Miracle-dz/UniFAR-FTUs huggingface dataset:
The Therapeutic Effects of MUC1-C shRNA@Fe3O4 Magnetic Nanoparticles in Alternating Magnetic Fields on Triple-Negative Breast Cancer

Purpose Improving the treatment of triple-negative breast cancer (TNBC) is a serious challenge today. The primary objective of this study was to construct MUC1-C shRNA@ Fe3O4 magnetic nanoparticles (MNPs) and investigate their potential therapeutic benefits in alternating magnetic fields (AMF) on TNBC. Methods Firstly, we verified the high expression of MUC1 in TNBC and synthesized specific MUC1-C shRNA plasmids (MUC1-C shRNA). Then, we prepared and characterized MUC1-C shRNA@Fe3O4 MNPs and confirmed their MUC1-C gene silencing effect and magneto-thermal conversion ability in AMF. Moreover, the inhibitory effects on TNBC in vitro and in vivo were observed as well as biosafety. Finally, the protein levels of BCL-2-associated X protein (Bax), cleaved-caspase3, glutathione peroxidase inhibitor 4 (GPX4), nuclear factor erythroid 2-related factor 2 (NRF2), and ferritin heavy chain 1 (FTH1) in TNBC cells and tissues were examined, and it was speculated that apoptosis and ferroptosis were involved in the synergistic treatment. Results MUC1-C shRNA@ Fe3O4 MNPs have a size of ~75 nm, with an encapsulation rate of (29.78±0.63) %, showing excellent gene therapy and magnetic hyperthermia functions. Under a constant AMF (3Kw) and a set concentration (200µg mL−1), the nanoparticles could be rapidly warmed up within 20 minutes and stabilized at about 43 °C. It could be uptaken by TNBC cells through endocytosis and significantly inhibit their proliferation and migration, with a growth inhibition rate of 79.22% for TNBC tumors. After treatment, GPX4, NRF2, and FTH1 expression levels in TNBC cells and tumor tissues were suppressed, while Bax and cleaved-caspase3 were increased. As key therapeutic measures, gene therapy, and magnetic hyperthermia have shown a synergistic effect in this treatment strategy, with a combined index (q index) of 1.23. Conclusion In conclusion, we developed MUC1-C shRNA@Fe3O4 MNPs with magnetic hyperthermia and gene therapy functions, which have shown satisfactory therapeutic effects on TNBC without significant side effects. This study provides a potential option for the precision treatment of TNBC.
Risk Factors for Hip Fracture After Simple Fall in the Elderly Population

characteristics of individuals who should have a controlled living space where falling can be prevented. Methods: Ambulatory patients aged over 65 were retrospectively analyzed and divided into two groups those with hip fractures (study group) and those without hip fractures after fall (control group). Data were categorized as demographic characteristics, comorbidities, location of trauma, and movement-posture at the time of trauma, chronic drug use. Results: 508 patients were included in the study. There were 179patients in group 1 and 329patients in group 2. There was a significant difference in favor of group 1 in terms of concomitant diabetes, chronic renal disease, cardiac disease, coxarthrosis and smoking. In group 1, the most common place of falling was 'bathroom', while in group 2 it was 'living rooms'. The upper extremity injury of the patients in group 2 was found to be higher than group 1. Conclusion: This study revealed the associated risk factors in hip fractures. Besides, the place where the fall occurred, the activities during the fall, and the accompanying injuries are effective on the occurrence of hip fractures.
Design and synthesis of potential pyrrole-coupled carboxamide derivatives as an anti-superbug MRSA agent

A series of new pyrrole-coupled carboxamide derivatives had been synthesized and amply characterized by various techniques, viz FTIR, 1H-NMR, 13C-NMR, and LCMS. All these compounds had undergone evaluation for their PASS, BBB, pharmacophore model, ADME, and bioactive score. The antibacterial properties of all the derivatives were examined and compound 5i demonstrated strong antibacterial activity against MRSA. A membrane damage investigation supported by SEM pictures, cellular content leakage, potassium efflux, and bacterial respiration suppression showed that compound 5i had anti-MRSA properties. A good binding score of -9.11 was found for compound 5i against the MRSA protein 6FTB in an in silico molecular docking analysis, compared to a score of -10.25 for streptomycin. Based on these findings, compound 5i can be further studied in order to develop it as a drug to treat MRSA infections.
Current Status of Omics Studies Elucidating the Features of Reproductive Biology in Blood-Feeding Insects

Simple Summary Insect vectors are responsible for transmitting a range of diseases, leading to significant mortality rates annually. Their behavior and physiology can undergo shifts due to complex molecular interactions during mating and feeding. In this review, we provide an exhaustive overview of the current “omics” knowledge—spanning genomics, transcriptomics, proteomics, and metabolomics—across various vector species. We highlight potential molecular targets for vector control and outline the advancements and gaps in our understanding, which could pave the way for innovative and effective strategies to curb disease transmission. Abstract Female insects belonging to the genera Anopheles, Aedes, Glossina, and Rhodnius account for the majority of global vector-borne disease mortality. In response to mating, these female insects undergo several molecular, physiological, and behavioral changes. Studying the dynamic post-mating molecular responses in these insects that transmit human diseases can lead to the identification of potential targets for the development of novel vector control methods. With the continued advancements in bioinformatics tools, we now have the capability to delve into various physiological processes in these insects. Here, we discuss the availability of multiple datasets describing the reproductive physiology of the common blood-feeding insects at the molecular level. Additionally, we compare the male-derived triggers transferred during mating to females, examining both shared and species-specific factors. These triggers initiate post-mating genetic responses in female vectors, affecting not only their reproductive success but also disease transmission.
VIEW: Visual-Inertial External Wrench Estimator for Legged Robot

Information about the external wrench in legged robots is crucial for performing interactive tasks. However, most previous research has relied on attaching force/torque sensors to the end-effectors, which has disadvantages in terms of cost and maintenance. Therefore, this article presents a sensorless method for estimating the external wrench on a legged robot. It involves two main steps. First, a nonlinear disturbance observer is used to obtain a noise-reduced ground reaction force estimate. Then, to obtain the applied external wrench value, a factor graph optimization method is implemented to couple the IMU, camera, and estimated leg force data tightly. Unlike the conventional method, the high performance of the optimization approach and the fusion with the exteroceptive sensor improved the estimation accuracy. The effectiveness of the proposed method was verified through several experiments.
Estimating urban residential water demand: Effects of price structure, conservation, and education

Water demand equations are estimated using the most current American Water Works Association (1984) survey of 430 (of 600 largest) U.S. utilities. The data set was augmented by monthly rainfall and temperature data from the National Oceanic and Atmospheric Administration's climatological data. Demographic data were obtained from the U.S. Department of Commerce (1988). Besides the usual endogeneity problems involving block price structures this paper also examines the possible endogeneity of conservation and education programs. Three types of models were used: a marginal price model, an average price model, and Shin's (1985) price perception model. The results generally show that price elasticity is higher in the South and the West. Conservation does not appear to reduce water use, but public education appears to have reduced water usage in the West. The Shin (1985) tests in this study indicate that consumers react more to average than marginal prices in all regions.
Research Methods for Leisure and Tourism: A Practical Guide

1. Introduction: the what, why and who of leisure and tourism research2. Approaches to leisure and tourism research3. Starting out: research plans and proposals4. The range of research methods5. Reviewing the literature6. Secondary data: sources and analysis7. Observation8. Qualitative Methods9. Questionnaire surveys10. Questionnaire design11. Research oractice12. Sampling13. Survey analysis14. Statistical analysis15. Preparing and research reportReferencesIndex
Impact of growing urbanization and air pollution on the regional climate over India

India has witnessed an explosive growth of population (0.3 billion in the year 1950 to 1.04 billion in the year 2002) accompanied by uncontrolled urbanization over the last five decades (www. censusindia.net/, Fig. 1). The population growth has been mainly centered around cities due primarily to the large scale migration of rural population, accelerated by high population growth rates especially in Indo-Gangetic (IG) basin. The result is the IG basin is one of the largest and most densely populated regions of the world (Fig. 2).
Albian Pelagic Rhythms (Piobbico Core)

ABSTRACT The Piobbico core of Aptian-Albian pelagic rhythmites in Italy has been used to explore ways of extracting quantitative time-series of geological, chemical, physical and biological parameters from stratigraphic sequences. Recognition of the precession, obliquity, and eccentricity cycles (the Option frequencies) has permitted time-resolution to the 104-year level. Individually, these time-series curves furnish a potential basis for precise correlations. Collectively, they track the evolution of the depositional system in detail, including its response to orbital forcing. Orbital cycles primarily influenced oceanic fertility and carbonate productivity by foraminifera and coccoliths, as well as the aeration of bottom waters (redox cycle). Three short magnetic reversals occur w thin the Ticinella praeticinensis zone. Frequency spectra of variations in magnetic intensity, inclination and declination yield Milankovitch frequencies which are only partly explained as responses to mineralogy. A computer simulation used Berger's astronomically calculate precession index to drive 1) carbonate flux and 2) depth of bioturbation. This achieved a stratigraphy remarkably similar to that observed. Each step in the computer simulation transfers spectral power from precession to eccentricity. Work on the Piobbico core continues, while Project ALBICORE aims to explore the patterns of rhythmicity in the praeticinensis zone of other areas. End_Page 1164-----------------------
Application of temporal streamflow descriptors in hydrologic model parameter estimation

This paper presents a parameter estimation approach based on hydrograph descriptors that capture dominant streamflow characteristics at three timescales (monthly, yearly, and record extent). The scheme, entitled hydrograph descriptors multitemporal sensitivity analyses (HYDMUS), yields an ensemble of model simulations generated from a reduced parameter space, based on a set of streamflow descriptors that emphasize the timescale dynamics of streamflow record. In this procedure the posterior distributions of model parameters derived at coarser timescales are used to sample model parameters for the next finer timescale. The procedure was used to estimate the parameters of the Sacramento soil moisture accounting model (SAC‐SMA) for the Leaf River, Mississippi. The results indicated that in addition to a significant reduction in the range of parameter uncertainty, HYDMUS improved parameter identifiability for all 13 of the model parameters. The performance of the procedure was compared to four previous calibration studies on the same watershed. Although our application of HYDMUS did not explicitly consider the error at each simulation time step during the calibration process, the model performance was, in some important respects, found to be better than in previous deterministic studies.
Surface Properties of Kuiper Belt Objects and Centaurs

The outer solar system is inhabited by a population of small solar system bodies called Kuiper belt Objects (KBOs) and Centaurs. I present a survey of visible and near infrared (NIR) spectra of the brightest KBOs and Centaurs. The visible spectra of 19 KBOs were obtained at Palomar Observatory. At the W.M. Keck Observatory, NIR spectra were obtained for 33 KBOs and 12 Centaurs. This data marks a significant leap in the available spectra, allowing for the population to be studied as whole. The spectra reveal that most KBOs are covered with a material with characteristics similar to irradiate ices rich in complex organic compounds. Water ice is also observed on some KBOs and Centaurs, though its abundance is variable across the samples. A two end member mixing model is found to describe the NIR spectral properties well, but poorly fits the visible-NIR data. This suggests that the non-water ice component is heterogeneous across the KBO and Centuar populations. To characterize the non-ice component better, the visible spectra are analyzed for signatures of both organic and mineralogical features. No evidence is found for the presence of minerals, but the spectra suggest complex organics are a dominant spectral component. The abundance of water is shown to directly correlate with diameter for KBOs, but not Centaurs, suggesting it is controlled by geophysical processes on KBOs. The analysis of these spectra has revealed an unusual population of KBOs that are now identified as mantle fragments from the large KBO 2003 EL61. The collision that spun-up 2003 EL61, formed its satellite system, and ejected the mantle fragments into the Kuiper belt is discussed. The unusual spectral properties of 2003 EL61, its brightest satellite, and the fragments are examined. Analysis suggests that the surfaces of all these objects can be composed of nearly pure water ice, suggesting that their volatile organic inventory has been lost. The discovery of crystalline water ice on these small KBOs suggests that crystallization is not an indication of recent surface activity as was previously suggested.
A TAILORED DERIVATIVE INSTRUMENT TO MITIGATE THE PRICE-AND-QUANTITY RISK FACED BY WIND POWER COMPANIES

Barbosa, Maria; Street, Alexandre (Advisor); Fânzeres, Bruno (CoAdvisor). A Tailored Derivative Instrument to Mitigate the Price-and-Quantity Risk faced by Wind Power Companies. Rio de Janeiro, 2023. 83p. Dissertação de Mestrado – Departamento de Engenharia Elétrica, Pontifícia Universidade Católica do Rio de Janeiro. The intermittent nature of wind generation combined with the wellknown volatility of electricity spot prices expose Wind Power Companies (WPCs) committed to long-term forward contracts to the so-called price-andquantity risk. Several instruments were designed in the past years to mitigate this risk exposure. However, most of them were mainly constructed to cope with only one of its parts, i.e., price or generation uncertainty. To tackle this issue, in this work, we propose a tailored derivative instrument for WPCs leveraging the principles of options and renewable indexes. The effectiveness and attractiveness of the proposed instrument, referred to as the Wind-Indexed Option (WInd-Op), are evaluated with real data from the Brazilian sector through a general equilibrium setup. We show that Solar Power Companies (SPCs) can be relevant candidates to back these derivatives. Additionally, when compared to the traditional put-and-call options as a benchmark, the results indicate that the equilibrium obtained with the new derivative exhibits a significantly higher total traded volume, lower premium prices, and greater overall welfare.
Morphological Study of Ammonia Parkinsoniana from Laguna Madre and Baffin Bay, Texas

ABSTRACT Three samples of the foraminiferan Ammonia parkinsoniana, from Baffin Bay and Laguna Madre, Texas, were analyzed to determine the effects of temperature and salinity on morphology. Salinity is the more important factor of the two. Specimens collected at a time of higher salinity (50 ppt) differ significantly (p A. parkinsoniana as a distinct species and not an ecophenotypic variant of A. beccarii, because it is, by far, the dominant foraminiferal taxon regardless of salinity or temperature. All specimens appear to be megalospheric.
Drought projection based on a hybrid drought index using Artificial Neural Networks

The Tarim River Basin is a special endorheic arid drainage basin in Central Asia, characterized by limited rainfall and high evaporation as common in deserts, while water is supplied mainly by glacier and snow melt from the surrounding mountains. The existing drought indices can hardly capture the drought features in this region as droughts are caused by two dominant factors (meteorological and hydrological conditions). To overcome the problem, a new hybrid drought index (HDI), integrating the meteorological and hydrological drought regimes, was developed and tested in the basin in the work. The index succeeded in revealing the drought characteristics and the ensemble influence better than the single standardized precipitation index or the hydrological index. The Artificial Neural Network approach based on temperature and precipitation observations was set up to simulate the HDI change. The method enabled constructing scenarios of future droughts in the region using climate simulation of the GCMs under four RCP scenarios from the latest CMIP5 project. The simulations in the study have shown that the water budget patterns in the Tarim River Basin are more sensitive to temperature than to precipitation. Dominated by temperature rise causing an accelerating snow/glacier melt, the frequency of drought months is projected to decrease by about 14% in the next decades (until 2035). The drought duration is expected to be shortened to 3 months on average, with the severity alleviated. However, the region would still suffer more severe droughts with a high intensity in some years. The general decrease in drought frequency and intensity over the region in the future would be beneficial for water resources management and agriculture development in the oases. Copyright © 2014 John Wiley & Sons, Ltd.
Seismic Stratigraphic Mapping of Carbonate Mounds Using 3D Texture Attributes

G-41 SEISMIC STRATIGRAPHIC MAPPING OF CARBONATE MOUNDS USING 3D TEXTURE ATTRIBUTES Summary 1 A procedure for seismic stratigraphic analysis is presented. The procedure lends itself to a set of 3D attributes to capture the seismic stratigraphic patterns inherent in the data. These attributes will be referred to as 3D texture attributes since they are able to describe the reflector-geometry in a small neighborhood. By combining the texture attributes the stratigraphic patterns can be composed. The desired patterns can be input to a 3D- classification system as “training data” and mapped. This procedure will be applied to a case study where
MORPHOLOGICAL CHARACTERISTICS AND CYTOTOXIC ACTIVITY OF THE BLACK SEA STRAIN STREPTOMYCES SP. LIM 10

Aim. The aim of the work was to study the morphological characteristics and cytotoxic activity of the strain of actinobacteria Streptomyces sp. Lim 10 isolated from natural fouling of shell rock in the Odesa Bay of the Black Sea. Materials and methods. The object of the study was a strain of marine actinobacteria Streptomyces sp. Lim 10. The morphology and growth patterns were studied on the media of Gauze 2, Czapek, Eshbi, TSB and on the media of ISP (ISP-1–ISP-7). SG medium was used to accumulate secondary metabolites of actinobacteria. Extraction of exometabolites from the culture liquid was carried out with ethyl acetate followed by dissolution in dimethyl sulfoxide (DMSO). The cytotoxic activity of exometabolites of actinobacteria strain Streptomyces sp. Lim 10 was studied on the model of cultures cells human - rhabdomyosarcoma (RD,) and of the laryngeal adenocarcinoma (Hep-2). The cytotoxic effect of exometabolites of the actinobacteria strain Streptomyces sp. Lim 10 was determined after 24 hours of incubation by microscopy. The degree of destruction of the monolayer was assessed by the number of viable cells in terms of optical density, which was measured using a spectrophotometer at a wavelength of 630 nm. The exometabolites of actinobacteria strain Streptomyces sp. Lim 10 were identified using LC-ESIMS. Results. Study of the morphological properties of the Black Sea strain of actinobacteria Lim 10 showed that it is characterized by pleomorphism. А high cytotoxic activity of exometabolites of the marine strain of actinobacteria Streptomyces sp. Lim 10 was established. The level of cytotoxic effect of metabolites of the strain of actinobacteria Streptomyces sp. Lim 10 on Hep-2 cell culture depended on their concentration. RD cell culture was more sensitive to the cytotoxic effect of exometabolites of Streptomyces sp. Lim 10 – at all tested concentrations of exometabolites (from 2.5 to 500.0 μg/ml), the number of viable cells in the RD monolayer decreased to 51.3 ± 3.0 – 63.7 ± 5.4%, compared to the control. This can be at least partially explained by action of apoptosis-inducing agent staurosporine, which is produced by strain Streptomyces sp. Lim 10 along with itsstructural homologs. Conclusion. Strain Streptomyces sp. Lim 10 is a promising producer of antitumor compounds and can be recommended for further more in-depth studies.
Spontaneous Rupture of Hepatocellular Carcinoma With Fatal Outcome in a Patient Taking Direct-Acting Antivirals

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors globally. Many complications are attributed to it, including spontaneous rupture, which is a serious and rare complication that can be life-threatening. Managing and detecting this condition might pose challenges, especially when there is no prior history of liver cirrhosis or tumor. We report on a 57-year-old man followed as an outpatient for chronic hepatitis C who presented to the emergency department for abdominal pain with abdominal distention and jaundice, occurring two months after treatment by direct-acting antiviral (DAA). He was not known to have a liver tumor on the ultrasound performed before the start of treatment. Therefore, the diagnosis of tumor rupture was not very clear. The evolution was fatal, and death occurred quickly. Although the association between DAA treatment and hepatocarcinogenesis and its possible complications is unknown, close monitoring by high-performance imaging is probably required in patients under DAA.