Miracle-dz/UniFAR-FTUs huggingface dataset:
mir-106b-5p inhibits the invasion and metastasis of colorectal cancer by targeting cTsa

Background: Although miR-106b-5p has been reported to play a pivotal role in various human malignancies, its role in colorectal cancer (CRC) remains unknown. In this study, we comprehensively investigated miR-106b-5p expression and biologic functions in CRC and the molecular mechanism involved. Patients and methods: miR-106b-5p expression was detected in CRC tissues and cell lines by quantitative reverse transcription-polymerase chain reaction. The effects of miR-106b-5p on metastasis were determined in vitro using transwell assays, and in vivo effects were evaluated using a mouse tail vein injection model. Downstream targets of miR-106b-5p were confirmed using bioinformatics programs, luciferase assays, and rescue experiments. Target gene expression and clinicopathologic parameters were also analyzed. Results: miR-106b-5p expression was lower in CRC tissues than in corresponding nontumorous tissues ( P = 0.009), and miR-106b-5p downregulation was negatively associated with lymph node metastasis ( P = 0.006). Functional assays demonstrated that miR-106b-5p overexpression suppressed CRC cell migration and invasion in vitro and lung metastasis formation in vivo. In addition, luciferase assays confirmed that miR-106b-5p directly bound to the 3 ′ untranslated region of cathepsin A (CTSA) and that miR-106b-5p suppressed CRC cell migration and invasion by targeting CTSA. Clinicopathologic analysis showed that CTSA was significantly upregulated in CRC, and increased CTSA was negatively associated with lymph node metastasis ( P = 0.012). Conclusion: Our findings revealed that miR-106b-5p inhibits CRC metastasis by upregulating CTSA expression, which may lead to novel therapeutic strategies for CRC patients. roles in the regulation of target genes by complementary pairing with the 3 ′ untranslated regions (3 ′ UTRs) of mRNAs, leading to translational repression or mRNA degradation. 3 Evidence has shown that miRNAs are abnormally expressed in various cancers, and dysregulated miRNAs are associated with tumor initiation, promotion, and progression.
Reverse Engineering Large Gene Regulatory Networks

—The regulation of gene expression is a fundamental method by which cells control their behaviour in response to their environment. Constructing dynamic, quantitative mathematical models of gene regulatory networks from expression levels is necessary to predict the actions of cells and to gain valuable insights on the underlying processes. In the past decade, Evolutionary Algorithms (EA) have shown success in ascertaining the parameters of these models. Methods that infer quantitative models of regulatory networks, however, such as EAs, have largely been restricted to very small systems: as the number of genes grows, so does the number of networks that can be perfectly consistent with the gene expression data and the search space containing the one ”biologically correct” set of parameters. Here, I propose to develop a method of inferring detailed, predictive models of gene regulatory networks for larger systems using evolutionary algorithms. The focus will be on exploiting topological properties shared by many regulatory networks to efﬁciently navigate the search space and ﬁnd biologically plausible solutions
18 F-fludrodeoxyglucose maximal standardized uptake value and metabolic tumor burden are associated with major chemotherapy-related tumor markers in nsclc patients

Objective: Metabolic activity and tumor burden are significant for prognosis and metastasis of non-small cell lung cancer (NSCLC), including maximum standardized uptake value (SUV max ), metabolic tumor volume (MTV), and total lesion glycolysis (TLG). Chemotherapy resistance is a great challenge for treating NSCLC patients and is also closely related with several biomarkers such as epidermal growth factor receptor (EGFR), p53, and excision repair cross-complementing group 1 protein (ERCC1). Our purpose was to determine the correlation between positron emission tomography/computed tomography (PET/CT) parameters and tumor markers-related chemotherapy resistance in NSCLC. Methods: Forty-six NSCLC chemotherapy-naïve patients were enrolled. The SUV max , MTV, and TLG were calculated by PET/CT imaging, and expression of EGFR, p53, and ERCC1 were analyzed by immunohistochemistry on tissues. SUV max , MTV, and TLG compared for their performance in predicting the expression of EGFR, p53, and ERCC1 were illustrated with statistical analysis. Results: SUV max was significantly correlated with p53 expression ( P = 0.001), and MTV and TLG were significantly associated with ERCC1 ( P = 0.000; P = 0.000). Furthermore, multiple stepwise regression analysis revealed that SUV max was the primary predictor for p53, MTV and TLG was the primary predictor for ERCC1. SUV max had a sensitivity of 91% and specificity of 50% for the detection of p53 positive. The sensitivities of MTV and TLG were 83% and 80%, and specificities were 69% and 75% for the detection of ERCC1 positive, respectively. When we suggested p53 or ERCC1 positive, the cutoff value of SUV max , MTV, and TLG were 7.68, 23.62, and 129.65 cm 3 , respectively. Conclusion: SUV max , MTV, and TLG were closely associated with p53 and ERCC1’ expressions. Therefore, 18 F-fludrodeoxyglucose PET/CT could be a new way of predicting p53 or ERCC1-related chemotherapy effect in NSCLC patients with more convenience. combination of
ACE inhibitors – angiotensin II receptor antagonists: A useful combination therapy for ischemic heart disease

Morbidity and mortality from cardiovascular diseases are still high, even with the use of the best available therapies. There is mounting evidence that excessive renin-angiotensin system activation triggers much of the damaging and progressive nature of cardiovascular and kidney diseases through expression of angiotensin II. Moreover, angiotensin II play a major role in the development of end organ damage through a variety of inflammatory mechanisms. Today, angiotensins-converting enzyme (ACE) inhibitors and angiotensin II receptor antagonists have clearly demonstrated their efficacy in preventing target organ damage and in reducing cardiovascular morbidity and mortality in ischemic heart disease (IHD). Moreover, the development of angiotensin II receptor antagonists has enabled a large gain in tolerability and safety. Several clinical trials have firmly established that these drugs act on the renin–angiotensin system, reducing the incidence of coronary events with monotherapy and combination therapy. In this review we summarize the role mono- and combined therapy of ACE inhibitors and angiotensin II receptor antagonists play in ischemic heart disease. In this respect the review will improve ideas for developing new formulations with combinations of these drugs in the future.
Digital image processing software for diagnosing diabetic retinopathy from fundus photograph

Objective: The aim of this study was to develop automated software for screening and diagnosing diabetic retinopathy (DR) from fundus photograph of patients with diabetes mellitus. Methods: The extraction of clinically significant features to detect pathologies of DR and the severity classification were performed by using MATLAB R2015a with MATLAB Image Processing Toolbox. In addition, the graphic user interface was developed using the MATLAB GUI Toolbox. The accuracy of software was measured by comparing the obtained results to those of the diagnosis by the ophthalmologist. Results: A set of 400 fundus images, containing 21 normal fundus images and 379 DR fundus images (162 non-proliferative DR and 217 proliferative DR), was interpreted by the ophthalmologist as a reference standard. The initial result showed that the sensitivity, specificity and accuracy of this software in detection of DR were 98%, 67% and 96.25%, respectively. How-ever, the accuracy of this software in classifying non-proliferative and proliferative diabetic retinopathy was 66.58%. The average time for processing is 7 seconds for one fundus image. Conclusion: The automated DR screening software was developed by using MATLAB programming and yielded 96.25% accuracy for the detection of DR when compared to that of the diagnosis by the ophthalmologist. It may be a helpful tool for DR screening in the distant rural area where ophthalmologist is not available. include: Optometry; Visual science; Pharmacology and drug therapy in eye diseases; Basic Sciences; Primary and Secondary eye care; Patient and Quality of
Understanding the perceptions and experiences of the deafblind community about digital games

Abstract Purpose Participation and accessibility issues faced by gamers with multi-sensory disabilities are themes yet to be fully understood by accessible technology researchers. In this work, we examine the personal experiences and perceptions of individuals with deafblindness who play games despite their disability, as well as the reasons that lead some of them to stop playing games. Materials and methods We conducted 60 semi-structured interviews with individuals living with deafblindness in five European countries: United Kingdom, Germany, Netherlands, Greece and Sweden. Results Participants stated that reasons for playing games included them being a fun and entertaining hobby, for socialization and meeting others, or for occupying the mind. Reasons for stop playing games included essentially accessibility issues, followed by high cognitive demand, changes in gaming experience due their disability, financial reasons, or because the accessible version of a specific game was not considered as fun as the original one. Conclusions We identified that a considerable number of individuals with deafblindness enjoy playing casual mobile games such as Wordfeud and Sudoku as a pastime activity. Despite challenging accessibility issues, games provide meaningful social interactions to players with deafblindness. Finally, we introduce a set of user-driven recommendations for making digital games more accessible to players with a diverse combination of sensory abilities. IMPLICATIONS FOR REHABILITATION Digital games were considered a fun and entertaining hobby by participants with deafblindness. Furthermore, participants play games for socialization and meeting others, or for occupying the mind. Digital games provide meaningful social interactions and past time to persons with deafblindness. On top of accessibility implications, our findings draw attention to the importance of the social element of gaming for persons with deafblindness. Based on interviews, we introduce a set of user-driven recommendations for making digital games more accessible to players with a diverse combination of sensory abilities.
Review of lattice results concerning low energy particle physics

We review lattice results related to pion, kaon, D- and B-meson physics with the aim of making them easily accessible to the particle physics community. More specifically, we report on the determination of the light-quark masses, the form factor f+(0), arising in semileptonic K ->pi transition at zero momentum transfer, as well as the decay constant ratio fK/fpi of decay constants and its consequences for the CKM matrix elements Vus and Vud. Furthermore, we describe the results obtained on the lattice for some of the low-energy constants of SU(2)LxSU(2)R and SU(3)LxSU(3)R Chiral Perturbation Theory and review the determination of the BK parameter of neutral kaon mixing. The inclusion of heavy-quark quantities significantly expands the FLAG scope with respect to the previous review. Therefore, for this review, we focus on D- and B-meson decay constants, form factors, and mixing parameters, since these are most relevant for the determination of CKM matrix elements and the global CKM unitarity-triangle fit. In addition we review the status of lattice determinations of the strong coupling constant alpha_s.
Musa sapientum with exercises attenuates hyperglycemia and pancreatic islet cells degeneration in alloxan-diabetic rats

Aim: We tested the hypothesis that administrations of methanolic extracts of Musa sapientum sucker (MEMS) with exercises attenuated hyperglycemia in alloxan-diabetic rats. Materials and Methods: A total of 40 adult male rats were divided into equal eight groups. Normoglycemic Group A was Control. Alloxan (180 mg/kg, i.p.) was administered to rats in Groups B - H to induce diabetes. Group B (diabetic control) received physiological saline. Groups C - H received MEMS (5 mg/kg), MEMS (10 mg/kg), Glibenclamide (5 mg/kg), MEMS (5 mg/kg) + exercises, MEMS (10 mg/kg) + exercises and Exercises only, respectively. Changes in body weight, blood glucose levels (BGL) and pancreatic histology were evaluated during or at the end of experiment. Body weights and BGL of rats were expressed as mean ± standard deviation and analyzed using the statistical software program SPSS 15. Statistical comparisons were done using the Student’s t-test for unpaired samples. Differences between groups were determined as significant at P ≤ 0.05. Results: Significantly (P < 0.05) decreased bodyweight was observed in B and H compared to A and C - G. Treatment with MEMS significantly (P < 0.05) decreased elevated BGL in C and D. Hypoglycemic effect of MEMS appeared enhanced with exercises in F and G. Exercises regimen alone (H) resulted in percentage reduction in BGL lower than those of C - G. Histopathological examinations revealed normal pancreas (A), atrophied islet cells (B), hyperplasia with adequate population of islet cells (C - G), and reduced hyperplasia of islet cells (H). Conclusion: MEMS with exercises attenuated hyperglycemia in alloxan-diabetic rats.
A Multilevel Analysis of Intercompany Claim Counts

Abstract It is common for professional associations and regulators to combine the claims experience of several insurers into a database known as an “intercompany” experience data set. In this paper, we analyze data on claim counts provided by the General Insurance Association of Singapore, an organization consisting of most of the general insurers in Singapore. Our data comes from the financial records of automobile insurance policies followed over a period of nine years. Because the source contains a pooled experience of several insurers, we are able to study company effects on claim behavior, an area that has not been systematically addressed in either the insurance or the actuarial literatures. We analyze this intercompany experience using multilevel models. The multilevel nature of the data is due to: a vehicle is observed over a period of years and is insured by an insurance company under a “fleet” policy. Fleet policies are umbrella-type policies issued to customers whose insurance covers more than a single vehicle. We investigate vehicle, fleet and company effects using various count distribution models (Poisson, negative binomial, zero-inflated and hurdle Poisson). The performance of these various models is compared; we demonstrate how our model can be used to update a priori premiums to a posteriori premiums, a common practice of experience-rated premium calculations. Through this formal model structure, we provide insights into effects that company-specific practice has on claims experience, even after controlling for vehicle and fleet effects.
Cypher: An Evolving � ery Language for Property Graphs

The Cypher property graph query language is an evolving language, originally designed and implemented as part of the Neo4j graph database, and it is currently used by several commercial database products and researchers. We describe Cypher 9, which is the � rst version of the language governed by the openCypher Implementers Group. We � rst introduce the language by example, and describe its uses in industry. We then provide a formal semantic de � nition of the core read-query features of Cypher, including its variant of the property graph data model, and its “ASCII Art” graph pattern matching mechanism for expressing subgraphs of interest to an application. We compare the features of Cypher to other property graph query languages, and describe extensions, at an advanced stage of development, which will form part of Cypher 10, turning the language into a compositional language which supports graph projections and multiple named graphs.
Kalamazoo River Watershed Land Conservation Plan

The Kalamazoo River Watershed Land Conservation Plan was developed to select for conservation targets among ownership parcels in the Kalamazoo River Watershed (MI). The watershed, while historically degraded, features large areas of preserved Midwestern habitats. To facilitate for the permanent protection of these lands, this plan was developed using an ArcGIS-based analysis that scored ownership parcels based on the following conservation criteria: land cover, presence of wetlands, proximity to hydrology, proximity to existing conserved lands, presence of cold lands, and presence of threatened and endangered species habitat. These criteria were developed using a literature review of existing conservation plans and Kalamazoo River Watershed stakeholder input. The results from this analysis were used to identify conservation priorities, including: the top 100 scoring parcels in the basin, a database of the top 20% scoring parcels and their contact information, and priority subwatersheds for conservation.
Towards spontaneous parametric down conversion from monolayer MoSは

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Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2

Type IV collagen is a major component of the basement membrane and interacts with numerous other basement membrane proteins. Many of these interactions are poorly characterized. Type IV collagen is abundantly post-translationally modified with 3-hydroxyproline (3-Hyp), but 3-Hyp's biochemical role in type IV collagen's interactions with other proteins is not well established. In this work, we present binding data consistent with a major role of 3-Hyp in interactions of collagen IV with glycoprotein VI and nidogens 1 and 2. The increased binding interaction between type IV collagen without 3-Hyp and glycoprotein VI has been the subject of some controversy, which we sought to explore, whereas the lack of binding of nidogens to type IV collagen without 3-Hyp is novel. Using tandem MS, we show that the putative glycoprotein VI-binding site is 3-Hyp–modified in WT PFHR-9 type IV collagen, but not in PFHR-9 cells in which prolyl-3-hydroxylase 2 (P3H2) has been knocked out (KO). Moreover, we observed altered 3-Hyp occupancy across many other sites. Using amino acid analysis of type IV collagen from the WT and P3H2 KO cell lines, we confirm that P3H2 is the major, but not the only 3-Hyp–modifying enzyme of type IV collagen. These findings underscore the importance of post-translational modifications of type IV collagen for interactions with other proteins.
Detection of nitroaromatic vapours with diketopyrrolopyrrole thin films : exploring the role of structural

Warzecha, M. , Calvo­Castro, J., Kennedy, A. R. , Macpherson, A. N. , Shankland, K. , Shankland, N. , McLean, A. J. and McHugh, C. J. (2015) Detection of nitroaromatic vapours with diketopyrrolopyrrole thin films: exploring the role of structural order and morphology on thin film properties and fluorescence quenching efficiency. Chemical Communications, 51 (6). pp. 1143­1146. ISSN 1359­7345 doi: https://doi.org/10.1039/c4cc08468c Available at http://centaur.reading. ac.uk/39214/
The Social Costs of Gender Nonconformity for Transgender Adults : Implications for Discrimination and Health 1

Research suggests that transgender people face high levels of discrimination in society, which may contribute to their disproportionate risk for poor health. However, little is known about whether gender nonconformity, as a visible marker of one's stigmatized status as a transgender individual, heightens trans people's experiences with discrimination and, in turn, their health. Using data from the largest survey of transgender adults in the United States, the National Transgender Discrimination Survey (N = 4,115), we examine the associations among gender nonconformity, transphobic discrimination , and health-harming behaviors (i.e., attempted suicide, drug/alcohol abuse, and smoking). The results suggest that gender nonconforming trans people face more discrimination and, in turn, are more likely to engage in health-harming behaviors than trans people who are gender conforming. Our findings highlight the important role of gender nonconformity in the social experiences and well-being of transgender people. INTRODUCTION Gender theorists have long suggested that no social space exists in Western societies for individuals who deviate from binary gender systems (Lorber 1994). Indeed, transgender people are systematically oppressed and experience high rates of discrimination and violence in the United States (Clements et al. 1999; Factor and Rothblum 2008b; Lombardi et al. 2002). For example, trans-gender people frequently face subtle, day-today expressions of discrimination, including others' expressed discomfort, as well as physical threats, and harassment (Nadal, Skolnik, and Wang 2012). However, researchers have not This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The copyright line for this article was changed on 21 September 2015 after original online publication. and the Sociological Forum editor and anonymous reviewers for insightful comments and excellent suggestions on various drafts of this article. Earlier versions of this manuscript were also presented in the Social Interaction and Social Structure Seminar in the Department of Sociology at Indiana University and at the 2012 American Sociological Association annual meeting; we thank members of the seminar and session for their valuable comments and suggestions. 809 examined whether gender nonconformity—as a visible and known marker of one's stigmatized status—shapes trans people's experiences with discrimination and health. This is particularly surprising, given that gender theorists have pos-ited that transgender people primarily face social penalties due to perceptions that they are not normatively " doing gender " (West and Zimmerman …
Crystal structure of pentacarbonyl(2,2-difluoropropanethioato- S )manganese(I)

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Crystal structure of pentacarbonyl(2,2-difluoropropanethioato-κ S )manganese(I) Jean-Claude Daran, Roberto Morales-Cerrada, Christophe Fliedel, Florence Gayet, Vincent Ladmiral, Bruno Ameduri, Rinaldo Poli