Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of the neuroprotective efficacy of the gramine derivative ITH12657 against NMDA-induced excitotoxicity in the rat retina

Purpose The aim of this study was to investigate, the neuroprotective effects of a new Gramine derivative named: ITH12657, in a model of retinal excitotoxicity induced by intravitreal injection of NMDA. Methods Adult Sprague Dawley rats received an intravitreal injection of 100 mM NMDA in their left eye and were treated daily with subcutaneous injections of ITH12657 or vehicle. The best dose–response, therapeutic window study, and optimal treatment duration of ITH12657 were studied. Based on the best survival of Brn3a + RGCs obtained from the above-mentioned studies, the protective effects of ITH12657 were studied in vivo (retinal thickness and full-field Electroretinography), and ex vivo by quantifying the surviving population of Brn3a + RGCs, αRGCs and their subtypes α-ONsRGCs, α-ONtRGCs, and α-OFFRGCs. Results Administration of 10 mg/kg ITH12657, starting 12 h before NMDA injection and dispensed for 3 days, resulted in the best significant protection of Brn3a + RGCs against NMDA-induced excitotoxicity. In vivo, ITH12657-treated rats showed significant preservation of retinal thickness and functional protection against NMDA-induced retinal excitotoxicity. Ex vivo results showed that ITH12657 afforded a significant protection against NMDA-induced excitotoxicity for the populations of Brn3a + RGC, αRGC, and αONs-RGC, but not for the population of αOFF-RGC, while the population of α-ONtRGC was fully resistant to NMDA-induced excitotoxicity. Conclusion Subcutaneous administration of ITH12657 at 10 mg/kg, initiated 12 h before NMDA-induced retinal injury and continued for 3 days, resulted in the best protection of Brn3a + RGCs, αRGC, and αONs-RGC against excitotoxicity-induced RGC death. The population of αOFF-RGCs was extremely sensitive while α-ONtRGCs were fully resistant to NMDA-induced excitotoxicity.
Resultados del test intradérmico en perros con dermatitis atópica: estudio retrospectivo de 176 casos en Andalucía

espanolEn perros con dermatitis atopica, la identificacion de los alergenos sensibilizantes es importante para evitar su contacto e instaurar una inmunoterapia alergeno-especifica. Los alergenos potencialmente sensibilizantes son numerosos y varian segun la region geografica donde el animal resida. Por este motivo, y con independencia del test que elijamos, es importante conocer los alergenos con mayor prevalencia de reacciones positivas en cada region geografica. En este estudio retrospectivo analizamos los resultados del test intradermico realizado en 176 perros con dermatitis atopica residentes en Andalucia (Cordoba, Sevilla y Jaen, principalmente). En total se ensayaron 52 alergenos diferentes. Los alergenos no estacionales, como los acaros, alcanzaron los porcentajes mas elevados de positivos, especialmente D. farinae (48,30 %) seguido de T. putrescientiae (35,96 %), Acarus siro (32,58 %), D. pteronyssinus (23,30 %) y L. destructor (22,47 %). Entre los alergenos estacionales, los polenes de olivo (27,27 %), Artemisia vulgaris (21,02 %) y gramineas como Phleum pratense (18,18 %), Hordeum vulgare (17,05 %), Cynodon dactylon (16,48 %) y Lolium perenne (15,34 %) fueron los mas prevalentes. Estos resultados pueden ser utiles para la seleccion de un panel regionalizado de alergenos a incluir en las pruebas de alergia, tanto serologicas como intradermicas, realizadas a perros con dermatitis atopica que vivan en esta zona geografica o en otras zonas de Espana con un clima similar. EnglishThe identification of sensitizing allergens in dogs with atopic dermatitis is important in order to avoid the exposure and to give an allergen-specific immunotherapy. There are many potential sensitizing allergens and they vary depending on the geographic area where the dog lives. Because of this, and independently of the method employed, it is important to identify what allergens have a higher positive prevalence in each geographic area. In the present retrospective study, intradermal tests from 176 dogs with atopic dermatitis living in a region of dry continental Spain (Andalusia -mainly from the provinces of Cordova, Seville and Jaen) were analyzed. A total of 52 allergens were assayed. Non-seasonal allergens as mites reached the highest percentage of positive reactions, especially D. farinae (48.30 %), followed by T. putrescientiae (35.96 %), Acarus siro (32.58 %), D. pteronyssinus (23.30 %) and L. destructor (22.47 %). Concerning seasonal allergens, pollens of olive tree (27.27 %), Artemisia vulgaris (21.02 %) as well as grasses like Phleum pratense (18.18 %), Hordeum vulgare (17.05 %), Cynodon dactylon (16.48 %) and Lolium perenne (15.34 %) were the most prevalent. The obtained results may be useful to select a panel of allergens to be included in intradermal as well as serological allergy tests, designed for dogs with atopic dermatitis living in the same area or in others areas of Spain with a similar climate.
Visualizing the invisible: novel approaches to visualizing bacterial proteins and host-pathogen interactions

Host-pathogen interactions play a critical role in infectious diseases, and understanding the underlying mechanisms is vital for developing effective therapeutic strategies. The visualization and characterization of bacterial proteins within host cells is key to unraveling the dynamics of these interactions. Various protein labeling strategies have emerged as powerful tools for studying host-pathogen interactions, enabling the tracking, localization, and functional analysis of bacterial proteins in real-time. However, the labeling and localization of Salmonella secreted type III secretion system (T3SS) effectors in host cells poses technical challenges. Conventional methods disrupt effector stoichiometry and often result in non-specific staining. Bulky fluorescent protein fusions interfere with effector secretion, while other tagging systems such as 4Cys-FLaSH/Split-GFP suffer from low labeling specificity and a poor signal-to-noise ratio. Recent advances in state-of-the-art techniques have augmented the existing toolkit for monitoring the translocation and dynamics of bacterial effectors. This comprehensive review delves into the bacterial protein labeling strategies and their application in imaging host-pathogen interactions. Lastly, we explore the obstacles faced and potential pathways forward in the realm of protein labeling strategies for visualizing interactions between hosts and pathogens.
The ants of the genus Rhopalothrix Mayr, 1870 (Hymenoptera, Formicidae, Myrmicinae) in Colombia

﻿Abstract The ants of the genus Rhopalothrix are diverse in the Neotropical region, with 14 of the 16 described species. Based on museum material and recent fieldwork, Rhopalothrix ants in Colombia were reviewed. Morphological analysis of the workers allowed delimitation of six species, including two new species, Rhopalothrixmandibularis Guerrero & Grajales, sp. nov. and Rhopalothrixmariaemirae Tocora, Fiorentino & Fernández, sp. nov. A new combination Rhopalothrixamaticomb. nov. is proposed for Eurhopalothrixamati. A worker-based taxonomic key, high-definition images of the workers, and a distribution map of all Rhopalothrix species present in Colombia are provided.
Exploring global and local processes underlying alterations in resting-state functional connectivity and dynamics in schizophrenia

Introduction We examined changes in large-scale functional connectivity and temporal dynamics and their underlying mechanisms in schizophrenia (ScZ) through measurements of resting-state functional magnetic resonance imaging (rs-fMRI) data and computational modelling. Methods The rs-fMRI measurements from patients with chronic ScZ (n=38) and matched healthy controls (n=43), were obtained through the public schizConnect repository. Computational models were constructed based on diffusion-weighted MRI scans and fit to the experimental rs-fMRI data. Results We found decreased large-scale functional connectivity across sensory and association areas and for all functional subnetworks for the ScZ group. Additionally global synchrony was reduced in patients while metastability was unaltered. Perturbations of the computational model revealed that decreased global coupling and increased background noise levels both explained the experimentally found deficits better than local changes to the GABAergic or glutamatergic system. Discussion The current study suggests that large-scale alterations in ScZ are more likely the result of global rather than local network changes.
Structure of the vertebral column in Eusthenopteron foordi Whiteaves

X-ray photographs of part of the vertebral column of Eusthenopteron foordi Whiteaves show that each vertebral segment consists of (1) a centrum made up of two thin lateral concentric half rings; (2) large paired neural~arches and neural spines; and (3) small paired pleurocentra at the bases of the neural arches. There is no indication of more than one ring centrum associated with a neural arch, the intercentral space presumably having been cartilaginous. The embolomerous condition of early amphibians may have arisen through ossification of the cartilaginous intercentral space or by down growth of the pleurocentra to form a second ring, or by a combination of these two processes. T IS A surprising fact that, while Gadow and many other authors have studied the problem of the origin of vertebrae from embryological material in recent amphibians and reptiles, not much attention has been paid to the structure of the vertebrae of Palaeozoic crossopterygians. Although it is generally assumed that the embolomerous type of vertebrae found in primitive amphibians was derived from some crossopterygian ancestor, there is remarkably little direct evidence as to whether this two-ringed condition was present before amphibians
UAV Application for Process Control of the Reclamation Project

ABSTRACT Kim, J.O. and Lee, J.K, 2017. UAV application for process control of the reclamation project. In: Lee, J.L. ; Griffiths, T. ; Lotan, A. ; Suh, K. -S. , and Lee, J. (eds.), The 2nd International Water Safety Symposium. Journal of Coastal Research, Special Issue No. 79, pp. 309–313. Coconut Creek (Florida), ISSN 0749-0208. It is essential to acquire an accurate topographic information to efficiently operate a reclamation project. However, the measurement of large-size reclaimed land using terrestrial survey methods, such as total station and GPS requires huge number of efforts in terms of time and labor spending. Moreover, the coastal surveying that should be remote and hard to reach is extremely dangerous and sometime impossible. Therefore, in this investigation, UAV (Unammaned Aerail Vehicle) which has been actively considering in the field of spatial information currently, was applied for the construction management of reclamation project. In this paper, the feature matching and SfM (Structure from Motion) algorithm were used as image correction and matching technology. The UAV images were used to create three dimensional point clouds. The points clouds are transformed and aligned to global coordinate by using 21 GCPs (Ground Control Points) which are previously measured by terrestrial surveying. The aligned point clouds were eventually used to generate a DEM (Digital Elevation Model) and ortho-imagery. This study also assessed the positioning accuracy of the results by comparing with terrestrial surveying results using total stations at 426 check points on the study area. The RMSE (Root Mean Square Error) of positioning differences between the coordinates of UAV photogrammetry and terrestrial surveying was within tolerance, 4.8 cm, except for the results at non-ground points. This paper, consequently, presented an availability of the UAV for the photogrammetry, so that it can be fully expected the UAV photogrammetry could be applied in many fields included construction application.
Bottomland Hardwoods of the Mississippi Alluvial Valley: Characteristics and Management of Natural Function, Structure, and Composition

913. Bottomland Hardwoods of the Mississippi Alluvial Valley: Characteristics and Management of Natural Function, Structure, and Composition. Hamel, Paul B. and Foti, Thomas L. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station; GTR-SRS-042, 2001. 109 p. NAL Call #: aSD143 . G46 no. 42 http://www.srs. fs.usda. gov/pubs/2474 Descriptors: bottomland hardwoods/ forest history/ mammals/ Mississippi Alluvial Valley/ old growth/ songbirds/ wetland restoration Abstract: A symposium entitled "Bottomland hardwoods of the Mississippi Alluvial Valley: characteristics and management of natural function, structure, and composition" convened on October 28, 1995, as part of the Natural Areas Conference, October 25-28, 1995, In Fayetteville, AR. The symposium's goal was to provide informatibn that managers need to begin restoring the composition, structure, and function of off rest ecosystems in the Mississippi Alluvial Valley.Included in the proceedings from that symposium are 8 of 13 presentations. These peer-reviewed contributions address historical conditions of forests in the Mississippi Alluvial Valley (two papers), historical changes that are reflected in today's forests (one paper), the effect of historic and prehistoric rainfall patterns (one paper), forest fauna in the region (two papers), the effect of herbivory on forest vegetation (one paper), and management of bottomland hardwood forests for multiple outputs (one paper). A ninth paper, concerning characteristics of old-growth forests, is a posthumous submission authored by Dr. James T. Tanner; and the tenth paper was published in another venue. The intended audience of these proceedings includes managers of private, State, and Federal lands, as well as land management planners from a range of jurisdictions.
Metacognitive beliefs of efficacy about daily life situations and use of cognitive strategies in amnestic mild cognitive impairment: a cross-sectional study

Metacognition, the ability to monitor and regulate cognitive processes, is essential for individuals with Mild Cognitive Impairment (MCI) to accurately identify their deficits and effectively manage them. However, previous studies primarily focused on memory awareness in MCI, neglecting other domains affected in daily life. This study aimed to investigate how individuals with MCI perceive their abilities to handle various cognitively challenging situations representing real-life scenarios and their use of compensatory strategies. Thus 100 participants were recruited, including 50 with amnestic MCI with multiple deficits (aMCI) and 50 cognitively healthy controls (HC) matched in age and education. Participants completed three metacognitive scales assessing self-perceived efficacy in everyday life scenarios and one scale evaluating use of cognitive strategies. Results indicated that aMCI participants reported significantly lower self-efficacy in memory and divided-shifted attention scenarios compared to HC. Surprisingly, no significant group differences were found in the self-reports about the use of cognitive strategies. This suggests a potential gap in understanding or applying effective strategies for compensating cognitive deficits. These findings emphasize the importance of cognitive training programs targeting metacognitive knowledge enhancement and practical use of cognitive strategies that could enhance the quality of life for individuals with MCI.
AREA DE DEMENCIA TEST DIAGNOSTICO El "Mini - Mental State Examination" en español. Normas para Buenos Aires

Summary Background: The Mini-Mental State Examination (MMSE) is one of the most widely used tools for the screening of cognitive impairment. The informal use in our country of non validated and poorly translated versions of the MMSE to Latin American Spanish language is widespread and presumably this fact further increases some of the uncertainties linked to this test. Objective: The purpose of this study was to collect normative information in Buenos Aires and to evaluate sex, age. and education effects on the MMSE. Methods: 634 healthy Spanish-speaking subjects participated in this study. The age range was from 30 to 94 years. Education was 11.7 (± 4.1 ) years. All of them underwent a comprehensive Neuropsychological Battery and the Buenos Aires version of the Mini Mental State Examination. The sample was stratified in five groups: less than 45 years old, and every 10 years, until 75 years old and more with three different levels of education: 4-7; 8-12; >13 years. Our results correlate significantly with age and education. Mean performance scores are presented for each group to use in an Argentine population.
Beneath the surface: a case report on nonencapsulated Streptococcus pneumoniae-associated invasive disease in an immunocompromised patient

Background. Streptococcus pneumoniae, a prominent human pathogen linked to various systemic diseases, includes non-typeable pneumococci marked by the absence of a detectable capsule. However, the majority of invasive infections are attributed to encapsulated strains. This case report details the first documented instance of invasive disease caused by non-typeable S. pneumoniae in Argentina since 2017. Case Presentation. A 19-year-old woman presented with haemorrhagic injuries attributed to chronic oral mucosa irritation. Subsequent hospitalization revealed bone marrow aplasia, leading to antibiotic, antifungal, antiviral, and immunosuppressive treatments, culminating in her discharge. Two weeks later, she was readmitted with sepsis related to a respiratory focus, exhibiting a negative COVID-PCR test. After ten days, ICU admission revealed additional infections: positive COVID-PCR test, fungal sinusitis, and S. pneumoniae bacteremia. Targeted treatments led to improvement, and the patient was subsequently discharged. S. pneumoniae characterization. Verification of the capsule’s absence utilized traditional methods such as the Quellung reaction, transmission electron microscopy, molecular assays, and Whole Genome Sequencing (WGS). The isolate, identified as ST18335, displayed genetic features and antibiotic resistance patterns, concordant between WGS and the agar dilution method. It demonstrated non-susceptibility to penicillin and cefotaxime, based on meningitis breakpoints, as well as meropenem and cotrimoxazole. Conclusion. This case underscores the clinical significance of non-typeable S. pneumoniae, emphasizing the necessity for a comprehensive approach to identification and characterization. The findings contribute to ongoing discussions regarding the challenges posed by non-typeable strains in vaccine development, understanding clinical impacts, and addressing antibiotic resistance. As the pneumococcal epidemiological landscape evolves, this case serves as a valuable addition to the evolving knowledge surrounding non-typeable S. pneumoniae, highlighting the continued need for surveillance and research in infectious diseases.
Concurrent Body-Coupled Powering and Communication ICs With a Single Electrode

Body-coupled powering (BCP) and body-coupled communication (BCC) utilize the human body channel as the wireless transmission medium, which shows less path loss around the body area. However, integrating both BCP and BCC requires multiple electrodes or alternating the uplink and downlink in the time domain, due to signal interferences and backflow between different paths. To address this issue, we propose a base station (BS) IC and a sensor node (SN) IC with BCP and BCC concurrency. At the BS, the adaptive self-interference cancellation (SI-C) structure suppresses the output signals that are coupled at the data receiver, enabling the concurrent uplink data recovery and downlink power delivery. At the SN, the ground domain of the uplink data path is separated from that of the downlink power/data path to suppress leakage between the paths. For regulated power supply in different ground domains, the cross-ground-domain power converter is designed with 89.1% efficiency. The ICs are implemented in a 40-nm 1P8M standard CMOS process, and BCC + BCP concurrent operations are successfully validated.
Effects of dietary energy level on antioxidant capability, immune function and rectal microbiota in late gestation donkeys

Introduction This study investigated the effects of dietary energy level on the antioxidant capability, immune function, and rectal microbiota in donkey jennets during the last 60 days of gestation. Methods Fifteen pregnant DeZhou donkeys with age of 6.0 ± 0.1 years, body weight of 292 ± 33 kg, parity of 2.7 ± 0.1 parities and similar expected date of confinement (74 ± 4 days) were randomly allocated to three groups and feed three diets: high energy (10.92 MJ/kg, H), medium energy (10.49 MJ/kg, M), and low energy (9.94 MJ/kg, L). Results and Discussion The serum activity of catalase (CAT), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in group M was significantly higher, whereas the concentrations of malondialdehyde (MDA), interleukin 1 (IL-1), IL-2, and IL-6 were lower than those recorded for groups H and L (p ≤ 0.05). The dietary energy level significantly affected rectal microbial community structure in the jennet donkeys 35 days and 7 days before the parturition (p ≤ 0.05). The abundances of norank_f_norank_o_Coriobacteriales genus was significantly higher (p ≤ 0.05) in group H, and the abundances of norank_f_norank_o_Mollicutes_RF39 and the Candidatus_Saccharimonas were higher in group L (p ≤ 0.05). The abundance of Fibrobacter in group M was significantly increased (p ≤ 0.05). The abundance of norank_f_norank_o_Coriobacteriales was positively correlated with average daily gain (ADG) and tumor necrosis factor-α (TNF-α) concentrations (p ≤ 0.05). The abundance of norank_f_norank_o_Mollicutes_RF39 was positively correlated with IL-2 and IL-6 concentrations. The abundance of Candidatus_Saccharimonas was positively correlated with CAT, T-SOD and GSH-Px activities (p ≤ 0.05). The abundance of Fibrobacter was positively correlated with CAT and T-SOD activities (p ≤ 0.05), but negatively correlated with IL-2 concentration (p ≤ 0.05). In conclusion, an appropriate dietary with an energy content of 10.49 MJ/kg for jennet donkeys during late gestation increased the prenatal antioxidant capacity, reduced inflammatory cytokines, and promoted fetal growth, and these changes were related to diet-induced changes in rectal microbiota compositions.
ALINHAMENTO ESTRATÉGICO ENTRE INDÚSTRIAS DE BENS DE CAPITAL E DE ALIMENTOS: UMA ANÁLISE COM BASE EM MILES & SNOW STRATEGIC ALIGNMENT BETWEEN CAPITAL GOODS AND FOOD PROCESSING INDUSTRIES: AN ANALYSIS USING MILES & SNOW APPROACH

The aim of this study is to know the dynamic of the strategic alignment between mechanic capital goods and food processing industries by using Miles & Snow approach. For this, the food processing was considered as client and strategic driver to its supplier, the mechanic capital goods industries. The research, considered as qualitative type, was based on in-depth interviews with managers of 6 capital goods and 6 food processing companies. Moreover, a group discussion (Focus Group) with 6 managers, being 3 of each sector was done. The data treated using the content analysis, revealed that: a) there is a relation with external context and organizational structure, internal process and manufacturing systems; b) evidences of strategic alignment according to the dynamic adaptive cycle of Miles & Snow, which it presupposes that companies develop supported by standards of strategic behavior to align with the external environment.