Miracle-dz/UniFAR-FTUs huggingface dataset:
A Genetically Engineered Bivalent Vaccine Coexpressing a Molecular Adjuvant against Classical Swine Fever and Porcine Epidemic Diarrhea

Classical swine fever (CSF) and porcine epidemic diarrhea (PED) are highly contagious viral diseases that pose a significant threat to piglets and cause substantial economic losses in the global swine industry. Therefore, the development of a bivalent vaccine capable of targeting both CSF and PED simultaneously is crucial. In this study, we genetically engineered a recombinant classical swine fever virus (rCSFV) expressing the antigenic domains of the porcine epidemic diarrhea virus (PEDV) based on the modified infectious cDNA clone of the vaccine strain C-strain. The S1N and COE domains of PEDV were inserted into C-strain cDNA clone harboring the mutated 136th residue of Npro and substituted 3′UTR to generate the recombinant chimeric virus vC/SM3′UTRN-S1NCOE. To improve the efficacy of the vaccine, we introduced the tissue plasminogen activator signal (tPAs) and CARD domain of the signaling molecule VISA into vC/SM3′UTRN-S1NCOE to obtain vC/SM3′UTRN-tPAsS1NCOE and vC/SM3′UTRN-CARD/tPAsS1NCOE, respectively. We characterized three vaccine candidates in vitro and investigated their immune responses in rabbits and pigs. The NproD136N mutant exhibited normal autoprotease activity and mitigated the inhibition of IFN-β induction. The introduction of tPAs and the CARD domain led to the secretory expression of the S1NCOE protein and upregulated IFN-β induction in infected cells. Immunization with recombinant CSFVs expressing secretory S1NCOE resulted in a significantly increased in PEDV-specific antibody production, and coexpression of the CARD domain of VISA upregulated the PEDV-specific IFN-γ level in the serum of vaccinated animals. Notably, vaccination with vC/SM3′UTRN-CARD/tPAsS1NCOE conferred protection against virulent CSFV and PEDV challenge in pigs. Collectively, these findings demonstrate that the engineered vC/SM3′UTRN-CARD/tPAsS1NCOE is a promising bivalent vaccine candidate against both CSFV and PEDV infections.
Preparation and Characterization of Capsaicin Encapsulated Polymeric Micelles and Studies of Synergism with Nicotinic Acids as Potential Anticancer Nanomedicines

Background/Objective/Methods: Capsaicin micelles were prepared by the direct dissolution using the amphiphilic copolymer Pluronic P123 and advanced for substantially novel submicro-nanocytotoxicity. Results: Superior cytotoxicity of capsaicin loaded nanomicelles vs. both the raw capsaicin and reference cisplatin in pancreatic PANC1, breast MCF7, colorectal resistant CACO2, skin A375, lung A549 and prostate PC3 cancer cell lines were delineated. Nicotinic acid (NA) derivative 39 (2-Amino IsoNA) had antiinflammatory potential but consistently lacked antiproliferation in MCF7, PANC1 and CACO2. Besides NA derivatives 8 (5-MethylNA) and 44 (6-AminoNA) exhibited lack of antiinflammation but had comparable antitumorigenesis potency to cisplatin in PANC1 cells. Though capsaicin loaded nanomicelles exerted pronounced antiinflammation (with IC50 value of 510 nM vs. Indomethacin's) in lipopolysacchride-induced inflammation of RAW247.6 macrophages; they lacked DPPH scavenging propensities. Free capsaicin proved more efficacious vs. its loaded nanocarriers to chemosensitize cytotoxicity of combinations with NAs 1(6-Hexyloxy Nicotinic Acid), 5(6-OctyloxyNA), 8(5-MethylNA), 12(6-Thien-2yl-NA), 13(5,6-DichloroNA) and 44(6-AminoNA) in CACO2, PANC1 and prostate PC3. Conclusion: Capsaicin loaded nanomicelles proved more efficacious vs. free capsaicin to chemo-sensitize antiproliferation of cotreatments with NA derivatives, 1, 5, 8, 12, 13 and 44 (in skin A375), 1, 5, 8 and 12 (in breast MCF7), and 1, 5, 12 and 44 (in lung A549).
Elastic orthorhombic anisotropic parameter inversion: An analysis of parameterization

ABSTRACT The resolution of a multiparameter full-waveform inversion (FWI) is highly influenced by the parameterization used in the inversion algorithm, as well as the data quality and the sensitivity of the data to the elastic parameters because the scattering patterns of the partial derivative wavefields (PDWs) vary with parameterization. For this reason, it is important to identify an optimal parameterization for elastic orthorhombic FWI by analyzing the radiation patterns of the PDWs for many reasonable model parameterizations. We have promoted a parameterization that allows for the separation of the anisotropic properties in the radiation patterns. The central parameter of this parameterization is the horizontal P-wave velocity, with an isotropic scattering potential, influencing the data at all scales and directions. This parameterization decouples the influence of the scattering potential given by the P-wave velocity perturbation from the polar changes described by two dimensionless parameter pertur.
The pairwise error probability and precoder design for amplify-and-forward half-duplex cooperative system

In this paper, we derive the exact asymptotic pairwise error probability (PEP) of an AF half-duplex cooperative system in which the maximum likelihood detector is used. Based on our analysis and observations, we propose a unitary precoder design to improve the system performance. We first design a precoder to achieve the full diversity gain function. Then for the case of a 4-QAM signal being transmitted, we further optimize the coding gain, and arrived at a closed-form optimum precoder. Simulations indicate that our proposed precoder design greatly improves the performance of the system
Dirichlet averages of the generalized confluent hypergeometric function

This article deals with the representation of Dirichlet averages of the generalized confluent hypergeometric function (also referred to as a Mittag-Leffler-confluent hypergeometric function), which are presented by means of the Pathway fractional integral operator and of the generalized hypergeometric functions of multiple variables. Additionally, certain particular cases are taken into account when the aforementioned Dirichlet averages coincide with the confluent hypergeometric function and generalized Wright hypergeometric functions.
The proteomic effects of ketone bodies: implications for proteostasis and brain proteinopathies

A growing body of evidence supports the beneficial effects of the ketone bodies (KBs), acetoacetate and β-hydroxybutyrate (BHB), on diverse physiological processes and diseases. Hence, KBs have been suggested as therapeutic tools for neurodegenerative diseases. KBs are an alternative fuel during fasting and starvation as they can be converted to Ac-CoA to produce ATP. A ketogenic diet (KD), enriched in fats and low in carbohydrates, induces KB production in the liver and favors their use in the brain. BHB is the most abundant KB in the circulation; in addition to its role as energy fuel, it exerts many actions that impact the set of proteins in the cell and tissue. BHB can covalently bind to proteins in lysine residues as a new post-translational modification (PTM) named β-hydroxybutyrylation (Kbhb). Kbhb has been identified in many proteins where Kbhb sites can be critical for binding to other proteins or cofactors. Kbhb is mostly found in proteins involved in chromatin structure, DNA repair, regulation of spliceosome, transcription, and oxidative phosphorylation. Histones are the most studied family of proteins with this PTM, and H3K9bhb is the best studied histone mark. Their target genes are mainly related to cell metabolism, chromatin remodeling and the control of circadian rhythms. The role of Kbhb on physiological processes is poorly known, but it might link KB metabolism to cell signaling and genome regulation. BHB also impacts the proteome by influencing proteostasis. This KB can modulate the Unfolded Protein Response (UPR) and autophagy, two processes involved in the maintenance of protein homeostasis through the clearance of accumulated unfolded and damaged proteins. BHB can support proteostasis and regulate the UPR to promote metabolism adaptation in the liver and prevent cell damage in the brain. Also, BHB stimulates autophagy aiding to the degradation of accumulated proteins. Protein aggregation is common to proteinopathies like Alzheimer’s (AD) and Parkinson’s (PD) diseases, where the KD and BHB treatment have shown favorable effects. In the present review, the current literature supporting the effects of KBs on proteome conformation and proteostasis is discussed, as well as its possible impact on AD and PD.
Statistical Downscaling to Predict Monthly Rainfall Using Generalized Linear Model with Gamma Distribution

Statistical Downscaling (SDS) models might involve ill-conditioned covariates (large dimension and high correlation/multicollinear). This problem could be solved by a variable selection technique using L1 regularization/LASSO or a dimension reduction approach using principal component analysis (PCA). In this paper, both methods were applied to generalized linear modeling with gamma distribution and compared to predict rainfall models at 11 rain posts in Indramayu. More over, generalized linear model with gamma distribution was used to obtain non-negative rainfall prediction and compared with principal component regression (PCR). Two types of ill-conditioned data with different characteristics (CMIP5 and GPCP version 2.2) were used as covariates in SDS modeling. The results show that three methods (PCR, Gamma-PC, and Gamma-L1) did not demonstrate significant difference in term of Root Mean Square Error (RMSE) after addition of dummy variables (month) in the models. However, a generalized linear modeling with gamma distribution could be considered as the best methods since it provided non-negative rainfall predictions.
Lumbar Disc Nucleus Replacement for Refractory Back Pain: A Case Report

Back pain is one of the most common sources of pain and disability in the world. The value of surgically treating discogenic low back pain (dLBP) is controversial and currently limited to fusion or total disc replacement. The clinical case presented is a 25-year-old man with 5 years of spontaneous onset low back pain refractory to medical care. The patient was enrolled in a Phase 1 clinical trial designed to test the safety profile of a novel nucleus replacement device. He underwent a left-sided anterior retroperitoneal approach, nuclectomy and implantation of the PerQdisc. At the 2-year follow up period his pain scores had improved significantly. Dynamic radiographs demonstrated 23.4 degrees of segmental motion with no suggestion of inflammation on MRI. This case demonstrates successful diagnosis of dLBP and surgical treatment using a novel motion-sparing and form-fitting nucleus replacement device, in the context of a clinical trial. While the diagnostic criteria used in this case report may be a useful guide to identify other patients with dLBP, the surgical treatment offered should be interpreted with caution until appropriate clinical trials are presented.
Uniform global existence and convergence of Euler-Maxwell systems with small parameters

The Euler-Maxwell system with small parameters arises in the modeling of magnetized plasmas and semiconductors. For initial data close to constant equilibrium states, we prove uniform energy estimates with respect to the parameters, which imply the global existence of smooth solutions. Under reasonable assumptions on the convergence of initial conditions, this allows to show the global-in-time convergence of the Euler-Maxwell system as each of the parameters goes to zero.
On the Modification of Mellin Convolution Operator and Its Associated Information Potential

Abstract In this paper, we define a new generalization of Mellin-Gauss-Weierstrass operators that preserve logarithmic functions. We compute logarithmic moments of the new operators and describe the behavior of the modified operators in some weighted spaces. The weighted approximation properties of operators including weighted approximation and weighted quantitative type approximation properties, using weighted logarithmic modulus of continuity, are presented. Using the Mellin-Gauss-Weierstrass kernel as a logarithmic probability density, we study the associated information potential, the expected value and the variance .
Effects of Adding Chitosan on Drug Entrapment Efficiency and Release Duration for Paclitaxel-Loaded Hydroxyapatite—Gelatin Composite Microspheres

Hydroxyapatite—gelatin microspheres with cone-like pores were synthesized via the wet-chemical method using ammonium dihydrogen phosphate ((NH4)H2PO4) and calcium nitrate (Ca(NO3)2·4H2O) as a source of calcium and phosphate ions with the addition of gelatin, which proved to be more osteoconductive than commercial products, such as fibrin glue and Osteoset® Bone Graft Substitute. Following the method of the previous study for loading paclitaxel (PTX), a drug entrapment efficiency of around 58% was achieved, which is much lower than that of the doxorubicin (DOX)-loaded one. Since PTX is hydrophobic while DOX is hydrophilic, the order of chitosan processing and addition of the solvent were tuned in this study, finally leading to an increase in drug entrapment efficiency of 94%. Additionally, the release duration of PTX exceeded six months. The MTT assay indicated that the effect of drug release on the suppression of cancer cells reached more than 40% after one week, thereby showcasing PTX’s capacity to carry out its medicinal functions without being affected by the loading procedures.
Método de Galerkin descontínuo com penalização de fluxos para problemas elípticos

The discontinuous Galerkin methods developed recently for elliptic equations of second order involve the idea of penalizing the jump of the solution at the elements interfaces. This idea allows to impose the smoothness of the numeric solution of the problem in a weak way and at the same time to stabilize the bilinear form guaranteeing its coerciveness and, consequently, the stability of the numeric solution. However, the penalization of the jump of the numerical solution turns the method non conservative, and, consequently, complicates its possible applications to the computational fluid dynamics, for example. In this work innovators techniques of stabilization of the fluxes of the numeric solution is studied. This techniques were introduced for elliptic problems by A. Romkes, J. Oden and S. Prudhomme (2003) and by E. Burman and A. Ern (2005) for advection-dominated problems. Using the recent results of polynomial approximations of functions from piecewise Sobolev space and the fluxes stabilization, the a priori error analysis of the discontinuous Galerkin method is developed. The error estimates that are optimal in h (parameter of mesh discretization) and suboptimal in p(order of polynomial approximation) are presented. Several numeric experiments are realized to confirm the theoretical rates of convergency and to demonstrate a possible applications to practical problems.
Obesity and recurrent miscarriage: The interconnections between adipose tissue and the immune system

Currently, obesity is considered a global public health problem. It is the main risk factor for noncommunicable diseases and reproductive complications, such as recurrent miscarriage (RM). RM affects approximately 1% of couples of reproductive age, and recent studies suggest that its prevalence is increasing. Immunological abnormalities may be responsible for a significant number of cases of unexplained RM. Obesity is recognized as a chronic low‐grade inflammatory condition. The accumulation of fat in obese adipose tissue promotes changes in the local and systemic immune response. Adipokines, exosomes, micro‐RNAs, lipids, and other factors released or secreted by adipose tissue are responsible for the interconnection between obesity and the immune system. Obesity‐induced dysregulation of the innate and acquired immune response is also involved in the immunopathology of pregnancy loss in patients with unexplained RM. Therefore, understanding the communication pathways between maternal adipose tissue and the immune response in women living with obesity and RM is an important objective. Thus, diagnostic tools and new immunomodulatory therapies may be proposed for the management of patients with concurrent obesity and RM.
Transfert radiatif dans les galaxies à Grand Redshift

L'epoque de la reionisation, qui s'etend pendant le premier milliard d'annees de l'Univers, correspond a la periode ou les premieres etoiles et galaxies apparaissent. Dans ce contexte, l'enjeu majeur de cette these est d'etudier la formation de ces premieres structures et leur retroaction radiative sur leur environnement. Pour cela, j'utilise differentes methodes de simulations numeriques permettant de modeliser le transfert radiatif dans les galaxies, dans un cadre cosmologique.En particulier, les plus petites galaxies seraient celles qui apportent la majorite des photons necessaires a ioniser l'Univers. J'explore cette hypothese dans une premiere partie a l'aide de simulations radio-hydrodynamiques avec le code RAMSES-RT, ciblant trois petites galaxies avec une tres haute resolution spatiale et temporelle. Je me suis d'abord interesse aux mecanismes regulant la production et le transfert de photons ionisants dans les galaxies, et j'ai montre que les explosions de supernovae en sont un facteur essentiel. Ensuite, j'ai explore les proprietes observables derivees a partir de ces simulations.Dans un second temps, j'ai developpe une extension au code Monte Carlo de transfert radiatif MCLya prenant en compte la polarisation du rayonnement et l'emission diffuse dans une simulation. J'ai utilise ce code pour post-traiter une simulation d'un blob Lyman-alpha, une source etendue d'emission Lyman-alpha, et etudier ses proprietes de polarisation a l'aide de pseudo-observations. Contrairement a ce qui etait propose precedemment, j'ai pu montrer que la polarisation n'etait pas un indicateur aussi utile qu'espere pour tracer l'origine des photons Lyman-alpha
Über stochastische Asymptoten und Grenzwerte (Abdruck)

(In Botreff von dem stochastischen Grenzwert die Priorit it Herrn Prof. Camelli zukommt). Ich betrachto in meiner Untersuchung das VerhaLtniss des , Gesetzes der grossen Zahlen •g zu diesen Begriffsbildungen und unternehme weiter eine Reihe von Sittzen uber stochastische Asymptoten un d Grenzwerte abzuleiten. So hat man z.B. (bei endlicher Anzahl von Summanden) sowohl fur, algebraische, wie fur geometrische Addition. Sate 1-2:
Fast Context-Awareness Encoder for LiDAR Point Semantic Segmentation

A LiDAR sensor is a valuable tool for environmental perception as it can generate 3D point cloud data with reflectivity and position information by reflecting laser beams. However, it cannot provide the meaning of each point cloud cluster, so many studies focus on identifying semantic information about point clouds. This paper explores point cloud segmentation and presents a lightweight convolutional network called Fast Context-Awareness Encoder (FCAE), which can obtain semantic information about the point cloud cluster at different levels. The surrounding features of points are extracted as local features through the local context awareness network, then combined with global features, which are highly abstracted from the local features, to obtain more accurate semantic segmentation of the discrete points in space. The proposed algorithm has been compared and verified against other semantic KITTI data algorithms and has achieved state-of-the-art performance. Due to its ability to note fine-grained features on the z-axis in space, the algorithm shows higher prediction accuracy for certain types of objects. Moreover, the training and validation time is short, and the algorithm can meet high real-time requirements for 3D perception tasks.
Significance of Postprocedural Contrast Medium Injection after CT-Guided Abscess Drainage

The aim was to evaluate the additive clinical value of an additional post-procedural control-scan after CT-guided percutaneous abscess drainage (PAD) placement with contrast medium (CM) via the newly placed drain. All CT-guided PADs during a 33-month period were retrospectively analyzed. We analyzed two subgroups, containing patients with and without surgery before intervention. Additionally, radiological records were reevaluated, concerning severe inflammatory response syndrome (SIRS) during the intervention. A total of 499 drainages were placed under CT-guidance in 352 patients. A total of 197 drainages were flushed with CM directly after the intervention, and 51 (26%) showed an additional significant finding. An immediate change of therapy was found in 19 cases (9%). The subgroup that underwent surgery (120 CM-drainages; 32 (27%) additional findings; 13 (11%) immediate changes of therapy) showed no statistically significant difference compared to the subgroup without surgery (77 CM-drainages; 19 (25%) additional findings; 5 (6%) immediate changes of therapy). SIRS occurred in 2 of the 197 flushed drainages (1%) after CM application. An additional scan with CM injection via the newly placed drain revealed clinically significant additional information in almost 26% of the drainages reviewed in this study. In 9% of the cases this information led to an immediate change of therapy. Risks for SIRS are low.
Vibration analysis of a rotating variable thickness bladed disk for aircraft gas turbine engine using generalized differential quadrature method

In this paper, free vibration analysis of rotating variable thickness annular bladed disk suitable to be used in aircraft gas turbine engine is investigated. The numerical generalized differential quadrature method is introduced in this paper as a fast and efficient numerical method to be used for vibration analysis of bladed disks of real gas turbine engines. The boundary conditions are supposed to be similar to those of the real bladed disk used in the aircraft engines i.e. clamped for the inner edge and free for the outer edge. Considering the thickness of the disk to vary as a power function and the blades of the bladed disk to be rigid, the numerical solution is performed and the effects of thickness variation, geometric parameters, angular velocity, and number of blades on the natural frequencies and critical speeds are investigated. The obtained numerical results are compared with those reported in the literature indicating a good agreement.