Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in adipokine concentrations in antidepressant-resistant bipolar depression after ketamine infusion and electroconvulsive therapy

Abstract Objectives This study attempts to assess the concentration of two opposite-acting adipokines (anti-inflammatory adiponectin and pro-inflammatory resistin) in antidepressant-resistant patients undergoing ketamine infusion (KI) and electroconvulsive therapy (ECT). Methods The study group comprised 52 patients hospitalised due to episodes of depression in the course of bipolar disorders. The Hamilton depression scale was used to assess the intensity of the depression symptoms before starting therapy and one day after its completion. The serum concentration of adipokines was determined before and after the therapeutic intervention using an ELISA method. Results Baseline adipokine levels differed between patients receiving KI and ECT therapy. Regardless of the procedure used, these levels changed after treatment, with the nature of these changes being different. In the case of KI, the adiponectin levels increased, and resistin levels decreased. In contrast, after ECT, the concentrations of both adipokines decreased. Changes in adipokine concentrations correlated with improvement in mental status, as assessed by the Hamilton Rating Scale, type of bipolar disorder, and gender. Conclusions Adipokines remain interesting candidate biomarkers in assessing the state and course of the disease depending on the therapeutic procedure applied. However, the relatively small study group and limited original research available for discussion justify further investigation.
Reimagining consumer involvement: Resilient system indicators in the COVID‐19 pandemic response in New South Wales, Australia

Reflections on the response to the COVID‐19 pandemic often evoke the concept of ‘resilience’ to describe the way health systems adjusted and adapted their functions to withstand the disturbance of a crisis, and in some cases, improve and transform in its wake. Drawing from this, this study focuses on the role of consumer representatives in healthcare services in initiating changes to the way they participated in the pandemic response in the state of New South Wales in Australia.
Eleutheroside E functions as anti-cervical cancer drug by inhibiting the phosphatidylinositol 3-kinase pathway and reprogramming the metabolic responses.

OBJECTIVES Cervical cancer (CC) is the common female malignant tumour with non-negligible morbidity and mortality. Eleutheroside E (EE) has anti-oxidative stress, anti-inflammatory and anti-proliferation effects in diverse disease models. However, its anti-tumour role remains unclear. METHODS The cell viability, apoptosis rate and protein expressions were detected by CCK-8, flow cytometry and western blot assays, respectively. The metabolic profile was performed by GC/MS analysis. Furthermore, the effect of EE on CC was verified in nude mice. KEY FINDINGS EE notably decreased the viability and increased the cell apoptosis, which could be reversed with 740Y-P treatment. EE treatment changed the metabolic categories of SiHa cells. The fatty acids signalling pathway was the most outstanding differential pathway. Myo-inositol prominently enhanced the level of phosphorylated Akt in a dose-dependent way. Moreover, EE declined the tumour volume and weight and the proliferation, but promoted the apoptosis in vivo. EE reduced the relative expression of phosphorylated PI3K and Akt. However, all these in-vivo results were observably antagonized with myo-inositol treatment. CONCLUSIONS EE plays an anti-tumour role in CC via inhibiting the PI3K pathway and reprogramming the metabolic responses.
Reactive Carbonyl Species Inhibit Blue Light-Dependent Activation of the Plasma Membrane H+-ATPase and Stomatal Opening.

Reactive oxygen species (ROS) play a central role in plant responses to biotic and abiotic stresses. ROS stimulate stomatal closure by inhibiting blue light-dependent stomatal opening under diverse stresses in the daytime. However, the stomatal opening inhibition mechanism by ROS remains unclear. In this study, we aimed to examine the impact of reactive carbonyl species (RCS), lipid peroxidation products generated by ROS, on blue light signaling in guard cells. Application of RCS, such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), inhibited blue light-dependent stomatal opening in the epidermis of Arabidopsis thaliana. Acrolein also inhibited H+ pumping and the plasma membrane H+-ATPase phosphorylation in response to blue light. However, acrolein did not inhibit blue light-dependent autophosphorylation of phototropins and phosphorylation of BLUE LIGHT SIGNALING1 (BLUS1). Similarly, acrolein affected neither the kinase activity of BLUS1 nor the phosphatase activity of protein phosphatase 1, a positive regulator of blue light signaling. However, acrolein inhibited fusicoccin-dependent phosphorylation of H+-ATPase and stomatal opening. Furthermore, carnosine, an RCS scavenger, partially alleviated the abscisic acid- and hydrogen peroxide-induced inhibition of blue light-dependent stomatal opening. Altogether, these findings suggest that RCS inhibit blue light signaling, especially H+-ATPase activation, and play a key role in the crosstalk between blue light and ROS signaling pathways in guard cells.
Statistical grand rounds: design and analysis of studies with binary- event composite endpoints: guidelines for anesthesia research.

Composite endpoints consisting of several binary events, such as distinct perioperative complications, are frequently chosen as the primary outcome in anesthesia studies (and in many other clinical specialties) because (1) no single outcome fully characterizes the disease or outcome of interest, and/or (2) individual outcomes are rare and statistical power would be inadequate for any single one. Interpreting a composite endpoint is challenging because components rarely meet the ideal criteria of having comparable clinical importance, frequency, and treatment effects. We suggest guidelines for forming composite endpoints and show advantages of newer versus conventional statistical methods for analyzing them. Components should be a parsimonious set of outcomes, which when taken together, well represent the disease of interest and are very plausibly related to the intervention. Adding components that are too narrow, redundant, or minimally influenced by the study intervention compromises interpretation of results and reduces power. We show that multivariate (i.e., multiple outcomes per patient) methods of analyzing a binary-event composite provide distinct advantages over standard methods such as any-versus-none, count of events, or evaluation of individual events. Multivariate methods can incorporate clinical importance weights, compensate for events occurring at varying frequencies, assess treatment effect heterogeneity, and are often more powerful than alternative statistical approaches. Methods are illustrated with an American College of Surgeons National Surgical Quality Improvement Program registry study that evaluated the effects of smoking on major perioperative outcomes, and with a clinical trial comparing the effects of crystalloids and colloids on major complications. Sample data files and SAS code are included for convenience. (Anesth Analg 2011;112:1461–71)
Prevalence of Autism Spectrum Disorder Among Children and Adolescents in the United States from 2019 to 2020.

Prevalence of Autism Spectrum Disorder Among Children and Adolescents in the United States from 2019 to 2020 Autism spectrum disorder (ASD) is a complicated neurodevelopmental disability with an increasing prevalence worldwide and considerable implications for individuals and their families.1-3 According to the Autism and Developmental Disabilities Monitoring Network, the prevalence of ASD in children aged 8 years was 2.30% in 2018, which was similar to the prevalence of 2.47% in 2014 to 2016 reported by Xu et al.2,4 The aim of the study was to estimate the ASD prevalence among children and adolescents in the US in 2019 and 2020.
‘Standing up for Myself’ (STORM): Development and qualitative evaluation of a psychosocial group intervention designed to increase the capacity of people with intellectual disabilities to manage and resist stigma

Abstract Background People with intellectual disabilities are at risk of experiencing stigma and require the skills and confidence to deal with stigma in their daily lives. Method Development and piloting of a 5‐session manualised psychosocial group intervention designed to increase the capacity of people with intellectual disabilities aged 16+ to manage and resist stigma. Ten pre‐existing groups (N = 67) in third sector and education settings participated. Interviews with participants (n = 26), facilitators (n = 9) and significant others (n = 7) 2–4 months after the intervention assessed perceived impact. Results Perceived benefits of the intervention for participants included increased understanding, improved connections with others, drive for advocacy, increased activity and self‐efficacy, and opportunity to process difficult events and emotions. Differential impact depending on individuals' pre‐existing self‐advocacy skills was noted. Conclusions This early‐stage study indicates that further evaluation is merited to examine feasibility and outcomes of the STORM intervention.
PARD3 gene variation as candidate cause of nonsyndromic cleft palate only

Nonsyndromic cleft palate only (NSCP) is a common congenital malformation worldwide. In this study, we report a three‐generation pedigree with NSCP following the autosomal‐dominant pattern. Whole‐exome sequencing and Sanger sequencing revealed that only the frameshift variant c.1012dupG [p. E338Gfs*26] in PARD3 cosegregated with the disease. In zebrafish embryos, ethmoid plate patterning defects were observed with PARD3 ortholog disruption or expression of patient‐derived N‐terminal truncating PARD3 (c.1012dupG), which implicated PARD3 in ethmoid plate morphogenesis. PARD3 plays vital roles in determining cellular polarity. Compared with the apical distribution of wild‐type PARD3, PARD3‐p. E338Gfs*26 mainly localized to the basal membrane in 3D‐cultured MCF‐10A epithelial cells. The interaction between PARD3‐p. E338Gfs*26 and endogenous PARD3 was identified by LC–MS/MS and validated by co‐IP. Immunofluorescence analysis showed that PARD3‐p. E338Gfs*26 substantially altered the localization of endogenous PARD3 to the basement membrane in 3D‐cultured MCF‐10A cells. Furthermore, seven variants, including one nonsense variant and six missense variants, were identified in the coding region of PARD3 in sporadic cases with NSCP. Subsequent analysis showed that PARD3‐p. R133*, like the insertion variant of c.1012dupG, also changed the localization of endogenous full‐length PARD3 and that its expression induced abnormal ethmoid plate morphogenesis in zebrafish. Based on these data, we reveal PARD3 gene variation as a novel candidate cause of nonsyndromic cleft palate only.
Identi ﬁ cation and characterization of sex-dependent gene expression pro ﬁ le in glioblastoma

Glioblastoma (GBM) is the most lethal primary tumor in the human brain and lacks favorable treatment options. Sex differences in the outcome of GBM are broadly acknowledged, but the underlying molecular mechanisms remain largely unknown. To identify the sex-dependent critical genes in the progression of GBM, raw data from several microarray datasets with the same array platform were downloaded from the Gene Expression Omnibus (GEO) database. These datasets included tumorous and normal tissue from patients with GBM and crucial sex features. Then, the differentially expressed genes (DEGs) in female and male tumors were identi ﬁ ed via bioinformatics analysis, respectively. Functional signatures of the identi-ﬁ ed DEGs were further annotated by Gene Ontology (GO) and pathway enrichment analyses. Venn diagram and functional protein – protein interaction (PPI) network analyses were performed to screen out the sex-speci ﬁ c DEGs. Survival analysis of patients with differences in the expression level of selected genes was then carried out using the data from The Cancer Genome Atlas (TCGA). Here, we showed that ECT2, AURKA, TYMS, CDK1, NCAPH, CENPU, OIP5, KIF14, ASPM, FBXO5, SGOL2, CASC5, SHCBP1, FN1, LOX, IGFBP3, CSPG4, and CD44 were enriched in female tumor samples, whereas TNFSF13B, CXCL10, CXCL8, CXCR4, TLR2, CCL2, and FCGR2A were enriched in male tumor samples. Among these key genes, interestingly, ECT2 was associated with increased an survival rate for female patients, whileTNFSF13B could be regarded as a potential marker of poor prognosis in male patients. These results suggested that sex differences in patients may be attrib-uted to the heterogeneous gene activity, which might in ﬂ u-ence the oncogenesis and the outcomes of GBM
Mental Representations of Sickness Positively Relate to Adaptive Health Behaviors

An ecological approach to social perception states that impressions of faces have functional value in that they guide adaptive behavior ensuring people's survival. For example, people may avoid others whose faces appear sick to avoid an illness representing a survival threat. We broadened the ecological approach in the current work by examining whether merely thinking about what illnesses on faces look like (i.e., how sickness on faces is represented) holds functional value in guiding behavior to ensure survival. Using an example of a real illness threat as proof of concept, we showed that people self-reported performing more adaptive health behaviors in response to COVID-19 if they had sicker representations of COVID-19 on faces (Experiment 1a). These sicker representations of COVID-19 on faces explained, in part, a positive relation between perceptions of COVID-19 as threatening and people's self-reported adaptive health behaviors. We then replicated these patterns when experimentally manipulating illness threat (Experiment 1b). We found that people expected more adaptive health behaviors and had sicker representations of illness on faces in response to illness threats that were more relative to less threatening. These findings suggest that mentally representing sickness on faces is enough to guide people's behaviors in response to illness threats.
Precision medicine in chronic obstructive pulmonary disease

Abstract Over the last 20 years, it has become possible to use a precision medicine approach to the management of chronic obstructive pulmonary disease (COPD). Clinical and physiological features as well as a blood biomarker can be used to target treatments to patients most likely to benefit and avoid treatment in patients less likely to benefit. Future advances in a precision medicine approach to COPD will depend on more precise characterization of individual patients, possibly using quantitative imaging, new physiological techniques, novel biomarkers and genetic profiling. Precision medicine has led to significant improvements in the management of COPD and clinicians should use all available information to optimize the treatment of individual patients.
Knockout of cardiac troponin I‐interacting kinase leads to cardiac dysfunction and remodelling

Cardiac troponin I‐interacting kinase (TNNI3K) is a cardiac‐specific kinase that has been identified as a diagnostic marker and a therapeutic target in cardiovascular diseases. However, the biological function of TNNI3K in cardiac dysfunction and remodelling remains elusive. In the present study, a Tnni3k cardiomyocyte‐specific knockout (Tnni3k‐cKO) mouse model was established. Echocardiography was used to evaluate cardiac function in mice. Heart failure markers were detected using enzyme‐linked immunosorbent assay. Haematoxylin and eosin staining, wheat germ agglutinin staining, Masson's trichrome staining, Sirius red staining and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining were used to assess histopathological changes, cardiac hypertrophy, collagen deposition and myocardial apoptosis, respectively. Expression levels of TNNI3K, apoptosis‐related proteins, and p38 mitogen‐activated protein kinase were measured using Western blot analysis. Compared to wild‐type controls, cardiac dysfunction and cardiac remodelling of Tnni3k‐cKO mice increased gradually with age. Tnni3k‐cKO mice exhibited cardiac hypertrophy, cardiac fibrosis and cardiomyocyte apoptosis. Upregulation of cleaved caspase‐3 in Tnni3k‐cKO mice appeared to be related to phosphorylation and activation of the p38 mitogen‐activated protein kinase signalling pathway. In conclusion, this study shows that TNNI3K is essential for cardiac development and function, providing new insights into the development of novel therapeutic strategies for cardiac diseases.
Avoiding Covid‐19 risk information in the United States: The role of attitudes, norms, affect, social dominance orientations, and perceived trustworthiness of scientists

This study seeks guidance from the planned risk information avoidance model to explore drivers of risk information avoidance in the context of COVID‐19. Data were collected early during the pandemic. Among our most notable results is that participants who are more oriented toward social dominance and are more skeptical of scientists’ credibility have (1) more supportive attitudes toward risk information avoidance and (2) feel social pressure to avoid risk information. The findings of this study highlight how the role of skepticism in science and intergroup ideologies, such as social dominance, can have important implications for how people learn about health‐related information, even in times of heightened crisis.
Sistematização da assistência de enfermagem em Centro Obstétrico

Trata-se de um estudo descritivo-exploratorio, com abordagem quantitativa, que objetivou propor um protocolo para a Sistematizacao da Assistencia de Enfermagem para parturientes no Centro Obstetrico de um hospital publico em Recife-PE. Foi aplicado um instrumento semiestruturado a quarenta parturientes para a obtencao do historico de enfermagem; a partir deste foram identificados os diagnosticos de enfermagem baseados na Classificacao Internacional para a Pratica de Enfermagem (CIPE®), versao 1, e estabelecidos os respectivos resultados e intervencoes de enfermagem. O protocolo consiste em duas etapas: a primeira e a consulta de enfermagem, que envolve a anamnese e exame fisico, e a segunda e caracterizada pela identificacao criteriosa dos diagnosticos de enfermagem, que direcionarao o planejamento da assistencia de enfermagem para o atendimento individualizado as parturientes, com utilizacao de uma terminologia universal.
Dormancy-release, germination and seedling growth of Paeonia ostii ‘Fengdan’ seeds under measures of physical and chemical treatment and sowing

Paeonia ostii ‘Fengdan’, a woody oleaginous plant native from China, is considered an oil crop with economic potential. However, a low germination rate was still a restriction for Paeonia ostii ‘Fengdan’ production. The present research evaluated the germination, rooting and physiological characteristics of seedlings of Paeonia ostii ‘Fengdan’ in response to different physical treatments and the application of exogenous chemicals. Results indicated that seeds stored in sand at room temperature, and soaked in water for 3 days prior to planting, had a beneficial effect on hypocotyl dormancy-breaking. The rate of rooting and root growth of Paeonia ostii ‘Fengdan’ were significantly improved with 5 cm sowing depth in 15–20℃ soils. Compared with other sowing depths, the rooting percentage was significantly increased by 1.19% (2.5 cm), 0.98% (7.5 cm) and 1.47% (10 cm), respectively. Epicotyl dormancy was relieved when taproot length reached 50 mm. Soaking seeds in 0.76 mmol/L 5-aminolevulinic acid for 48 hours had the greatest beneficial effect on seed germination and seedling growth, the germination percentage was significantly increased by 4.25% (24 h) and 5.08% (72 h) compared with other treatments. While seed soaked in 10 mmol/L sodium nitroprusside for 48 hours also exhibited enhanced seedling growth, and the germination percentage was significantly increased by 4.36% (24 h) and 7.40% (72 h). Those results benefited seed germination and seedling growth of Paeonia ostii ‘Fengdan’ which could suggest the promotion of its industrial values and productive potentials. The mechanism of seed breaking dormancy and germination of Paeonia ostii ‘Fengdan’ needs further study.
Identifying conserved genes involved in crop tolerance to cold stress.

Low temperature is a limiting factor for crop productivity in tropical and subtropical climates. Cold stress response in plants involves perceiving and relaying the signal through a transcriptional cascade composed of different transduction components, resulting in altered gene activity. We performed a meta-analysis of four previously published datasets of cold-tolerant and cold-sensitive crops to better understand the gene regulatory networks and identify key genes involved in cold stress tolerance conserved across phylogenetically distant species. Re-analysing the raw data with the same bioinformatics pipeline, we identified common cold tolerance-related genes. We found 236 and 242 commonly regulated genes in sensitive and tolerant genotypes, respectively. Gene enrichment analysis showed that protein modifications, hormone metabolism, cell wall, and secondary metabolism are the most conserved pathways involved in cold tolerance. Upregulation of the abiotic stress (heat and drought/salt) related genes [heat shock N-terminal domain-containing protein, 15.7kDa class I-related small heat shock protein-like, DNAJ heat shock N-terminal domain-containing protein, and HYP1 (HYPOTHETICAL PROTEIN 1)] in sensitive genotypes and downregulation of the abiotic stress (heat and drought/salt) related genes (zinc ion binding and pollen Ole e 1 allergen and extensin family protein) in tolerant genotypes was observed across the species. Almost all development-related genes were upregulated in tolerant and downregulated in sensitive genotypes. Moreover, protein-protein network analysis identified highly interacting proteins linked to cold tolerance. Mapping of abiotic stress-related genes on analysed species genomes provided information that could be essential to developing molecular markers for breeding and building up genetic improvement strategies using CRISPR/Cas9 technologies.