Miracle-dz/UniFAR-FTUs huggingface dataset:
Plant-Derived Antimicrobial Peptides as Potential Antiviral Agents in Systemic Viral Infections

Numerous studies have led to a better understanding of the mechanisms of action of viruses in systemic infections for the development of prevention strategies and very promising antiviral therapies. Viruses still remain one of the main causes of human diseases, mainly because the development of new vaccines is usually challenging and drug resistance has become an increasing concern in recent decades. Therefore, the development of potential antiviral agents remains crucial and is an unmet clinical need. One abundant source of potential therapeutic molecules are plants: they biosynthesize a myriad of compounds, including peptides which can have antimicrobial activity. Our objective is to summarize the literature on peptides with antiviral properties derived from plants and to identify key features of these peptides and their application in systemic viral infections. This literature review highlights studies including clinical trials which demonstrated that plant cyclotides have the ability to inhibit the growth of viruses causing human diseases, defensin-like peptides possess anti-HIV-1 activity, and lipid transfer proteins and some lectins exhibit a varied antimicrobial profile. To conclude, plant peptides remain interesting to explore in the context of emerging and re-emerging infectious diseases.
Investigation on the Model-Based Control Performance in Vehicle Safety Critical Scenarios with Varying Tyre Limits

In recent years the increasing needs of reducing the costs of car development expressed by the automotive market have determined a rapid development of virtual driver prototyping tools that aims at reproducing vehicle behaviors. Nevertheless, these advanced tools are still not designed to exploit the entire vehicle dynamics potential, preferring to assure the minimum requirements in the worst possible operating conditions instead. Furthermore, their calibration is typically performed in a pre-defined strict range of operating conditions, established by specific regulations or OEM routines. For this reason, their performance can considerably decrease in particularly crucial safetycritical situations, where the environmental conditions (rain, snow, ice), the road singularities (oil stains, puddles, holes), and the tyre thermal and ageing phenomena can deeply affect the adherence potential. The objective of the work is to investigate the possibility of the physical model-based control to take into account the variations in terms of the dynamic behavior of the systems and of the boundary conditions. Different scenarios with specific tyre thermal and wear conditions have been tested on diverse road surfaces validating the designed model predictive control algorithm in a hardware-in-the-loop real-time environment and demonstrating the augmented reliability of an advanced virtual driver aware of available information concerning the tyre dynamic limits. The multidisciplinary proposal will provide a paradigm shift in the development of strategies and a solid breakthrough towards enhanced development of the driving automatization systems, unleashing the potential of physical modeling to the next level of vehicle control, able to exploit and to take into account the multi-physical tyre variations.
Influence of Dental Glass Fibers and Orthopedic Mesh on the Failure Loads of Polymethyl Methacrylate Denture Base Resin

The aim of the present study was to evaluate the fracture loads of polymethyl methacrylate (PMMA) complete denture bases reinforced with glass-fiber mesh and orthopedic casting tape (OCT) in comparison to conventional PMMA dentures under artificial aging. Dental fiberglass framework (Group 1) and OCT (Group 2 and 3) reinforced PMMA acrylic dentures were fabricated on the edentulous ridge. Ten PMMA dentures without reinforcement (Group 4) were included as controls. All specimens were placed in a chewing simulator chamber, and fatigue load was applied. To assess the fracture loads, static loads with a universal testing machine were applied. Fractured specimens in each group were evaluated under a scanning electron microscope. The data were statistically analyzed employing analysis of variance and Tukey post-hoc test. The association of denture weight and thickness on fracture load was assessed using Pearson and Spearman correlations. Dental fiberglass (Group 1) displayed the highest fracture load (692.33 ± 751.41 N), and Group 4 (control) exhibited the lowest fracture loads (281.41 ± 302.51 N). Dentures reinforced with fiberglass mesh framework exhibited intact fractures. In contrast, Group 2 and 3 specimens using OCT demonstrated ditching fractures. It was observed that the thickness and weight of all the reinforced specimens influenced the load required to fracture the dentures (p < 0.001). Denture specimens strengthened with OCT (Groups 2 and 3) exhibited failure loads lower than dental fiberglass (Group 1) specimens but higher than unreinforced controls.
The Application of the Essential Oils of Thymus vulgaris L. and Crithmum maritimum L. as Biocidal on Two Tholu Bommalu Indian Leather Puppets

The chemical profile of the Thymus vulgaris (Lamiaceae) essential oil (EO) was investigated in order to evaluate its biological properties against microorganisms affecting two Tholu Bommalu, typical Indian leather puppets stored at the International Puppets Museum “Antonio Pasqualino” of Palermo, Italy. A GC–MS analysis, using both polar and apolar columns, was used to determine the chemical composition of the essential oil. The aim of this study was to evaluate the antimicrobial effectiveness of the Thymus vulgaris and Crithmum maritimum essential oils in vapor phase to disinfect heritage leather puppets. Pieces of leather artifacts that were affected by different bacterial colonies were exposed to EO under vacuum and static evaporation conditions. The results presented showed that the vaporization of essential oil was an efficient method in the disinfection of natural skins, eradicating microorganism in short times. T. vulgaris EO in the 50% solution showed excellent inhibitory activity against isolated bacteria with both methods, but the obtained results suggest that the vacuum method allowed for faster exposition of the artifacts to the biocide. Furthermore, the biocidal properties of the essential oil of a Sicilian accession of Crithmum maritimum (Apiaceae) aerial parts were compared and investigated. The results of the latter essential oil showed a poor activity against the isolated micro-organisms.
Alphavirus Virulence Determinants

Alphaviruses are important pathogens that continue to cause outbreaks of disease in humans and animals worldwide. Diseases caused by alphavirus infections include acute symptoms of fever, rash, and nausea as well as chronic arthritis and severe-to-fatal conditions including myocarditis and encephalitis. Despite their prevalence and the significant public health threat they pose, there are currently no effective antiviral treatments or vaccines against alphaviruses. Various genetic determinants of alphavirus virulence, including genomic RNA elements and specific protein residues and domains, have been described by researchers to play key roles in the development of disease, the immune response to infection, and virus transmissibility. Here, we focus on the determinants that are currently described in the literature. Understanding how these molecular determinants shape viral infections can lead to new strategies for the development of therapies and vaccines to combat these viruses.
Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels

Insulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can provide a form of therapy available to every patient. We prepared different gel formulations using Carbopol® UltrezTM 10, Carbopol® UltrezTM 30, methyl cellulose, and glycerin ointment. The hormone concentration was 1 mg/g of the hydrogel. We assessed the influence of model hydrogels on the pharmaceutical availability of insulin in vitro, and we examined the rheological and the texture parameters of the prepared formulations. Based on spectroscopic methods, we evaluated the influence of model hydrogels on secondary and tertiary structures of insulin. The analysis of rheograms showed that hydrogels are typical of shear-thinning non-Newtonian thixotropic fluids. Insulin release from the formulations occurs in a prolonged manner, providing a longer duration of action of the hormone. The stability of insulin in hydrogels was confirmed. The presence of model hydrogel carriers affects the secondary and the tertiary structures of insulin. The obtained results indicate that hydrogels are promising carriers in the treatment of diabetic foot ulcers. The most effective treatment can be achieved with a methyl cellulose-based insulin preparation.
Optimization of a Totally Fiber-Reinforced Plastic Composite Sandwich Construction of Helicopter Floor for Weight Saving, Fuel Saving and Higher Safety

The application of fiber-reinforced plastic (FRP) composites as structural elements of air vehicles provides weight saving, which results in a reduction in fuel consumption, fuel cost, and air pollution, and a higher speed. The goal of this research was to elaborate a new optimization method for a totally FRP composite construction for helicopter floors. During the optimization, 46 different layer combinations of 4 different FRP layers (woven glass fibers with phenolic resin; woven glass fibers with epoxy resin; woven carbon fibers with epoxy resin; hybrid composite) and FRP honeycomb core structural elements were investigated. The face sheets were composed of a different number of layers with cross-ply, angle-ply, and multidirectional fiber orientations. During the optimization, nine design constraints were considered: deflection; face sheet stress (bending load, end loading); stiffness; buckling; core shear stress; skin wrinkling; intracell buckling; and shear crimping. The single-objective weight optimization was solved by applying the Interior Point Algorithm of the Matlab software, the Generalized Reduced Gradient (GRG) Nonlinear Algorithm of the Excel Solver software, and the Laminator software. The Digimat-HC software solved the numerical models for the optimum sandwich plates of helicopter floors. The main contribution is developing a new method for optimizing a totally FRP composite sandwich structure—due to its material constituents and construction—that is more advantageous than traditional helicopter floors. A case study validated this fact.
Importance of Viral Late Domains in Budding and Release of Enveloped RNA Viruses

Late assembly (L) domains are conserved sequences that are necessary for the late steps of viral replication, acting like cellular adaptors to engage the ESCRT membrane fission machinery that promote virion release. These short sequences, whose mutation or deletion produce the accumulation of immature virions at the plasma membrane, were firstly identified within retroviral Gag precursors, and in a further step, also in structural proteins of many other enveloped RNA viruses including arenaviruses, filoviruses, rhabdoviruses, reoviruses, and paramyxoviruses. Three classes of L domains have been identified thus far (PT/SAP, YPXnL/LXXLF, and PPxY), even if it has recently been suggested that other motifs could act as L domains. Here, we summarize the current state of knowledge of the different types of L domains and their cellular partners in the budding events of RNA viruses, with a particular focus on retroviruses.
Strategies to Optimise Oncolytic Viral Therapies: The Role of Natural Killer Cells

Oncolytic viruses (OVs) are an emerging class of anti-cancer agents that replicate selectively within malignant cells and generate potent immune responses. Their potential efficacy has been shown in clinical trials, with talimogene laherparepvec (T-VEC or IMLYGIC®) now approved both in the United States and Europe. In healthy individuals, NK cells provide effective surveillance against cancer and viral infections. In oncolytic viral therapy, NK cells may render OV ineffective by rapid elimination of the propagating virus but could also improve therapeutic efficacy by preferential killing of OV-infected malignant cells. Existing evidence suggests that the overall effect of NK cells against OV is context dependent. In the past decade, the understanding of cancer and OV biology has improved significantly, which helped refine this class of treatments in early-phase clinical trials. In this review, we summarised different strategies that have been evaluated to modulate NK activities for improving OV therapeutic benefits. Further development of OVs will require a systematic approach to overcome the challenges of the production and delivery of complex gene and cell-based therapies in clinical settings.
Point Cloud Hand–Object Segmentation Using Multimodal Imaging with Thermal and Color Data for Safe Robotic Object Handover

This paper presents an application of neural networks operating on multimodal 3D data (3D point cloud, RGB, thermal) to effectively and precisely segment human hands and objects held in hand to realize a safe human–robot object handover. We discuss the problems encountered in building a multimodal sensor system, while the focus is on the calibration and alignment of a set of cameras including RGB, thermal, and NIR cameras. We propose the use of a copper–plastic chessboard calibration target with an internal active light source (near-infrared and visible light). By brief heating, the calibration target could be simultaneously and legibly captured by all cameras. Based on the multimodal dataset captured by our sensor system, PointNet, PointNet++, and RandLA-Net are utilized to verify the effectiveness of applying multimodal point cloud data for hand–object segmentation. These networks were trained on various data modes (XYZ, XYZ-T, XYZ-RGB, and XYZ-RGB-T). The experimental results show a significant improvement in the segmentation performance of XYZ-RGB-T (mean Intersection over Union: 82.8% by RandLA-Net) compared with the other three modes (77.3% by XYZ-RGB, 35.7% by XYZ-T, 35.7% by XYZ), in which it is worth mentioning that the Intersection over Union for the single class of hand achieves 92.6%.
Comparative Safety Profiles of Sedatives Commonly Used in Clinical Practice: A 10-Year Nationwide Pharmacovigilance Study in Korea

This study aims to compare the prevalence and seriousness of adverse events (AEs) among sedatives used in critically ill patients or patients undergoing invasive procedures and to identify factors associated with serious AEs. Retrospective cross-sectional analysis of sedative-related AEs voluntarily reported to the Korea Adverse Event Reporting System from 2008 to 2017 was performed. All AEs were grouped using preferred terms and System Organ Classes per the World Health Organization—Adverse Reaction Terminology. Logistic regression was performed to identify factors associated with serious events. Among 95,188 AEs, including 3132 (3.3%) serious events, the most common etiologic sedative was fentanyl (58.8%), followed by pethidine (25.9%). Gastrointestinal disorders (54.2%) were the most frequent AEs. The most common serious AE was heart rate/rhythm disorders (33.1%). Serious AEs were significantly associated with male sex; pediatrics; etiologic sedative with etomidate at the highest risk, followed by dexmedetomidine, ketamine, and propofol; polypharmacy; combined sedative use; and concurrent use of corticosteroids, aspirin, neuromuscular blockers, and antihistamines (reporting odds ratio > 1, p < 0.001 for all). Sedative-induced AEs are most frequently reported with fentanyl, primarily manifesting as gastrointestinal disorders. Etomidate is associated with the highest risk of serious AEs, with the most common serious events being heart rate/rhythm disorders.
Applied anatomy of the skull in the Arabian horse: A computed tomographic, cross‐sectional, volumetric and morphometric study

Abstract This study was conducted to present a comprehensive and integrative computed tomography (CT) – anatomical cross sections atlas of skull, volumetric properties of the paranasal sinuses, and morphometric values for surface cranial nerves in the adult Arabian horse. Ten heads of Arabian horse breed were used. The different structures in the nasal, oral and cranial cavities were determined and labelled in the anatomical sections and their corresponding CT scan images. Three paranasal sinuses namely maxillary, conchofrontal and sphenopalatine sinuses were identified in the CT scan images. The caudal maxillary sinus was the largest paranasal sinus with 131.93 ± 7.67 cm3 volume and the sphenopalatine sinus 13.3 ± 1.2 cm3 volume was the smallest one. The infraorbital foramen was located 4.16 ± 0.18 cm and 4.70 ± 0.35 cm far away from the most rostral point of the facial crest and alveolar root, respectively. The mean distance between the mental foramen and most lateral incisive tooth was 3.12 ± 0.29 cm. These results including present CT scan‐cross‐sectional atlas, paranasal sinuses volume and morphometric properties would be applicable in practice for more precise diagnosis of head lesions and blocking the surface terminal branches of the cranial nerves during surgical operations in this valuable horse's breed.
Preparation and Characterization of Salt-Mediated Injectable Thermosensitive Chitosan/Pectin Hydrogels for Cell Embedding and Culturing

In recent years, growing attention has been directed to the development of 3D in vitro tissue models for the study of the physiopathological mechanisms behind organ functioning and diseases. Hydrogels, acting as 3D supporting architectures, allow cells to organize spatially more closely to what they physiologically experience in vivo. In this scenario, natural polymer hybrid hydrogels display marked biocompatibility and versatility, representing valid biomaterials for 3D in vitro studies. Here, thermosensitive injectable hydrogels constituted by chitosan and pectin were designed. We exploited the feature of chitosan to thermally undergo sol–gel transition upon the addition of salts, forming a compound that incorporates pectin into a semi-interpenetrating polymer network (semi-IPN). Three salt solutions were tested, namely, beta-glycerophosphate (βGP), phosphate buffer (PB) and sodium hydrogen carbonate (SHC). The hydrogel formulations (i) were injectable at room temperature, (ii) gelled at 37 °C and (iii) presented a physiological pH, suitable for cell encapsulation. Hydrogels were stable in culture conditions, were able to retain a high water amount and displayed an open and highly interconnected porosity and suitable mechanical properties, with Young’s modulus values in the range of soft biological tissues. The developed chitosan/pectin system can be successfully used as a 3D in vitro platform for studying tissue physiopathology.
Influence of Curly Leaf Trait on Cottonseed Micro-Nutrient Status in Cotton (Gossypium hirsutum L.) Lines

Cottonseed is a source of nutrients, including protein, oil, and macro- and micro-nutrients. Micro-nutrients such as boron (B), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) are essential for plant and human health. Deficiencies of these micro-nutrients in soil lead to poor crop production and poor seed quality. Micro-nutrient deficiencies in the human diet lead to malnutrition and serious health issues. Therefore, identifying new cotton lines containing high nutritional qualities such as micro-nutrients, and understanding plant traits influencing micro-nutrients are essential. The objective of this research was to investigate the effects of leaf shape (curly leaf: CRL) on cottonseed B, Cu, Fe, Mn, Ni (nickel), and Zn in two near-isogenic cotton lines differing in leaf shape (DP 5690 wild-type with normal leaves and DP 5690 CRL). We also used Uzbek CRL, the source of the curly leaf trait, for comparison. A field experiment was conducted in 2014 and 2015 in Stoneville, MS, USA. The experiment was a randomized complete block design with three replicates. The results showed that, in 2014, both DP 5690 wild-type and Uzbek CRL had higher seed B, Cu, Fe, and Ni than in DP 5690 CRL. The accumulation of Mn and Zn in seeds of DP 5690 CRL was higher than in DP 5690 wild-type and Uzbek CRL. However, in 2015, the concentrations of B, Cu, Fe, and Ni, including Mn and Zn, were higher in both DP 5690 wild-type and Uzbek CRL than in DP 5690 CRL. Positive and negative correlations existed in 2014; however, only positive correlations existed between all nutrients in 2015. This research demonstrated that leaf shape can alter cottonseed micro-nutrients status. As Uzbek CRL behaved similar to wild-type, both leaf shape and other factors contributed to the alteration in seed micronutrients, affecting seed nutritional qualities. Therefore, leaf-shape partially contributed to the changes in micro-nutrients in cottonseed. The negative and positive correlations in 2014, and only positive correlations in 2015, were likely due to the heat difference between 2014 and 2015 as 2015 was warmer than 2014. Significant levels of seed micro-nutrients were shown between these lines, providing opportunities for breeders to select for high seed micro-nutrients in cotton. Additionally, the current research provides researchers with physiological information on the impact of leaf shape on seed nutritional quality. The leaf shape trait can also be used as a tool to study leaf development, physiological, biochemical, and morphological processes.
Prognostic value of stress echocardiography assessed by the ABCDE protocol

Abstract Aim The aim of this study was to assess the prognostic value of ABCDE-SE in a prospective, large scale, multicentre, international, effectiveness study. Stress echocardiography (SE) was recently upgraded to the ABCDE protocol: step A, regional wall motion abnormalities; step B, B lines; step C, left ventricular contractile reserve; step D, Doppler-based coronary flow velocity reserve in left anterior descending coronary artery; and step E, electrocardiogram-based heart rate reserve. Methods and results From July 2016 to November 2020, we enrolled 3574 all-comers (age 65 ± 11 years, 2070 males, 58%; ejection fraction 60 ± 10%) with known or suspected chronic coronary syndromes referred from 13 certified laboratories. All patients underwent clinically indicated ABCDE-SE. The employed stress modality was exercise (n = 952, with semi-supine bike, n = 887, or treadmill, n = 65 with adenosine for step D) or pharmacological stress (n = 2622, with vasodilator, n = 2151; or dobutamine, n = 471). SE response ranged from score 0 (all steps normal) to score 5 (all steps abnormal). All-cause death was the only endpoint. Rate of abnormal results was 16% for A, 30% for B, 36% for C, 28% for D, and 37% for E steps. During a median follow-up of 21 months (interquartile range: 13–36), 73 deaths occurred. Global X2 was 49.5 considering clinical variables, 50.7 after step A only (P = NS (not significant)) and 80.6 after B–E steps (P < 0.001 vs. step A). Annual mortality rate ranged from 0.4% person-year for score 0 up to 2.7% person-year for score 5. Conclusion ABCDE-SE allows an effective prediction of survival in patients with chronic coronary syndromes.