Miracle-dz/UniFAR-FTUs huggingface dataset:
Occurrence and characterization of multiple novel genotypes of Mycobacterium immunogenum and Mycobacterium chelonae in metalworking ﬂuids

Rapidly growing mycobacteria colonize metalworking ﬂuids, leading to contamination of occupational environments and expo-sure-related respiratory illnesses in machine workers. Lately, it has been emphasized that these ﬂuids are colonizable by a single genotype of a rapidly growing mycobacterium species, Mycobacterium immunogenum . Here, we report on the genotypic diversity of mycobacteria in these ﬂuids, including isolation and characterization of multiple novel genotypes of two distinct species, Mycobacterium chelonae and M. immunogenum . Using agar culturing and Mycobacterium -speciﬁc PCR, 13 mycobacterial isolates were recovered from 100 geographically diverse in-use metalworking ﬂuid samples. Based on restriction fragment length polymorphism of PCR products, DNA sequencing ( hsp 65 gene segment), and phylogenetic analysis of 16S-23S rDNA internal transcribed spacer (ITS) sequences, six isolates were identiﬁed as M. immunogenum and seven as M. chelonae ; an additional isolate from metalworking ﬂuid diluent water was identiﬁed as M. diernhoferi . Genomic DNA macro-restriction fragment pattern analysis, using pulsed-ﬁeld gel electrophoresis with Xba I and Spe I restriction digestions, showed intraspecies variation among the isolates of M. immunogenum and M. chelonae. Visual and computer-assisted dendrogram analysis of the Xba I macro-restriction patterns revealed three novel genotypes of M. immunogenum and two of M. chelonae , whereas Spe I macro-restriction patterns revealed only two genotypes for each isolate. None of the identiﬁed genotypes matched the reportedly dominant one of M. immunogenum from metalworking ﬂuids. Both mycobacterial species are prevalent in metalworking ﬂuids and there is a considerable strain-level genetic diversity within them. (cid:1) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Effect of Signiﬁcant Two-Vessel Versus One-Vessel Coronary Artery Stenosis on Myocardial Contrast Defects Observed With Intermittent Harmonic Imaging After Intravenous Contrast Injection During Dobutamine Stress Echocardiography

Objectives. We sought to determine the effect of multivessel as opposed to single-vessel coronary artery stenosis on myocardial contrast defects observed with intermittent harmonic imaging and intravenous perﬂuorocarbon-exposed sonicated dextrose albumin contrast injection. Background. Intermittent harmonic imaging has permitted the detection of myocardial perfusion abnormalities with an intravenous ultrasound contrast agent. The effect of multivessel disease on inducibility of these perfusion abnormalities is unknown. Methods. In 10 dogs, intravenous injections of contrast agent were given at rest and during dobutamine stress echocardiography when a single coronary artery stenosis was present ( > (cid:50) 50% diameter by quantitative angiography) and again when a second stenosis (range 44% to 92% diameter) was present in the vessel supplying the adjacent perfusion bed. The peak myocardial contrast was visually and quantitatively assessed in the mid and lateral regions of the perfusion bed of the ﬁrst stenosis (original stenosis zone) in the presence of one- and two-vessel stenosis. Results. Peak myocardial contrast defects in both the mid and lateral segments of the original stenosis zone during dobutamine stress echocardiography was signiﬁcantly lower when two-vessel stenosis was present (p (cid:53) 0.015), especially in the lateral segment. The spatial extent of the perfusion defect in the original stenosis zone risk area increased signiﬁcantly when two-vessel stenosis was present, and correlated closely with actual risk area (r (cid:53) 0.99). Previous total occlusion followed by reperfusion of the vessel supplying the original stenosis zone signiﬁcantly increased the amount of collateral activity between perfusion beds. spatial extent of inducible ischemia within the risk area of single-vessel stenosis. Restor-ing blood ﬂow to one perfusion bed reduces the extent of a perfusion abnormality that can be induced in an adjacent stenosed bed.
Impact of Endocytosis Mechanisms for the Receptors Targeted by the Currently Approved ADCs—A Necessity for Future ADC Research and Development

: Biologically-based therapies increasingly rely on the endocytic cycle of internalization and exocytosis of target receptors for cancer therapies. However, receptor trafﬁcking pathways (endosomal sorting (recycling, lysosome localization) and lateral membrane movement) are often dysfunctional in cancer. Antibody-drug conjugates (ADCs) have revitalized the concept of targeted chemotherapy by coupling inhibitory antibodies to cytotoxic payloads. Signiﬁcant advances in ADC technology and format, and target biology have hastened the FDA approval of nine ADCs (four since 2019). Although the links between aberrant endocytic machinery and cancer are emerging, the impact of dysregulated internalization processes of ADC targets and response rates or resistance have not been well studied. This is despite the reliance on ADC uptake and trafﬁcking to lysosomes for linker cleavage and payload release. In this review, we describe what is known about all the target antigens for the currently approved ADCs. Speciﬁcally, internalization efﬁciency and relevant intracellular sorting activities are described for each receptor under normal processes, and when complexed to an ADC. In addition, we discuss aberrant endocytic processes that have been directly linked to preclinical ADC resistance mechanisms. The implications of endocytosis in regard to therapeutic effectiveness in the clinic are also described. Unexpectedly, information on endocytosis is scarce (absent for two receptors). Moreover, much of what is known about endocytosis is not in the context of receptor-ADC/antibody complexes. This review provides a deeper understanding of the pertinent principles of receptor endocytosis for the currently approved ADCs. or refractory multiple myeloma after at least four prior therapies including an anti-CD38 mAb, a proteasome inhibitor, and an immunomodulatory agent to mAb/ADC binding and endocytosis and intracellular trafﬁcking.
Applying “SEVALERPS” a Systematic Evaluation Method for ERP selection in a Public Administration’s Case Study

— Enterprise Resource Planning (ERP) systems’ evaluation is an important activity to undertake in any ERP acquisition project. Empirical research has shown that selecting an inappropriate ERP system is a major reason for its implementation failure. Actually, inadequately selected ERP systems may affect companies’ market share and increase implementation time, effort and cost relating to these solutions. These negative impacts could even jeopardize the very existence of large organizations. A review of relevant literature has identified a need for methodologies, methods and approaches which could assist organizations in the risky, time consuming and complex activity of ERP selection. In this regard, we have developed in our previous works a systematic method, called SEVALERPS ( S ystematic EVAL uation for ERP Systems), to help organizations to choose among the available ERP solutions, the ones that could best fit their requirements. This paper aims to validate the SEVALERPS method by applying it in a public administration’s case study. This case study helps to illustrate the method and to discuss its added value.
Introductory Chapter: Meat Science and Human Nutrition Introductory Chapter: Meat Science and Human Nutrition

Meat holds a pivotal position among most of the communities. Meat is viewed as a sustenance of high nutritive incentive as its protein has 70% of biological availability in the body, and therefore it is frequently seen as the main food while planning meals. It is comparatively a good source of metabolically active iron and furthermore enhances its absorption from other food sources, its amino acid composition supplements the nutrition of many plant foods, and it is a concerted supply of vitamin B complex, including vitamin B12, which is not present in plant foods. Therefore, meat and meat products are preferred to fulfill protein requirements [1].
Unusual Presentation of Postprandial Sinus Tachycardia in Pregnancy

This report describes the clinical presentation and systematic workup of a 25-year-old primigravid woman presenting at 34 weeks gestation with symptoms of postprandial sinus tachycardia. Differentials included pregnancy-related inappropriate sinus tachycardia (PRIST) and swallowing-induced atrial tachycardia. PRIST has favorable outcomes for both the mother and baby, although the symptoms can be distressing and lead to the overutilization of healthcare resources. The literature describes symptom onset as sporadic with no definitive triggers, often occurring in the later trimesters of pregnancy. Swallowing-induced atrial tachycardia, which fits with postprandial symptoms seen in this patient, is a distinct entity most commonly diagnosed in middle-aged men. Management included psychosocial support and pharmacologic treatment with labetalol. The patient’s course was benign with an uncomplicated delivery at 38 weeks, complete resolution of symptoms in the postpartum period, and successful discontinuation of pharmacologic management with no relapses. Current literature on postprandial sinus tachycardia in pregnant women is limited, and the best treatment method is unknown. This report extends current knowledge on pregnancy-related postprandial sinus tachycardia and provides a framework for advances in the management of this clinical presentation.
S -( 1 )-3-Isobutylgaba and Its Stereoisomer Reduces the Amount of Inflammation and Hyperalgesia in an Acute Arthritis Model in the Rat 1

The present study investigated whether spinal administration of S -( 1 )-3-isobutylgaba ( S -( 1 )-3-IBG) or its stereoisomer, R -( 2 )-3-isobutylgaba ( R -( 2 )-3-IBG), are effective in reducing the hyperalgesia and swelling observed after injection of kaolin and carrageenan into the knee joint of the rat. The effects of pretreatment and post-treatment of S -( 1 )-3-IBG, R -( 2 )-3-IBG and artificial cerebrospinal fluid (aCSF) on the swelling, pain-related behavior scores and the heat hyperalgesia induced by knee joint inflammation were compared. Infusion of either S -( 1 )-3-IBG or R -( 2 )-3-IBG through a microdialysis fiber, implanted in the dorsal horn of the spinal cord, for 1.5 h before injection of kaolin and carrageenan resulted in a 20 to 30% reduction in joint swelling compared with aCSF-treated controls, and pre- vented the development of heat hyperalgesia and spontaneous pain. In contrast, infusion of either stereoisomer after the de- velopment of inflammation reduced the hyperalgesia but did not reduce the amount of joint swelling compared with aCSF- treated animals. In summary, S -( 1 )-3-IBG and R -( 2 )-3-IBG are effective antihyperalgesic agents when administered both be- fore and after joint inflammation. In addition, if administered before injection of kaolin and carrageenan into the knee joint this drug can attenuate joint inflammation. Both the antihyperalgesic and anti-inflammatory properties of this drug probably are mediated through a central neurogenic
HORIZONS Photoheterotrophy in marine prokaryotes

Despite considerable advances in the understanding of the various microbial photoheterotrophic mechanisms, the role of solar radiation in the metabolism of bacterioplankton in the ocean is difﬁcult to assess. It is already apparent that rates of CO 2 ﬁxation by prokaryotic cells may be only a part of the picture. Photophosphorylation is difﬁcult to differentiate from respiratory phosphorylation and other types of ATP synthesis. Solar energy could by-pass ATP synthesis, instead being used to generate a proton-motive force, which in turn could be directly used for cell motility or even for importing molecules into cells. In addition, photoheterotrophic prokaryotes could actively regulate intake and use of solar energy for different metabolic functions depending on the energetic demands of the cell. The factors listed above hence require consideration when solar energy input into metabolism of oceanic photoheterotrophic prokaryotes is experimentally quantiﬁed and numerically modelled.
On the Sraffa-Leontief Model

In this paper we consider the matrix forms of the Sraffa-Leontief income distribution model introduced by Steenge (1995, 1997). We will explore the equivalence between these matrix forms and the set of simpler models, including the Sraffian condition of linear relations between the rate of profits r and wage rate w*. Further, we will evaluate the condition that the price vector p and the commodities vector x are the left-hand and the right-hand eigenvectors of the matrix A of direct inputs and that these vectors are the fixed points of the Sraffian standard commodities-standard prices matrix. We will then explore links between the Sraffa-Leontief system and the multiplier product matrix (MPM) for the matrix A to consider new insights generated through visualization with the help an artificial economic landscape. Furthermore, the connections between MPM and the Sraffian standard commodities-standard prices matrix and their minimal information properties are proven.
Maternal obesity and its effect on labour duration in nulliparous women

Background: Obesity is increasing among primipara women. We aimed to describe the association between body mass index (BMI) during early-pregnancy and duration of labour in nulliparous women. Methods: Retrospective observational cohort study of 1885 nulliparous women with a single cephalic presentation from 37 0/7 to 42 6/7 weeks of completed gestation and spontaneous or induced labour at Nordsjællands Hospital, University of Copenhagen, Denmark, in 2011 and 2012. Total duration of labour and the first and second stages of labour were compared between early-pregnancy normal-weight (BMI <25 kg/m 2 ), overweight (BMI 25 – 29.9 kg/m 2 ), and obese (BMI ≥ 30 kg/m 2 ) women. Proportional hazards and multiple logistic regression models were applied. Results: Early pregnancy BMI classified 1246 (66.1%) women as normal weight, 350 (18.6%) as overweight and 203 (10.8%) as obese. No difference in the duration of total or first stage of active labour was found for overweight (adjusted HR = 1.01, 95% CI 0.88 – 1.16) or obese (adjusted HR = 1.07, 95% CI 0.90 – 1.28) compared to normal weight women. Median active labour duration was 5.83 h for normal weight, 6.08 h for overweight and 5.90 h for obese women. The risk of caesarean delivery increased significantly for overweight and obese compared to normal weight women (odds ratios (OR) 1.62; 95%CI 1.18 – 2.22 and 1.76; 95%CI 1.20 – 2.58, respectively). Caesarean deliveries were performed earlier in labour in obese than normal-weight women (HR = 1.80, 95%CI – 2.54). Conclusion: BMI had no significant effect on total duration of active labour. Risk of caesarean delivery increased with BMI.
Explicit characterization of decentralized coprime factors (cid:2)

This paper is concerned with the parametrization of all the decentralized stabilizing controllers. The auxiliary diagonal system, which is deﬁned by the diagonal elements of Bezout factors, plays important roles in the parametrization of decentralized controllers. This paper gives an explicit characterization of the auxiliary system. Abstract This paper is concerned with decentralized control problems. There are two typical procedures to design decentralized con- trollers, ‘independent’ and ‘sequential’ design procedures. As the concepts and the techniques in these two approaches are too diﬀerent from each other, there have been no attempts to unify these approaches. This paper proposes an iterative independent design procedure for decentralized control systems, which is a uniﬁed approach to these conventional approaches.
Seventy-five years of global analysis around the forced pendulum equation ∗

We survey the recent progress made in the study of harmonic, subharmonic and other solutions of the forced pendulum equation u ′′ + cu ′ + asin u = h(t) when the forcing term h is periodic, almost periodic or bounded. The results depend upon various methods of nonlinear functional analysis, critical point theory and dynamical systems.
Fatty Acid Biosynthesis in Mitochondria of Grasses: Malonyl-Coenzyme A Is Generated by a Mitochondrial-Localized Acetyl-Coenzyme A Carboxylase 1

We present biochemical evidence for the occurrence of a 250-kD multifunctional acetyl-coenzyme A carboxylase in barley ( Hordeum vulgare ) mitochondria. Organelles from 6-d-old barley seedlings were purified by differential centrifugation and Percoll density gradient centrifugation. Upon analysis by two-dimensional Blue-native (BN)/SDS-PAGE, an abundant 250-kD protein can be visualized, which runs at 500 kD on the native gel dimension. A similar 500-kD complex is present in etioplasts from barley. The mitochondrial 250-kD protein is biotinylated as indicated by specific reaction with an antibody directed against biotin. Peptide sequence analysis by electrospray ionization tandem mass spectrometry of the 250-kD proteins from both organellar fractions revealed amino acid sequences that are 100% identical to plastidic acetyl-coenzyme A carboxylase from wheat ( Triticum aestivum ). The 500-kD complex was also detected in wheat mitochondria, but is absent in mitochondrial fractions from Arabidopsis. Specific acetyl-coenzyme A carboxylation activity in barley mitochondria is higher than in etioplasts, suggesting an important role of mitochondria in fatty acid biosynthesis. Functional implications are discussed.
Survey on Detection of Gray Hole and Black Hole in Mobile Ad-Hoc Network

— obile Ad-hoc N/w is a network in which different mobile nodes or devices are present and they wants to communicate with each other using shared wireless medium. In this network all nodes are having their own communication range. During communication each nodes acts as router to forward packets to another node. In MANET there is no centralised administration. So, there is high possibility of different types of attacks on network. Gray Hole attack and Black hole attack are such attacks. In this Gray hole and Black Hole are the malicious nodes which forward wrong information about communication route. So for good communication it is very important to keep communication rout free from these type of attacks many people have worked on this. In this paper different ideas of different peoples are provided to detect Gray Hole and Black Hole attacks.
User Density Estimation System using High Frequencies in a Specific Closed Space

—In this paper, we propose a user density estimation system using high frequencies and the microphone of a smart device in a specific closed space. High frequencies are sent to the closed space by the server speaker of the proposed system, and smart devices located in the space detect the high frequencies. The smart devices detecting the high frequencies send a message to the server system, and the system counts the smart devices that detected the high frequencies in the space. We tested user density with the proposed system, using 10 smart devices to evaluate performance. According to the test results, the proposed system showed 95% accuracy. The system can estimate exact user density in a specific closed space, and it can be a useful technology for protecting people’s safety and measuring space use in indoor spaces.
Development and Characterisation of a Nanocomposite Membrane Using Polyethersulphone and Graphene Oxide-Magnetite Nanoparticles for Rejection of Chromium Hexavalent Ions from Tannery Wastewater

Polyethersulphone (PES) polymer blended with Graphene Oxide (GO) and magnetite ( nano-composite was synthesized using an immersion precipitation process for the filtration of chromium hexavalent ions from leather processing waste water. PES is a synthetic polymer with good chemical resistance, wide pH range (2-13) as well as good mechanical and thermal properties. However, it is hydrophobic and prone to fouling hence its limitations in aqueous separation processes. In order to improve hydrophilicity, porosity, permeability and strength properties of the polymeric membrane, GO was synthesized from coal via a modified Hummers Method and then blended with anhydrous ferrous chloride to form Graphene oxide/ magnetite () hybrid nano-particles. To assess the performance of the membrane pure water flux, Equilibrium Water Content (EWC) and tensile strength test was measured. Structural analysis involved porosity measurement. The filtration efficiency of the membranes was found by testing the waste water for chromium ion concentration before and after filtration. It was established that both the physical properties and chromium ion rejection improved with increase in polymer concentration and addition of hybrid nano-particles.
The use of yellow fluorescent hybrids to indicate mating in Trypanosoma Parasites

Background: Trypanosoma brucei undergoes genetic exchange in its insect vector, the tsetse fly, by an unknown mechanism. The difficulties of working with this experimental system of genetic exchange have hampered investigation, particularly because the trypanosome life cycle stages involved cannot be cultured in vitro and therefore must be examined in the insect. Searching for small numbers of hybrid trypanosomes directly in the fly has become possible through the incorporation of fluorescent reporter genes, and we have previously carried out a successful cross using a reporter-repressor strategy. However, we could not be certain that all fluorescent trypanosomes observed in that cross were hybrids, due to mutations of the repressor leading to spontaneous fluorescence, and we have therefore developed an alternative strategy. Results: To visualize the production of hybrids in the fly, parental trypanosome clones were transfected with a gene encoding Green Fluorescent Protein (GFP) or Red Fluorescent Protein (RFP). Co-infection of flies with red and green fluorescent parental trypanosomes produced yellow fluorescent hybrids, which were easily visualized in the fly salivary glands. Yellow trypanosomes were not seen in midgut or proventricular samples and first appeared in the glands as epimastigotes as early as 13 days after fly infection. Cloned progeny originating from individual salivary glands had yellow, red, green or no fluorescence and were confirmed as hybrids by microsatellite, molecular karyotype and kinetoplast
An Efficacious Hardware Enactments of Canny Edge type Diagnosis Algorithm

— The edge location is one of the key systems in most picture handling applications. The shrewd edge location is turned out to be ready for fundamentally to beat at existing edge identification procedures because of its unrivaled execution. Sadly, the execution of the schema progressively is computationally not acceptable and high equipment taken a toll with expanded idleness. The suggested vigilant edge identification calculation utilizes estimation techniques to supplant the mind boggling operations; the pipelining is utilized to lessen the dormancy. So in this outline, the picture is converted to 512 x 512 pixel esteems utilizing MATLAB. These pixel esteems are in Binary arrangement. It produces aggregate of 262144 pixel esteems. These double esteems are perused in Verilog utilizing Xilinx and it creates the edge distinguished yield esteems. And at long last edge identified yield esteems are perused in the aforementioned and amended over back to edge recognized picture. At long last, this estimation is actualized on Xilinx. At the point, when contrasted and the past equipment engineering for vigilant edge location, the suggested design requires less equipment expenses and takes onems to identify the edges of 512x512