Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon

The Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for vivax malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the pvcyrpa genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the pvcyrpa gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct P. vivax endemic areas worldwide.
Mapping of Moist Snow and Percolation Zones Through Sentinel-2

The purpose of this work consists of an application of remote sensing indices such as NDSI and NDSW and geoprocessing analyzes to map an occurrence of water, percolation and moist snow zones, as well as an identification of the characteristic altitudes of each snow zone mapped in the Dundee Island located on the Antarctic Peninsula. For this, a Sentinel-2 image representative of an ablation period corresponding to the end of summer of 2016 was used. As a result an interaction between the NDSI and NDSW indexes allowed a distinction and delimitation of the different zones of snow. As geoprocessing analyzes that involved an overlapping of the land cover classification from the indices, with the altimetric data from the Digital Elevation Model ASTER GDEM, allowed an identification of some bands altimetry characteristic of each zoning. Data base analysis was identified with 17.87% of the total area of the island for the class of moist snow and 81.15% as zone of snow in percolation. However, these results prove the effectiveness of the methodology used based on images of the MSI sensor of the Sentinel-2 satellite in the mapping of the several snow areas in the study area, bringing together geoprocessing tools.
Expression of FLRT2 in Postnatal Central Nervous System Development and After Spinal Cord Injury

Fibronectin and leucine-rich transmembrane (FLRT) proteins are necessary for various developmental processes and in pathological conditions. FLRT2 acts as a homophilic cell adhesion molecule, a heterophilic repulsive ligand of Unc5/Netrin receptors, and a synaptogenic molecule; the last feature is mediated by binding to latrophilins. Although the function of FLRT2 in regulating cortical migration at the late gestation stage has been analyzed, little is known about the expression pattern of FLRT2 during postnatal central nervous system (CNS) development. In this study, we used Flrt2-LacZ knock-in (KI) mice to analyze FLRT2 expression during CNS development. At the early postnatal stage, FLRT2 expression was largely restricted to several regions of the striatum and deep layers of the cerebral cortex. In adulthood, FLRT2 expression was more prominent in the cerebral cortex, hippocampus, piriform cortex (PIR), nucleus of the lateral olfactory tract (NLOT), and ventral medial nucleus (VM) of the thalamus, but lower in the striatum. Notably, in the hippocampus, FLRT2 expression was confined to the CA1 region and partly localized on pre- and postsynapses whereas only few expression was observed in CA3 and dentate gyrus (DG). Finally, we observed temporally limited FLRT2 upregulation in reactive astrocytes around lesion sites 7 days after thoracic spinal cord injury. These dynamic changes in FLRT2 expression may enable multiple FLRT2 functions, including cell adhesion, repulsion, and synapse formation in different regions during CNS development and after spinal cord injury.
Morphokinetics of Porcine and Bovine Embryos and Anomalies in Their Development

The objective of this study was to evaluate the kinetics of bovine and porcine embryos that failed or developed to the blastocyst stage and anomalies in cleavage using a time-lapse monitoring system. The timing of early cleavages and their duration were similar for bovine and porcine embryos that developed to the blastocyst stage. There were differences in the time of first and second cell division of the bovine embryos that developed and those that did not develop to blastocyst stage (P=0.004 and P=0.002), respectively. Similarly, in case of porcine embryos such difference was observed only in the time of first cleavage (P=0.0001). Direct cleavage from 1-cell to 3 cells occurred in 13.47% and to more than 3 cells in 3.37% of porcine embryos whereas to 3-cells occurred in 4.23% of bovine embryos. The reverse cleavage was observed in 4.33% of porcine and 8.45% of bovine embryos. Conclusion: Our study showed: 1) The similarities in timing and duration of early cleavages of bovine and porcine embryos during development to the blastocyst stage, 2) Differences in morphokinetic parameters between bovine and porcine embryos developing or non-developing to the blastocyst, 3) Anomalies in cleaving of in vitro developing bovine and porcine embryos.
Farmer Perceptions of Adopting Novel Legumes in Traditional Maize-Based Farming Systems in the Yucatan Peninsula

Intercropping constitutes the traditional farming system practice used in various forms for maize production in the Yucatan peninsula. Although practiced for centuries, problems persist with competition for water, nutrients and light between crop species in traditional farming systems. Furthermore, little is known about farmers’ perceptions regarding changes to traditional maize-legume intercropping systems and their interest in novel crop adoption to increase yields in the system while maintaining the practice. The objective of this study was to investigate the maize-based traditional cropping system by assessing the underlying motives and concepts of farmers to practice intercropping in the Yucatan Peninsula and to examine the association between farmers’ level of knowledge about legumes and decisions to adopt intercropping and related practices therein. Farmer surveys were conducted in nine different regions of the Yucatan Peninsula. We selected Xoy, Euan, Muna, Mama, Tahdziú (Yucatan), Becal, Hecelchacam, Dzitbalché and San Antonio Sahcabchén (Campeche) which are representative of agroecological small-scale farming systems. We used a mixed methods case study analysis involving key informant interviews in eight associations of farmers. A sample frame with 73 farmers was selected in total during February 2021 and April 2021. Basic information such as land use, labor inputs, agricultural production and farmer’s perceptions regarding their intercropping systems were collected. Our research shows that the primary motives for intercropping were due to the ability of intercropping to offer a more diversified range of food for human and animal consumption, as well as to take advantage of different harvest periods that this practice offers. The majority of respondents were likely to favor the idea of introducing new legume species in their maize-based cropping systems. Factors such as the type of cropping system (i.e., intercropping or monocropping), access to water and level of knowledge about legumes influenced their decision to adopt intercropping in their farming systems considerably. This paper contributes to the knowledge on the current state and farmers’ perceptions of intercropping systems in the Yucatan Peninsula.
A Method to Automate the Prediction of Student Academic Performance from Early Stages of the Course

The objective of this work is to present a methodology that automates the prediction of students’ academic performance at the end of the course using data recorded in the first tasks of the academic year. Analyzing early student records is helpful in predicting their later results; which is useful, for instance, for an early intervention. With this aim, we propose a methodology based on the random Tukey depth and a non-parametric kernel. This methodology allows teachers and evaluators to define the variables that they consider most appropriate to measure those aspects related to the academic performance of students. The methodology is applied to a real case study obtaining a success rate in the predictions of over the 80%. The case study was carried out in the field of Human-computer Interaction.The results indicate that the methodology could be of special interest to develop software systems that process the data generated by computer-supported learning systems and to warn the teacher of the need to adopt intervention mechanisms when low academic performance is predicted.
A Simplified Vehicle Dynamics Model for Motion Planner Designed by Nonlinear Model Predictive Control

Motion planning by considering it as an optimal problem is an effective and widely applicable method. Its comprehensive performance greatly depends on the vehicle dynamics model, which is highly coupled and nonlinear, especially under the dynamical scenarios and causes much more consumption of computation resources for the numerical optimization. To increase the real time performance of the motion planner designed by nonlinear model predictive control (NMPC), a unified and simplified vehicle dynamics model (SDM) is presented to make a balance between the accuracy and complexity for dynamical driving scenarios. Based on the statistical analysis results of naturalistic driving conditions, a unified nonlinear vehicle dynamics model is set up, which considers the tyre cornering characteristic and is also applicable to conditions with large turning angle. After the validation of this coupled dynamics model (CDM) by comparisons with other widely used models under a variety of conditions, the coupling effect is analyzed according to the transfer functions, which are obtained by linearizing CDM at equilibrium points. Furthermore, SDM is derived by ignoring the weak part of the coupling effect. The accuracy of SDM is validated by several comparative studies with other models and it is further applied to design a motion planner by NMPC to validate its contribution on the performance improvement under dynamical driving conditions.
Dinámica de éxito del crowdfunding no financiero en un contexto emergente: el caso de Colombia

El objetivo de esta investigación fue analizar factores asociados al éxito de las campañas de crowdfunding no financiero en economías emergentes mediante el estudio de 372 proyectos publicados entre 2012 y 2017 en cinco plataformas colombianas. Se utilizó un modelo de regresión lineal múltiple generalizado y uno de regresión logística para evaluar la variable dependiente, tanto desde el punto de vista cuantitativo como cualitativo. Las características de los proyectos evaluados se incluyeron como variables explicativas. Los resultados demuestran que la plataforma utilizada, la ubicación del creador, el número de financiadores y las actualizaciones son factores asociados al éxito. Específicamente, en contextos con poco desarrollo de tecnologías de financiamiento, es muy importante el papel de la plataforma como intermediario entre creador y financiador.
Design of the LAN Network of Hospital Comandante Manuel Piti Fajardo

The design of the network of a health institution is a complicated task due to all the aspects that it encompasses, to satisfy the consumption needs of digitized services, using minimal time and at the lowest possible cost. The Manuel Piti Fajardo Hospital, according to the scope of health services it provides, has a system for Hospital Management, the Galen Clinica. This system requires a well-structured network design, with the appropriate equipment that responds quickly and efficiently to the traffic generated in the network. In line with this objective, a cost-benefit study was carried out, after applying the LAN design methodology and calculating approximately the traffic generated on the network in the main departments of the hospital, whether it be the Matrix or the Unit. Surgical and Imaging. With this, the result was to locate the network backbone, and determine the network components that should be replaced according to the financial budget of the hospital, a better response to the requests made by users and according to the evolution of technologies. of information and communications.
Neuromelioidosis Presenting as Bells Palsy in a Child

Annals of Indian Academy of Neurology ¦ Volume 25 ¦ Issue 2 ¦ March-April 2022 302 Sir, Melioidosis is a tropical infection caused by Burkholderia pseudomallei and is endemic in South East Asia and Northern Australia. [1] Central nervous system melioidosis is rare in children and only a few cases have been reported in the literature. [2] It can present as meningoencephalitis, cerebral abscess, myelitis, cranial nerve palsies, and can mimic Guillain Barre Syndrome. [3] We report an interesting case of neuro melioidosis presenting as lower motor neuron type of facial palsy who proceeded to develop third nerve palsy, hemiparesis, and meningeal signs.
MiRNAs Expression Profiling in Raw264.7 Macrophages after Nfatc1-Knockdown Elucidates Potential Pathways Involved in Osteoclasts Differentiation

Simple Summary Bone diseases are a worldwide health public problem, and their management has required extensive studies on bone homeostasis. Among all the various mechanisms involved, understanding those underlying osteoclastogenesis is of paramount importance. Here, we tried to elucidate the possible role of miRNAs differentially expressed in RANKL-stimulated RAW264.7 cells expressing the transcription factor NFATc1, the master regulator of osteoclasts generation, and in NFATc1-silenced RAW264.7 cells, which are depleted of NFATc1 by 80%. We performed miRNAs PCR array followed by bioinformatic analysis to discover new possible miRNAs and their targets involved in this process. The results were interesting and suggested that relatively unknown miRNAs (miR-880 and miR-295) control the phosphorylation/dephosphorylation of proteins/transcription factors such as ERK-p38 and NFATc1, by enzymes (DYRKs and DUSPs). In addition, our results confirmed the role of some miRNAs, already known for their involvement in the process of mature osteoclasts formation. This study contributes to a more complete overview of the early stages of osteoclast formation, including cell migration and fusion. Abstract Differentiation of macrophages toward osteoclasts is crucial for bone homeostasis but can be detrimental in disease states, including osteoporosis and cancer. Therefore, understanding the osteoclast differentiation process and the underlying regulatory mechanisms may facilitate the identification of new therapeutic targets. Hereby, we tried to reveal new miRNAs potentially involved in the regulation of early steps of osteoclastogenesis, with a particular focus on those possibly correlated with NFATc1 expression, by studying miRNAs profiling. During the first 24 h of osteoclastogenesis, 38 miRNAs were differentially expressed between undifferentiated and RANKL-stimulated RAW264.7 cells, while 10 miRNAs were differentially expressed between RANKL-stimulated cells transfected with negative control or NFATc1-siRNAs. Among others, the expression levels of miR-411, miR-144 and members of miR-29, miR-30, and miR-23 families changed after RANKL stimulation. Moreover, the potential role of miR-124 during osteoclastogenesis was explored by transient cell transfection with anti-miR-124 or miR-124-mimic. Two relatively unknown miRNAs, miR-880-3p and miR-295-3p, were differentially expressed between RANKL-stimulated/wild-type and RANKL-stimulated/NFATc1-silenced cells, suggesting their possible correlation with NFATc1. KEGG enrichment analyses showed that kinase and phosphatase enzymes were among the predicted targets for many of the studied miRNAs. In conclusion, our study provides new data on the potential role and possible targets of new miRNAs during osteoclastogenesis.
Comparative study between intrathecal dexmedetomidine and intrathecal magnesium sulfate in prevention of post spinal shivering in uroscopic surgery. (RCT)

Background: Hypothermia and shivering are associated common complications after spinal anesthesia especially in Magnesium sulphate and dexmedetomidine are the most effective adjuvants with least side effects. Our aim of the study is to compare the effect of intrathecal dexmedetomidine versus intrathecal magnesium sulfate in prevention of post spinal shivering. Methods: This prospective randomized, double-blinded controlled study was conducted at Kasr El-Aini Hospital on 105 patients scheduled for uroscopic surgeries. patients were randomly allocated into three groups using computerized generated random tables, Group C (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5 mg) +0.5 ml normal saline, Group M (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5 mg) +25 mg magnesium sulfate in 0.5 ml saline and Group D (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5mg) + 5 μg dexmedetomidine in 0.5 ml saline. Primary outcomes were the incidence and intensity of shivering. Secondary outcomes were incidence of hypothermia (Temp < 36° C), sedation, the use of meperidine to control shivering and complications, bradycardia, nausea and vomiting. Results: C group showed statistically significant higher number of total patients who developed shivering (21), patients who developed grade IV shivering (20) and patients who needed meperidine (21) to treat shivering than M group (8,5,5) and D group (5,3,6) which were comparable to each other. Time needed to give meperidine after giving the block was similar in the three groups. Hypothermia didn’t occur in any patient in the three groups. The three groups were comparable regarding occurrence of nausea, vomiting, bradycardia & hypotension. All patients of C group, 32 patients in M group and 33 patients in D group had sedation score of 2. 3 patients in M group and 2 patients in D group had a sedation score of 3. and in of after in with The current study showed that both SA the of post
Use of Drones for Digitization and Monitoring the Built Cultural Heritage: Indoor and Outdoor

Digitizing is the way for a revolutionary approach in knowing, analyzing, continuous monitoring, and preserving the tangible immovable cultural heritage. The built cultural heritage requires the most performant means and techniques to acquire information indoor and outdoor. Drones are the best platforms for this purpose in terms of operating costs, data accuracy, and mission planning flexibility. In this chapter, we present a survey on the main applications of drones in the field of built cultural heritage analyzing the usability of this technology. Essential technical issues that are important for the operation and understanding of the use of drones in specific missions for the study of built heritage are also discussed.
Network Morphology

Network Morphology belongs to the family of inferential-realizational theoretical frameworks. This means that paradigms, more specifically the functions which construct them, play an important role. A major feature of Network Morphology is that it is based on defaults and allows for varying degrees of inheritance—from complete to partial—of paradigmatic structures. Network Morphology embraces computational implementation and has been applied to a range of typologically diverse languages. Computational fragments exist for languages belonging to a number of families, including Afro-Asiatic, Austronesian, Chukotko-Kamchatkan, Eskimo-Aleut, Gunwinyguan, Indo-European, Nakh-Daghestanian, Nilotic, and Nuclear Torricelli. It has also been used to model diachronic change.
Ethnic Discrimination and the Discourse of “Indigenization”: The Regional Regime, “Indigenous Majority” and Ethnic Minorities in Krasnodar Krai in Russia

To many in both the East and the West it seemed axiomatic that the collapse of the Soviet Union in 1991 was due to “nationality reasons,” which were viewed as a natural process in the last empire's decline. Then, during the democratic reform of a totalitarian state, ethnic minority rights were first spoken of, and the growth of national self-awareness appeared to be an integral part of society's liberalization. Time has since shown that liberal changes in the economy and in the political and social spheres are not always accompanied by the establishment of social justice; indeed, it has frequently been minorities who are among the most unfortunate and marginalized groups in society. Defending the rights of minorities and combating ethnic and racial discrimination remains one of the most relevant issues in practically all post-socialist countries of the former Soviet Union and Eastern and Central Europe.
Tracking Salmonella Enteritidis in the Genomics Era: Clade Definition Using a SNP-PCR Assay and Implications for Population Structure

Salmonella enterica serovar Enteritidis (or Salmonella Enteritidis, SE) is one of the oldest members of the genus Salmonella, based on the date of first description and has only gained prominence as a significant bacterial contaminant of food over the last three or four decades. Currently, SE is the most common Salmonella serovar causing foodborne illnesses. Control measures to alleviate human infections require that food isolates be characterized and this was until recently carried out using Pulsed-Field Gel Electrophoresis (PFGE) and phage typing as the main laboratory subtyping tools for use in demonstrating relatedness of isolates recovered from infected humans and the food source. The results provided by these analytical tools were presented with easy-to-understand and comprehensible nomenclature, however, the techniques were inherently poorly discriminatory, which is attributable to the clonality of SE. The tools have now given way to whole genome sequencing which provides a full and comprehensive genetic attributes of an organism and a very attractive and superior tool for defining an isolate and for inferring genetic relatedness among isolates. A comparative phylogenomic analysis of isolates of choice provides both a visual appreciation of relatedness as well as quantifiable estimates of genetic distance. Despite the considerable information provided by whole genome analysis and development of a phylogenetic tree, the approach does not lend itself to generating a useful nomenclature-based description of SE subtypes. To this end, a highly discriminatory, cost-effective, high throughput, validated single nucleotide based genotypic polymerase chain reaction assay (SNP-PCR) was developed focussing on 60 polymorphic loci. The procedure was used to identify 25 circulating clades of SE, the largest number so far described for this organism. The new subtyping test, which exploited whole genome sequencing data, displays the attributes of an ideal subtyping test: high discrimination, low cost, rapid, highly reproducible and epidemiological concordance. The procedure is useful for identifying the subtype designation of an isolate, for defining the population structure of the organism as well as for surveillance and outbreak detection.