Lowering Blood Cholesterol Levels Resulted from Sea-Shrimp's Shell Supple- mentation (Palaemon Sp) in Rats Fed on Pig Oil

Background: Hypercholesterolemia is the most important risk factor for coronary heart disease. Chitosan dietary supplementations demonstrate that the cholesterol decreases after binding lipid and cholesterol and inhibiting their absorption. Effect of CUL to lower cholesterol levels is still unclear. The aim of the present study was to prove that total cholesterol (CT), Low Density Lipoprotein (LDLc), Triglyceride (TG), and increasing of High Density Lipoprotein (HDLc) level are resulted from CUL supplementation which is given with pig oil simultaneously. Design and Method: Twenty seven rats (200 gr) were included in a Post Test Control Group Design study. The rats wererandomly divided into 3 groups of 9 rats each . Group A was as a control, group B was treated with 15% CUL supplementation, and group C was treated with 30% CUL supplementation. A week after rat’s acclimatization, the CUL supplementation was given for 45 consecutive days in ad libitum manner. Measurement of the CT, LDLc, HDLc, and TG levels were undertaken at the final day of CUL supplementation. Results: The present study demonstrated that CT, LDLc, and TG levels in CUL 15% and 30% supplementation ware significantly lower (p = 0.000) compared to that of un-supplemented group. Meanwhile HDLc levels underwent significant elevation (p = 0.000) compared to the un-supplemented group. The discriminant analysis showed that CT and LDLc were the discriminant variables among four dependent variables. This result suggested that CT and LDLc variables having a direct correlation were resulted from CUL supplementation. Thus, elevation of CT levels was followed by the increase in LDLc levels. On the other hand lowering of CT was followed by decrease in LDLc. Because of univariate analysis upon HDLc and TG level resulted in significant difference, so elevation of HDLc and decrease in TG remain the importance parameters for dyslipidemia. Conclusion: Lowering of CT, LDLc, TG and enhancing HDLc level are resulted from supplementation with CUL 15% and 30% for 45 consecutive days in rats has been proven (Sains Medika, 2(2):134-150). Pendahuluan: Hiperkolesterolemia merupakan faktor risiko yang sangat penting untuk PJK. Suplementasi chitosan mampu menurunkan kadar kolesterol setelah mengikat dan menghambat absorbsi lemak dan kolesterol. Efek cangkang udang laut (CUL) untuk menurunkan kadar kolesterol masih belum jelas. Penelitian ini bertujuan untuk membuktikan penurunan kadar kolesterol total (CT), Low Density Lipoprotein (LDLc), Trigliserid (TG), dan peningkatan kadar High Density Lipoprotein (HDLc) akibat suplementasi CUL yang diberikan bersama dengan minyak babi. Metode Penelitian: Dua puluh tujuh ekor tikus umur 60 hari dengan berat badan (BB) + 200 gram dimasukkan sebagai sampel dalam penelitian Post Test Control Group Design. Tikus dibagi menjadi 3 group masing-masing terdiri dari 9 ekor. Group A sebagai kontrol, group B suplementasi CUL 15%, dan group C suplementasi CUL 30%. Setelah satu minggu aklimatisasi tikus diberi pakan sesuai groupnya secara ad libitum selama 45 hari berturut-turut. Pengukuran kadar CT, LDLc, HDLc, dan TG dilakukan di akhir suplementasi CUL. Hasil: Penelitian ini menunjukkan bahwa kadar CT, LDLc, dan TG pada suplementasi CUL 15% dan 30% lebih rendah secara bermakna (p = 0.000) dibanding tanpa suplementasi. Di sisi lain kadar HDLc