Miracle-dz/UniFAR-FTUs huggingface dataset:
A clinical practice guideline for the screening and assessment of enthesitis in patients with spondyloarthritis

Objective The aim of this review is to provide guidance on the selection of approaches to the screening and assessment of enthesitis in patients with spondyloarthritis (SpA). Methods Twenty-four questions regarding the approaches to the screening and assessment of enthesitis and the implementation details were devised, followed by a systemic literature review. The Grading of Recommendations Assessment, Development, and Evaluation methodology was employed in the development of this guideline, with modifications to evaluate non-interventional approaches under comprehensive consideration of costs, accessibility, and evidence strength. A consensus from the voting panel was required for the inclusion of the final recommendations and the strength of each recommendation. Results Seventeen recommendations (including five strong recommendations) were included in this guideline. The voting panel expressed unequivocal support for the necessity of screening and assessment of enthesitis in patients with SpA. It was agreed unanimously that symptom evaluation and physical examination should serve as the initial steps to the recognition of enthesitis, whereas Maastricht Ankylosing Spondylitis Enthesitis Score is a reliable tool in both clinical trials and daily medical practice. Ultrasound examination is another reliable tool, with power Doppler ultrasound as an informative addition. Notwithstanding its high resolution, MRI is limited by the costs and relatively low accessibility, whereas radiographs had low sensitivity and therefore should be rendered obsolete in the assessment of enthesitis. PET/CT was strongly opposed in the detection of enthesitis. Conclusion This guideline provides clinicians with information regarding the screening and assessment of enthesitis in patients with SpA. However, this guideline does not intend on dictating choices, and the ultimate decisions should be made in light of the actual circumstances of the facilities.
Stems matter: Xylem physiological limits are an accessible and critical improvement to models of plant gas exchange in deep time

The evolution of woody stems approximately 400 mya (middle Paleozoic) facilitated the expansion of plants and has likely affected carbon and water budgets across much of the terrestrial surface since that time. Stems are a carbon cost/sink and limit water transport from soil to leaves as it must pass through specialized xylem tissue. While leaf fossils have provided a wealth of quantitative data, including estimates of plant water fluxes utilizing biophysically based models, fossil-informed models integrating stem and leaf physiology are lacking. Integrated stem-leaf physiology may distinguish successors to ecological catastrophes like the end of the Late Paleozoic Ice Age (LPIA). The documented collapse of LPIA tropical forests provides an opportunity to assess the importance of woody stems as a key to understanding differences in survivorship among common plant taxa from the Carboniferous to the Permian. Here, we present an analysis of the limits to leaf water supply and plant function for Paleozoic forest plant types due to (1) cavitation-induced embolism and xylem blockage and (2) insufficient sapwood water transport capacity. —collectively defined here as sapwood dysfunction. We first present a modified ecosystem process model (Paleo-BGC+) that includes sapwood dysfunction. Paleo-BGC + is parameterized using measurements obtainable from fossil xylem and therefore applicable to both modern and ancient ecosystems. We then assess the effect of sapwood dysfunction on ecosystem processes based on previously published fossil leaf measurements and a new fossil xylem dataset for plant types present in the Late Paleozoic. Using daily meteorology from a GCM of the late Carboniferous (GENESIS v3) under a Glacial (low-CO2) and an Inter-glacial (high-CO2) scenario, we found that simulated sapwood dysfunction slowed plant water use and reduced carbon storage. This inhibition occurred particularly in plants with high maximum stomatal conductance and high stem vulnerability to embolism. Coincidentally, plants with these traits were predominantly reduced or missing from the fossil record from the Carboniferous to the Permian. Integrating stem and leaf physiology may improve the fidelity of model representations of soil-to-atmosphere water transport through plants, simulations of long-term climate phenomena like the LPIA, and ecosystem projections under future climate change.
Rumen-protected zinc–methionine dietary inclusion alters dairy cow performances, and oxidative and inflammatory status under long-term environmental heat stress

Dairy cows are susceptible to heat stress due to the levels of milk production and feed intake. Dietary supplemental amino acids, particularly rate-limiting amino acids, for example, methionine (Met), may alleviate the potential negative consequences. Zinc (Zn) is beneficial to the immune system and mammary gland development during heat stress. We investigated the impact of a source of a rumen-protected Zn-Met complex (Loprotin, Kaesler Nutrition GmbH, Cuxhaven, Germany) in high-producing Holstein cows during a long-term environmental heat stress period. A total of 62 multiparous lactating Holstein cows were allocated in a completely randomized design to two dietary treatments, namely, basal diet without (control) and basal diet with the supplemental Zn-Met complex (RPZM) at 0.131% of diet DM. Cows in the RPZM group had higher energy-corrected milk (46.71 vs. 52.85 ± 1.72 kg/d for control and RPZM groups, respectively) as well as milk fat and protein concentration (27.28 vs. 32.80 ± 1.82 and 30.13 vs. 31.03 ± 0.25 g/kg for control and RPZM groups, respectively). The Zn-Met complex supplemented cows had lower haptoglobin and IL-1B concentration than the control (267 vs. 240 ± 10.53 mcg/mL and 76.8 vs. 60.0 ± 3.4 ng/L for control and RPZM groups, respectively). RPZM supplementation resulted in better oxidative status, indicated by higher total antioxidant status and lower malondialdehyde concentrations (0.62 vs. 0.68 ± 0.02 mmol/L and 2.01 vs. 1.76 ± 0.15 nmol/L for control and RPZM groups, respectively). Overall, the results from this study showed that RPZM dietary inclusion could maintain milk production and milk composition of animals during periods of heat stress. Enhanced performance of animals upon Zn-Met complex supplementation could be partly due to improved oxidative and immune status.
Late domain dependent E-cadherin recruitment into extracellular vesicles

E-cadherin, a transmembrane protein involved in epithelial cell-cell adhesion and signaling, is found in exosomal fractions isolated from human body fluids. A cellular mechanism for recruitment of E-cadherin into extracellular vesicles (EVs) has not yet been defined. Here, we show that E-cadherin is incorporated into the membrane of EVs with the extracellular domain exposed at the vesicle surface. This recruitment depends on the endosomal sorting complex required for transport I (ESCRT-I) component Tsg101 and a highly conserved tetrapeptide P(S/T)AP late domain motif in the cytoplasmic tail of E-cadherin that mediates interaction with Tsg101. Mutation of this motif results in a loss of interaction and a dramatic decrease in exosomal E-cadherin secretion. We conclude, that the process of late domain mediated exosomal recruitment is exerted by this endogenous non-ESCRT transmembrane protein.
Carbon neutrality vs. neutralité carbone: A comparative study on French and English users’ perceptions and social capital on Twitter

Carbon neutrality is one of the most critical global concerns at present. As one of the largest social media, Twitter is used widely by individuals, organisations, and government agencies to share their comments and perceptions on carbon neutrality. This study collected 26425 English and 20331 French tweets to compare the differences between French and English tweets. Social network analysis found that users in the French social networks interacted more frequently than the English ones. The geodesic analysis evidenced that the connection of any two users required about five intermediate users on average in French networks, while English ones required seven intermediate users. The modularity metrics of the English network were higher, indicating that users in English networks did not communicate with different clusters and people in carbon neutrality issues. In addition, the French network of carbon neutrality activists comprised politicians, government agencies, journalists, NGOs, and companies, while those in the English network mainly included companies, media, and politicians. Sentiment analysis and independent samples t-test have confirmed that despite the types of activists and the interactions between clusters being different, negative Tweets were more than positive ones in English and French networks, especially in French networks. It may be caused by people’s dissatisfaction with the government’s current carbon neutrality policy. By analysing the social pattern on Twitter, the research results allow people to know more about the means to enhance carbon-neutral knowledge sharing, which has the policy and social significance for addressing climate change.
The sense of agency in emerging technologies for human–computer integration: A review

Human–computer integration is an emerging area in which the boundary between humans and technology is blurred as users and computers work collaboratively and share agency to execute tasks. The sense of agency (SoA) is an experience that arises by a combination of a voluntary motor action and sensory evidence whether the corresponding body movements have somehow influenced the course of external events. The SoA is not only a key part of our experiences in daily life but also in our interaction with technology as it gives us the feeling of “I did that” as opposed to “the system did that,” thus supporting a feeling of being in control. This feeling becomes critical with human–computer integration, wherein emerging technology directly influences people’s body, their actions, and the resulting outcomes. In this review, we analyse and classify current integration technologies based on what we currently know about agency in the literature, and propose a distinction between body augmentation, action augmentation, and outcome augmentation. For each category, we describe agency considerations and markers of differentiation that illustrate a relationship between assistance level (low, high), agency delegation (human, technology), and integration type (fusion, symbiosis). We conclude with a reflection on the opportunities and challenges of integrating humans with computers, and finalise with an expanded definition of human–computer integration including agency aspects which we consider to be particularly relevant. The aim this review is to provide researchers and practitioners with guidelines to situate their work within the integration research agenda and consider the implications of any technologies on SoA, and thus overall user experience when designing future technology.
Portugal nautical stations: Strategic alliances for sport tourism and environmental sustainability

Nautical tourism is a tourist product with great development in the European space and a lot of potential to promote and develop tourist destinations. Considering the dynamics of nautical tourism management and the importance of meeting the specificities of this market niche, the objective of this study was to analyse the strategic alliances for the development of the offer of nautical tourism products, namely the strategic goals and sustainable environmental practices adopted by their actors which integrated the strategic alliances in order to certificate a plethora of nautical stations in Portugal. Between September and December 2021, 17 Portuguese nautical stations' application forms were collected. Content analysis using Nvivo software was the technique used for data analysis. The results showed a multiplicity of strategic objectives associated with the strategic alliance established between the nautical stations. The strategic vision of nautical stations for the development of strategic alliances is built, firstly, on the objective of structuring the tourism offer, followed by increasing governance and promoting and marketing nautical tourism using the image of the destination. Based on these results, it is possible to infer the importance of nautical stations in destination competitiveness and the role of strategic alliances in facilitating penetration in the nautical tourism market. The managers of nautical stations should consider the use of strategic alliances to make a cooperative marketing in order to improve the experience of the clients. Regarding environmental sustainability practices, the results exposed the prominence of environmental education actions in contrast to the reduced number of nautical stations developing actions for the adoption of sustainable transport. This study contributes to a better understanding of nautical tourism and Portuguese nautical stations, a project of strategic investment in sport and tourism, inferring on the objectives underlying the formation of strategic alliances and on the adopted environmental sustainability practices. The conclusions of this study point to the need for future scientific research on the actual operationalization of the objectives underlying the formation of strategic alliances, as well as the environmental practices developed by nautical stations.
Single-cell transcriptome analysis reveals T-cell exhaustion in denosumab-treated giant cell tumor of bone

Denosumab (DMAB), a human monoclonal antibody against the receptor activator of the nuclear factor-kappa B ligand, is used for the treatment for unresectable giant cell tumor of bone (GCTB). However, little is known about the molecular and functional characteristics of GCTB-infiltrating lymphocytes after DMAB treatment. Here, we performed single-cell RNA sequencing and immunostaining assays to delineate the immune landscape of GCTB in the presence and absence of DMAB. We found that exhausted CD8+ T cells were preferentially enriched in DMAB-treated GCTB. A distinct M2-skewed type of tumor-associated macrophages (TAMs) comprises the majority of GCTB TAMs. We identified cytokines, including interleukin-10, and inhibitory receptors of M2 TAMs as important mediators of CD8+ T cell exhaustion. We further revealed that DMAB treatment notably increased the expression levels of periostin (POSTN) in GCTB cells. Furthermore, POSTN expression was transcriptionally regulated by c-FOS signaling and correlated with GCTB recurrence in patients after DMAB treatment. Collectively, our findings reveal that CD8+ T-cells undergo unappreciated exhaustion during DMAB therapy and that GCTB cell-derived POSTN educates TAMs and establishes a microenvironmental niche that facilitates GCTB recurrence.
Dietary consumption of selenium inversely associated with osteoporosis in postmenopausal women

Background Osteoporosis is a skeletal disease characterized by reduced bone mineral density (BMD), which increases the risk of falls and fractures and reduces mobility. Some nutrients have a well-established role in maintaining bone health and preventing osteoporosis, while selenium (Se) has aroused interest in bone health possibly because of its anti-inflammatory and antioxidant capacity. The aim of this study was to evaluate the association between dietary Se consumption and BMD in postmenopausal women. Materials and methods Cross-sectional, observational, analytical study carried out with women in menopause for at least 12 months, aged ≥ 50 years. Sociodemographic, lifestyle, and clinical data variables were studied. BMD was assessed using Dual Energy X-ray Absorptiometry (DXA) and the participants classified as having normal BMD, osteopenia, or osteoporosis. Dietary consumption of Se was assessed by the food frequency questionnaire (FFQ) and classified into quartiles of consumption. Multivariate logistic regression with three fit models was applied to investigate the association of BMD with Se consumption quartiles. The significance level adopted for all tests was 5.0%. Results The final sample consisted of 124 women aged in average 66.8 ± 6.1 years and with a time since menopause of 19.6 ± 8.8 years. According to the BMD, 41.9% of the women had osteopenia and 36.3% osteoporosis. The mean consumption of Se was 154.4 ± 88.7 μg/day. The highest consumption of Se was observed among women with normal BMD (51.9%), whereas lower consumption levels were found in 57.7% of women with osteopenia and in 60.0% of women with osteoporosis (p = 0.003). In the multivariate analysis, after adjusting for possible confounding variables, Se remained associated with the group of women with osteoporosis. Postmenopausal women in the highest quartile (≥94.0 μg/day) of Se consumption had an OR of 0.02 (95%CI: 0.001–0.41; p = 0.012) of having osteoporosis when compared with women in the lowest quartile. Conclusion Se consumption was associated with BMD and postmenopausal women with higher Se consumption were less likely to have osteoporosis.
Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate

Pax3/7 factors play numerous roles in the development of the dorsal nervous system of vertebrates. From specifying neural crest at the neural plate borders, to regulating neural tube closure and patterning of the resulting neural tube. However, it is unclear which of these roles are conserved in non-vertebrate chordates. Here we investigate the expression and function of Pax3/7 in the model tunicate Ciona. Pax3/7 is expressed in neural plate border cells during neurulation, and in central nervous system progenitors shortly after neural tube closure. We find that separate cis-regulatory elements control the expression in these two distinct lineages. Using CRISPR/Cas9-mediated mutagenesis, we knocked out Pax3/7 in F0 embryos specifically in these two separate territories. Pax3/7 knockout in the neural plate borders resulted in neural tube closure defects, suggesting an ancient role for Pax3/7 in this chordate-specific process. Furthermore, knocking out Pax3/7 in the neural impaired Motor Ganglion neuron specification, confirming a conserved role for this gene in patterning the neural tube as well. Taken together, these results suggests that key functions of Pax3/7 in neural tube development are evolutionarily ancient, dating back at least to the last common ancestor of vertebrates and tunicates.
Probing oxygen activation on plasmonic photocatalysts

In this work we present an assay to probe the oxygen activation rate on plasmonic nanoparticles under visible light. Using a superoxide-specific XTT molecular probe, the oxygen activation rate on bimetallic gold-silver “rainbow” nanoparticles with a broadband visible light (> 420 nm) response, is determined at different light intensities by measuring its conversion into the colored XTT-formazan derivate. A kinetic model is applied to enable a quantitative estimation of the rate constant, and is shown to match almost perfectly with the experimental data. Next, the broadband visible light driven oxygen activation capacity of this plasmonic rainbow system, supported on nano-sized SiO2, is demonstrated towards the oxidation of aniline to azobenzene in DMSO. To conclude, a brief theoretical discussion is devoted to the possible mechanisms behind such plasmon-driven reactions.
Editorial: Mechanical ventilation in anesthesia and critical care small animal patients

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Structural and mechanical properties of the red blood cell’s cytoplasmic membrane seen through the lens of biophysics

Red blood cells (RBCs) are the most abundant cell type in the human body and critical suppliers of oxygen. The cells are characterized by a simple structure with no internal organelles. Their two-layered outer shell is composed of a cytoplasmic membrane (RBC cm ) tethered to a spectrin cytoskeleton allowing the cell to be both flexible yet resistant against shear stress. These mechanical properties are intrinsically linked to the molecular composition and organization of their shell. The cytoplasmic membrane is expected to dominate the elastic behavior on small, nanometer length scales, which are most relevant for cellular processes that take place between the fibrils of the cytoskeleton. Several pathologies have been linked to structural and compositional changes within the RBC cm and the cell’s mechanical properties. We review current findings in terms of RBC lipidomics, lipid organization and elastic properties with a focus on biophysical techniques, such as X-ray and neutron scattering, and Molecular Dynamics simulations, and their biological relevance. In our current understanding, the RBC cm ’s structure is patchy, with nanometer sized liquid ordered and disordered lipid, and peptide domains. At the same time, it is surprisingly soft, with bending rigidities κ of 2–4 kBT. This is in strong contrast to the current belief that a high concentration of cholesterol results in stiff membranes. This extreme softness is likely the result of an interaction between polyunsaturated lipids and cholesterol, which may also occur in other biological membranes. There is strong evidence in the literature that there is no length scale dependence of κ of whole RBCs.