Miracle-dz/UniFAR-FTUs huggingface dataset:
An analysis of the use of artificial intelligence in education in Spain: The in-service teacher’s perspective

Abstract This study surveyed 445 primary, secondary, and higher education teachers to understand their usage of AI tools in the classroom. Results revealed that while teachers generally have a positive attitude toward AI in education, only 25% of them have actually incorporated AI-based tools into their teaching. Moreover, the most common used tools were ChatGPT, Dall-E, and Midjourney. Finally, primary and secondary school teachers mainly use AI for content creation purposes such as presentations, texts, or videos, without emphasizing student engagement with AI tools. In contrast, higher education teachers utilize AI for academic-technical purposes, explaining AI functioning, obtaining information, and enabling students to experiment with AI tools, as well as research-related tasks like text translation, or data analysis. Based on these results, AI training programs for educators should be tailored to each stage, incorporating a wider variety of AI tools beyond the commonly used ones like ChatGPT.
Classification of Atretic Small Antral Follicles in the Human Ovary

The reproductive lifespan in humans is regulated by a delicate cyclical balance between follicular recruitment and atresia in the ovary. The majority of the small antral follicles present in the ovary are progressively lost through atresia without reaching dominance, but this process remains largely underexplored. In our study, we investigated the characteristics of atretic small antral follicles and proposed a classification system based on molecular changes observed in granulosa cells, theca cells, and extracellular matrix deposition. Our findings revealed that atresia spreads in the follicle with wave-like dynamics, initiating away from the cumulus granulosa cells. We also observed an enrichment of CD68+ macrophages in the antrum during the progression of follicular atresia. This work not only provides criteria for classifying three stages of follicular atresia in small antral follicles in the human ovary but also serves as a foundation for understanding follicular degeneration and ultimately preventing or treating premature ovarian failure. Understanding follicular remodeling in the ovary could provide a means to increase the number of usable follicles and delay the depletion of the follicular reserve, increasing the reproductive lifespan.
Targeting Metabolic Vulnerabilities to Overcome Prostate Cancer Resistance: Dual Therapy with Apalutamide and Complex I Inhibition

Simple Summary Prostate cancer often becomes resistant to drug treatment, causing it to advance aggressively. This study aims to find new ways to treat this challenging cancer. We looked at different types of prostate cancer cells to understand their unique metabolic behaviors. Moreover, we tested a combination of two drugs, Apalutamide (ARN) and mitochondrial complex I inhibitor IACS-010759 (IACS), to see if it could slow down cancer cell growth. This research uncovers how prostate cancer cells use energy differently and suggests a promising approach to treat them by targeting their unique metabolism. This could be a big step forward in improving the treatment of advanced prostate cancer. Abstract Prostate cancer (PCa) often becomes drug-treatment-resistant, posing a significant challenge to effective management. Although initial treatment with androgen deprivation therapy can control advanced PCa, subsequent resistance mechanisms allow tumor cells to continue growing, necessitating alternative approaches. This study delves into the specific metabolic dependencies of different PCa subtypes and explores the potential synergistic effects of combining androgen receptor (AR) inhibition (ARN with mitochondrial complex I inhibition (IACS)). We examined the metabolic behaviors of normal prostate epithelial cells (PNT1A), androgen-sensitive cells (LNCaP and C4-2), and androgen-independent cells (PC-3) when treated with ARN, IACS, or a combination. The results uncovered distinct mitochondrial activities across PCa subtypes, with androgen-dependent cells exhibiting heightened oxidative phosphorylation (OXPHOS). The combination of ARN and IACS significantly curbed cell proliferation in multiple PCa cell lines. Cellular bioenergetics analysis revealed that IACS reduced OXPHOS, while ARN hindered glycolysis in certain PCa cells. Additionally, galactose supplementation disrupted compensatory glycolytic mechanisms induced by metabolic reprogramming. Notably, glucose-deprived conditions heightened the sensitivity of PCa cells to mitochondrial inhibition, especially in the resistant PC-3 cells. Overall, this study illuminates the intricate interplay between AR signaling, metabolic adaptations, and treatment resistance in PCa. The findings offer valuable insights into subtype-specific metabolic profiles and propose a promising strategy to target PCa cells by exploiting their metabolic vulnerabilities.
The interplay between the microbiota, diet and T regulatory cells in the preservation of the gut barrier in inflammatory bowel disease

Inflammatory bowel disease (IBD) is becoming more common in the Western world due to changes in diet-related microbial dysbiosis, genetics and lifestyle. Incidences of gut permeability can predate IBD and continued gut barrier disruptions increase the exposure of bacterial antigens to the immune system thereby perpetuating chronic inflammation. Currently, most of the approved IBD therapies target individual pro-inflammatory cytokines and pathways. However, they fail in approximately 50% of patients due to their inability to overcome the redundant pro inflammatory immune responses. There is increasing interest in the therapeutic potential of T regulatory cells (Tregs) in inflammatory conditions due to their widespread capability to dampen inflammation, promote tolerance of intestinal bacteria, facilitate healing of the mucosal barrier and ability to be engineered for more targeted therapy. Intestinal Treg populations are inherently shaped by dietary molecules and gut microbiota-derived metabolites. Thus, understanding how these molecules influence Treg-mediated preservation of the intestinal barrier will provide insights into immune tolerance-mediated mucosal homeostasis. This review comprehensively explores the interplay between diet, gut microbiota, and immune system in influencing the intestinal barrier function to attenuate the progression of colitis.
Deep Learning-Based Knee MRI Classification for Common Peroneal Nerve Palsy with Foot Drop

Foot drop can have a variety of causes, including the common peroneal nerve (CPN) injuries, and is often difficult to diagnose. We aimed to develop a deep learning-based algorithm that can classify foot drop with CPN injury in patients with knee MRI axial images only. In this retrospective study, we included 945 MR image data from foot drop patients confirmed with CPN injury in electrophysiologic tests (n = 42), and 1341 MR image data with non-traumatic knee pain (n = 107). Data were split into training, validation, and test datasets using a 8:1:1 ratio. We used a convolution neural network-based algorithm (EfficientNet-B5, ResNet152, VGG19) for the classification between the CPN injury group and the others. Performance of each classification algorithm used the area under the receiver operating characteristic curve (AUC). In classifying CPN MR images and non-CPN MR images, EfficientNet-B5 had the highest performance (AUC = 0.946), followed by the ResNet152 and the VGG19 algorithms. On comparison of other performance metrics including precision, recall, accuracy, and F1 score, EfficientNet-B5 had the best performance of the three algorithms. In a saliency map, the EfficientNet-B5 algorithm focused on the nerve area to detect CPN injury. In conclusion, deep learning-based analysis of knee MR images can successfully differentiate CPN injury from other etiologies in patients with foot drop.
A Refined Formula For The Allowable Bearing Pressure In Soils And Rocks Using Shear Wave Velocities

Based on a variety of case histories of site investigations, including extensive bore hole data, laboratory testing and geophysical prospecting at more than 550 construction sites, an empirical formulation is proposed for the rapid determination of allowable bearing pressure of shallow foundations in soils and rocks. The proposed expression is corroborated consistently by the results of the classical theory and its application is proven to be rapid and reliable. Plate load tests have been also carried out at three different sites, in order to further confirm the validity of the proposed method. The latter incorporates only two soil parameters, namely, the in situ measured shear wave velocity and the unit weight. The unit weight may also be determined with sufficient accuracy by means of other proposed empirical expressions, using P or S -wave velocities. It is indicated that once the shear and P -wave velocities are measured in situ by an appropriate geophysical survey, the allowable bearing pressure as well as the coefficient of subgrade reaction and many other elasticity parameters may be determined rapidly and reliably.
Waveform modeling of the November 1987 Superstition Hills earthquakes

Long-period body-wave data recorded at teleseismic distances and strong-motion data at Pasadena for the Superstition Hills earthquakes of 24 November 1987 are modeled to obtain the source parameters. We will refer to the event that occurred at 0153 UT as EQ1 and the event at 1316 UT as EQ2. At all distances the first earthquake appears to be a simple left-lateral strike-slip event on a fault striking NE. It is a relatively deep event with a source depth of 10 km. It has a teleseismic moment of 2.7 × 10^(25) dyne cm. The second and more complex event was modeled in two ways: by using EQ1 as the Green's function and by using a more traditional forward modeling technique to create synthetic seismograms. The first method indicated that EQ2 was a double event with both subevents similar, but not identical to EQ1 and separated by about 7.5 sec. From the synthetic seismogram study we obtained a strike of 305° for the first subevent and 320° for the second. Both have dips of 80° and rakes of 175°. The first subevent has a moment of 3.6 × 10^(25) which is half that of the second. We obtain depths of at least 6 km. The teleseismic data indicate a preferred subevent separation of 30 km with the second almost due south of the first, but the error bounds are substantial. This would suggest that the subevents occurred on conjugate faults. The strong-motion data at PAS, however, imply a much smaller source separation, with the sources probably produced by asperities.
An Update on the Diagnosis and Management of Tic Disorders

Tic disorders (TDs) are a group of common neuropsychiatric disorders of childhood and adolescence. TDs may impact the physical, emotional, and social well-being of the affected person. In this review, we present an update on the clinical manifestations, pathophysiology, diagnosis, and treatment of TDs. We searched the PubMed database for articles on tics and Tourette syndrome. More than 400 articles were reviewed, of which 141 are included in this review. TDs are more prevalent in children than in adults and in males than in females. It may result from a complex interaction between various genetic, environmental, and immunological factors. Dysregulation in the cortico-striato-pallido-thalamo-cortical network is the most plausible pathophysiology resulting in tics. TD is a clinical diagnosis based on clinical features and findings on neurological examination, especially the identification of tic phenomenology. In addition to tics, TD patients may have sensory features, including premonitory urge; enhanced and persistent sensitivity to non-noxious external or internal stimuli; and behavioral manifestations, including attention deficit hyperactivity disorders, obsessive-compulsive disorders, and autism spectrum disorders. Clinical findings of hyperkinetic movements that usually mimic tics have been compared and contrasted with those of TD. Patients with TD may not require specific treatment if tics are not distressing. Psychoeducation and supportive therapy can help reduce tics when combined with medication. Dispelling myths and promoting acceptance are important to improve patient outcomes. Using European, Canadian, and American guidelines, the treatment of TD, including behavioral therapy, medical therapy, and emerging/experimental therapy, has been discussed.
Prolidase deficiency: biochemical study of erythrocyte and skin fibroblast prolidase activity in Italian patients.

BACKGROUND AND METHODS Prolidase deficiency (PD), a rare, autosomally inherited disorder causing iminodipeptiduria is associated with a number of clinical manifestations, the principle feature being chronic skin ulceration. The enzyme prolidase cleaves iminodipeptides containing C-terminal prolyl or hydroxyprolyl residues and is important in the final stages of protein catabolism. We report clinical and biochemical findings in 8 Italian patients with proven prolidase deficiency. There was considerable heterogeneity in age at onset of symptoms (varying from 3-17 years), mental retardation and clinical manifestations (asymptomless to very severe). Prolidase activity was determined in hemolysates of patient erythrocytes and cultured dermal fibroblasts. RESULTS Prolidase activity was found to be deficient, especially against gly-pro. Erythrocyte and fibroblast enzyme was also separated into two forms, a major isoform (I) and a minor one (II) by fast protein liquid chromatography, and activity against different iminodipeptide substrates was tested. Isoform I activity was markedly reduced in all patients as compared to normal controls, while isoform II activity appeared to be unaltered. CONCLUSIONS We were unable to find any correlation between degree of enzyme activity loss and severity of symptoms.
A Novel Nomogram to Predict Symptomatic Intracranial Hemorrhage in Ischemic Stroke Patients After Intravenous Thrombolysis

Objective This study aimed to create and validate a novel nomogram to predict the risk of symptomatic intracranial hemorrhage (sICH) in patients with acute ischemic stroke (AIS) who underwent intravenous thrombolysis (IVT). Methods In this retrospective study, 784 patients with AIS who received IVT were enrolled. The patients were randomly divided into two groups: a training set (n=550, 70%) and a testing set (n=234, 30%). Utilizing multivariable logistic regression analysis, relevant factors for the predictive nomogram were selected. The performance of the nomogram was evaluated using various metrics, including the area under the receiver operating characteristic curve (AUC-ROC), the Hosmer-Lemeshow goodness-of-fit test, calibration plots, and decision curve analysis (DCA). Results Multivariable logistic regression analysis showed that specific factors, including National Institutes of Health Stroke Scale (NIHSS) scores, Early infarct signs (EIS), and serum sodium, were identified as independent predictors of sICH. Subsequently, a nomogram was constructed using these predictors. The AUC-ROC values of the nomogram were 0.864 (95% CI: 0.810–0.919) and 0.831 (95% CI: 0.770–0.891) in the training and the validation sets, respectively. Both the calibration plots and the Hosmer-Lemeshow goodness-of-fit test showed favorable agreement in both the training and the validation sets. Additionally, the DCA indicated the practical clinical utility of the nomogram. Conclusion The novel nomogram, which included NIHSS, EIS and serum sodium as variables, had the potential for predicting the risk of sICH in patients with AIS after IVT.
Piezo1-mediated regulation of smooth muscle cell volume in response to enhanced extracellular matrix rigidity.

BACKGROUND AND PURPOSE Decreased aortic compliance is a precursor to numerous cardiovascular diseases. Compliance is regulated by the rigidity of the aortic wall and the vascular smooth muscle cells (VSMCs). Extracellular matrix stiffening, observed during ageing, reduces compliance. In response to increased rigidity, VSMCs generate enhanced contractile forces that result in VSMC stiffening and a further reduction in compliance. Mechanisms driving VSMC response to matrix rigidity remain poorly defined. EXPERIMENTAL APPROACH Human aortic-VSMCs were seeded onto polyacrylamide hydrogels whose rigidity mimicked either healthy (12 kPa) or aged/diseased (72 kPa) aortae. VSMCs were treated with pharmacological agents prior to agonist stimulation to identify regulators of VSMC volume regulation. KEY RESULTS On pliable matrices, VSMCs contracted and decreased in cell area. Meanwhile, on rigid matrices VSMCs displayed a hypertrophic-like response, increasing in area and volume. Piezo1 activation stimulated increased VSMC volume by promoting calcium ion influx and subsequent activation of PKC and aquaporin-1. Pharmacological blockade of this pathway prevented the enhanced VSMC volume response on rigid matrices whilst maintaining contractility on pliable matrices. Importantly, both piezo1 and aquaporin-1 gene expression were upregulated during VSMC phenotypic modulation in atherosclerosis and after carotid ligation. CONCLUSIONS AND IMPLICATIONS In response to extracellular matrix rigidity, VSMC volume is increased by a piezo1/PKC/aquaporin-1 mediated pathway. Pharmacological targeting of this pathway specifically blocks the matrix rigidity enhanced VSMC volume response, leaving VSMC contractility on healthy mimicking matrices intact. Importantly, upregulation of both piezo1 and aquaporin-1 gene expression is observed in disease relevant VSMC phenotypes.
COMMUNITY BASED ECO-TOURISM IN HULU RENING AS POTENTIAL TOURISM DEVELOPMENT IN BATANG KALI, SELANGOR

The aim of this paper is to study and highlight two elements that can contribute to the development of eco-tourism site which is Hulu Rening, Batang Kali. In order to achieve the goal of this study, it is important to identify the effects of the community’s behaviour and their involvement towards developing eco-tourism. By using qualitative research to emphasize on describing, explaining and understand the discussed topic. Research was conducted through interviews with 30 random selections of the people of Hulu Rening which includes the head of the village while 15 responses were retained for further analysis. The findings of this study identify the effects of social capital and intangible assets within the community of Hulu Rening that contributes towards the development of the area. Their positive values of the people in Hulu Rening can positively help to develop the area into a tourist attraction. However, the study were also able to identify the slight behavioral issues that development planners should not overlook in order to successfully build the area as an eco-tourist location.
The future of coral reefs in the US Virgin Islands: is Acropora palmata more likely to recover than Montastraea annularis complex?

Coral diseases have played a major role in the degradation of coral reefs in the Caribbean, including those in the US Virgin Islands (USVI). In 2005, bleaching affected reefs throughout the Caribbean, and was especially severe on USVI reefs. Some corals began to regain their color as water temperatures cooled, but an outbreak of disease (primarily white plague) led to losses of over 60% of the total live coral cover. Montastraea annularis, the most abundant coral, was disproportionately affected, and decreased in relative abundance. The threatened species Acropora palmata bleached for the first time on record in the USVI but suffered less bleaching and less mortality from disease than M. annularis. Acropora palmata and M. annularis are the two most significant species in the USVI because of their structural role in the architecture of the reefs, the large size of their colonies, and their complex morphology. The future of the USVI reefs depends largely on their fate. Acropora palmata is more likely to recover than M. annularis for many reasons, including its faster growth rate, and its lower vulnerability to bleaching and disease.
Development and pharmacokinetic assessment of a fully canine anti-PD-1 monoclonal antibody for comparative translational research in dogs with spontaneous tumors

ABSTRACT PD-1 checkpoint inhibitors have revolutionized the treatment of patients with different cancer histologies including melanoma, renal cell carcinoma, and non-small cell lung carcinoma. However, only a subset of patients show a dramatic clinical response to treatment. Despite intense biomarker discovery efforts, no single robust, prognostic correlation has emerged as a valid outcome predictor. Immune competent, pet dogs develop spontaneous tumors that share similar features to human cancers including chromosome aberrations, molecular subtypes, immune signatures, tumor heterogeneity, metastatic behavior, and chemotherapeutic response. As such, they represent a valuable parallel patient population in which to investigate predictive biomarkers of checkpoint inhibition. However, the lack of a validated, non-immunogenic, canine anti-PD-1 antibody for pre-clinical use hinders this comparative approach and prevents potential clinical benefits of PD-1 blockade being realized in the veterinary clinic. To address this, fully canine single-chain variable fragments (scFvs) that bind canine (c)PD-1 were isolated from a comprehensive canine scFv phage display library. Lead candidates were identified that bound with high affinity to cPD-1 and inhibited its interaction with canine PD-L1 (cPD-L1). The lead scFv candidate re-formatted into a fully canine IgGD reversed the inhibitory effects of cPD-1:cPD-L1 interaction on canine chimeric antigen receptor (CAR) T cell function. In vivo administration showed no toxicity and revealed favorable pharmacokinetics for a reasonable dosing schedule. These results pave the way for clinical trials with anti-cPD-1 in canine cancer patients to investigate predictive biomarkers and combination regimens to inform human clinical trials and bring a promising checkpoint inhibitor into the veterinary armamentarium.
RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease

RecBCD enzyme is a multi-functional protein that initiates the major pathway of homologous genetic recombination and DNA double-strand break repair in Escherichia coli. It is also required for high cell viability and aids proper DNA replication. This 330-kDa, three-subunit enzyme is one of the fastest, most processive helicases known and contains a potent nuclease controlled by Chi sites, hotspots of recombination, in DNA. RecBCD undergoes major changes in activity and conformation when, during DNA unwinding, it encounters Chi (5′-GCTGGTGG-3′) and nicks DNA nearby. Here, we discuss the multitude of mutations in each subunit that affect one or another activity of RecBCD and its control by Chi. These mutants have given deep insights into how the multiple activities of this complex enzyme are coordinated and how it acts in living cells. Similar studies could help reveal how other complex enzymes are controlled by inter-subunit interactions and conformational changes. SUMMARY RecBCD enzyme is a multi-functional protein that initiates the major pathway of homologous genetic recombination and DNA double-strand break repair in Escherichia coli. It is also required for high cell viability and aids proper DNA replication. This 330-kDa, three-subunit enzyme is one of the fastest, most processive helicases known and contains a potent nuclease controlled by Chi sites, hotspots of recombination, in DNA. RecBCD undergoes major changes in activity and conformation when, during DNA unwinding, it encounters Chi (5′-GCTGGTGG-3′) and nicks DNA nearby. Here, we discuss the multitude of mutations in each subunit that affect one or another activity of RecBCD and its control by Chi. These mutants have given deep insights into how the multiple activities of this complex enzyme are coordinated and how it acts in living cells. Similar studies could help reveal how other complex enzymes are controlled by inter-subunit interactions and conformational changes.