Miracle-dz/UniFAR-FTUs huggingface dataset:
Psychological/Personal recovery and its correlates in patients with first episode psychosis

Background: Recovery in psychosis is a multidimensional construct. The numbers of studies specially focusing on the recovery aspects in patients with first episode psychosis (FEP) are limited, with no study from India. Further, no study has looked specifically into the variables that affect recovery process in patients with FEP and little is known about factors which influence recovery in patients with FEP. Aim: To evaluate psychological recovery and its correlates in patients with FEP, currently in clinical remission. Methodology: One hundred three patients of FEP in clinical remission were assessed on Recovery Assessment Scale (RAS), Positive and Negative Syndrome Scale for Schizophrenia, Calgary Depression Rating Scale for Schizophrenia, Negative Symptom Assessment 16, Rosenberg Self-esteem Scale, Social and Occupational Functioning Assessment Scale, the Alcohol, Smoking and Substance Involvement Screening Test, Beck Cognitive Insight Scale, Internalized Stigma of Mental Illness Scale, the Everyday discrimination Scale, Subjective Scale to Investigate Cognition in Schizophrenia and social cognitive deficits in theory of mind was evaluated on the Social Cognition Rating Tools in Indian Setting-Theory of Mind. The needs of the participants were assessed on the Camberwell Assessment of Needs – Research version and Supplemental Assessment of Needs. Coping, social support, medication adherence were also assessed by standardized scales. Results: The mean weighted score was highest for goal and success orientation subscale followed by seeking and relying on social support, personal confidence and hope, overcome the illness and awareness and control over the illness as assessed by 41 items of the RAS. The main factors identified to affect psychological recovery in patients with FEP were duration of untreated psychosis, greater psychopathology, lower self-esteem, total unmet need, feeling of discrimination and stigmatization. Conclusion: The present study suggests that treatment of FEP should be started at the earliest and issues such as residual psychopathology, lower self-esteem, total unmet need, feeling of discrimination and stigma during the remission phase should be addressed by psychosocial interventions to promote psychological recovery in patients with first episode psychosis.
Parvalbumin-Positive Interneurons in the Medial Prefrontal Cortex Regulate Stress-Induced Fear Extinction Impairments in Male and Female Rats

Stress has profound effects on fear extinction, a form of learning that is essential to behavioral therapies for trauma-related and stressor-related disorders. Recent work reveals that acute footshock stress reduces medial prefrontal cortex (mPFC) activity that is critical for extinction learning. Reductions in mPFC activity may be mediated by parvalbumin (PV)-containing interneurons via feedforward inhibition imposed by amygdala afferents. To test this hypothesis, footshock stress-induced Fos expression was characterized in PV+ and PV– neurons in the prelimbic (PL) and infralimbic (IL) cortices. Footshock stress increased the proportion of PV+ cells expressing Fos in both male and female rats; this effect was more pronounced in IL compared with PL. To determine whether PV+ interneurons in the mPFC mediate stress-induced extinction impairments, we chemogenetically silenced these neurons before an immediate extinction procedure in PV-Cre rats. Clozapine-N-oxide (CNO) did not affect conditioned freezing during the extinction procedure. However, CNO exacerbated extinction retrieval in both male and female rats with relatively high PL expression of designer receptors exclusively activated by designer drugs (DREADD). In contrast, in rats with relatively high IL DREADD expression, CNO produced a modest facilitation of extinction in the earliest retrieval trials, but in male rats only. Conversely, excitation of IL PV interneurons was sufficient to impair delayed extinction in both male and female rats. Finally, chemogenetic inhibition of IL-projecting amygdala neurons reduced the immediate extinction deficit in male, but not female rats. These results reveal that PV interneurons regulate extinction learning under stress in a sex-dependent manner, and this effect is mediated by amygdaloprefrontal projections. SIGNIFICANCE STATEMENT Stress significantly impairs the memory of fear extinction, a type of learning that is central to behavioral therapies for trauma-based and anxiety-based disorders (e.g., post-traumatic stress disorder). Here we show that acute footshock stress recruits parvalbumin (PV) interneurons in the medial prefrontal cortex (mPFC) of male and female rats. Silencing mPFC PV interneurons or mPFC-projecting amygdala neurons during immediate extinction influenced extinction retrieval in a sex-dependent manner. This work highlights the role for PV-containing mPFC interneurons in stress-induced impairments in extinction learning.
A multi-ancestry genome-wide meta-analysis, fine-mapping, and target gene prioritization to characterize the genetic architecture of adiponectin.

Previous genome-wide association studies (GWAS) for adiponectin, a complex trait linked to type 2 diabetes and obesity, identified >20 associated loci. However, most loci were identified in populations of European ancestry, and many of the target genes underlying the associations remain unknown. We conducted a multi-ancestry adiponectin GWAS meta-analysis in up to 46,434 individuals from the METSIM cohort and the ADIPOGen and AGEN consortiums. We combined study-specific association summary statistics using a fixed-effects, inverse variance-weighted approach. We identified 22 loci associated with adiponectin (P < 5e-8), including 15 known and 7 previously unreported loci. Among individuals of European ancestry, GCTA-COJO identified 14 additional distinct signals at the ADIPOQ, CDH13, HCAR1, and ZNF664 loci. Leveraging the multi-ancestry data, FINEMAP + SuSiE identified 46 causal variants (PP>0.9), which also exhibited potential pleiotropy for cardiometabolic traits. To prioritize target genes at associated loci, we propose a combinatorial likelihood scoring formalism (GPScore) based on measures derived from 11 gene prioritization strategies and the physical distance to the transcription start site. With GPScore, we prioritize the 30 most probable target genes underlying the adiponectin-associated variants in the multi-ancestry analysis, including well-known causal genes (e.g., ADIPOQ, CDH13) and novel genes (e.g., CSF1, RGS17). Functional association networks revealed complex interactions of prioritized genes, their functionally connected genes, and their underlying pathways centered around insulin and adiponectin signaling, indicating an essential role in regulating energy balance in the body, inflammation, coagulation, fibrinolysis, insulin resistance, and diabetes. Overall, our analyses identify and characterize adiponectin association signals and inform experimental interrogation of target genes for adiponectin.
Identifying core MRI sequences for reliable automatic brain metastasis segmentation

Background: Many automatic approaches to brain tumor segmentation employ multiple magnetic resonance imaging (MRI) sequences. The goal of this project was to compare different combinations of input sequences to determine which MRI sequences are needed for effective automated brain metastasis (BM) segmentation. Methods: We analyzed preoperative imaging (T1-weighted sequence without and with contrast- enhancement (T1/T1-CE), T2-weighted sequence (T2), and T2 fluid-attenuated inversion recovery (T2-FLAIR) sequence) from 333 patients with BMs from six centers. A baseline 3D U-Net with all four sequences and six U-Nets with plausible sequence combinations (T1-CE, T1, T2-FLAIR, T1-CE+T2-FLAIR, T1-CE+T1+T2-FLAIR, T1- CE+T1) were trained on 239 patients from two centers and subsequently tested on an external cohort of 94 patients from four centers. Results: The model based on T1-CE alone achieved the best segmentation performance for BM segmentation with a median Dice similarity coefficient (DSC) of 0.96. Models trained without T1-CE performed worse (T1-only: DSC = 0.70 and T2- FLAIR-only: DSC = 0.72). For edema segmentation, models that included both T1-CE and T2-FLAIR performed best (DSC = 0.93), while the remaining four models without simultaneous inclusion of these both sequences reached a median DSC of 0.81-0.89. Conclusions: A T1-CE-only protocol suffices for the segmentation of BMs. The combination of T1-CE and T2-FLAIR is important for edema segmentation. Missing either T1-CE or T2-FLAIR decreases performance. These findings may improve imaging routines by omitting unnecessary sequences, thus allowing for faster procedures in daily clinical practice while enabling optimal neural network-based target definitions.
Learning in Monotone Bayesian Games

This paper studies learning in monotone Bayesian games with one-dimensional types and finitely many actions. Players switch between actions at a set of thresholds. A learning algorithm under which players adjust their strategies in the direction of better ones using payoffs received at similar signals to their current thresholds is examined. Convergence to equilibrium is shown in the case of supermodular games and potential games.
Evaluation of the efficacy and safety of hyaluronic acid and supplemented with amino acids, and glutathione or colin, for the prevention and treatment of wrinkles on the face, neck, décolleté and hands.

OBJECTIVE Hyaluronic acid has been used for a long time as a biorevitalizer to treat skin aging both in single formulation and in association with other compounds such as amino acids or vitamins. The purpose of this study was to evaluate the efficacy and safety of class III medical devices SKIN B, SKIN R, SKIN OX, SKIN COLIN, for the treatment of wrinkles on the face, neck, décolleté and hands. These medical devices are all based on hyaluronic acid and supplemented with amino acids (SKIN B and SKIN R) and glutathione (SKIN OX) or colin (SKIN COLIN). This gives broader possibilities to the aesthetic surgeon for personalization and pharmacological diversification based on the patient's deficits to treat. PATIENTS AND METHODS A total of 60 subjects affected by skin damage such as rhytidids, dehydration, reduced sebum production and skin hypoxia were enrolled. The patients were treated with a session of mesotherapy every 7 days for 30 days, followed by other 2 sessions every 15 days for 30 days and the follow-up on day 90. The primary efficacy endpoint was evaluated by means of a 0-10 visual analog scale at day 60; the secondary efficacy endpoint was evaluated by means of a 0-10 visual analog scale at day 90. RESULTS All patients completed the 3-month follow-up. Treatment with SKIN B, SKIN OX, SKIN COLIN and SKIN R medical devices for the prevention and treatment of wrinkles on the face, neck, décolleté and hands were associated with favorable and positive results. A clear reduction of wrinkles has been clinically observed with improvement in the texture, brightness, and turgor of the skin. No adverse events were reported. CONCLUSIONS The medical devices SKIN B, SKIN SKIN OX, SKIN COLIN produce an aesthetic improvement in patients affected by skin defects, when administered by mesotherapy technique. This study confirms the safety and efficacy of the medical devices based on hyaluronic acid supplemented with amino acids and glutathione or colin.
Experience and acceptability for HPV self-sampling among women in Jiangsu province, China: a cross-sectional survey

Abstract This aim of this study was to investigate women’s knowledge about HPV along with their experience and acceptability of self-sampling in Jiangsu province, China. A total of 862 women aged 25–63 years old from Jiangsu province who purchased an HPV self-sampling test kit were invited to complete a questionnaire designed by the authors. Participants had high acceptability for HPV self-sampling with a mean score of 4.2 (95% [CI], 4.1–4.22) out of 5 points. 27% of participants preferred clinician-sampling, 33% preferred self-sampling, other 40% expressed no preference. Women with good knowledge about HPV and with a good experience with HPV self-sampling were more acceptable for self-sampling (P < 0.05). The biggest concern about HPV self-sampling of the participants includes ‘specimens’ spoilage’, ‘incorrect sampling’, ‘can’t get results in time’, and so on. HPV self-sampling can be used to improve cervical cancer screening coverage and participation rates in China. IMPACT STATEMENT Cancer screening and can be an alternative primary screening for cervical cancer. •What the results of this study add? This study adds new findings about Chinese women’s experience and acceptability of HPV self-sampling. We found that most women had high acceptability for HPV self-sampling in Jiangsu province, China, and high knowledge about HPV as well as good •What is already known on this subject? HPV self-sampling testing was proven to be useful for improving the uptake rate of cervical experience of self-sampling can improve the acceptability for self-sampling in women. •What the implications are of these findings for clinical practice and/or further research? Further research should assess the acceptability of women with less education or who never screened.
Important considerations regarding the widespread use of doxycycline chemoprophylaxis against sexually transmitted infections

Abstract Rates of sexually transmitted infections (STIs) continue to rise across the world and interventions are essential to reduce their incidence. Past and recent studies have indicated this may be achieved using doxycycline post-exposure prophylaxis (PEP) and this has sparked considerable interest in its use. However, many unanswered questions remain as to its long-term effects and particularly potentially negative impact on human microbiomes and antimicrobial resistance among STIs, other pathogens, and commensals. In this review, we discuss seven areas of concern pertaining to the widespread use of doxycycline PEP.
1,25-Dihydroxvitamin D3 attenuates the damage of human immortalised keratinocytes caused by Ultraviolet-B

Abstract Objective Ultraviolet-B (UVB) radiation is an important factor in causing skin damage. The study is to explore whether 1,25-Dihydroxvitamin D3(1,25(OH)2D3) will attenuate the damage of human immortalised keratinocytes (HaCaT) cells caused by UVB and relevant underlying mechanisms. Methods CCK-8 was employed to determine the UVB irradiation intensity and 1,25(OH)2D3 concentration. Western blot was used to detect the expression of NF-κB, Caspase9, Caspase3, Bax, Bcl2, FADD, CytC, Beclin-1; Flowcytometry was applied to measure the production of ROS. Results The concentration of 1,25(OH)2D3 used in the study was 100 nM and the UVB irradiation intensity was 20 mJ/cm2. Compared with the HaCaT cells irradiated with UVB, the HaCaT cells that were pre-treated with 1,25(OH)2D3 had lower production of ROS, lower expression of NF-κB, Caspase9, Caspase3, Bax, FADD, CytC and Beclin-1(P < 0.05). Conclusion 1,25(OH)2D3 could inhibit the development of oxidative stress and apoptosis in HaCaTs triggered by UVB. This inhibition might be achieved through the suppression of mitochondria-modulated apoptosis and autophagy. Vitamin D may be a potential UVB protective component.
Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells.

Fluorescent protein (FP) tagging is a key method for observing protein distribution, dynamics, and interaction with other proteins in living cells. However, the typical approach using overexpression of tagged proteins can perturb cell behavior and introduce localization artifacts. To preserve native expression, fluorescent proteins can be inserted directly into endogenous genes. This approach has been standard in yeast for decades, and more recently in invertebrate model organisms with the advent of CRISPR/Cas9. However, endogenous FP tagging has not been widely used in mammalian cells due to inefficient homology-directed repair. Recently, the CRISPaint system has utilized non-homologous end joining for efficient integration of FP tags into native loci, but it only allows C-terminal knock-ins. Here, we enhanced the CRISPaint system by introducing new universal donors for N-terminal insertion and for multi-color tagging with orthogonal selection markers. We adapted the procedure for mouse embryonic stem cells, which can be differentiated into diverse cell types. Our protocol is rapid and efficient, enabling live imaging in less than 2 weeks post-transfection. These improvements increase the versatility and applicability of FP knock-in in mammalian cells.
Opium alkaloids, biosynthesis, pharmacology and association with cancer occurrence

Papaver somniferum L. (Family: Papaveraceae) is a species well known for its diverse alkaloids (100 different benzylisoquinoline alkaloids (BIAs)). L-tyrosine serves as a precursor of several specific metabolites like BIAs. It has been used as an antitussive and potent analgesic to alleviate mild to extreme pain since ancient times. The extraction of pharmaceutically important alkaloids like morphine and codeine from poppy plant reflects the need for the most suitable and standard methods. Several analytical and extraction techniques have been reported in open literature for morphine, codeine and other important alkaloids which play a vital function in drug development and drug discovery. Many studies suggest that opioids are also responsible for adverse effects or secondary complications like dependence and withdrawal. In recent years, opium consumption and addiction are the most important risk factors. Many evidence-based reviews suggest that opium consumption is directly linked or acts as a risk factor for different cancers. In this review, we highlight significant efforts related to research which have been done over the past 5 decades and the complete information on Papaver somniferum including its phytochemistry, pharmacological actions, biosynthetic pathways and analytical techniques of opium alkaloid extraction and the link between opium consumption and cancer-related updates.
Penerapan Algoritma Genetika untuk Optimasi Parameter pada Support Vector Machine untuk Meningkatkan Prediksi Pemasaran Langsung

Abstract : Pemasaran langsung adalah proses mengidentifikasi potensi pembeli produk tertentu dan mempromosikan produk dengan sesuai. pelaksanaan pemasaran langsung dari waktu ke waktu menghasilkan data dan informasi dalam bentuk laporan yang perlu di analisis oleh manajer dalam rangka mendukung keputusan. Namun itu adalah tugas yang sulit bagi manusia untuk menganalisis data yang kompleks yang luas. Kesulitan ini menyebabkan perkembangan teknik intelejen bisnis, yang bertujuan mengklasifikasi pengetahuan yang berguna untuk mendukung pengambilan keputusan. Metode support vector machine mampu mengatasi masalah yang berdimensi tinggi, mengatasi masalah klasifikasi dan regresi dengan linier ataupun nonlinier kernel yang dapat menjadi satu kemampuan algoritma pembelajaran untuk klasifikasi serta regresi, namun support vector machine memiliki masalah dalam pemilihan parameter yang sesuai. Untuk mengatasi masalah tersebut di perlukan metode algoritma genetika untuk pemilihan parameter yang sesuai pada metode support vector machine. Beberapa eksperimen dilakukan untuk mendapatkan akurasi yang optimal. Hasil penelitian menunjukan, eksperimen dengan menggunakan metode support vector machine dan algoritma genetika yang digunakan untuk melakukan optimasi parameter C, γ dan e dengan tiga jenis kernel. Kernel pertama tipe kernel dot dengan akurasi sebesar 85,59%, AUC sebesar 0,911 yang kedua tipe kernel radial dengan akurasi sebesar 98.89%, AUC sebesar 0,981 dan yang ketiga dengan tipe kernel Polynomial dengan akurasi sebesar 98.67% dan AUC sebesar 0.938. Hasil eksperimen tersebut menunjukan pengujian data set menggunakan penerapan algoritma genetika pada support vector machine menunjukan hasil yang lebih akurat untuk prediksi pemasaran langsung.
Large pedunculated colorectal polyps: more than meets the eye

vention of colorectal cancer (CRC) hinges on the identification and removal of precancerous polyps [1]. It is now firmly established that the overwhelming majority of all colorectal polyps can be resected endoscopically, thereby avoiding surgery, which is associated with greater risks and is more costly [2]. Nevertheless, post-colonoscopy colorectal cancers occur among patients who have undergone one or more colonoscopies, where both missed and incompletely resected polyps are recognized risk factors for this undesirable outcome [3].
Biological Characteristics and Resource Utilization of Tagetes minuta L.

Abstract: Tagetes minuta L. is native to the temperate grasslands and relatively high-altitude areas of South America. In 1990, it was first discovered in the Beijing Botanical Garden in China. In 1994, Pema Dawa introduced the species as a Tibetan medicine from the mountains of northern India to his courtyard in Gangjiang Village, Wolong Town, Milin County. Around 2010, scholars discovered that Tagetes minuta L. was widely invasive, which aroused extensive social concern. Featuring small weight, large number, and awns, Tagetes minuta L. , is easy to spread and has a high seed germination rate, with 20% of the reproductive investment. The plant is tolerant of barrenness and drought and adapts to climates with large diurnal temperature differences and strong light conditions. Rich in aromatic substances and essential oils, the leaves and flowers serve medicinal, food, flavoring, health care and cosmetic purposes. Thiophene in the roots and bioactive components in the essential oil have anti-inflammatory, sterilizing, insect-repellent, and fly control effects, with significant allelopathy on native species. The biological and ecological characteristics of Tagetes minuta L. lay the material basis for its population spread. According to Tibetan folklore, we suggest using physical methods or biological control techniques for ecological control and strengthening the research of development and application to guide its economic benefits in Tibetan Buddhist supplies.
Microbiological Approach for Leaching Out Metallic Elements from Electric and Electronic Waste

Abstract: After the computer and mobile revolution, electric and electronic waste had become a serious threat to urban and rural communities equally. Prevention of the hazardous exposure and proper management are challenging in developing nations. One way to turn the crisis to opportunity is to extract metals from this Waste Electronic and Electric Equipment (WEEE) is making waste into a source of metal ores. The involvement of microbes in this technology could increase the boons by being an eco-friendly technique for reducing the hazardous nature. This article reviews the mechanisms involved in the process of bioleaching, the microorganisms employed, methods used and various developments as well as limitations along with recent advances and future prospects of the process of bioleaching of metals from WEEE.
Large third-order nonlinear refractive index coefficient based on gold nanoparticle aggregate films

Au nanoparticles show large third-order nonlinear effect and ultra-fast response. Here a high nonlinear aggregate film based on self-assembled gold nanoparticles is reported and its third-order nonlinear refractive index coefficient is measured by Z-scan experiment. The third-order nonlinear refractive index coefficient of the Au nanoparticle aggregate film (γ1 = 9.2 × 10−9 cm2/W) is found to be larger than that of an 8-nm-thick sputtered Au film (γ2 = 6.5 × 10−9 cm2/W). This large nonlinear effect can be attributed to the strong field enhancement due to localized plasmon resonances between Au nanoparticles. The result shows that the self-assembled Au nanoparticle aggregate film could be a promising candidate as a third-order nonlinear optical material.