Miracle-dz/UniFAR-FTUs huggingface dataset:
Defining cardiac cell populations and relative cellular composition of the early fetal human heart

The human heart is primarily composed of cardiomyocytes, fibroblasts, endothelial and smooth muscle cells. Reliable identification of fetal cardiac cell types using protein markers is important for understanding cardiac development and delineating the cellular composition of the human heart during early development, which remains largely unknown. The aim of this study was to use immunohistochemistry (IHC), flow cytometry and RT-PCR analyses to investigate the expression and specificity of commonly used cardiac cell markers in the early human fetal heart (8-12 post-conception weeks). The expression of previously reported protein markers for the detection of cardiomyocytes (Myosin Heavy Chain (MHC) and Troponin I (cTnI)), fibroblasts (DDR2, Thy1, Vimentin), endothelial cells (CD31) and smooth muscle cells (α-SMA) were assessed. Flow cytometry revealed two distinct populations of cTnI expressing cells based on fluorescence intensity: cTnIHigh and cTnILow. MHC positive cardiomyocytes were cTnIHigh, whereas MHC negative non-myocyte cells were cTnILow. cTnI expression in non-myocytes was further confirmed by IHC and RT-PCR analyses, suggesting troponins are not cardiomyocyte-specific and may play distinct roles in non-muscle cells during early development. Vimentin was confirmed to be enriched in cultured fibroblast populations and flow cytometry revealed VimHigh and VimLow cell populations in the fetal heart. MHC positive cardiomyocytes were VimLow whilst CD31 positive endothelial cells were VimHigh. Based on the markers investigated, we estimate fetal human cardiomyocyte populations comprise 75-80% of total cardiac cells and exhibit the following marker profile: α-MHC+/cTnIHigh/VimLow. For the non-cardiomyocyte population, we estimate they comprise 20-25% of total cardiac cells and exhibit the following marker profile: α-MHC-/cTnILow/VimHigh. Our study suggests the marker profiles and proportions of fetal cardiac populations are distinct from that of the adult heart.
Evaluation of a pedagogical e-tool for promoting students’ well-being: The perspective of future prevention providers

The aim of the present study was to evaluate the digitally supported pedagogical tool for promoting students' well-being, developed during Erasmus+ project. The research includes the data from 59 fourth-year students in a five-year MA degree program in psychology (50 women and 9 men) aged 22–46 (M = 26.51, SD = 5.43). We used a survey questionnaire developed for the purposes of the study to assess the attractiveness and usefulness of the e-tool, the effectiveness of the different forms of communication used in the tool, and its overall strengths and weaknesses. The mean overall satisfaction with the tool was high. Among the factors contributing to the overall satisfaction with the tool, the content of the tool and the attractiveness of its message form was rated the highest. Online videos were considered to be the most effective form of communication used in the tool. The most appreciated features of the tool were the use of multimedia and remote resources and an attractive form; the weakest side of the tool, according to the respondents, turned out to be its appropriateness for the recipients’ developmental stage. Erasmus+ projects can develop valuable outcomes for school practice. They have also the potential for educating future staff working in various areas of psychological practice. Future psychologists and prevention providers should come into contact with the international nature of education, psychoeducation, and modern prophylaxis already during their studies.
The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage

Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many Brassicaceae family plants, including Chinese cabbage (Brassica rapa ssp. pekinensis), produce a unique glucosinolate (GSL) secondary metabolite, which enhances plant protection, facilitates the design of functional foods, and has potential medical applications (e.g., as antidiabetic and anticancer agents). The levels of GSLs change diurnally, suggesting a connection to the circadian clock system. We investigated whether circadian clock genes affect the biosynthesis of GSLs in Brassica rapa using RNAi-mediated suppressed transgenic Brassica rapa GIGENTEA homolog (BrGI knockdown; hereafter GK1) Chinese cabbage. GIGANTEA plays an important role in the plant circadian clock system and is related to various developmental and metabolic processes. Using a validated GK1 transgenic line, we performed RNA sequencing and high-performance liquid chromatography analyses. The transcript levels of many GSL pathway genes were significantly altered in GK1 transgenic plants. In addition, GSL contents were substantially reduced in GK1 transgenic plants. We report that the BrGI circadian clock gene is required for the biosynthesis of GSLs in Chinese cabbage plants.
The Dynamism of Transposon Methylation for Plant Development and Stress Adaptation

Plant development processes are regulated by epigenetic alterations that shape nuclear structure, gene expression, and phenotypic plasticity; these alterations can provide the plant with protection from environmental stresses. During plant growth and development, these processes play a significant role in regulating gene expression to remodel chromatin structure. These epigenetic alterations are mainly regulated by transposable elements (TEs) whose abundance in plant genomes results in their interaction with genomes. Thus, TEs are the main source of epigenetic changes and form a substantial part of the plant genome. Furthermore, TEs can be activated under stress conditions, and activated elements cause mutagenic effects and substantial genetic variability. This introduces novel gene functions and structural variation in the insertion sites and primarily contributes to epigenetic modifications. Altogether, these modifications indirectly or directly provide the ability to withstand environmental stresses. In recent years, many studies have shown that TE methylation plays a major role in the evolution of the plant genome through epigenetic process that regulate gene imprinting, thereby upholding genome stability. The induced genetic rearrangements and insertions of mobile genetic elements in regions of active euchromatin contribute to genome alteration, leading to genomic stress. These TE-mediated epigenetic modifications lead to phenotypic diversity, genetic variation, and environmental stress tolerance. Thus, TE methylation is essential for plant evolution and stress adaptation, and TEs hold a relevant military position in the plant genome. High-throughput techniques have greatly advanced the understanding of TE-mediated gene expression and its associations with genome methylation and suggest that controlled mobilization of TEs could be used for crop breeding. However, development application in this area has been limited, and an integrated view of TE function and subsequent processes is lacking. In this review, we explore the enormous diversity and likely functions of the TE repertoire in adaptive evolution and discuss some recent examples of how TEs impact gene expression in plant development and stress adaptation.
Design and Analysis of Dual‐Stator Brushless Doubly‐Fed Generator for Wind Turbine

Brushless doubly‐fed generator (BDFG) is a most promising candidate for the wind turbine due to its simple structure, high reliability, and low converter capacity. To improve space utilization and achieve higher power density, this paper proposes a new dual‐stator BDFG (DS‐BDFG), which provides two separated windings, namely, the power winding (PW) placed in the outer stator and the control winding (CW) placed in the inner stator. A cup‐shaped rotor sandwiched between the inner and outer stators consists of the short‐circuited coil and the salient‐pole core. The coupling between the PW and the CW is realized via the rotor based on the general airgap magnetic field modulation theory for electrical machines. A prototype of the DS‐BDFG with rated PW power of 7.2 kW is designed and optimized. The keys of this paper are the power equation derivation, the determination of pole/slot combinations, and the rotor optimization. The electromagnetic characteristics and unbalanced magnetic force of the DS‐BDFG are analyzed by 2D finite element analysis to verify the reasonableness of the proposed model. © 2021 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC.
Impact of Induction Furnace Steel Slag as Replacement for Fired Clay Brick Aggregate on Flexural and Durability Performances of RC Beams

This research investigates the flexural and durability performances of reinforced concrete (RC) beams made with induction furnace steel slag aggregate (IFSSA) as a replacement for fired clay brick aggregate (FCBA). To achieve this, 27 RC beams (length: 750 mm, width: 125 mm, height: 200 mm) were made with FCBA replaced by IFSSA at nine replacement levels of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 80%, and 100% (by volume). Flexural tests of RC beams were conducted by a four-point loading test, where the deflection behavior of the beams was monitored through three linear variable displacement transducers (LVDT). The compressive strength and durability properties (i.e., porosity, resistance to chloride ion penetration, and capillary water absorption) were assessed using the same batch of concrete mix used to cast RC beams. The experimental results have shown that the flexural load of RC beams made with IFSSA was significantly higher than the control beam (100% FCBA). The increment of the flexural load was proportional to the content of IFSSA, with an increase of 27% for the beam made with 80% IFSSA than the control beam. The compressive strength of concrete increased by 56% and 61% for the concrete made with 80% and 100% IFSSA, respectively, than the control concrete, which is in good agreement with the flexural load of RC beams. Furthermore, the porosity, resistance to chloride ion penetration, and capillary water absorption were inversely proportional to the increase in the content of IFSSA. For instance, porosity, chloride penetration, and water absorption decreased by 43%, 54%, and 68%, respectively, when IFSSA entirely replaced FCBA. This decreasing percentage of durability properties is in agreement with the flexural load of RC beams. A good linear relationship of porosity with chloride penetration resistance and capillary water absorption was observed.
Viral Infection Drives the Regulation of Feeding Behavior Related Genes in Salmo salar

The feeding behavior in fish is a complex activity that relies on the ability of the brain to integrate multiple signals to produce appropriate responses in terms of food intake, energy expenditure, and metabolic activity. Upon stress cues including viral infection or mediators such as the proinflammatory cytokines, prostaglandins, and cortisol, both Pomc and Npy/Agrp neurons from the hypothalamus are stimulated, thus triggering a response that controls both energy storage and expenditure. However, how appetite modulators or neuro-immune cues link pathogenesis and energy homeostasis in fish remains poorly understood. Here, we provide the first evidence of a molecular linkage between inflammation and food intake in Salmon salar. We show that in vivo viral challenge with infectious pancreatic necrosis virus (IPNV) impacts food consumption by activating anorexic genes such as mc4r, crf, and pomcb and 5-HT in the brain of S. salar. At the molecular level, viral infection induces an overall reduction in lipid content in the liver, favoring the production of AA and EPA associated with the increment of elovl2 gene. In addition, infection upregulates leptin signaling and inhibits insulin signaling. These changes are accompanied by a robust inflammatory response represented by the increment of Il-1b, Il-6, Tnfa, and Pge2 as well as an increased cortisol level in vivo. Thus, we propose a model in which hypothalamic neurons respond to inflammatory cytokines and stress-related molecules and interact with appetite induction/inhibition. These findings provide evidence of crosstalk between pathogenesis-driven inflammation and hypothalamic–pituitary–adrenocortical axes in stress-induced food intake behavior in fish.
Může Bayesův faktor nahradit P hodnotu?

The P value was introduced as a value to evaluate the results of statistical tests. The basic concept originated in the 1920s, and after the Second World War it was significantly expanded. For about the last three decades there has been intense discussion about the problematic features of the P value concept and its use in science, and voices calling to abolish use of the P value are growing louder. In addition, suggestions have been made for alternative procedures that could replace or supplement the P value. Statisticians have tried to invent an indicator similar to the P value, but without its weaknesses. There are many of these options. Besides alternatives within the classical statistical testing paradigm, the use of an alternative statistical approach, so-called Bayesian statistics, is increasingly being discussed. An example of a moderate recommendation is that of using the Bayes factor, essentially an analogue of the P value in the Bayesian world. The aim of this article is to present the Bayes factor in detail, to describe its similarities and dissimilarities with the P value, and discuss the possibilities of its calculation. In addition to computational procedures, a detailed discussion of the weaknesses of the Bayes factor is also included.
RETRACTED ARTICLE: Nexus between sustainable economic growth and foreign private investment: evidence from emerging and developed economies

We, the Editors and Publisher of the Journal of Sustainable Finance and Investment, have retracted the following article: Nexus between sustainable economic growth and foreign private investment: evidence from emerging and developed economies, DOI: 10.1080/20430795.2021. 1990834 Subsequent to publication it has been determined that the article contains significant textual overlap with: Nexus between economic growth and foreign private investment: Evidence from Pakistan economy, DOI: 10.1080/23322039.2021. 1956067 which was not cited or otherwise acknowledged. When approached for an explanation the following authors reported that they contributed to the article at the revision stage and were unaware of the overlap with the previously published article: Muhammad Safdar Sial, Jacob Cherian, Susana Alvarez-Otero, Ubaldo Comite, Stefan Gunnlaugsson, and Mosab Tabash. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as ‘Retracted’.
Association between Dietary Habits and Type 2 Diabetes Mellitus in Yangon, Myanmar: A Case–Control Study

In Myanmar, the escalating prevalence of type 2 diabetes mellitus (T2DM) and impaired glucose tolerance among adults was recently reported, with the highest prevalence in the Yangon Region. The aim of the present study was to identify the risk factors in dietary habits and their relationship with T2DM in urban Myanmar residents. We conducted a case–control study recruiting 300 individuals aged 25–74 years living in the Yangon Region, consisting of 150 newly diagnosed cases attending a diabetes clinic, and 150 controls, who were community residents and free of diabetes. The case group had a significantly higher consumption of noodles, fish, beans, fermented food and pickles, dried food, topping seasonings, and non-dairy milk products than the control group, whereas they had a lower vegetable intake (more than three servings/day) and fruit intake (more than three servings/day) than the control group. Furthermore, the case group exhibited a higher frequency of some dietary behaviors than the control group, such as (1) having meals with family, (2) skipping breakfast, and (3) eating out. The final model showed that topping seasonings (adjusted odds ratio (aOR) 11.23, 95% confidence interval (CI) 3.08–40.90), more than three servings/day of vegetable intake (aOR 0.18, 95% CI 0.05–0.67), and having meals with family (aOR 2.23, 95% CI 1.05–4.71) were associated with diabetes. The study suggests that Myanmar’s characteristic dietary culture of topping their meals with salty seasonings and sauces and eating multiple dishes together as a family are risk factors associated with T2DM. Our findings may contribute recommendations and opportunities for the primary prevention of T2DM in urban Myanmar.
Discretionary stock halt and analyst forecast

ABSTRACT The increasing number of discretionary stock halts, has attracted the attention of regulatory authorities and scholars. They have also become a significant obstacle to the internationalization of China's capital market. This article systematically examines the impact of discretionary halts on the information environment from the perspective of analysts. The study finds that discretionary halts lead to a decrease in the number of analysts following, a decline in analysts' forecast accuracy, and an increase in analysts' forecast dispersion. Further research finds that reducing the amount and the decline of the quality of information disclosure are two crucial channels for discretionary stock halts deteriorating the information environment. Cross-sectional tests show that the impact of discretionary stock halts is more pronounced in samples with lower investor protection and higher agency costs. Above results indicate that the discretionary halt deteriorates the company's information environment, which provides important policy implications for China's capital market reform.
RELIEF: A Digital Health Tool for the Remote Self-Reporting of Symptoms in Patients with Cancer to Address Palliative Care Needs and Minimize Emergency Department Visits

The lack of timely symptom reporting remains a barrier to effective symptom management and comfort for patients with cancer-related palliative care needs. Poor symptom management at home can lead to unwanted outcomes, such as emergency department visits and death in hospital. We developed and evaluated RELIEF, a remote symptom self-reporting app for community patients with palliative care needs. A pilot feasibility study was conducted at a large, community hospital in Ontario, Canada. Patients self-reported their symptoms each morning using validated clinical symptom measures and RELIEF would alert for worsening or severe symptoms. RELIEF alerts were monitored by palliative care nurses who would then contact patients to determine if appropriate clinical intervention could be initiated to avoid unnecessary emergency department visits. A total of 20 patients were recruited to use RELIEF for two months. Patients completed 80% of daily self-report assessments; 133 alerts were trigged, half of which required clinical intervention. No patient visited the emergency department for symptom management during the study. Clinical staff estimated five emergency department visits were avoided because of RELIEF—saving an estimated cost of over CAD 60,000. RELIEF is a feasible and acceptable method for the remote monitoring of patients with palliative care needs through regular symptom self-reporting.
Assessment of Soil Carbon Dioxide Efflux and its Controlling Factors in Moist Temperate Forest of West Himalayas

In this study, the soil CO2 efflux was measured by closed dynamic system method along with soil and meteorological parameters at 1600, 1700 and 1800 m elevations along different directional-aspects over a period of one year. The annual CO2 efflux rate (Fc) varied from 1.02 to 22.57 μmol m sec, which was highest in the rainy season. The annual average Fc was maximum (8.67 μmol m sec) at east facing slope followed by 7.58 and 7.32 μmol m sec at south facing slope and north facing slope respectively. Temperature (Ts), moisture (Sm) and evaporation of soil were found to be significant variables and selected to develop the regression model with R value of 0.85. The effect of soil moisture on Fc above 15°C Ts exhibited a better relationship with R value of 0.48 and temperature sensitivity (Q10) was found 3.25. This study reveals that the key controlling factors of CO2 efflux rate are soil moisture and soil temperature, which explains 66% variation in soil CO2 efflux.
The trade-off between information and pathogen transmission in animal societies

Social structure can regulate information and pathogen transmission via social contact or proximity, which ultimately affects individual fitness. In theory, the same network properties that favor social information transmission also favor the spread of socially-transmitted pathogens, creating a trade-off between them. The mechanisms underlying the development and stability of individual relationships considering this trade-off remain underexplored. Here, we outline the evolutionary mechanisms of social transmission and hypothesize that network topology can be optimized in a way that balances the costs and benefits of social relationships. In this context, emergent network properties might reflect a trade-off between information and pathogen transmission in animal societies. We then propose an implementation of Hinde’s classical framework by incorporating the costs of socializing in a negative feedback loop in the emergence of social structure. We hope this manuscript encourages research into this underxplored social trade-off and the evolutionary processes underlying it.
Secure and Energy Efficient Data Transaction in WSN through SSCHS Routing Protocol

Abstract: Wireless sensor networks are widely used due to its usage and advantages because it can utilize in mission critical tasks. One of the major issues in WSN is reliable data delivery without any loss and to increase network lifetime by utilizing energy efficient process. The objective of this work is to increase network lifetime at the same time ensuring high packet delivery ratio. Clustering is one of the best methods to increase network lifetime, however election process of cluster head will consume energy and reduces network performance. Therefore in proposed work, energy efficient cluster based routing protocol has been implemented which includes residual energy and distance as major parameter to form cluster. Cluster head selection will be a static process, once cluster is formed cluster head will be selected through election process after transaction the residual energy in CH will be checked with the threshold value and same CH will again act as head this reduces cluster formation and election process. In addition to provide secure data transaction MD5 algorithm has been implemented. Attack based data loss is also reduced and concentrated in proposed work to achieve objective of this work. Keywords: (SSCHS) Secure static cluster head selection, network lifetime, cluster, MD5 and Static cluster head.