Miracle-dz/UniFAR-FTUs huggingface dataset:
Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin

Amaç: Ratlarda doksurubisin kaynaklı hepatotoksisite üzerine farklı dozlardaki timokinon ve resveratrolün (5 and 20 mg/kg) etkilerinin patolojik olarak değerlendirilmesi amaçlandı. Gereç ve Yöntem: Çalışmada 80 erkek Wistar Albino rat kullanıldı. Hayvanlar, kontrol (C, Fizyolojik tuzlu su, PO); doksorubisin (Fizyolojik tuzlu su, PO ve 10. günde 15mg/kg Dox, IP); Timokinon-5 (TQ-5, 5 mg/kg TQ, PO); TQ-20 (20 mg/kg TQ, PO); Resveratrol-5 (5 mg/kg Res, PO, günaşırı); Res-20 (20 mg/kg Res, PO); Dox+TQ-5; Dox+TQ-20; Dox+Res-5 ve Dox+Res-20 olmak üzere 10 gruba ayrıldı. 21 günlük deneme sonunda gruplardan rastgele 6 denek seçildi. Vücut ağırlığı tartıldıktan sonra, ötenazi uygulanan ratların karaciğerleri diseke edildi ve tartıldı. Karaciğer örneklerine rutin doku işleme işlemleri uygulandı. Mikroskobik olarak, hepatositlerde dejenerasyon, nekroz/apoptoz, safra kanalı hiperplazisi, disosiasyon, konjesyon, karyomegali, mononükleer hücre infiltrasyonu, çift çekirdekli hepatosit ve mitoz yönünden incelenerek her bir vaka için karaciğer total lezyon skoru hesaplandı. Bulgular: Dox uygulaması relatif karaciğer ağırlığını arttırırken, TQ ve Res gruplarında Dox kaynaklı artış önlendi (p<0.05). Dox ile artan karaciğer total lezyon skorunun TQ ve Res gruplarında daha düşük olduğu gösterildi (p<0.05). Ancak Dox+TQ-5, Dox+TQ-20 ve Dox+Res-20 grupları nekroz/apoptozda düzelme saptanmadı. Öneri: TQ ve Res (5 ve 20 mg/kg), Dox tarafından indüklenen total karaciğer lezyon skorunu azalttı. TQ ve Res, dejenerasyon ve inflamasyonu azaltmasına rağmen, anahtar kriterlerden biri olan nekroz/apoptoz üzerindeki zayıf koruyucu etkileri, kontrolsüz kullanımları için sınırlayıcı bir neden olarak kabul edildi.
Meta‐analysis of bacterial growth characteristics in platelet components: Refining the inputs of a simulation analysis comparing the relative safety of testing strategies

The relative safety of bacterial risk control strategies for platelets that include culture with or without rapid testing has been compared using simulation analysis. A wide range of bacterial lag and doubling times were included. However, published data on growth rates are available and these data have not been synthesized. We conducted a systematic review and meta‐analysis to estimate growth rates and used these estimates to refine a comparative safety analysis of bacterial risk control strategies in the FDA guidance
Diagnosis of the Accuracy of the Vehicle Scale Using Neural Network

Abstract The article describes a method for diagnosing the accuracy of the vehicle scale without using standard weights. The novel method defines the possibility to estimate whether the scale would pass the test for error of indication in the next verification or not, only by using the results from simple tests with load of estimated weight and appropriate classifier. The method is primarily developed for users of these scales. Created classifier is based on the neural network algorithm. The neural network was trained with data from verifications, which are provided by Slovak Legal Metrology. Well trained classifier can provide not only information whether the scale will potentially pass the mentioned test or not, but reliability which is associated with this result as well. In this way, the user has valuable information about the scale in the period between the verifications.
The impact of antenatal and postnatal indoor air pollution or tobacco smoke exposure on lung function at 3 years in an African birth cohort

Indoor air pollution (IAP) and tobacco smoke exposure (ETS) are global health concerns contributing to the burden of childhood respiratory disease. Studies assessing the effects of IAP and ETS in preschool children are limited. We assessed the impact of antenatal and postnatal IAP and ETS exposure on lung function in a South African birth cohort, the Drakenstein Child Health Study.
Does fetal growth restriction induce neuropathology within the developing brainstem?

Fetal growth restriction (FGR) is a complex obstetric issue describing a fetus that does not reach its genetic growth potential. The primary cause of FGR is placental dysfunction resulting in chronic fetal hypoxaemia, which in turn causes altered neurological, cardiovascular and respiratory development, some of which may be pathophysiological, particularly for neonatal life. The brainstem is the critical site of cardiovascular, respiratory and autonomic control, but there is little information describing how chronic hypoxaemia and the resulting FGR may affect brainstem neurodevelopment. This review provides an overview of the brainstem‐specific consequences of acute and chronic hypoxia, and what is known in FGR. In addition, we discuss how brainstem structural alterations may impair functional control of the cardiovascular and respiratory systems. Finally, we highlight the clinical and translational findings of the potential roles of the brainstem in maintaining cardiorespiratory adaptation in the transition from fetal to neonatal life under normal conditions and in response to the pathological environment that arises during development in growth‐restricted infants. This review emphasises the crucial role that the brainstem plays in mediating cardiovascular and respiratory responses during fetal and neonatal life. We assess whether chronic fetal hypoxaemia might alter structure and function of the brainstem, but this also serves to highlight knowledge gaps regarding FGR and brainstem development.
Neurotheranostics: The Next Frontier for Health Span

With an aging U.S. population, advancements in the treatment of Alzheimer disease (AD) and other neurodegenerative diseases are key to the maximization of health span. The recent approval of 2 antiamyloid antibodies, which decrease brain amyloid load, places us on the cusp of breakthrough therapies that target the mechanism of the disease rather than just treating the symptoms. Although the trials that led to these approvals studied patients with mild early symptoms, multiple ongoing trials have enrolled cognitively normal patients screened for AD biomarkers including risk factors for amyloid positivity, family history, and genetic markers. Thus, amyloid PET can help identify an at-risk population that can be enrolled for antiamyloid therapy to prevent AD symptoms from ever developing. In this review, we examine the paradigm of neurotheranostics and how PET biomarkers of amyloid, tau, inflammation, and neurodegeneration could characterize the pathologic stage of AD and therefore allow for personalized therapy.
Milk coagulation properties are moderately heritable in dairy cows: a meta-analysis using the random-effects model

Abstract This study aimed to conduct a meta-analysis using the random-effects model to merge published genetic parameter estimates for milk coagulation properties (MCP: comprising rennet coagulation time (RCT), curd-firming time (k20), curd firmness 30 min after rennet addition (a30), titrable acidity (TA) and milk acidity or pH) in dairy cows. Overall, 80 heritability estimates and 157 genetic correlations from 23 papers published between 1999 and 2020 were used. The heritability estimates for RCT, a30, k20, TA, and pH were 0.273, 0.303, 0.278, 0.189 and 0.276, respectively. The genetic correlation estimates between RCT-a30, RCT-pH, and RCT-TA were 0.842, 0.549 and −0.565, respectively. Genetic correlation estimates between RCT and production traits were generally low and ranged from −0.142 (between RCT and casein content) to 0.094 (between RCT and somatic cell score). Moderate and significant genetic correlations were observed between a30-pH (−0.396) and a30-TA (0.662). Also, the genetic correlation estimates between a30 and production traits were low to moderate and varied from −0.165 (between a30 and milk yield) to 0.481 (between a30 and casein content). Genetic correlation estimates between pH and production traits were low and varied from −0.190 (between pH and milk protein percentage) to 0.254 (between pH and somatic cell score). The results of this meta-analysis indicated the existence of additive genetic variation for MCP that could be used in genetic selection programs for dairy cows. Because of the moderate heritability of MCP and small genetic correlations with production traits, it could be possible to improve MCP with negligible correlated effects on production traits.
Effects of Short-Term Novice Archery Training on Reaching Movement Performance and Interlimb Asymmetries

Abstract Previous studies showed numerous evidence for the interlimb asymmetries in motor performance during arm reaching movements. Furthermore, these interlimb asymmetries have been shown to associate with spatial patterns of hand selection behavior. Importantly, these interlimb asymmetries can be modified systematically by occlusion of visual feedback, or a long-term sports training. In this study, we asked about the effects of a short-term training on interlimb asymmetries. Eighteen healthy young participants underwent a 12-week novice traditional archery training (TAT). Their unimanual dominant and nondominant arm reaching movement performance was assessed before and after TAT. We found that movement accuracy, movement precision, and movement efficiency in the experimental group have all improved significantly as a result of TAT. These improvements were comparable across both arms, thus the interlimb differences in movement performance were not affected by the short-term TAT and remained similar. These results suggest that while short-term training may contribute positively to reaching performance, it is unlikely to have a significant impact on the differences observed between the dominant and nondominant arms. This unique characteristics of dominant and nondominant arm should be taken into consideration when developing targeted sports and rehabilitation programs for athletes or individuals with acute or chronic motor deficits.
CXCL10 recruitment of γδ T cells into the hypoxic bone marrow environment leads to IL-17 expression and multiple myeloma progression.

In multiple myeloma (MM), bone marrow stromal cells (BMSCs) shape a unique niche within the bone marrow, promoting T-cell dysfunction and driving MM progression; however, the precise underlying mechanisms remain elusive. Here, we show that BMSC-mediated reprogramming of MM cells led to heightened production of CXCL10. CXCL10 orchestrated the recruitment of γδ T cells into the bone marrow, and this was observed in both the Vk*MYC and 5TGM1 mouse models of MM, as well as in patients experiencing refractory or relapsed MM. Furthermore, the dysfunctional γδ T cells in the MM bone-marrow niche exhibited increased PD-1 expression and IL-17 production. In the Vk*MYC mouse model, MM-associated bone lesions and mortality were markedly alleviated in Tcrd-/- mice, and MM disease progression could be rescued in these mice upon transplantation of γδ T cells expanded from wild-type mice, but not from Il17-/- mice. Mechanistically, the hypoxic microenvironment prevailing in the MM bone-marrow niche stimulated the expression of steroid receptor coactivator 3 (SRC-3) in γδ T cells, which in turn interacted with the transcriptional factor RORγt, promoting Il17 transcription. Pharmacological inhibition of SRC-3 utilizing SI-2 effectively suppressed Il17A expression in γδ T cells, leading to alleviation of MM progression in the murine models and enhancing the anti-MM efficacy of bortezomib. Our results illuminated the bone marrow microenvironment's involvement in provoking γδ T-cell dysfunction throughout MM progression and suggest SRC-3 inhibition as a promising strategy to enhance the effectiveness of immunotherapies targeting γδ T cells.
Sensors as a Service: Sensor network in the cloud

[CASTELLA] Este proyecto consiste en desarrollar e implementar un servicio, en la nube, de almacenamiento de datos procedentes de fuentes que se puedan cuantificar (tales como ritmo cardiaco, polucion del aire, o temperatura de un procesador) y mostrarlas en una pagina web, de forma sencilla y escalable.
Epinodosin suppresses the proliferation, invasion, and migration of esophageal squamous cell carcinoma by mediating miRNA‐143‐3p/Bcl‐2 axis

Epinodosin has shown antibacterial and antitumor biological characteristics in the documents. We found that Epinodosin has an effective inhibitory effect on esophageal squamous cell carcinoma (ESCC). However, the potential roles and mechanisms of Epinodosin in ESCC remain unclear. We performed many experiments to clarify the effect and mechanism of Epinodosin on ESCC. In this study, cell viability, invasion, migration, and apoptosis were determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,‐diphenytetrazoliumromide (MTT), Transwell, and flow cytometry. The differentially expressed miRNAs were screened through RNA transcriptome sequencing. The expression levels of miRNA‐143‐3p and some proteins were measured by real‐time polymerase chain reaction (PCR) and Western blot. The anticancer effects of Epinodosin in vivo were determined by a nude mouse model. Epinodosin suppressed cell proliferation/invasion/migration and induced ESCC cell apoptosis. Epinodosin remarkably affected the protein expression of mitogen‐activated protein kinase (MAPK) signaling pathway. The animal experiments demonstrated that Epinodosin could attenuate the growth of ESCC tumors in nude mice. The expression of p53, Bim, and Bax was upregulated, while that of Bcl‐2 was downregulated in tumor tissues. In conclusion, Epinodosin suppresses cell viability/invasion/migration, while induces ESCC cell apoptosis by mediating miRNA‐143‐3p and Bcl‐2, and can markedly attenuate the growth of ESCC tumors in nude mice.
Increased expression of human antiviral protein MxA in FUS proteinopathy in amyotrophic lateral sclerosis

Abstract FUS mutations are one of the major mutations in familial amyotrophic lateral sclerosis (ALS). The pathological hallmark is FUS‐positive neuronal cytoplasmic inclusions (FUS‐NCI), known as FUS proteinopathy. Human myxovirus resistance protein 1 (MxA) is an IFN‐induced dynamin‐like GTPase that acts as antiviral factor. In this study, we examined the expression of MxA in neurons bearing FUS‐NCI. We performed immunohistochemistry for FUS and MxA to examine the expression of MxA in two autopsy cases with different FUS gene mutations localized at the nuclear localization signal site (Case 1, H517P; Case 2, R521C). MxA. Most neurons bearing FUS‐NCI have increased cytoplasmic MxA expression. Increased cytoplasmic MxA showed several distribution patterns in relation to FUS‐NCIs such as the following: colocalization with NCI, distribution more widely than NCI, and different distribution peaks from NCI. Our results suggested that antiviral signaling IFNs are involved upstream in the formation of FUS‐NCI in ALS‐FUS patients.
Transmission dynamics of Human Rift Valley Fever virus in Ijara District, Garrissa county, Kenya

Error! Bookmark not defined. CHAPTER ONE: INTRODUCTION 1 1.1 Background Information 1 1.2 Statement of Problem 2 1.3 Justification 2 1.4 Research Questions 3 1.5 Null Hypothesis 4 1.6 Objectives 4 1.6. 1 General Objective 4 1.6. 2 Specific Objectives 4 CHAPTER TWO: LITERATURE REVIEW 5 2.1 Rift Valley Fever Virus 5 2.2 Epidemiology of RVF 6 2.3 Geographic distribution of RVF 7 2.4 Pathogenesis of RVF 8 2.4. 1 Pathology of RVF in humans 8
Cost-effectiveness analysis of pressure-controlled intermittent coronary sinus occlusion in elective percutaneous coronary intervention

ABSTRACT Objectives Percutaneous coronary intervention (PCI) represents the standard treatment for ST-elevated myocardial infarction, nevertheless, mortality and heart failures are frequent. Pressure-controlled intermittent coronary sinus occlusion (PiCSO) might reduce infarct size showing better patients’ outcomes. We evaluated the cost-effectiveness of PCI+PiCSO compared to PCI from the National Healthcare Service (NHS) perspective in Italy. Methods A Markov model was developed to estimate life years (LYs), quality-adjusted life years (QALYs) and costs. A micro-costing analysis has been performed to inform the cost of PCI+PiCSO procedure. Sensitivity analyses were performed to test the robustness of the model results. Results Considering a willingness-to-pay threshold of 50,000€/QALY for the ICUR and a cost for PCI+PiCSO procedure of 14,654€, the innovative strategy may be cost-effective compared to PCI alone from the Italian NHS perspective, showing an ICUR of 17,530€/QALY (ICER 14,631€/LY) over a lifetime horizon; the probabilistic sensitivity analysis showed that PCI+PiCSO is cost-effective in 78.8% of simulations.Considering the above mentioned willingness-to-pay threshold, PCI+PiCSO strategy would be cost-effective over a lifetime horizon considering a cost for PCI+PiCSO procedure lower than 28,160€. Conclusion PCI+PiCSO procedure may be considered a cost-effective technology that allows reducing cardiac events, while improving patients’ life expectancy and quality of life.
Social engagement and depressive symptoms mediate the relationship between age‐related hearing loss and cognitive status

Age‐related hearing loss (ARHL) is the third leading cause of years lived with disability. Connections among ARHL, depressive symptoms, social engagement and cognitive status are increasingly reported but the underlying mechanisms leading to these relationships are largely unknown. Exploring these mechanisms is a worthy goal, especially in older adults. This study aimed to examine the mediating effect of social engagement and depressive symptoms on the relationship between ARHL and cognitive status.
Trace Elements and Consequent Ecological Risks in Mining‐Influenced Streams of Appalachia

Appalachian (eastern USA) coal surface mines fracture geologic materials, causing release of both major ions and trace elements to water via accelerated weathering. When elevated above natural background, trace elements in streams may produce adverse effects on biota via direct exposure from water and sediment and via dietary exposure in food sources. Other studies have found elevated water concentrations of multiple trace elements in Appalachia's mining‐influenced streams. Except for Se, trace‐element concentrations in abiotic and biotic media of Appalachian mining‐influenced streams are less well known. We analyzed environmental media of headwater streams receiving alkaline waters from Appalachian coal mines for eight trace elements (Al, As, Cd, Cu, Ni, Sr, V, and Zn) and assessed the potential consequent ecological risks. Streamwater, particulate media (sediment, biofilm, leaf detritus), and benthic macroinvertebrates (primary consumers, secondary consumers, crayfish) were sampled from six mining‐influenced and three reference streams during low‐flow conditions in two seasons. Dissolved Cu, Ni, and Sr were higher in mining‐influenced streams than in reference streams; Ni, Sr, and Zn in fine sediments and Ni in macroinvertebrates were also elevated relative to reference‐stream levels in samples from mining‐influenced streams. Seasonal ratios of mining‐influenced stream concentrations to maximum concentrations in reference streams also demonstrated mining‐influenced increases for several elements in multiple media. In most media, concentrations of several elements including Ni were correlated positively. All water‐column dissolved concentrations were below protective levels, but fine‐sediment concentrations of Ni approached or exceeded threshold‐effect concentrations in several streams. Further study is warranted for several elements (Cd, Ni, and Zn in biofilms, and V in macroinvertebrates) that approached or exceeded previously established dietary‐risk levels. Environ Toxicol Chem 2023;42:2651–2665. © 2023 SETAC.
Future prospects in mRNA vaccine development

The recent advancements in messenger ribonucleic acid (mRNA) vaccine development have vastly enhanced their use as alternatives to conventional vaccines in the prevention of various infectious diseases and treatment of several types of cancers. This is mainly due to their remarkable ability to stimulate specific immune responses with minimal clinical side effects. This review gives a detailed overview of mRNA vaccines currently in use or at various stages of development, the recent advancements in mRNA vaccine development, and the challenges encountered in their development. Future perspectives on this technology are also discussed.
Interpreting Paleoenvironments, Subsidence History and Sea-Level Changes of Passive Margins From Seismic and Biostratigraphy

Interaction between basement subsidence, eustatic sea-level changes, and varying sediment supply shapes the sediment accumulations along passive continental margins. Detailed analysis of the sediments with seismic stratigraphy and well data permits quantification of the subsidence history and reconstruction of paleoenvironments and sea-level changes through time. Geohistory analysis provides a quantitative analysis of basin subsidence using geologic time-depth diagrams to visualize the total basin subsidence. Long-term eustatic changes are a significant component of the thermo-tectonic subsidence curve. These changes can be quantified by measuring dpearture from an established set of calculated subsidence curves. Short-term, rapid changes of sea level can be demonstrated from seismic and well data. The stratigraphic resolution of these changes rarely allows exact quantification of their magnitude, but a minimum rate of change of sea level can often be determined. Applications of these procedures are demonstrated with an example from northwest Africa.
Leisure time and occupational physical activity, overall and cardiovascular mortality: a 24-year follow-up in the OPERA study

Abstract Background In earlier studies, the health benefits of physical activity have only been related to leisure time physical activity (LTPA). High occupational physical activity (OPA) might even be harmful. The current physical activity recommendations do not separate the OPA and LTPA. We investigated the effect of LTPA and OPA on cardiovascular morbidity and mortality during long-term follow-up. We also examined how heavy work affects the benefits of leisure time exercise. Material and methods The study was part of the OPERA study and the baseline examinations were conducted between the years 1991 and 1993. The Follow-up of events continued until the end of the year 2020. Study subjects (n = 1044) were divided into four groups according to their LTPA (“no exercise”, “irregular”, “regular” and “heavy regular”) and into three groups according to their OPA (“no activity”, “mild” and “heavy”). The amount of exercise was self-reported and the exercise status was defined at the beginning of the study. Study subjects were followed up for their overall mortality (26 years), fatal and non-fatal CVD events (24 and 20 years) and heart failure (20 years). The survival analysis was performed using Kaplan–Meier curves and Cox-proportional hazard models. Results “Heavy” OPA group subjects belonging to the “irregular” (less than 1–2 times 30 min exercise per week) LTPA group experienced the lowest overall mortality compared to other LTPA groups. Also, overall mortality was increased in the “mild” (p = 0.002) and CVD mortality in the” heavy” (p = 0.005) OPA group compared to “no activity”. The incidence of heart failure was increased in the “no exercise” LTPA compared to the “heavy regular” (p = 0.015) group. Conclusions Study subjects who were in physically demanding occupations (heavy OPA) seemed to benefit from less LTPA than WHO currently recommends. Thus we suggest targeting different LTPA recommendations to different OPA groups.