Miracle-dz/UniFAR-FTUs huggingface dataset:
Fifteen Years after a 10-year Retrospective: The State of Health Mass Mediated Campaigns

In 2006, a 10-year retrospective of successful mass mediated health communication campaigns reviewed the field and highlighted strategies for successful campaigns. In the 15 years since the retrospective, there have been dramatic changes in the media environment and advances in health communication research. In this article, we describe changes in the health communication media landscape and in our understanding of mass mediated health communication campaigns in the 15 years since the retrospective. Although the media environment has shifted, we argue that effective principles of health communication have remained relatively constant. We note significant advances in technology since the previous retrospective that can further advance health communication campaigns, with new technologies offering promise as channels for health communication campaigns as well as for campaign planning. We also recommend that campaign researchers report in detail on their use of theory, audience segmentation, channel selection, and the role of formative research when describing campaign efforts; that communicators continue to develop frameworks that integrate principles of effective campaign design; and that additional research focus on understanding how technology can be effectively incorporated into campaign planning, distribution, and evaluation.
Inflammatory cytokines induce neutrophil extracellular traps interaction with activated platelets and endothelial cells exacerbate coagulation in moderate and severe essential hypertension

Background: Essential hypertension (EH) patients suffer from paradoxically thrombotic rather than haemorrhagic, although the exact mechanism remains elusive. Our aim is to explore whether and how neutrophil extracellular traps (NETs) play the procoagulant role in EH patients, as well as evaluated whether the NET releasing were triggered by inflammatory cytokines. Methods: The concentration of plasma NETs components were detected by ELISA. The morphology of cells and NETs formation were analysed using immunofluorescence. Procoagulant activity was analysed by clotting time, purified coagulation complex and fibrin generation assays. Phosphatidylserine (PS) exposure on endothelial cells (ECs) was analysed with flow cytometry. Results: Moderate to severe EH patients plasma NETs levels were significantly higher compared to mild EH patients or controls. Furthermore, inflammatory cytokines can induce NETs generation, depleting these patients plasma inflammatory cytokines led to a reduction in NET releasing. NETs from moderate to severe EH patients neutrophils led to significantly decreased clotting time (CT), increased potency to generate thrombin and fibrin (all P < 0.05). These procoagulant effects were markedly attenuated by approximately 70% using DNase I. Additionally, high concentrations NETs exerted a strong cytotoxic effect on ECs, conferring them a procoagulant phenotype. Conclusion: Our study reveals that EH drives a systemic inflammatory environment, which, in turn, drives neutrophils to prime and NET releasing, and found a link between hypercoagulability and NETs levels in moderate to severe EH patients. Therefore, anti-inflammatory combined with block the generation of NETs may represent a new therapeutic target for preventing thrombosis in EH patients.
Efficacy of pembrolizumab in a patient with xeroderma pigmentosum variant and advanced cutaneous squamous-cell carcinoma

Xeroderma pigmentosum (XP) is a rare autosomic recessive disorder of DNA repair pathways that confers photosensitivity along with an increased incidence of ultraviolet (UV)induced cutaneous cancer [1–3]. Eight XP complementation groups (XP-A to XP-G) and an XP variant (XP-V) related to mutations in different genes involved in DNA repair of UVinduced DNA lesions have been described. We described here a patient with a locally advanced and metastatic cutaneous squamous cell carcinoma (SCC) revealing an XP-V successfully treated with PD-1 antibody, pembrolizumab.
Diagnostic Evaluation of Depression Screening Tools in Asian New Zealanders

This study aimed to compare the Kessler Psychological Distress Scale (K10) and Short-Form 12-Item Health Survey Mental Component Score (SF-12 MCS) for the effectiveness as screening tools for depression in Asian New Zealanders. A national, representative New Zealand Health Survey (NZHS) data set was used. In total, 1,277 participants were included in the analyses. The sensitivity and specificity values, as well as the areas under the receiver operating characteristics curves (AUROC), for the K10 and the SF-12 MCS scores were examined. The AUROCs for K10 and SF-12 MCS were 0.787 (95% CI [0.736, 0.837]) and 0.725 (95% CI [0.656, 0.793]), respectively. A less than optimal sensitivity and positive predictive value of K10 support the need to reexamine the optimal cut-off point according to the results of the Youden index. Strengthening the K10 predictive accuracy will increase the practical application among Asian populations.
Randomized Controlled Trial of Group Exercise Intervention for Fall Risk Factors Reduction in Nursing Home Residents

Abstract Objective The aim of this study was to assess the effectiveness of a multidimensional exercise intervention on improving fall risk deterrent factors, such as overall strength and flexibility in nursing home residents. Methods A multi-centre, randomized controlled trial was finally utilized in 40 older adults (>65 years) who were randomly allocated to the intervention or the control group (20 subjects in each). The intervention group attended an exercise program twice a week for eight weeks, to improve functional mobility. The control group did not receive any intervention. Measurements before and after intervention included the Hand Grip Strength (HGS) testing, the Sit-to-Stand test (SST), the Back Scratch Test (BST), and the Sit-and-Reach test (SRT). Results MANOVA revealed significant time effects, V = 0.336, F(6, 33) = 2.78, p = 0.027, partial η2 = 0.336; group effects, V = 0.599, F(6, 33) = 8.22, p < 0.001, partial η2 = 0.599; and group*time interaction, V = 0.908, F(6, 33) = 54.52, p < 0.001, partial η2 = 0.908. A subsequent univariate analysis did not reveal a significant time effect for any variable (p > 0.05). Significant group effects were observed only for SRT (p < 0.05). Significant group*time interactions were observed for all the examined variables (p < 0.05). Dependent t-tests showed that the older adults in the exercise group were significantly improved in all the examined parameters (p < 0.05). Except for SRT (p > 0.05), all the other parameters significantly deteriorated in the control group (p < 0.05). Conclusions Significant improvements were demonstrated in strength and flexibility among nursing home residents following an eight-week group exercise training program.
Single-cell multi-omics of human preimplantation embryos shows susceptibility to glucocorticoids

The preconceptual, intrauterine, and early life environments can have a profound and long-lasting impact on the developmental trajectories and health outcomes of the offspring. Given the relatively low success rates of assisted reproductive technologies (ART; ∼25%), additives and adjuvants, such as glucocorticoids, are used to improve the success rate. Considering the dynamic developmental events that occur during this window, these exposures may alter blastocyst formation at a molecular level, and as such, affect not only the viability of the embryo and the ability of the blastocyst to implant, but also the developmental trajectory of the first three cell lineages, ultimately influencing the physiology of the embryo. In this study, we present a comprehensive single-cell transcriptome, methylome, and small RNA atlas in the day 7 human embryo. We show that, despite no change in morphology and developmental features, preimplantation glucocorticoid exposure reprograms the molecular profile of the trophectoderm (TE) lineage, and these changes are associated with an altered metabolic and inflammatory response. Our data also suggest that glucocorticoids can precociously mature the TE sublineages, supported by the presence of extravillous trophoblast markers in the polar sublineage and presence of X Chromosome dosage compensation. Further, we have elucidated that epigenetic regulation—DNA methylation and microRNAs (miRNAs)—likely underlies the transcriptional changes observed. This study suggests that exposures to exogenous compounds during preimplantation may unintentionally reprogram the human embryo, possibly leading to suboptimal development and longer-term health outcomes.
DAMPS and complement activation in platelet concentrates that induce adverse reactions in patients

Patients with severe thrombocytopenia due to bone marrow failure and after chemotherapy are still treated with platelet transfusions. Platelet concentrates (PC) are associated with a high incidence of adverse reactions (AR). Platelet‐derived damage‐associated molecular patterns (DAMPS) and complement were proposed to play a role in the pathology of AR.
Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics

Abstract Ionizable LNPs are the latest trend in nucleic acid delivery. Microfluidics technology has recently gained interest owing to its rapid mixing, production of nucleic acid-ionizable LNPs, and stability of nucleic acid inside the body. Industrial scale-up, nucleic acid-lipid long-term storage instability, and high production costs prompted scientists to seek alternate solutions to replace microfluidic technology. We proposed a single-pot, organic solvent-free thermocycling technology to efficiently and economically overcome most of the limitations of microfluidic technology. New thermocycling technology needs optimization of process parameters such as sonication duration, cooling–heating cycle, number of thermal cycles, and lipid:aqueous phase ratio to formulate precisely sized particles, effective nucleic acid encapsulation, and better shelf-life stability. Our research led to the formulation of siRNA-ionizable LNPs with particle sizes of 104.2 ± 34.7 nm and PDI 0.111 ± 0.109, with 83.3 ± 4.1% siRNA encapsulation. Thermocycling siRNA-ionizable LNPs had comparable morphological structures with commercialized microfluidics ionizable LNPs imaged by TEM and cryo-TEM. When compared to microfluidics ionizable LNPs, thermocycling siRNA-ionizable LNPs had a longer shelf life at 4°C. Our thermocycling technology showed an effective alternative to microfluidics technology in the production of nucleic acid–ionizable LNPs to meet global demand. Highlights Thermocycling technology is a low-energy, low-temperature, self-assembling cooling–heating process in which lipid droplets spontaneously break apart into much smaller droplets to form siRNA-ionizable LNPs. The new technology is an alternative to multistep, costly, and complex microfluidics technology for the formulation and bulk up of siRNA-ionizable LNPs economically. Thermocycling siRNA-ionizable LNPs formulation focused on optimizing process parameters such as thermal cycle rate, number of thermal cycles, and lipid:aqueous phase ratio. The thermocycling technology is able to overcome the limitations of the storage stability limitations of commercialized ionizable LNPs.
Estimation of the post‐mortem interval by modelling the changes in oral bacterial diversity during decomposition

Decomposition, a complicated process, depends on several factors, including carrion insects, bacteria and the environment. However, the composition of and variation in oral bacteria over long periods of decomposition remain unclear. The current study aims to illustrate the composition of oral bacteria and construct an informative model for estimating the post‐mortem interval (PMI) during decomposition.
In vitro evaluation of platelet extracellular vesicles (PEVs) for corneal endothelial regeneration

Abstract Corneal endothelial cells (CECs) slowly decrease in number with increasing age, which is a clinical issue as these cells have very limited regenerative ability. Therapeutic platelet biomaterials are increasingly used in regenerative medicine and cell therapy because of their safety, cost-effective manufacture, and global availability from collected platelet concentrates (PCs). Platelet extracellular vesicles (PEVs) are a complex mixture of potent bioactive vesicles rich in molecules believed to be instrumental in tissue repair and regeneration. In this study we investigated the feasibility of using a PEVs preparation as an innovative regenerative biotherapy for corneal endothelial dysfunction. The PEVs were isolated from clinical-grade human PC supernatants by 20,000 × g ultracentrifugation and resuspension. PEVs exhibited a regular, fairly rounded shape, with an average size of <200 nm and were present at a concentration of approximately 1011 /mL. PEVs expressed cluster of differentiation 41 (CD41) and CD61, characteristic platelets membrane markers, and CD9 and CD63. ELISA and LC-MS/MS proteomic analyses revealed that the PEVs contained mixtures of growth factors and multiple other trophic factors, as well as proteins related to extracellular exosomes with functional activities associated with cell cadherin and adherens pathways. CECs treated with PEVs showed increased viability, an enhanced wound-healing rate, stronger proliferation markers, and an improved adhesion rate. PEVs did not exert cellular toxicity as evidenced by the maintenance of cellular morphology and preservation of corneal endothelial proteins. These findings clearly support further investigations of PEV biomaterials in animal models for translation as a new CEC regeneration biotherapy.
mtDNA Maintenance and Alterations in the Pathogenesis of Neurodegenerative Diseases

Considerable evidence indicates that the semiautonomous organelles mitochondria play key roles in the progression of many neurodegenerative disorders. Mitochondrial DNA (mtDNA) encodes components of the OXPHOS complex but mutated mtDNA accumulates in cells with aging, which mirrors the increased prevalence of neurodegenerative diseases. This accumulation stems not only from the misreplication of mtDNA and the highly oxidative environment but also from defective mitophagy after fission. In this review, we focus on several pivotal mitochondrial proteins related to mtDNA maintenance (such as ATAD3A and TFAM), mtDNA alterations including mtDNA mutations, mtDNA elimination, and mtDNA release-activated inflammation to understand the crucial role played by mtDNA in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and Huntington's disease. Our work outlines novel therapeutic strategies for targeting mtDNA.
Viral-mediated activation and inhibition of programmed cell death

Viruses are ubiquitous intracellular genetic parasites that heavily rely on the infected cell to complete their replication life cycle. This dependency on the host machinery forces viruses to modulate a variety of cellular processes including cell survival and cell death. Viruses are known to activate and block almost all types of programmed cell death (PCD) known so far. Modulating PCD in infected hosts has a variety of direct and indirect effects on viral pathogenesis and antiviral immunity. The mechanisms leading to apoptosis following virus infection is widely studied, but several modalities of PCD, including necroptosis, pyroptosis, ferroptosis, and paraptosis, are relatively understudied. In this review, we cover the mechanisms by which viruses activate and inhibit PCDs and suggest perspectives on how these affect viral pathogenesis and immunity.
Current strategies with implementation of three-dimensional cell culture: the challenge of quantification

From growing cells in spheroids to arranging them on complex engineered scaffolds, three-dimensional cell culture protocols are rapidly expanding and diversifying. While these systems may often improve the physiological relevance of cell culture models, they come with technical challenges, as many of the analytical methods used to characterize traditional two-dimensional (2D) cells must be modified or replaced to be effective. Here we review the advantages and limitations of quantification methods based either on biochemical measurements or microscopy imaging. We focus on the most basic of parameters that one may want to measure, the number of cells. Precise determination of this number is essential for many analytical techniques where measured quantities are only meaningful when normalized to the number of cells (e.g. cytochrome p450 enzyme activity). Thus, accurate measurement of cell number is often a prerequisite to allowing comparisons across different conditions (culturing conditions or drug and treatment screening) or between cells in different spatial states. We note that this issue is often neglected in the literature with little or no information given regarding how normalization was performed, we highlight the pitfalls and complications of quantification and call for more accurate reporting to improve reproducibility.
Flash: fast, consistent data plane verification for large-scale network settings

Data plane verification can be an important technique to reduce network disruptions, and researchers have recently made significant progress in achieving fast data plane verification. However, as we apply existing data plane verification techniques to large-scale networks, two problems appear due to extremes. First, existing techniques cannot handle too-fast arrivals, which we call update storms, when a large number of data plane updates must be processed in a short time. Second, existing techniques cannot handle well too-slow arrivals, which we call long-tail update arrivals, when the updates from a number of switches take a long time to arrive. This paper presents Flash, a novel system that achieves fast, consistent data plane verification when update arrivals can include update storms, long-tail update arrivals, or both. In particular, Flash introduces a novel technique called fast inverse model transformation to swiftly transform a large block of rule updates to a block of conflict-free updates to efficiently handle update storms. Flash also introduces consistent, efficient, early detection, a systematic mechanism and associated novel algorithms to detect data plane violations with incomplete information, to avoid being delayed by long-tail arrivals. We fully implement Flash and conduct extensive evaluations under various settings. Using the data plane of a large-scale network, we show that compared with state-of-the-art sequential per-update verification systems, Flash is 9,000x faster.
LiveNet: a low-latency video transport network for large-scale live streaming

Low-latency live streaming has imposed stringent latency requirements on video transport networks. In this paper we report on the design and operation of the Alibaba low-latency video transport network, LiveNet. LiveNet builds on a flat CDN overlay with a centralized controller for global optimization. As part of this, we present our design of the global routing computation and path assignment, as well as our fast data transmission architecture with fine-grained control of video frames. The performance results obtained from three years of operation demonstrate the effectiveness of LiveNet in improving CDN performance and QoE metrics. Compared with our prior state-of-the-art hierarchical CDN deployment, LiveNet halves the CDN delay and ensures 98% of views do not experience stalls and that 95% can start playback within 1 second. We further report our experiences of running LiveNet over the last 3 years.
From luna to solar: the evolutions of the compute-to-storage networks in Alibaba cloud

This paper presents the two generations of storage network stacks that reduced the average I/O latency of Alibaba Cloud's EBS service by 72% in the last five years: Luna, a user-space TCP stack that corresponds the latency of network to the speed of SSD; and Solar, a storage-oriented UDP stack that enables both storage and network hardware accelerations. Luna is our first step towards a high-speed compute-to-storage network in the "storage disaggregation" architecture. Besides the tremendous performance gains and CPU savings compared with the legacy kernel TCP stack, more importantly, it teaches us the necessity of offloading both network and storage into hardware and the importance of recovering instantaneously from network failures. Solar provides a highly reliable and performant storage network running on hardware. For avoiding hardware's resource limitations and offloading storage's entire data path, Solar eliminates the superfluous complexity and the overfull states from the traditional architecture of the storage network. The core design of Solar is unifying the concepts of network packet and storage data block - each network packet is a self-contained storage data block. There are three remarkable advantages to doing so. First, it merges the packet processing and storage virtualization pipelines to bypass the CPU and PCIe; Second, since the storage processes data blocks independently, the packets in Solar become independent. Therefore, the storage (in hardware) does not need to maintain receiving buffers for assembling packets into blocks or handling packet reordering. Finally, due to the low resource requirement and the resilience to packet reordering, Solar inherently supports large-scale multi-path transport for fast failure recovery. Facing the future, Solar demonstrates that we can formalize the storage virtualization procedure into a P4-compatible packet processing pipeline. Hence, SOLAR's design perfectly applies to commodity DPUs (data processing units).
Mapping Multimodel Scrolling Techniques for Foot-based Interaction Depending on the Cursor Position

Foot-based input provides an alternative interaction mechanism in the situation where the hands are occupied or unavailable. Our research has implemented and evaluated 5 different mathematical models, i.e., absolute value function, quadratic function, whitey’s square-root function, full continuous function, and modified square root function along with Kobayashi and Igarashi [10] equation for foot-based scrolling techniques. We have considered mapping strategies, mapping techniques, input parameters, and gestural mapping in our design. The proposed techniques were evaluated by 24 participants to analyze the efficiency and accuracy of foot-based scrolling tasks to interact with 2D radiological images. The experimental result shows that there were no significant differences among mapping techniques. However, the users preferred whitney’s square root mapping technique in respect of precision, faster and low fatigues in comparison to other proposed scrolling techniques.
An Overview of Neuromarketing Research in Developing Countries: Prospects and Challenges

Neuromarketing has opened a new door in marketing research understanding behavioral economics with the help of Neuroscience. Over the past decade, Neuroscientists, Psychiatrists, Engineers, and Market-researchers have conducted several groundbreaking studies aiming to understand consumers’ motivations, preferences, and decisions. However, these studies and practices are mainly based on developed countries. In this study, we outline the opportunities, real-life applications, future scenarios and shed light on the challenges faced by the researchers, marketers, and policymakers in developing countries including Bangladesh. Moreover, we have focused on the significant brain lobe involving neuromarketing research with the explanation of current technologies used in this area. We have concluded with some feasible recommendations to continue and sustain the growth of the neuromarketing field in developing countries. We expect that this study will give the directions on the inauguration of neuromarketing research in developing countries like Bangladesh that will help technologists, researchers, and marketers understand the advantages, challenges, and state-of-art of neuromarketing research.