Miracle-dz/UniFAR-FTUs huggingface dataset:
Biotransformation and Tolerance of Trichoderma spp. to Aromatic Amines, 1 a Major Class of Pollutants 2

28 Trichoderma spp. are cosmopolitan soil fungi that are highly resistant to many toxic compounds. Here, 29 we show that T. virens and T. reesei are tolerant to aromatic amines (AA), a major class of pollutants, 30 including the highly toxic pesticide residue 3,4-dichloroaniline (3,4-DCA). In a previous study, we 31 provided proof-of-concept remediation experiments in which another soil fungus, Podospora 32 anserina , detoxifies 3,4-DCA through its arylamine N -acetyltransferase, a xenobiotic-metabolizing 33 enzyme that enables acetylCoA-dependent detoxification of AA. To assess whether the N -acetylation 34 pathway enables tolerance to AA in Trichoderma spp , we cloned and characterized NATs from T. 35 virens and T. reesei . We characterized recombinant enzymes by determining their catalytic efficiencies 36 toward several toxic AA. Through a complementary approach, we also demonstrate that both 37 Trichoderma species efficiently metabolize 3,4-DCA. Finally, we provide evidence that NAT- 38 independent transformation is solely (in T. virens ) or mainly (in T. reesei ) responsible for the observed 39 removal of 3,4-DCA. We conclude that T. virens and, to lesser extent, T. reesei, likely utilize another 40 unidentified metabolic pathway for detoxification of AA aside from acetylation. This is the first 41 molecular and functional characterization of AA biotransformation in Trichoderma spp. Given the 42 potential of Trichoderma for cleanup of contaminated soils, these results reveal new possibilities in 43 fungal remediation of AA-contaminated soils. major class of occupational or environmental pollutants (17), including the highly toxic pesticide 72 residue 3,4 ‑ dichloroaniline (3,4 ‑ DCA) (18). NATs are intracellular XME that catalyze the transfer of 73 an acetyl group from acetylCoA (AcCoA) to the nitrogen or oxygen atom of arylamine-based 74 xenobiotics and their hydroxylated metabolites (19). However, the role of NATs in fungal degradation 75 mechanisms remains largely unknown. Here, we have characterized two arylamine N - 76 acetyltransferases (NATs) in T. virens and one in T. reesei . Although NAT enzymes contribute to 77 tolerance of T. reesei to AA, we show that, contrary to P. anserina , T. reesei and T. virens may utilize 78 another unidentified metabolic pathway for detoxication of AA aside from acetylation. Given the 79 potential of Trichoderma spp. for remediation of contaminated soils (14), our results offer new 80 perspectives in fungal bioremediation.
An Enhanced Fault Prediction Model for Embedded Software based on Code Churn, Complexity Metrics, and Static Analysis Results

—Software systems evolve over time because of functionality extensions, changes in requirements, optimization of code, ﬁxes for security and reliability bugs, etc., and it is commonly known that software quality assurance is thus a continuous issue and is often extremely time-consuming. Therefore, techniques to obtain early estimates of fault-proneness can help in increasing the efﬁciency and effectiveness of software quality assurance. The ability to predict which components in a large software system are most likely to contain the largest numbers of faults in the next release helps to better manage projects, including early estimation of possible release delays, and affordably guide corrective actions to the quality of the software. This paper extends our previous work, where we demonstrated that the combination of code complexity metrics together with static analysis results allows accurate prediction of fault density and to build classiﬁers discriminating faulty from non-faulty components. The extension presented in this paper augments our predictor and classiﬁer with code churn metrics. We applied our methodology to C++ projects from Daimler’s head unit development. In experiments to separate fault-prone from non-fault-prone components, our new approach achieved a classiﬁcation accuracy of 89% , and the regressor predicted the fault density with an accuracy of 85 . 7% . This is an improvement of 7 . 5% with respect to the accuracy of fault density prediction, and an improvement of 10% to the accuracy of fault classiﬁcation compared to our previous approach that did not take code churn metrics into account.
B7-H6 Promotes NK Cell Cytotoxicity Coating Lymphomas with the NKp30 Ligand Mimicking an Induced Self Phenotype by

Induced self expression of the NKp30 ligand B7-H6 facilitates NK cell-mediated elimination of stressed cells. A fusion protein consisting of the ectodomain of B7-H6 and the CD20 single-chain fragment variable 7D8 was generated to mimic an induced self phenotype required for NK cell-mediated target cell elimination. B7-H6:7D8 had bifunctional properties as reﬂected by its ability to simultaneously bind to the CD20 Ag and to the NKp30 receptor. B7-H6:7D8 bound by CD20 + lymphoma cells activated human NK cells and triggered degranulation. Consequently, the immunoligand B7-H6:7D8 induced killing of lymphoma-derived cell lines as well as fresh tumor cells from chronic lymphocytic leukemia or lymphoma patients. B7-H6:7D8 was active at nanomolar concentrations in a strictly Ag-speciﬁc manner and required interaction with both CD20 and NKp30. Remarkably, NK cell cytotoxicity was further augmented by concomitant activation of Fc g receptor IIIa or NK group 2 member D. Thus, B7-H6:7D8 acted synergistically with the CD20 Ab rituximab and the immunoligand ULBP2:7D8, which was similarly designed as B7-H6:7D8 but engaging the NK group 2 member D receptor. In conclusion, to our knowledge, B7-H6:7D8 represents the ﬁrst Ab-based molecule stimulating NKp30-mediated NK cell cytotoxicity for therapeutic purposes and provides proof of concept that Ag-speciﬁc
High-performance Wireless Sensor Node Design for Water Pipeline Monitoring

—Water utilities owners are facing critical challenges in repairing and maintaining pipeline infrastructure. Leakages in water pipeline infrastructure cost millions of dollars every year. The need for a reliable, continuous and efﬁcient system for pipeline monitoring becomes crucial. Wireless Sensor Network (WSN) is a very promising technology to detect leaks in an autonomous way. In this paper, we present a WSN system for water pipeline monitoring. A wireless sensor node based on Zynq System on Chip is developed and simulated. A leak detection algorithm based on Kalman ﬁlter is also implemented and accelerated using the Zynq platform. The experimental results show that the usage of high-performance platforms is suitable only if the power management techniques are employed or for video applications.
Title The effect of temperature and precipitation on salmonellosis cases in South-East An observational study

: 12 Objective: Foodborne illness in Australia, including salmonellosis, is estimated to cost over 13 AUD$1.25 billion annually. Weather has been identified as influential on salmonellosis 14 incidence as cases increase during summer, however time-series modelling of salmonellosis 15 is challenging because outbreaks cause strong autocorrelation. This study assesses whether 16 switching models are an improved method of estimating weather–salmonellosis associations. 17 Design: We analysed weather and salmonellosis in South-East Queensland between 2004- 18 2013 using two common regression models and a switching model, each with 21-day lags for 19 temperature and precipitation. 20 Results: The switching model best fit the data, as judged by its substantial improvement in 21 DIC over the regression models, less autocorrelated residuals and control of seasonality. The 22 switching model estimated a 5 °C increase in mean temperature and 10 mm precipitation 23 were associated with increases in salmonellosis cases of 45.4% (95% CrI = 40.4%, 50.5%) 24 and 24.1% (95% CrI = 17.0%, 31.6%) respectively. 25 Conclusions: Switching models improve on traditional time-series models in quantifying 26 weather–salmonellosis associations. A better understanding of how temperature and 27 precipitation influence salmonellosis may identify where interventions can be made to lower 28 the health and economic costs of salmonellosis.
Teaching Attitude, Is A Psychological Tendency of Teachers Towards Students, Teaching Process and Relative Teaching

Technology holds pedagogical affordances that can help address low performance in mathematics education. This research study investigated which factors significantly contributed to technology usage by Mathematics teachers. The 155 teachers surveyed were found to be regular users of computers, demonstrating a high opinion of the pedagogical worth of technology and perceiving the tool as being useful for enhancing Mathematic education. However, only a minority was really using technology in their teaching practices. Teachers reiterated the need to be adequately trained in the pedagogical integration of Information and Communication Technology (ICT), insisted on the provision of better computer services in schools, including access to the Internet and solicited the support of parents to accompany their children in their studies. A revised theoretical model was drawn based on the Technology Acceptance Model (TAM) and unified theory of acceptance and use of technology (UTAUT) models, and the two ICT related variables that caused significant variances in technology usage were perceived ease of use and facilitating conditions.
Probing The Neutrino Mass Hierarchy With CMB Weak Lensing

We forecast constraints on cosmological parameters with primary cosmic microwave background (CMB) anisotropy information and weak lensing reconstruction with a future post-Planck CMB experiment, the Cosmic Origins Explorer (COrE), using oscillation data on the neutrino mass splittings as prior information. Our Markov chain Monte Carlo (MCMC) simulations in flat models with a non-evolving equation of state of dark energy w give typical 68 per cent upper bounds on the total neutrino mass of 0.136 and 0.098 eV for the inverted and normal hierarchies, respectively, assuming the total summed mass is close to the minimum allowed by the oscillation data for the respective hierarchies (0.10 and 0.06 eV). Including geometric information from future baryon acoustic oscillation measurements with the complete Baryon Oscillation Spectroscopic Survey, Type Ia supernovae distance moduli from Wide-Field Infrared Survey Telescope (WFIRST) and a realistic prior on the Hubble constant, these upper limits shrink to 0.118 and 0.080 eV for the inverted and normal hierarchies, respectively. Addition of these distance priors also yields per cent-level constraints on w. We find tension between our MCMC results and the results of a Fisher matrix analysis, most likely due to a strong geometric degeneracy between the total neutrino mass, the Hubble constant and w in the unlensed CMB power spectra. If the minimal-mass, normal hierarchy were realized in nature, the inverted hierarchy should be disfavoured by the full data combination at typically greater than the 2σ level. For the minimal-mass inverted hierarchy, we compute the Bayes factor between the two hierarchies for various combinations of our forecast data sets, and find that the future cosmological probes considered here should be able to provide ‘strong’ evidence (odds ratio 12:1) for the inverted hierarchy. Finally, we consider potential biases of the other cosmological parameters from assuming the wrong hierarchy and find that all biases on the parameters are below their 1σ marginalized errors.
Consensus on Microembolus Detection by TCD International on Microembolus Detection

—Transcranial Doppler ultrasound is capable of detecting microembolic material, both gaseous and solid, within the intracranial cerebral arteries. To avoid discrediting this promising and exciting new technique, experts in this field met in January 1997 in Frankfurt, Germany, to discuss the limitations and problems of embolus detection and to determine guidelines for its proper use in clinical practice, as well as in scientific investigations. In particular, the authors suggest that studies report the following parameters: (1) ultrasound device, (2) transducer type and size, (3) insonated artery, (4) insonation depth, (5) algorithms for signal intensity measurement, (6) scale settings, (7) detection threshold, (8) axial extension of sample volume, (9) fast Fourier transform (FFT) size (number of points used), (10) FFT length (time), (11) FFT overlap, (12) transmitted ultrasound frequency, (13) high-pass filter settings, and (14) recording time. There was agreement that no current system of automatic embolus detection has the required sensitivity and specificity for clinical use. ( Stroke
The H 1 -Sucrose Cotransporter NtSUT1 Is Essential for Sugar Export from Tobacco Leaves 1

In many species translocation of sucrose from the mesophyll to the phloem is carrier mediated. A sucrose/H 1 -symporter cDNA, NtSUT1, was isolated from tobacco (Nicotianatabacum) and shown to be highly expressed in mature leaves and at low levels in other tissues, including floral organs. To study the in vivo function of NtSUT1, tobacco plants were transformed with a SUT1 antisense construct under control of the cauliflower mosaic virus 35S promoter. Upon maturation, leaves of transformants expressing reduced amounts of SUT1 mRNA curled downward, and strongly affected plants developed chloroses and necroses that led to death. The leaves exhibited impaired ability to export recently fixed 14 CO 2 and were unable to export transient starch during extended periods of darkness. As a consequence, soluble carbohydrates accumulated and photosynthesis was reduced. Autoradiographs of leaves show a heterogenous pattern of CO 2 fixation even after a 24-h chase. The 14 C pattern does not change with time, suggesting that movement of photosynthate between mesophyll cells may also be impaired. The affected lines show a reduction in the development of the root system and delayed or impaired flowering. Taken together, the effects observed in a seed plant (tobacco) demonstrate the impor-tance of SUT1 for sucrose loading into the phloem via an apoplastic route and possibly for intermesophyll transport as well.
The MODY1 Gene for Hepatocyte Nuclear Factor 4 (cid:2) and a Feedback Loop Control COUP-TFII Expression in Pancreatic Beta Cells (cid:1)

Pancreatic islet beta cell differentiation and function are dependent upon a group of transcription factors that maintain the expression of key genes and suppress others. Knockout mice with the heterozygous deletion of the gene for chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) or the complete disruption of the gene for hepatocyte nuclear factor 4 (cid:1) (HNF4 (cid:1) ) in pancreatic beta cells have similar insulin secretion defects, leading us to hypothesize that there is transcriptional cross talk between these two nuclear receptors. Here, we demonstrate speciﬁc HNF4 (cid:1) activation of a reporter plasmid containing the COUP-TFII gene promoter region in transfected pancreatic beta cells. The stable association of the endogenous HNF4 (cid:1) with a region of the COUP-TFII gene promoter that contains a direct repeat 1 (DR-1) binding site was revealed by chromatin immunoprecipitation. Mutation experiments showed that this DR-1 site is essential for HNF4 (cid:1) transactivation of COUP-TFII. The dominant negative suppression of HNF4 (cid:1) function decreased endogenous COUP-TFII expression, and the speciﬁc inactivation of COUP-TFII by small interfering RNA caused HNF4 (cid:1) mRNA levels in 832/13 INS-1 cells to decrease. This positive regulation of HNF4 (cid:1) by COUP-TFII was conﬁrmed by the adenovirus-mediated overexpression of human COUP-TFII (hCOUP-TFII), which increased HNF4 (cid:1) mRNA levels in 832/13 INS-1 cells and in mouse pancreatic islets. Finally, hCOUP-TFII overexpression showed that there is direct COUP-TFII autorepression, as COUP-TFII occupies the proximal DR-1 binding site of its own gene in vivo. Therefore, COUP-TFII may contribute to the control of insulin secretion through the complex HNF4 (cid:1) /maturity-onset diabetes of the young 1 (MODY1) transcription factor network operating in beta cells.
Impact of Demutualization of Chittagong Stock Exchange on Stability of CASPI, CSE30 and CSCX Index

In light of recent development of Chittagong Stock Exchange (CSE) as demutualized exchange, this research was undertaken to evaluate the immediate impact of demutualization on the CASPI, CSE30 and CSCX Index. This study employs line chart to show the relative trend and Pearson Correlation to examine the correlation among the selected indices. Further, this study estimates growth rate, standard deviation and coefficient of variation to evaluate and compare the relative volatility of the selected indices between the period of pre-demutualization and post-demutualization. Null Hypotheses are formulated and tested by One-way ANOVA and Independent Sample T-test, to compare the equivalency of means of selected indices. This study reports during pre-demutualization and post-demutualization period, the selected indices were highly positively correlated. This study also indicates that the differences between mean index value of CASPI, CSE30 and CSCX during pre-demutualization and post-demutualization period are statistically significant. Further, this study finds that mean value of the selected three indices shows positive growth and less volatility after demutualization. Hence, these academic findings are suggestive of renewed confidence among investors indicated through stability of these indices after demutualization.
A Study on Resistivity Behaviour of Conductive Rubber Sheet (CRS) and Quantum Tunnelling Composites (QTC) Pills for Tactile Sensor Application

– This paper presents the work of investigating and comparing the uses of CRS and QTC Pills as a tactile sensor material for Robotic Hand application. The materials were tested for their resistivity characteristics to determine the data reproducibility of the materials. The experiments were conducted based on three parameters; the supply voltage, the separation gap, and the sensor construction against the force/ load that had been exerted onto the materials. The results showed that CRS could cover the lower loading range when compared to QTC Pills. However, the data reproducibility of QTC Pills was better compared to those of CRS, thus making it more suitable to be used as a material for tactile sensing application. Therefore, this paper highlights the potential of these two materials as a tactile sensing transducer, and later, can be used as a useful guideline when designing a tactile sensor with these materials. Copyright © 2015 Penerbit Akademia Baru - All rights reserved.
TACIT KNOWLEDGE IN INTERIOR ARCHITECTURE: INTERIOR ARCHITECTS ON THE DESIGNER – PAYING CLIENT – USER CLIENT RELATIONSHIP

During the last decades, different authors have argued that interior architecture lacked a specific body of knowledge, especially in relationship to architecture (Abercrombie, 1990; Marshall-Baker, 2000; Clemons & Eckman, 2008). The article argues that this gap can be partially bridged by combining theoretical knowledge with insights into the actual design processes. In the actual process of designing interior spaces, some kind of knowledge production is involved (Heylighen et al., 2007), for which designers can rely on different knowledge types, such as ‘explicit’ and ‘tacit’ knowledge (Polanyi, 1967; Collins, 2010; Friedman, 2000). As the discipline of interior architecture is seeking a stronger body of theory (Marshall-Baker, 2000; Clemons & Eckman, 2008), it seems particularly interesting ‘ to bring tacit knowledge into articulate focus ’ (Friedman, 2000, p.13). Therefore, this paper aims to explore the concept of tacit knowledge and its particular value for interior architecture, and then, to present the results of in-depth interviews with interior architects, who have been involved in the process of designing commercial interiors.
D-branes on T 4 /ℤ 2 and T-Duality

: In this note we discuss D-branes on T 4 = Z 2 using the boundary states formalism. Explicit formulas for the untwisted boundary states inherited from the underlying T 4 and twisted states corresponding to branes wrapping collapsed 2-cycles at the orbifold singularities are given. The exact CFT description of the orbifold makes it possible to study how the boundary states transform under R i 7! 1 R i transformation on all directions of the underlying T 4 . We compare their transformation law with results obtained from world volume considerations.
SECOND ORDER CONFIRMATORY FACTOR ANALYSIS OF SMART AGRO-INDUSTRY MANAGEMENT METHODS TO INCREASE PRODUCTIVITIES AND QUALITIES

Nowadays, the agricultural sector is encountering a lot of problems that need to be changed abruptly to become sustainable. The objective of this research is to study the smart agro-industry management methods to increase productivities and qualities. The sample group consisted of 500 agro-industry businesses. Second order confirmatory factor analysis was employed to acquire 5 components, namely Innovation, Knowledge Management, Information Technology, Resources, and Process. The results of the model analysis showed its result passed the assessment criteria in accordance with the empirical data and it was also found that Chi-square Probability Level was 0.061, Relative Chi-square was 1.136, Goodness of fit Index was 0.952 and Root Mean Square Error of Approximation was 0.017.
ISSN 2320-5407 International Journal of Advanced Research (2016), Volume 4, Issue 1, 239- 248

S.K. Goyal Water is the universal component, needed by human beings everyday for various purposes like drinking, washing, bathing, in agricultural lands etc. Burgeoning population increases the demand for industrial establishments to meet human needs, which in turn has created many environment problems, including water pollution. Microbial contamination of fresh water bodies is also a common problem, as water from most of the water bodies requires disinfectant, particularly when water is used for human consumption. Presence of particular type of microbes decides which type of disinfectant will be suitable for eliminating microbial contamination. The water disinfection problem is large and complex in developing countries. Among the various methods of disinfection, chlorine continues to be one of the favorite disinfectants, worldwide. However, it had been shown that using chlorine might produce various deleterious compounds such as Trihalomethanes (THM’s), which were reported to be carcinogenic in nature. The present paper mainly expresses the potential of Emerald zoysia grass in treating water contaminated with coliforms. Further, techno-economical feasibility of its use as a substitute for chlorine in water disinfection is being looked into.