Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of novel nutrient-sensitive gene regulatory networks in amniotic fluid from fetuses with spina bifida using miRNA and transcription factor network analysis

Background: Neural tube defects (NTDs) remain among the most common congenital anomalies. Contributing risk factors include genetics and nutrient deficiencies, however, a comprehensive assessment of nutrient-gene interactions in NTDs is lacking. We hypothesised that multiple nutrient-gene interactions would be evident in NTD-associated gene signatures. Methods: We applied a novel, nutrient-focused gene expression analysis pipeline to identify nutrient-sensitive gene regulatory networks in amniocyte gene expression data (GSE4182) from fetuses with NTDs (cases; n=3) and fetuses with no congenital anomalies (controls; n=5). Differentially expressed genes (DEGs) were identified and screened for having nutrient cofactors. Transcription factors (TFs) with nutrient cofactors that regulated DEGs, and nutrient-sensitive miRNAs that had a previous link to NTDs, were identified and used to construct DEG regulatory networks. Results: Of the 880 DEGs in cases (vs. controls), 10% had at least one nutrient cofactor. DEG regulatory network analysis revealed that 39% and 52% of DEGs in cases were regulated by 22 nutrient-sensitive miRNAs and 10 nutrient-dependent TFs, respectively. Zinc- and B vitamin- dependent genes and gene regulatory networks (Zinc: 10 TFs targeting 50.6% of DEGs; B vitamins: 4 TFs [targeting 37.7% of DEGs], 9 miRNAs [targeting 17.6% of DEGs]) were dysregulated in cases. Two nutrient-dependent TFs predicted to target DEGs in cases (Tumor Protein 63 and Churchill Domain Containing 1) have not been previously linked to NTDs. Conclusions: We identified multiple novel nutrient-sensitive gene regulatory networks associated with NTDs, which may relate to NTD pathogenesis, and indicate new targets to explore for NTD prevention or to optimise fetal development.
Mobil eszközök antennáinak méretcsökkentése

A mobil eszkozok meretenek tovabbi csokkentese napjainkban is jelentos kutatasi tema, ugyanis az elofizetoi igenyek folyamatosan ebben az iranyban hatnak. A nagy integraltsagu aramkorok alkalmazasa csak egyik szukseges feltetele a meretcsokkentesnek, a mobil eszkozok meretet ugyanis alapvetoen meghatarozo tovabbi osszetevok a keszulekek miniaturizalasanak jelentos korlatjai. A kijelzo es billentyuzet meretet jelenleg mar nem a technologia, hanem ergonomiai megfontolasok, kenyelmes kezelhetoseg korlatozza. Az akkumulatorok meretenek csokkentese napjainkban is szeleskoruen kutatott terulet, uj anyagok bevezetesevel tovabbi jelentos lepesek varhatoak akar az akkumulatorkapacitas novelesevel egyutt. A cikkben a mobil eszkozok antennainak meretcsokkentesi lehetosegeit es korlatait vizsgaljuk meg, az antennarendszerek teljes meretere, illetve egyedi antennaelemekre reszletesen.
Recommendation Reversals in Gastroenterology Clinical Practice Guidelines

Abstract Background Recommendations in clinical practice guidelines (CPGs) may be reversed when evidence emerges to show they are futile or unsafe. In this study, we identified and characterized recommendation reversals in gastroenterology CPGs. Methods We searched CPGs published by 20 gastroenterology societies from January 1990 to December 2019. We included guidelines which had at least two iterations of the same topic. We defined reversals as when (a) the more recent iteration of a CPG recommends against a specific practice that was previously recommend in an earlier iteration of a CPG from the same body, and (b) the recommendation in the previous iteration of the CPG is not replaced by a new diagnostic or therapeutic recommendation in the more recent iteration of the CPG. The primary outcome was the number of recommendation reversals. Secondary outcomes included the strength of recommendations and quality of evidence cited for reversals. Results Twenty societies published 1022 CPGs from 1990 to 2019. Our sample for analysis included 129 unique CPGs. There were 11 recommendation reversals from 10 guidelines. New evidence was presented for 10 recommendation reversals. Meta-analyses were cited for two reversals, and randomized controlled trials (RCTs) for seven reversals. Recommendations were stronger after the reversal for three cases, weaker in two cases, and of similar strength in three cases. We were unable to compare recommendation strengths for three reversals. Conclusion Recommendation reversals in gastroenterology CPGs are uncommon but highlight low value or harmful practices.
Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines

Drought is one of the most serious abiotic stress factors limiting crop yields. Although sunflower is considered a moderate drought-tolerant plant, drought stress still has a negative impact on sunflower yield as cultivation expands into arid regions. The extent of drought stress is varieties and time-dependent, however, the molecular response mechanisms of drought tolerance in sunflower with different varieties are still unclear. Here, we performed comparative physiological and transcriptome analyses on two sunflower inbred lines with different drought tolerance at the seedling stage. The analysis of nine physiological and biochemical indicators showed that the leaf surface area, leaf relative water content, and cell membrane integrity of drought tolerance inbred line were higher than those of drought-sensitive inbred line under drought stress, indicating that DT had stronger drought resistance. Transcriptome analyses identified 24,234 differentially expressed genes (DEGs). Gene ontology (GO) analysis showed the up-regulated genes were mainly enriched in gibberellin metabolism and rRNA processing, while the down-regulated genes were mainly enriched in cell-wall, photosynthesis, and terpene metabolism. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed genes related to GABAergic synapse, ribosome biogenesis were up-regulated, while genes related with amino sugar and nucleotide sugar metabolism, starch and sucrose metabolism, photosynthesis were down-regulated. Mapman analysis revealed differences in plant hormone-signaling genes over time and between samples. A total of 1,311 unique putative transcription factors (TFs) were identified from all DEGs by iTAK, among which the high abundance of transcription factor families include bHLH, AP2/ERF, MYB, C2H2, etc. Weighted gene co-expression network analysis (WGCNA) revealed a total of 2,251 genes belonging to two modules(blue 4, lightslateblue), respectively, which were significantly associated with six traits. GO and KEGG enrichment analysis of these genes was performed, followed by visualization with Cytoscape software, and the top 20 Hub genes were screened using the CytoHubba plugin.
Dynamic response of chest to blast wave in closed chamber in rats

Objective To explore the dynamic response of rat chest wall to different blast waves and its relationship with the injury severity in closed chamber.Methods Different spherical explosives with different explosive energies equal to 80,200,400 or 600 mg TNT were used to produce blast waves to 4 groups of rats(n=10)respectively in a closed chamber.All rats were anesthetized and with their chest placed 11.5 cm from the detonator.A REDLAKE-HE high-speed camera was used to record the movement of chest wall and the over-pressure of blast wave.The rat injury were analyzed with adjusted severity of injury index(ASⅡ)and light and electron microscopy.Results Chest deformation,and the highest speed of chest wall inward were correlated with ASⅡ(r=0.967 78,P=0.032 22;r=0.968 81,P=0.031 19). Thoracic organs injury due to inward motion of chest wall was viscous damage by blast wave.Conclusion The movement of chest wall appears to be one of the major factors causing the thoracic injuries.The highest speed of chest wall inward is well correlated with ASⅡ,and is a reliable parameter to indicate the shock injury severity.
Perspective: Striking a Balance between Planetary and Human Health—Is There a Path Forward?

ABSTRACT The global adoption of predominantly plant-based, sustainable, healthy diets will help reduce the risk of obesity- and malnutrition-related noncommunicable diseases while protecting the future health of our planet. This review examines the benefits and limitations of different types of plant-based diets in terms of health and nutrition, affordability and accessibility, cultural (ethical and religious) acceptability, and the environment (i.e., the 4 pillars underlying sustainable healthy diets). Results suggest that, without professional supervision, traditional plant-based diets (vegan, vegetarian, and pescatarian diets) can increase the risk of nutritional deficiencies among infants, children/adolescents, women, pregnant/lactating women, and the elderly. In contrast, flexitarian diets and territorial diversified diets (TDDs; e.g., Mediterranean and New Nordic diets) that include large quantities of plant-sourced foods, low amounts of red meat, and moderate amounts of poultry, fish, eggs, and dairy can meet the energy and nutrition needs of different populations without the need for dietary education or supplementation. Compared with vegan, vegetarian, and pescatarian diets, more diverse flexitarian diets and TDDs are associated with reduced volumes of food waste and may be more acceptable and easier to maintain for people who previously followed Western diets. Although flexitarian diets and TDDs have a greater impact on the environment than vegan, vegetarian, and pescatarian diets, the negative effects are considerably reduced compared with Western diets, especially if diets include locally sourced seasonal foods. Further studies are required to define more precisely optimal sustainable healthy diets for different populations and to ensure that diets are affordable and accessible to people in all countries.
The use of a three-dimensional dynamic arm support prevents the development of muscle fatigue during repetitive manual tasks in healthy individuals

Work-related upper extremity disorders are costly to society due to resulting medical costs, presenteeism and absenteeism. Although their aetiology is likely multifactorial, physical workplace factors are known to play an important role in their development. Promising options for preventing work-related upper extremity disorders include assistive technologies such as dynamic arm supports designed to follow the movement of the arm while compensating for its weight. The objective of this study was to assess the effects of a dynamic arm support on perceived exertion, muscle activity and movement patterns of the upper limb during repetitive manual tasks in healthy individuals. Thirty healthy right-handed individuals were allocated either a static or a dynamic task to perform with and without a dynamic arm support. During the task, surface electromyographic activity (anterior and middle deltoid, upper trapezius) and upper limb kinematics (elbow, shoulder, sternoclavicular) were measured using surface EMG and inertial sensors. Results showed that the dynamic arm support significantly reduced perceived exertion during the tasks and limited the development of muscular fatigue of the anterior and middle deltoid as demonstrated by EMG signal mean epoch amplitudes and median frequency of the EMG power spectrum. The dynamic arm support also prevented a decrease in shoulder elevation and an increase in total shoulder joint excursion during static and dynamic task, respectively. These results denote the potential benefits of dynamic arm supports in work environments. Further studies should focus on their efficacy, acceptability and implementability in work settings.
Brazilian Guidelines for Nutrition in Cystic Fibrosis

ABSTRACT Objective To develop a scientific consensus on nutrition in cystic fibrosis. Methods Sixteen coordinators elaborated relevant questions on nutritional therapy in cystic fibrosis, which were divided into six sections: nutritional assessment, nutritional recommendations, nutritional intervention, dietary counseling, special situations and enzyme replacement, and gastrointestinal manifestations. Two to three specialists in the field were responsible for each section and obtaining answers formulated based on standardized bibliographic searches. The available literature was searched in the PubMed®/MEDLINE database, after training and standardization of search strategies, to write the best level of evidence for the questions elaborated. Issues related to disagreement were discussed until a consensus was reached among specialists, based on the current scientific literature. Results Forty-two questions were prepared and objectively answered, resulting in a consensus of nutritional therapy in cystic fibrosis. Conclusion This work enabled establishing a scientific consensus for nutritional treatment of cystic fibrosis patients.
The Study of the Aviation Industrial Technology Convergence through Patent analysis

Nowadays, technologies are changing through industrial fusion and government & corporates need to predict the flow & direction of technologies. These flow & direction can be grasped through the analysis of patent information. The patent information uses the common classification codes in the world, and it is possible for the quantitative analysis based on objective data with the time information of technical area. The methods of patent analysis analyzed the technology fusion by using citation analysis & simultaneous classification analysis. This research analyzed patent information which used as an index to measure the technical innovation in the society based on knowledge, and would like to analyze technical trends and to describe the way of improvement in the future based on the aviation industry which is the representative fusion/complex industry.
Population genomics of the grapevine pathogen Eutypa lata reveals evidence for population expansion and intraspecific differences in secondary metabolite gene clusters

Eutypa dieback of grapevine is an important disease caused by the generalist Ascomycete fungus Eutypa lata. Despite the relevance of this species to the global wine industry, its genomic diversity remains unknown, with only a single publicly available genome assembly. Whole-genome sequencing and comparative genomics was performed on forty Australian E. lata isolates to understand the genome evolution, adaptation, population size and structure of these isolates. Phylogenetic and linkage disequilibrium decay analyses provided evidence of extensive gene flow through sexual recombination between isolates obtained from different geographic locations and hosts. Investigation of the genetic diversity of these isolates suggested rapid population expansion, likely as a consequence of the recent growth of the Australian wine industry. Genomic regions affected by selective sweeps were shown to be enriched for genes associated with secondary metabolite clusters and included genes encoding proteins with a role in nutrient acquisition, degradation of host cell wall and metal and drug resistance, suggesting recent adaptation to both abiotic factors and potentially host genotypes. Genome synteny analysis using long-read genome assemblies showed significant intraspecific genomic plasticity with extensive chromosomal rearrangements impacting the secondary metabolite production potential of this species. Finally, k-mer based GWAS analysis identified a potential locus associated with mycelia recovery in canes of Vitis vinifera that will require further investigations.
The Forensic Psychiatric Network of Observation and Documentation: At the Intersection of Review Board Hearings and Nursing Practice

ABSTRACT Forensic psychiatric nursing is a specialty at the junction of two well-researched intersecting systems with two mandates: criminal justice and health care. Nurses' involvement at one of the systems' points of juncture, review board (RB) hearings, has largely been left unexplored. At RB hearings, a panel of legal and healthcare professionals determines if persons unfit to stand trial (UST) or not criminally responsible on account of mental disorder (NCR) represent significant threats to the safety of the public and orders conditions aimed at keeping the community safe. The aim of this article is to present the results of a critical ethnography that explored how psychiatric and public safety discourses construct the identity of persons UST or NCR during RB hearings as well as nurses' contribution to such identity construction. The main finding is that the forensic psychiatric structure leverages nursing interventions and documentation as evidence of deviancy, so that persons UST or NCR can be objectified and produced as dangerous. Structures sustaining the forensic psychiatric system inscribe nursing care within a disciplinary scheme, rendering the care-and-custody dichotomy insufficient to explain the complex processes at play in forensic psychiatry. These findings have implications for the practice of nurses working in forensic psychiatric settings and for that of other nurses who practice on the medicolegal borderland.
Fabrication and Characterization of Flexible Organic CMOS Integrated Circuits with PMMA dielectric

The authors report flexible complementary inverter and ring oscillation circuits where field-effect transistor elements constructed of pentacene p-channel and fullerene n-type semiconductors and a poly(methyl methacrylate) (PMMA) insulator. By reducing the thickness of the insulating layer, operation voltages were significantly reduced in comparison with those in previous work. The transistor exhibited high electron and hole mobilities of 4.410
Disturbance and management effects on forest soil organic carbon stocks in the Pacific Northwest.

Carbon (C)-informed forest management requires understanding how disturbance and management influence soil organic carbon (SOC) stocks at scales relevant to landowners, forest policy and management professionals. The continued growth of datasets and publications allows powerful synthesis approaches to be applied to such questions at increasingly fine scales. Here, we report results from a synthesis that used meta-analysis of published studies and two large observational databases to quantify disturbance and management impacts on SOC stocks. We conducted this, the third in a series of ecoregional SOC assessments, for the Pacific Northwest, which comprises ~8% of the land area but ~12% of the U.S. forest sector C sink. At the ecoregional level, our analysis indicated that fundamental patterns of vegetation, climate, and topography are far more important controls on SOC stocks than land use history, disturbance or management. However, the same patterns suggested that increased warming, drying, wildland fire, and forest regeneration failure pose significant risks to SOC stocks across the region. Detailed meta-analysis results indicated that wildfires diminished SOC stocks throughout the soil profile, while prescribed fire only influenced surface organic materials and harvesting had no significant overall impact on SOC. Independent observational data corroborated the negative influence of fire on SOC derived from meta-analysis, suggested that harvest impacts may vary sub-regionally with climate or vegetation, and revealed that forests with agricultural uses (e.g., grazing) or legacies (e.g., cultivation) had smaller SOC stocks. We also quantified effects of a range of common forest management practices having either positive (organic amendments, nitrogen (N)-fixing vegetation establishment, inorganic N fertilization) or no overall effects on SOC (other inorganic fertilizers, urea fertilization, competition suppression through herbicides). In order to maximize the management applications of our results, we qualified them with ratings of confidence based on degree of support across approaches. Lastly, similar to earlier published assessments from other ecoregions, we supplemented our quantitative synthesis results with a literature review to arrive at a concise set of tactics for adapting management operations to site-specific criteria.
miR‐126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis‐associated colorectal cancer

Inflammatory bowel disease, characterised by chronic relapsing‐remitting colitis, is a significant risk factor for colorectal cancer (CRC). Previously, we showed that miR‐126 functions as a tumour suppressor in CRC and is inversely correlated with tumour proliferation, metastasis and patient prognosis. In the current study, we documented a protective role for miR‐126 in colitis‐associated CRC (CAC) and its underlying mechanism. We detected downregulated miR‐126 expression during colorectal tumorigenesis in the mouse CAC model and in specimens from patients with CRC. The deficiency of miR‐126 in intestinal epithelial cells (IECs) exacerbated tumorigenesis in mice. We identified CXCL12 as a direct target of miR‐126 in inhibiting the development of colitis and CAC. Moreover, miR‐126 regulated the recruitment of macrophages via CXCL12 and decreased the levels of proinflammatory cytokines (IL‐6, IL‐12 and IL‐23). In addition, IL‐6 secreted by macrophages, which were regulated by cocultured transfected CRC cells, altered the proliferation and migration of colon cells. Our data suggest that miR‐126 exerts an antitumour effect on CAC by regulating the crosstalk between IECs and macrophages via CXCL12‐IL‐6 signalling. Our study contributes to the understanding of cancer progression and suggests miR‐126 as a potential therapy for CRC.
The causes and consequences of pest population variability in agricultural landscapes.

Variability in population densities is key to the ecology of natural systems but also has great implications for agriculture. Farmers' decisions are heavily influenced by their risk-aversion to pest outbreaks that result in major yield losses. However, the need for long-term pest population data across many farms has prevented researchers from exploring the drivers and implications of pest population variability. Here, we demonstrate the critical importance of population variability for sustainable farming by analyzing 13 years of pest densities across >1300 Spanish olive groves and vineyards. Variable populations were more likely to cause major yield losses, but also occasionally created temporal windows when densities fell below insecticide spray thresholds. Importantly, environmental factors regulating pest variability were very distinct from factors regulating mean density, suggesting variability needs to be uniquely managed. Finally, we found diversifying landscapes may be a win-win for conservation and farmers, as diversified landscapes promote less abundant and less variable pest populations. Therefore, we encourage agricultural stakeholders to increase complexity of the landscapes surrounding their farms through conserving/restoring natural habitat and/or diversifying crops.