Miracle-dz/UniFAR-FTUs huggingface dataset:
Alternative Mechanisms for Coordinating Polymerase (cid:1) and MCM Helicase (cid:1)

Functional coordination between DNA replication helicases and DNA polymerases at replication forks, achieved through physical linkages, has been demonstrated in prokaryotes but not in eukaryotes. In Saccharomyces cerevisiae , we showed that mutations that compromise the activity of the MCM helicase enhance the physical stability of DNA polymerase (cid:1) in the absence of their presumed linker, Mcm10. Mcm10 is an essential DNA replication protein implicated in the stable assembly of the replisome by virtue of its interaction with the MCM2-7 helicase and Pol (cid:1) . Dominant mcm2 suppressors of mcm10 mutants restore viability by restoring the stability of Pol (cid:1) without restoring the stability of Mcm10, in a Mec1-dependent manner. In this process, the single-stranded DNA accumulation observed in the mcm10 mutant is suppressed. The activities of key checkpoint regulators known to be important for replication fork stabilization contribute to the efﬁciency of suppression. These results suggest that Mcm10 plays two important roles as a linker of the MCM helicase and Pol (cid:1) at the elongating replication fork—ﬁrst, to coordinate the activities of these two molecular motors, and second, to ensure their physical stability and the integrity of the replication fork. was cloned into the plasmid and mutagenized in Escherichia by using XL1-red competent (Stratagene). Mutagenized plasmids were obtained from E. coli , transformed into mcm10 - , and plated at 37°C to select for suppressors. The plasmids were sequenced to determine the nature of the mutations. and puriﬁcation of mutant proteins. Methanothermobacter Extraction of phosphorylated Rad53 also contained phosphatase inhibitors. The mild- alkali-treatment (0.2 M NaOH) method produced a protein extraction yield similar to that of glass bead lysis. Mouse anti-myc (Santa Cruz) and mouse anti-hemagglutinin (HA; Roche) antibodies were used to probe for the appropriate myc-tagged and HA-tagged proteins. Goat anti-mouse horseradish per- oxidase-conjugated secondary antibodies were obtained from Bio-Rad. Blots were visualized by chemiluminescence reagents (Promega). FACS. For overall ratio of cells in G 1 , S, or G 2 phase, log-phase cells of wild-type, mcm10-1 , mcm2-G400D and mcm10 mcm2 strains were collected without (cid:1) -factor arrest. For cell cycle progression, log-phase cultures were arrested in G 1 phase by (cid:1) -factor for 2 h. Cells were spun down and resuspended in fresh yeast extract-peptone-dextrose (YPD) media containing 100 (cid:6) g/ml of pro-nase (Sigma) for rapid (cid:1) -factor degradation and release into S phase. The G 1 -arrested cells were released at either 30°C or 37°C. For the latter, the cells were preincubated at 37°C for 1 h before release to allow Mcm10 degradation to occur before the onset of S phase. Samples at different time points were collected for ﬂuorescence-activated cell sorting (FACS) analysis at the Cornell FACS facility. Visualization yellow ﬂuorescent (YFP)-conjugated RPA was carried out in under conventional ﬂuorescence (cid:3) charge-coupled-device (Improvision).
AAST 2016 PLENARY PAPER Hospitals with higher volumes of emergency general surgery patients achieve lower mortality rates: A case for establishing designated centers for emergency general surgery

Submitted: August 22, 2016, Revised: November 16, 2016, Accepted: December 6, 2016, Published online: December 28, 2016. From the Center for Clinical Effectiveness, Office of the Chief Quality Officer (G.O.G., S.S.), Baylor Scott & White Health, Dallas, TX; and Center for Surgery and Public Health, Department of Surgery (A.H.), Brigham and Women’s Hospital, Boston, MA. This study was presented at the75th annual meeting of the American Association for the Surgery of Trauma, September 14–17, 2016, in Waikoloa, Hawaii. Address for reprints: Shahid Shafi, MD, MPH, FACS, 8080 North Central Expressway, Suite 500, Dallas, TX 75026; email: shahid.shafi@bswhealth.org.
Effect of Time and Angle Etching in the Performance Heterojunction Si Solar Cells

In this paper, Glancing Angle Deposition Technique (GLAD) has been used to grow Silicon (Si) thin film by vacuum thermal method on glass substrates. Heterojunction with Intrinsic Thin-Layer (HIT) solar cells are sensitive to interface state density. Traditional texture process for silicon solar cells is not suitable for HIT one. Thus, Sodium Hydroxide (NaOH), Isopropanol (IPA) and mixed additive were tentatively introduced for the texturization of HIT solar cells in this study. Then, a mixture including nitric acid (HNO 3 ), Hydrofluoric Acid (HF). The morphology of textured surface and the influence of etching time on surface reflectance were studied, and the relationship between etching time and surface reflectance, vertex angle of pyramid structure was analyzed. It was found that the mixture consisting of 1.1 wt% NaOH, 3 vol% IPA and 0.3 vol% additives with etching time of 60min is the best for HIT solar cells under the condition of 80 °C. By used SEM and optical properties the average surface reflectance was 11.68%. Finally the effect of different processes on the performance of HIT solar cells was investigated. Films deposited at large angles (00, 100, 200, 300, 450, 600) was found to be poly-crystalline in nature along the peak intensity direction. The surface morphology has shown an improvement without the presence of secondary phases for higher incident angles (45°). It has been observed that the use of this growth technique leads to an improvement in the optical properties of the films.
Funds For Startups In Vietnam

In the present age, startup phenomenon which is new with authors as well as investors is increasingly notable because of its nature and contribution for the nation’s economy. A lot of theoretical researches and experimental tools have been triggered to understand and foster the lives of new ventures. Among resources that startups need to survive, the important of money resource is undeniable. However, almost small businesses have a shortage of it and are difficult to reach the fulfillment of financing methods. The reasons for this are they are new and get a lot of risks which hardly meets the criterion of traditional investors. To solve the gap of fund for startups, some new financing methods are suitable and approachable, crowdfunding is one of them. This paper aims two goals, firstly, describing the increase of that new method as well as the role of it to new ventures all over the world and in Vietnam, secondly, suggesting some solution direction to improve the effectiveness of crowdfunding activities in Vietnam.
Wireless location estimation using generalized linear models

We propose maximum likelihood (ML) methods for location estimation using received-signalstrength (RSS) and time-of-arrival (TOA) measurements. In the RSS case, we utilize both spatial and temporal measurements where the multipath fading effects are modeled using a Nakagami-m (gamma) fading model. We also propose to model the TOA measurement errors using the gamma distribution characteristic. We also derive Cramer-Rao bound (CRB) for the estimated location under both measurement scenarios. Simulation results show that the proposed algorithms outperform the commonly-used least-squares methods. CHAPTER
Mass Innovation-a competitive strategy for High-Tech B2B SMEs

II TABLE OF CONTENT III LIST OF FIGURES V LIST OF TABLES VI 1 INTRODUCTION 1 1.1 OBJECTIVES 3 1.2 STRUCTURE OF THE THESIS 4 2 INNOVATION -­‐ THEORETICAL OVERVIEW 5 2.1 DEFINITION AND TAXONOMY OF INNOVATION 5 2.1.1 INCREMENTAL VERSUS RADICAL INNOVATION 7 2.1.2 SUSTAINING VERSUS DISRUPTIVE INNOVATIONS 8 2.1. 3 CRITICAL REMARKS 10 2.2 SOURCES OF INNOVATION 12 2.2.1 THE CLOSED INNOVATION PARADIGM 13 2.2.2 OPEN INNOVATION & CUSTOMER CO-­‐CREATION 14 2.2.3 APPLYING OPEN INNOVATION 17 2.2.4 CRITICAL REMARKS ON OPEN INNOVATION 21 2.3 IS INNOVATION PER SE A PRODUCT? 22 2.4 DIFFUSION OF INNOVATION 23 3 MASS INNOVATION – THEORETICAL CONCEPT 25 3.1 VISIONARIES VS. PRAGMATICS: THE CHASM IN TALC 25 3.1.1 CRITICAL REMARKS TO TALC 27 3.2 THE LONG TAIL 28 3.3 MASS CUSTOMIZATION 30 3.3.1 DEFINITION AND CONCEPTS 30 3.3.2 APPROACHES TO MASS CUSTOMIZATION 33 3.3.3 MC AND POSTPONEMENT: LEAGILITY 34 3.3.4 MODULAR PRODUCT ARCHITECTURE 36 3.3.5 MASS CUSTOMIZATION GENERATED VALUE 37 3.3. 6 PREREQUISITES FOR MASS CUSTOMIZATION 40 IV 3.4 DUALITY AS STRATEGIC ORGANIZATIONAL MANAGEMENT 41 3.4.1 DUALITY RELATIONSHIPS 43 3.4.2 REMARKS ON DUALITY 45 3.5 MASS INNOVATION 46 4 PHOTON CASE: HIGH-­‐TECH B2B SME FACING CHASM 50 4.1 PHOTON: COMPANY PROFILE 50 4.2 THE MARKET 51 4.2.1 THE END CUSTOMER 51 4.2.2 COMPETITION 54 4.2.3 MARKET TRENDS 55 4.2.4 PHOTON’S MARKET POSITION 56 4.3 PHOTON’S BUSINESS MODEL 57 4.3.1 SELLING INNOVATION 57 4.3.2 THE PRODUCT MIX 58 4.4 MASS INNOVATION AT PHOTON 60 4.4.1 OPEN INNOVATION 60 4.4.2 MASS CUSTOMIZATION 61 4.4.3 STABILITY-­‐CHANGE DUALITY 62 4.4.4 MASS INNOVATION– PHOTON’S VEHICLE TO CROSS THE CHASM 64 5 SUMMARY AND CONCLUSION 65 5.1 SUMMARY 65 5.2 LIMITATIONS 66 5.3 CONCLUSIONS 66 6 BIBLIOGRAPHY 67
Percutaneous Mitral Valve Repair to Treat Secondary Mitral Regurgitation in Dilated Cardiomyopathy: Are the Hopes Greater than the Achievements? EC Cardiology Editor’s Column - 2019

Mitral regurgitation may be primary when there is leaflet abnormality, whereas it is secondary to ischemic or dilated cardiomyopathy when there is a structurally normal valve leaflets that are unable to coapt completely because of damaged of the supporting mitral valve apparatus. There is a controversial agreement on how to manage these two types of mitral regurgitation. Current guidelines recommend early surgery for severe primary mitral regurgitation [1-3]. However, there are confronted opinions on the therapeutic management of secondary mitral regurgitation. Heart failure (HF) patients who have moderate to severe secondary mitral regurgitation due to left ventricular dysfunction and dilatation have a worse prognosis than those HF patients who have left ventricular dysfunction but without mitral regurgitation [4,5]. It still remains unclear if mitral regurgitation is simply a marker of a diseased left ventricle or if it is a causative factor that contributes to left ventricular dilatation and progression of volume overload leading to poor clinical outcomes [6]. Optimal medical treatment for cardiomyopathy, as well as, cardiac resynchronization therapy can reduce the severity of secondary mitral regurgitation and are currently the only management strategies with a class I recommendation in recent clinical guidelines [2,3]. On the other hand, cardiac surgery for secondary mitral regurgitation has not been tested in clinical trials and there is no evidence for a clear benefit [7,8].
Nebivolol The Somewhat-Different (cid:1) -Adrenergic Receptor Blocker

Although its clinical use in Europe dates almost 10 years, nebivolol is a (cid:1) -blocker that has been only recently introduced in the U.S. market. Like carvedilol, nebivolol belongs to the third generation of (cid:1) -blockers, which possess direct vasodilator properties in addition to their adrenergic blocking characteristics. Nebivolol has the highest (cid:1) 1 -receptor afﬁnity among (cid:1) -blockers and, most interestingly, it substantially improves endothelial dysfunction via its strong stimulatory effects on the activity of the endothelial nitric oxide synthase and via its antioxidative properties. Because impaired endothelial activity is attributed a major causal role in the pathophysiology of hypertension, coronary artery disease, and congestive heart failure, the endothelium-agonistic properties of nebivolol suggest that this drug might provide additional beneﬁt beyond (cid:1) -receptor blockade. Although lesser (cid:1) -blocker–related side effects have been reported in patients with chronic obstructive pulmonary disease or impotence taking nebivolol, side effects and contraindications overlap those of other (cid:1) -blockers. Clinically, this compound has been proven to have antihypertensive and anti-ischemic effects as well as beneﬁcial effects on hemodynamics and prognosis in patients with chronic congestive heart failure. Further studies are now necessary to compare the beneﬁt of nebivolol with that of other drugs in the same class and, most importantly, its prognostic impact in patients with hypertension. (J Am Coll Cardiol 2009;54:1491–9) © 2009 by the American College of Cardiology Foundation
Hidden-order pseudogap in URu2Si2

Through an analysis and modeling of data from various experimental techniques, we present clear evidence for the presence of a hidden-order pseudogap in URu${}_{2}$Si${}_{2}$ in the temperature range 25\char21{}17.5 K. Considering fluctuations of the hidden-order energy gap at the transition, we evaluate the effects that gap fluctuations would produce on observables like tunneling conductance, neutron scattering, and nuclear resonance, and relate them to the experimental findings. We show that the transition into the hidden-order phase is likely second order and is preceded by the onset of noncoherent hidden-order fluctuations.
Biological Control of Onion White Rot Disease using Bacillus spp. Isolated from Egyptian Soil

he antagonistic ability of eleven bacterial isolates against Sclerotium cepivorum, causal agent of onion white rot disease (OWR), was determined in vitro and the best five isolates were identified as Bacillus pumilus (BP 1 and BP 2 ) and B. marinus (BM 1 , BM 2 and BM 3 ). The in vitro antagonistic efficacy ranged between 11.1 to 68.9% depending on the concentration of the bacterial cell suspension and number of applied sprays under greenhouse and field conditions. It was found that using double sprays with B. pumilus (BP 2 ) in the greenhouse experiment gave the highest efficacy in reducing OWR (93.8%) at the concentrations of 10 and 20 ml/l. In case of field experiment there was no significant deference between number of sprays or the tested bacteria in reducing infection percentage of OWR. The significant difference was reported only between the concentrations of the bacterial suspension during the two growing seasons 2008/2009 – 2009/2010. In case of using one spray during season 2008/2009, the most effective bacteria in reducing OWR was B. pumilus (BP 2 ) giving 64.7 and 61.6% efficacy when used at the concentrations 20ml/l and 10 ml/l, respectively, followed by B. marinus (BM 3 ) which gave 58.2% at the concentration 20ml/l. On the other hand, when two sprays of bacterial suspension were used B. marinus (BM 1 ) and B. pumilus (BP 1 ) gave the highest efficacy in reducing OWR reached 63.7 and 63.4%, respectively. During season 2009/2010 B. pumilus (BP 2 ) was the most effective bacteria reached 80.0% efficacy followed by B. marinus (BM 3 ) recorded 76.9% when both used at the concentration 20ml/l for one spray. When two sprays were used, B. marinus (BM 2 ) gave the highest percentage of efficacy in reducing OWR (87.3%). Data showed that applying the tested bacteria at any concentration, either once or twice, significantly increased onion bulb yield compared with control treatment during the two growing seasons.
Dietary docosahexaenoic acid supplementation modulates hippocampal development in the Pemt -/- mouse

The development of fetal brain is influenced by nutrients such as docosahexaenoic acid (DHA, 22:6) and choline. Phosphatidylethanolamine-Nmethyltransferase (PEMT) catalyzes the biosynthesis of phosphatidylcholine (PtdCho) from phosphatidylethanolamine that is enriched in DHA and many humans have functional genetic polymorphisms in the PEMT gene. Previously, it was reported that Pemt-/mice have altered hippocampal development. The present study explores whether abnormal PtdCho biosynthesis causes altered incorporation of DHA into membranes, thereby influencing brain development, and determines whether supplemental dietary DHA can reverse some of these changes. Pregnant C57BL/6 wild type (WT) and Pemt-/mice were fed control diet, or diet supplemented with 3g/kg DHA, from gestational day 11-17. Brains from embryonic day 17 fetuses derived from Pemt-/dams fed the control diet had 2550% less phospholipid-DHA as compared to WT (p<0.05). Also, they had 60% more neural progenitor cell proliferation (p<0.05), 60% more neuronal apoptosis (p<0.01) and 30% less calretinin expression (p<0.05; a marker of neuronal differentiation) in the hippocampus compared to WT. The DHA supplemented diet increased fetal brain Pemt-/phospholipid-DHA to WT levels, and abrogated the neural progenitor cell proliferation and apoptosis differences. While this diet did not change proliferation in the WT group, it halved the rate of apoptosis (p<0.05). In both genotypes, the DHA supplemented diet increased calretinin expression 2-fold (p<0.05). These results suggest that the changes in hippocampal development in the Pemt-/mouse could be mediated by altered DHA incorporation into membrane phospholipids, and that maternal dietary of DHA can influence fetal brain development.
1 Audio adaptation for mobile listening

Mobile devices enable users to consume media with audio content in a wide range of contexts, with environmental noise being present in many of these. Several methods exist that aim to improve the experience of mobile listening by utilising information about the environmental noise, such as volume and dynamic range adaptation. This paper explores a fundamentally different approach to improving the mobile listening experience by using the object-based audio paradigm, where individual audio sources are mixed in response to each specific listening context. Three experimental studies, containing both quantitative and qualitative aspects, are presented which investigate whether environmental noise influences preference of background-foreground audio object balance in a mix. The results indicate that environmental noise can influence the preferred audio mix and that the nature of the adaptations made is dependent upon both audio content and user. Additionally, qualitative analysis provides an understanding of the role of environmental noise on preferred audio mix. It is believed that the content adaptation method explored in this paper is a simple yet useful tool for adapting content to suit both the context and the user.
-Fluoro-Modified Pyrimidines Enhance Affinity of RNA Oligonucleotides to HIV-1 Reverse Transcriptase

Nucleic acid aptamers can be chemically modified to enhance function, but modifying previously selected aptamers can have non-trivial structural and functional consequences. We present a reselection strategy to evaluate the impact of several modifications on pre-existing aptamer pools. RNA aptamer libraries with affinity to HIV-1 reverse transcriptase (RT) were re-transcribed with 2 and subjected to three additional selection cycles. RT inhibition was observed for representative aptamers from several structural families identified by high-throughput sequencing when transcribed with their corresponding modifications. Thus, reselection identified specialized subsets of aptamers that tolerated chemical modifications from unmodified pre-enriched libraries. Inhibition was the strongest with the 2 -F-pyrimidine (2 ′ -FY) RNAs, as compared to inhibition by the 2 -OMeY and 2 -NH 2 Y RNAs. Unexpectedly, a diverse panel of retroviral RTs were strongly inhibited by all 2 ′ -FY-modified transcripts, including sequences that do not inhibit those RTs as unmodified RNA. The magnitude of promiscuous RT inhibition was proportional to mole fraction 2 ′ -FY in the transcript. RT binding by 2 ′ -FY transcripts was more sensitive to salt concentration than binding by unmodified transcripts, indicating that interaction with retroviral RTs is more ionic in character for 2 ′ -FY RNA than for unmodified 2 ′ -OH RNA. These surprising features of 2 ′ -FY-modified RNA may have general implications for applied aptamer technologies.
ISOLATION AND SEROTYPING OF SALMONELLA SPECIES FROM RAW MILK OF COWS, BUFFALOES AND SHEEP IN ASSIUT GOVERNORATE

salmonella Spp. could be detected from raw milk of different animals were 25 (6.17%) positive samples, 1 (2%), 10 (20%) and 14 (28%) for cows, buffaloes and sheep milk, respectively. Serotyping of the isolated salmonella spp. revealed that, the Salmonella gallinarum was the most prevalent species among the salmonalla spp. 15 isolates (10%) 10 (20%) from buffaloes and 5 (10%) from sheep. Salmonella enteritidis was isolated from 9 samples, 1 (2%) from cows and 8 (16%) from sheep but only one sample of sheep milk was positive for salmonella typhi. All types of the isolated salmonella were highly sensitive to Norofloxacin of the tested antiblotics and moderately sensitive to streptomycin and weekly sensitive to Gentamycin, Rifampin, Ampicillin, Cefotaxime, cefadroxil and chloramphenicol. However salmonella gallinerum was moderately sensitive to Gentamycin and resistant to Cefadroxil and Chloramphenical.
VITAMIN D DEFICIENCY IN PATIENTS WITH THYROID DISORDER

: OBJECTIVE: To determine the vitamin D deficiency in patients with thyroid disorder. STUDY DESIGN AND SETTING: This was a cross-sectional study and carried out ant medicine department of Indus medical college Tando Muhammad khan. Study duration was 6 month from December 2016 July 2017. MATERIAL AND METHODS: All the thyroid disorder patients, either gender were studied. All the patients with thyroid carcinoma, diabetes, lactation and known cases of chronic hepatitis excluded from study. 5ml blood was taken from each patient for assessment of vitamin D level. All the data regarding age, gender, hypothyroidism and hyperthyroidism was entered in proforma. RESULTS: Total 67 patients of thyroid disorder were selected, their mean age was 39.75+5.61 years. Female gender was most common as 62.7%. A positive correlation was found between vitamin D level and TSH r-value = 0.015. Total 43.3% thyroid disorder patients were found with vitamin D deficiency, insufficiency was 33.3% and 25.4% patients were with normal vitamin D level. No significant association was found in vitamin D efficiency according to hypothyroidism and hyperthyroidism p-value 0.992. CONCLUSION: It is concluded that vitamin D deficiency was 43.3% and insufficiency was 31.3% and positive correlation was found between vitamin D level and TSH. The pleiotropic roles of vitamin D have been recognized through preclinical and observational studies which have suggested a beneficial role of vitamin D in the management of thyroid disease.
Acid Ceramidase in Melanoma EXPRESSION, LOCALIZATION, AND EFFECTS OF PHARMACOLOGICAL INHIBITION

Acid ceramidase (AC) is a lysosomal cysteine amidase that controls sphingolipid signaling by lowering the levels of ceramides and concomitantly increasing those of sphingosine and its bioactive metabolite, sphingosine 1-phosphate. In the present study, we evaluated the role of AC-regulated sphingolipid signaling in melanoma. We found that AC expression is markedly elevated in normal human melanocytes and proliferative melanoma cell lines, compared with other skin cells (keratino-cytes and fibroblasts) and non-melanoma cancer cells. High AC expression was also observed in biopsies from human subjects with Stage II melanoma. Immunofluorescence studies revealed that the subcellular localization of AC differs between melanocytes (where it is found in both cytosol and nucleus) and melanoma cells (where it is primarily localized to cytosol). In addi-tion to having high AC levels, melanoma cells generate lower amounts of ceramides than normal melanocytes do. This down-regulation in ceramide sup-pression of the de novo biosynthesis pathway. To test whether ACmightcontributetomelanomacellproliferation,weblocked AC activity using a new potent (IC 50 (cid:2) 12 n M ) and stable inhibitor. AC inhibition increased cellular ceramide levels, decreased sphingosine 1-phosphate levels, and acted synergistically with several, albeit not all, antitumoral agents. The results suggest that AC-controlled sphingolipid metabolism may play an important role in the control of melanoma proliferation.