Miracle-dz/UniFAR-FTUs huggingface dataset:
Accurate Chromosome Identification in the Prunus Subgenus Cerasus (Prunus pseudocerasus) and its Relatives by Oligo-FISH

A precise, rapid and straightforward approach to chromosome identification is fundamental for cytogenetics studies. However, the identification of individual chromosomes was not previously possible for Chinese cherry or other Prunus species due to the small size and similar morphology of their chromosomes. To address this issue, we designed a pool of oligonucleotides distributed across specific pseudochromosome regions of Chinese cherry. This oligonucleotide pool was amplified through multiplex PCR with specific internal primers to produce probes that could recognize specific chromosomes. External primers modified with red and green fluorescence tags could produce unique signal barcoding patterns to identify each chromosome concomitantly. The same oligonucleotide pool could also discriminate all chromosomes in other Prunus species. Additionally, the 5S/45S rDNA probes and the oligo pool were applied in two sequential rounds of fluorescence in situ hybridization (FISH) localized to chromosomes and showed different distribution patterns among Prunus species. At the same time, comparative karyotype analysis revealed high conservation among P. pseudocerasus, P. avium, and P. persica. Together, these findings establish this oligonucleotide pool as the most effective tool for chromosome identification and the analysis of genome organization and evolution in the genus Prunus.
Recent Advances in Endocannabinoid System Targeting for Improved Specificity: Strategic Approaches to Targeted Drug Delivery

Opportunities for developing innovative and intelligent drug delivery technologies by targeting the endocannabinoid system are becoming more apparent. This review provides an overview of strategies to develop targeted drug delivery using the endocannabinoid system (ECS). Recent advances in endocannabinoid system targeting showcase enhanced pharmaceutical therapy specificity while minimizing undesirable side effects and overcoming formulation challenges associated with cannabinoids. This review identifies advances in targeted drug delivery technologies that may permit access to the full pharmacotherapeutic potential of the ECS. The design of optimized nanocarriers that target specific tissues can be improved by understanding the nature of the signaling pathways, distribution in the mammalian body, receptor structure, and enzymatic degradation of the ECS. A closer look at ligand-receptor complexes, endocannabinoid tone, tissue distribution, and G-protein activity leads to a better understanding of the potential of the ECS toolkit for therapeutics. The signal transduction pathways examine the modulation of downstream effector proteins, desensitization, signaling cascades, and biased signaling. An in-depth and overall view of the targeted system is achieved through homology modeling where mutagenesis and ligand binding examine the binding site and allow sequence analysis and the formation of libraries for molecular docking and molecular dynamic simulations. Internalization routes exploring receptor-mediated endocytosis and lipid rafts are also considered for explicit signaling. Furthermore, the review highlights nanotechnology and surface modification aspects as a possible future approach for specific targeting.
Construction of Structured Random Measurement Matrices in Semi-Tensor Product Compressed Sensing Based on Combinatorial Designs

A random matrix needs large storage space and is difficult to be implemented in hardware, and a deterministic matrix has large reconstruction error. Aiming at these shortcomings, the objective of this paper is to find an effective method to balance these performances. Combining the advantages of the incidence matrix of combinatorial designs and a random matrix, this paper constructs a structured random matrix by the embedding operation of two seed matrices in which one is the incidence matrix of combinatorial designs, and the other is obtained by Gram–Schmidt orthonormalization of the random matrix. Meanwhile, we provide a new model that applies the structured random matrices to semi-tensor product compressed sensing. Finally, compared with the reconstruction effect of several famous matrices, our matrices are more suitable for the reconstruction of one-dimensional signals and two-dimensional images by experimental methods.
Local Luminosity Function at 15 Microns and Galaxy Evolution Seen by ISOCAM 15 Micron Surveys

A local luminosity function at 15 μm is derived using the bivariate (15 μm vs. 60 μm luminosity) method, based on the newly published ISOCAM LW3 band (15 μm) survey of the very deep IRAS 60 μm sample in the north ecliptic pole region (NEPR). New IRAS 60 μm fluxes are obtained using the SCANPI/SUPERSCANPI software at the new ISOCAM positions of the sources in the sample. It is found to be in excellent agreement with the 15 μm local luminosity function published by Xu et al., which is derived from the predicted 15 μm luminosities of a sample of IRAS 25 μm-selected galaxies. Model predictions of number counts and redshift distributions based on the local luminosity function and assumptions of its evolution with redshift are calculated and compared with the data of ISOCAM 15 μm surveys. Strong luminosity evolution on the order of L ∝ (1 + z)4.5 is suggested in these comparisons, while pure density evolution can be ruled out with high confidence. The sharp peak at about 0.4 mJy in the Euclidean normalized differential counts at 15 μm can be explained by the effects of mid-infrared broadband emission features, eliminating the need for any hypothesis for a "new population. " It is found that the contribution from the population represented by ISOCAM 15 μm sources can account for the entire IR/submillimeter background, leaving little room for any missing "new population" which can be significant energy sources of the IR/submillimeter sky.
Tailoring Morphology and Properties of Tight Utrafiltration Membranes by Two-Dimensional Molybdenum Disulfide for Performance Improvement

To enhance the permeation and separation performance of the polyethersulfone (PES) tight ultrafiltration (TUF) membrane, two-dimensional molybdenum disulfide (MoS2) was applied as a modifier in low concentrations. The influence of different concentrations of MoS2 (0, 0.25, 0.50, 1.00, and 1.50 wt%) on TUF membranes was investigated in terms of morphology, mechanical strength properties, permeation, and separation. The results indicate that the blending of MoS2 tailored the microstructure of the membrane and enhanced the mechanical strength property. Moreover, by embedding an appropriate amount of MoS2 into the membrane, the PES/MoS2 membranes showed improvement in permeation and without the sacrifice of the rejection of bovine serum protein (BSA) and humic acid (HA). Compared with the pristine membrane, the modified membrane embedded with 0.5 wt% MoS2 showed a 36.08% increase in the pure water flux, and >99.6% rejections of BSA and HA. This study reveals that two-dimensional MoS2 can be used as an effective additive to improve the performance and properties of TUF membranes for water treatment.
First Isolation and Identification of Homologous Recombination Events of Porcine Adenovirus from Wild Boar

Porcine adenoviruses (PAdVs) are distributed in pig populations and classified into five immunologically distinct serotypes (PAdV-1 to 5). In this study, a PAdV was isolated from a fecal sample of wild boar for the first time. Whole-genome analysis revealed that this strain (Ino5) has sequence homology (approximately > 93%) throughout the genome with the PAdV-5 strain HNF-70 that was isolated from a pig in Japan in 1987, except for the hexon, E3 612R, and fiber coding regions. Two possible recombination breakpoints were detected in the hexon and E3 612R regions, which were found to have reduced GC content. Structural prediction analysis showed that a part of the hexon protein corresponding to the tower region of Ino5 had structural differences when compared with HNF-70, suggesting antigenic heterogeneity between these strains. PAdVs were detected in 1.77% (2/113) and 12% (12/100) of the fecal samples from wild boars and pigs collected in Japan by PCR, respectively. Phylogenetic analyses of the hexon and fiber genes revealed that some samples showed different grouping in the hexon and fiber genes, suggesting that these viruses have recombination events. These findings suggest that the PAdV-5 has evolved with homologous recombination events in the same manner as human adenoviruses among not only pig populations, but also wild boars in Japan.
The Prevalence of Sarcopenia among Hunan Province Community-Dwelling Adults Aged 60 Years and Older and Its Relationship with Lifestyle: Diagnostic Criteria from the Asian Working Group for Sarcopenia 2019 Update

Background and Objectives: This study aims to detect the prevalence of sarcopenia in community-dwelling older adults in Hunan Province, discuss factors related to lifestyle, and provide a reliable basis for the prevention and treatment of sarcopenia. Materials and Methods: In this study, a total of 1040 community-dwelling adults ≥ 60 years were examined for sarcopenia using a cluster stratified random sampling method, which was defined using the diagnostic criteria recommended by the Asian Working Group for Sarcopenia (AWGS) from September 2019 to March 2020. Multivariate logistic regression analysis was applied to determine the correlation between sarcopenia and smoking, drinking, nutritional status, physical activity, and sleep quality. Results: A total of 27.1% of the older adults were diagnosed with sarcopenia, with rates of 26.2% in men and 25.2% in women. Multiple logistic regression showed that advanced age (OR = 2.480, 95% CI: 1.730, 3.553), the risk of malnutrition (OR = 2.085, 95% CI: 1.440, 3.019), and malnutrition (OR = 1.212, 95% CI: 0.304, 4.834) were risk factors for sarcopenia. No falls in the previous year (OR = 0.616, 95% CI: 1.885, 1.209), normal weight (OR = 0.228, 95% CI: 0.109, 0.475), overweight (OR = 0.030, 95% CI: 0.013, 0.069), moderate physical activity (OR = 0.593, 95% CI: 0.377, 0.933), or high physical activity (OR = 0.417, 95% CI: 0.230, 0.755) were identified as protective factors for sarcopenia. Conclusions: The prevalence of sarcopenia was high among older adults in the community in Hunan Province. In addition, we found that lifestyle is an important factor in sarcopenia.
Galvano-rotational effect induced by electroweak interactions in pulsars

We study electroweakly interacting particles in rotating matter. The existence of the electric current along the axis of the matter rotation is predicted in this system. This new galvano-rotational effect is caused by the parity violating interaction between massless charged particles in the rotating matter. We start with the exact solution of the Dirac equation for a fermion involved in the electroweak interaction in the rotating frame. This equation includes the noninertial effects. Then, using the obtained solution, we derive the induced electric current which turns out to flow along the rotation axis. We study the possibility of the appearance of the galvano-rotational effect in dense matter of compact astrophysical objects. The particular example of neutron and hypothetical quark stars is discussed. It is shown that, using this effect, one can expect the generation of toroidal magnetic fields comparable with poloidal ones in old millisecond pulsars. We also briefly discuss the generation of the magnetic helicity in these stars. Finally we analyze the possibility to apply the galvano-rotational effect for the description of the asymmetric neutrino emission from a neutron star to explain pulsars kicks.
Wet-Spun Polycaprolactone Scaffolds Provide Customizable Anisotropic Viscoelastic Mechanics for Engineered Cardiac Tissues

Myocardial infarction is a leading cause of death worldwide and has severe consequences including irreversible damage to the myocardium, which can lead to heart failure. Cardiac tissue engineering aims to re-engineer the infarcted myocardium using tissues made from human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to regenerate heart muscle and restore contractile function via an implantable epicardial patch. The current limitations of this technology include both biomanufacturing challenges in maintaining tissue integrity during implantation and biological challenges in inducing cell alignment, maturation, and coordinated electromechanical function, which, when overcome, may be able to prevent adverse cardiac remodeling through mechanical support in the injured heart to facilitate regeneration. Polymer scaffolds serve to mechanically reinforce both engineered and host tissues. Here, we introduce a novel biodegradable, customizable scaffold composed of wet-spun polycaprolactone (PCL) microfibers to strengthen engineered tissues and provide an anisotropic mechanical environment to promote engineered tissue formation. We developed a wet-spinning process to produce consistent fibers which are then collected on an automated mandrel that precisely controls the angle of intersection of fibers and their spacing to generate mechanically anisotropic scaffolds. Through optimization of the wet-spinning process, we tuned the fiber diameter to 339 ± 31 µm and 105 ± 9 µm and achieved a high degree of fidelity in the fiber structure within the scaffold (fiber angle within 1.8° of prediction). Through degradation and mechanical testing, we demonstrate the ability to maintain scaffold mechanical integrity as well as tune the mechanical environment of the scaffold through structure (Young’s modulus of 120.8 ± 1.90 MPa for 0° scaffolds, 60.34 ± 11.41 MPa for 30° scaffolds, 73.59 ± 3.167 MPa for 60° scaffolds, and 49.31 ± 6.90 MPa for 90° scaffolds), while observing decreased hysteresis in angled vs. parallel scaffolds. Further, we embedded the fibrous PCL scaffolds in a collagen hydrogel mixed with hiPSC-CMs to form engineered cardiac tissue with high cell survival, tissue compaction, and active contractility of the hiPSC-CMs. Through this work, we develop and optimize a versatile biomanufacturing process to generate customizable PCL fibrous scaffolds which can be readily utilized to guide engineered tissue formation and function.
Interferon–Inducible Transmembrane Protein 3 (IFITM3) Restricts Rotavirus Infection

Rotavirus (RV) is a non–enveloped icosahedral virus with an 11–segment double–stranded RNA genome, belonging to the family of rotaviruses. RV is one of the pathogens causing diarrhea in infants and young animals, and it induces the production of type I interferons (IFNs), which can trigger antiviral function by inducing the production of interferon–stimulated genes (ISGs). Although IFITM3, an ISG localizing to late endosomes, can limit many viral infections, whether or not it restricts the infection of RV is still unknown. Therefore, we attempted to determine whether IFITM3 also restricts RV infection by using over–expression and knockout cell strains. It was found that IFITM3–expressing cell strains were less susceptible to RV infection, as the replication of RV in over–expressing cells was significantly less than in control group cells. Correspondingly, IFITM3–knockout cells were significantly susceptible compared to the normal cells. Furthermore, the IFN–induced antiviral effect was significantly attenuated in the absence of IFITM3, and IFITM3 delayed RV escape from endosomes in the presence of IFITM3, suggesting that endogenous IFITM3 is of great importance in type I IFN–mediated antiviral responses and may restrict infection by affecting the function of the late endosomal compartment. In conclusion, these data provide the first evidence that IFITM3 limits RV infection in vitro and delays RV escape from late endosomes into the cytoplasm.
A Comparative Study of Gamma-Ray Irradiation-Induced Oxidation: Polyethylene, Poly (Vinylidene Fluoride), and Polytetrafluoroethylene

Radiation techniques are used to modify the physical, chemical and biological properties of polymers. This induces crosslinking and degradation reactions of polymers by utilizing radicals generated through ionizing radiation. However, oxidation products (such as carbonyl) can be formed because oxidation occurs by chain scission in the presence of oxygen. Herein, we demonstrate the gamma-ray irradiation-induced oxidation with and without fluorine using polyethylene, polyvinylidene fluoride and polytetrafluoroethylene under the same conditions. In this study, changes in element-content and chemical-bond structures were analyzed before and after gamma-ray irradiation under air atmosphere. As a result, polytetrafluo-roethylene showed less oxidation and excellent thermal properties after the absorbed dose of 500 kGy. This can be attributed to the generation of stable perfluoroalkylperoxy radicals after gamma ray irradiation in the PTFE structure containing only CF2 groups, thereby hindering the oxidation reaction.
Theoretical Analysis of Optical Properties for Amorphous Silicon Solar Cells with Adding Anti-Reflective Coating Photonic Crystals

In the current study, we aim to limit the power dissipation in amorphous silicon solar cells by enhancing the cell absorbance at different incident angles. The current improvement is justified by adding the single-period of ternary 1D photonic crystal with texturing on the top surface, which acts as an anti-reflecting coating. The texturing shape gives the photons at least two chances to localize inside the active area of the cell. Therefore, it increases the absorbance of the cell. Moreover, we add binary one-dimensional photonic crystals with the features of a photonic band gap, which acts as a back mirror to return the photons that were transmitted inside the cell’s active region. The considered structure is demonstrated by the well-defined finite element method (FEM) by using COMSOL multiphysics.
Measured response of a liquid scintillation detector to quasi-monoenergetic electrons and neutrons

The response of a liquid scintillator (EJ-301) to monoenergetic electrons tagged by Compton back scattered γ-rays has been measured using various radioactive γ-ray sources. It is observed that the measured electron response is linear up to an energy of ∼4 MeV. The resolution of the liquid scintillator at an energy 1 MeVee is found to be ∼ 11%. The pulse shape discrimination and pulse height response of the liquid scintillator for neutrons have been measured using the 7Li(p,n1)7Be*(0.429 MeV) reaction. A nonlinear response to mono-energetic neutrons for the liquid scintillator is observed at energies En=5.3 MeV, 9.0 MeV and 12.7 MeV. In addition, the measured response of the liquid scintillator for electrons and neutrons has been compared with simulated results obtained using the Monte Carlo based Geant4 toolkit.
Water and OH Emission from the Inner Disk of a Herbig Ae/Be Star

We report the detection of hot H2O and OH emission from the Herbig Ae/Be star HD 101412 using the Cryogenic Infrared Echelle Spectrograph on the Very Large Telescope. Previous studies of Herbig Ae/Be stars have shown the presence of OH around some of these sources, but H2O has proven more elusive. While marginal water emission has been reported in the mid-infrared, and a few Herbig Ae/Be stars show water emission in the far-infrared, water emission near 2.9 μm has not been previously detected. We apply slab models to the rovibrational OH, H2O, and CO spectra of this source and show that the molecules are consistent with being cospatial. We discuss the possibility that the detection of the CO overtone bandhead emission, detection of water emission, and the large line to continuum contrast of the OH lines may be connected to its high inclination and the λ Boö nature of this star. If the low abundance of refractories results from the selective accretion of gas relative to dust, the inner disk of HD 101412 should be strongly dust-depleted, allowing us to probe deeper columns of molecular gas in the disk, enhancing its molecular emission. Our detection of C- and O-bearing molecules from the inner disk of HD 101412 is consistent with the expected presence, in this scenario, of abundant volatiles in the accreting gas.
Characterization and optimization of chelating resin performance evaluation of ion-exchange using polymeric Dowex-M4195

This research used bispicolamine chelating resin Dowex-M4195 as a polymeric resin material as an alternative for investigating the behavior of synthetic Cu (II) citrate solution adsorbed onto resin surface in a batch experiment system at a low pH of less than 2. Characterization of physical and chemical properties of before and after ion exchange were also explored through BET, SEM-EDX, FTIR and XRD. Response Surface Methodology (RSM) was also applied for optimization of Cu (II) removal capacity using Design of Experiment (DOE) for selective chelating resin. The results indicate that H+ chelating resin Dowex-M4195 presented a high-carbon content and specific surface area of > 64 % and 26.5060 m2 g-1 respectively and have predominantly macropore porous in nature due to the N2 gas adsorption isotherm exhibited characteristics type IV showing the insignificant desorption hythelysis loop of H1-type, spherical and cylindrical. After ion exchange, Cu (II) loaded H+ Dowex-M4195, and the specific surface area and total pore volume decreased of about 17.82 % and 5.39 % as difference respectively. While hythelysis loop, isotherm, and poses size distribution were also similar. Regarding the Functional group, the surface morphology and crystalline structures of H+ Dowex-M4195 showed Cu (II) compound on the structure of chelating resin that confirmed effective ion-exchange behavior. The studies indicate that the H+ Dowex-M4195 is commercially available on the market and can successfully be applied as an alternative precursor for the ion exchange for further reuse and regeneration of the Cu (II) in the E-waste industries.
Changes in C-S-H of Alkali-Activated Slag and Cement Pastes After Accelerated Carbonation

C-S-H gel of waterglassand NaOH-activated slag pastes (AAS) was analysed after accelerated carbonation and compared to the carbonation of C-S-H in Portland cement pastes. TG/DTA, FTIR and Si and Al NMR MAS analyses were used to determine the changes in the C-S-H gel. The different structure and composition of the C-S-H gel in AAS and Portland cement pastes before carbonation is the reason of the different carbonation mechanism in both systems. The carbonation of the C-S-H gel of AAS and Portland conducts to CaCO3, silica gel and a C-S-H gel with a low Ca/Si. However, in AAS pastes, the Al in the silicate chains in the C-S-H gel is removed and a compound with Al tetrahedral is formed, development not observed in Portland cement pastes.