Miracle-dz/UniFAR-FTUs huggingface dataset:
Engaging nurse students in a meaningful English learning: Step by step of nursing simulation technique

English for Specific Purposes, which has been so-called ESP, created to achieve specific learning goals for specific students in a specific field. Therefore, an ESP instructor is required to find specific teaching techniques in order to meet students’ learning needs. This in-practice article reports the implementation of step by step of teaching nursing English through a simulation technique. This study attempted to examine the impact of this teaching technique on the improvement of students’ language skills. There have been abundant resources of research findings in the field of English for Specific Purposes. However, there is still little known study in the field of nursing English, particularly, the investigation of the impact of nursing simulation techniques on nurse students’ language learning achievement. To fill this void, the current study invited 58 nurse students to participate in this research. The simulation teaching technique was introduced to a nursing English class. After that, students’ responses were documented through questionnaires and interviews. The data indicated that there has been positive attitude of the students toward this teaching technique even though time allocation and students’ language proficiency remain problematic to the language learners. The implication of this research may expand the research findings in the field of TESOL Methodology, particularly, English for Nursing.
Technological and microbiological factors affecting the polyphenolic profile of Montenegrin red wines

The main objective of this study was to determine the impact of three commercial yeasts (BDX, ICVD21 and BM4x4) and lactic acid bacteria (VP41 and ALPHA) on the phenolic profile of Montenegrin red wines. Wines made of two autochthonous grape varieties (Vranac and Kratosija) and international grape variety (Cabernet Sauvignon) were examined. The research was performed during the 2012 and 2013 vintage and twenty-one phenolic compounds, including stilbenes, hydroxycinnamic and hydroxybenzoic acids, and flavonols have been identified in the wine after alcoholic and malolactic fermentation, using the liquid chromatography-mass spectrophotometry method. The influence of different commercial yeasts on all analysed phenolic compounds was statistically significant (p ? 0.05) for all examined wines, and there were also statistically significant differences between varietal wines. After alcoholic and malolactic fermentation, the highest average content of p-coumaric acid was determined in Vranac wines. Additional analysis of stilbenes after alcoholic fermentation of varietal wines of the 2013 vintage revealed that the total content of stilbenes was the highest in Vranac wines, where a significant increase of their concentration was observed in wines inoculated with commercial yeast. A similar trend was observed in all studied wine varieties.
High‐Performance Electrocatalytic Conversion of N2 to NH3 Using Oxygen‐Vacancy‐Rich TiO2 In Situ Grown on Ti3C2Tx MXene

To achieve the energy‐effective ammonia (NH3) production via the ambient‐condition electrochemical N2 reduction reaction (NRR), it is vital to ingeniously design an efficient electrocatalyst assembling the features of abundant surface deficiency, good dispersibility, high conductivity, and large surface specific area (SSA) via a simple way. Inspired by the fact that the MXene contains thermodynamically metastable marginal transition metal atoms, the oxygen‐vacancy‐rich TiO2 nanoparticles (NPs) in situ grown on the Ti3C2Tx nanosheets (TiO2/Ti3C2Tx) are prepared via a one‐step ethanol‐thermal treatment of the Ti3C2Tx MXene. The oxygen vacancies act as the main active sites for the NH3 synthesis. The highly conductive interior untreated Ti3C2Tx nanosheets could not only facilitate the electron transport but also avoid the self‐aggregation of the TiO2 NPs. Meanwhile, the TiO2 NPs generation could enhance the SSA of the Ti3C2Tx in return. Accordingly, the as‐prepared electrocatalyst exhibits an NH3 yield of 32.17 µg h−1 mg−1cat. at −0.55 V versus reversible hydrogen electrode (RHE) and a remarkable Faradaic efficiency of 16.07% at −0.45 V versus RHE in 0.1 m HCl, placing it as one of the most promising NRR electrocatalysts. Moreover, the density functional theory calculations confirm the lowest NRR energy barrier (0.40 eV) of TiO2 (101)/Ti3C2Tx compared with Ti3C2Tx or TiO2 (101) alone.
Manufacture of Nutraceutical Compounds from Chestnut Shells by Hydrothermal Processing

The depletion of the fossil resources entails that the modern society must face two important challenges: the substitution of fossil-based products by biomass-based ones and the change from a linear economy towards a circular model, which could allow establishing a global sustainable bio-economy. To accomplish these great challenges, in the last decades the employment of by-products and residues from the agro-food industry to manufacture added-value products has been explored. This would constitute an important step towards economically sustainable and environmentally friendly industrial processes. In this scenario, waste products of lignocellulosic nature represent an attractive, inexpensive and renewable feedstock for the manufacture of biofuels, energy, chemicals and polymers. Chestnut shells are rejected lignocellulosic residues from the agro-food industry with promising possibilities as a suitable feedstock to be used under a biorefinery perspective. These by-products have been employed as a source of antioxidants, mono- and oligosaccharides and to prepare adsorbents for pollutants removal. The development of alternative applications for the chestnut shells could contribute to the integral valorisation of their main constituents. In this work, the hydrothermal treatment of chestnut shells was proposed with a double purpose: to assess its suitability for the solubilisation of the hemicellulosic fraction and phenolic compounds and to determine the optimal conditions that allow the obtaining of the maximum concentration of hemicellulosic oligosaccharides with the minimum amount of monosaccharides and degradation products in the liquid phase. The yield in hemicellulosic oligosaccharides and phenolic compounds is a key parameter for the economic evaluation of the hydrothermal process. This process would permit a selective fractionation of the feedstock as it is designed to solubilise a high percentage of hemicelluloses and phenolic compounds leaving a solid enriched in cellulose and lignin. The obtained results showed that the temperature that enabled the obtaining of the highest concentration of total oligosaccharides and the maximum total phenolic compounds was 180 oC.
Projected impacts of 1.5 and 2°C global warming on temperature and precipitation patterns in South America

Even if the thresholds set out in the Paris Agreement are reached, several impacts on the climate system are still inevitable, generating new vulnerabilities and amplifying those that already exist. In this sense, the objective of this work was to analyse the impact of a global warming of 1.5 and 2°C in the regional climatological patterns of the near‐surface air temperature and precipitation over South America, locating the most affected regions and briefly discussing the possible impacts to be faced on biodiversity and agriculture. The simulations and projections of 26 General Circulation Models (GCMs) from the Coupled Model Intercomparison Project Phase 5 were used, forced in four different Representative Concentration Pathways (RCPs) scenarios. Projections for 1.5 or 2°C global warming indicate a local increase even higher, of at least +0.5°C, in almost the entire South American continent. Regarding precipitation, a similar pattern was also found between the two thresholds of global warming. GCMs project an increase of about 100 mm year−1 in the southern region of Brazil and in the northern portion of the Brazilian Northeast, in northern Argentina, Uruguay, and parts of Peru, Ecuador, Colombia and Venezuela; while the areas between the south of Chile and Argentina and the extreme north of South America show reductions of up to 150 mm year−1. In addition, both the temperature and precipitation patterns were practically similar between the four analysed RCPs scenarios. The results presented in this study indicate that even if the Paris Agreement is very successful, South America will still suffer several impacts and will need to take effective adaptation measures in the short term. This may have pervasive implications for the biodiversity and genetic resource base of the subcontinent, as well as may impair agricultural productivity or incur into considerable adaptation costs for the sector.
A Trust Based Smart City Adoption Model for the Australian Regional Cities: A Conceptual Framework

With nearly half of the world’s population living in the cities, many city and local governments are seeking to deploy smart solutions to their everyday city operations through the implementation of smart city services. However, the subject of smart city services has always been associated with trustworthiness of the services by its users due to security and privacy concerns. These issues may have a major impact on the smart city services adoption. The aim of this proposed research is to examine the technology, organisational, environment, and security determinants that influence stakeholders’ trust towards their intention to adopt smart city services in Australian regional cities. For this, Technology-Organisation-Environment framework together with security related factors for ensuring stakeholders’ trust will be tested using both quantitative and qualitative data. Structural equation modelling technique will be carried out using Smart PLS to test the presented hypotheses and the results will be finally discussed.
Interfacial Modification of Sol-Gel ZnO/AZO Bilayer as Highly Efficient Electron Transport Layer for Perovskite Solar Cells

We report an interfacial modification of sol-gel ZnO/A1-doped ZnO (AZO) bilayer thin film as the electron transport layer (ETL) in the perovskite solar cells. We found that the power conversion efficiency (PCE) of perovskite solar cells was significantly increased from 11.28% to 14.24% with short-circuit current density (J sc ) of 19.53 rnA cm−2,open-circuit voltage (V oc ) of 1.12 V, and fill factor (FF) of 65.1% by employing the AZO thin film as the buffer layer. Moreover, the enhancement in performance was obtained through energy-level offset adjustment of AZO thin film, which allows the better charge separation of perovskite solar cells.
Dealuminated Beta Zeolite Modified by Alkaline Earth Metals

Alkaline Earth metals (Mg, Sr, and Ca) were incorporated into the dealuminated mesoporous beta zeolite (DeAlBeta) by the two-step postsynthesis method. Physicochemical properties of both unmodified and alkaline Earth metal-modified DeAlBeta zeolite were characterized by XRD, DR UV-vis, FTIR, TPD of NH3 and CO2, NMR, and XPS. The dealumination of beta zeolite led to decrease of its acidity and basicity. The incorporation of alkaline Earth metals into the framework of dealuminated beta zeolite did not affect its structure. The modification of DeAlBeta with a small amount of alkaline Earth metals increases the number of acidic centers, which may be related to the formation of framework Mg (Ca or Sr) (II) Lewis acidic sites.
Processing methods for high-amylase corn: impact on ruminal digestion and feedlot cattle performance

Three studies evaluated effects of high-amylase corn (Enogen Feed Corn, EFC) on ruminal digestive characteristics using different processing methods; a 4 study evaluated performance of finishing cattle fed steam-flaked EFC. In study 1, mill-run corn (CON) and EFC were ground through 9-, 6-, or 4 -mm screens, blended to contain 0, 33.3, 66.7, or 100% EFC, and heated to 50, 75, or 1000C (plus a non-heated control). No 2or 3-way interactions occurred (P > 0.05). Increasing EFC in blends linearly improved in situ dry matter disappearance (ISDMD; P < 0.01) and in vitro gas production (IVGP; P = 0.02). Study 2 used blends of EFC and CON containing 0, 25, 50, 75, or 100% EFC, which were reconstituted to 27, 30, or 33% moisture, and ensiled. High-amylase corn did not affect ISDMD (P = 0.19) but IVGP increased linearly (P < 0.01) in response to greater amounts of EFC. Acetate:propionate ratio and total volatile fatty acid (VFA) production by in vitro fermentation improved linearly by increasing EFC (P < 0.02). Study 3 used the same blends of EFC and CON as study 2. Grains were tempered with 0, 3, or 6% moisture, steam conditioned 15, 30, or 45 min, and flaked. No 2or 3-way interactions occurred. Starch availability, ISDMD, IVGP, and in vitro production of acetate, propionate, and total VFA increased linearly (P < 0.01) with greater flaked EFC in blends. Study 4 fed finishing diets consisting of steam-flaked CON, or EFC, for 136 d to 700 crossbred beef heifers (394 ± 8.5 kg initial BW). Compared to CON, cattle fed EFC had similar DMI (P = 0.78) but had greater ADG (P < 0.01) and a 5% improvement in feed efficiency (P < 0.01). Carcass weight was 6 kg greater for EFC cattle (P < 0.01), which also had 8% fewer liver abscesses (P = 0.03) than CON. Marbling score was greater in CON cattle (P = 0.04) than EFC; no differences in USDA Quality Grade (P = 0.33), Yield Grade (P = 0.13), LM area (P = 0.89), or 12-rib fat thickness (P = 0.21) were evident. Improvements in digestion associated with EFC are likely confined to that component of grain mixtures due to a lack of quadratic effects. Improvements in feed efficiency, carcass weight, and potential liver abscess mitigation may be of advantageous use for producers.
Ultrasound one pot synthesis of fused quinazolinones and quinazolinediones, screening and molecular docking study as phosphodiesterase 7A inhibitors.

Phosphodiesterase 7A enzyme is one the most recent targets for designing potent anti-inflammatory agents with minimal side effects. Quinazolinones are unique building blocks of wide biological activities. Different Quinazolinones were reported to act as Phosphodiesterase 7A inhibitors anti-inflammatory particularly against choronic inflammation and autoimmune disease. Ultrasound is a green convenient method for synthesis of different heterocyclic ring system that is advantageous in terms of yield and reaction time. Ultrasound was used for one pot synthesis of 3-substituted 6-aryl-dihydroisoindolo[2,1-a]quinazoline-5,11-diones and 3-Aryl quinazoline-2,4(1H,3H)-diones. Seventeen compounds were synthesized in good yields. The synthesized compounds were inspected for in vitro inhibitory activity against phosphodiesterase7A enzyme. Molecular docking was used to study the mode of interaction of all the synthesized compounds into the enzyme phosphodiesterase 7A binding site. Five compounds showed high inhibitory activity of enzyme Phosphodiesterase 7A at micro-molar level compared to reference drug. The compounds showed good recognition at the enzyme binding site in the molecular docking. There was a good agreement between the molecular docking and the biological screening results.
Biosurfactant As the Next Antimicrobial Agents in Pharmaceutical Applications

The number of patients with antimicrobial resistance is growing as a result of new emerging microbes or overuse of antibiotics. A new substitute to the existing antimicrobial agents is important in time to come to control the mortality rate in the global population. Natural substances, like biosurfactants or commonly known as microbial surfactants could be a potential antimicrobial agent to medical personnel’s consideration as some biosurfactants exhibits antimicrobial activity. Hence, this paper will briefly highlight some of the findings from contemporary researchers who have tested different biosurfactants for potential antimicrobial activity.
Effect of Different Cooking Methods on the True Retention of Vitamins, Minerals, and Bioactive Compounds in Shiitake Mushrooms (Lentinula edodes)

Graduate School of Agricultural Science, Hokkaido University, Kita-9, Nishi-9, Kika-ku, Sapporo, 060-8589, Japan Division of Food and Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 28644, Korea Food and Nutrition Division, Department of Agrofood Resources, National Institute of Agricultural Sciences, Wanju, Cheonbuk, 55365, Korea School of Food Biotechnology and Nutrition, Kyungsung University, Busan, 48434, Korea
Investigating the survival response of Staphylococcus aureus to the antimicrobial lipid sphingosine

The human skin surface is covered with a lipid film that forms the interface between the viable epidermal layers and outer environment, and influences the colonisation of bacteria. Hydrolysis of the epidermal lipid ceramide via ceramidase yields sphingosine, an antimicrobial lipid that is able to kill staphylococci rapidly. Studies of patients with atopic dermatitis have indicated a correlation between levels of sphingosine and S. aureus colonisation of human skin, yet the contribution of sphingosine to the composition of the human skin microbiome and microbiota colonisation is unclear. This study investigates the effect of the host epidermal antimicrobial lipid D-sphingosine on S. aureus and S. epidermidis, with a focus on discovering components that contribute to survival of the former. The methods RNA-Seq and qPCR were used to dissect the transcriptional responses of S. aureus Newman and S. epidermidis Tu3298 after challenge with D-sphingosine. S. aureus displayed a large set of differentially expressed genes (1331), which contrasted with a smaller set for S. epidermidis (340). There was a clearly defined stimulon that was common between the species that included pathways for energy maintenance, amino acid and ion transport and metabolism, and protein repair. Previously reported transcriptomic responses to multiple cell wall and membrane-targeting antimicrobials shared commonalities with the response to sphingosine that supports its reported mode of action at the cell surface with membrane damage. The VraSR cell wall stress stimulon was upregulated in both species. Each species displayed pronounced upregulation of a putative phosphate-specific ABC transporter system encoded by the pstS-pstCAB operons. Experiments identified a contribution of this transport system to sphingosine survival and phosphate supplementation was shown to markedly increase sphingosine survival. Previous studies by the Horsburgh group identified experimentally evolved S. aureus strains with high levels of D-sphingosine resistance after serial passage with increasing concentrations of the lipid. SNPs associated with increased resistance were determined by genome resequencing and some of these were localised to the farER genes encoding a FarR-regulated FarE (mmpL) lipid efflux transporter. An isogenic farR SNP mutant of S. aureus was generated using molecular genetics approaches and showed a 10-fold higher MIC for D-sphingosine compared with the wild type Newman strain, indicating that the purported FarER fatty acid efflux system may have the capacity to mediate transport of sphingosine. The study findings of a link between sphingosine challenge and phosphate metabolism indicates future research potential to study host spingosine-1-phosphate in staphylococcal lifecycles, during health and disease.
Ultrafast Electrochemical Trigger Drug Delivery Mechanism for Nanographene Micromachines

Nano/micromachines with autonomous motion are the frontier of nanotechnology and nanomaterial research. These self‐propelled nano/micromachines convert chemical energy obtained from their surroundings to propulsion. They have shown great potential in diagnostic and therapeutic applications. This work introduces a high‐speed tubular electrically conductive micromachine based on reduced nanographene oxide (n‐rGO) as a platform for drug delivery and platinum (Pt) as the catalytic inner layer. n‐rGO/Pt micromachines are loaded with doxorubicin (DOX) by a simple physical adsorption with a very high loading efficiency, displaying single‐ or multistrand wrapping of DOX monomers on the micromachine cylinders. More importantly, it is found that electron injection into DOX@n‐rGO/Pt micromachines via electrochemistry leads to expulsion of DOX from micromachines in motion within only a few seconds. An in vitro study confirms this efficient release mechanism in the presence of cancerous cells. The unique properties of the n‐rGO/Pt micromotor enable the effective management of DOX release at the tumor site and thus enhances the therapeutic efficiency and reduces the side toxicity toward the healthy tissue. These micromachine drug carriers combine the high loading capacity of conventional carbon‐based drug carriers with a fast and efficient electrochemical drug‐release mechanism.
Caractérisation fonctionnelle de l'enzyme 17β-hydroxystéroïde déshydrogénase de type 12

The research project presented in this thesis was aimed at the functional characterization of type 12 17p-hydroxysteroid dehydrogenase (17P-HSD12), which catalyzes the conversion of estrone to estradiol, as opposed to its homolog 17P-HSD3, responsible for testicular production of testosterone. We first have observed that the adipocyte differentiation process is associated with an increase of 17P-HSD estrogenic activity, concomitant with elevated levels of 17P-HSD12 transcripts, thus suggesting an implication of this enzyme in the estrogen-producing function of human adipose cells. In addition, by the enzymatic characterization of monkey and rat 17p-HSD12 enzymes, we have been able to confirm the crucial role of amino acid in position 234 for the activity as well as the substrate specificity of this enzyme. These two enzymes show a bulky phenylalanine amino acid at this specific position, while mouse and C.elegans enzymes display smaller amino acids at position 234, thus enabling them to efficiently convert both androgens and estrogens. Finally, through the generation of a mouse model bearing a Hsdl7bl2 disrupted gene, we have been able to conclude that 17P-HSD12 possesses an additional physiological function, different from its estrogen-producing one. Indeed, despite the fact that we were expecting to observe an estrogen-deficient phénotype in this mouse, no homozygous null mouse could be observed, 17P-HSD12 deficiency leading to a complete in utero lethality, most probably due to the fatty acid elongation activity which has been previously attributed to enzyme 17P-HSD12. Altogether, these data support the idea that 17P-HSD12 is a bifunctional enzyme, exerting important actions in long chain fatty acids production as well as in active estrogens biosynthesis.