Miracle-dz/UniFAR-FTUs huggingface dataset:
Paradoxical improvement of life quality in the COVID-19 era in psoriasis patients

Background Psoriasis is a chronic immune-mediated inflammatory disease. Beyond the physical dimensions, the disease has an extensive emotional and psychosocial effect on patients, influencing their quality of life, social life and interpersonal relationships. Thus patient-reported outcomes are a crucial instrument for the evaluation of disease burden. Navigating life in times of the COVID-19 pandemic is challenging, especially for persons suffering from chronic diseases. We here analyzed the impact of lockdown restrictions on psoriasis patients. Objective To compare the Dermatology Life Quality Index (DLQI) before and during the COVID-19 pandemic of patients with psoriasis. Methods Retrospective longitudinal analysis in adult patients with moderate to severe psoriasis undergoing biologic treatment between January 2020 and January 2021. DLQI, patient demographics, Psoriasis Area and Severity Index (PASI), and recent biologic treatment were recorded. Results 103 patients were identified, of whom 19 had additional psoriatic arthritis. Female (n = 29) and male (n = 74) patients were distributed 1 to 3. Median age of patients was 54 years (range 18–85). All patients received biologic systemic treatment: anti-IL-23 (n = 39), anti-IL-17A (n = 30), anti-IL-12/23 (n = 25), or anti-TNFα (n = 9). Comparing DLQI scores before the COVID-19 pandemic and under lockdown restriction showed improved DLQI scores over time. Further analysis displayed that patients mostly ticked “not relevant” on social activities during lockdown. Thus, the DLQI scores may be artificial improved and may not really reflect the actual disease burden. Conclusions Psoriasis patients showed a contrary improvement of life quality despite harsh COVID-19 lockdown suggesting that DLQI should be modified when social life is restricted.
DNA sensor associated type I Interferon signalling is increased in ulcerative colitis and induces JAK-dependent inflammatory cell death in colonic organoids.

DNA sensor pathways can initiate inflammasome, cell death and type I interferon (IFN) signalling in immune-mediated inflammatory diseases (IMIDs); including type I interferonopathies. We investigated the involvement of these pathways in the pathogenesis of ulcerative colitis (UC); by analysing expression of DNA sensor, inflammasome, and type I IFN biomarker genes in colonic mucosal biopsy tissue from control (n=31), inactive UC (n=31), active UC (n=33) and a UC single cell RNA-Seq dataset. The effects of type I IFN (IFN-β), IFN-γ and TNF-α on gene expression, cytokine production and cell death were investigated in human colonic organoids. In organoids treated with cytokines alone, or in combination with NLRP3, caspase or JAK inhibitors, cell death was measured, and supernatants were assayed for IL-1β/IL-18/CXCL10. The expression of DNA sensor pathway genes - PYHIN family members (AIM2, IFI16, MNDA, PYHIN1), as well as ZBP1, cGAS and DDX41 were increased in active UC and expressed in a cell type restricted pattern. Inflammasome genes (CASP1, IL1B, IL18), type I IFN inducers (STING, TBK1, IRF3), IFNB1 and type I IFN biomarker genes (OAS2, IFIT2, MX2) were also increased in active UC. Co-treatment of organoids with IFN-β or IFN-γ and TNFα increased expression of IFI16, ZBP1, CASP1, cGAS and STING, induced cell death and IL-1β/IL-18 secretion. This inflammatory cell death was blocked by the JAK inhibitor tofacitinib but not by inflammasome or caspase inhibitors. Increased type I IFN activity may drive elevated expression of DNA sensor genes and JAK-dependent but inflammasome-independent inflammatory cell death of colonic epithelial cells in UC.
Lessons Learned from EXMOS User Studies: A Technical Report Summarizing Key Takeaways from User Studies Conducted to Evaluate The EXMOS Platform

In the realm of interactive machine-learning systems, the provision of explanations serves as a vital aid in the processes of debugging and enhancing prediction models. However, the extent to which various global model-centric and data-centric explanations can effectively assist domain experts in detecting and resolving potential data-related issues for the purpose of model improvement has remained largely unexplored. In this technical report, we summarise the key findings of our two user studies. Our research involved a comprehensive examination of the impact of global explanations rooted in both data-centric and model-centric perspectives within systems designed to support healthcare experts in optimising machine learning models through both automated and manual data configurations. To empirically investigate these dynamics, we conducted two user studies, comprising quantitative analysis involving a sample size of 70 healthcare experts and qualitative assessments involving 30 healthcare experts. These studies were aimed at illuminating the influence of different explanation types on three key dimensions: trust, understandability, and model improvement. Results show that global model-centric explanations alone are insufficient for effectively guiding users during the intricate process of data configuration. In contrast, data-centric explanations exhibited their potential by enhancing the understanding of system changes that occur post-configuration. However, a combination of both showed the highest level of efficacy for fostering trust, improving understandability, and facilitating model enhancement among healthcare experts. We also present essential implications for developing interactive machine-learning systems driven by explanations. These insights can guide the creation of more effective systems that empower domain experts to harness the full potential of machine learning
Oral Contraceptives and Female Rowers' Skeletal Health

Abstract Redinger, AL and Baker, BS. Oral contraceptives and female rowers' skeletal health. J Strength Cond Res 37(3): 669–677, 2023—Previous studies suggest that women using oral contraceptives (OC) experience fewer skeletal benefits from exercise compared with non-OC users. These findings may be especially important for athletes competing in weight-supported sports with a high prevalence of low bone mineral density and fracture, such as rowing. The purpose of this study was to examine skeletal health and bone injuries in collegiate female rowers. Forty-nine National Collegiate Athletic Association Division I female rowers completed general health, menstrual history, and bone physical activity questionnaire (BPAQ) surveys. Dual-energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT) scans were used to assess bone content, density, and geometry. Contraceptive (OC users n = 14, non-OC users n = 35) and self-reported stress fracture (SFx n = 11, None n = 38) groups were analyzed using analysis of covariance and independent t-tests. Additionally, effect sizes (d) were calculated and significance was set at p ≤ 0.05. Oral contraceptive users had reduced lumbar spine areal bone mineral density after adjustment for Total BPAQ (d = 0.58; p = 0.041) compared with non-OC users, but all other total body and site-specific DXA measures of bone mineral content and density were similar between contraceptive and injury groups. When comparing bone geometry of the 4, 38, and 66% tibiae using pQCT, no significant differences were found after adjustment for either contraceptive or injury group (all p > 0.060). Our findings suggest that OC usage was not associated with reduced skeletal health in competitive female rowers as evidenced by all Z-scores being above −2.0 and similar bone indices of mineralized content, density, geometry, and estimated strength between the groups.
Robust linear DNA degradation supports replication–initiation-defective mutants in Escherichia coli

Abstract RecBCD helicase/nuclease supports replication fork progress via recombinational repair or linear DNA degradation, explaining recBC mutant synthetic lethality with replication elongation defects. Since replication initiation defects leave chromosomes without replication forks, these should be insensitive to the recBCD status. Surprisingly, we found that both Escherichia coli dnaA46(Ts) and dnaC2(Ts) initiation mutants at semi-permissive temperatures are also recBC-colethal. Interestingly, dnaA46 recBC lethality suppressors suggest underinitiation as the problem, while dnaC2 recBC suppressors signal overintiation. Using genetic and physical approaches, we studied the dnaA46 recBC synthetic lethality, for the possibility that RecBCD participates in replication initiation. Overproduced DnaA46 mutant protein interferes with growth of dnaA+ cells, while the residual viability of the dnaA46 recBC mutant depends on the auxiliary replicative helicase Rep, suggesting replication fork inhibition by the DnaA46 mutant protein. The dnaA46 mutant depends on linear DNA degradation by RecBCD, rather than on recombinational repair. At the same time, the dnaA46 defect also interacts with Holliday junction-moving defects, suggesting reversal of inhibited forks. However, in contrast to all known recBC-colethals, which fragment their chromosomes, the dnaA46 recBC mutant develops no chromosome fragmentation, indicating that its inhibited replication forks are stable. Physical measurements confirm replication inhibition in the dnaA46 mutant shifted to semi-permissive temperatures, both at the level of elongation and initiation, while RecBCD gradually restores elongation and then initiation. We propose that RecBCD-catalyzed resetting of inhibited replication forks allows replication to displace the “sticky” DnaA46(Ts) protein from the chromosomal DNA, mustering enough DnaA for new initiations.
Lactic acid bacteria: prominent player in the fight against human pathogens

ABSTRACT Introduction The human microbiome is a unique repository of diverse bacteria. Over 1000 microbial species reside in the human gut, which predominantly influences the host’s internal environment and plays a significant role in host health. Lactic acid bacteria have long been employed for multiple purposes, ranging from food to medicines. Lactobacilli, which are often used in commercial food fermentation, have improved to the point that they might be helpful in medical applications. Areas covered This review summarises various clinical and experimental evidence on efficacy of lactobacilli in treating a wide range of infections. Both laboratory based and clinical studies have been discussed. Expert opinion Lactobacilli are widely accepted as safe biological treatments and host immune modulators (GRAS- Generally regarded as safe) by the US Food and Drug Administration and Qualified Presumption of Safety. Understanding the molecular mechanisms of lactobacilli in the treatment and pathogenicity of bacterial infections can help with the prediction and development of innovative therapeutics aimed at pathogens which have gained resistance to antimicrobials. To formulate effective lactobacilli based therapy significant research on the effectiveness of different lactobacilli strains and its association with demographic distribution is required. Also, the side effects of such therapy needs to be evaluated.
A Capacitive Electrocardiography System With Dedicated Noise-Cancellation Algorithms for Morphological Analysis

<italic>Objective:</italic> Capacitive electrocardiography (cECG) has been proposed for ambulatory cardiac health monitoring. However, due to the high sensitivity of capacitive electrodes to various noise sources, particularly the power-line interference (PLI) and motion artifacts (MA), the existing capacitive systems are only verified in terms of RR interval. The aim of the present study is to explore the feasibility of using cECG for morphological analysis, and thus to extract clinical meaningful parameters. <italic>Methods:</italic> A capacitive electrode with active guarding is realized. A phase-locked-loop (PLL) based adaptive canceller is employed to remove PLI from the cECG. Wavelet analysis is adopted to cancel other noises. The developed capacitive system and algorithms are evaluated by real ECG measurements on 7 volunteers using 3-lead configuration. The correlation coefficient (CC) between the processed cECG and the wet ECG is calculated in two different conditions: with and without the QRS complex. Several frequently used diagnostic parameters, i.e., RR interval, QRS interval, P segment, T segment, ST segment, are extracted and compared with that obtained from the wet ECG. <italic>Results:</italic> High CCs are observed between the cECG and the wet ECG in both conditions, i.e., <inline-formula><tex-math notation="LaTeX">$0.97\pm 0.03$</tex-math></inline-formula> with the QRS complex and <inline-formula><tex-math notation="LaTeX">$0.92\pm 0.07$</tex-math></inline-formula> without the QRS complex. Besides, RR interval extracted from the two different ECG signals are identical for each subject. Other diagnostic parameters are quite similar. <italic>Significance:</italic> Our results suggest cECG to be reliable for ambulatory heart rate monitoring. The results also indicate the feasibility of using cECG for clinical diagnosis.
Emerging patterns of fluoroquinolone resistance in Campylobacter jejuni in the UK [1998–2018]

Campylobacter jejuni (C.jejuni) is the most common causative agent of bacterial food poisoning worldwide and is known to be genetically highly diverse. C. jejuni is increasingly resistant to fluoroquinolone antibiotics, but very few studies have investigated variant-specific patterns of resistance across time. Here we use statistical modelling and clustering techniques to investigate patterns of fluoroquinolone resistance amongst 10,359 UK isolates from human disease sampled over 20 years. We observed six distinct patterns of fluoroquinolone sensitivity/resistance in C. jejuni across time, grouping by clonal complex (CC). Some CCs were fully resistant, some shifted from susceptible to resistant following a sigmoidal shape, and some remained susceptible over time. Our findings indicate that the fluoroquinolone resistance patterns of C. jejuni are complicated and cannot be analysed as a single species but divided into variant dynamics so that the factors driving resistance can be thoroughly investigated.
Quantum interference of edge supercurrents in a two-dimensional topological insulator

Josephson weak links made of two-dimensional topological insulators (TIs) exhibit magnetic oscillations of the supercurrent that are reminiscent of those in superconducting quantum interference devices (SQUIDs). We propose a microscopic theory of this effect that goes beyond the approaches based on the standard SQUID theory. For long junctions we find a temperature-driven crossover from Phi_0-periodic SQUID-like oscillations to a 2 Phi_0-quasiperiodic interference pattern with different peaks at even and odd values of the magnetic flux quantum Phi_0=ch/2e. This behavior is absent in short junctions where the main interference signal occurs at zero magnetic field. Both types of interference patterns reveal gapless (protected) Andreev bound states. We show, however, that the usual sawtooth current-flux relationship is profoundly modified by a Doppler-like effect of the shielding current which has been overlooked previously. Our findings may explain recently observed even-odd interference patterns in InAs/GaSb-based TI Josephson junctions and uncover unexplored operation regimes of nano-SQUIDs.
Predicting intentions to get the COVID‐19 vaccine in India: An integration of theory of planned behaviour and health belief model

Abstract The aim of the study was to determine intention to receive Coronavirus disease‐19 (COVID‐19) vaccination in India. An integrated model of Theory of Planned behaviour (TPB) and Health Belief Model (HBM) was used. Online survey was conducted in Delhi NCR region to find out the intention to receive vaccination. Data was collected from 845 respondents during 15 February 2021–15 March 2021, just after COVID‐19 vaccination became available in the India. Partial Least Square‐Structural Equation Modelling was used to establish the relationships. The results of the analysis show that proposed framework is significant and explained 71% variance in intentions. Attitude, subjective norm, perceived behaviour control and self‐efficacy from the TPB had direct (or significant independent) effects on intention and perceived benefits, perceived susceptibility from the HBM have indirect effect on the intentions through attitude. The present study can be used to maximise the uptake of vaccination in any future pandemic. This theoretically‐driven model can be used to guide health policy makers and health care providers to increase the uptake of vaccination.
CD69 flow cytometry to complement interferon‐γ release assay for active tuberculosis

The interferon‐γ (IFN‐γ) release assay (IGRA) is widely used to diagnose tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb). However, indeterminate IGRA results due to “high Nil” or “low PHA” responses limit its clinical utility. We developed a novel assay using CD69 flow cytometry (FC) to complement IGRA.
[Prevalence and lethality of interstitial pulmonary diseases in the Region of Los Ríos, Chile].

BACKGROUND Idiopathic Pulmonary Fibrosis (IPF) is a subtype of interstitial lung disease (ILD) that significantly affects people's quality of life, whose prevalence and mortality has increased. AIM To determine the prevalence and lethality of ILD in the Los Ríos Region between the years 2018 and 2019. MATERIAL AND METHODS Review of a database of all patients attended at an outpatient clinic of the regional hospital. Those patients with the diagnosis of ILD and seen between 2018 and 2019 were selected. Mortality and its causes were verified with death certificates. RESULTS In the study period, 339 cases with ILD, aged 71 ± 10 years (64% women) were identified. The calculated ILD prevalence was 84 cases per 100,000 inhabitants, with a higher predominancy in Futrono and Paillaco communes. IPF and Connective Tissue Diseases were the predominant subtypes. Overall lethality was 18%, with more deaths among patients with IPF (n = 31). CONCLUSIONS In the Los Ríos Region, ILDs have a relevant prevalence and lethality.
Intentions to Steal and the Commitment Problem. The Role of Moral Emotions and Self-Serving Justifications

This study focuses on determinants underlying young persons” self-reported intentions to steal a small amount of money. From an evolutionary standpoint, theft is a frequency-dependent strategy that may have been favored because it gave individuals a reproductively-relevant advantage in the competition for scarce resources. Although human groups do not tolerate the act of stealing, theft is still very common. Our study is rooted in Robert Frank's theory of the moral commitment problem. Moral emotions such as anticipated guilt are devices designed by evolutionary forces to motivate cooperative behavior in situations entailing a commitment problem. However, the anticipation of guilt feelings can be circumvented by self-serving justifications, therefore increasing the likelihood to steal. A large region-wide sample of adolescents (N = 3694) is used to analyze whether anticipated moral guilt and self-serving justifications mediate the effects of empathy, fear sensitivity, and perceived peer disapproval in their relationship to intentions to steal. Several propositions are tested in a latent variable model within the framework of SEM. Visual scenarios depicting an opportunity to take a small amount of money from a stranger are used to elicit participants” self-reported intentions to steal. Results suggest that empathic concern and empathic perspective-taking, perceived peer disapproval, and fear sensitivity affect the likelihood of theft by influencing anticipated guilt and self-serving justifications that, in turn, respectively reduce and promote the likelihood of theft.
The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function

Abstract Equal partitioning of the multi-copy 2-micron plasmid of the budding yeast Saccharomyces cerevisiae requires association of the plasmid Rep1 and Rep2 proteins with the plasmid STB partitioning locus. Determining how the Rep proteins contribute has been complicated by interactions between the components. Here, each Rep protein was expressed fused to the DNA-binding domain of the bacterial repressor protein LexA in yeast harboring a replication-competent plasmid that had LexA-binding sites but lacked STB. Plasmid transmission to daughter cells was increased only by Rep2 fusion expression. Neither Rep1 nor a functional RSC2 complex (a chromatin remodeler required for 2-micron plasmid partitioning) were needed for the improvement. Deletion analysis showed the carboxy-terminal 65 residues of Rep2 were required and sufficient for this Rep1-independent inheritance. Mutation of a conserved basic motif in this domain impaired Rep1-independent and Rep protein/STB-dependent plasmid partitioning. Our findings suggest Rep2, which requires Rep1 and the RSC2 complex for functional association with STB, directly participates in 2-micron plasmid partitioning by linking the plasmid to a host component that is efficiently partitioned during cell division. Further investigation is needed to reveal the host factor targeted by Rep2 that contributes to the survival of these plasmids in their budding yeast hosts.
Attenuated Cardiovascular Reactivity to Acute Psychological Stress Predicts Future Fatigue Symptoms in Truck Drivers

Our study demonstrated attenuated cardiovascular reactivity is predictive of future fatigue in truck drivers, who are a safety critical workforce. Attenuated responses to stress may have negative consequences for health and for driving safety, and cardiovascular stress reactivity could identify drivers who may be at heightened risk for developing fatigue. Objectives This study aimed to investigate the impact of a 6-month health intervention on truck drivers' cardiovascular reactivity to stress and whether cardiovascular reactivity was predictive of depression, anxiety, or fatigue symptoms at 6-month follow-up. Methods Two hundred thirty-eight truck drivers completed a 6-month cluster randomized controlled trial to increase physical activity and completed a stress protocol (Stroop and mirror-tracing tasks) with measurements of heart rate, systolic blood pressure, and diastolic blood pressure taken, alongside fatigue, anxiety, and depressive symptoms assessment. Measures were taken at 0 and 6 months. Results Analyses showed a negative relationship between 0-month diastolic blood pressure reactivity and 6-month persistent fatigue. Trends toward negative relationships between systolic blood pressure reactivity and future anxiety and fatigue symptoms at 6 months were evident. Conclusions Our findings may have serious implications, as fatigue can be a major cause of road traffic collisions in truck drivers.