Miracle-dz/UniFAR-FTUs huggingface dataset:
N-Terminus Plays a Critical Role for Stabilizing the Filamentous Assembly and the Antifungal Activity of Bg_9562

Burkholderia gladioli strain NGJ1, isolated from healthy rice seedling, was earlier demonstrated to have mycophagous properties on a broad range of fungi, including Rhizoctonia solani, a causal agent of deadly sheath blight disease of rice. The purified Bg_9562 protein exerts broad-spectrum antifungal activity. ABSTRACT Bg_9562, a prophage tail-like protein was earlier shown to be required for bacterial mycophagy by Burkholderia gladioli strain NGJ1. The purified protein exhibited broad-spectrum antifungal activity; however, the structural and mechanistic details vis-à-vis its activity remained elusive. In this study, we have structurally characterized the protein Bg_9562 using negatively stained transmission electron microscopy, molecular modeling and mutagenesis. We find that Bg_9562 shows structural similarity to Gp13, a tail assembly chaperone. The transmission electron microscopy revealed that, Bg_9562 forms long flexible tubular structures. Molecular modeling of the filament like structure divulges that the inter subunit contacts are meditated largely through hydrophobic interactions. Using mutagenesis, we demonstrate that the N-terminal residues of the protein when deleted results in reduced activity and destabilization of filament formation. Overall, structure-function analysis opens up avenues for further utilization of the protein as a potent antifungal molecule. IMPORTANCE Burkholderia gladioli strain NGJ1, isolated from healthy rice seedling, was earlier demonstrated to have mycophagous properties on a broad range of fungi, including Rhizoctonia solani, a causal agent of deadly sheath blight disease of rice. The purified Bg_9562 protein exerts broad-spectrum antifungal activity. The protein also inhibits the growth of laboratory strain of Candida, an opportunistic human pathogen. In this study, we structurally characterize Bg_9562 using a combination of negative staining transmission electron microscopy, molecular modeling, mutagenesis, and functional antifungal assay. We show that the protein assembles into long filament like structures stabilized by N-terminus residues and this region is important for its activity. Our study has implications in utilizing Bg_9562 or its derivatives as antifungal molecule(s) which will provide environmentally friendly control of fungal diseases of plants and animals.
Alu hypomethylation in naturally and surgically postmenopausal women; a cross-sectional study

Menopause, which may accelerate the hallmarks of the natural aging process, represents a point in time characterized by the permanent cessation of menstruation following the loss of ovarian estrogen production. Unlike natural menopause, which is characterized by a gradual decrease in estrogen production, when both ovaries are removed before the natural age of menopause, the onset of estrogen deprivation is abrupt. Further, a decrease in genome methylation frequently occurs in aging cells, and the major interspersed repetitive DNA elements in humans are Alu elements. In blood cells, Alu demethylation starts at an age of approximately 40 years, and increases with age. Here, we explored the Alu methylation levels corresponding to age-matched pre-menopausal, naturally postmenopausal, and surgically postmenopausal women aged 45–55 years (n = 60 in each group). Our results indicated that the body mass index (BMI), time-since-menopause, and Alu methylation levels corresponding to the three groups were significantly different. However, no correlations between Alu methylation level and BMI, time-since-menopause, or age were observed. Additionally, the Alu methylation level corresponding to the natural post-menopause group was significantly lower those corresponding to the pre-menopausal (p = 0.001) and surgical post-menopausal (p = 0.037) groups. In conclusion, Alu hypomethylation occurs in naturally postmenopausal women, implying that when women reach the age of natural menopause, the cell aging process may progress significantly with genome hypomethylation. These findings, notwithstanding, further studies are necessary to clarify whether bilateral oophorectomy before the age of menopause affects the cell aging process to a greater extent than natural menopause, and whether estrogen therapy or other interventions can delay cell aging in this regard.
Primary school children rate children with autism negatively on looks, speech and speech content.

Adults and adolescents form negative first impressions of ASD adults and children. We examined the first impression ratings of primary school children (6-9 years) of their ASD peers. 146 school children rated either silent videos, speech or transcribe speech from 14 actors (7 ASD, 7 TD). The ASD actors were rated more negatively than the typically developing actors on all three stimulus types. Children with ASD are likely to be judged more negatively than their peers at the very start of their formal education. Contrary to previous research, for primary school children, the content of the speech was judged as negatively as the delivery of the speech.
Deconfounding serial recall: Response timing and the overarching role of grouping.

We used the timing of serial recall in several situations to reveal important aspects of recall groupings that participants construct and the reasons those groupings occur. We examined the timing of responses in the recall of digit strings within two published experiments. Cowan, Saults, Elliott, and Moreno (2002) examined memory for nine-item lists in a way that deconfounded the presentation modality, input versus output serial position (using a varied starting point of recall), memory load from items not yet recalled (using whole vs. partial recall), and the presence or absence of the temporal grouping of the lists into triads. Accuracy was strikingly different in the two modalities, with grouping drastically changing recall of acoustic lists but with little difference between grouped versus ungrouped visual lists. Despite the modality difference in recall, the present timing results show that participants imposed a similar grouped structure in recall for both modalities. When errors were made, the original grouping structure still was maintained in the output timing. Cowan et al. (2005, Experiment 1) presented ungrouped acoustic lists of varying lengths in a span task. In this case, there was never a grouping structure presented to the participant and the accuracy data did not reveal evidence of grouping, but we show clear grouping in the timing structure. This grouping structure varied with both the list length and an individual's span. Timing in both experiments shows that a grouping structure is imposed on responses in a manner that is dependent on several factors not present in the stimuli. (PsycInfo Database Record (c) 2022 APA, all rights reserved).
Addressing inequity in palliative care provision for older people living with multimorbidity. Perspectives of community-dwelling older people on their palliative care needs: A scoping review

Background: Older people living with multimorbidity are projected to become the main recipients of palliative care in the coming decades, yet there is limited evidence regarding their expressed palliative care needs to inform person-centred care. Aim: To understand the palliative care needs of community-dwelling people aged ⩾60 living with multimorbidity in the last 2 years of life. Design: A scoping review following Arksey and O’Malley. Data sources: Three international electronic databases (CINAHL, Ovid Medline, PsycINFO) were searched from March 2018 to December 2021. Reference lists were hand searched. Eligible papers were those reporting empirical data on older people’s needs. Results: From 985 potential papers, 28 studies were included, published between 2002 and 2020; sixteen quantitative, nine qualitative and three mixed methods. Data were extracted and presented under the holistic palliative care domains of need: physical, psychological, social, spiritual, and additionally practical needs. Different measurement tools (n = 29) were used, of which 20 were multidimensional. Primacy in reporting was given to physical needs, most commonly pain and function. Social and practical needs were often prioritised by older people themselves, including maintaining social connections and accessing and receiving individualised care. Conclusion: Identifying the palliative care needs that matter most to older people with multimorbidity requires the recognition of their concerns, as well as their symptoms, across a continuum of living and dying. Available evidence is superficial. Supporting end of life provision for this growing and underserved population necessitates a shift to tailored multidimensional tools and community focussed integrated care services.
The Relationship between Parental Monitoring, Peer Pressure, and Motivations for Responsible Drinking among Italian Adolescents: The Mediating Role of Positive Alcohol Expectancies

Abstract This study investigated the associations between parental monitoring, peer pressure, and motivations for responsible drinking, while also taking the mediating role of positive alcohol expectancies into account. The participants were 579 Italian adolescents, aged 14-20 years (M = 16.39 years, SD = 1.27; 55.3% females), involved in a cross-sectional survey. They were administered online self-report questionnaires. Structural equation modeling revealed both direct and indirect positive associations between study variables. Parental monitoring was positively associated, both directly and indirectly, with adolescents’ motivations for responsible drinking through the mediation of positive alcohol expectancies; peer pressure was negatively and indirectly associated with adolescents’ motivations for responsible drinking, via the mediating role of positive alcohol expectancies. Findings highlighted the importance of environmental factors with regard to motivations for responsible drinking, suggesting the opportunity to implement prevention programs to improve parental monitoring and increase adolescents’ skills to manage peer pressure and to develop realistic expectancies about drinking.
A latent state space model for estimating brain dynamics from electroencephalogram (EEG) data

Modern neuroimaging technologies have substantially advanced the measurement of brain activity. Electroencephalogram (EEG) as a noninvasive neuroimaging technique measures changes in electrical voltage on the scalp induced by brain cortical activity. With its high temporal resolution, EEG has emerged as an increasingly useful tool to study brain connectivity. Challenges with modeling EEG signals of complex brain activity include interactions among unknown sources, low signal‐to‐noise ratio, and substantial between‐subject heterogeneity. In this work, we propose a state space model that jointly analyzes multichannel EEG signals and learns dynamics of different sources corresponding to brain cortical activity. Our model borrows strength from spatially correlated measurements and uses low‐dimensional latent states to explain all observed channels. The model can account for patient heterogeneity and quantify the effect of a subject's covariates on the latent space. The EM algorithm, Kalman filtering, and bootstrap resampling are used to fit the state space model and provide comparisons between patient diagnostic groups. We apply the developed approach to a case‐control study of alcoholism and reveal significant attenuation of brain activity in response to visual stimuli in alcoholic subjects compared to healthy controls.
Measuring the impact of social-distancing, testing, and undetected asymptomatic cases on the diffusion of COVID-19

The key to overcoming COVID-19 lies, arguably, in the diffusion process of confirmed cases. In view of this, this study has two main aims: first, to investigate the unique characteristics of COVID-19—for the existence of asymptomatic cases—and second, to determine the best strategy to suppress the diffusion of COVID-19. To this end, this study proposes a new compartmental model—the SICUR model—which can address undetected asymptomatic cases and considers the three main drivers of the diffusion of COVID-19: the degree of social distancing, the speed of testing, and the detection rate of infected cases. Taking each country’s situation into account, it is suggested that susceptible cases can be classified into two categories based on their sources of occurrence: internal and external factors. The results show that the ratio of undetected asymptomatic cases to infected cases will, ceteris paribus, be 6.9% for South Korea and 22.4% for the United States. This study also quantitatively shows that to impede the diffusion of COVID-19: firstly, strong social distancing is necessary when the detection rate is high, and secondly, fast testing is effective when the detection rate is low.
UWB Technique for Detection of Live Buried Persons under Debris

- In this paper, a theoretical model for the propagation of ultra-wideband radar signals through some of the common types of building materials covering a human victim during collapse of a building or an earthquake is presented. The wave propagation attributes such as signal reflection, transmission and attenuation are calculated by following the analytical impedance transformation approach on a planar multilayered dispersive model in the frequency range of 1 to 3 GHz using MATLAB. The difference in the above propagation characteristic parameters when the radar beam is focused atop the pile of the building debris is truly the index of the life sign. The model predicted results will enable efficient power budget estimation for an UWB radar based life detection system.
Effects of military training on the bone density and bone metabolism index of the army recruits

[Abstract] Objective To evaluate the effects of a 3-month military training on the bone mineral density (BMD) and serum bone metabolism indexes of the army recruits. Methods A total of 275 male Han recruits, aged 17-23 (19.3 ± 1.5) years, were involved in present study, and underwent a 3-month military training according to the 2009 edition of the "Outline of Military Training and Assessment". All the parameters were evaluated before and after the military training including height, weight, and body mass index (BMI). Dual energy X-ray absorptiometry (DXA) was employed to check the BMD of lumbar vertebra 1-4 (L 1-4 ), Left femoral neck (FN) and total hip (TH); Chemiluminescence method was used to detect the bone specific alkaline phosphatase (BALP) level in serum bone formation marker; and ELISA was performed to detect the anti-tartrate 5b (TRAP5b) levels in serum bone resorption markers. Results There's significant change of height, weight and BMI before and after training ( P < 0.05). r existed between the change ratio of TRACP5b and that of L 1-4 BMD ( r =0.045, P =0.724) and TH BMD ( r =–0.102, P =0.417). There's no significant correlation between the change ratio of FN BMD and that of serum BALP ( r =0.051, P =0.685) and TRACP5b ( r =–0.137, P =0.277). Conclusions Military training of army recruits may obviously promote the bone formation. BMD and serum bone metabolism indexes may be used to evaluate the bone health status of recruit.
CONSTRAINED JUMP-DIFFUSION PROCESSES

: We consider a class of jump-diﬁusion processes, constrained to a polyhedral cone G ‰ IR n , where the constraint vector ﬂeld is constant on each face of the boundary. The constraining mechanism corrects for \attempts" of the process to jump outside the domain. Under Lipschitz continuity of the Skorohod map ¡, it is known that there is a cone C such that the image ¡ ` of a deterministic linear trajectory ` remains bounded if and only if _ ` 2 C . Denoting the generator of a corresponding unconstrained jump-diﬁusion by L , we show that a key condition for the process to admit an invariant probability measure is that for x 2 G , L id( x ) belongs to a compact subset of C o .
Aspects of Human Development Job Satisfaction among Youth in Development Sector: A study on PGDRDM students of NIRDPR

Human resource managers of the present generation find it very difficult to ignore the concept of Job Satisfaction in a period when demand for meaningful work is on the rise. There is immense competition for scarce resources that resulted in increased labour cost. Organizations hence prefer to reduce labour turnover without having to hire more employees. This can be done only if the companies can retain the interests of existing employees. But job satisfaction is dependent upon a variety of factors more so in this digital era where youth trained in utilizing Information Communication Technology do have information about bright career opportunities. Youth in any sector, whether it is Information Technology, Manufacturing or development sector prefer to continue in their area of interest according to their level of Job satisfaction. The present paper focuses upon the job satisfaction levels of the present day youth in development sector. Through structured Questionnaire method 60 Post Graduate Diploma in Rural Development alumni students currently working in development sector have been assessed. The results indicated that youth in this digital era are having high expectations about their career and growth prospects and are ready to accept any kind of challenges.
Stage Performance and Musical Analysis of Haacaaluu Hundeessaa Works by using Sentiment Analysis Techniques

This research is initiated to analysis the stage performance and musical analysis of Haacaaluu Hundeessaa works on Afaan oromoo reviews text collected from social Medias by applying the concept of sentiment analysis techniques. The data (reviews) were collected manually from social media on Haacaaluu Hundeessaa musical works. Haacaaluu Hundeessaa is Oromo singer, songwriter, civil rights activist and his being a voice his people. He is the most famous young singer, hero and icon of Oromo musician. From his musical works Sanyii Mootii(Race of the King), Waa'ee Keenyaa(Our Plight), Maalan Jira(What existence is mine), JIRTUU? are the most popular known in Oromo peoples as well as nation and nationality of Ethiopia. From Hacaaluu musical works, the researcher considered reviews (opinions) afaan oromoo text data on his single sang “JIRTUU? ” to analysis stage performance of Haacaaluu Hundeessaa’s. The researcher inspires to use this single sang because; reviews (comments) are easier to access, available on you tube channel, extensive in size relatively from other his songs from social media. Additionally this song was broadcast live by Oromia Broadcasting Network and other Medias on different stage. Opinion lexicon approach with contextual valence shifter is used for analyzing opinions (comments) given on stage performance of Haacaaluu Hundeessaa works. Totally 18,687 reviews opinions or sentences were collected for experimentations to analysis stage performance and musical works of Haacaaluu Hundeessaa. Accordingly our result indicates around 99.52 percents peoples likes the stage performance and musical works of Hacaaluu Hundeessaa. From the results researcher observed Haacaaluu Hundeessaa has good performer with sing emotional and physically when he sing on the stages. In future works, further research needs to study others musical works of Hacaaluu Hundeessaa.
Effects of pattern systems on production performance and egg quality of laying hen

The objective of the study was to evaluate the effects of pattern raising systems on production performance and egg quality of laying hens. One hundred twenty laying hens and 30 weeks of age were used in completely randomized design (CRD). The study was divided into 2 patterns: 1) conventional battery cage pattern; and 2) floor pen pattern. The conventional battery cage pattern had dimension of 30 × 40 × 37 cm with 2 laying hens each while in floor pen pattern was 5 m 2 with 10 laying hens each. Each treatment had 10 replicates (120 laying hens). All laying hens were provided with the same diet during 30 days of experimental period. The results showed that the laying hen pattern systems affected to egg production performance and egg quality. Feed intake and egg production of laying hens in the conventional battery cage pattern were significantly higher than those in the floor pen pattern ( p <0.05). However, hens in floor pens showed better results of feed conversion ratio, egg weight and Haugh unit than those from conventional pattern ( p <0.05).
CONSEQUENCE OF ACCUMULATION INTRATHECAL DEXMEDETOMIDINE BY MEANS OF THE ADJUVANT TO HYPERBARIC BUPIVACAINE FOR ELECTIVE CESAREAN SEGMENT

: Background & Aims: Cesarean segment achieved underneath subarachnoid lump remains frequently attended through visceral aching. Henceforth, numerous adjuvants were strained to discourse the current problematic also to offer protracted postoperatively analgesia. Extremely discerning α2-agonist dexmedetomidine stays progressively exercised by way of an intrathecal adjuvant. Settings and Design: The potential, randomized, binary blinded, measured research. Methodology: LSCS under subarachnoid detuning at Services Hospital Lahore Pakistan from September 2018 to March 2019. Overall seventy cases were registered for our research study. Lump features, hemodynamic limitations, restfulness scores also newborn APGAR notches stayed noted. Information found remained amassed also examined by suitable trials. The p-value of < 0.06 remained measured substantial. Results: Beginning of sensory also motor lump remained expressively quicker in Set D (48 also 49 seconds) associated to Set C (70 also 69 seconds). Period for 2 section sensory reversion, period of sensual also motor lump remained expressively protracted in Set D associated to Set C (145 against 46, 365 against 130 in addition 345 vs 116 minutes). Period for primary analgesic application remained pointedly extended in Set D associated to Set C (425 also 70 minutes). Here remained not any substantial alteration in hemodynamic limitations, also newborn APGAR slashes among sets. Conclusions: The adding of 6 μg dexmedetomidine by way of the intrathecal adjuvant to bupivacaine for cesarean piece rushes also extends carnal in addition motor lump also offers improved perioperatively analgesia deprived of substantial parental also newborn opposing properties.
Esterification and in vitro test of derivatives eugenol from clove (syzygies aromatic) as growth inhibitor of human colorectal cancer cell line HCT-116

Colorectal cancer occupies the third position in the world (more than 940,000 cases). Natural compounds are widely used as anti-cancer such as salicylic acid and eugenol isolated from clove (Syzygium aromaticum). The study of the qualitative relationship of eugenol-derived activity structure in the benzene position associated with modification shows that the compound is active as an invitro cancer therapy compound. This research is carried out chemical synthesis by condensation between eugenol and salicylic acid that produce new compound and conducted invitro biological activity test on colorectal cancer cell HCT-116. This research aims to obtaine a new compound derived eugenol that can inhibit the colorectal cancer cell HCT-116. The result of synthesis showed that condensation between eugenol isolates with salicylic acid (ESA) have yield 75% of white needle crystal with melting point 60-65oC. The result of in-vivo test showed that compound ESA have IC50 of 20.98 ± 2.1 μg / ml and the gossypol standard of natural compounds have IC50 14.22 ± 1.1 μg / ml. The results of this test indicate that the results of this new compound need to be developed further so have a high potential as a colorectal anticancer compound.