Miracle-dz/UniFAR-FTUs huggingface dataset:
Nanostructured conducting molecularly imprinted polypyrrole based quartz crystal microbalance sensor for naproxen determination and its electrochemical impedance study

This paper represents a naproxen (NAP) imprinted nanostructured conducting molecularly imprinted polypyrrole (CMIP) film which is electrochemically synthesized on a quartz crystal microbalance (QCM) gold electrode and electrochemically overoxidized for increasing its selectivity as a recognition element of a QCM sensor for the detection of NAP. It was shown that the selectivity of the obtained film toward NAP was enhanced by controlled overoxidation. The characteristics of CMIP and overoxidized (OCMIP) films were investigated by ATR FT-IR, cyclic voltammetry and electrochemical impedance spectroscopy (EIS), and the morphology and coverage of the film were studied by FE-SEM. Parameters of cyclic voltammetric preparation of the CMIP film including the potential range, scan rate, number of cycles (film thickness) and the effect of surfactant on polymer film growth were optimized. The overoxidation condition including overoxidation potential, number of cycles and pH and type of electrolyte solution were studied and optimized. It was found that the excessive overoxidation of CMIP film would cause two unwanted effects on the sensor; first the loss of sensor regeneration in repeated uses, and second, the decrease of the cohesion of film on the gold electrode. Thus the amount of overoxidation of the film was controlled very carefully to obtain the best selectivity with minor loss of film cohesion and sensor reproducibility. This aim was satisfied by the aid of impedimetric study of the film before and during the overoxidation. Results obtained from the QCM and impedance measurements show that overoxidized NAP imprinted CMIP could be used for determination of NAP in aqueous media with a limit of detection of 0.04 μmol L−1 and RSD = 4.9% (n = 5). The proposed sensor offers a good selectivity and cost effective properties for fast determination of NAP with ease of preparation and simple instrumentation.
Effects of Fire on Ground-Dwelling Spider (Araneae) Assemblages in Central Indiana Forests

Abstract Fire is a natural disturbance that occurs in many temperate and tropical ecosystems worldwide. As ubiquitous members of these ecosystems, spiders (Araneae) are often affected by fire, and their response to this disturbance has been shown to be dependent on taxonomy, functional diversity, seasonality, and a variety of environmental factors. We examined the effect of fire on ground-dwelling spider assemblages in temperate forests in central Indiana over 5 yr and found that spider assemblages were significantly affected by fire disturbance. Overall spider abundance decreased, whereas species richness and diversity remained unaffected. We also found that spider response depended heavily on the family and/or guild to which the spider belonged. We suspect that altered habitat heterogeneity, the patchy nature of fire's effect on leaf litter, and the high rate of recolonization by spiders all played important roles in these observed patterns.
Compliance Assessment of the Epithelial or Absorbed Power Density Below 10 GHz Using SAR Measurement Systems

The introduction of new dosimetric quantities, in particular, epithelial or absorbed power density for frequencies above 6 GHz, in exposure guidelines and safety standards requires the development of new experimental assessment procedures for compliance testing. In this study, we propose to approximate the peak spatial‐average absorbed power density (psSab) using the same measured data and algorithms that are used for determining the peak spatial‐average specific absorption rate psSAR, which is currently limited to frequencies up to 10 GHz. The uncertainty component for the transformation of psSAR to psSab was evaluated as less than 0.55 dB (13.5%) for any source as close as 0.02 λ from the tissue simulating media. The approach is easy to implement and allows determining compliance with the basic restrictions of the latest safety guidelines. In the next project, we will expand dosimetric probes, phantoms, and procedures for frequencies above 10 GHz. © 2021 Bioelectromagnetics Society.
Association between smoke-free legislation in hospitality venues and smoking behavior of young people: a systematic review.

INTRODUCTION While evaluations of indoor smoke-free legislation have demonstrated major public health benefits among adults, their impact on smoking behavior of young people remains unclear. Therefore, we performed a systematic review of the association between smoke-free legislation in hospitality venues and smoking behavior of young people. METHODS A systematic search was conducted in PubMed, Scopus, and Embase in June 2020. We searched for studies that assessed the association of any form of smoke-free legislation in any hospitality venue (e.g. bar, restaurant) with a smoking behavior outcome (e.g. initiation, current smoking) among young people (aged 10-24 years). RESULTS Our search yielded 572 articles of which 31 were screened based on full-text and 9 were included in the analysis. All studies were published between 2005 and 2016. The majority of studies used a quasi-experimental design. Four studies evaluated smoke-free legislation in hospitality venues specifically. Two studies reported that comprehensive, but not weaker, smoke-free legislation decrease progression to established smoking. Two other studies provided mixed results on which level of comprehensiveness of legislation would be effective, and which smoking outcomes would be affected. Five studies evaluated legislation that also included other workplaces. Out of these five studies, three studies found significant decreases in current smoking, smoking frequency, and/or smoking quantity, while two other studies did not. CONCLUSION Most studies found that smoke-free legislation in hospitality venues is associated with a decrease in smoking behavior among young people. Their results indicate the need for comprehensive smoke-free legislation without exemptions. IMPLICATIONS This is the first systematic review to provide insight in the relationship between smoke-free legislation in hospitality venues and smoking behavior of young people. Our findings show that there is a need for comprehensive smoke-free legislation without exemptions (such as designated smoking areas).
Autosomal dominant variants in FOXJ1 causing primary ciliary dyskinesia in two patients with obstructive hydrocephalus

Primary ciliary dyskinesia (PCD) is a mostly autosomal recessive, genetic disease of abnormal motile cilia function, resulting in bronchiectasis, infertility, organ laterality defects, and chronic otolaryngology disease. Though motile, ependymal cilia influencing cerebrospinal fluid flow in the central nervous system share many aspects of structure and function with motile cilia in the respiratory tract, hydrocephalus is rarely associated with PCD. Recently, pathogenic variants in FOXJ1 (Chr 17q25.1) were identified causing PCD associated with hydrocephalus, reduced respiratory cilia number, axonemal microtubule disorganization, and occurring in a de novo, autosomal dominant inheritance pattern.
Tooth loss is associated with an increased risk of hypertension: A nationwide population-based cohort study

Tooth loss is closely associated with suboptimal oral care. Suboptimal oral care can facilitate local infections. These can lead to systemic inflammation and endothelial dysfunction, which are important pathological mechanisms of hypertension. The aim of this study was to investigate the link between tooth loss and the risk of hypertension. From the national health insurance system-health screening cohort in Korea, 19,680 participants who underwent three or more health examinations, including blood pressure measurements, between January 2003 and December 2008, without any history or diagnosis of hypertension were included in this study. Hypertension was defined as the diagnosis of hypertension (International Classification of Diseases-10 code “I10–11”) accompanied by the prescription of an antihypertensive agent or at least one health examination result of blood pressure of ≥140/90 mmHg. Kaplan-Meier survival curves with the log-rank test were used to evaluate the relationship between oral hygiene indicators and the incidence of hypertension. Cox proportional hazard models were applied to determine the association between oral hygiene indicators and the development of hypertension. During a median follow-up of 7.4 years, 1,853 patients developed hypertension. The estimated incidence of hypertension within seven years was 8.8%. Multivariable analysis confirmed a significant relationship between the number of lost teeth and hypertension (hazard ratio: 2.26; 95% confidence interval [1.24–4.10], p = 0.007, p for trend = 0.005). There was a positive association between the number of lost teeth and the risk of hypertension in a longitudinal research. In conclusion, the number of lost teeth may be associated with the risk of development of hypertension.
Anisotropic compliance of robot legs improves recovery from swing-phase collisions

Uneven terrain in natural environments challenges legged locomotion by inducing instability and causing limb collisions. During the swing phase, the limb releases from the ground and arcs forward to target a secure next foothold. In natural environments leg–obstacle collisions may occur during the swing phase which can result in instability, and may require contact sensing and trajectory re-planning if a collision occurs. However, collision detection and response often requires computationally- and temporally-expensive control strategies. Inspired by low stiffness limbs that can pass past obstacles in small insects and running birds, we investigated a passive method for overcoming swing-collisions. We implemented virtual compliance control in a robot leg that allowed us to systematically vary the limb stiffness and ultimately its response to collisions with obstacles in the environment. In addition to applying a standard positional control during swing motion, we developed two virtual compliance methods: (1) an isotropic compliance for which perturbations in the x and y directions generated the same stiffness response, and (2) a vertical anisotropic compliance in which a decrease of the upward y vertical limb stiffness enabled the leg to move upwards more freely. The virtual compliance methods slightly increased variability along the limb’s planned pathway, but the anisotropic compliance control improved the successful negotiation of step obstacles by over 70% compared to isotropic compliance and positional control methods. We confirmed these findings in simulation and using a self-propelling bipedal robot walking along a linear rail over bumpy terrain. While the importance of limb compliance for stance interactions have been known, our results highlight how limb compliance in the swing-phase can enhance walking performance in naturalistic environments.
Pomegranate peel as a phytogenic in broiler chickens: Influence upon antioxidant, lipogenesis and hypotensive response

Abstract The aim of this study was to evaluate antioxidant, antihyperlipidemic and hypotensive properties of pomegranate peel (PP) on antioxidant status, fat deposition, lipid peroxidation and pulmonary hypertensive response in broiler chickens. A total of 375 one‐day‐old male broilers (Cobb 500) were randomly assigned to five treatments included dietary PP levels of 0, 2.5, 5.0, 7.5 and 10 g/kg. Supplementation of PP at 7.5 and 10 g/kg resulted in significant upregulation of hepatic catalase (p < 0.004) and superoxide dismutase1 (SOD1; p < 0.05), which reflected in decreased concentration of circulatory malondialdehyde (MDA). Dietary inclusion of PP at 7.5 and 1.0 g/kg significantly decreased serum concentrations of triglycerides (p < 0.004) and cholesterol (p < 0.006) with concomitant decrease in abdominal fat deposition (p < 0.05). The antihyperlipidemic effect of PP was mediated through down‐regulation of peroxisome proliferator activated receptor alpha (PPARα). Hypotensive effect of PP was also observed at 7.5 and 10 g/kg as reduced heart weight and the right‐to‐total ventricular weight ratio (RV/TV) and decreased mortality from pulmonary hypertension. The hypotensive property of PP was associated with increased concentration of serum nitric oxide. In conclusion, this study revealed antioxidative, antihyperlipidemic and hypotensive effects of PP at 7.5 and 10 g/kg in broiler chickens exposed to hypobaric hypoxia. Health‐beneficial effects of PP suggest this product as a promising multi‐functional phytogenic feed additive for broiler chickens.
Rate processes of chlorine species distribution during chlorine dioxide prebleaching of kraft pulp

The distribution of atomic chlorine over all chlorine containing species such as chloride, chlorine (hypochlorous acid), chloritc, chlorine dioxide and chlorate as well as organically bound chlorine in the pulp and bleach solution, was measured during C10, prebleaching of kraft pulp. The chlorine mass balance was satisfied throughout the bleaching reaction. Based on the development of the chlorine containing species. a series of reactions are given which explains the path of atomic chlorine during CIOl bleaching. It was found that all organic chlorine. defined as the sum of AOX in the spent C102 solution and the organically bound chlorine in the chlorinated pulp, is formed within the first 10 min of reaction. Subsequently. the increase in AOX in solution is matched by an equivalent decrease of organically bound chlorine in the pulp.
Selatogrel: A Novel Subcutaneous P2Y12 Inhibitor

Abstract: The use of a P2Y12 inhibitor as a component of dual antiplatelet therapy in patients with an acute coronary syndrome (ACS) is well established. However, the P2Y12 inhibitors currently available have pharmacokinetic limitations due to delayed absorption, lack of enteral access for administration with oral formulations, need for intravenous access with cangrelor, or need for metabolization to be ideal in the critical 3-hour window during an ACS. Selatogrel is a novel, potent, reversible, and selective 2-phenylprimdine-4-carboxamide administered subcutaneously under development. Results from preclinical, phase 1, and phase 2 trials have confirmed that the agent provides sustained and reversible P2Y12 platelet inhibition with an acceptable safety profile. The most commonly reported adverse effects include minor bleeding and dyspnea. Phase 3 trials are being designed to understand the critical role this agent can play in upstream management of patients with ACS including a more defined understanding of the adverse effect profile, how to transition from this agent to an oral agent, who will be administering, and does this agent allow for a safe and quick transition to coronary artery bypass graft surgery if needed. Should it obtain approval, selatogrel has the potential to provide a unique and advantageous mechanism for P2Y12 inhibition.
Toward a Liver Cell Atlas: Understanding Liver Biology in Health and Disease at Single-Cell Resolution

Abstract Single-cell technologies are revolutionizing our understanding of cellular heterogeneity and functional diversity in health and disease. Here, we review the current knowledge and advances in liver biology using single-cell approaches. We focus on the landscape of the composition and the function of cells in a healthy liver in the context of its spatial organization. We also highlight the alterations of the molecular landscape in chronic liver disease and liver cancer, which includes the identification of disease-related cell types, altered cellular functions, dynamic cell–cell interactions, the plasticity of malignant cells, the collective behavior of a cell community, and microenvironmental reprogramming. We anticipate that the uncovered liver cell atlas will help deciphering the molecular and cellular mechanisms driving a healthy liver into a disease state. It also offers insight into the detection of new therapeutic targets and paves the way for effective disease interventions.
Forkhead box P1 (Foxp1) in osteoblasts regulates bone mass accrual and adipose tissue energy metabolism

Adiponectin (AdipoQ), a hormone abundantly secreted by adipose tissues, has multiple beneficial functions, including insulin sensitization as well as lipid and glucose metabolism. It has been reported that bone controls energy metabolism through an endocrine‐based mechanism. In this study, we observed that bone also acts as an important endocrine source for AdipoQ, and its capacity in osteoblasts is controlled by the forkhead box P1 (FOXP1) transcriptional factor. Deletion of the Foxp1 gene in osteoblasts led to augmentation of AdipoQ levels accompanied by fueled energy expenditure in adipose tissues. In contrast, overexpression of Foxp1 in bones impaired AdipoQ secretion and restrained energy consumption. Chromatin immunoprecipitation sequencing (ChIP‐seq) analysis revealed that AdipoQ expression, which increases as a function of bone age, is directly controlled by FOXP1. Our results indicate that bones, especially aged bones, provide an important source of a set of endocrine factors, including AdipoQ, that control body metabolism. © 2021 American Society for Bone and Mineral Research (ASBMR).
From Proteinuria to Fibrosis: An Update on Pathophysiology and Treatment Options

Background: Proteinuria is a key biomarker in nephrology. It is central to diagnosis and risk assessment and the primary target of many important therapies. Etiologies resulting in pathological proteinuria include congenital and acquired disorders, as well as both glomerular (immune/non-immune mediated) and tubular defects. Summary: Untreated proteinuria is strongly linked to progressive loss of kidney function and kidney failure. Excess protein reaching the renal tubules is ordinarily resorbed by the tubular epithelium. However, when these mechanisms are overwhelmed, a variety of inflammatory and fibrotic pathways are activated, causing both interstitial fibrosis and glomerulosclerosis. Nevertheless, the specific mechanisms underlying this are complex and remain incompletely understood. Recently, a number of treatments, in addition to angiotensin system blockade, have been shown to effectively slow the progression of proteinuric chronic kidney disease. However, additional therapies are clearly needed. Key message: This review provides an update on the pathophysiology of proteinuria, the pathways leading to fibrosis, and an overview of current and emerging therapies.
The Oligosaccharyltransferase AglB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis

Methanogenic archaea are responsible for producing the majority of methane on Earth and catalyze the terminal reactions in the degradation of organic matter in anoxic environments. Methanogens often grow as biofilms associated with surfaces or partner organisms; however, the molecular details of surface-associated growth remain uncharacterized. ABSTRACT Most microbial organisms grow as surface-attached communities known as biofilms. However, the mechanisms whereby methanogenic archaea grow attached to surfaces have remained understudied. Here, we show that the oligosaccharyltransferase AglB is essential for growth of Methanococcus maripaludis strain JJ on glass or metal surfaces. AglB glycosylates several cellular structures, such as pili, archaella, and the cell surface layer (S-layer). We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. A strain JJ mutant lacking structural components of the type IV-like pilus did not have a growth defect under any conditions tested, while a mutant lacking the preflagellin peptidase (ΔflaK) was defective for surface growth only when formate was provided as the sole electron donor. Finally, for strains that are capable of Fe0 oxidation, we show that deletion of aglB decreases the rate of anaerobic Fe0 oxidation, presumably due to decreased association of biomass with the Fe0 surface. Together, these data provide an initial characterization of surface-associated growth in a member of the methanogenic archaea. IMPORTANCE Methanogenic archaea are responsible for producing the majority of methane on Earth and catalyze the terminal reactions in the degradation of organic matter in anoxic environments. Methanogens often grow as biofilms associated with surfaces or partner organisms; however, the molecular details of surface-associated growth remain uncharacterized. We have found evidence that glycosylation of the cell surface layer is essential for growth of M. maripaludis on surfaces and can enhance rates of anaerobic iron corrosion. These results provide insight into the physiology of surface-associated methanogenic organisms and highlight the importance of surface association for anaerobic iron corrosion.
Heterogeneous clinical and pathological landscapes of HER2 positive colorectal cancer

ABSTRACT Introduction: Metastatic (m) colorectal cancer (CRC) can be divided into specific subgroups under the ‘one gene, one drug’ paradigm of precision medicine. Progress of targeted therapy in mCRC patients significantly improved the overall survival rate, notably by therapy targeting of EGFR signaling in RAS wild-type mCRC patients. Activation of the HER2 pathway is an important mechanism of resistance for anti-EGFR therapy. Area covered: Inhibition of HER2 with monoclonal antibodies and/or tyrosine kinase inhibitors induces tumor responses in partial HER2-positive CRC refractory to standard systemic therapy. This manuscript aimed to provide an overall insight of the HER2 expression pattern and highlighted specific clinicopathological and molecular features involved in mCRC. In addition, we summarize preclinical and clinical trials in HER2-positive mCRC. Expert opinion: The status and progression of HER2-positive gastric cancer and breast cancer and anti-HER2 therapy have been reported widely. However, the understanding of HER2-positive CRC models which may guide future therapeutic decision-making is poor. Therefore, it is essential to summarize the existing research to extract similarity and difference among various studies.