Miracle-dz/UniFAR-FTUs huggingface dataset:
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep

Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent the progression of clinical disease in most animals but do not fully protect them against infection and induce the formation of an injection site granuloma. The precise mechanisms that operate in response to vaccination and granuloma development, as well as the effect that adjuvants could trigger, have not been fully investigated. Therefore, this study aimed to investigate the injection site granulomas induced by two inactivated paratuberculosis vaccines, which differ in the adjuvant employed. Two groups of 45-day-old lambs were immunized with two commercially available vaccines—one (n = 4) with Gudair® and the other (n = 4) with Silirum®. A third group (n = 4) was not vaccinated and served as control. The peripheral humoral response was assessed throughout the study by a commercial anti-Mycobacterium avium subspecies paratuberculosis (Map) antibody indirect ELISA, and the cellular immune response was assessed similarly by the IFN-γ release and comparative intradermal tests. The injection site granulomas were measured during the experiment and sampled at 75 days post-vaccination (dpv) when the animals were euthanized. The tissue damage, antigen and adjuvant distribution, and the presence and amount of immune cells were then determined and assessed by immunohistochemical methods. Antibodies against Map antigens; a general macrophage marker (Iba1), M1 (iNOS), and M2 (CD204) macrophages; T (CD3), B (CD20), and γδ T lymphocytes, proteins MHC-II and NRAMP1, and cytokines IL-4, IL-10, TNF, and IFN-γ were employed. Silirum® elicited a stronger peripheral cellular immune response than Gudair®, while the latter induced larger granulomas and more tissue damage at the site of injection. Additionally, adjuvant and Map antigen distribution throughout the granulomatous inflammatory infiltrate, as well as the NRAMP1 cell expression, which is linked to antigen phagocytosis, were highly irregular. In Silirum® induced granulomas, a higher number of MHC-II and TNF-expressing cells and a lower number of M2 macrophages suggested an improved antigen presentation, which could be due to the better antigen distribution and reduced tissue damage induced by this vaccine.
What is known about the health effects of non-steroidal anti-inflammatory drug (NSAID) use in marathon and ultraendurance running: a scoping review

This systematic scoping review aimed to understand the extent and scope of evidence on the health risks of non-steroidal anti-inflammatory drug (NSAID) use in marathon and ultraendurance running. NSAIDs are commonly consumed by runners to combat pain and inflammation; however, the health risks of consuming these drugs during marathon and ultrarunning events are currently not fully understood. Four databases (Cochrane Library, PubMed, MEDLINE and SPORTDiscus) were searched to identify articles focusing on running events of 26.2 miles or further, and they must have reported on the health risks of NSAID use. There was no restriction on the study design or the date of publication. Thirty studies were ultimately included: 4 randomised controlled trials, 1 cross-sectional study, 11 retrospective reviews, 4 case reports, 1 non-randomised control trial, and 9 prospective observational studies. The literature showed that potential health concerns of NSAID use could be split into five categories: electrolyte balance and hyponatraemia; acute kidney injury (AKI); gastrointestinal disturbances; oxidative stress, inflammation and muscle damage; other medical concerns. None of these sections had clear statistically significant links with NSAID use in ultraendurance running. However, potential links were shown, especially in AKI and electrolyte balance. This review suggests there is very limited evidence to show that NSAIDs have a negative impact on the health of ultrarunning athletes. Indications from a few non-randomised studies of a possible effect on kidney function need exploring with more high-quality research.
Density Functional Theory Studies on the Chemical Reactivity of Allyl Mercaptan and Its Derivatives

On the basis of density functional theory (DFT) at the B3LYP/cc-pVQZ level with the C-PCM solvation model, a comparative analysis of the reactivity of the garlic metabolites 2-propenesulfenic acid (PSA) and allyl mercaptan (AM, 2-propene-1-thiol) was performed. In particular, the thermodynamic descriptors (BDE, PA, ETE, AIP, PDE, and Gacidity) and global descriptors of chemical activity (ionization potential (IP), electron affinity (EA), chemical potential (μ), absolute electronegativity (χ), molecular hardness (η) and softness (S), electrophilicity index (ω), electro-donating (ω−) and electro-accepting (ω+) powers, and Ra and Rd indexes) were determined. The calculations revealed that PSA is more reactive than AM, but the latter may play a crucial role in the deactivation of free radicals due to its greater chemical stability and longer lifetime. The presence of a double bond in AM enables its polymerization, preserving the antiradical activity of the S-H group. This activity can be amplified by aryl-substituent-containing hydroxyl groups. The results of the calculations for the simplest phenol–AM derivative indicate that both the O-H and S-H moieties show greater antiradical activity in a vacuum and aqueous medium than the parent molecules. The results obtained prove that AM and its derivatives can be used not only as flavoring food additives but also as potent radical scavengers, protecting food, supplements, cosmetics, and drug ingredients from physicochemical decomposition caused by exogenous radicals.
Compact Single-Stage Micro-Inverter with Advanced Control Schemes for Photovoltaic Systems

This paper proposes a grid-connected single-stage micro-inverter with low cost, small size, and high efficiency to drive a 320 W class photovoltaic panel. This micro-inverter has a new and advanced topology that consists of an interleaved boost converter, a full-bridge converter, and a voltage doubler. Variable switching frequency and advanced burst control schemes were devised and implemented. A 320 W prototype micro-inverter was very compact and slim with 60-mm width, 310-mm length, and 30-mm height. In evaluations, the proposed micro-inverter achieved CEC weighted efficiency of 95.55%, MPPT efficiency >95% over the entire load range, and THD 2.65% at the rated power. The proposed micro-inverter is well suited for photovoltaic micro-inverter applications that require low cost, small size, high efficiency, and low noise.
Event-Triggered Synchronization Control of Uncertain Neutral-Type Neural Networks With Time-Varying Delays and Actuator Saturation

A class of event-triggered synchronization control of uncertain neutral-type neural networks (NTNNs) with time-varying delays and actuator saturation is discussed in this paper. We propose a novel NTNNs model by combining it with an event-triggered mechanism (ETM), actuator saturation and uncertainty. The ETM is introduced to determine whether the sampled signals should be transmitted to controller or not and actuator saturation is considered due to the complex network environment in reality. With the aid of proper Lyapunov-Krasovskii functional (LKF), some sufficient conditions for asymptotic stability of synchronization error system are derived and the gain of the controller is obtained by using linear matrix inequality (LMI) and free-weight matrix (FWM) methods. Finally, we use Euler-Maruyama numerical mechanism to simulate the drive-response systems and the error system, which shows the good convergence performance of our synchronization model. Two numerical examples in the end of this paper demonstrate the effectiveness of the proposed method.
Effect of melt/mantle interactions on MORB chemistry at the easternmost Southwest Indian Ridge (61°–67°E)

The easternmost part of the Southwest Indian Ridge (61°–67°E) is an end‐member of the global ridge system in terms of very low magma supply. As such, it is a good laboratory to investigate the effect of melt/mantle interactions on the composition of erupted basalts: for a given volume of erupted basaltic melt, the volume of reacted mantle is potentially greater than at more magmatically robust ridges. We analyzed major, trace element and isotopic compositions in three groups of rocks: plagioclase‐bearing ultramafic and gabbroic rocks dredged in nearly amagmatic spreading corridors; basalts from the sparse volcanic cover of these corridors (“ultramafic seafloor basalts”); and basalts dredged from the intervening, more volcanically active domains (“volcanic seafloor basalts”). Ultramafic seafloor basalts have significantly lower CaO and Al2O3 contents at a given MgO than most volcanic seafloor basalts. We propose that both types of basalts are derived from similar parental melts, but that the ultramafic seafloor basalts are more affected by reactions between these parent melts and the mantle rocks in the lithosphere below the ridge. We infer that these reactions occur in the walls of conduits that allow the aggregated melts extracted from the melting mantle to rise through the axial lithosphere and to the eruption sites. The principal effect of these reactions is to enrich the asthenospheric melts in MgO through olivine dissolution. This effect is not expected to be as noticeable, but could still play a role in basalt petrogenesis at more magmatic regions of the global slow‐spreading MOR system.
Changing Sleep Architecture through Motor Learning: Influences of a Trampoline Session on REM Sleep Parameters

Previous research has shown that learning procedural tasks enhances REM sleep the following night. Here, we investigate whether complex motor learning affects sleep architecture. An experiment in which twenty-two subjects either learned a motor task (trampolining) or engaged in a control task (ergometer) was carried out in a balanced within-group design. After an initial laboratory adaptation night, two experimental nights were consecutive. The results indicate that learning a motor task had an effect on REM sleep parameters and, therefore, support the hypothesis that learning a procedural skill is related to an increase in REM sleep parameters. However, the statistical effect on REM sleep is smaller than found in previous studies. One might speculate that the motor learning was not intense enough compared to other studies. For sports practice, the results suggest that REM sleep, which is particularly rich in the morning, plays an important role in motor memory consolidation. Thus, this phase should not be interrupted after complex motor skill learning sessions. In future studies, other motor tasks should be applied.
THE PROPENSITY OF BUILDING INFORMATION MODELLING AND INTEGRATED PROJECT DELIVERY IN BUILDING CONSTRUCTION PROJECT

The Building Information Modelling (BIM) implementation is to create a centralised knowledge sharing resource that contains all the necessary design and operational information about the project. BIM is a collaborative approach and integrated project delivery to design and delivery embraced by various construction teams in the construction projects. In the context of the most suitable project delivery to be used, with regards to the use of BIM, the Integrated Project Delivery (IPD) approach is highlighted as the best method which correlates building procurement of the design-to-construction business process through literature review analysis. IPD is developed as project delivery system that integrates people in effective collaborative approach between the key players in the project. IPD is going to be particularly beneficial for BIM because the contracts relation between the contractor, designers, consultants, constructors, sub-contractors and suppliers were binded and expressly determined at earlier stage. This relationship between these two subjects will helped the project team to achieve the project goals in terms of timely schedule, life cycle costs, quality and sustainability in construction project. Therefore, this study investigates on the propensity of the best methodology of project delivery system which can correlate with procurement adoption for projects adapt BIM concept. This is a descriptive study and the method used is based on review of the literature in relation to BIM and IPD. The analysis revealed that propensity of adopting IPD project delivery and the adoption of BIM are based on these factors: improve the communication, closer collaboration; liability and legal relationships are specifically determined, the best quality outcome; and lastly with reductions in design and construction period, it provides a cost effectiveness and a saving in overall construction period.
The Effect of Severe Drought on the Evolution of Urban and Manure Wastes in an Agricultural Soil in Mediterranean Ecosystems

In semi‐arid Mediterranean soils, water availability is the most limiting factor, negatively affecting the organic matter (OM) degradation. The aim of this work is to study under controlled laboratory conditions how three sources of OM [municipal solid waste (MSW), sheep manure (SM) and cow manure (CM)] behave when they are applied to an agricultural soil subjected to a severe year‐long drought. In order to apply the same concentration of OM to the soil (16·92 Mg OM ha−1), 2 kg of soil was mixed with 30, 67·41 and 55·25 Mg ha−1 (dry matter) of MSW, CM and SM, respectively. Two levels of irrigation were employed: (i) watered soils and (ii) non‐watered soils. Soil's chemical properties [water soluble carbon (WSC), humic acids, fulvic acids and protein mass distribution], biological properties (soil microbial biomass carbon and o‐diphenoloxidase activity) and solid‐state 13C cross‐polarisation magic angle spinning nuclear magnetic resonance spectroscopy were determined. In watered soils, the soil microbial biomass carbon was higher in the SM than in CM and MSW treatments (9·9% and 23·1%, respectively). The WSC was significantly higher in SM than in CM (55·7%) and MSW (78·7%) treatments. A decrease in the content of O‐alkyl C and an increase in alkyl C, aromatic C and carboxyl C were observed. In non‐watered soils, the biochemical properties and alkyl C and alkyl/O‐alkyl ratio decreased, whereas WSC content and O‐alkyl C increased. These results indicated that the evolution of OM and the activity of the microbial community in non‐watered soils were very different to those in the watered soils. Copyright © 2016 John Wiley & Sons, Ltd.
9. LACK OF ISOTOPIC FRACTIONATION OF DELTA15N OF ORGANIC MATTER DURING LONG-TERM DIAGENESIS IN MARINE SEDIMENTS, ODP LEG 202, SITES 1234 AND 1235

The isotopic composition of nitrogen in pore water ammonium and in sedimentary organic matter (Norg) was measured at Sites 1234 and 1235 in order to evaluate the impact of long-term (>100 k.y.) diagenesis on δ15N of preserved organic matter. At both sites, the average δ15N of pore water ammonium and Norg are within 0.2‰ to 0.4‰. The small difference is less than the analytical uncertainty, indicating that no significant isotopic fractionation is associated with decomposition of organic matter in these sediments. A mass balance for nitrogen was also computed, indicating that ~20% of the organic matter flux buried below 1.45 meters composite depth (mcd) is degraded between this depth and 40 mcd (Site 1235) to 60 mcd (Site 1234) depth. Two factors determine the absence of isotopic fractionation in these sediments: 1. A high degree of organic matter preservation due to rapid sediment accumulation rates at both sites. 2. The dominance of a marine component in the sedimentary organic matter (with only a small fraction contributed by a terrestrial component). 1Prokopenko, M.G. , Hammond, D.E. , and Stott, L. , 2006. Lack of isotopic fractionation of δ15N of organic matter during long-term diagenesis in marine sediments, ODP Leg 202, Sites 1234 and 1235. In Tiedemann, R. , Mix, A.C. , Richter, C. , and Ruddiman, W.F. (Eds.), Proc. ODP, Sci. Results, 202: College Station, TX (Ocean Drilling Program), 1–22. doi:10.2973/ odp.proc.sr.202. 207.2006 2Department of Earth Sciences, University of Southern California, 3651 Trousedale Parkway, Los Angeles CA 90089-0740, USA. 3Present address: Department of Geosciences, Guyot Hall, Washington Road, Princeton University, Princeton NJ 08544, USA. mprokope@princeton.edu Initial receipt: 15 October 2004 Acceptance: 28 April 2006 Web publication: 24 October 2006 Ms 202SR-207 M.G. PROKOPENKO ET AL. LACK OF ISOTOPIC FRACTIONATION OF δ15N 2
Evaporation over the Baltic Sea as an example of a semi-enclosed sea

Evaporation is a major term in the energy and water cycle of the Baltic Sea. Wide areas of a semienclosed sea like the Baltic Sea belong to the coastal zone, which is a transition zone from the different roughnesses and thermal properties of the open sea and the land surfaces. This causes that wind speeds in coastal areas are generally lower than over the open sea for the same geostrophic wind speeds somewhat dependent on the wind direction relative to the coast. Furthermore the evaporation is hampered by ice, which covers parts of the Baltic Sea during winter time and is more prevalent near the coast. Sea ice and the influence of the coast on the wind speed reduce evaporation by up to about 7 to 8% each. The resulting evaporation is of the same order as previous estimates and shows similar spatial and temporal patterns, but uncertainties remain. It is shown that these depend mainly on the boundary layer parameterization used to calculate the evaporation.
Hydrocarbon Habitat of the Rockall Trough, Northeast Atlantic Margin

The Rockall Trough is a rift basin located on the North Atlantic passive margin, west of the UK and Ireland. It is a frontier basin with very limited well control. Most exploration wells are confined to the inboard basin areas and near igneous complexes. None of the wells drilled to date have penetrated source rocks, nor have any economically significant hydrocarbon discoveries been made. Consequently, explorers face considerable uncertainties in understanding key elements of the hydrocarbon system, such as the distribution of potential source rocks, their thermal maturity levels, and the timing of oil and gas yields. Since the UK government's 17th licensing round announcement in November 1996, the area has been the subject of numerous studies for both industry and academia. This particular study was undertaken to: • determine whether or not an effective oil-prone source rock is present, • characterize the nature of any hydrocarbon seepage in terms of source rock organic facies, thermal maturity, geological age, and degree of post-generative alteration, • evaluate migration pathways, • determine the timing of oil and gas yield, by way of one-dimensional basin modeling, and apply the results from seafloor geochemistry analyses to audit the modeled hydrocarbon yield composition and yield timing, and • determine the sensitivity of thermal maturity and hydrocarbon yield predictions to uncertainties in the sparse constraining data.
Improved Chambadal Model with New Optimization Results

This paper presents a continuation of the Chambadal model optimization of the irreversible Carnot engine. We retrieved the results presented in the Special Issue “Carnot Cycle and Heat Engine Fundamentals and Applications II” and enriched them with new contributions that allowed comparing two points of view: (1) the now classical one, centered on entropy production in the four processes of the cycle, which introduces the action of entropy production, with several sequential optimizations; (2) the new one that is relative to an energy degradation approach. The same démarche of sequential optimization was used, but the results were slightly different. We estimate that the second approach is more representative of physics by emphasizing the energy conservation and the existence on an upper and a lower bound in the mechanical energy and power output of the engine.
Extracellular histones are clinically associated with primary graft dysfunction in human liver transplantation

Extracellular histones have been involved in numerous inflammatory conditions such as ischemia/reperfusion (I/R) injury, trauma, and infection. There is growing evidence of I/R injury associated with primary graft dysfunction (PGD) following organ transplantation. Here we investigated whether extracellular histones are clinically involved with PGD in human liver transplantation. In total 58 patients undergoing liver transplantation were studied. We collected blood samples from the recipients before and serially after transplantation (24 h, 72 h). We measured extracellular histones, myeloperoxidase (MPO), S100A8/A9, and multiple inflammatory cytokines. Additionally, we exposed human L02 hepatocytes or U937 monocytic cells to the recipient's sera overnight, and assessed cellular viability and cytokine production respectively. Lastly, we assessed the effect of histone-targeted interventions by administration of heparin or an anti-histone antibody. It showed that extracellular histones increased immediately after transplantation, peaked within 24 hours and remained at high levels up to 72 hours (all p < 0.01). Notably, extracellular histone levels were significantly higher in recipients with PGD (n = 9) than recipients without PGD (n = 49, p = 0.004). Extracellular histones correlated positively with MPO, S100A8/A9 and most detected cytokines. Ex vivo analysis demonstrated that the patients' sera after graft markedly induced L02 cell death and caused profound cytokine production in cultured U937 cells, which could be abrogated by heparin or an anti-histone antibody. Collectively, extracellular histones were increased significantly after liver transplantation, which may contribute to the occurrence of PGD through direct cytotoxicity and enhancement of systemic inflammation. Targeting extracellular histones may provide a promising approach for preventing PGD or other complications in clinical practice.
Broadband optical properties of biomass‐burning aerosol and identification of brown carbon chromophores

The radiative effects of biomass‐burning aerosols on regional and global scales can be substantial. Accurate modeling of the radiative effects of smoke aerosols requires wavelength‐dependent measurements and parameterizations of their optical properties in the UV and visible spectral ranges along with improved description of their chemical composition. To address this issue, we used a recently developed approach to retrieve the time‐ and spectral‐dependent optical properties of ambient biomass‐burning aerosols from 300 to 650 nm wavelengths during a regional nighttime bonfire festival in Israel. During the biomass burning event, the overall absorption at 400 nm increased by about 2 orders of magnitude, changing the single scattering albedo from a background level of 0.95 to 0.7. Based on the new retrieval method, we provide parameterizations of the wavelength‐dependent effective complex refractive index from 350 to 650 nm for freshly emitted and slightly aged biomass‐burning aerosols. In addition, PM2.5 filter samples were collected for detailed offline chemical analysis of the water‐soluble organics that contribute to light absorption. Nitroaromatics were identified as major organic species responsible for the increased absorption at 400 to 500 nm. Typical chromophores include 4‐nitrocatechol, 4‐nitrophenol, nitrosyringol, and nitroguaiacol; oxidation‐nitration products of methoxyphenols; and known products of lignin pyrolysis. Our findings emphasize the importance of both primary and secondary organic aerosols from biomass burning in absorption of solar radiation and in effective radiative forcing.