STUDY OF THE SYNERGISTIC ANTIOXIDANT AND ANTIFUNGAL ACTIVITY OF PHENOLS AND FLAVONOIDS ISOLATED FROM SALICORNIAEUROPAEA AND OPUNITIAFICUS-INDICA MEDICINAL PLANTS

The current study, two types of Iraqi medicinal plants were used Salicorniaeuropaea and Opuntiaficus-indica to determine and investigate the biological activity of synergistic effect as antioxidants and antifungals . Preliminary qualitative disclosures were made for all extracts prepared in the study. The study also determined the quantitative analysis of phenols and flavonoids.the result was highest percentage of phenols in the hot aqueous extract of Salicorniaeuropaea and Opuntiaficus-indica plants were (89 , 87 mg/ml) and flavonoids were (90, 93 mg/ml) respectively,the lowest percentage quantity of phenols and flavonoids was in hexane extract for both plants. The antioxidant activity of Phenols and flavonoids isolated from Salicorniaeuropaea and Opuntiaficus-indica plants was evaluated by inhibiting DPPH activity and ferric ion reduction, where the study showed that Phenols and flavonoids had antioxidant activity compared to ascorbic acid and beta-hydroxy acid( BHA). Where the results showed that phenols and flavonoids isolated from Salicorniaeuropae displacement capacity DPPH at a concentration of 80 reached (92.62 mg/ml , 95.33 mg/ml) and the strength of ferric reduction at the concentration (80 mg/ml) was (91.14 mg/ml ,94.28 mg.ml) respectively, and for the plant Opunitiaficus-indica, the antioxidants also reached for phenols and flavonoids isolated from concentration (80 mg/ml) (93.00 mg/ml , 95.29 mg/ml) and ferric reduce (89.75 mg/ml, 92.85 mg/ml) respectively, and their synergistic activity in antioxidants for scavenging free radicals was (96.50 mg/ml , 97.86 mg/ml) and ferric reduce reached (92.28mg/ml , 96.57mg/ml) The biological antifungal activity of phenols flavonoid isolated from both plants was revealed by using different concentrations against two types of pathogenic fungi clinically isolated from human skin, Candida albicans and Candida parapsilosis . The results showed that flavonoids isolated from Salicorniaeuropaea had the highest fungi-inhibiting activity, as the inhibition ratio was in Candia albicans (15 mm) at a concentration (100 mg/ml), (18 mm) Candida parapsilosis while phenols (14 , 18 mM) at a concentration (100 mg/ml)in albicans and paracelluloserespectivily , the phenols isolated from FicusIndica showed the highest inhibition value against fungi Candida albicansand candidiasis (19 , 17 mm) at a concentration (100 mg/ml). , while synergistic activity of mixture phenols and flavonoids isolated both plants results showed that flavonoids gave the highest value for inhibiting the activity of fungi, which was (20 mM) in Candida albicans and (22 mM) in Paracellulose mushrooms at a concentration (100 mg / ml) and phenol In Candida albicans it was (16 mM) and (19 mM) in Candida paralysis it was at a concentration (100 mg/ml). The study concluded the presence of a relatively large amount of phenols and flavonoids in all extracts of the two plants under study The synergistic effect of a mixture of phenols and flavonoids isolated from the two plants had greater antioxidant and antifungal activity than the phenols and flavonoids for each plant separately.