Miracle-dz/UniFAR-FTUs huggingface dataset:
Persistent Behavioral Deficits in Rats after Parasagittal Fluid Percussion Injury

Abstract Although traumatic brain injury (TBI) is now considered a chronic disease, few studies have investigated the long-term behavioral deficits elicited by a well-established rodent model of injury. Here we evaluate behavioral measures, commonly used in TBI research, to determine which tests are useful for studying long-term effects of brain injury in rats. Male Sprague-Dawley rats were handled and pre-trained to neurological, balance, and motor coordination tests prior to receiving parasagittal fluid-percussion injury (FPI), sham injury, or maintenance as naive cohorts. Rats underwent neuroscore, beam-balance, and beam-walk tests for 3 days after injury. Subsequently, in separate groups at 3, 6, or 12 months, they were re-tested on the same tasks followed by a working memory version of the Morris water maze. On post-injury days (PIDs) 1–3, significant effects of injury on neuroscore, beam-balance, and beam-walk were observed. Differences in the beam-walk task were not detectable at any of the later t.
in a CRISPR System.

SUMMARY CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreignderived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. Most type V-C and V-D systems lack the cas2 gene and have unusually short CRISPR repeats and spacers. Here, we show that a miniintegrase comprising the type V-C Cas1 protein alone catalyzes DNA integration with a preference for short (17–19 base pair) DNA fragments. The mini-integrase has weak specificity for the CRISPR array. We present evidence that the Cas1 proteins form a tetramer for integration. Our findings support a model of a minimal integrase with an internal ruler mechanism that favors shorter repeats and spacers. This minimal integrase may represent the function of the ancestral Cas1, prior to Cas2 adoption. Abstract Wright et al. demonstrate the function of an integrase comprised of a single protein, Cas1, that inserts unusually short fragments of viral DNA into the bacterial genome as part of the CRISPRCas adaptive immunity pathway. This minimal integrase may represent the function of the ancestral Cas1, prior to Cas2 adoption. 6-carboxyfluorescein (FAM) attachment and hybridized with its complementary unlabeled strand. Integration of fluorescent protospacers was carried out at room temperature with 250 nM Cas1, 10 nM protospacer, and 20 ng/μL plasmid for 1 hour. Reactions were quenched by
F•O•R•U•M Perspectives on Canadian Population Stationary population as a Fata Morgana

The notion of Fata Morgana has generally quite a negative connotation—it refers to a mirage, an optical illusion, and artificial, unreliable, and misleading knowledge. According to this point of view, mirages induce self-deception, and should be dispensed with as quickly as possible. There is, however, a much more positive connotation attached to an interpretation developed by Albert Hirschman (1967: 32ff). For him, human beings, especially in a world marred by deep complexity and uncertainty, have a tendency to underestimate their creativity and inner strengths in the face of those difficult circumstances. In such predicaments, simplistic utopian visions and mirages (hiding or minimizing the costs and difficulties ahead, and/or exaggerating the potential ease of overcoming them) may helpfully compensate for these infirmities of man’s imagination—in much the same way that the beautiful imaginary oasis, seen by members of a caravan deep in the desert, increases their efforts to the point that, in spite of their sufferings, they reach the next real oasis. Those of the Hirschman persuasion (like me) do not malign these sorts of êtres de raison or simplified chromos that often play a crucial role in keeping the attention on certain key issues, and imbue the exploratory drive with new energy. That is why one must be grateful to senior scholars, who, no longer feeling the need to be dans le vent, dare to work on exploratory essays built on controversial reference points, to help reframe the debates in times when most participants and observers would appear to be at a loss as to the most effective way to proceed. This sort of bold exploratory work shakes off excessive prudence in order to allow the mind not to be unduly restrained by the barnacles of traditional scholarly rules. The objective is not to build a full-fledged theory or model, but only to provide a conceptual framework—a set of relationships that may not be specific enough to lead to testable propositions about the world of events, but that provides the mold out of which specific theories are constructed by adventurous critical thinking (Leibenstein 1976: 17–18). In this Forum, Anatole Romaniuk (2017) has provided us with one such valuable exploratory essay. In such a work, it is difficult to be at the same time general, simple, and precise. Romaniuk’s advocacy paper (his words) has chosen to be general and simple. It aims at nothing less than suggesting a demographic rule—stationary population—as likely to generate optimality in all perspectives—ecological, economic, social cohesion, and national identity. Special attention is paid to Canada in this paper, but the rule is meant to be of universal application in the West. And while the author inserts, in passing, a cautious remark about this rule not being a panacea or a sufficient condition—for there may be other supplementary conditions needed to reach these optima—it is not unfair to say that this rule is meant to do much of the heavy lifting.
COMPARATIVE STUDY OF RAINFALL INFILTRATION INTO A BARE AND A GRASSED UNSATURATED EXPANSIVE SOIL SLOPE

It is generally thought that vegetation has a stabilization eŠect on a slope; however, very limited quantitative ˆeld data are available for verifying this perception. In order to improve our understanding on the vegetation eŠect on rainfall inˆltration and hence on slope stability, a well-instrumented ˆeld study was carried out on an unsaturated expansive soil slope in China. The ˆeld program consisted of two neighboring monitoring areas (both 16 m wide by about 30 m long): namely a bare area and a naturally grassed area (real slope). Artiˆcial rainfall events were produced in succession in the two areas with a specially-designed sprinkler system. In this paper, the relevant monitored results from the two areas are directly compared and discussed. Prior to the artiˆcial rainfall, the measured higher initial soil suction in the grassed area than that in the bare areas was attributed to the evapo-transpiration eŠect of the grass. During the rainfall, the presence of the grass greatly increased the inˆltrability of the upper soil layer and delayed the onset of surface runoŠ. The observed delayed responses (i.e., about 3 days) of surface runoŠ, soil suction and water content to the rainfall in the grassed area was about twice the duration of the delayed response in the bare area (i.e., about 1.5 days). The in‰uence of the simulated rainfall on the changes of soil suction and water content in the grassed area was found to be more signiˆcant and about 1.5 m deeper than that in the bare area. The greater depth of in‰uence observed in the grassed area may be attributable to the greater depth of open cracks due to the evapo-transpiration eŠect of grass.
Association of opioid receptor gene polymorphism with drinking severity and impulsivity of alcohol dependence

Background Recent evidence has suggested that endogenous opioid system is implicated in the pathophysiology of alcohol dependence. On the basis of several studies that showed associations between opioid receptor gene variants and alcohol dependence, we aimed to examine the genetic influence of opioid receptors on the susceptibility to alcohol dependence and its clinical and psychological characteristics such as multidimensional impulsivity in a Korean population. We genotyped three single nucleotide polymorphisms (SNP) (rs1799971, rs609148, rs648893) from μ-opioid receptor gene ( OPRM1 ) and two SNPs (rs702764, rs6473797) from κ-opioid receptor gene ( OPRK1 ) using the SNaPshot assay. Four SNPs were analyzed (rs1799971, rs609148, rs702764, rs6473797), because rs609148 and rs648893 were completely linked. The genotype distributions and haplotype frequencies were examined in 320 male patients with alcohol dependence and 329 age-matched male controls. To analyze the associations between opioid receptor gene variants and impulsivity in alcohol dependence, patients have undertaken the stop signal task (SST), delay discounting task (DDT), and balloon analog risk taking task (BART), which measure different aspects of impulsivity. In addition, we used several identification test
The Impact of COVID-19 on Consumer Behavior in the Luxury Industry

The following paper is aimed at understanding how the COVID-19 pandemic has changed consumers’ attitude towards consumption of luxury and discretionary goods, what are the consequences of these disruptions, and what strategies brand can employ in order to survive in a post-pandemic future. This situation served as a catalyst to accelerate some of the True Luxury Consumer trends that were beginning to gain popularity in the past few years, such as the prioritization of experiences over physical items, resulting in a tendency towards minimalism; the increased interest in sustainability, ethics, and the Corporate Social Responsibility commitments of brands; the enhanced use of online distribution channels, and the consequential need for a seamless omnichannel strategy which could effortlessly and efficiently replicate the offline, in-store experience on digital platforms; and, finally, the emergence of new ways to experience luxury goods which prioritize accessibility over ownership, such as the second-hand market for luxury as well as luxury rental services, like Rent The Runway. The pandemic has highlighted a series of issues in society, healthcare, and businesses; the consequences of these issues have been tragic, but also eye-opening for brands as well as consumers. In the clients’ perspective, big companies hold the responsibility to not only provide a product or a service, but also to be helpful assets to their communities. Brands should live up to these expectations, not just to survive in the “new normal”, but also to genuinely help build a better future once the dust settles.
Acidosomes from Dictyostelium

The acidosome, a newly described organelle in Dictyostelium discoideum, is rich in vacuolar proton pumps (V-H+-ATPases) and is responsible for the acidification of endocytic vacuoles. Purified acidosomes were not significantly contaminated by lysosomes, endosomes, or plasma membranes but contained a small fraction of contractile vacuole markers. The specific activity of the proton pump in these acidosomes reached 30 bmol/min/mg protein, the highest yet reported for any V-H+-ATPase. The V-H+-ATPase was the predominant protein in acidosomes. Based on gel electrophoresis and densitometry, its 8 polypeptides had the following apparent molecular mass (in kDa) and stoichiometry: 901, 6&, 533,421,373,253, 17e, and lbl . These values suggested a M, 8 X lo6, consistent with the hydrodynamic properties and electron microscopic image of the purified pump. The 90and 17-kDa polypeptides were integral, while the others were peripheral; only the 90kDa subunit was biosynthetically labeled by [3H]glucosamine and 36S04. The specific content of phosphatidylcholine and phosphatidylserine in the acidosomes was the highest of any subcellular fraction tested, while sterols and sphingolipids were the lowest. Acidosomes had =lo% of the lipid biosynthetically labeled with [3H]glucosamine. This organelle contributed 5% of cellular protein and 15% of the phospholipid in stationary cultures. We conclude that the acidosome in Dictyostelium is a biochemically discrete organelle, produced by the endoplasmic reticulum/Golgi apparatus but distinct from other endomembranes as well as from the plasma membrane.
Derecho de información de los pacientes: influencia de las características socioprofesionales en atención primaria

Objetivos Describir la informacion proporcionada por los medicos de atencion primaria a sus pacientes en las distintas fases del proceso asistencial y analizar si guarda relacion con las caracteristicas socioprofesionales en las que se desarrolla su labor profesional. Diseno Descriptivo, transversal. Emplazamiento Atencion primaria de Murcia. Participantes Un total de 227 medicos de atencion primaria. Mediciones principales Cuestionario que incluia: a) variables socioprofesionales (edad, sexo, estado civil, anos de ejercicio, anos en el puesto actual, medio laboral, formacion previa, numero de tarjetas sanitarias, demanda asistencial media diaria); b) valoracion de la satisfaccion profesional (mediante una escala tipo Likert) en relacion con la retribucion economica, la pertenencia al grupo profesional, la actuacion de los jefes inmediatos y la consulta diaria, y c) frecuencia en la informacion proporcionada a los pacientes sobre el diagnostico, pronostico, tratamiento, examenes complementarios e impacto socioprofesional y familiar del proceso. Resultados El porcentaje de medicos que siempre informan sobre diagnostico, tratamiento, pronostico, examenes complementarios e impacto socioprofesional y familiar del proceso fue, respectivamente, del 23,3; 7; 64,3; 40,5 y 9,7%. Hay una asociacion directa entre la satisfaccion laboral de los medicos y la informacion proporcionada a sus pacientes. La asociacion es inversa entre el numero de tarjetas sanitarias y la informacion proporcionada a los pacientes. Conclusiones El cumplimiento del deber de informacion no alcanza unos niveles de eficacia suficientes. Hay una insatisfaccion laboral generalizada en los medicos de familia que influye negativamente en el derecho de informacion del paciente. Una menor carga asistencial mejora de manera significativa la informacion proporcionada a los pacientes. Seria conveniente articular mecanismos de mejora en las condiciones laborales para evitar el incumplimiento del derecho de informacion del paciente.
A Japanese Family with Central Hypothyroidism Caused by a Novel IGSF1 Mutation

Background: Hemizygous mutations in the immunoglobulin superfamily member 1 (IGSF1) gene have been demonstrated to cause congenital central hypothyroidism in males. This study reports a family with a novel mutation in the IGSF1 gene located on the long arm of the X chromosome. Patient findings: A two-month-old boy was diagnosed with central hypothyroidism because of prolonged jaundice. A thyrotropin-releasing hormone (TRH) stimulation test indicated dysfunction in both the hypothalamus and the pituitary gland, and prompted the IGSF1 gene to be analyzed. The patient had a novel nonsense variant, c.2713C>T (p.Q905X), in exon 14 of the IGSF1 gene. Studies of the family revealed that the patient's sister and mother were heterozygous carriers of the IGSF1 mutation. The patient's maternal uncle carried the same mutation as the proband but had no overt symptoms. The mother and uncle started levothyroxine supplementation because of subclinical hypothyroidism. Summary: A novel mutation (c.2713C>T, p.Q905X) of the .
Suboptimal Dosing Parameters as Possible Factors in the Negative Phase III Clinical Trials of Progesterone for Traumatic Brain Injury

Abstract To date, outcomes for all Phase III clinical trials for traumatic brain injury (TBI) have been negative. The recent disappointing results of the Progesterone for the Treatment of Traumatic Brain Injury (ProTECT) and Study of a Neuroprotective Agent, Progesterone, in Severe Traumatic Brain Injury (SyNAPSe) Phase III trials for progesterone in TBI have triggered considerable speculation about the reasons for the negative outcomes of these two studies in particular and for those of all previous Phase III TBI clinical trials in general. Among the factors proposed to explain the ProTECT III and SyNAPSe results, the investigators themselves and others have cited: 1) the pathophysiological complexity of TBI itself; 2) issues with the quality and clinical relevance of the preclinical animal models; 3) insufficiently sensitive clinical endpoints; and 4) inappropriate clinical trial designs and strategies. This paper highlights three critical trial design factors that may have contributed substantially to .
Overexpression of Proteasome (cid:1) 5 Subunit Increases the Amount of Assembled Proteasome and Confers Ameliorated Response to Oxidative Stress and Higher Survival Rates* □ S

, The proteasome is the major cellular proteolytic ma-chinery responsible for the degradation of both normal and damaged proteins. Proteasomes play a fundamental role in retaining cellular homeostasis. Alterations of proteasome function have been recorded in various biological phenomena including aging. We have recently shown that the decrease in proteasome activity in senescent human fibroblasts relates to the down-regulation of (cid:1) -type subunits. In this study we have followed our preliminary observation by developing and further characterizing a number of different human cell lines overexpressing the (cid:1) 5 subunit. Stable overexpression of the (cid:1) 5 subunit in WI38/T and HL60 cells resulted in elevated levels of other (cid:1) -type subunits and increased levels of all three proteasome activities. Immunoprecipitation experiments have shown increased levels of assembled proteasomes in stable clones. Analysis by gel filtration has revealed that the recorded higher level of proteasome assembly is directly linked to the efficient integration of “free” (not integrated) (cid:2) -type subunits identified to accumulate in vec-tor-transfected
FavLabMaré Edition

This paper introduces and discusses the first outcome of a recently created digital fabrication laboratory at Favela da Maré, a slum in the North zone of Rio de Janeiro, Brazil. The lab called FavLab is a partnership between the João e Maria Aleixo Institute, located inside Favela da Maré, and the Department of Architecture and Urbanism of PUC-Rio University. More specifically, it aims to present the lab's first activity: a workshop devoted to create and fabricate meaningful objects to the context of favela exploiting digital design and fabrication methods. Architecture undergraduates and local young residents not enrolled in the educational system participated in the studio. This paper aims to discuss in details the experience of teaching for this particular group of students, as well as the impacts of the collaborative design between university and favela students to create interactive objects in a Brazilian community. The paper aims to reinforce and remark an innovative and inclusive approach to digital design and fabrication. This paper also attempts to discuss further developments and next steps towards more profound and broader collaboration between academia and favelas' representatives.
On the Flexure of Thin Cylindrical Shells and other “ Thin" Sections.

I t is a generally appreciated deduction from St. Venant’s solution of the flexure problem that a beam in which the material is disposed at a distance from the neutral axis is superior to the solid section in economy of material. St. Venant’s solution, however, suggests that this advantage increases without limit as the thickness of the material is reduced and the distance from the neutral axis is increased. I t has, of course, been generally realised that this conclusion is not supported by ordinary engineering practice, and recent experience in the use of high tensile steels and problems of aircraft structure have emphasised the desirability of a further examination of the flexure problem. St. Venant’s solutions are obtained when the equations of equilibrium of an isotropic elastic solid are made linear by the neglect of terms of higher orders than the first: and by Kirchhoff’s theorem of determinancy these solutions may then be considered unique and stable. To attack problems of stability it is necessary, as is shown by R. V. Southwell* in his * General Theory of Elastic Stability,’ to include some of the second order effects. I t is, in fact, only when these become considerable that Kirchhoff’s theorem fails and instability becomes possible. By this general treatment various classes of instability are obtained or indicated, but the only ones susceptible to analysis or of practical interest (on account of the “ elastic limit ” which is a feature of all practical materials) are those in which at the moment of instability the strains are still small. Bryantf has shown that this will only occur, as in the case of thin rods and shells, when one dimension of the body is small compared with others. In a similar way we may expect that when one dimension of the cross section of the body is small compared with others it will be necessary to include second order terms in problems purely of flexural equilibrium. This is really evident in the case of the flexure problem from St. Venant’s explicit description of the stress at any element of the cross section as a function of the initial position of the element. The longitudinal stress, for example, is prescribed as directly proportional to the initial distance of the element from the line of centroids or
Schistosoma mansoni infection reprograms the metabolic potential of the myeloid lineage in 1 a mouse model of metabolic syndrome 2 3 4

1 Department of Pathology, Division of Microbiology and Immunology, 8 University of Utah, Salt Lake City UT, 84112 9 2 Bindley Bioscience Center, Purdue University, West Lafayette IN, 47907 10 3 Department of Biochemistry, University of Utah, Salt Lake City UT, 84112 11 4 Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405 12 5Metabolomics, Proteomics and Mass Spectrometry Cores, University of Utah, Salt Lake City, UT, 13 84112 14 6 Department of Nutrition and Integrative Physiology and the Diabetes and Metabolism 15 Research Center, University of Utah, Salt Lake City, UT 84112 16 17 18 Running title: Schistosomes reprogram myeloid metabolism 19 20 21 7Lead contact: 22
and Competitive

The competitive in global market requires a continuous transformation in information technology contents, mainly by the insertion of new tools and techniques that rapidly evolve and are absorbed by members of the organizations. This advanced of new technologies in organizations scenario brought challenges addressed to need to collect, store, process and transform their data into relevant information to create competitive advantage. In highly dynamic business environments, the possibility of generating information pushes companies to use efficiently a huge volume of data, in both resources and capacities approaches. This paper aimed to analyze the relation between Big Data and competitive advantage using the resource-based view and the dynamic capabilities. Three international databases were used and analyzed 29 articles. Results indicated that both the resource-based view and the dynamic capabilities approach are used to analyze Big Data as competitive advantage creator. Two aspects were evidenced: the combined approaches can result in more consistent analyses; and the lack of a robust theoretical framework for Big Data analysis is evidenced by the predominance of descriptive articles and practical reports.