Miracle-dz/UniFAR-FTUs huggingface dataset:
Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells

Adult human cardiac mesenchymal progenitor cells (hCmPC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Even if the mechanisms have not yet been fully elucidated, the stem cell differentiation is guided by the mitochondrial metabolism; however, mitochondrial approaches to identify hCmPC with enhanced stemness and/or differentiation capability for cellular therapy are not established. Here we demonstrated that hCmPCs sorted for low and high mitochondrial membrane potential (using a lipophilic cationic dye tetramethylrhodamine methyl ester, TMRM), presented differences in energy metabolism from preferential glycolysis to oxidative rates. TMRM-high cells are highly efficient in terms of oxygen consumption rate, basal and maximal respiration, and spare respiratory capacity compared to TMRM-low cells. TMRM-high cells showed characteristics of pre-committed cells and were associated with higher in vitro differentiation capacity through endothelial, cardiac-like, and, to a lesser extent, adipogenic and chondro/osteogenic cell lineage, when compared with TMRM-low cells. Conversely, TMRM-low showed higher self-renewal potential. To conclude, we identified two hCmPC populations with different metabolic profile, stemness maturity, and differentiation potential. Our findings suggest that metabolic sorting can isolate cells with higher regenerative capacity and/or long-term survival. This metabolism-based strategy to select cells may be broadly applicable to therapies.
Épidémiologie moléculaire de la mucoviscidose en Tunisie

La mucoviscidose ou fibrose kystique du pancreas est la maladie autosomique recessive la plus frequente dans la population nord europeenne. Le developpement des techniques de biologie moleculaire a largement facilite la mise en place des techniques d’etude des differentes lesions moleculaires du gene CFTR responsables de la mucoviscidose dont le nombre actuellement est superieur a 1 300 mutations. Dans le present travail, nous rapportons la strategie d’etude de ces lesions sur 390 enfants appartenant a 383 familles non apparentees suspects de mucoviscidose et qui nous sont parvenues de la plupart des regions de la Tunisie. Plusieurs techniques de biologie moleculaire ont ete utilisees pour le diagnostic genotypique : l’extraction de l’ADN a partir du sang peripherique, la reaction de polymerisation en chaine (PCR), l’electrophorese sur gel de polyacrylamide, la PCR multiplex et l’electrophorese sur gel en gradient denaturant (DGGE) suivie au besoin par une reaction de sequencage du gene CFTR. Cette etude a permis d’identifier 17 mutations localisees sur differents exons du gene CFTR. La mutation deletionnelle F508del est la plus frequente (50,74 %) suivie de 3 autres mutations communement retrouvees dans les pays du bassin mediterraneen : G542X, W1282X et N1303K, a cote de 4 autres mutations (T665S, 2766del8, F1166C, L1043R) retrouvees exclusivement a ce jour, dans la population tunisienne. Les resultats obtenus ont permis d’une part, d’etablir le profil des mutations mucoviscidosiques et leur distribution dans le pays et, d’autre part, de mettre en œuvre une strategie de prevention de ces maladies a travers le conseil genetique et le diagnostic prenatal.
Wavoice: A mmWave-assisted Noise-resistant Speech Recognition System

As automatic speech recognition evolves, the deployment of voice user interface has boomingly expanded. Especially since the COVID-19 pandemic, VUI has gained more attention in online communication owing to its non-contact property. However, VUI struggles to be applied in public scenes due to the degradation of received audio signals caused by various ambient noises. In this paper, we propose Wavoice, the first noise-resistant multi-modal speech recognition system that fuses two distinct voice sensing modalities, i.e., millimeter-wave (mmWave) signals and audio signals from a microphone, together. One key contribution is to model the inherent correlation between mmWave and audio signals. Based on it, Wavoice facilitates the real-time noise-resistant voice activity detection and user targeting from multiple speakers. Additionally, we elaborate on two novel modules for multi-modal fusion embedded into the neural network, leading to accurate speech recognition. Extensive experiments prove the effectiveness of Wavoice under adverse conditions, that is, the character recognition error rate below 1 \(\% \) in a range of 7 meters. In terms of robustness and accuracy, Wavoice considerably outperforms existing audio-only speech recognition methods with lower character error rate and word error rate.
Investigation of Dental and Oral Health in Children and Adolescents with Special Support Needs from a Child and Adolescent Psychiatric Perspective

Background: The current literature lacks scientific research on child and adolescent psychiatrists’ (CAPPS) perspectives on dental and oral health. This study aims to investigate the opinions and approaches of child and adolescent psychiatrists and their patients regarding oral and dental health. Methods: A questionnaire-based cross-sectional study was conducted among members of the Professional Association for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy in Germany. Results: Out of the association members, 10.9% (n = 109) participated, with 5.2% (n = 52; 38f/14m) completing the questionnaire. Dental and oral health topics were discussed with one-fifth of the patients (19.2%), while 11.5% reported that they were “never” a part of their therapy. Patient-related concerns about dental and oral health were primarily brought into the context of child and adolescent psychiatric work. Dental treatment anxieties were prominent. Only 3.8% of the participants regularly assigned diagnoses related to dental status. The CAPPS employ a bio-psycho-social model for the genesis of oral health-related conditions in Children and Adolescents with Special Needs. Conclusions: CAPPS have a foundation in relationship-based work for assessing oral and dental healthcare and providing recommendations for further dental care. Regional networking and science must be further developed.
Carotenoid coloration and health status of urban Eurasian kestrels (Falco tinnunculus)

As the world experiences rapid urban expansion, natural landscapes are being transformed into cities at an alarming rate. Consequently, urbanization is identified as one of the biggest environmental challenges of our time, yet we lack a clear understanding of how urbanization affects free-living organisms. Urbanization leads to habitat fragmentation and increased impervious surfaces affecting for example availability and quality of food. Urbanization is also associated with increased pollution levels that can affect organisms directly, via ecophysiological constraints and indirectly by disrupting trophic interactions in multi-species networks. Birds are highly mobile, while an individual is not necessarily exposed to urban stressors around the clock, but nestlings of altricial birds are. Such a city-dwelling species with a long nestling phase is the Eurasian kestrel (Falco tinnunculus) in Vienna, Austria, which forage on a diverse diet differing in composition from rural habitats. Furthermore, prey items vary in nutritional value and contents of micronutrients like carotenoids, which might impact the nestlings’ health. Carotenoids are pigments that are incorporated into integument tissues but also have antioxidant and immunostimulatory capacity, resulting in a trade-off between these functions. In nestlings these pigments function in parent-offspring communication or sibling competition by advertising an individual’s physical or physiological condition. Anthropogenic disturbance and pollutants could have disruptive effects on the coloration of these traits. In this study, we measured carotenoid based coloration and other indicators of individual health (body condition and susceptibility to the ectoparasite Carnus hemapterus) of 154 nestling kestrels (n = 91 nests) along an urban gradient from 2010 to 2015. We found skin yellowness of nestlings from nest-sites in the city-center to be least pronounced. This result might indicate that inner-city nestlings are strongly affected by urban stressors and depleted their stores of dietary carotenoids for health-related functions rather than coloration. In addition, skin yellowness intensified with age and was stronger pronounced in earlier nests. Since the immune system of nestlings is still developing, younger chicks might need more antioxidants to combat environmental stress. Additionally, parasite infection intensity was highest in nestlings with less intense skin yellowness (paler or less yellow pigmented integuments) and in earlier nests of the season. In combination with results from previous studies, our findings provide further support for the low quality of the inner-city habitat, both in terms of productivity and individual health.
Baby boom consumers and technology: shooting down stereotypes

Purpose – Current stereotypes of aging consumers paint a picture of them as technology anxious and reluctant to adopt new technologies. This paper aims to show that the present 50‐to‐60 age group does not fit these stereotypes.Design/methodology/approach – A total of 620 Finnish baby boomers (aged between 50 and 60) responded to a mail survey.Findings – Baby boom consumers are shown, in contrast to the stereotypes, to have low levels of technology anxiety and high levels of experience of internet and SMS usage. It is also shown that technology anxiety and experience have an inverse relationship in the age group.Research limitations/implications – To some extent, the results provide evidence of the distortedness of current stereotypes that are used to describe 50‐plus consumers. However, data was gathered only in one country. Replications of this study in different countries would allow drawing broader conclusions.Practical implications – Marketers of technological products and services are advised to let .
Neuropeptide Y reduces expression of social fear via simultaneous activation of Y1 and Y2 receptors

Background: Neuropeptide Y (NPY) has anxiolytic effects and facilitates extinction of cued and contextual fear in rodents, thereby acting as a resilience factor against exaggerated fear responses after adverse events. We investigated whether NPY influences acquisition, expression and extinction of social fear in a mouse model of social fear conditioning (SFC). Methods: NPY was administered intracerebroventricularly before SFC or before social fear extinction with or without prior administration of Y1 and/or Y2 receptor antagonists. Results: We show that NPY affects SFC-induced social fear in a time point–dependent manner. When administered before SFC, NPY did not affect acquisition, expression and extinction of social fear. However, when administered before social fear extinction, NPY reduced expression of social fear via simultaneous activation of Y1 and Y2 receptors. As such, neither the Y1 receptor antagonist BIBO3304 trifluoroacetate nor the Y2 receptor antagonist BIIE0246 was able to block the effects of NPY completely. However, when administered in combination, they completely blocked the effects of NPY on social fear expression. Conclusions: These findings have important clinical implications, as they suggest that although medication strategies aimed at increasing brain NPY activity are unlikely to prevent the formation of aversive memories after a traumatic social experience, they might improve the recovery from a traumatic social experience by reducing the expression of social fear.
Maintenance of Specificity in an Isolated Fig

The obligate interaction between figs and their pollinating wasps is often cited as an extraordinary example of reciprocal species specificity and evolutionary cospeciation. However, recent studies have shown that breakdowns in one‐to‐one specificity are not rare (30–60% of species depending on the locality). Combined with evidence of hybridization in some species, this led researchers to propose that a better evolutionary model was one of groups of genetically well‐defined pollinators coevolving with groups of frequently hybridizing figs. Nevertheless, these recent studies still indicate that a majority of fig species have one or more host‐specific pollinator. The extent to which specificity barriers in these species are leaky will have important consequences for the evolutionary process in Ficus. At Lambir Hills N.P., Sarawak, a single individual of Ficus acamptophylla has become recently isolated from conspecifics through clearance of its specialized habitat, but adjacent forest has a diverse fig flora, including 16 species with congeneric pollinators. Thus, when this individual flowered I was able to investigate the maintenance of its specificity barriers in the absence of competition from the normal pollinator. Only 1 percent of inflorescences were entered by a single pollinator species, which had very low reproductive success, and no viable seeds were produced. Nonpollinating wasps also failed to reproduce in any of the inflorescences. These results indicate the maintenance of strict specificity barriers in this fig individual.
Mapping normal and cancer cell signalling networks: towards single-cell proteomics

Abstract | Oncogenesis and tumour progression are supported by alterations in cell signalling. Using flow cytometry, it is now possible to track and analyse signalling events in individual cancer cells. Data from this type of analysis can be used to create a network map of signalling in each cell and to link specific signalling profiles with clinical outcomes. This form of ‘single-cell proteomics’ can identify pathways that are activated in therapy-resistant cells and can provide biomarkers for cancer diagnosis and for determining patient prognosis. Alterations in signalling result in increased survival and proliferation of cancer cells, as well as increasing immune evasion by cancer cells. Although a wide variety of genetic and epigenetic events contribute to these alterations, it has been challenging to gain an overall picture of the common effects that these changes have on the entire signalling network. A ‘network-level’ view of signalling in normal and cancer cells is therefore needed to identify shared features of malignant cells. Flow cytometry, which simultaneously quantifies multiple properties of individual cells, is well suited to this task because a ‘map’ of the signalling network can be derived for each cell in a mixed population and compared with other cellular features. This ‘single-cell resolu-data can be used to distinguish signalling maps of cancer cells from non-tumour cells present in patient samples. It is also possible to identify cancer cell subsets based on their signalling maps. By relating changes in cancer signalling networks to patient out-come, cancer cell signalling can be used to
Factorization of derivatives of Bloch mappings through a Hilbert space

We introduce the concept of vector-valued holomorphic mapping on the complex unit disc whose derivative factors through a Hilbert space and state the main properties of the space formed by such Bloch mappings equipped with a natural norm: linearization, Bloch transposition, surjective and injective Banach ideal property, invariance by M\"obius transformations, Kwapi\'en-type characterization by Bloch domination, and duality.
The Impact of Perceived Value and Attitude towards Purchase Intention at Sambel Branding Restaurant

Sambal is a traditional Indonesian spice that has been an essential component of Indonesian cuisine for centuries. Today, it is considered a staple spice in all Indonesian dishes. The popularity of sambal has attracted many new restaurants of varying scales to enter the spicy food market and utilize the “Sambel” brand as their restaurant attraction. Jakarta, which is one of the eight primary cities in Indonesia, ranks fourth with the most restaurant dine-in visits in 2022. As per the data provided by the Central Statistics Agency (BPS), there are 6,780 restaurant units in Indonesia of medium to large scale. Jakarta secures the top spot as the province with the most medium-scale restaurants in Indonesia, with 3,062 units in 2021. Sambel Korek DNO is one of the dining places in Jakarta with the Sambel, the central outlet is located in Sumur Batu Kemayoran. This establishment is the highest contributor to the sales of Sambel Korek DNO as compared to other establishments. The significant consumption levels of Jakartans and their fondness for spicy cuisine drive new entries to launch their street food stalls, small establishments, and restaurants under the Sambel brand, causing an increase in competition. However, the sales of DNO Korek Sambal did not meet the target this year due to a decline in the number of customers dining at this outlet. This could be attributed to low brand awareness, as many other eateries also use the “Sambel” branding. Additionally, customers’ perception of quality may be influenced by the similarity in offerings with other major players in the “Sambel” market, such as Waroeng SS and Sambal Bakar Indonesia. This research wants to analyze whether perceived value and attitude could mediate brand awareness and perceived quality toward purchase intention for potential customers and existing customers. This research is conducted using SPSS and PLS-SEM. Resulting that Attitude has no mediating effects on brand awareness and perceived quality towards purchase intention. However, perceived quality has mediating effects on brand awareness and perceived quality towards attitude, which has a significant influence on purchase intention.
Student Perception as a Planning Input in a Project-Based Construction Program

This paper addresses the results of the first stage of a study that explores different factors impacting students’ perception in a construction program that presents content through an innovative curricular model—studio-based learning. While there are disciplines outside of those that traditionally embrace studio-based learning such as architecture, art, and design, the application of this method to construction is limited. The similarity of learning objectives such as critical thinking, problem-solving, and collaboration across disciplines to produce buildings provide a strong foundation for applying studio-based learning to construction. While many aspects of architecture studios can be translated and implemented into the construction curriculum, neglecting inherent features of construction education endangers the potential to maximize the potential of this innovative curricular model to achieve effective construction learning. These features include physical, structural, and cognitive aspects of construction curricula necessitated by studio-based learning. In this environment, student perception and feedback can be utilized as a valuable input to regulate the learning system and increase its effectiveness. This paper summarizes quantitative research that was conducted in fall 2017 to investigate how different aspects of studio impact students’ perception. This paper explores how students perceive the time structure and layout of the studio space. The results indicate that students perceive various aspects of studio positively, however, their perceptions toward some factors may vary by academic class level. These results are significant because they indicate that students perceive different layouts of studio differently. Modifying the planning and organization of studios ensure that the learning methods and environment support the objectives of the program.
A New Approach of copy move Forgery Detection using Rigorous Preprocessing and Feature Extraction

— these days, advanced pictures are being used in an extensive variety of uses and for numerous reasons. They additionally assume an imperative part in the capacity and exchange of visual data, particularly the mystery ones. With this far reaching utilization of advanced pictures, notwithstanding the expanding number of devices and programming of computerized pictures altering, it has turned out to be anything but difficult to control and change the real data of the picture. In this way, it has turned out to be important to check the credibility and the respectability of the picture by utilizing present day and advanced methods, which add to examination and comprehension of the pictures’ substance, and after that ensure their trustworthiness. There are many sorts of picture imitation, the most critical and prominent sort is called duplicate glue fabrication, which utilizes a similar picture during the time spent falsification. This sort of fraud is utilized for one of two things, first to cover a protest or scene by replicating the region of the picture and gluing it on another zone of a similar picture. In this paper we have presented a new approach of copy move forgery detection. proposed scheme uses Oriented FAST and rotated BRIEF(ORB) alternative of scale invariant feature transform (SIFT) technique which is integrated with modified local contrast modification-contrast limited adaptive histogram equalization(LCM-CLAHE). Experimental results shows that proposed scheme is more promising in terms of false positive rate(FPR) and true positive rate(TPR) compare to state of the art techniques.
Insights into the Mitochondrial and Nuclear Genome Diversity of Two High Yielding Strains of Laying Hens

Simple Summary Mitochondria are commonly known as “the powerhouse of the cell”, influencing the fitness, lifespan and metabolism of eukaryotic organisms. In our study we examined mitochondrial and nuclear genomic diversity in two high yielding strains of laying hens. We tested if the mitochondrial genome affects functional traits such as body weight and phosphorus utilization. We discovered a surprisingly low mitochondrial genetic diversity and an unequal distribution of the haplotypes among both strains, leading to limitations of robust links to phenotypic traits. In contrast, we found similar levels of nuclear genome diversity in both strains. Our study explores the potential influence of the mitochondrial genome on phenotypic traits and thus contributes to a better understanding of the function of this organelle in laying hens. Further, we focus on its usefulness as a genetic marker, which is often underestimated in breeding approaches, given the different inheritance mechanism compared to the nuclear genome. Abstract Mitochondria are essential components of eukaryotes as they are involved in several organismic key processes such as energy production, apoptosis and cell growth. Despite their importance for the metabolism and physiology of all eukaryotic organisms, the impact of mitochondrial haplotype variation has only been studied for very few species. In this study we sequenced the mitochondrial genome of 180 individuals from two different strains of laying hens. The resulting haplotypes were combined with performance data such as body weight, feed intake and phosphorus utilization to assess their influence on the hens in five different life stages. After detecting a surprisingly low level of genetic diversity, we investigated the nuclear genetic background to estimate whether the low mitochondrial diversity is representative for the whole genetic background of the strains. Our results highlight the need for more in-depth investigation of the genetic compositions and mito-nuclear interaction in individuals to elucidate the basis of phenotypic performance differences. In addition, we raise the question of how the lack of mitochondrial variation developed, since the mitochondrial genome represents genetic information usually not considered in breeding approaches.