Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessing small-mammal trapping design using spatially explicit capture recapture (SECR) modeling on long-term monitoring data

Few studies have evaluated the optimal sampling design for tracking small mammal population trends, especially for rare or difficult to detect species. Spatially explicit capture-recapture (SECR) models present an advancement over non-spatial models by accounting for individual movement when estimating density. The salt marsh harvest mouse (SMHM; Reithrodontomys raviventris) is a federal and California state listed endangered species endemic to the San Francisco Bay-Delta estuary, California, USA; where a population in a subembayment has been continually monitored over an 18-year period using mark-recapture methods. We analyzed capture data within a SECR modeling framework that allowed us to account for differences in detection and movement between sexes. We compared the full dataset to subsampling scenarios to evaluate how the grid size (area) of the trap design, trap density (spacing), and number of consecutive trapping occasions (duration) influenced density estimates. To validate the subsampling methods, we ran Monte Carlo simulations based on the true parameter estimates for each specific year. We found that reducing the area of the trapping design by more than 36% resulted in the inability of the SECR model to replicate density estimates within the SE of the original density estimates. However, when trapping occasions were reduced from 4 to 3-nights the density estimates were indistinguishable from the full dataset. Furthermore, reducing trap density by 50% also resulted in density estimates comparable to the full dataset and was a substantially better model than reducing the trap area by 50%. Overall, our results indicated that moderate reductions in the number of trapping occasions or trap density could yield similar density estimates when using a SECR approach. This approach allows the optimization of field trapping efforts and designs by reducing field efforts while maintaining the same population estimate compared to the full dataset. Using a SECR approach may help other wildlife programs identify sampling efficiencies without sacrificing data integrity for long term monitoring of population densities.
Environmental light exposure and mealtime regularity: Implications for human health

ABSTRACT Light exposure and mealtime act as cues to the human circadian rhythm, which subsequently regulates various physiological functions in the body. However, modernization alters lifestyles, with changes to social and work-related activities independent of the natural light–dark cycle. This review summarizes the role of light exposure and regular mealtime on bodily processes and, ultimately, metabolic health. Various aspects of light are reviewed, including the type of light (natural/artificial), intensity (lux), spectral composition, time of exposure (night/day), and exposure duration. Further, the possible relationship between light exposure and mealtime irregularity is discussed as a function affecting metabolic health. In essence, research evidence suggests that mealtime regularity and light exposure habits based on the natural occurring light–dark cycle are essential for metabolic health in relation to an aligned circadian rhythm.
Thermal trait variation may buffer Southern Ocean phytoplankton from anthropogenic warming

Despite the potential of standing genetic variation to rescue communities and shape future adaptation to climate change, high levels of uncertainty are associated with intraspecific trait variation in marine phytoplankton. Recent model intercomparisons have pointed to an urgent need to reduce uncertainty in the projected responses of marine ecosystems to climate change, including Southern Ocean (SO) surface waters, which are among the most rapidly warming habitats on Earth. Because SO phytoplankton growth responses to warming sea surface temperature (SST) are poorly constrained, we developed a high‐throughput growth assay to simultaneously examine inter‐ and intra‐specific thermal trait variation in a group of 43 taxonomically diverse and biogeochemically important SO phytoplankton called diatoms. We found significant differential growth performance among species across thermal traits, including optimum and maximum tolerated growth temperatures. Within species, coefficients of variation ranged from 3% to 48% among strains for those same key thermal traits. Using SO SST projections for 2100, we predicted biogeographic ranges that differed by up to 97% between the least and most tolerant strains for each species, illustrating the role that strain‐specific differences in temperature response can play in shaping predictions of future phytoplankton biogeography. Our findings revealed the presence and scale of thermal trait variation in SO phytoplankton and suggest these communities may already harbour the thermal trait diversity required to withstand projected 21st‐century SST change in the SO even under severe climate forcing scenarios.
Methods recently used for the assessment of physical activity in children and adolescents

Purpose of review The aim of this review is to summarize recent evidences and advances on the implementation and the use of new tools to assessing physical activity (PA) in children. Recent findings Technological advances provide, increasingly, new objective methods for the evaluation of PA in children. In addition to accelerometry, there are other objective methods for assessing PA in children such as new wearable monitoring and activity bracelets, smartphone and recording software applications, Global Positioning System or Inertial Sensors Devices. Summary Doubly labeled water and calorimetry are reference methods to assessing PA but with limitations of use. Accelerometry is an accurate method for measuring sedentary behavior and PA levels in children. In fact, it is a real alternative reference method for the validation of methods and tools of assessing PA. However, there is still no consensus about the most appropriate approach to analyze the duration and intensity of PA in children. Therefore, the implementation of other alternative objective methods, as well as complementation with PA questionnaires, can provide a more precise evaluation of different patterns and behaviors related with sedentarism and PA.
Norepinephrine versus phenylephrine for post-spinal hypotension in parturients undergoing caesarean section: a systematic review and meta-analysis.

INTRODUCTION Multiple studies have compared varying prophylactic and therapeutic doses of norepinephrine and phenylephrine given as either intermittent bolus or fixed-rate infusion to combat post-spinal hypotension in patients undergoing cesarean section (CS). We conducted a systematic review to figure out the best alternative to treat post-spinal hypotension. EVIDENCE ACQUISITION PubMed and Cochrane databases were extensively searched for eligible RCTs. A total of 15 studies were found eligible and analysed for the incidence of maternal bradycardia as the primary outcome and other maternal adverse effects, fetal acidosis and Apgar scores at 1 and 5 min as the secondary outcome. Data was analysed using Review Manager Version 5.3. software. EVIDENCE SYNTHESIS There was no significant difference in the efficacy of norepinephrine and phenylephrine for managing post-spinal hypotension [OR = 1.15 (95% CI=0.91-1.45),P=0.24, I2=0%,moderate quality] in parturients undergoing CS. Odds of incidence of maternal bradycardia decrease significantly by 61% with norepinephrine versus phenylephrine [OR = 0.39 (95% CI = 0.31-0.49),P<0.00001, I2=27%,high quality evidence]. Significant higher umbilical artery mean pH values were observed with NE versus PE [MD = 0.0 (95%CI=0.00 to 0.01),P=0.03], although not clinical relevant. However, no significant difference was found in the incidence of other maternal adverse effects and fetal outcomes. CONCLUSIONS Comparable efficacy for management of post-spinal hypotension, though, norepinephrine was found to cause less incidence of maternal bradycardia as compared to phenylephrine.
TRANSMEMBRANE EXTRACELLULAR MATRIX – CYTOSKELETON CROSSTALK

Cell interactions with the extracellular matrix (ECM) and with neighbouring cells trigger numerous responses that have essential roles in the regulation of their behaviour and fate. ECM adhesion and assembly affect cells in many ways. As the ECM provides the physical microenvironment in which cells live, it provides a substrate for cell anchorage and serves as a tissue scaffold, guides cell migration during embryonic development and wound repair, and has other key roles in tissue morphogenesis. However, beyond these obvious scaffolding functions, the ECM is also responsible for transmitting environmental signals to cells, which affect essentially all aspects of a cell’s life, including its proliferation, differentiation and death. So, selection of the appropriate matrix for experiments using cultured cells is crucial, as it can profoundly affect the cellular response. As will be discussed below, not only is the molecular composition of the ECM important, but also its topography and mechanical properties. These features of the ECM are determined both by the cells that produce it and by the cells that grow on or inside the matrix. So, the life of a cell seems to involve intense and complex crosstalk with the matrix. In this review, we focus on the molecular events that occur at both faces of the cell membrane in cell–matrix adhesions. We describe briefly the molecular organization and complexity of the membrane–cytoskeleton interface in this region, as well as external interactions with the ECM. We show how cells actively shape the matrix around them, and that the entire process is controlled both locally and globally by specific regulatory pathways, with a mechanosensory system and molecular switches acting as pivotal elements. We also consider several puzzles in rapidly developing areas that provide intriguing opportunities for new advances.
Pre-Existing Right Ventricular Dysfunction as an Independent Risk Factor for Post Intubation Cardiac Arrest and Hemodynamic Instability in Critically Ill Patients: A Retrospective Observational Study

Background Post intubation cardiac arrest and hemodynamic instability are serious adverse events encountered in critically ill patients. The association of pre-existing right ventricular (RV) dysfunction with post intubation cardiac arrest and hemodynamic instability in critically ill patients is unknown. Methods This is a retrospective matched cohort study of adult critically ill patients who underwent intubation from July 2016 to December 2019. The study was conducted at a quaternary medical center in Houston, Texas. A total of 340 critically ill patients who underwent intubation in the intensive care units, wards, and the emergency room were included. The study cohort was categorized into 4 groups based on the pre-existing RV function: normal function, mild dysfunction, moderate dysfunction, and severe dysfunction. Cardiac arrest and/or hemodynamic instability within one hour post intubation were the primary study outcomes. Secondary outcomes included in hospital and 60-day mortality. Results Study patients were of mean age of 61.95 ± 14.28 years, including 132 (39%) females and 208 (61%) males. The primary outcomes were significantly worse in mild, moderate, and severe RV dysfunction groups compared to the normal RV function group (34.12%-P = 0.014, 47.06%-P < 0.001, 51.67%-P < 0.001, vs. 17.56%). In a multivariable logistic regression analysis, pre-existing moderate (OR = 2.65, P = 0.013) and severe RV dysfunction groups (OR = 2.66, P = 0.015) were associated with statistically significant higher cardiac arrest and hemodynamic instability post intubation. Pre-existing severe RV dysfunction was associated with statistically significant higher in hospital mortality (62.35%-P < 0.001). The multivariable Cox-regression analysis showed that pre-existing severe RV dysfunction was associated with a statistically significant higher 60-day mortality (HR = 2.57, P = 0.001). Conclusions Pre-existing moderate and severe RV dysfunctions were independently associated with significantly higher cardiac arrest and/or hemodynamic instability post intubation in critically ill patients. Pre-existing RV function may serve as a mortality predictor in critically ill patients undergoing endotracheal intubation.
Aberrant Whole-Brain Resting-State Functional Connectivity Architecture in Obsessive-Compulsive Disorder: An EEG Study

Obsessive-compulsive disorder (OCD) is a common neuropsychiatric disorder characterized by intrusive thoughts (obsessions) and repetitive behaviors (compulsions), and few studies have assessed the whole-brain functional connectivity architecture of OCD with electroencephalogram (EEG) during different resting states. Graph theory and network-based statistics (NBS) were employed to examine the neural synchronization and the whole-brain functional connectivity (FC) based on the phase-locking value (PLV) of OCD patients and healthy controls (HCs) during eyes-closed (EC) and eyes-open (EO) states. Compared with HCs, OCD patients exhibited not only decreased global synchronization in terms of phase synchrony but also aberrant global topological properties (decreased average shortest path lengths and normalized shortest path lengths together with increased global efficiencies and normalized clustering coefficients) together with inhibited intra-hemispheric and interhemispheric FCs during rest, which suggested an imbalance between functional integration and segregation of brain networks for OCD patients. Meanwhile, OCD patients had increased global efficiencies and normalized clustering coefficients, but decreased average clustering coefficients and normalized shortest path lengths together with significantly decreased FCs in the alpha band from EC to EO states, which suggested a dynamic switch between highly integrated (EC state) and highly specialized (EO state) modes of information processing. Moreover, the decreased FCs of OCD patients showed obvious hemispheric asymmetry within or between groups during EC and EO states, which might serve as a potential biomarker to classify OCD patients from HCs.
Therapy with mesenchymal stem cell transplantation in multiple sclerosis is ready for prime time: YES

Despite the development of highly efficient and more targeted immunotherapies for multiple sclerosis (MS), two major unmet needs still exist: (1) the need for treatment to suppress compartmentalized and meningeal inflammation in the central nervous system (CNS), which seems to drive the progression of disability1 and is less responsive to the majority of immunomodulatory drugs, and (2) the need for a treatment that may substantially promote regeneration-remyelination.
S‐Nitroso‐l‐cysteine and ventilatory drive: A pediatric perspective

Though endogenous S‐nitroso‐l‐cysteine (l‐CSNO) signaling at the level of the carotid body increases minute ventilation (v̇E), neither the background data nor the potential clinical relevance are well‐understood by pulmonologists in general, or by pediatric pulmonologists in particular. Here, we first review how regulation of the synthesis, activation, transmembrane transport, target interaction, and degradation of l‐CSNO can affect the ventilatory drive. In particular, we review l‐CSNO formation by hemoglobin R to T conformational change and by nitric oxide (NO) synthases (NOS), and the downstream effects on v̇E through interaction with voltage‐gated K+ (Kv) channel proteins and other targets in the peripheral and central nervous systems. We will review how these effects are independent of—and, in fact may be opposite to—those of NO. Next, we will review evidence that specific elements of this pathway may underlie disorders of respiratory control in childhood. Finally, we will review the potential clinical implications of this pathway in the development of respiratory stimulants, with a particular focus on potential pediatric applications.
Head hemodynamics and systemic responses during auditory stimulation

The present study aims to analyze the systemic response to auditory stimulation by means of hemodynamic (cephalic and peripheral) and autonomic responses in a broad range of auditory intensities (70.9, 77.9, 84.5, 89.5, 94.5 dBA). This approach could help to understand the possible influence of the autonomic nervous system on the cephalic blood flow. Twenty‐five subjects were exposed to auditory stimulation while electrodermal activity (EDA), photoplethysmography (PPG), electrocardiogram, and functional near‐infrared spectroscopy signals were recorded. Seven trials with 20 individual tones, each for the five intensities, were presented. The results showed a differentiated response to the higher intensity (94.5 dBA) with a decrease in some peripheral signals such as the heart rate (HR), the pulse signal, the pulse transit time (PTT), an increase of the LFnu power in PPG, and at the head level a decrease in oxygenated and total hemoglobin concentration. After the regression of the visual channel activity from the auditory channels, a decrease in deoxyhemoglobin in the auditory cortex was obtained, indicating a likely active response at the highest intensity. Nevertheless, other measures, such as EDA (Phasic and Tonic), and heart rate variability (Frequency and time domain) showed no significant differences between intensities. Altogether, these results suggest a systemic and complex response to high‐intensity auditory stimuli. The results obtained in the decrease of the PTT and the increase in LFnu power of PPG suggest a possible vasoconstriction reflex by a sympathetic control of vascular tone, which could be related to the decrease in blood oxygenation at the head level.
Latent profile analysis of passion for work and its relationship with psychological well-being.

BACKGROUND So far, studies based on the dualistic model of passion have ignored how the 2 different types of passion interact in a person's identity. The aim of this article is to identify profiles of passion for work and their consequences for psychological well-being. MATERIAL AND METHODS The survey was conducted on a sample of 522 employees of various employment sectors. The Passion Scale was used to assess passion for work, while to explore well-being, the anxiety and depression subscale of the General Health Questionnaire (GHQ-28) and the Subjective Vitality Scale were employed. Latent profile analysis (LPA) was used to distinguish the passion profiles, following which the results regarding well-being in the following groups were compared using non-parametric tests. RESULTS Four passion profiles have been identified. They are termed as high-moderate (profile 1: high harmonious passion for work and moderate obsessive passion for work), high (profile 2: high harmonious passion for work and obsessive passion for work), optimal (profile 3: high harmonious passion for work and low obsessive passion for work), and low (profile 4: low harmonious passion for work and low obsessive passion for work). Employees with a low score for both harmonious passion for work and obsessive passion for work had the lowest score regarding well-being. CONCLUSIONS This study was one of the first to use the LPA approach to explore the configuration of passion for work. It provided an indication of how the different dimensions and levels of passion set up with each other and what their consequences would be. The conducted research emphasized the protective role of the harmonious passion for work against the negative effect of obsessive passion for work. Med Pr. 2022;73(4).
Development of a Tethered mRNA Amplifier to increase protein expression

Herein, we present a novel method to specifically increase a messenger RNA's (mRNA) expression at the post‐transcriptional level. This is accomplished using what we term a “Tethered mRNA Amplifier. ” The Tethered mRNA Amplifier specifically binds an mRNA's 3′ untranslated region and enhances its stability/translation, often doubling protein output. We test this approach on several transcripts associated with haploinsufficiency disorders and increase their steady‐state expression in cell culture. We suggest this approach may be a tenable therapeutic modality with precise activity and broad‐spectrum application.
Unfocused Field Analysis of a Density-Tapered Spiral Array for High-Volume-Rate 3-D Ultrasound Imaging

Spiral array transducers with a sparse 2-D aperture have demonstrated their potential in realizing 3-D ultrasound imaging with reduced data rates. Nevertheless, their feasibility in high-volume-rate imaging based on unfocused transmissions has yet to be established. From a metrology standpoint, it is essential to characterize the acoustic field of unfocused transmissions from spiral arrays not only to assess their safety but also to identify the root cause of imaging irregularities due to the array’s sparse aperture. Here, we present a field profile analysis of unfocused transmissions from a density-tapered spiral array transducer (256 hexagonal elements, 220-<inline-formula> <tex-math notation="LaTeX">$\mu \text{m}$ </tex-math></inline-formula> element diameter, and 1-cm aperture diameter) through both simulations and hydrophone measurements. We investigated plane- and diverging-wave transmissions (five-cycle, 7.5-MHz pulses) from 0° to 10° steering for their beam intensity characteristics and wavefront arrival time profiles. Unfocused firings were also tested for B-mode imaging performance (ten compounded angles, −5° to 5° span). The array was found to produce unfocused transmissions with a peak negative pressure of 93.9 kPa at 2 cm depth. All transmissions steered up to 5° were free of secondary lobes within 12 dB of the main beam peak intensity. All wavefront arrival time profiles were found to closely match the expected profiles with maximum root-mean-squared errors of <inline-formula> <tex-math notation="LaTeX">$0.054~\mu \text{s}$ </tex-math></inline-formula> for plane wave (PW) and <inline-formula> <tex-math notation="LaTeX">$0.124~\mu \text{s}$ </tex-math></inline-formula> for diverging wave. The B-mode images showed good spatial resolution with a penetration depth of 22 mm in PW imaging. Overall, these results demonstrate that the density-tapered spiral array can facilitate unfocused transmissions below regulatory limits (mechanical index: 0.034; spatial-peak, pulse-average intensity: 0.298 W/cm2) and with suppressed secondary lobes while maintaining smooth wavefronts.