in Domestic Animals

Vitriﬁ cation in straws conserves motility features better than spheres in donkey sperm Homologous seminal plasma (SP) is reported to have preventive and cryodamage reverse effects on cryopreserving ram spermatozoa (CS) (Barrios et al. 2000, Biol Reprod 63:1531–7). The objectives of this research were to detect alterations in CS viability and motility assets following supplementation of SP, and to determine the time of their occurrence. Homogenous quality ejaculates of Ovchepolean Pramenka rams (N = 10) were pooled and allocated in four groups: K1, E1, K2, and E2, each with equal sample size (n = 25). K1, K2 and E2 were extended with control (CE- soybean based), whereas E1 was extended with experimental extender (EE- control+20% SP) prior deep- freezing. Post- thawing, samples were placed at 37°C; K2 and E2 were diluted in 1:1 ratio with CE and EE, respectively, and analyzed by CASA for a sequence of parameters (viable cells- Viab, total motility- tMot, progressive motility- pMot, fast velocity- FVel) in two- time periods of incubation: 0- h and 3- h. Wilks and least significant difference post- hock tests revealed no significant difference between K1 and K2 during incubation (p > 0.05). K2 values for Viab and tMot were no different to E2 for 0- h (p > 0.05), whereas following 3- h incubation, E2 indicated significantly higher values in all parameters (p < 0.001). E1 indicated significantly higher values for Viab and tMot than E2 on 0- h (p < 0.01), but following 3- h incubation, no significant differences were found (p > 0.05). Results are in-dicative that SP affects CS viability and motility when it’s utilized as additive in extenders, and its effects are occurring with significantly higher extent following incubation for 3 h at 37°C. to the of different on the sperm of different concentrations of sucrose (0.1 M (Group II), (Group III) and M (Group IV)) proportion v/v with HTF- BSA Before vitrification and after standard semen plasma membrane integrity, sperm mitochondrial membrane potential, apoptosis, sperm chro-matin and Motile Sperm Organelle Morphology Examination (MSOME) determined. Results showed a significant decrease of progressive motility (PR) of sperm after addition 0.5 M sucrose solutions, it is 54.6% in control vs. 28.6%, p ≤ 0.01). Fast addition of sucrose solu tions causes a greater decrease in sperm PR than slow addition. After thawing spermatozoa of group II showed the highest plasma membrane integrity (20 ± 0.7%) and total acrosome integrity (70 ± 1.0%) in comparison with 0.1 M (p ≤ 0.05) and 0.5 M (p ≤ 0.01). There was no differ ence in spermatozoa DNA fragmentation rate among the experimental groups. Sucrose increased early and late apoptotic effects. There was no difference between the experimental groups in MSOME results. In con-clusion the 0.25 M sucrose solution seems to be the most appropriate concentration for the vitrification of goat sperm. the level of BCB+ oocytes clusters of [76% (60/79) vs. 90% (54/60), p The mechanism of the influence of HDSn other organelles calcium in egg shell that provides the hardness of egg shell of hatching eggs, by 9.4%. The incubation wastes regarding blood ring in the experimental group were not identified, and day- long chicks had more intensive pigmentation of floccus. The manure of the hens from the experimental group was more solid which resulted in reduction of wetness of bedding and impurity of hatching eggs. Improvement of reproductive potential of the laying hens from parent broiler flock is caused by lipolytic, antibacterial, hepatoprotective, and anti- inflammatory characteristics of supplements based on triterpene spirits. The aim of this study was to evaluate the influence of repeated low doses of buserelin on LH and FSH secretion in anoestrous cows with ovarian follicles smaller than 5 mm and without a cor-pus luteum. Five cows from experimental group (EG) received 0.0042 mg (1 ml) of buserelin (Receptal, MSD, Poland) i.m. and 5 control cows (CG) 1 ml of saline (i.m.) 5 times 24 h apart. After every injection blood samples were collected every 20 min for 6 h, centrifuged serum separated and stored in a freezer at - 70°C. FSH and LH concentrations were measured by radioimmunoassay. Ultrasound examination of the ovaries was performed every day. After treatment cows were controlled by ultrasonography once a week for 5 weeks. The average FSH concentration in EG cows was 1.66 times higher than in CG cows (0.37 ng/ml (0.18–0.52) vs. 0.23