Miracle-dz/UniFAR-FTUs huggingface dataset:
The elimination effects of lavender essential oil on Listeria monocytogenes biofilms developed at different temperatures and the induction of VBNC state

Listeria monocytogenes is a typical foodborne pathogen that causes hard‐to‐treat bacterial infections, mainly due to its ability to form biofilm and enter into a viable but non‐culturable state (VBNC). In this study, we investigated the removal effects of four antimicrobial agents on L. monocytogenes biofilms formed at 32°C and 10°C, analysed the resistances of the mature biofilms to antimicrobial agents, and explored the VBNC state of cells in mature biofilms induced by lavender essential oil (LEO). The results showed that the growth of L. monocytogenes was completely inhibited when 1·6% (v/v) of the LEO was added. Meanwhile, the results of the crystal violet staining and XTT reduction method indicated that different concentrations of LEO significantly reduced L. monocytogenes biofilms biomass and metabolic activities, followed by sodium hypochlorite, lactic acid, and hydrogen peroxide. Moreover, the confocal laser scanning microscopy (CLSM) images confirmed that the treated biofilms became thinner, the structure was sparse, and the appearance was blurry. More interestingly, L. monocytogenes biofilms developed at 10°C were less susceptible to the sanitizers than those formed at 32°C. In addition, LEO presented a more significant dispersing effect on the biofilm cells, and 1/2 MIC to 4 MIC of LEO could induce fewer VBNC state cells in biofilm and plankton compared with sodium hypochlorite. This study indicated that the LEO could be considered as an ideal antibiofilm agent for controlling L. monocytogenes. But we should pay attention to the resistance of the biofilms developed at low temperatures.
PFAS Molecules: A Major Concern for the Human Health and the Environment

Per- and polyfluoroalkyl substances (PFAS) are a group of over 4700 heterogeneous compounds with amphipathic properties and exceptional stability to chemical and thermal degradation. The unique properties of PFAS compounds has been exploited for almost 60 years and has largely contributed to their wide applicability over a vast range of industrial, professional and non-professional uses. However, increasing evidence indicate that these compounds represent also a serious concern for both wildlife and human health as a result of their ubiquitous distribution, their extreme persistence and their bioaccumulative potential. In light of the adverse effects that have been already documented in biota and human populations or that might occur in absence of prompt interventions, the competent authorities in matter of health and environment protection, the industries as well as scientists are cooperating to identify the most appropriate regulatory measures, substitution plans and remediation technologies to mitigate PFAS impacts. In this review, starting from PFAS chemistry, uses and environmental fate, we summarize the current knowledge on PFAS occurrence in different environmental media and their effects on living organisms, with a particular emphasis on humans. Also, we describe present and provisional legislative measures in the European Union framework strategy to regulate PFAS manufacture, import and use as well as some of the most promising treatment technologies designed to remediate PFAS contamination in different environmental compartments.
User Experience Design Elements and Application in Product Design

Starting from the user experience,the objectives of the user experience and the connotation of user experience design are analyzed.Based on the previous one,the six elements of user experience design are proposed.They are products functional demand,products sensory demand,products interactive demand,products emotional demand,products social demand and products self demand.And taking mobile phone as example,the application in products design from the six elements is discussed in more detail.Point out that the user experience design coming from people's lifestyle and interest,and creating a sensory experience and the psychological identification has become a new design method.
Humans sense by touch the location of objects that roll in handheld containers

Humans use active touch to gain behaviourally relevant information from their environment, including information about contained objects. Although most common, the perceptual basis of interacting with containers remains largely unexplored. The first aim of this study was to determine how accurately people can sense, by touch only, the location of a contained rolling object. Experiment 1 used tubes containing physical balls and demonstrated a considerable degree of accuracy in estimating the rolled distance. The second aim was to identify the relative effectiveness of the various available physical cues. Experiment 2 employed virtual reality technology to present, in isolation and in various combinations, the constituent haptic cues produced by a rolling ball, which are, the mechanical noise during rolling, the jolts from an impact with an internal wall, and the intensity and timing of the jolts resulting from elastic bounces. The rolling noise was of primary importance to the perceptual estimation task suggesting that the implementation of the laws of motion is based on an analysis of the ball’s movement velocity. Although estimates became more accurate when the rolling and impact cues were combined, they were not necessarily more precise. The presence of elastic bounces did not affect performance.
Moxibustion for anorexia in COVID-19

Abstract Background: As the outbreak of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread over the world, the World Health Organization has declared the outbreak of COVID-19 an international public health emergency. Besides typical respiratory symptoms and signs of COVID-19, digestive symptoms and liver injury have been frequently reported during the course of the disease. The purpose of this study was to evaluate the efficacy and safety of moxibustion in the treatment of anorexia in patients with COVID-19. Methods: According to the retrieval strategies, randomized controlled trials on moxibustion therapies for C19-A will be obtained from the China National Knowledge Infrastructure, WanFang Data, Chinese Scientific Journals Database, PubMed, Embase, and Cochrane Library, regardless of publication date or language. Studies will be screened based on inclusion and exclusion criteria, and the Cochrane risk bias assessment tool will be used to evaluate the quality of the literature. The network meta-analysis will be performed with the Markov chain Monte Carlo method and carried out with Stata 14.2 and WinBUGS 1.4. 3 software. Ultimately, the quality of the evidence obtained from the results will be evaluated. Results: This study will evaluate whether moxibustion therapy can effectively treat anorexia in patients with COVID-19. Conclusion: This study will provide evidence for whether moxibustion therapy is beneficial to the treatment of anorexia in patients with COVID-19. Trial registration number: CRD42022302499
Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor

Polycyclic aromatic hydrocarbons (PAHs) and their N- and O-containing derivatives (N-/O-PAHs) are environmental pollutants and synthetically attractive building blocks in pharmaceuticals. Functionalization of PAHs can be achieved via C-H activation by cytochrome P450 enzymes (e.g., P450 CYP3A4) in an environmentally friendly manner. Despite its broad substrate scope, the contribution of CYP3A4 to metabolize common PAHs in humans was found to be small. We recently showcased the potential of CYP3A4 in whole-cell biocatalysis with recombinant yeast Komagataella phaffii (Pichia pastoris) catalysts for the preparative-scale synthesis of naturally occurring metabolites in humans. In this study, we aimed at exploring the substrate scope of CYP3A4 towards (N-/O)-PAHs and conducted a bioconversion experiment at 10 L scale to validate the synthetic potential of CYP3A4 for the preparative-scale production of functionalized PAH metabolites. Hydroxylated products were purified and characterized using HPLC and NMR analysis. In total, 237 mg of fluorenol and 48 mg of fluorenone were produced from 498 mg of fluorene, with peak productivities of 27.7 μmol/L/h for fluorenol and 5.9 μmol/L/h for fluorenone; the latter confirmed that CYP3A4 is an excellent whole-cell biocatalyst for producing authentic human metabolites.
Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis

Background There is a need to support the diagnosis of anaphylaxis by objective markers. miRNAs are promising noncoding RNA species that may serve as serological biomarkers, but their use in diagnosing anaphylaxis has not been systematically studied to our knowledge. We aimed to comprehensively investigate serum biomarker profiles (proteins, lipids, and miRNAs) to support the diagnosis of anaphylaxis. Methods Adult patients admitted to the emergency room with a diagnosis of anaphylaxis (<3 hours) were included. Blood samples were taken upon emergency room arrival and 1 month later. Results Next-generation sequencing of 18 samples (6 patients with anaphylaxis in both acute and nonacute condition, for 12 total samples, and 6 healthy controls) identified hsa-miR-451a to be elevated during anaphylaxis, which was verified by quantitative real-time PCR in the remaining cohort. The random forest classifier enabled us to classify anaphylaxis with high accuracy using a composite model. We identified tryptase, 9α,11β-PGF2, apolipoprotein A1, and hsa-miR-451a as serological biomarkers of anaphylaxis. These predictors qualified as serological biomarkers individually but performed better in combination. Conclusion Unexpectedly, hsa-miR-451a was identified as the most relevant biomarker in our data set. We were also able to distinguish between patients with a history of anaphylaxis and healthy individuals with higher accuracy than any other available model. Future studies will need to verify miRNA biomarker utility in real-life clinical settings. Funding This work is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) as part of the clinical research unit (CRU339): Food Allergy and Tolerance (FOOD@) (project number 409525714) and a grant to MW (Wo541-16-2, project number 264921598), as well as by FOOD@ project numbers 428094283 and 428447634.
A Structural View of miRNA Biogenesis and Function

Micro-RNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that act as post-transcriptional regulators of gene expression. Since their discovery in 1993, they have been the subject of deep study due to their involvement in many important biological processes. Compared with other ncRNAs, miRNAs are generated from devoted transcriptional units which are processed by a specific set of endonucleases. The contribution of structural biology methods for understanding miRNA biogenesis and function has been essential for the dissection of their roles in cell biology and human disease. In this review, we summarize the application of structural biology for the characterization of the molecular players involved in miRNA biogenesis (processors and effectors), starting from the X-ray crystallography methods to the more recent cryo-electron microscopy protocols.
Data excellence for AI

This forum provides a space to engage with the challenges of designing for intelligent algorithmic experiences. We invite articles that tackle the tensions between research and practice when integrating AI and UX design. We welcome interdisciplinary debate, artful critique, forward-looking research, case studies of AI in practice, and speculative design explorations. --- Juho Kim and Henriette Cramer, Editors
Container-aware I/O stack: bridging the gap between container storage drivers and solid state devices

Solid State Devices (SSDs) have been widely adopted in containerized cloud platforms as they provide parallel and high-speed data accesses for critical data-intensive applications. Unfortunately, the I/O stack of the physical host overlooks the layered and independent nature of containers, thus I/O operations require expensive file redirect (between the storage driver, Overlay2/EXT4, and the virtual file system, VFS) and are scheduled sequentially. Moreover, containers suffer from significant I/O contention as resources at the native file system are shared between them. This paper presents a Container-aware I/O stack (CAST). CAST is made up of Layer-aware VFS (LaVFS) and Container-aware Native File System (CaFS). LaVFS locates files based on layer information and enables simultaneous Copy-on-Write (CoW) operations and thus avoids the overhead of searching and modifying files. CaFS, on the other hand, provides contention-free access by designing fine-grain resource allocation at the native file system. Experimental results using a NVMe SSD with micro-benchmarks and real-world applications show that CAST achieves 216%-219% (38%-98%, respectively) improvement over the original I/O stack.
Impact of Donepezil on Brain Glucose Metabolism Assessed Using [18F]2-Fluoro-2-deoxy-D-Glucose Positron Emission Tomography Imaging in a Mouse Model of Alzheimer’s Disease Induced by Intracerebroventricular Injection of Amyloid-Beta Peptide

Translational methods are needed to monitor the impact of the Alzheimer’s disease (AD) and therapies on brain function in animal models and patients. The formation of amyloid plaques was investigated using [18F]florbetapir autoradiography in a mouse model of AD consisting in unilateral intracerebroventricular (i.c.v) injection of amyloid peptide Aβ25–35. Then, an optimized positron emission tomography (PET) imaging protocol using [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) was performed to estimate brain glucose metabolism: [18F]FDG was injected in awake animals to allow for 40 min brain uptake in freely moving mice. Anesthesia was then induced for 30 min PET acquisition to capture the slow and poorly reversible brain uptake of [18F]FDG. Impact of donepezil (0.25 mg/kg daily, 7 days, orally) on brain function was investigated in AD mice (n = 6 mice/group). Formation of amyloid plaques could not be detected using autoradiography. Compared with sham controls (injection of scramble peptide), significant decrease in [18F]FDG uptake was observed in the AD group in the subcortical volume of the ipsilateral hemisphere. Donepezil restored normal glucose metabolism by selectively increasing glucose metabolism in the affected subcortical volume but not in other brain regions. In mice, [18F]FDG PET imaging can be optimized to monitor impaired brain function associated with i.c. v injection of Aβ25–35, even in the absence of detectable amyloid plaque. This model recapitulates the regional decrease in [18F]FDG uptake observed in AD patients. [18F]FDG PET imaging can be straightforwardly transferred to AD patients and may aid the development of certain therapies designed to restore the altered brain function in AD. Graphical Abstract [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) PET imaging was performed in a mouse model of Alzheimer’s disease to investigate the impact of donepezil on brain glucose metabolism.
The Influence of Flexible Employment on Workers’ Wellbeing: Evidence From Chinese General Social Survey

Based on the 2017 China General Social Survey data, with 5,439 observations as research objects, this paper empirically tests the impact of flexible employment on workers’ wellbeing and introduces labor income as mediator and social security as moderator to explore the mechanism of action. The empirical results show that: flexible employment has an inverted U-shaped relationship with workers’ wellbeing, which indicates that increasing employments’ flexibility will first rise and then reduce their perceived subjective wellbeing after reaching the peak; labor income plays a mediating role in the relationship of flexible employment and wellbeing of workers; social security moderates the mediating effect of labor income whereas the moderating role in the relationship between flexible employment and workers’ wellbeing is not observed. Implications and future development of flexible employment are discussed.
Investigation and management of stillbirth: a descriptive review of major guidelines

Abstract Stillbirth is a common and devastating pregnancy complication. The aim of this study was to review and compare the recommendations of the most recently published guidelines on the investigation and management of this adverse outcome. A descriptive review of guidelines from the American College of Obstetricians and Gynecologists (ACOG), the Royal College of Obstetricians and Gynecologists (RCOG), the Perinatal Society of Australia and New Zealand (PSANZ), the Society of Obstetricians and Gynecologists of Canada (SOGC) on stillbirth was carried out. Regarding investigation, there is consensus that medical history and postmortem examination are crucial and that determining the etiology may improve care in a subsequent pregnancy. All guidelines recommend histopathological examination of the placenta, genetic analysis and microbiology of fetal and placental tissues, offering less invasive techniques when autopsy is declined and a Kleihauer test to detect large feto–maternal hemorrhage, whereas they discourage routine screening for inherited thrombophilias. RCOG and SOGC also recommend a complete blood count, coagulopathies’ testing, anti-Ro and anti-La antibodies’ measurement in cases of hydrops and parental karyotyping. Discrepancies exist among the reviewed guidelines on the definition of stillbirth and the usefulness of thyroid function tests and maternal viral screening. Moreover, only ACOG and RCOG discuss the management of stillbirth. They agree that, in the absence of coagulopathies, expectant management should be considered and encourage vaginal birth, but they suggest different labor induction protocols and different management in subsequent pregnancies. It is important to develop consistent international practice protocols, in order to allow effective determination of the underlying causes and optimal management of stillbirths, while identifying the gaps in the current literature may highlight the need for future research.
Characterisation and Modelling Lithium Titanate Oxide Battery Cell by Equivalent Circuit Modelling Technique

Lithium Titanate Oxide (L TO) battery cells have immense potential as energy storage systems in large-scale stationary grid applications due to their better cycling performance, lower self-discharge and higher safety margins compared to other Lithium based battery chemistries. Hence, accurate L TO performance models at various operating conditions are required for different purposes like determination of their dynamic behaviour, modelling LTO's suitability to an application as well as to the development of battery and energy management systems (BMS and EMS). L TO battery cell performance is mainly affected by parameters such as, state of charge (SOC) and temperature. Hence, in this paper, second order equivalent circuit model (ECM) of an L TO cell will be developed based on their experimental characterisation at different SoC's (every 10% intervals) and temperatures (15 °C, 25 °C, 35 °C and 45 °C), Modified version of the Hybrid pulse power characterisation (HPPC) test method will be utilised for parametrisation of the ECM of 2.9 Ah L TO cell. The simulation model will be developed in Matlab/Simulink platform and compared with the laboratory measurements for ECM validation.
Development of a Wearable Scoliosis Monitoring System Using Inertial Sensors

Monitoring human motion with magnetic and inertial measurement units is a complex task and there are many factors that must be taken into consideration. In this work, a wearable system for monitoring scoliosis using three inertial measurement units (IMUs) is introduced. The proposed solution can be used indoor and is focused on using the roll angle for measuring lateral movement of the spine, which characterizes the scoliosis spinal disorder.
Design of Daily Safety Management System for Metro Traffic Engineering

Different from the existing project management system, the daily safety management system of rail transit projects has high public safety requirements, and has the characteristics of high cost, high risk, and technical complexity. Once a major safety accident occurs, the social impact will be large and the loss will be huge. Based on the data obtained in the safety inspection, advanced digital information methods to increase the safety management of subway construction are derived from the results of several safety inspections in various aspects in each cycle. This article discusses the design of a safety management system based on daily subway operations.
Research on Fault Diagnosis Method of Power Transformer Based on Graph Neural Network

Artificial neural networks are often used on transformer fault diagnosis. It takes the independent characteristic gas content or gas ratio as the input parameter, lacks the consideration of the correlation between gases. To solve this problem, a transformer fault diagnosis method based on graph neural network(GNN) is proposed in this paper. Relationship between gases is described by a directed graph. For the advantages on dealing with unstructured data, A GNN is designed in this paper to extract correlations characteristic between gases. The relationship between the gas characteristics and the fault type is established by a multi-layer linear neural network, then, the fault type is finally determined. The experimental results show that, compared with the methods using convolutional neural network(CNN), multilayer perceptron(MLP), K-nearest neighbor(KNN) and support vector machine(SVM), the method proposed in this paper achieves deep extraction of gas characteristics and improves diagnosis accuracy for each fault type of power transformers.
Investigation on Distributed Coordinated Control Method of DFIGs Participating in Power System Frequency Regulation

A distributed coordinated frequency control method is proposed for the power system with doubly fed wind turbines (DFIGs) and conventional generators, which can improve the frequency response characteristics of the system. The DFIG operation mode is selected according to the relationship between supply and demand. If the load demand is greater than DFIG maximum output power, the deloading level of DFIG is determined by the proposed distributed control method to quickly respond to the system frequency changes. The proposed distributed control method can coordinate DFIG under different wind speed conditions, and achieve coordinated control with thermal power units according to the available power of DFIG and system frequency deviation, so as to effectively improve the dynamic performance of the system. Finally, simulation analysis is carried out to verify the effectiveness of the proposed distributed coordinated frequency control method.