Miracle-dz/UniFAR-FTUs huggingface dataset:
Sodium Dodecyl Sulfate ( SDS ) Polyacrylamide Gel Electrophoresis

Juxtanuclear birefringent caps (FC) containing 10-nm filaments form during the early stages of baby hamster kidney (BHK-21) cell spreading. FC are isolated from spreading cells after replating by treatment with 0.6 M KC1, 1% Triton X100 (Robin & Haas Co., Philadelphia, Pa.) and DNase I in phosphate-buffered saline. Purified FC are birefringent and retain the pattern of distribution of 10-rim filaments that is seen in situ. Up to 90% of the FC protein is resolved as two polypeptides of ~54 ,000 and 55,000 molecular weight on sodium dodecyl sulfate (SDS) polyacrylamide gels. The protein is immunologically and biochemically distinct from tubulin as determined by indirect immunofluorescence, double immunodiffusion, one-dimensional peptide mapping by limited proteolysis in SDS gels, and amino acid analysis. The BHK-21 FC amino acid composition, however, is very similar to that obtained for 10-nm filament protein derived from other sources including brain and smooth muscle. Partial disassembly of 10-rim filaments has been achieved by treatment of FC with 6 mM sodium-potassium phosphate buffer, pH 7.4. The solubilized components assemble into distinct 10-nm filaments upon the addition of 0.171 M sodium chloride.
is a repository copy of Protocol of economic evaluation and equity impact analysis of mHealth and community groups for prevention and control of diabetes in rural Bangladesh in a three-arm cluster randomised controlled trial

Introduction Type 2 diabetes mellitus (T2DM) is one of the leading causes of death and disability worldwide, generating substantial economic burden for people with diabetes and their families, and to health systems and national economies. Bangladesh has one of the largest numbers of adults with diabetes in the South Asian region. This paper describes the planned economic evaluation of a three-arm cluster randomised control trial of mHealth and community mobilisation interventions to prevent and control T2DM and non-communicable diseases’ risk factors in rural Bangladesh (D-Magic trial). Methods and analysis The economic evaluation will be conducted as a within-trial analysis to evaluate the incremental costs and health outcomes of mHealth and community mobilisation interventions compared with the status quo. The analyses will be conducted from a societal perspective, assessing the economic impact for all parties affected by the interventions, including implementing agencies (programme costs), healthcare providers, and participants and their households. Incremental cost-effectiveness ratios (ICERs) will be calculated in terms of cost per case of intermediate hyperglycaemia and T2DM prevented and cost per case of diabetes prevented among individuals with intermediate hyperglycaemia at baseline and cost per mm Hg reduction in systolic blood pressure. In addition to ICERs, the economic evaluation will be presented as a cost–consequence analysis where the incremental costs and all statistically signiicant outcomes will be listed separately. Robustness of the results will be assessed through sensitivity analyses. In addition, an analysis of equity impact of the interventions will be conducted. study Ethical Review The indings of this study will be disseminated through different means within and the wider policy sphere.
Energy Consumption in Wireless Sensor Network Using Improved Statistical Framework

-report by a different application, sensor networks need locations of events for that is to remain anonymous, the unauthorized observers network traffic by analyzing the origin of such events should be unable to detect source known as the anonymity issue, this problem is an important topic in the security of wireless sensor networks as being proposed are based on the assumptions different adversarial techniques has emerged with a variety of proposed Structure innovation is twofold: first, It introduces the notion of "in distinguish ability gaps" and wireless sensor networks; The model provides a quantitative measure of anonymity second, this binary hypothesis testing with nuisance parameters source for statistical problem to oblivion maps. by doing so, we issue the binary code, sample analysis of real valued points the theory study of anonymous sensor networks to be included to change the coding opens the door.
University of Birmingham Monocytes and macrophages in alpha-1 antitrypsin deficiency

: Alpha-1 antitrypsin deficiency (AATD) is a genetic condition characterised by low circulating levels of alpha-1 antitrypsin (AAT), a serine proteinase inhibitor. The most common deficiency variants are the S and Z mutations, which cause the accumulation of misfolded AAT in hepatocytes resulting in endoplasmic reticular stress and insufficient release of AAT into the circulation (<11μmol/L). This leads to liver disease, as well as an increased risk of emphysema due to unopposed proteolytic activity of neutrophil-derived serine proteinases in the lungs. AATD has been traditionally viewed as an inflammatory disorder caused directly by a proteinase-antiproteinase imbalance in the lung, but increasing evidence suggests that low AAT levels may affect other cellular functions. Recently, AAT polymers have been identified in both monocytes and macrophages from AATD patients and evidence is building that these cells may also play a role in the development of AATD lung disease. Alveolar macrophages are phagocytic cells that are important in the lung immune response but are also implicated in driving inflammation. This review explores the potential implications of monocyte and macrophage involvement in non-liver AAT synthesis and the pathophysiology of AATD lung disease.
PARENT AND CHILD REPORTS OF CHILD’S ALEXITHYMIA

Alexithymia, a subclinical trait involving difficulty describing and identifying emotions, is common in a number of psychiatric conditions, including autism. Alexithymia in children is sometimes measured by parent report and sometimes by child self-report, but it is not yet known how well the two measures are related. We investigated this question in a sample of 6- to 11-year-old neurotypical children and their parents ( N = 30 dyads). Parent and child reports were not correlated, and most parents underestimated their child’s level of alexithymia relative to the child’s self-report. This suggests that when asked to report on the child’s alexithymia, children and parents may not be reporting on the same thing. These provocative findings, however, must be considered preliminary because a post-hoc analysis showed the analyses were underpowered. Using simulations, I show that to test whether the presence or absence of a correlation best fit these data, 300 dyads would be needed.
Systemic Anaphylaxis IL-9 Promotes but Is Not Necessary for

Anaphylaxis represents an extreme form of allergic reaction, consisting of a sensitization phase during which allergen-speciﬁc IgE are produced and an acute effector phase triggered by allergen-induced degranulation of mast cells. We studied the role of IL-9, a Th2 cytokine implicated in asthma, in different models of murine anaphylaxis. Using a passive model of systemic anaphylaxis, in which anti-DNP IgE Abs were administered before challenge with DNP-BSA, we found that IL-9-transgenic mice or wild-type mice treated with IL-9 for 5 days were highly sensitive to fatal anaphylaxis. This effect was reproduced in both anaphylaxis-susceptible and -resistant backgrounds (FVB/N or [FVB/N (cid:1) BALB/c] F 1 mice, respectively) and correlated with increased serum concentrations of mouse mast cell protease-1 level, a protein released upon mast cells degranulation. By contrast, IL-9 did not increase the susceptibility to passive cutaneous anaphylaxis. IL-9 expression also increased the susceptibility to fatal anaphylaxis when mice were sensitized by immunization against OVA before challenge with the same Ag. In this model, serum from sensitized, IL-9-transgenic mice was more potent in transferring susceptibility to OVA challenge into naive mice, indicating that IL-9 also promotes the sensitization stage. Finally, using IL-9R-deﬁcient mice, we found that despite its anaphylaxis-promoting activity, IL-9 is dispensable for development of both passive and active anaphylaxis, at least in the C57BL/6 mouse background. Taken together, the data reported in this study indicate that IL-9 promotes systemic anaphylaxis reactions, acting at both the sensitization and effector stages, but is not absolutely required for this process. The Journal of Immunology, 2005, 175: 335–341.
CAREER: Supporting Undergraduate Mental Health by Building a Culture ofWellness in Engineering

Karin Jensen, Ph.D. is a Teaching Assistant Professor in bioengineering at the University of Illinois Urbana-Champaign. Her research interests include student mental health and wellness, engineering student career pathways, and engagement of engineering faculty in engineering education research. She was awarded a CAREER award from the National Science Foundation for her research on undergraduate mental health in engineering programs. Before joining UIUC she completed a post-doctoral fellowship at Sanofi Oncology in Cambridge, MA. She earned a bachelor’s degree in biological engineering from Cornell University and a Ph.D. in biomedical engineering from the University of Virginia.
Autologous Vaccination in Mice Activation and Enhances Therapeutic Rapamycin Promotes Dendritic Cell Inhibition of Mechanistic Target of

Dendritic cells (DCs) are potent inducers of T cell immunity, and autologous DC vaccination holds promise for the treatment of cancers and chronic infectious diseases. In practice, however, therapeutic vaccines of this type have had mixed success. In this article, we show that brief exposure to inhibitors of mechanistic target of rapamycin (mTOR) in DCs during the period that they are responding to TLR agonists makes them particularly potent activators of naive CD8 + T cells and able to enhance control of B16 melanoma in a therapeutic autologous vaccination model in the mouse. The improved performance of DCs in which mTOR has been inhibited is correlated with an extended life span after activation and prolonged, increased expression of costimulatory molecules. Therapeutic autologous vaccination with DCs treated with TLR agonists plus the mTOR inhibitor rapamycin results in improved generation of Ag-speciﬁc CD8 + T cells in vivo and improved antitumor immunity compared with that observed with DCs treated with TLR agonists alone. These ﬁndings deﬁne mTOR as a molecular target for augmenting DC survival and activation, and document a novel pharmacologic approach for enhancing the efﬁcacy of therapeutic autologous DC vaccination. The Journal of Immunology , 2012, 189: 000–000.
International in Computer Science and Software Engineering Sentiment Analysis Based on Opinion Classification Techniques: A Survey

— In present scenario the advancement of blogging services, social websites and electronic media play important role by measuring user’s or client’s messages (for example customer reviews, comments and opinions). Opinion Analysis is imperative term alluded to gathering data through a source using NLP, computational etymology and content investigation technique. It settles by choice using subjective data extraction and also by opinion examination. Opinion analysis recognizes negative reviews and positive reviews by measuring how positively and negatively an entity (public, organization and product) is involved. Sentiment analysis is the region of study to analyze people’s reviews, states of mind, emotions and feelings from composed languages. In this paper, we have introduced a writing study on sentiment analysis survey and the methodologies adopted for it. We have focused on various opinion classiﬁcation techniques, which may performed on any data set. Now a day’s the feature extraction approach which is used by specialists to deal with sentiment analysis which is utilized by legislator, news groups, manufacturing organizations, movies, products etc.
Expression of Carboxypeptidase X M14 Family Member 2 Accelerates the Progression of Hepatocellular Carcinoma via Regulation of the gp130/JAK2/Stat1 Pathway

Background: Carboxypeptidase X, M14 family member 2 (CPXM2) has been reported to be involved with several human malignancies. However, the impact of CPXM2 on human hepatocellular carcinoma (HCC) tumorigenesis has not been studied. Materials and Methods: Using immunohistochemistry, the detailed CPXM2 expression patterns were examined in HCC cell lines and tissues. Additionally, a hepatic stellate cell line overexpressing CPXM2 and an HCC CPXM2-knockdown cell line were established by lipofection of an expression plasmid or short hairpin RNA, respectively. The transfection ef ﬁ ciencies were con ﬁ rmed by reverse transcription-quantitative PCR, Western blotting and immuno ﬂ uorescence. Moreover, Western blotting was conducted to determine the phosphorylation levels of the tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription 3 (Stat1) pathway. Furthermore, gp130-speci ﬁ c hairpin RNA was used to knockdown gp130 expression in hepatic stellate cells overexpressing CPXM2. The malignant phenotype of cultured HCC cells was assessed by a Cell Counting Kit-8 (CCK8) assay, plate cloning assay, Matrigel invasion assay and wound-healing assay in vitro. Results: It was demonstrated that CPXM2 was upregulated in HCC, and its upregulation predicted a poor prognosis. Besides, the upregulation of CPXM2 markedly enhanced the metastatic potential of HCC via the gp130/JAK2/Stat1 signaling pathway in vitro. Conclusion: In summary, this evidence suggests a positive role for CPXM2 in HCC progression via modulation of the gp130/JAK2/Stat1 signaling pathway in HCC.
The effect of thermal, flammability, and mechanical properties of wood plastic composites made from recycled food-packaging LDPE and eco-friendly phytic acid

Within this work, a novel eco-friendly flame-retardant system for wood plastic composites (WPCs) was developed based on the phytic acid modified wood flour (WF) and the silane-coupling agents treated diatomite, were incorporated into low density polyethylene (LDPE) by a melt-blending process. Via impregnation process, neutralized phytic acid (PA(N)) was absorbed into the wood porous structure to obtain phytic acid modified wood flour (WF-PA(N)), which was expected to improve the retardancy and thermal stability of the WPC composites without deterioration of their mechanical properties. Moreover, polyethylene grafted maleic anhydride (MAPE) could render the phytic acid modified woods to be well dispersion in LDPE matrix, and consequently increasing the thermal resistance properties of the composites with increasing the content of the phytic acid modified woods. The TGA analysis revealed that the char yield of the LDPE/WF-PA(N) composites (22~29%) were higher than that of LDPE/WF (14%), indicating improved thermal stability by the addition of WF-PA(N). The heat distortion temperature (HDT) of the LDPE/WF-PA(N) composites exhibited upward with increasing WF-PA(N) contents. Among the LDPE/WF-PA(N) composites, the one containing 45% of WF-PA(N) exhibits the best tensile and impact strength. In addition, the burning rate analysis demonstrated that LDPE composites containing WF-PA(N) has short burning rates presumably ascribed to the formation of char layer from phosphorus-containing additives WF-PA(N). This is consistent with the results of TGA analysis.
Implementing data based decision making in logistics processes: Case study at Svenska Mässan

Today utilizing data is crucial for businesses and the logistics sector is no exception. By analyzing and utilizing data, organizations can make better and highly informed decisions. Being able to implement a fluent data collection, analysis and decision-making process, an organization can yield significant competitive advantages. The purpose of this research is to analyze the current traffic data collection system implemented at the case company, Svenska Mässan, to evaluate if it is suitable for decision making in relation to exhibition logistics. Further, the purpose is to find development ideas for the system in order for Svenska Mässan to become more data-driven. The method used for this research is a qualitative case study utilizing primary data collected through interviews and secondary data mostly from a data set that is carefully analyzed. This research concludes that the current data collection system should not be utilized for exhibition logistics. The researchers believe that in order for a system to be used, the case company should implement a tracking id that would help them track each vehicle entering and exiting their premises as well as help them to distinguish between different vehicles. Further, it is suggested that the case company should invest in a consolidation center that is solely managed by them, where all cargo would first be handled. This would improve their operations and minimize the current bottlenecks part of their exhibition logistics processes.
International Journal of Advanced Research in Computer Science and Software Engineering

: In thefield of data mining, utility mining emerges as an important topic therefore mining the high utility itemsets from databases refers to finding the itemsets with high profits. Mining high utility itemsets from a transactional database is referred to the discovery of itemsets with high utility. In the past few years a large number of algorithms have been proposed for mining high utility itemsets from a transactional database. The problem with all those algorithm is the production of a large number of high utility itemsets. Hence the mining performance degrades and it is in terms of execution time and space requirement. Therefore algorithms named UP Growth and UP Growth+ are implemented which requires just two scanning and generate less number of high utility itemset. Performance is further enhanced by implementing the algorithms in Hadoop, so that system with less memory or data set which requires more memory can be analyzed in low memory based system with the help of distributed file system. Overall the performance is upgraded with respect to execution time as well as space. The performance of UP-Growth and UP-Growth+ is compared with the progressive methods on many varieties of each real and artificial information sets. In experimental studies we are going to compare the performances of existing algorithms UP-Growth and UP-Growth+ against the improve UP-Growth and UP-Growth+ victimization dimension reduction with Hadoop.
A REVIEW: SPARSE REPRESENTATION USING MULTIPLE KERNEL APPROACH FOR EFFICIENT AND ACCURATE FACE RECOGNITION

- This review paper highlights accuracy of MKL-SRC(Multiple Kernel Learning Sparse Representation Classification) algorithm on different image classification databases like AR , Extended Yale B, RGBD. Different classification techniques are reviewed. The main objective of MKL-SRC is to classify images in environment having occlusion and noise. MKLSRC using non linear kernel with kernel trick where L1 minimization is used to find sparse representation.
Road Based Travel Recommendation by Mining Important Tourist Location and Travel Sequences Using Geotagged Images

: The problem of recommendation in tourismis to provide important landmark for tourists. This proposed work predicts the user favorite location in a city.Before traveling to particular city the tourist needs information about landmark worth to visit and its order. Without this knowledge the tourist depends on the travel blogs, travel guides and public transportation sites which is time consuming process. In this paper it provides the landmark with user preference categories like education, entertainment etc. Under each categorythe important landmarks are found out. Flickr is a photo sharing website which has launched its service for adding latitude and longitude information to photos called geotagged photos. This information is extracted from flickr using photoid. Based on these geotagged photos information the users preference category get recommended and then ranked the tourist spot. To recommend the travel for the users, the traveling path is also necessary. So to recommend the path, the routing calculation is done. Then the interested travel routing between the landmarks is recommended to the users.
Leader-follower game theory approach for adversarial agents with sensing limitations

Game theory approaches, including those of the Stackelberg form, provide leader-follower strategies for agent patrolling. Specifically we use the developed approaches to agent patrolling in arbitrary spatial environments. The environment is discretized and has a topology structure of a directed graph. The patrolling agent follows a randomized patrol path along the graph. The adversarial agent desires to access certain target nodes in the graph and is assumed to take a certain amount of time to complete the intrusion of a target node. An optimization formulation with the structure constraints is used to provide a patroller strategy that maximizes its expected utility. Several issues arise in providing a game theory solution for an environment that affects sensor performance. Current minimax payoff models for sensing an adversary consider the probability for the defender to sense an adversary. For environmentally limited sensing, this term now has path dependence such as building interiors and areas with transmission issues. However the limitation of sensing was not previously considered and we have modified a constraint to consider this.
Through a different lens: the perceived quality of light field visualization assessed by test participants with imperfect visual acuity and color blindness

Light field visualization technology has progressed significantly in the past two decades. With the emergence of commercially-available devices, both industry and academia have begun research on the potential use cases of future society, including medical visualization, 3D digital signage, telepresence, military applications and many more. During the evaluation and quality assessment of such usage contexts and display types, test participants are typically screened for visual acuity via the Snellen chart and color vision via the Ishihara plates. However, there is an unfortunate global trend that the eyesight of the new generations is getting notably worse, and other sight-related issues, such as color vision deficiency, are becoming more common as well. Therefore, while medical technologies do relentlessly combat the diseases and disorders of the human eye, long-term innovations of visualization must also account for such users. Yet at the time of this paper, those with imperfect vision are underrepresented in light field research. In this paper, we present the results of the series of subjective tests carried out on light field displays, exclusively with test participants that otherwise would not qualify to assess visualization quality in a regular study. The experiments aim at investigating the most relevant research questions of light field visualization quality, such as spatial resolution, angular resolution and viewing distance. Test participants with imperfect visual acuity are classified by the diopters of their corrective lenses, and correlations between diopters and subjective ratings are addressed. Similar analyses were performed for color-blind test participants as well.