Miracle-dz/UniFAR-FTUs huggingface dataset:
Nonviral mcDNA-Mediated Cospecific CAR T Cells For The Treatment of Human Hepatocellular Carcinoma Xenograft in Mice

Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and the adoptive immunotherapy of which is worth studying. CD133, a kind of cancer stem cell (CSC) antigen, together with glypican-3 (GPC3) have been proved to be highly expressed in HCC cells and both of them are used as targets to generate chimeric antigen receptor (CAR) T cells. But there are limitations like “on-target, off-tumor” toxicity, low transfection efficacy and weak antitumor ability in CAR T cells treatment.Methods: First we fused anti-CD133 and anti-GPC3 single chain Fragment variable (scFv) structures with intracellular domains, respectively. Using non-viral minicircle DNA (mcDNA) vectors to generate co-specific CAR T cells (CoG133-CAR T cells) against CD133 and GPC3 double-positive HCC cells. We exhibited the transduction efficiency of CoG133-CAR T cells and the antigen expression of tumor cell lines. Finally, the antitumor efficacy of CoG133-CAR T cells both in vitro and in vivo was detected. Results: GPC3-CAR and CD133-CAR were successfully prepared using non-viral mcDNA vectors to generate effector cells. For the GPC3 and CD133 double-positive HCC (Huh7) xenograft mice, co-specific CAR T cells possessed stronger tumor growth suppression compared to single-targeted CAR (GPC3-CAR and CD133-CAR) T cells which induced only one antigen-mediated signal pathway. The same results also occurred on the in vitro experiments including cytokine secretion, cytotoxicity and proliferation ability of CAR T cells. Vital organs from CoG133-CAR T cells and normal T cells respectively treated Huh7 xenograft mice were stained by hematoxylin and eosin (H&E), the images showed no difference. Conclusions: The mcDNA vectors loading CAR structures were transfected into T cells by electroporation without genetic mutation or mismatch. Huh7 is an HCC cell line with two antigens of GPC3 and CD133 highly expressed. The antitumor efficacy of co-specific CAR (CoG133-CAR) T cells against Huh7 cells is significantly enhanced. The joint design of two specific targets and non-viral vectors leads much more safety, also.
Development of Framework for Rapid Tool Manufacture for RIDFT Process

Polymer composites are in a period of significant growth due to the increased use in the automobile, marine and aerospace application. Some of the advantages of using composites over other materials are weight savings, corrosion resistance and functional integration. However, long cycle times and higher tooling costs of the available manufacturing processes make it difficult to mass-produce composite products. Resin infusion double flexible tooling (RIDFT) is a novel process developed by the Florida Advanced Center for Composite Technologies (FACT) at FAMU-FSU College of Engineering aimed at tackling some of the above-mentioned problems. The use of onesided mold provides a huge cost advantage over Resin Transfer Molding (RTM). However, using cost effective materials can reduce the cost further. In this thesis research, the various steps involved in the manufacture of the RIDFT mold were identified. The seven steps to manufacture a RIDFT mold are, Initial Graphics Exchange Standard (IGES) file tooling import/repair, mold design, material selection and preparation, NC programming, machine setup, machining and finishing and polishing. The problems at each of these stages were identified. The solutions to some of the problems, which might result in decrease in the tool manufacturing time, were found. To tackle with IGES file import problems some guidelines are presented. The various steps involved in the mold design stage are: compensation of permeable layer thickness, compensation of silicone layer thickness compensation of thickness of part and addition of draft surface. The tooling parameters compensation part of the mold design process was implemented by using both offset and scaling methods using C programming. The materials used for tooling until now are reviewed for pros and cons. Some of the tooling materials, that can be used in future, are also suggested. In order to help the mold
Setting Reward Function of Sensor Based DDQN Model

In this study, it is aimed to determine the appropriate reward function of the agent which trained to pass 100 obstacles/objects in Reinforcement Learning (RL) with Double Deep Q Network (DDQN) model. To train the agent, environment is split into sub problems. Several rules and different reward functions defined for the sub problems. A developed mini deep learning library which is called gNet is used for the training.
Deep Reinforcement Learning-Aided Efficiency Optimized Dual Active Bridge Converter for the Distributed Generation System

With the development of power system electronization, distributed new energy generation-based DC microgrid system has become a hot spot of the current research. Aiming to implement the collection, transformation and transmission of the new energy sources, the dual active bridge (DAB) converter has become a key energy conversion device. Aiming to promote the transmitted efficiency for such DAB converter, a deep reinforcement learning (DRL) optimization scheme with the triple-phase-shift (TPS) is proposed. Specifically, the deep deterministic policy gradient (DDPG) algorithm is used for solving optimal solutions with the minimum power losses. Then, the trained DDPG agent is used as a fast surrogate predictor, which giving the suitable control decisions under whole operation conditions. The main control objective is to minimize the power losses for getting the target maximum efficiency. Besides, the soft switching constrains are added to the DDPG algorithm. Based on this, the proposed optimized method can be used for different operation conditions with excellent performance. The operation principles, the loss analyses, and the training consideration and evaluation of the DDPG algorithm are given. Finally, simulation and experimental results using a 200 W hardware prototype are displayed and compared to prove to effectiveness of theoretical analysis.
A STUDY ON THE EFFECTS OF THE ACTIVE YAW MOMENT CONTROL

This paper presents a new torque distribution system, called the "Right/Left Torque Control System". The aim is to improve a vehicle's cornering properties by using yaw moment control. The torque transfer mechanisms of this system have been analyzed. A yaw moment control algorithm using yaw rate feedback control has been designed. Vehicle cornering properties were evaluated using numerical simulation developed from data taken from an actual vehicle. As a result, improvements were achieved in the manoeuverability and stability of a vehicle during cornering. (A) For the covering abstract of the conference see IRRD 875861.
External Debt, Public Investment and Economic Growth in Cameroon

This study investigates the extent to which external debt and public investment contributes to economic growth in Cameroon - emphasising on how public investment modulates the effect of external debt on economic growth. Time series data spanning the period 1980-2018 obtained from the World Bank’s world development indicators are used, together with the Dynamic Ordinary Least Squares (OLS) approach to ascertain the nature of the long-run relationship between external debt, public investment and economic growth. Consistent with the debt-overhang and crowding-out literature, the study reveals a negative significant influence of external debt on economic growth in Cameroon. Results also reveal that there is a positive and significant direct effect of public investment on economic growth in the long run. Further results indicate that public investment and external debt positively and significantly engender economic growth. This is evidence that public investment is modulating the effect of external debt on economic growth in Cameroon. These findings suggest the need for developing country governments to create an enabling environment for private sector development, while accompanying external debt resources with domestic revenue mobilization by broadening the tax base - taxes on landed property being potential candidates
A meandering substrate integrated waveguide with slots for frequency scanning array

A meandered substrate integrated waveguide (SIW) has been proposed for the frequency scanning array that has potential application of the low cost 24‐GHz Doppler radar system. All radiating slots are connected in series using a short circuit ended SIW; therefore, its radiation beam can be steered with frequency. The modified antenna with meandering structure is proposed to enhance the scanning range. The physical slot spacing is kept at about half free space waveguide, whereas the electrical spacing is about 540° at the center frequency of 24 GHz. A broadside radiation is achieved at 24‐GHz frequency and a ±30° continuous scanning range is experimentally confirmed with the frequency band from 23 to 25 GHz. Another antenna with center frequency of 77 GHz is designed and tested. It also achieves a ±30° continuous scanning range with the frequency band from 75 to 79 GHz. © 2014 Wiley Periodicals, Inc. Microwave Opt Technol Lett 56:1478–1483, 2014
A comparative study of tendinous interconnection in the forearm and hand of human cadavers and live subjects

Certain complex hand functions such as playing musical instruments, buttoning and writing require the action of the long flexor tendons. Repeated movements of these tendons cause inflammatory changes resulting in an increase in the Tendon Cross Sectional Area (TCSA), and, subsequently, tendinous interconnections (occurs in approximately 20% of the general population, commonly between the flexor pollicis longus [FPL] and index finger flexor digitorum profundus [FDP] - Linburg-Comstock syndrome). Coupled with an anatomically congested space at the wrist, such interconnections may compress the median nerve resulting in carpal tunnel syndrome. This study evaluated the prevalence of tendinous interconnection amongst 200 medical students at Cardiff University by a series of structured hand movements (n=12) in controlled setting. The findings were corroborated using Ultrasound Scan (USS) (n=4) and Magnetic Resonance Imaging (MRI) (n=1). The muscle mass, fibre length, density, angle of pennation and tendon length were ascertained by studying hands and forearms (n=30) of embalmed human cadavers. The data was used to determine the Physiological Cross Sectional Area (PCSA) and calculate the relation between PCSA and TCSA. Simple linear regression established the direct relation of PCSA to TCSA, muscle mass and tendon length. A direct relation between the TCSA of FPL and index finger FDP was identified. The cross sectional area of the median nerve increases as it traverses the carpal tunnel. The PCSA had no bearing on density and angle of pennation. USS was found to be more dynamic and sensitive than MRI in identifying tendinous and tenosynovial interconnections, as the tenosynovial interconnections <1 mm thick could be recognised. The study also (i) identified a lower percentage of tendinous interconnection (compared to the literature) in a randomly selected group of individuals, (ii) detected the limitation of photograpic measurements to study angles of finger movements and (iii) highlighted the disadvantage of calculating PCSA in cadavers.
Evaluation of Gastroprotective Activities of Fraction Extracts of Piper guineense Leaf on Ethanol-Induced Ulcer in Wistar Rats

One of the approaches towards alleviation of peptic ulcer disease is by use of antiulcer agents. Cost factor has an important role to play in the choice of antiulcer agents in ulcer therapy. Piper guineense, a flora to tropical Africa, is claimed by traditional medicine as a remedy for peptic ulcer. Despite its wide usage in crude form, purified form of Piper guineense are yet to be explored. The aim of the study was to evaluate the gastroprotective activities of fraction extracts of Piper guineense on ethanol-induced ulcer in wistar rats. This was done by administering orally, 400mg/kg of various fraction extracts to six test groups, 100mg/kg cimetidine to a positive control group and 5ml/kg Tween 80 (3% v/v) to a negative control group. Histological study and effect of the extracts on stomach weight were conducted. Findings revealed that extracts significantly (p<0.05) inhibited gastric ulceration with PF-4 producing 49.02% ulcer inhibition while cimetidine produced 81.93%. Histological study revealed gastroprotection with minor epithelial loss in extracttreated group. The extracts (PF-2, PF-4. EE) respectively produced 23.47%, 27.23% and 33.33% significant (p<0.05) increase in stomach weight of rats. In conclusion, the fraction extracts of Piper guineense possess gastroprotective activities.
COOPERATIVE COMMUNICATIONS AND NETWORKING: CASE STUDY ON CROSS-LAYER DESIGN AND OPTIMIZATION OF COGNITIVE RADIO NETWORKS

: Today wireless technologies simply coexist without any coordination (within one technology as well as among different technologies). This leads to more and more unwanted and uncontrolled interference, and hence inefficient spectrum usage, which is getting more problematic as the density of wireless devices increases. Radio spectrum is the most valuable resource in wireless communication. The cognitive radio and cognitive based networking are transforming the static spectrum allocation based communication systems in to dynamic spectrum allocation. Cognitive radios are intelligent devices with ability to sense environmental conditions and can change its parameters according to the requirements to get the optimized performance at the individual nodes or at network level. This paper covers the basics and origin of software defined radio, cognitive radio, cognitive radio network, cognitive cycle, signalling, Cognitive Radio Sensor Networks and the concept of cross layer design. The performance metrics explain the node and network level performance measurements. This paper also covers the different network paradigms.
The Role of Women Headed Households in the Local Seed Business in Ethiopia: The Case of Jabi Tehnan, Bure and Yilmana Densa Districts

This study was conducted to assess the role of women headed households in the Local Seed Businesses and to analyze the determinant factors that influence women headed households benefit in the local seed business. The research method of this study was quantitative research method and descriptive research design was employed as a research design. Women in the local seed business were most frequently participated in seed production activities such as weeding, harvesting and storage of seeds, chemical fertilizer application and sowing of seeds. On the other hand, their participation in leadership, applying pesticides, trainings, organic fertilizer use, and active participation in meetings were limited. The Binary Logistic Regression analysis indicated different variables that determine women headed households benefit status in seed producer cooperatives, that factors such as land plot size, land used for local seed business, soil fertility, oxen ownership, access to market, access to credit services and access to extension services had significant effects on benefits gained by women headed households from the local seed business. To enhance the involvement of women headed households in the local seed business and to enable them benefit from their efforts it seems imperative that government and non government institutions should develop a solid package on seed production and carry out capacity buildings that could empower women.
Selection of Effective Clouds for Data Hosting Services

In the current trend companies are moving their computing infrastructure to cloud computing from the traditional servers. The cloud hosting provides more features compared to the traditional hosting. Ability to grow at faster rate, handle huge load at the time of surge traffic. Enterprises do not need to buy/invest in hardware. We lease the infrastructure in cloud. Cloud will take away burden of server maintenance. Cloud give us flexibility to pay as per usage not a fixed cost per machine/server, we do not worry about idle servers. Organizations do not worry about the throughput or capacity of the servers as cloud provides the high availability and ability to grow and shrink as per the demand, this feature makes systems to handle huge traffic at short durations. Cloud provides replication across different servers which are spread across multiple geographical locations in the world. Cloud data centers are located in multiple locations makes us to host the server near to the location of users. Clouds are expensive compared to the traditional services. They are not flexible in terms of costs. In this paper, we explain the concept of Selection of Effective Clouds for Data Hosting Services, which provides cheaper data hosting using multiple cloud services which ensures high availability of data. It provides two key features which makes it better than the single cloud hosting. The first is using more than one cloud service provides high availability, even if one cloud services are down, still the file will available. The second is replication algorithm to store data in multiple clouds and retrieve the data from multiple clouds without any data loss. The distribution load is based on the pricing of clouds. This system makes sure we get optimum price. On an evaluation of the performance of our system, results show that it is able to model accurately the performance of cloud and to leverage this for efficient data dissemination, being able to reduce the monetary costs and transfer time. Keywords—Cloud computing, Multi-Cloud, Data Hosting, Cost Efficient, Cloud Storage.
A comparison of the Krasnoselskii spectrum and the homotopy significant spectrum

Instead of deﬁning eigenvalues only for linear maps, it is possible to deﬁne them for arbitrary continuous functions. First we however discuss in detail a proof of the Borsuk-Ulam theorem. We use the result to prove for quadratic forms that the set of eigenvalues found by the Courant-Fisher min-max principle equals the Krasnoselskii spectrum. Then we move on to the homotopy signiﬁcant spectrum as deﬁned by Gromov for functions deﬁned on the real projective space. We prove that the Krasnoselskii spectrum for these functions is contained in the homotopy signiﬁcant spectrum. In the case of the real projective line and plane we prove that the Krasnoselskii spectrum equals the homotopy signiﬁcant spectrum. For the three-dimensional real projective space we prove that the Krasnoselskii spectrum forms a proper subset of the homotopy signiﬁcant spectrum.
Design of a Detachable Bronchoscope

Cauwenberghs 1 Stephen Deiss 2 Gopabandhu Hota Di Ventra Yuanhang Zhang Fullerton 1 Yuxuan Xiao 2 Jeffrey Brock 3 Biswajit Sahoo 4 Ruben Saatjian Kummel Scott Ueda Lomakin 1 Fangzhou Ai 2 Zhuonan Lin 3 Iana Volvach 4 Xueyang Wang Nomura Chi-Hsin Huang Schuller 1 Ben Erbin Qiu 2 Pavel Salev 3 Henry Navarro Siegel 1 Dongmin Shin 2 Karthik Nagarjuna 3 Omer Sabary 4 Simeng Zheng Talke 1 Matthew Kohanfars 2 Rafaela Simoes-torigoe 3 Bryan Nguyen 4 Alex Phan
The Term Structure of Expectations

Economic theory predicts that intertemporal decisions depend critically on expectations about future outcomes. Using the universe of professional survey forecasts for the United States, we document the behavior of the entire term structure of expectations for output growth, inflation, and the policy rate. We show that a simple unobserved components model of the trend and cycle explains the joint behavior of both consensus measures of expectations and the observed disagreement among individual forecasters. Importantly, univariate models of each variable are outperformed by a multivariate model of the joint dynamics of these three variables, particularly for nominal interest rates. Consistent with the data, the model predicts a link between revisions in long-run expectations to short-term forecast errors. In structural models, learning about the long run has important empirical and theoretical implications for monetary and fiscal policy.
Variation in French Accentual Phrase Realization. The case of penultimate marking

This paper aims at determining whether the presence of penultimate accentuation varies across the varieties of French, at examining acoustic correlates of penultimate prominence marking in a corpus-based perspective, and at discussing the status of such a phenomenon in French phonology. In French, the minimal prosodic unit for pitch accent assignment is not the Phonological Word, but a higher constituent in the prosodic hierarchy, the Accentual Phrase (AP). In the varieties of French spoken in the North of France, AP is tonally marked by a high pitch movement associated with its rightmost full syllable and an optional high tone associated with a syllable on its left (see the LHiLH* pattern identified by Jun & Fougeron [2002]). In the regional varieties of French spoken in the periphery of the Hexagon (Belgium and Switzerland); one variant of this pattern involves the realization of a prominence on the penultimate syllable of the AP. Little work has been dealing with such a prosodic pattern. Yet, the phonological status and the phonetic features associated with penultimate prominent marking in French still remain unsolved issues. In order to examine this question, we analyzed data extracted from the "Phonologie du Francais Contemporain" (PFC) database [Durand et al. 2009]. In all, 6 varieties were selected: one variety from France (Paris, Lyon), three varieties spoken in the French-speaking part of Switzerland (Geneva, Neuchâtel, Nyon and Martigny) and two varieties from the French-speaking part of Belgium (Tournai and Liege). For each of these 8 varieties, we selected the recordings of the same text read by 8 speakers. In order to examine the presence of a prominent penultimate syllable within the AP, we first identified the APs boundaries. Then, we coded the penultimate syllable of each AP as prominent or non-prominent. On the other hand, to examine the acoustic features involved in the realization of a penultimate syllable perceived as prominent, we computed, for each parameter (duration, F0, amplitude and F0 rise), the number of UPs ("Unite de perception", according to Rossi [1979]). Taken together the results show that, despite the fact that penultimate prominences appear in the 6 varieties, not all acoustic cues are involved in the same in their realization in the different varieties, and that the question of the phonological status of penultimate marking in French is a complex problem