Miracle-dz/UniFAR-FTUs huggingface dataset:
Aboveground Herbivory Influences Belowground Defense Responses in Maize

The European corn borer (ECB; Ostrinia nubilalis) is an economically damaging insect pest of maize (Zea mays L.), an important cereal crop widely grown globally. Among inbred lines, the maize genotype Mp708 has shown resistance to diverse herbivorous insects, although several aspects of the defense mechanisms of Mp708 plants are yet to be explored. Here, the changes in root physiology arising from short-term feeding by ECB on the shoot tissues of Mp708 plants was evaluated directly using transcriptomics, and indirectly by monitoring changes in growth of western corn rootworm (WCR; Diabrotica virgifera virgifera) larvae. Mp708 defense responses negatively impacted both ECB and WCR larval weights, providing evidence for changes in root physiology in response to ECB feeding on shoot tissues. There was a significant downregulation of genes in the root tissues following short-term ECB feeding, including genes needed for direct defense (e.g., proteinase inhibitors and chitinases). Our transcriptomic analysis also revealed specific regulation of the genes involved in hormonal and metabolite pathways in the roots of Mp708 plants subjected to ECB herbivory. These data provide support for the long-distance signaling-mediated defense in Mp708 plants and suggest that altered metabolite profiles of roots in response to ECB feeding of shoots likely negatively impacted WCR growth.
Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation

Pasteurella multocida is one of the primary pathogens of bovine respiratory disease (BRD), and causes huge losses in the cattle industry. The Pm3 strain was a natural isolate, which is a strong form of pathogen and is sensitive to fluoroquinolones antibiotics. A high fluoroquinolone resistant strain, Pm64 (MIC = 64 μg/mL), was formed after continuous induction with subinhibitory concentration (1/2 MIC) of enrofloxacin, with the enhanced growth characteristics and large attenuation of pathogenicity in mice. This study reports the whole genome sequence and the transcription profile by RNA-Seq of strain Pm3/Pm64. The results showed an ineffective difference between the two strains at the genome level. However, 32 genes could be recognized in the gene islands (GIs) of Pm64, in which 24 genes were added and 8 genes were lost. Those genes are involved in DNA binding, trehalose metabolism, material transportation, capsule synthesis, prophage, amino acid metabolism, and other functions. In Pm3 strain, 558 up-regulated and 568 down-regulated genes were found compared to Pm64 strain, from which 20 virulence factor-related differentially expressed genes (DEGs) were screened. Mainly differentially transcribed genes were associated with capsular polysaccharide (CPS), lipopolysaccharide (LPS), lipooligosaccharide (LOS). Iron utilization, and biofilm composition. We speculated that the main mechanism of virulence attenuation after the formation of resistance of Pm64 comes from the change of the expression profile of these genes. This report elucidated the toxicity targets of P. multocida serogroup A which provide fundamental information toward the understanding of the pathogenic mechanism and to decreasing antimicrobial drugs resistance.
Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332—A Preliminary Study

Human amniotic cells (hAC) exhibit characteristics of undifferentiated cells and immunomodulatory properties. Recognition of the relationship between amniotic cells and components of the extracellular matrix is an important condition for their ex vivo preparation and further successful clinical application in regenerative medicine and transplantology. Laminin 332 (LN-332), as a natural component of the basement membrane of amniotic epithelial cells and a ligand for integrin receptors, may strongly influence the phenotype and fate of amniotic cells. We investigated the impact of recombinant LN-332 on hAC viability and expression of markers for pluripotency, early differentiation, adhesion, and immunomodulatory properties. During 14 days of culture, hAC were quantified and qualified by light microscopy, immunohistochemistry, immunocytochemistry, and flow cytometry. Gene expression was assessed with real-time polymerase chain reaction (RT-PCR) arrays and compared with differentiated cells originated from the three germ layers. LN-332 caused an over 2-fold increase in the total number of hAC, accompanied by a 75% reduction of SSEA-4-positive cells and an increase in HLA-ABC-positive cells. In particular, we observed that the presence of laminin 332 in the medium of a short-time culture modifies the effect of culture duration on hAC, enhancing time-dependent inhibition of expression of certain genes, including pluripotency and differentiation markers, laminin 332 subunits (which may be part of self-regulation of LN-332 synthesis by amniotic cells), and integrins. The changes observed in hAC were more distinct with respect to differentiated mesenchymal cells, resulting in more comparable phenotypes than those represented by differentiated endo- and ectodermal cells. We concluded that laminin 332 present in the culture medium influences to a certain extent proliferation, adhesion, and differentiation of amniotic cells in culture.
GH Responsiveness in Children With Noonan Syndrome Compared to Turner Syndrome

Background Despite different genetic background, Noonan syndrome (NS) shares similar phenotype features to Turner syndrome (TS) such as short stature, webbed neck and congenital heart defects. TS is an entity with decreased growth hormone (GH) responsiveness. Whether this is found in NS is debated. Methods Data were retrieved from combined intervention studies including 25 children diagnosed with NS, 40 diagnosed with TS, and 45 control children (all prepubertal). NS-children and TS-girls were rhGH treated after investigation of the GH/IGFI-axis. GH was measured with poly- and monoclonal antibodies; 24hGH-profile pattern analysed by PULSAR. The NS-children were randomly assigned to Norditropin® 33 or 66 μg/kg/day, and TS-girls were consecutively treated with Genotropin® 33 or 66 μg/kg/day. Results Higher PULSAR-estimates of 24h-profiles were found in both NS-children and TS-girls compared to controls: Polyclonal GHmax24h-profile (Mean ± SD) was higher in both groups (44 ± 23mU/L, p<0.01 in NS; 51 ± 47, p<0.001 in TS; compared to 30 ± 23 mU/L in controls) as was GH-baseline (1.4 ± 0.6 mU/L in NS; 2.4 ± 2.4 mU/L in TS, p<0.01 for both, compared to 1.1 ± 1.2 mU/L in controls). Pre-treatment IGFISDS was 2.2 lower in NS-children (-1.7 ± 1.3) compared to TS-girls (0.6 ± 1.8, p<0.0001). GHmax, IGFI/IGFBP3-ratioSDS, and chronological age at start of GH accounted for 59% of the variance in first-year growth response in NS. Conclusion Both prepubertal NS-children and TS-girls had a high GH secretion, but low IGFI/IGFBP3 levels only in NS-children. Both groups presented a broad individual response. NS-children showed higher response in IGFI and growth, pointing to higher responsiveness to GH treatment than TS-girls.
Impulsivity and Difficulties in Emotional Regulation as Predictors of Binge-Watching Behaviours

Recently, the question about the potentially problematic characteristics of binge-watching behaviours has been raised in the contemporary literature. Binge-watching is a highly popular behaviour that involves watching multiple episodes of TV series in one sitting. Studies show that binge-watching can be both an entertaining, but also a potentially problematic, behaviour. Therefore, this research aimed to answer the question about how impulsivity, difficulties in emotional regulation, and one's motivations around why they want to watch a TV series predict problematic binge-watching among a group of Polish young adults. The research group consisted of 645 participants. The following tools were then used to measure the study variables: the Impulsive Behaviour Scale, the Difficulties in Emotion Regulation Scale, the Viewing Motivation Scale, and the Questionnaire of Excessive Binge-watching. Furthermore, a regression analysis was performed on the responses to each measure in order to answer our research questions. Our results show that a lack of premeditation, impulse control difficulties, and having an escapist motivation are all significant predictors of problematic binge-watching behaviours. Furthermore, one's motivations around dealing with loneliness, their motivations around how to best spend their free time, as well as their informative and entertaining motivations were also found to be significant predictors of problematic binge-watching behaviours.
A Diagnostic Survey of Aborted Equine Fetuses and Stillborn Premature Foals in Denmark

Background: Loss of pregnancy in mares can have many different causes, including both infectious and non-infectious conditions. Extrapolation of findings from other studies is often uncertain as the significance of each cause varies across regions. Causes of pregnancy loss in mares have never been thoroughly studied in Denmark, so a prospective cross-sectional cohort study targeting the entire Danish population of pregnant mares was performed over a period of 13 months to obtain knowledge of the significance of individual causes. Fifty aborted or prematurely delivered stillborn fetuses were submitted for necropsy and examined by a panel of diagnostic laboratory methods. Results: Overall, a cause of fetal loss was established for 72% of the examined cases. Most cases (62%) were lost due to a non-infectious cause, of which obstruction of the feto-placental blood circulation due to severe torsion of the umbilical cord was most prevalent. Pregnancy loss due to a variety of opportunistic bacteria, including bacteria not previously associated with abortion in mares, accounted for 12%, while equid alphaherpesvirus (EHV) type 1 was the cause of pregnancy loss in 8% of the cases. EHV type 4 and Chlamydiaceae species were identified in some cases, but not regarded as the cause of fetal loss. Conclusion: Umbilical cord torsion was found to be the most prevalent cause of fetal loss in Danish mares, while infectious causes such as EHV type 1 and streptococci only accounted for a minor proportion of the losses. The study highlights the need for defined criteria for establishing an abortion diagnosis in mares, particularly in relation to EHV types 1 and 4.
Culture Enrichment Combined With Long-Read Sequencing Facilitates Genomic Understanding of Hadal Sediment Microbes

Culture enrichment was developed to discover the uncharted microbial species in the environmental microbiota. Yet this strategy has not been widely used to study microbes of deep-sea environments. Here, we report the cultivation and metagenomic analysis of oceanic sediment microbiota collected from 6,477 m deep in the Mariana Trench. The sediment samples were cultured anaerobically in the laboratory for 4 months, before being subjected to full-length 16S rRNA gene sequencing using the PacBio technique and metagenome sequencing using both the Illumina and Oxford Nanopore techniques. The 16S rRNA gene analyses revealed 437 operational taxonomic units specific to the cultured microbes, despite the lower diversity of the cultured microbiota in comparison with the original. Metagenome analyses revealed the prevalence of functions related to respiration, energy production, and stress response in the cultured microbes, suggesting these functions may contribute to microbial proliferation under laboratory conditions. Binning of the assembled metagenome contigs of the cultured microbiota generated four nearly complete genomes affiliated to yet unclassified species under the genera Alcanivorax, Idiomarina, Sulfitobacter, and Erythrobacter. Excepting Alcanivorax, the other three genera were almost undetectable in the original samples and largely enriched in the cultured samples. The four genomes possessed a variety of genes for carbohydrate utilization and nitrite reduction, pointing to an ability to respire diverse carbon sources using nitrite as the final electron acceptor. Taken together, the findings suggest that a combination of culture enrichment and long-read sequencing is an ideal way to mine novel microbial species in the hadal environment, particularly species that are rare in their native environmental niches, and thus expand our understanding of the hadal microbial diversity and function.
Studies and Application of Sialylated Milk Components on Regulating Neonatal Gut Microbiota and Health

Breast milk is rich in sialic acids (SA), which are commonly combined with milk oligosaccharides and glycoconjugates. As a functional nutrient component, SA-containing milk components have received increasing attention in recent years. Sialylated human milk oligosaccharides (HMOs) have been demonstrated to promote the growth and metabolism of beneficial gut microbiota in infants, bringing positive outcomes to intestinal health and immune function. They also exhibit antiviral and bacteriostatic activities in the intestinal mucosa of new-borns, thereby inhibiting the adhesion of pathogens to host cells. These properties play a pivotal role in regulating the intestinal microbial ecosystem and preventing the occurrence of neonatal inflammatory diseases. In addition, some recent studies also support the promoting effects of sialylated HMOs on neonatal bone and brain development. In addition to HMOs, sialylated glycoproteins and glycolipids are abundant in milk, and are also critical to neonatal health. This article reviews the current research progress in the regulation of sialylated milk oligosaccharides and glycoconjugates on neonatal gut microbiota and health.
DESCRIPTION OF A NOZZLE TEST FACILITY.

Abstract : The mechanical properties of a facility designed to study the characteristics of hybrid nozzles are described. Castigliano's method is used to analyze the deflection and load relationship of the rigid but elastic system. Experiments substantiate the analysis and confirm the capability for measuring nozzle thrust with a high degree of precision. The second part of the report describes tests with a family of axisymmetric nozzles. Also included is a detailed statement of calibration and testing procedures and an assessment of the total system accuracy. (Author)
A new method to quantify harmony of the horse–rider system in dressage

As in many other sports, e.g. gymnastics, judging dressage riding is problematic because the score is subjective. The aim of this study was to find a suitable method to support education of dressage judges and training of riders with a measurable criterion for riding harmony in the trot. We analysed the consistency of motion pattern 40 different rider–horse systems in trot (20 horses and 2 riders). A high-speed (120 Hz) 3D video system for motion analysis was used to track 20 markers taped to the horse and the rider. The angle between the line connecting the rider’s head to the rider’s back and that between the rider’s back to the horse’s head was calculated. Angular velocity and angular acceleration were derived. The lengths of the resulting vectors (LV) in the phase space were computed. Riding harmony was defined in terms of the average deviation of LV in the phase space. The results of our study showed the professional rider–horse system had a significantly (P < 0.05) lower average deviation of LV (11.5% ± 1.4) than the recreational rider–horse system (13% ± 2.8). Thus, the professional rider–horse system had a motion pattern that was more consistent than the recreational rider–horse system and this was correlated to the average dressage scores, which were significantly (P < 0.001) higher for the professional rider (mean score ± SD, 7.3 ± 2.7) than those for the recreational rider (4.1 ± 3.0). As motion pattern consistency is one of the main characteristics of riding harmony, the results of these measurements can be used for education of dressage judges and riders.
Estimating business value of academic research outcomes: towards a multi-dimensional approach

As academic entrepreneurship develops, questions of business valuation of research become more important. Therefore, there is a need to develop methods for value assessment of academic research. Conventional business valuation models for technology are usually financially oriented and will only disclose its economic value. However, this under specifies the value of university-industry transactions. The value of an invention not only depends on economic value but also on strategic, cultural or social value added to the applicant of the invention: usually a company. We introduce gross business value, being a function over time of these four dimensions of value and propose to position this gross business value of an academic technological invention within a three-dimensional model, with the research value creation process on the X-axis, the type of research on the Y-axis and its contribution to the applicant's four types of capitals at the Z-axis.
Novel CMOS current Schmitt trigger

A novel CMOS current Schmitt trigger based on current mirrors is presented, using only seven MOS transistors. The significant feature of the circuit is that one threshold current is controlled by a bias current and the other by an MOS transistor dimension, depending on the circuit implementation. A design example and the simulation results are given.
Smooth and energy saving gait planning for humanoid robot using geodesics

A novel gait planning method using geodesics for humanoid robot is given in this paper. Both the linear inverted pendulum model and the exact Single Support Phase SSP are studied in our energy optimal gait planning based on geodesics. The kinetic energy of a 2-dimension linear inverted pendulum is obtained at first. We regard the kinetic energy as the Riemannian metric and the geodesic on this metric is studied and this is the shortest line between two points on the Riemannian surface. This geodesic is the optimal kinetic energy gait for the COG because the kinetic energy along geodesic is invariant according to the geometric property of geodesics and the walking is smooth and energy saving. Then the walking in Single Support Phase is studied and the energy optimal gait for the swing leg is obtained using our geodesics method. Finally, experiments using state-of-the-art method and using our geodesics optimization method are carried out respectively and the corresponding currents of the joint motors are recorded. With the currents comparing results, the feasibility of this new gait planning method is verified.
Lean production: literature review and trends

The purpose of this paper is to perform the analysis of literature review of lean production (LP). The analysis involved studying 546 research articles published from 1988 to 2011 in selected 24 operations research journals. The articles are classified by: time distribution of articles, research methodology, research stream, authorship patterns, sector-wise focus, popular elements in lean literature, focus of the articles on lean waste, and implementation status and performance measurement of various existing frameworks/models. Then, the suggestions for the future scope of research possibilities and development are identified. The study provides a taxonomical and integrated review of articles, puts up perspective into the conceptualisation and various critical parameters for research. The findings include: increase in empirical approach research articles, need of applying lean principles in the field of product development and enterprise level areas, need of more interregional research collaborations, need of lean elements as group instead of individual element, need of avoiding seven lean wastes instead of specific waste and lack of testing and validation of the proposed frameworks/models by researchers. The study results shall help researchers, academicians and professionals to focus on the growth, pertinence and research developments in the LP system field.
Robust Portfolio Optimization with Transaction Cost for Risky and Risk-Free Assets Coexisting

A robust mean-variance portfolio selection model with transaction cost is presented for the case that both risky and risk-free assets exist in the market and expected returns of assets are uncertain and belong to a convex polyhedron. The model helps investors to identify such portfolios that expectations of investors are ensured even if the worst case in the expected returns of assets occurs. Analytical expression of the optimal portfolio determined by the proposed model is derived based on the Lagrange method for constrained optimization. Empirical analysis with three real stocks is performed to give the efficient frontier of portfolios.
Predicting Severity in People with Aphasia: A Natural Language Processing and Machine Learning Approach

Speech language pathologists need an accurate assessment of the severity of people with aphasia (PWA) to design and provide the best course of therapy. Currently, severity is evaluated manually by an increasingly scarce pool of experienced and well-trained clinicians, taking considerable time resources. By analyzing the transcripts from three discourse elicitation methods, this study combines natural language processing (NLP) and machine learning (ML) to predict the severity of PWA, both by score and severity level. By engineering language features from PWA tasks, an unstructured k-means clustering presents distinct aphasia types, showing validity of the selected features. We develop regression models to predict severity scores along with a classification of severity by level (Mild, Moderate, Severe, and Very Severe) to assist clinicians to easily plan and monitor the course of treatment. Our best ML regression model uses a deep neural network and results in a mean absolute error (MAE) of 0.0671 and root mean squared error (RMSE) of 0.0922. Our best classification model uses a random forest and result in an overall accuracy of 73%, with the highest accuracy of 87.5% for mild severity. Our results suggest that using NLP and ML provides an accurate and cost-effective approach to evaluate the severity levels in PWA to consequently help clinicians determine rehabilitation procedures.
Assessing the efficiency of irrigation water users' associations and its determinants: evidence from Tunisia

This article analyses the efficiency of water users' associations (WUAs) in the Cap Bon region of Tunisia and studies its main determinants. The analysis is performed in two stages. First, the efficiency is measured via the non‐parametric “data envelopment analysis” (DEA) technique. The DEA models are constructed not only to assess the overall WUA efficiency but also to evaluate the management and engineering sub‐vector efficiencies separately through a mathematical modification in the initial DEA model. In a second stage critical technical and organizational determinants of efficiencies are assessed using a Tobit model. Results show that on average, 18.7% of the used inputs could be saved if the WUAs operated on the frontier. The inefficiencies found can furthermore be mainly attributed to the number of years of experience in operating a WUA, in addition to the number of water pipes managed and the irrigation ratio. The average scale efficiency, which can be calculated as the ratio between constant and variable returns to scale efficiencies, was around 71%, indicating that many WUAs are not operating at an efficient scale. The scale inefficiencies result mainly from administrative and organizational variables. Sub‐vector efficiencies show that on average, the inefficiency of WUAs is more linked with inefficiency in expenditures related to their internal management and functioning, than to engineering inefficiencies. Copyright © 2008 John Wiley & Sons, Ltd.