Miracle-dz/UniFAR-FTUs huggingface dataset:
FBP1 /miR-24-1/enhancer axis activation blocks renal cell carcinoma progression via Warburg effect

Warburg effect is a pivotal hallmark of cancers and appears prevalently in renal cell carcinoma (RCC). FBP1 plays a negative role in Warburg effect as a rate-limiting enzyme in gluconeogenesis, yet its mechanism in RCC remains to be further characterized. Herein, we revealed that FBP1 was downregulated in RCC tissue samples and was related to the poor survival rate of RCC. Strikingly, miR-24-1 whose DNA locus is overlapped with enhancer region chr9:95084940-95087024 was closely linked with the depletion of FBP1 in RCC. Of note, miRNAs like miR-24-1 whose DNA loci are enriched with H3K27ac and H3K4me1 modifications are belonging to nuclear activating miRNAs (NamiRNAs), which surprisingly upregulate target genes in RCC through enhancer beyond the conventional role of repressing target gene expression. Moreover, miR-24-1 reactivated the expression of FBP1 to suppress Warburg effect in RCC cells, and subsequently inhibited proliferation and metastasis of RCC cells. In mechanism, the activating role of miR-24-1 was dependent on enhancer integrity by dual luciferase reporter assay and CRISPR/Cas9 system. Ultimately, animal assay in vivo validated the suppressive function of FBP1 on 786-O and ACHN cells. Collectively, the current study highlighted that activation of FBP1 by enhancer-overlapped miR-24-1 is capable of contributing to Warburg effect repression through which RCC progression is robustly blocked, providing an alternative mechanism for RCC development and as well implying a potential clue for RCC treatment strategy.
Thermogenic adipose tissue aging: Mechanisms and implications

Adipose tissue undergoes significant anatomical and functional changes with aging, leading to an increased risk of metabolic diseases. Age-related changes in adipose tissue include overall defective adipogenesis, dysfunctional adipokine secretion, inflammation, and impaired ability to produce heat by nonshivering thermogenesis. Thermogenesis in adipose tissue is accomplished by brown and beige adipocytes, which also play a role in regulating energy homeostasis. Brown adipocytes develop prenatally, are found in dedicated depots, and involute in early infancy in humans. In contrast, beige adipocytes arise postnatally in white adipose tissue and persist throughout life, despite being lost with aging. In recent years, there have been significant advances in the understanding of age-related reduction in thermogenic adipocyte mass and function. Mechanisms underlying such changes are beginning to be delineated. They comprise diminished adipose precursor cell pool size and adipogenic potential, mitochondrial dysfunction, decreased sympathetic signaling, and altered paracrine and endocrine signals. This review presents current evidence from animal models and human studies for the mechanisms underlying thermogenic adipocyte loss and discusses potential strategies targeting brown and beige adipocytes to increase health span and longevity.
Evaluation of the validity of the physical exercise peer support questionnaire for college students

Peer support for physical exercise is defined as behaviors such as mutual or one-way provision of material help and/or emotional care and companionship between peers in the physical environment and/or physical behavior. The assessment of peer support is complex and based on reasoning. Trustworthy assessment processes need to provide sufficient evidence of validity. The purpose of this study was to organize, collect, and use Kane's validity framework to provide validity evidence for the identification of peer support for physical exercise among college students. The article describes the experience of using the framework in this study, considers data related to the four inferences (scoring, generalization, extrapolation, and implication) that emerge from the assessment process. The findings of the study are then interpreted through the four inferences to determine whether this evidence supports the purpose of this study. Based on Kane's framework to explain the validity process of this study, the study concludes that the evidence in terms of scoring, generalization, extrapolation, and implication supports the use of the PEPSQ for the identification of physical exercise peer support among college students.
Evaluating the efficacy of virtual prenatal counseling for genitourinary anomalies

Introduction and Objectives The standardization of fetal sonography has enabled early detection of genitourinary anomalies and referral for prenatal counseling with pediatric urologists. Prenatal urologic consultation can determine the need for antenatal and postnatal intervention, assuage parental anxiety, and establish continuity of care. The COVID-19 pandemic led to a significant increase in virtual visits (VVs). Our aim was to characterize a cohort of patients who underwent prenatal VVs with pediatric urologists and to assess adherence to prenatal recommendations for neonatal urologic care. Materials and Methods Data were collected through retrospective chart review of all patients receiving prenatal virtual consultation for genitourinary anomalies at our institution from July 1, 2020 to September 30, 2021. Data collected include maternal and gestational age at the time of prenatal imaging and VV, diagnosis of the fetus and neonate, and adherence to pediatric urological recommendations. Results During the study period, there were 70 prenatal VVs for 69 singleton and 1 twin gestation. 84 prenatal genitourinary diagnoses were made in these 71 cases. Of the 56/71 children known to have been born by the time of analysis, there are 63 postnatal genitourinary diagnoses. Postnatal diagnoses were consistent (38/56, 68%) or partially consistent (16/56, 29%) with prenatal diagnoses in 54/56 neonates (96%). There was 100% adherence to the postnatal imaging and antibiotic recommendations. One patient failed to attend the postnatal visit and one patient is due for repeat postnatal imaging to determine the necessity of follow-up. Conclusions Prenatal VVs promoted effective counseling of genitourinary anomalies and were associated with impressive postnatal adherence to prenatal recommendations. This is, to our knowledge, the first study to assess the impact of prenatal virtual pediatric urological consultation on postnatal adherence to prenatal recommendations. Future study will benefit from survey of the patient VV experience and evaluation of the relative merits of virtual versus in-person prenatal visits in a prospective, randomized fashion.
Doppler shift compensation performance in Hipposideros pratti across experimental paradigms

A central aim of neuroethological research is to discover the mechanisms of natural behaviors in controlled laboratory studies. This goal, however, comes with challenges, namely the selection of experimental paradigms that allow full expression of natural behaviors. Here, we explore this problem in echolocating bats that evolved Doppler shift compensation (DSC) of sonar vocalizations to yield close matching between echo frequency and hearing sensitivity. We ask if behavioral tasks influence the precision of DSC in Pratt’s roundleaf bat, Hipposideros pratti, in three classic laboratory paradigms evoking audio-vocal adjustments: Stationary bats listening to echo playbacks, bats transported on a moving pendulum, and bats flying freely. We found that experimental conditions had a strong influence on the expression of the audiovocal frequency adjustments in bats. H. pratti exhibited robust DSC in both free-flying and moving-pendulum experiments but did not exhibit consistent audiovocal adjustments in echo playback experiments. H. pratti featured a maximum compensation magnitude of 87% and a compensation precision of 0.27% in the free flight experiment. Interestingly, in the moving pendulum experiment H. pratti displayed surprisingly high-precision DSC, with an 84% maximum compensation magnitude and a 0.27% compensation precision. Such DSC performance places H. pratti among the bat species exhibiting the most precise audio-vocal control of echo frequency. These data support the emerging view that Hipposiderid bats have a high-precision DSC system and highlight the importance of selecting experimental paradigms that yield the expression of robust natural behaviors.
The endogenous HBZ interactome in ATL leukemic cells reveals an unprecedented complexity of host interacting partners involved in RNA splicing

Adult T-cell leukemia/lymphoma (ATL) is a T-cell lymphoproliferative neoplasm caused by the human T-cell leukemia virus type 1 (HTLV-1). Two viral proteins, Tax-1 and HBZ play important roles in HTLV-1 infectivity and in HTLV-1-associated pathologies by altering key pathways of cell homeostasis. However, the molecular mechanisms through which the two viral proteins, particularly HBZ, induce and/or sustain the oncogenic process are still largely elusive. Previous results suggested that HBZ interaction with nuclear factors may alter cell cycle and cell proliferation. To have a more complete picture of the HBZ interactions, we investigated in detail the endogenous HBZ interactome in leukemic cells by immunoprecipitating the HBZ-interacting complexes of ATL-2 leukemic cells, followed by tandem mass spectrometry analyses. RNA seq analysis was performed to decipher the differential gene expression and splicing modifications related to HTLV-1. Here we compared ATL-2 with MOLT-4, a non HTLV-1 derived leukemic T cell line and further compared with HBZ-induced modifications in an isogenic system composed by Jurkat T cells and stably HBZ transfected Jurkat derivatives. The endogenous HBZ interactome of ATL-2 cells identified 249 interactors covering three main clusters corresponding to protein families mainly involved in mRNA splicing, nonsense-mediated RNA decay (NMD) and JAK-STAT signaling pathway. Here we analyzed in detail the cluster involved in RNA splicing. RNAseq analysis showed that HBZ specifically altered the transcription of many genes, including crucial oncogenes, by affecting different splicing events. Consistently, the two RNA helicases, members of the RNA splicing family, DDX5 and its paralog DDX17, recently shown to be involved in alternative splicing of cellular genes after NF-κB activation by HTLV-1 Tax-1, interacted and partially co-localized with HBZ. For the first time, a complete picture of the endogenous HBZ interactome was elucidated. The wide interaction of HBZ with molecules involved in RNA splicing and the subsequent transcriptome alteration strongly suggests an unprecedented complex role of the viral oncogene in the establishment of the leukemic state.
Odor-induced modification of oscillations and related theta-higher gamma coupling in olfactory bulb neurons of awake and anesthetized rats

Olfactory gamma oscillations (40–100 Hz) are generated spontaneously in animals and represent the activity of local olfactory bulb (OB) networks, which play important roles in cognitive mechanisms. In addition, high-frequency oscillations (HFO, 130–180 Hz) have attracted widespread attention and are novel neuronal oscillations with a frequency range closer to high gamma oscillations (60–100 Hz, HGOs). Both HGOs and HFOs are distinctly regulated by θ rhythm in the hippocampus. To understand their mediation mechanisms in the OB, we investigated whether local field potential (LFP) oscillations including HGOs and HFOs and even their coupling with theta rhythm are modified by odor stimulation in both freely moving and anesthetized rats. Therefore, we combined electrophysiological technology and cross-frequency coupling analysis approaches to determine the difference in the odor-modulated LFP oscillations between awake and anesthetized rats. The obtained results indicate that LFP oscillations including HGOs and HFOs were differently modified by odor stimulation in animals of both states. However, θ-HGO and θ-HFO coupling were modified in only awake animals. It is suggested that these oscillations and their interactions with theta oscillations may play crucial roles in olfactory network activity. This could pave the way for further understanding the underlying mechanisms of oscillations in OB neurons towards odor sensation.
An Eco-Friendly Fluidizable Fe x O y / CaO- γ -Al 2 O 3 Catalyst for Tar Cracking during Biomass Gasiﬁcation

: The present study deals with the development, characterization, and performance evaluation of an eco-friendly catalyst, using 2-methoxy-4-methylphenol (2M4MP) as a surrogate tar. The 2M4MP was selected due to its chemical functionalities and the fact that it is a good model compound to represent the tar formed during biomass low temperature gasiﬁcation. The eco-friendly catalyst was prepared using the typical Fe and Ca minerals which are present in ash. These ash components were added to a ﬂuidizable γ -Al 2 O 3 support using a multistep incipient impregnation, yielding Fe oxides as an active phase and CaO as the promoter. The prepared catalyst displayed a 120 m 2 / g BET speciﬁc surface area, with few γ -Al 2 O 3 bulk phase changes, as observed with XRD. TPD-NH 3 and pyridine FTIR allowed us to show the signiﬁcant inﬂuence of CaO reduced support acidity. A TPR analysis provided evidence of catalyst stability during consecutive reduction–oxidation cycles. Furthermore, catalyst evaluation vis- à -vis catalytic steam 2M4MP gasiﬁcation was performed using the ﬂuidized CREC riser simulator. The obtained results conﬁrm the high performance of the developed catalyst, with 2M4MP conversion being close to 100% and with selectivities of up to 98.6% for C1-C2 carbon-containing species, at 500 ◦ C, with a 7.5 s reaction time and 1.5 g steam / g 2M4MP. These high tar conversions are promising e ﬃ ciency indicators for alumina catalysts doped with Fe and Ca. In addition, the used catalyst particles could be blended with biochar to provide an integrated solid supplement that could return valuable mineral supplements to the soil.
A Class of Logic Functions Expressible by Polynomial-Size Binary Decision Diagrams

In this paper we discuss properties of logic functions expressible by BDD’s of feasible size. We deﬁne a class of logic functions expressible by BDD’s whose size (number of nodes) are bounded by a polynomial of the number of input variables. We derive some properties of this class through the discussion on the relation between polynomial-size BDD’s and Turing machines. We also focus on the relation between polynomial-size BDD’s and combinational circuits and show that polynomial size BDD’s can be synthesized into $O( \log^{2}n)$ depth combinational circuits
Adaptive Fuzzy Control of 2DoF Uncertain Robot Manipulator using Lyapunov Method Compared to RLSE

This paper presents an adaptive fuzzy logic modeling and control (AFLC) of the nonlinear systems associated with uncertainties. Feedback Linearization method (FBL), used to linear the proposed dynamic. Also to estimate the uncertain parts of the process, fuzzy logic systems are used. To speed up the learning of fuzzy systems with in-line or off-line of proposed algorithm, and also for reducing the error of the system against the unwanted changes and variation of the inputs parameters, recursive least square estimation (RLSE) method is used as an innovation in this paper . For comparing the robustness of this method against uncertainties, the already implemented recursive least square learning method is compared with another adaptive controller based on Lyapunov algorithm (MIT-Rule). Finally the effectiveness of the proposed approach is verified in identification and control of the two degrees of freedom manipulator with uncertainties showing great performance on achieving the targets.
Cellular and molecular mechanisms of colistin-induced nephrotoxicity

Background: Colistin is a polypeptide antibiotic extensively used as a last resort agent in the treatment of multi drug resistant (MDR) Gram-negative bacteria. Its bactericidal potency is associated with nephrotoxic adverse effects that limit the use in clinical practice. Little is known about the exact mechanism of colistin-induced cellular damage, and efficient protective strategies are lacking. Aim: To characterize the effect of colistin at the mitochondrial level and to explore the use of L-carnitine as a potential protective agent. Methods: The impact of colistin exposure on mitochondria was evaluated in intact wild type HEK293 cells (WT-HEK293 cells) and in freshly isolated mitochondria from both WT-HEK293 cells and mouse kidney. The impact of intracellular L-carnitine on colistin-induced mitochondrial toxicity was evaluated in HEK293 cells stably transfected with the low affinity L-carnitine transporter SLC22A4 (OCTN1). Results: We found that OCTN1-HEK293 cells had significantly higher intracellular levels of L-carnitine as compared with WT-HEK293 cells. After 48-hour exposure to colistin at an extracellular concentration of 100  M, a marked mitochondrial membrane depolarization was induced in WT-HEK293 cells whereas mitochondria from OCTN1-HEK293 cells were not affected. Mitochondria exposed to colistin were rapidly shrunken and depolarized. Mitochondrial membrane depolarization was fully abolished by ADP, the most potent inhibitor of the mitochondrial permeability transition pore (MPTP). L-carnitine, recently reported as an inhibitor of the MPTP, failed to directly protect from the depolarization. Conclusions: Colistin had a direct effect on mitochondrial function by inducing a rapid depolarization. The protective effect of ADP suggests that colistin-induced might be secondary to the opening of the MPTP. L-carnitine showed a protective effect towards mitochondria when cells were exposed to colistin, but
The 5 (cid:1) and 3 (cid:1) Downstream AUG Region Elements Are Required for Mosquito-Borne Flavivirus RNA Replication (cid:1)

Flaviviruses require complementarity between the 5 (cid:1) and 3 (cid:1) ends of the genome for RNA replication. For mosquito-borne ﬂaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3 (cid:1) DAR sequence is also part of a hairpin (HP-3 (cid:1) SL), and both complementarity between 5 (cid:1) and 3 (cid:1) DAR motifs and formation of the HP-3 (cid:1) SL in the absence of the 5 (cid:1) end are conserved among mosquito-borne ﬂaviviruses. Using West Nile virus as a model, we demonstrate that 5 (cid:1) –3 (cid:1) DAR complementarity and HP-3 (cid:1) SL formation are essential for viral RNA replication. Flaviviruses have an approximately 11-kb-long RNA genome of positive polarity (see reference 10). The viral RNA encodes one open reading frame (ORF), and the resulting polyprotein is cleaved co- and posttranslationally into 3 structural (N-terminal) and 7 nonstructural (NS) (C-terminal)
IN VITRO INHIBITORY EFFECTS OF MUCUNA PRURIENS AND VETIVERIA ZIZANOIDES ON SELECTED HUMAN BACTERIAL PATHOGENS

the chances of Therefore this study was undertaken to focus on the in vitro antimicrobial effects of two plants known for their medicinal value. Plant materials of Mucuna pruriens and Vetiveria zizanoides were tested for their antibacterial activities on selected strains of bacteria namely, Bacillus cereus (ATCC13061) Shigella flexneri (ATCC 12022) and Pseudomonas aeruginosa (ATCC 6643). These activities were compared with standard antibiotic namely Broad spectrum antibiotics, Penicillin
A Provision System for making Presentation Slides form documents

: In this paper, analyze a real fascinating task of always producing presentation slides for scholastic papers. The produced presentation slides can be utilized as drafts to offer the moderators some assistance with preparing their formal slides quickly. A novel framework called PPSGen is proposed to address this assignment. It first utilizes the relapse technique to take in the significance scores of the sentences in a scholarly paper, and after that endeavors the whole number straight programming (ILP) strategy to create all around organized slides by selecting and adjusting key expressions and sentences. Assessment results on a test set of 200 sets of papers and slides gathered on the web exhibit that our proposed PPSGen framework can produce slides with better quality. A client study is additionally shown to demonstrate that PPSGen has a couple of clear preferences over pattern techniques. And also we are enhancing the graphical elements into slides.
Role of mast cells and innate immune lymphoid (ILC2) cells in lung transplantation

The multifunctional role of mast cells (MCs) in the immune system is complex and has not fully been explored. MCs reside in tissues and mucous membranes such as the lung, digestive tract, and skin which are strategically located at interfaces with the external environment. These cells, therefore, will encounter external stimuli and pathogens. MCs modulate both the innate and the adaptive immune response in in ﬂ ammatory disorders including transplantation. MCs can have pro- and anti-in ﬂ ammatory functions, thereby regulating the outcome of lung transplantation through secretion of mediators that allow interaction with other cell types, particularly innate lymphoid cells (ILC2). ILC2 cells are a unique population of hematopoietic cells that coordinate the innate immune response against a variety of threats including infection, tissue damage, and homeostatic disruption. In addition, MCs can modulate alloreactive T cell responses or assist in T regulatory (Treg) cell activity. This paper outlines the current understanding of the role of MCs in lung transplantation, with a speci ﬁ c focus on their interaction with ILC2 cells within the engrafted organ. addition, IL-2 released from MCs induces tolerance in Treg cells and regulates transplantation survival. Activated ILC2s, MCs, and Th2 cells release Areg which is important in promoting EC repair from injury. Damaged epithelial cells, seen during transplant rejection, release IL-33 and ATP, which together can act on ILC2 and MCs to enhance their activity. A number of inhibitors including montelukast, IL-27, corticosteroid, PGI 2 , and lipoxin A4, can suppress the activation and/or proliferation of ILC2s and their release of in ﬂ ammatory mediators. epithelial cells; ILC2: innate