resolution Magic Angle Spinning (HRMAS) NMR Identifies Oxidative Stress and Impairment of Energy Metabolism by Zearalenone in Embryonic Stages of Zebrafish (Danio rerio), Olive Flounder (Paralichthys olivaceus) and Yellowtail Snapper (Ocyurus chrysurus)

Zearalenone (ZEA) is a mycotoxin, commonly found in agricultural products, linked to adverse health impacts in humans and livestock. However, less is known regarding effects on fish as both ecological receptors and economically relevant “receptors” through contamination of aquaculture feeds. In the present study, a metabolomics approach utilizing high-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) was applied to intact embryos of zebrafish (Danio rerio), and two marine fish species, olive flounder (Paralichthys olivaceus) and yellowtail snapper (Ocyurus chrysurus), to investigate the biochemical pathways altered by ZEA exposure. Following the assessment of embryotoxicity, metabolic profiling of embryos exposed to sub-lethal concentrations showed significant overlap between the three species and, specifically, identified metabolites linked to hepatocytes, oxidative stress, membrane disruption, mitochondrial dysfunction, and impaired energy metabolism. These findings were further supported by analyses of tissue-specific production of reactive oxygen species (ROS) and lipidomics profiling and enabled an integrated model of ZEA toxicity in the early life stages of marine and freshwater fish species. The metabolic pathways and targets identified may, furthermore, serve as potential biomarkers for monitoring ZEA exposure and effects in fish in relation to ecotoxicology and aquaculture.
Antibacterial Activities and Life Cycle Stages of Asparagopsis armata: Implications of the Metabolome and Microbiome

The red alga Asparagopsis armata is a species with a haplodiplophasic life cycle alternating between morphologically distinct stages. The species is known for its various biological activities linked to the production of halogenated compounds, which are described as having several roles for the algae such as the control of epiphytic bacterial communities. Several studies have reported differences in targeted halogenated compounds (using gas chromatography–mass spectrometry analysis (GC-MS)) and antibacterial activities between the tetrasporophyte and the gametophyte stages. To enlarge this picture, we analysed the metabolome (using liquid chromatography–mass spectrometry (LC-MS)), the antibacterial activity and the bacterial communities associated with several stages of the life cycle of A. armata: gametophytes, tetrasporophytes and female gametophytes with developed cystocarps. Our results revealed that the relative abundance of several halogenated molecules including dibromoacetic acid and some more halogenated molecules fluctuated depending on the different stages of the algae. The antibacterial activity of the tetrasporophyte extract was significantly higher than that of the extracts of the other two stages. Several highly halogenated compounds, which discriminate algal stages, were identified as candidate molecules responsible for the observed variation in antibacterial activity. The tetrasporophyte also harboured a significantly higher specific bacterial diversity, which is associated with a different bacterial community composition than the other two stages. This study provides elements that could help in understanding the processes that take place throughout the life cycle of A. armata with different potential energy investments between the development of reproductive elements, the production of halogenated molecules and the dynamics of bacterial communities.
Astrophysical Constraints on Multidimensional Primordial Black Holes based on Neutron Stars Absorption

Proposed first by Ya. B. Zeldovich and I. D. Novikov [1], Primordial Black Holes have become a very effective tool for the early Universe study [2]. These fundamental objects appear as a consequence of basic cosmological principles and carry information about the cosmological stages inaccessible by other means. A discovery that our Universe can be a submanifold–"brane" embedded in a multidimensional bulk with even noncompact and infinite extra dimensions opens up a new perspective both for mini black holes production in particle collisions and for stability of celestial bodies with respect to absorption them by multidimensional black hole. Different aspects of the last question were considered in [3] using non relativistic approximation. In [4] consequences of a White Dwarf (WD) absorption by brane PBH is considered. Now we are interested in absorption of a Neutron Star (NS), thus one needs to consider a relativistic generalization of the equations for accretion on D-dimensional PBH. Since matter is localized on the brane, the regular conservation equations for the energy– momentum tensor ∇νT μν = 0 and baryons number ∇ν(nu ) = 0 can be used for a neutral and non rotating D-dimensional black hole with
Diagnosis of Periprosthetic Joint Infection: The Utility of Biomarkers in 2023

Periprosthetic joint infection (PJI) is a rare yet devastating complication following total joint arthroplasty (TJA). Early and accurate diagnosis of PJI is paramount in order to maximize the chances of successful treatment. However, we are yet to identify a single “gold standard” test for the diagnosis of PJI. As a result, the diagnosis of PJI is often challenging. Currently, the 2018 ICM definition of PJI is the only validated diagnostic criteria available. This article will review the importance of serum and synovial biomarkers in the diagnosis of PJI. In addition, it will provide a brief overview of the emerging modalities for the identification of infections in this setting.
Optimization of the Core Compound for Ytterbium Ultra-Short Cavity Fiber Lasers

Highly ytterbium-, aluminum- and phosphorus-co-doped silica fibers with low optical losses were fabricated by the MCVD method, utilizing an all-gas-phase deposition technique. Optical and laser properties of the active fibers with a phosphosilicate and aluminophosphosilicate glass cores doped with 1.85 mol% and 1.27 mol% Yb2O3 were thoroughly investigated. With the help of hydrogen loading, it was possible to induce highly reflective Bragg grating in both fiber samples using the standard phase-mask technique and 193 nm-UV laser irradiation. The ultra-short (less than 2 cm long) Fabry–Perot laser cavities were fabricated by inscribing two fiber Bragg gratings (highly and partially reflective FBGs) directly in the core of the fiber samples. The highest pump-to-signal conversion efficiency of 47% was demonstrated in such laser configuration using phosphosilicate fiber. The reasons for the low efficiency of aluminophosphosilicate fiber are discussed.
No Media, No Voters? The Relationship between News Deserts and Voting Abstention

Local journalism has suffered major transformations as traditional business models collapse and habits of news consumption change. A lack of funding and successive economic crises have brought about, on a global scale, the shutdown of many news outlets in smaller territories. These areas are becoming “news deserts”, a phenomenon that has been mapped in Brazil and Portugal. Territories without news could see an uptick in social problems such as disinformation, populism, and democratic crises, especially because of voting abstention. Background: This paper aims to analyze the relationship between news deserts and democracy, focusing on how news deserts correlate with voting abstention rates in Brazil and Portugal. Methods: A literature review was carried out including data from news deserts in both countries. The abstention rates in this analysis concern national elections held in 2022. A correlation analysis using binary logistic regression was deployed comparing municipalities with the highest and the lowest abstention rates. Results: In both countries, it was not possible to assess whether there was a correlation between abstention rates and the existence of news deserts. Conclusions: While the absence of media outlets is not correlated with the mobilization of citizens to vote, other variables may be affecting voters’ abstention behaviors.
Monetary Compensation and Private Information Sharing in Augmented Reality Applications

This research studied people’s responses to requests that ask for accessing their personal information when using augmented reality (AR) technology. AR is a new technology that superimposes digital information onto the real world, creating a unique user experience. As such, AR is often associated with the collection and use of personal information, which may lead to significant privacy concerns. To investigate these potential concerns, we adopted an experimental approach and examined people’s actual responses to real-world requests for various types of personal information while using a designated AR application on their personal smartphones. Our results indicate that the majority (57%) of people are willing to share sensitive personal information with an unknown third party without any compensation other than using the application. Moreover, there is variability in the individuals’ willingness to allow access to various kinds of personal information. For example, while 75% of participants were open to granting access to their microphone, only 35% of participants agreed to allow access to their contacts. Lastly, monetary compensation is linked with an increased willingness to share personal information. When no compensation was offered, only 35% of the participants agreed to grant access to their contacts, but when a low compensation was offered, 57.5% of the participants agreed. These findings combine to suggest several practical implications for the development and distribution of AR technologies.
Nontrivial twisted Alexander polynomials

In this note I study the twisted Alexander polynomial of a hyperbolic knot, twisted by a lift of the holonomy representation in SL2(C). Dunfield, Friedl, and Jackson conjectured that its degree is 4g − 2, where g is the genus of the knot. Here I prove that its degree is at least 2, hence it is nontrivial. 2010 Mathematics Subject Classification: 57M25, 57M27.
Methylammonium Tetrel Halide Perovskite Ion Pairs and Their Dimers: The Interplay between the Hydrogen-, Pnictogen- and Tetrel-Bonding Interactions

The structural stability of the extensively studied organic–inorganic hybrid methylammonium tetrel halide perovskite semiconductors, MATtX3 (MA = CH3NH3+; Tt = Ge, Sn, Pb; X = Cl, Br, I), arises as a result of non-covalent interactions between an organic cation (CH3NH3+) and an inorganic anion (TtX3−). However, the basic understanding of the underlying chemical bonding interactions in these systems that link the ionic moieties together in complex configurations is still limited. In this study, ion pair models constituting the organic and inorganic ions were regarded as the repeating units of periodic crystal systems and density functional theory simulations were performed to elucidate the nature of the non-covalent interactions between them. It is demonstrated that not only the charge-assisted N–H···X and C–H···X hydrogen bonds but also the C–N···X pnictogen bonds interact to stabilize the ion pairs and to define their geometries in the gas phase. Similar interactions are also responsible for the formation of crystalline MATtX3 in the low-temperature phase, some of which have been delineated in previous studies. In contrast, the Tt···X tetrel bonding interactions, which are hidden as coordinate bonds in the crystals, play a vital role in holding the inorganic anionic moieties (TtX3−) together. We have demonstrated that each Tt in each [CH3NH3+•TtX3−] ion pair has the capacity to donate three tetrel (σ-hole) bonds to the halides of three nearest neighbor TtX3− units, thus causing the emergence of an infinite array of 3D TtX64− octahedra in the crystalline phase. The TtX44− octahedra are corner-shared to form cage-like inorganic frameworks that host the organic cation, leading to the formation of functional tetrel halide perovskite materials that have outstanding optoelectronic properties in the solid state. We harnessed the results using the quantum theory of atoms in molecules, natural bond orbital, molecular electrostatic surface potential and independent gradient models to validate these conclusions.
“Together against Tuberculosis”: Cascade of Care of Patients Referred by the Private Health Care Providers in the Kyrgyz Republic

Until 2021, in the Kyrgyz Republic, tuberculosis (TB) was diagnosed and treated only in the public sector. With funding support of the STOP–TB partnership, the private providers in four regions of the country and Bishkek city were mapped, trained and incentivized to screen for and identify presumptive TB patients and refer them to the public facilities for diagnosis and treatment. In this study, we describe the cascade of care of such patients. This was a cohort study involving secondary analysis of routine data. Of 79,352 patients screened during February 2021–March 2022, 2511 (3%) had presumptive TB, of whom 903 (36%) were not tested for TB [pre-diagnostic loss to follow-up]. A total of 323 (13%) patients were diagnosed with TB, of whom, 42 (13%) were not started on treatment [pre-treatment loss to follow-up]. Among 257 patients eligible for outcome assessment, 197 (77%) had treatment success, 29 (11%) were lost-to-follow-up, 13 (5%) died, 4 (2%) had treatment failure and 14 (5%) were not evaluated. While this donor-funded, pioneering initiative was successful in engaging the private sector, we recommend that the national TB programme scales up the initiative nationally with dedicated budgets, activities and plans to monitor progress. Qualitative research is urgently needed to understand the reasons for the gaps in the care cascade.
New Advances and Perspectives of Influenza Prevention: Current State of the Art

The modern world, swaddled in the benefits of civilization, has fostered the development of science and the introduction of products of technological progress. This has allowed serious individual health problems, including those associated with viral diseases, to become targets for prophylaxis, treatment, and even cure. Human immunodeficiency viruses, hepatitis viruses, coronaviruses, and influenza viruses are among the most disturbing infectious agents in the human experience. Influenza appears to be one of the oldest viruses known to man; these viruses were among the first to cause major epidemics and pandemics in human history, collectively causing up to 0.5 million deaths worldwide each year. The main problem in the fight against influenza viruses is that they mutate constantly, which leads to molecular changes in antigens, including outer membrane glycoproteins, which play a critical role in the creation of modern vaccines. Due to the constant microevolution of the virus, influenza vaccine formulas have to be reviewed and improved every year. Today, flu vaccines represent an eternal molecular race between a person and a virus, which neither entity seems likely to win.
Straw Biochar-Facilitated Methanogenesis from Acetic Acid and Ethanol: Correlation with Electron Exchange Capacity

Straw biochar prepared by three methods (i.e., pyrochar, HNO3-modified pyrochar, and hydrochar) was added to the anaerobic digestion system with acetic acid and ethanol as substrates to explore the effects of biochar on methane production, substrate degradation, and microbial community structure. The biogas yields of the biochar-supplemented groups all increased, and the maximum methane yield was found in the hydrochar group, which was 45.4% higher than the control. In the ethanol-fed reactor, the maximum partial pressure of hydrogen in the headspace of the hydrochar reactor was reduced from 3.5% (blank reactor) to 1.9%. Overall, methane production is directly proportional to the electron exchange capacity (EEC) value of biochar. Furthermore, the bio-aging process increased the EEC of each kind of biochar to 5.5–8.1%, which was favorable for the sustainable promotion of methanogenesis. The increased methane yield from the bio-aged biochar could either be attributable to the changes in surface oxygen-containing functional groups or the selectively enriched microbial community on the biochar, such as Geobacter, which could participate in direct interspecies electron transfer.
Wild Fruits of Crataegus monogyna Jacq. and Sorbus aria (L.) Crantz: From Traditional Foods to Innovative Sources of Pigments and Antioxidant Ingredients for Food Products

Hawthorn (Crataegus monogyna Jacq.) and whitebeam (Sorbus aria (L.) Crantz) are wild species traditionally used as ethnic foods in the Mediterranean area. Their red berries, and mainly the peels, may be used as ingredients due to their color (replacing other synthetic colorants) or functional properties. Some previous studies analyze all edible fruits, but there is very little literature on the composition and properties of the pulpless epidermis of the fruits of C. monogyna and no literature concerning the fruits of S. aria. Total phenolic compounds (TPC) and families of hydroxybenzoic acids, hydroxycinnamic acids, flavonols, and total monomeric anthocyanins were determined in the epidermis of C. monogyna and S. aria fruits. The in vitro antioxidant capacity was also determined using QUENCHER (Quick-Easy-New-CHEap-Reproducible) methodology. Anthocyanins profiles were analyzed in hydroalcoholic extracts through HPLC/MS. C. monogyna fruits presented higher content of TPC than S. aria, with hydroxybenzoic acids (2870.6 mg GAE/100g dw) as the major family, followed by flavonols (771.4 mg QE/100 g dw) and hydroxycinnamic acids (610.3 FAE/100 g dw). Anthocyanins were found in 251.7 mg cyanidin-3-glucoside/100 g dw, characterized by the content of cyanidin-O-hexoxide and peonidin-O-hexoxide. The levels of these compounds correlated with higher values of a* parameter (higher intensity of reddish color). These fruits also showed higher antioxidant capacity by Q-Folin–Ciocalteu and Q-FRAP. S. aria peels had fewer phenolic compounds, particularly anthocyanins (33.7 mg cyanidin-3-glucoside/100 g dw), containing different cyanidin derivatives. From these results, new insights about the composition of the epidermis of these wild fruits are provided, and their potential as ingredients for the food industry is corroborated.