Miracle-dz/UniFAR-FTUs huggingface dataset:
Does Continuous ELF-EMF Exposure Harms Liver Tissue? A Microscopic Assay

Background: Extremely low frequency electromagnetic ﬁelds (ELF-EMF) are commonly present in daily life all over the world. This study was designed to investigate the eﬀects of the diﬀerent exposure intensity of the low frequency electromagnetic ﬁelds on the liver tissue. Methods: Adult male Wistar rats (n = 30) were randomly allocated in one of the experimental groups including; group A, exposure to 0.5 millitesla (mT) for 2-week (n = 5); group B, exposure to 0.5 mT for 4-week (n = 5); group C, control without radiation (n = 5); groupD,exposureto1mTfor2-week(n=5);groupE,exposureto1mTfor4-week(n=5);andgroupF,shamgroup(n=5). Attheend of the experiment, their liver tissue was dissected and clinically assessed by a pathologist. Results: Our results show that for 1 month of exposure at 1 mT, focal hepatocitolysis and mild to moderate portal inﬂammation is visible and for 2 weeks exposure at this ﬂux density, apoptosis and hepatocyte inﬂammation has occurred. However, for the groups exposed to 0.5 mT, 1 month exposure has caused hepatocyte focal inﬂammation, parenchymal hepatocitolysis, and focal apoptosis while, the exposure for 2 weeks did not aﬀect the liver tissue. Conclusion: Exposure to ELF-EMF at the occupational limit cannot lead to irreversible changes and its eﬀects on cells are enough below moderate changes. At the ﬂux density of 1 mT, eﬀects are moderate and irreversible and they are spread in larger areas than the lower ﬂux density of 0.5 mT. Exposure time correlates with the level of stress on the cells; therefore, for a halftime exposure at occupational level, there exist no changes relative to the control group while for the fulltime exposure, some adverse eﬀects were visible.
RUNNING HEAD: Genomic alignments of mouse and human arthritogenic synovial fibroblasts.

: Genomic responses of mouse synovial fibroblasts during TNF-driven arthritogenesis ABSTRACT Objective. Aberrant activation of synovial fibroblasts (SFs) is a key determinant in the pathogenesis of rheumatoid arthritis (RA). We aimed to produce a map of gene expression and epigenetic changes occurring in this cell type during disease progression in the human TNF-transgenic model of arthritis, and identify commonalities with human SFs. Methods. We used deep sequencing to probe the transcriptome, the methylome and the chromatin landscape of cultured mouse arthritogenic SFs at three stages of disease, as well as SFs stimulated with human TNF. We performed bioinformatics analyses at the gene, pathway and network levels, compared mouse and human data, and validated selected genes in both species. Results. We report that SF arthritogenicity is reflected on distinct dynamic patterns of transcriptional deregulation, enriched in pathways of the innate immune response and mesenchymal differentiation. A functionally-representative subset of these changes is associated with methylation, mostly in gene bodies. The arthritogenic state involves highly active promoters, marked by H3K4 trimethylation. There is significant overlap between mouse and human data, at the level of deregulated genes and to an even higher extent at the level of pathways. Conclusion. This work presents the first systematic examination of the pathogenic changes that occur in mouse synovial fibroblasts in progressive TNF-driven arthritogenesis. Significant correlations with respective human RA SF data further validate the human TNF-transgenic mouse as a reliable model of the human disease. The resource of data generated here may serve as a framework for the discovery of novel pathogenic mechanisms and disease biomarkers.
IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues.

The diverse composition of mammalian tissues poses challenges for understanding the 40 cell-cell interactions required for organ homeostasis and how spatial relationships are perturbed 41 during disease. Existing methods such as single-cell genomics, lacking a spatial context, and 42 traditional immunofluorescence, capturing only 2-6 molecular features, cannot resolve these 43 issues. Imaging technologies have been developed to address these problems, but each 44 possesses limitations that constrain widespread use. Here we report a new method that overcomes 45 major impediments to highly multi-plex tissue imaging. Iterative Bleaching Extends multi-pleXity 46 (IBEX) uses an iterative staining and chemical bleaching method to enable high resolution imaging 47 of >65 parameters in the same tissue section without physical degradation. IBEX can be employed 48 with various types of conventional microscopes and permits use of both commercially available and 49 user-generated antibodies in an ‘open’ system to allow easy adjustment of staining panels based 50 on ongoing marker discovery efforts. We show how IBEX can also be used with amplified staining 51 methods for imaging strongly fixed tissues with limited epitope retention and with oligonucleotide- 52 based staining, allowing potential cross-referencing between flow cytometry, Cellular Indexing of 53 Transcriptomes and Epitopes by Sequencing (CITE-Seq), and IBEX analysis of the same tissue. 54 To facilitate data processing, we provide an open source platform for automated registration of 55 iterative images. IBEX thus represents a technology that can be rapidly integrated into most current 56 laboratory workflows to achieve high content imaging to reveal the complex cellular landscape of 57 diverse organs and tissues. image tissue preparations and a method to facilitate direct integration of the imaging data with flow cytometry and sequencing technologies.
The Cyclin-Dependent Kinase Inhibitor KRP2 Controls the Onset of the Endoreduplication Cycle during Arabidopsis Leaf Development through Inhibition of Mitotic CDKA;1 Kinase Complexes

Exit from the mitotic cell cycle and initiation of cell differentiation frequently coincides with the onset of endoreduplication, a modiﬁed cell cycle during which DNA continues to be duplicated in the absence of mitosis. Although the mitotic cell cycle and the endoreduplication cycle share much of the same machinery, the regulatory mechanisms controlling the transition between both cycles remain poorly understood. We show that the A-type cyclin-dependent kinase CDKA;1 and its speciﬁc inhibitor, the Kip-related protein, KRP2 regulate the mitosis-to-endocycle transition during Arabidopsis thaliana leaf development. Constitutive overexpression of KRP2 slightly above its endogenous level only inhibited the mitotic cell cycle– speciﬁc CDKA;1 kinase complexes, whereas the endoreduplication cycle-speciﬁc CDKA;1 complexes were unaffected, resulting in an increase in the DNA ploidy level. An identical effect on the endoreduplication cycle could be observed by overexpressing KRP2 exclusively in mitotically dividing cells. In agreement with a role for KRP2 as activator of the mitosis-to-endocycle transition, KRP2 protein levels were more abundant in endoreduplicating than in mitotically dividing tissues. We illustrate that KRP2 protein abundance is regulated posttranscriptionally through CDK phosphorylation and proteasomal degradation. KRP2 phosphorylation by the mitotic cell cycle–speciﬁc CDKB1;1 kinase suggests a mechanism in which CDKB1;1 controls the level of CDKA;1 activity through regulating KRP2 protein abundance. In accordance with this model, KRP2 protein levels increased in plants with reduced CDKB1;1 activity. Moreover, the proposed model allowed a dynamical simulation of the in vivo observations, validating the sufﬁciency of the regulatory interactions between CDKA;1, KRP2, and CDKB1;1 in ﬁne-tuning the mitosis-to-endocycle transition.
Context-based techniques for improving database management

The business computer applications abound by a wide variety of “incidents”, some of them are minor and some of them are complex requiring high expert skills and efficient collaboration between experts in order to solve them. These practices reflect the building of operational knowledge, step by step, by Database Administrators (DBAs). They are collected and compared by organizations in order to establish secure procedures for encountered incidents. This kind of procedures can be seen as internal procedures as they have been established within an organization and not delivered by DBMS vendors. In this sense, procedures are collections of safety action sequences allowing solving a given incident in any case. These procedures are based on practices, but eliminate most of contextual information and specific features of each incident. This paper presents techniques for contextualizing database management with a focus on “Contextual Graphs” formalism.
The Pre- and Postsynaptic Roles of Dystroglycan at the Drosophila Neuromuscular Junction are Played by Distinct Protein Isoforms

Dystroglycan has been shown to physically interact with Dystrobrevin via Dystrophin to form the core of the Dystrophin Glycoprotein complex. In this report, we show that Dystroglycan is required for localizing both Dystrophin and Dystrobrevin to the postsynaptic region of the synapse which supports the existence of a Dystrophin Glycoprotein complex at the Drosophila neuromuscular junction. Furthermore, we demonstrate that the absence of Dystroglycan, either pre- or postsynaptically, suppresses the increased neurotransmitter release at the Dys DLP2 E6 and the Dyb 11 mutant NMJs. Dystroglycan mutants display ultrastructural synaptic defects, specifically significantly reduced levels of Synaptotagmin and increased CaMKII expression. Together, these data begin to parse out the different roles of Dystroglycan, Dystrobrevin and Dystrophin at the neuromuscular junction.
Real-time Thread Isolation and Trusted Execution on Embedded RISC-V

—The Internet of Things paradigm has led to an increasing demand for low-power single-core embedded devices with hardware-enforced trusted execution environments (TEE). The preeminent solution in this space is Arm TrustZone, which provides four CPU execution states, each with its own memory and instruction permissions. In 2019, physical memory protection (PMP) instructions were ratiﬁed in the RISC-V ISA, which offers enhancements similar to an Arm MPU, but does not enable the creation of TEE frameworks similar to TrustZone. In this paper, we discuss a limitation in the PMP speciﬁcation which precludes the design of such a TEE framework on RISC-V without resorting to a non-standard hardware modiﬁcation. We propose a simple modiﬁcation to the PMP speciﬁcation which would resolve this limitation without the addition of additional registers. We discuss our early ongoing work in implementing a prototype of this hardware extension and integrating it with the Zephyr real-time operating system (RTOS).
The Secure Data Sharing Mechanism for Cloud Computing Based Cloud Storage System

: Secure cloud storage, which is an emerging cloud service, is designed to protect the confidentiality of outsourced data but also to provide flexible data access for cloud users whose data is out of physical control. The emergence of cloud infrastructure has signiﬁcantly reduced the costs of hardware and software resources in computing infrastructure. To ensure security, the data is usually encrypted before it’s outsourced to the cloud. Unlike searching and sharing the plain data, it is challenging to search and share the data after encryption. Nevertheless, it is a critical task for6 the cloud service provider as the users expect the cloud to conduct a quick search and return the result without losing8 data conﬁdentiality. To overcome these problems, Finally, the proposed construction21 is demonstrated practical and efﬁcient in the performance and property comparison. However, the use of CP-ABE may yield an inevitable security breach which is known as the misuse of access credential (i.e. decryption rights), due to the intrinsic “all-or-nothing” decryption feature of CP-ABE. In this paper, we investigate the two main cases of access credential misuse: one is on the semi-trusted authority side, and the other is on the side of cloud user. To mitigate the misuse, We also present the security analysis and further demonstrate the utility of our system via experiments.
A STUDY ON PREVALENCE AND INCREASING RISK FACTORS OF KIDNEY DISEASE AMONG LOCAL POPULATION OF PAKISTAN

: Introduction: Chronic Kidney Disease (CKD) is progressive loss of renal function. 1 CKD is a great burden on Pakistan's healthcare resources. Lack of screening and risk identification often result in delayed treatment and implementation of preventive measures. Aims and objectives: The basic aim of this study is to find the prevalence and increasing risk factors of kidney disease among local population of Pakistan. Material and methods: This cross sectional study was conducted at Multan during Dec 2017 to April 2018. A routine physical examination was performed and the following information collected: (i) smoking status, food frequency and physical activity (IPAQ, international physical activity questionnaire), co-morbidities (history of stroke, cardiovascular disease, known diabetes, known hypertension); (ii) anthropometry (iii) BP was measured thrice with a calibrated automated device. These all things were done for the selected population of the city. Results: The socio-demographic and clinical factors independently associated with presence of CKD were older age, hypertension, and diabetes, elevated SBP, and raised fasting plasma glucose, raised triglycerides, and history of stroke, (p < 0.05 for each). Conclusion: It is concluded that CKD was independently associated with older age, hypertension, diabetes, raised systolic BP, raised plasma fasting glucose, raised triglycerides, and history of stroke. triglycerides,
Light quality and nutrient availability act in concert to regulate axillary bud outgrowth.

Both modulate the strigolactone signalling pathway affecting production and of this Abstract 38 Plants alter their development in response to changes in their environment. This responsiveness has 39 proven to be a successful evolutionary trait. Here we have tested the hypothesis that two key 40 environmental factors, light and nutrition, are integrated within the axillary bud to promote or 41 suppress the growth of the bud into a branch. Using Petunia hybrida as a model for vegetative 42 branching we manipulated both light quality (as crowding and the red: far red ratio) and phosphate 43 availability, such that the axillary bud at node seven varied from deeply dormant to rapidly growing. 44 In conjunction with the phenotypic characterisation we have also monitored the state of the 45 strigolactone (SL) pathway by quantifying SL-related gene transcripts. Mutants in the SL pathway 46 inhibit but do not abolish the branching response to these environmental signals and neither signal 47 is dominant over the other, suggesting the regulation of branching in response to the environment is 48 complex. We have isolated three new putatively SL-related TCP genes from petunia and have 49 identified that these TCP type transcription factors may have roles in the SL signalling pathway both 50 before and after the reception of the SL signal at the bud. We show that the abundance of the 51 receptor transcript is regulated by the light quality such that axillary buds growing in added far red 52 light have greatly increased receptor transcript abundance. This suggests a mechanism whereby the 53 impact of any SL signal reaching an axillary bud is modulated by the responsiveness of these cells to 54 the signal. 55
A Comparative Study On The Factors Influencing Customer Satisfaction On E-Banking In Trivandrum And Addis Ababa

- Today the refined technology has taken an important place in the upcoming development of financial service, particularly banking sector development is affected more than any other financial provider groups. Increased use of mobile services and internet as a new delivery channel for banking transactions and international trading requires influencing the banking sectors to pay more attention towards factors of e-banking to satisfy customers. Thus, this study assesses the factors affecting customer satisfaction of e-banking with a particular reference of Trivandrum and Addis Ababa Commercial bank customers. To achieve the aim of the study, the descriptive survey method was employed and multi-stage sampling technique was utilized. To this end, a sample size of the study was (n = 835). The collected data were analyzed based on Factor analysis tool. The major finding was that there is significant difference on factors affecting of customer satisfaction on e-banking between Trivandrum and Addis Ababa
Total Ionizing Dose Response of High-k Dielectrics on MOS Devices

As advanced Metal Oxide Semiconductor Field Effect Transistors (MOSFETs) technology continues to minimize the gate oxide thickness, the exponential increase in gate leakage current poses a major challenge for silicon dioxide (SiO2) based devices. In order to reduce the gate leakage current while maintaining the same gate capacitance, alternative gate insulator materials with higher dielectric constant (high-k) became the preferred replacement of SiO2 gate dielectrics. Germanium (Ge) MOSFETs have been regarded as promising candidates for future high-speed applications because they possess higher carrier mobility when compared to silicon based devices. At present, advanced microelectronics devices and circuits are used in aerospace engineering, nuclear industry, and radiotherapy equipment. These applications are unavoidably exposed to space-like radiation, which has a relative low radiation dose rate at 10-2-10-6 rad(Si)/s. For these reasons, it is necessary to understand the low-dose-rate radiation response of high-k materials based on Si and Ge MOS devices. The radiation response of high-k materials such as radiation-induced oxide and interface trap density have been typically examined by carrying out off-site capacitance-voltage (CV) measurements. However, the conventional and off-site radiation response measurements may underestimate the degradation of MOS devices. In this study, a semi-automated laboratory-scale real-time and on-site radiation response testing system was developed to evaluate the radiationresponse. The system is capable of estimating the radiation response of MOS devices whilst the devices are continuously irradiated by -rays raysrays. Moreover, the complete CV characteristics of MOS capacitors were measured in a relatively short time. The pulse CV measurement reduces the impact of charge trapping behavior on the measurement results, when compared to conventional techniques. The total ionizing dose radiation effect on HfO2 dielectric thin films prepared by atomic layer deposition (ALD) has been investigated by the proposed measurement system. The large bidirectional ΔVFB of the irradiated HfO2 capacitor was mainly attributed to the radiation-induced oxide trapped charges, which were not readily compensated by bias-induced charges produced over the measurement timescales of less than 5 ms. Radiation response of Ge MOS capacitors with HfO2 and HfxZr1-xOy gate dielectrics was also investigated. It was found that radiation-induced interface traps were the dominant factor for Flat-band Voltage shift (ΔVFB) in HfO2 thin films, whereas the radiation response for Zr-containing dielectrics under positive bias was mainly affected by oxide traps. Under positive biased irradiation, the Zr-doped HfxZr1-xOy exhibited smaller ΔVFB than that of HfO2. This is attributed to the de-passivation of Ge-S bonds in capacitors incorporating HfO2 thin films, resulting in the build-up of interface traps. Under negative biased irradiation, ΔVFB was attributed to the combined effect of the net oxide trapped charges and the passivation of Ge dangling bonds at the Ge/high-k interface.
Intelligibility of Philippine English to a Selected ASEAN Country – Thailand

This paper is a sequence to a bigger research project that attempts to document the intelligibility of Philippine English (PE) to its neighboring countries, specifically those included in the Association of Southeast Asian Nations (ASEAN) partnership. From this perspective, the study attempted to identify and demonstrate how intelligible PE is to Thais. The study examined acrolectal, mesolectal, and basilectal speakers of PE, and they underwent two tests, the pronunciation task, and the stress task. The listeners were given six tests, three cloze tests, one intelligibility questionnaire, one perception of PE, and one English proficiency self-assessment. These tests were to confirm that they really belong to the lectal category. The results revealed that PE is about 50% intelligible for Thais. The factors that have strongly affected this intelligibility rate are listeners’ familiarity with the variety and the speakers’ effort, choice of words, rate, and pronunciation. It has to be noted that English in Thailand is a foreign language, but then the listeners’ perceived language proficiency did not significantly affect the intelligibility of PE. Moreover, their perception of PE did not affect their understanding of the utterances of the speakers.
Delay-Optimal Distributed Edge Computation Offloading With Correlated Computation and Communication Workloads

Distributed edge computation offloading makes use of distributed wireless edge devices to perform offloaded computation in parallel, which can substantially reduce the computation time. In this article, we explore distributed edge computation offloading where the computation workloads of edge devices are correlated with their communication workloads. In particular, we study the fundamental problem of computation workload allocation and communication scheduling for minimizing the total completion time of the computation offloading. To solve this problem, we need to tackle several challenges due to the precedence constraints of computations and communications, the interference constraints of wireless edge devices, and the correlation between computation and communication workloads. We consider preemptive, half-preemptive, and non-preemptive networks for the formulated problem, respectively. For each setting, we first develop a simplified problem of computation allocation, based on which we then devise an efficient and feasible policy that can arbitrarily approach the optimal policy. For half-preemptive and non-preemptive networks, we also characterize the optimal communication orders. Our results provides useful insights for the computation-communication co-design of distributed edge computation offloading. We evaluate the proposed algorithms using simulation results, which corroborate the advantages of the algorithms.
Influence of Short Term Exercise on the Quality of Life of Patients with Chronic Heart Failure: Case Series from Saudi Arabia

Cardiac rehabilitation (CR) is a comprehensive disease management program for heart failure patients. Most of the heart failure (HF) guidelines are classifying the cardiac rehabilitation for heart failure patients as class 1A which mean highly recommended. However, Arab countries and Middle East are lacking specific CR programs and guidelines. In this study, five cases of chronic heart failure patients were exposed to short term regular exercise for maximum 8 weeks. With a mean of 5.6 exercise visits there are 19. 2% improvement in functional capacity, 35% improvement of exercise distance, 32.5% improvement in quality of life, 25% improvement in quality of sleep and decrease the emergency room visits by 37.5%. The significant improvement was in quality of life, exercise capacity and a decrease in ER visit for all patients.