Miracle-dz/UniFAR-FTUs huggingface dataset:
T-Cell Receptor Repertoire of Lymphocytes Infiltrating Cutaneous Melanoma Is Predominated by Va Specificities Present in T-Cells of Normal Human Skin1

Lymphocytes infiltration solid tumors can be propagated in vitro with interleukin 2 and are then capable, as (.'1)8+ cytotoxic T-cells, of specific lysis of autologous tumor targets in a class I-restricted manner. Since the specificity of these cells is determined by their T-cell receptor (TCR) configuration, the aim of this study was to delineate the TCR Va reper toire of tumor-infiltrating lymphocytes within 24 melanoma specimens and to compare these data with the TCR expression pattern of unaffected peritumoral and normal human skin. While lymphocytes within all skin specimens tested expressed a substantial, albeit limited, heterogeneity of Va specificities with an average number of 9.0 different Va gene seg ments, the Va repertoire within cutaneous melanoma lesions was signifi cantly more restricted (mean of Va expression, 3.86; P < 0.001) with a predominance of only 3 Va families ( Val3, Vit 15, and Va7e ), all of which were also found to be expressed within normal skin. Collectively, the fact that the TCR Va repertoire in melanoma is skewed toward the predom inance of only a few Va regions may be indicative of a limited number of melanoma-associated antigenic determinants being involved in the antitumor immune response.
OTT_A_259616 7941..7948

1Department of Geriatric Oncology, Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing, People’s Republic of China; 2Department of Neurosurgery, Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing, People’s Republic of China Abstract: Generations of epidermal growth factor receptor tyrosine kinase inhibitors (EGFRTKIs) can significantly improve the outcome of EGFR-positive NSCLC patients. However, acquired TKIs-resistant mutations are inevitable. Except the common EGFR alterations, more and more rare mutations are revealed by next-generation sequencing (NGS), the clinical significance of which are still unclear. Here, we report an advanced lung adenocarcinoma patient who harbored two novel EGFR exon 19 deletions (750_758del and I759S) at the beginning and exhibited a short response to icotinib for 7.0 months. Then, secondary resistance EGFR T751_I759delinsS occurred. Chemotherapy combined with bevacizumab and erlotinib was administered in turn but failed. Standard-dose osimertinib (80 mg daily) obtained durable clinical remission for 16 months, and high-dose osimertinib (160 mg daily) further prolonged the survival of 9 months after leptomeningeal metastases (LM) occurring. This study presented the first case of intractable terminal NSCLC in a patient with EGFR 750_758del, I759S and T751_I759delinsS mutations, who responded positively to osimertinib and achieved a prolonged OS of 52 months, providing a potential therapeutic option for the patients harboring these particular EGFR mutations.
Prospective, open-label, randomized, phase III study of two dose-dense regimens MVAC versus gemcitabine/ cisplatin in patients with inoperable, metastatic or relapsed urothelial cancer: a Hellenic Cooperative Oncology Group study

Prospective, open-label, randomized, phase III study of two dose-dense regimens MVAC versus gemcitabine/ cisplatin in patients with inoperable, metastatic or relapsed urothelial cancer: a Hellenic Cooperative Oncology Group study (HE 16/03) A. Bamias1, U. Dafni2, A. Karadimou1, E. Timotheadou3, G. Aravantinos4, A. Psyrri5, I. Xanthakis2, M. Tsiatas1, V. Koutoulidis6, C. Constantinidis7, C. Hatzimouratidis8, E. Samantas4, A. Visvikis4, M. Chrisophos9, K. Stravodimos7, C. Deliveliotis9, A. Eleftheraki10, D. Pectasides11, G. Fountzilas3 & M. A. Dimopoulos1 Department of Clinical Therapeutics, Alexandra Hospital, Athens University Medical School, Athens; Laboratory of Biostatistics, University of Athens School of Nursing, Athens; Department of Medical Oncology, Papageorgiou Hospital, Aristotle University of Thessaloniki School of Medicine, Thessaloniki; 3rd Department of Medical Oncology, Agii Anargiri Cancer Hospital; 2nd Propaedeutic Department of Internal Medicine, Oncology Section, University General Hospital Attikon; 1st Radiology Department, Areteion Hospital, Athens University Medical School; Department of Urology, Laikon Hospital, Athens University Medical School, Athens; Department of Urology, Papageorgiou Hospital, Aristotle University of Thessaloniki School of Medicine, Thessaloniki; Department of Urology, Sismanoglion Hospital, Athens University Medical School, Athens; Section of Biostatistics, Hellenic Cooperative Oncology Group, Data Office, Athens; Second Department of Internal Medicine, Oncology Section, Hippokration Hospital, Athens, Greece
NDT_A_245911 1199..1206

1Departamento de Farmacogenética, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Mexico City, Mexico; 2Kinder und Jugend Psychiatrie, Klinikum Idar-Oberstein, Idar-Oberstein, Germany; 3Departamento de Imágenes Cerebrales, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Mexico City, Mexico; 4Dirección de Servicios Clínicos, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Mexico City, Mexico Purpose: Bipolar disorder (BD) is a condition associated with structural alterations in the prefrontal cortex (PFC); some genetic variants and mood stabilizer medications like lithium or valproate are associated with these changes. CACNA1C is a gene involved in BD pathology and brain function; carriers of the A allele of rs1006737 are reported to have increased risk for BD and increased cortical thickness (CT) in the PFC compared to noncarriers. Lithium is also associated with increased CT in the PFC of BD subjects compared to the ones on valproate. The influence of these treatments and gene variants over the PFC structure of Mexican subjects has not been explored. Therefore, we evaluate the effects of mood stabilizers and risk A allele of CACNA1C rs1006737 on the prefrontal cortical thickness of Mexican BD patients treated with lithium or valproate. Patients and Methods: A cross-sectional study of 40 BD type I euthymic adult outpatients (20 treated with lithium and 20 with valproate) who underwent a 3T T1-weighted 3D brain scan and genotyping for CACNA1C risk allele rs1006737 was conducted. We performed a cortical thickness analysis of the dorsolateral and orbitofrontal regions of the prefrontal cortex with BrainVoyager 20.6. The effects of treatment and gene variants were analyzed with a two-way multivariate analysis of covariance. Results: There was no association of CACNA1C risk allele rs1006737 with CT measures of both PFCs nor significant interaction between the genetic variant and treatment. Mood stabilizers reported the main effect on the CT measures of the right PFC of our sample. Patients on treatment with lithium showed higher mean CT on the right orbitofrontal cortex. Conclusion: We did not find any association between the prefrontal CT and CACNA1C risk A allele rs1006737 in BD Mexican patients treated with lithium or valproate. Our results suggest that mood stabilizers had the main effect in the CT of the right PFC.
on epithelial mesenchymal transformation and invasion of choriocarcinoma cells

1 on epithelial mesenchymal transformation and invasion of choriocarcinoma cells LU Yan-jie, LIU Shao-chen, HU Ya-tao, ZHANG Shu-ling, ZUO Yan-zhen, XU Qian, LIU Lei, LI Yu-hong Institute of Cancer Research, Chengde Medical College, Chengde, Hebei 067000, China Department of Clinical Laboratory, Chengde County Hospital, Chengde, Hebei 067400, China Corresponding author, E-mail: youngcheer2003@foxmail.com This work was supported by the Graduate Student Innovation Fund Project of Hebei Province in 2015(202); the Key Subjects in Universities and Colleges of Hebei Province of China (Pathology and Pathophysiology); and the Natural Science Foundation of Hebei Province (H2012406010) [Abstract] Objective To explore the role of TGF1 on epithelial mesenchymal transformation and invasion by promoting cancer stem cell marker CD133 expression in choriocarcinoma cells. Method Choriocarcinoma cells JEG-3 were cultured in vitro and incubated with TGF1 at different time and concentration, and the expression of CD133 protein and mRNA of EMT markers were detected by Western blotting and PCR respectively. The effect of TGF1 on invasive ability of JEG-3 cells were assessed with transwell method. Results TGF1 promoted the expression of cancer stem cell marker CD133, downregulated the epithelial marker E-cadherin, upregulated mesenchymal marker N-cadherin, and promoted invasion ability in choriocarcinoma cells. Conclusion TGF1 could promote stem cell property of cancer, EMT property, and invasive property of choriocarcinoma cells. [Key words] choriocarcinoma; transforming growth factor beta1; neoplastic stem cells; cadherins; cell movement TGF1
Wattis, Jonathan A.D. (2004) Similarity solutions of a Becker-Döring system with time-dependent monomer

We formulate the Becker-Döring equations for cluster growth in the presence of a time-dependent source of monomer input. In the case of size-independent aggregation and fragmentation rate coefficients we find similarity solutions which are approached in the large time limit. The form of the solutions depends on the rate of monomer input and whether fragmentation is present in the model; four distinct types of solution are found. PACS numbers: 64.60. -i, 82.60. Nh, 05.70. Fh, aggregation equations, coagulationfragmentation equations, self-similar behaviour.
DDDT_A_278342 4695..4701

1Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, People’s Republic of China; 2Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, People’s Republic of China Purpose: Postoperative acute kidney injury (AKI) is a frequent complication in elderly patients that increases morbidity and mortality. Approximately 1.7 million people die from AKI worldwide every year. Dexmedetomidine (Dex) is often used as an adjunct to multimodal analgesia. Our study investigated whether Dex could safely decrease the incidence of AKI in elderly patients undergoing major joint replacement. Methods: A single-center retrospective study was conducted in patients aged >65 years undergoing major joint replacement. Propensity score–matching analysis was used, and a total of 1,006 patients were matched successfully. The primary outcome was the incidence of postoperative AKI. Secondary outcomes included perioperative adverse complications, opioid consumption, time to extubation, and length of hospital stay. Results: Among the 1,006 patients included, postoperative AKI occurred in 9.3% (n=94). The Dex group (perioperative Dex infusion) had lower incidence of postoperative AKI than the control group (7.2% vs 11.5%, P=0.017). Compared with the control group, the Dex group had less opioid consumption (P<0.05), reduced time to extubation (P=0.004), and shorter length of hospital stay (P=0.001). The Dex group also showed higher incidence of bradycardia (20.1% vs 15.1%, P=0.038). There were no differences in intraoperative hypotension (19.5% vs 17.5%), postoperative nausea and vomiting (4.2% vs 5.4%), time in PACU (45.0±6.4 vs 45.5±6.2 minutes), or rate of ICU admission (9.7% vs 11.1%) between the Dex group and control group (All P>0.05). Conclusion: This retrospective study showed Dex infusion in elderly patients undergoing major joint replacement was associated with lower incidence of postoperative AKI, less opioid consumption, and shorter extubation time and hospital stay. However, the Dex group had higher incidence of bradycardia. We found no statistical differences in other perioperative adverse complications between the groups.
A pilot study towards the implementation of perceptual and motor adaptation in robots

— The aim of our study is to understand the perceptual and motor mechanisms of adaptation underlying human-robot interaction. Our long-term goal is to develop novel models of adaptation that could be implemented in robots to enhance human-robot collaboration. Realizing adaptive robots would be fundamental not only in the biomedical field for assistance and rehabilitation, but also in industrial settings to improve human-robot cooperation. In the current paper, we present a pilot experiment aimed at exploring perceptual and motor strategies adopted by participants who try to adapt their perception to that of a robot with different prior sensory experience.
Contextualizing the 2019–20 Kangaroo Island Bushfires: Quantifying Landscape-Level Influences on Past Severity and Recovery with Landsat and Google Earth Engine

The 2019-20 Kangaroo Island bushfires in South Australia burned almost half of the island. To understand how to avoid future severe ‘mega-fires’ and how vegetation may recover from 2019– 2020, we can utilize information from the bulk of historical fires in an area. Landsat time-series of vegetation change provide this opportunity, but there has been little analysis of large numbers of fires to build a landscape-level understanding and quantify drivers in an Australian context. In this study, we built a yearly cloud-free surface reflectance normalized burn ratio (NBR) time-series (1988–2020) using all available summer Landsat images over Kangaroo Island. Data were collected in Google Earth Engine and fitted with LandTrendr. Burn severity and post-fire recovery were quantified for 47 fires, with a new recovery metric facilitating comparison where fire frequency is high. Variables representing the current burn, fire history, vegetation structure, and topography were related to severity and yearly recovery with random forest and bivariate analysis. Results show that the 2019–20 bushfires were the most widespread and severe, followed by 2007–08. Vegetation recovers quickly, with NBR stabilizing ten years post-fire on average. Severity is most influenced by fire frequency, vegetation capacity and land use with more severe burns in nature conservation areas with dense vegetation and a history of frequent fires. Influence on recovery varied with time since fire, with initial (year 1–3) faster recovery observed in areas with less surviving vegetation. Later (year 6–10) recovery was most influenced by a variable representing burn year and further investigation indicates that precipitation increases in later post-fire years likely facilitated faster recovery. The relative abundance of eucalypt woodlands also has a positive influence on recovery in middle and later years. These results provide valuable information to land managers on Kangaroo Island and in similar environments, who should consider adjusting practices to limit future mega-fire risk and potential ecosystem shifts if severe fires become more frequent with climate change.
Breast cancer: The role of herbal medication

For centuries, herbs and plants have been used for medicinal purposes and as food as well. This review concerns about different types of plants that retain the immune stimulating and anti-tumor properties. Large variety of active phytochemicals such as carotenoids, flavonoids, ligands, polyphenolics, terpenoids, sulfides, lignans and plant sterols has been identified in different types of herbs. Different modes of action are used by these phytochemicals. They either activate a defensive enzyme, such as glutathione transferase, or they stop cells from proliferating. The biochemical properties of Allium sativum , Echinacea , Curcuma longa , Arctium lappa , Camellia sinensis , Panax ginseng , and Flax seed were the focus of this study. Withania somnifera , Amoora rohituka , Dysoxylum binectariferum , and Vaccinium macrocarpon extracts and juices are also used as anti-breast cancer remedies. These herbs and plants’ volatile oils and extracts hinder the synthesis of mevalonate, which reduces tumour growth and cholesterol synthesis.
Human Kallikrein-2, PSA and Free-PSA for Discrimination of Syrian Prostate Cancer from Benign Disease

Prostate cancer is the most prevalent cancer and the second leading cause of cancer mortality among men in the western world. In Middle East countries it may be less common; but is still one of the most common cancers in men with elevated incidence especially in Lebanon and Syria (Salim et al., 2009; Shamseddin et al., 2014). Take into consideration the possibility associations between Prostate Cancer with genetic, familial, environmental and other factors, also the effect of different measures or detection programs applied in the different countries and the awareness variation of people toward these programs (Boyle et al., 2003; Arafa et al., 2012; Ezzi et al., 2014; Baade et al., 2015; Choubey et al., 2015) The prostate cancer had few specific symptoms in its early stage, for that reason, detecting cancer holds several difficult. The standard practice of screening for prostate cancer includes PSA, with or without digital rectal exam test (DRE), starting at the age of 50 years in all males or
P198. Faecal calprotectin level correlates well with balloon-assisted endoscopy and computed tomography enterography findings in small bowel Crohn’s disease

blood cell (WBC) count (r = -0.058, p = 0.496). Moreover, there was a trend for correlation between CDAI score and 6-TGN level, but it did not reach statistical significance(r = -0.163, p = 0.055). Conclusions: The 6-TGN levels within adequate therapeutic range are associated with a significantly higher chance of being in remission status. The monitoring of 6-TGN concentration may be helpful in the management of CD in adult Korean patients.
Embryonic Phases of Hard Composites: A Review

The incremental advancement in composites revolutionized the whole world in the 20 th century. Fundamental features of composite materials are high strength, light weight, fatigue resistance, high stiffness, fire resistance, flexibility and thermal resistance. Four generations of composites are the backbone in the development of composites i.e. Glass Fibers Reinforced Polymers (GFRP’S), high performance composites, synergy of properties of materials, hybrids and Nano composites. Bio-composites, shape memory composites, soft magnetic composites and flexible composites initially grasped the main focus of the whole world. Different manufacturing techniques i.e. hand lay-up, forming, vacuum bad molding and pultrusion method are utilized for composite fabrication. Composite materials utilized everywhere i.e. aerospace; wind power generation, offshore applications, telecommunication and industrial fields etc. huge flexibility, light weight and high strength make composite materials most suitable materials for aerospace applications. In this review paper, possible combinations, classifications and important development phases have been discussed. In addition, detailed features and characteristics of matrix and reinforcement materials and their categorization as composites have been listed.
Impact of routine cryptococcal antigen screening and targeted pre-emptive fluconazole therapy in antiretroviral naive HIV-infected adults with less than 100 CD 4 cells/μL: a systematic review and meta-analysis

Cryptococcal antigen (CrAg) screening and targeted naive HIV-infected adults with less than 100 CD 4 cells/μL seems promising to reduce the burden of cryptococcal meningitis (CM). We searched MEDLINE, EMBASE, and Web of Science and used random-effect meta-analysis to assess the prevalence of blood CrAg-positivity (31 studies; 35,644 participants) and asymptomatic CM in CrAg-positives, incidence of CM and all-cause mortality in screened participants. Pooled prevalence of blood CrAg-positivity was 6% (95%CI: 5 – 7) and asymptomatic CM in CrAg-positives was 33% (95%CI: 21 – 45). Incidence of CM without pre-emptive fluconazole was 21.4% (95%CI: 11.6 – 34.4) and 5.7% (95%CI: 3.0 – 9.7) with pre-emptive fluconazole initiated at 800 mg/day. In CrAg-positives, post-screening lumbar puncture prior to initiating pre-emptive fluconazole at 800 mg/day further reduced incidence of CM to null and showed some survival benefits. However, all-cause mortality remained significantly higher in CrAg-positives than CrAg-negatives: RR: 2.2 (95%CI: 1.7 – 2.9, p<0.001).
SHORT COMMUNICATION COMPOSITIONAL AND BACTERIOLOGICAL QUALITY OF HEAT TREARTED MILK MARKETED IN NAMIBIA

Production of fresh farm milk in Namibia is low due to the arid climate that prevails in the country, low pasture availability and a high milk demand. This scenario has forced Namibia to import dairy products to meet its needs from neighbouring South Africa, South America and Europe. Namibia imports different brands of fresh full cream milk including Parmalat, Clover, Super milk to add on to its own brands such as Namdairies, UNAM milk and others from private and commercial farms in bulk or in plastic sachets and bottles. Generally, milk quality is determined by its safety, shelf life and nutrition. The presence of different brands of milk makes it difficult for milk consumers in Namibia to choose the brand they would prefer including assessing their value for money. The aim of the project was to determine the quality of full cream milk brands marketed in Namibia by assessing their proximate composition, titratable acidity, pH, protein stability, total plate count and Coliform bacteria as indicator bacteria for the presence of pathogens, using standard dairy procedures and AOAC methods. The results of the study indicated that all imported brands of pasteurised full cream milk in Namibia were not significantly different from each other and were safe and stable, and nutritionally of good quality. They were practically the same in composition and bacteriological quality. Butterfat ranged from 3.4 to 3.7%, density from 1.029 to 1.032g/cm, total solids from 12.3 to 13.0%, solids-not-fat from 8.6 to 9.7%, moisture content from 87.10 to 87.70%, acidity as lactic acid from 0.15 to 0.17% and pH from 6.4 to 6.7. The total plate count (TPC) ranged from 0 to 40 cfu/cm and there were no Coliform bacteria found in all the brands. The local products were not statistically different in composition from imported brands, with fat ranging from 3.3 to 3.7%, density from 1.029-1.032g/cm, TS from 12.3-12.9% and SNF from 8.6-9.4% though they were not standardized but heat treated. The total aerobic counts and Coliforms were the same as those of imported milk brands. The statistical analysis showed that there were no significant differences at p<0.05 among the brands and between the brands of the imported and local milk in Namibia as they were all within the South African Standard Specifications. All the brands were, however, regarded as good, stable and safe for consumption.
Catalyst Heating Characteristics in the Traveling ‐ Wave Microwave Reactor

Traveling‐Wave Microwave Reactor (TMR) presents a novel heterogeneous catalytic reac‐ tor concept based on a coaxial waveguide structure. In the current paper, both modeling and exper‐ imental studies of catalyst heating in the TMR are presented. The developed 3D multiphysics model was validated from the electromagnetic and heat transfer points of view. Extrudes of silicon carbide (SiC) were selected as catalyst supports and microwave absorbing media in a packed‐bed configu‐ ration. The packed‐bed temperature evolution was in good agreement with experimental data, with an average deviation of less than 10%. Both experimental and simulation results show that the ho‐ mogeneous temperature distribution is possible in the TMR system. It is envisioned that the TMR concept may facilitate process scale‐up while providing temperature homogeneity beyond the in‐ trinsic restrictions of microwave cavity systems.
DDDT_A_322502 4275..4287

1Department of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, 266003, People’s Republic of China; 2Department of Oral Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266003, People’s Republic of China; 3Department of Endocrinology, The Third People’s Hospital of Qingdao Affiliated to Qingdao University, Qingdao, 266003, People’s Republic of China