Miracle-dz/UniFAR-FTUs huggingface dataset:
Harmonization of criteria and terminology in fetal rat skeletal evaluations

A survey of laboratories in North American and Europe that routinely conduct fetal skeletal examinations was performed with the purpose of (1) understanding current terminology used for classifying skeletal findings in developmental toxicity (DT) studies and (2) understanding the criteria used to identify relatively common findings that sufficiently deviate from normal. The goal was to promote terminology harmonization and improve interlaboratory consistency in the criteria used to identify developmental anomalies.
Key Risk Genes Identified From the Postmortem Brain of Patients With Major Depressive Disorder and Their Potential Clinical Applications

Abstract Background Major depressive disorder (MDD) is a type of emotional dysfunction, and its pathogenesis has not been fully elucidated. Specifically, the key molecules in depression-related brain regions involved in this disease and their contributions to this disease are currently unclear. Methods GSE53987 and GSE54568 were selected from the Gene Expression Omnibus database. The data were standardized to identify the common differentially expressed genes (DEGs) in the cortex of MDD patients in the 2 datasets. The DEGs were subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The STRING database was used to build protein–protein interaction networks, and the cytoHubba plugin was used to identify hub genes. Furthermore, we selected another blood transcriptome dataset that included 161 MDD and 169 control samples to explore the changes in the screened hub genes. Mice were subjected to 4 weeks of chronic unpredictable mild stress to establish an animal model of depression, and the expression of these hub genes in tissues of the prefrontal cortex was then detected by quantitative real time polymerase chain reaction (qRT-PCR). We subsequently predicted the possible posttranscriptional regulatory networks and traditional Chinese medicine according to the hub genes using a few online databases. Results The analysis identified 147 upregulated genes and 402 downregulated genes were identified in the cortex of MDD patients compared with that of the controls. Enrichment analyses revealed that DEGs were predominantly enriched in synapse-related cell functions, linoleic acid metabolism, and other pathways. Protein–protein interaction analysis identified 20 hub genes based on the total score. The changes in KDM6B, CUX2, NAAA, PHKB, NFYA, GTF2H1, CRK, CCNG2, ACER3, and SLC4A2 in the peripheral blood of MDD patients were consistent with those in the brain. Furthermore, the prefrontal cortex of mice with depressive-like behaviors showed significantly increased Kdm6b, Aridb1, Scaf11, and Thoc2 expression and decreased Ccng2 expression compared with that of normal mice, which was consistent with the results found for the human brain. Potential therapeutic candidates, such as citron, fructus citri, leaves of Panax Notoginseng, sanchi flower, pseudoginseng, and dan-shen root, were selected via traditional Chinese medicine screening. Conclusions This study identified several novel hub genes in specific brain regions involved in the pathogenesis of MDD, which may not only deepen our understanding of depression but may also provide new ideas for its diagnosis and treatment.
Comparative analysis of bioactive compounds and antioxidant capacity of selected plants as phytogenic feed additives for poultry nutrition

Abstract The paper investigated the phytochemical content and antioxidant activity of poplar buds (Populus nigra L.), turmeric (Curcuma longa) and ginger (Zingiber officinale) extracts. Proximate composition, total polyphenols, ß-carotene, vitamin E, minerals and antioxidant capacity were tested for all selected plants. The proximate analysis of selected plants showed that poplar buds had a higher content of crude protein, fat and fiber than turmeric and ginger. The extract of poplar buds had a significantly higher content of total polyphenols (87.20 mg of gallic acid/g) and vitamin E (210.53 μg/g) than in the extract of turmeric and ginger. The polyphenol content was found to be in the order: poplar buds>turmeric> ginger. The beta carotene content was significantly higher in poplar buds (2.99 μg/g) than in turmeric (1.67 μg/g). Regarding mineral content, turmeric showed the highest content in iron and copper; ginger showed the highest content in manganese and poplar buds had the highest content in zinc. Moreover, poplar buds possessed the highest antioxidant capacity both on vitamin C and E calibration curve, evidencing the rich content in such antioxidants. Therefore, based on the valuable nutritional composition confirmed through this study, poplar buds, turmeric and ginger can be recommended as phytogenic feed additives for poultry nutrition.
Potential implications of natural antioxidants from plant to improve nutritional quality, oxidative stability and lipid degradation of egg yolk: A review

Abstract Food occupies a principal role in human life, having a major impact on health status and representing diverse characteristics that ensure valuable nutrient intake. Ensuring human nutrition has become one of the essential problems facing mankind, and quantity, quality and proper use of food have a major effect on the human body. Many raw materials and food products spoil easily, considerably shortening their shelf life. Extending the shelf-life of food is required to remove seasonal consumption, enlarge availability to the consumer and decrease losses in perishable food products, resorting to some procedures to preserve their properties. If natural antioxidants are used as alternatives for synthetic antioxidants, customer claims for food without damaging residual substances can be satisfied, and the poultry industry would benefit considerably. The principal objective of this review was to describe and under light the current situation regarding the role and importance of the use of antioxidant compounds obtained from natural sources in order to improve the nutritional qualities of eggs following their inclusion in the feed of laying hens. Data from bibliographic sources are analyzed and presented. The characteristics of a number of 9 plants with antioxidant potential are described with an emphasis on their characteristics, utilization, and possible effects on egg quality, performance, and health.
Production performance and blood parameters of laying hens fed diets with rosehip and flaxseed meal ingredients

Abstract This study was conducted to test the effect of two levels of rosehip meal used together with flaxseed meal on weekly production performances and blood parameters of laying hens. For that, we conducted a trial for 5 weeks on 120 laying hens (Tetra SL hybrid), aged 29 weeks. All three diets had 17.50% crude protein and 2780Kcal/kg metabolizable energy, but compared to the control diet (Con) the experimental diets contained 1.5% rosehip meal and 7% flaxseed meal (Diet1) or, 3% rosehip meal and 7% flaxseed meal (Diet2). The results revealed that only feed intake was significantly (P=0.0001) affected, being lower in experimental groups. Among the haematological parameters, the heterophile were significantly higher (P=0.0377) in both Diet1 and Diet2 groups versus the Con group. From the biochemical parameters analysed, in the lipid profile, glucose and triglycerides were significantly lower in the Diet2 group compared with both the Diet1 and Con groups. Total bilirubin was significantly higher (P=0.0020) in the Diet1 group compared to the Con and Diet2 groups, while the lactate dehydrogenase was lower (P=0.0414) in the samples of the Diet2 group. Overall, the studied ingredients had a slight influence on production performances and improved some blood parameters, especially the lipid profile of the Diet2 group.
Concomitant effects of orchiectomy and intermittent hypoxia on hepatic oxidative stress, expression of flavin-containing monooxygenases and transcriptomic profile in mice

Intermittent hypoxia induces oxidative stress and alters hepatic metabolism, likely underlying the association of sleep apnea with non-alcoholic fatty liver disease. In male patients with sleep apnea, metabolic or liver diseases, the levels of testosterone are reduced, and in patients with metabolic diseases, low levels of testosterone are associated with oxidative stress. To assess potential interactions between testosterone and IH on hepatic oxidative stress we used sham-operated or orchiectomized (ORX) mice exposed to normoxia (Nx) or IH (6% O2, 12 cycles/h, 12h/day) for 2 weeks. The activity of prooxidant (NADPH oxidase – NOX), antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase – SOD, Cat, GPx), lipid peroxidation (MDA concentration) and the total concentration of glutathione (GSH) were measured in liver. IH induced a prooxidant profile of enzyme activity (lower SOD activity and higher NOX/SOD and NOX/Cat activity ratio) without altering hepatic MDA and GSH content. Using RNA sequencing followed by a pathway enrichment analysis we identified putative hepatic genes underlying the interactions between IH and testosterone. ORX and IH altered the expression of genes involved in oxidoreductase activities, cytochromes dependent pathways, and glutathione metabolism. Among the genes upregulated in ORX-IH mice, the flavin-containing monooxygenases (FMO) are particularly relevant since these are potent hepatic antioxidant that could help prevent overt oxidative stress in ORX-IH mice. Graphical Abstract
Ambulante Gesundheitszentren: ein Vergleich Deutschland — Schweiz

Ambulante Gesundheitszentren nehmen einen zunehmend höheren Stellenwert in der ambulanten Patientenversorgung ein, wenngleich über das Pro und Contra vielfach diskutiert wird. Das Ziel der vorliegenden Studie ist es, einen Einblick in die Anzahl und Struktur ambulanter Versorgungszentren am Beispiel der Schweiz zu geben. Anhand eigener Daten soll zudem ein selektiver Vergleich mit bestehenden Einrichtungen in Deutschland vorgenommen werden. Internetrecherche (Oktober – Dezember 2015) unter Nutzung der Suchmaschinen Google Scholar, Yahoo, PubMed Health, Trip Database und Cochrane Library mit den Suchwörtern „Gesundheitszentrum/-en“, „Partnerpraxen“, „Ärztehaus“ und „ambulante Behandlungszentren“. Erfasst wurden die gefunden Gesundheitszentren der Schweiz mit haus- und/oder spezialistischer Versorgung sowie deren Charakteristika (z.B. Investorengruppe, Stadt – Land). Die Ergebnisse wurden mit eigenen, bereits publizierten Zahlen verglichen. Die Suche ergab 59 vorhandene ambulante Gesundheitszentren in der Schweiz (49 städtische, 10 ländliche Versorgungsgebiete). Der überwiegende Teil der Gesundheitszentren wurde durch Investorenketten eingerichtet. Private Investoren bilden die Ausnahme und beschränken sich eher auf kleinere Einrichtungen in ländlichen Versorgungsregionen. Die durchschnittliche Anzahl der in den jeweiligen Gesundheitszentren tätigen Hausärzte betrug 3,22, die der rein fachärztlich tätigen Spezialisten 3,86). Es überwogen Internisten, gefolgt von Psychiatern, Orthopäden/Rheumatologen sowie Gynäkologen und Dermatologen (n = 44; 17; 15; 10). Die Anzahl der Hausärzte war im Vergleich zu den deutschen Einrichtungen etwas höher, die der Spezialisten etwas niedriger. Patienten, Leistungserbringer, Versicherer und politische Entscheidungsträger benötigen mehr und bessere Daten über ambulante Gesundheitszentren, um entsprechende Initiativen zu unterstützen.
Random Dynamical Systems with Jumps and with a Function Type Intensity

Abstract In paper [4] there are considered random dynamical systems with randomly chosen jumps acting on Polish spaces. The intensity of this process is a constant λ. In this paper we formulate criteria for the existence of an invariant measure and asymptotic stability for these systems in the case when λ is not constant but a Lipschitz function.
Viewpoints and Experiences of Family Physicians with Regard to the German Innovation Fund

Hintergrund Zur Verbesserung der medizinischen Versorgung innerhalb der gesetzlichen Krankenversicherung wurde 2015 der Innovationsfonds eingerichtet. Damit Interventionen und neue Versorgungsformen im Hinblick auf ihre Wirksamkeit erprobt und in die Regelversorgung übernommen werden können, bedarf es der Einbeziehung der allgemeinmedizinischen Breitenversorgung. Methoden Zwischen Oktober 2021 und Januar 2022 wurden 16 halbstandardisierte Einzelinterviews mit Hausärzt*innen geführt, die bereits an Innovationsfonds-Projekten teilgenommen haben (Förderlinie ‚Neue Versorgungsformen‘). Inhalte des Leitfadens waren: Einstellungen in Bezug auf Innovationsfonds-Projekte und deren Nutzen; Teilnahmebereitschaft und deren Voraussetzungen; Erfahrungen bei Teilnahme an spezifischen Projekten; wahrgenommene Optimierungspotenziale. Ergebnisse Die Interviewten verbinden den Innovationsfonds mehrheitlich mit Chancen und Potenzialen (u.a. Intensivierung anwendungsnaher Versorgungsforschung, unabhängige Finanzierung, Einbeziehung der Breitenversorgung). Dennoch sind sich viele Hausärzt*innen unsicher, inwiefern speziell die Primärversorgung längerfristig vom Innovationsfonds wird profitieren können. Die Bilanz, die in Folge der Teilnahme an den Versorgungsmodellen gezogen wird, ist mehrheitlich positiv (Nutzen der Intervention, Aufwand-Nutzen-Verhältnis). Dennoch werden auch Hürden und Belastungsfaktoren berichtet, etwa Dokumentationspflichten und Eingriffe in Praxisabläufe. Schlussfolgerungen Um die Teilnahmebereitschaft von Hausärzt*innen an Innovationsfonds-Projekten zu erhöhen, gilt es, die Hausarztkonformität von Projekten sicherzustellen, v.a. mit Blick auf ärztliche Entscheidungsspielräume, die Limitierung von Dokumentationspflichten, die Gewährleistung von Praxisroutinen, eine stärkere Involvierung in die Forschungsplanung sowie eine Aufwertung des hausärztlichen Settings. Schlüsselwörter Innovationsfonds; Versorgungsforschung; Hausarzt; Primärversorgung; Rekrutierung
Characterization of a repurposed antiviral for HSV-1 in Neural Progenitor Cells and Organoid models by

Herpes Simplex virus 1 (HSV-1) infection is a debilitating illness and can lead to severe complications such as encephalitis and keratitis if untreated. The global seropositivity rate of this virus in the population (ages 0 to 49) is 66.6%, making it a public health concern worldwide. The high prevalence of this disease makes the discovery of new treatments for HSV-1 essential. The “gold standard” antiviral, acyclovir, is a highly efficacious treatment for HSV-1 infection, however, prolonged use may lead to the development of strains of acyclovir-resistant HSV-1. These acyclovir-resistant strains have a greater potential to lead to complications especially in immunocompromised individuals, thus making the discovery of new antivirals paramount. There is an urgent need to identify novel anti-herpetic drugs that can inhibit acyclovir-resistant HSV-1 strains. Herein, we describe a computational method of drug repurposing to elicit potential new antivirals for HSV-1. This method was executed by utilizing transcriptomic data from neurons as input data to a database known as BaseSpace Correlation Engine (Illumina®) to generate a list of correlated pharmacological compounds. This list was then manually filtered to select one compound that was positively correlated (Retinoic acid) and supported the host immune system. Retinoic acid was tested in both 2D (Neural Progenitor cells) and 3D (organoid models), and resulted in no change in the presence of HSV-1 with an MOI of 0.1 in 02SF 2D cultures post-
Secure and Integrated Rank Ordered Multiple Keywords Search on Encrypted Data over Cloud

— As cloud computing is having increasing popularity, data owners have been motivated to outsource their sensitive data from local sites to the public cloud for great ease access and reduced cost. To preserve privacy of data, sensitive document has to be encrypted before outsourcing data to cloud. Thus, providing a search facility in an encrypted cloud data is of paramount importance. Since cloud consists of the huge number of data users and documents, it is required to search multiple keywords in the search request and to return documents based on the order of their relevance to these query keywords. The existing paper, for multiple keyword semantics provides a privacy requirement such as “coordinate matching” and “inner product similarity” for securing cloud data. In this paper, we propose integrity checking of rank order for multiple keywords search in an encrypted cloud data. To achieve integrity of rank we use a hash mapping technique so that the order of the rank will be preserved. We establish the strict privacy for securing cloud data utilization system by checking the integrity of rank order.
MATHEMATICAL ANALYSIS OF AN IMPROVED HEPATITIS B VIRUS MODEL

A hepatitis B virus (HBV) model with standard incidence and the uninfected cells growing logistically is investigated. By analyzing the corresponding characteristic equations, the local stability of the infection-free and infection equilibria is discussed, respectively. Further, the existence of an orbitally asymptotically stable periodic orbit is also studied. By means of the theory of competitive systems and compound matrices, sufficient conditions are derived for the global stability of the infection-free and infection equilibria, respectively. At last, numerical simulations are carried out to illustrate the main results.
Gepubliceerde zelfmoorden en verhoging van sterfte door zelfmoord en ongelukken in Nederland 1972-1980

To investigate the relationship between newspaper suicide coverage & mortality due to suicide & accidents, a partial replication is presented of the analyses by D. P. Phillips (see SA 23:1/75H1402 & SA 27:5/79K2733), using Dutch data from 1972 to 1980. Expected mortality rates were constructed by using Phillips's method & by regressing mortality rates on time & dummy variables for monthly effects. The results were inconclusive. For suicide & traffic accidents, mortality rates tend to rise 3%-8% on a monthly basis (about the same amount as Phillips showed), but this is not significant at conventional levels. No rise of mortality was revealed in the "other accidents" category. 2 Tables, 2 Graphs, 1 Appendix, 5 References. Modified HA.
Schoolse en niet-schoolse participatie bij jongeren: het profiel en de houdingen van participerende jongeren

Raising democratic and civic attitudes with youngsters receives more and more attention. Evidence for a strong connection between participation and democratic attitudes has been found in earlier research. In this study, concerning the in and out of school participation of adolescents, we find a similar positive relation. Nevertheless, we see that young people do not participate equally in associations outside school. But when they do get the opportunity to participate in school, the barriers to participation are smaller, Furthermore we can conclude that participation has a cumulative character: students who join activities at school, are more often involved in associational life. Participation at school, although it should be open to all students, reaches only a specific number and kind of pupils. Students in technical and vocational schools have less opportunities to participate. Therefore, we have to question somewhat the assumptions about the democratic strength of schools.
Attempts to limit sporulation in the probiotic strain Bacillus subtilis BG01-4TM through mutation accumulation and selection

The use of bacterial spores in probiotics over viable loads of bacteria has many advantages, including the durability of spores, which allows spore-based probiotics to effectively traverse the various biochemical barriers present in the gastrointestinal tract. However, the majority of spore-based probiotics developed currently aim to treat adults, and there is a litany of differences between the adult and infant intestinal systems, including the immaturity and low microbial species diversity observed within the intestines of infants. These differences are only further exacerbated in premature infants with necrotizing enterocolitis (NEC) and indicates that what may be appropriate for an adult or even a healthy full-term infant may not be suited for an unhealthy premature infant. Complications from using spore-based probiotics for premature infants with NEC may involve the spores remaining dormant and adhering to the intestinal epithelia, the out-competing of commensal bacteria by spores, and most importantly the innate antibiotic resistance of spores. Also, the ability of Bacillus subtilis to produce spores under duress may result in less B. subtilis perishing within the intestines and releasing membrane branched-chain fatty acids. The isolate B. subtilis BG01-4TM is a proprietary strain developed by Vernx Biotechnology through accumulating mutations within the BG01-4TM genome in a serial batch culture. Strain BG01-4TM was provided as a non-spore-forming B. subtilis , but a positive sporulation status for BG01-4TM was confirmed through in vitro testing and suggested that selection for the sporulation defective genes could occur within an environment that would select against sporulation. The durability of key sporulation genes was ratified in this study, as the ability of BG01-4TM to produce spores was not eliminated by the attempts to select against sporulation genes in BG01-4TM by the epigenetic factors of high glucose and low pH. However, a variation in the genes in isolate BG01-4-8 involved in the regulation of sporulation is believed to have occurred during the mutation selection from the parent strain BG01-4TM. An alteration in selected sporulation regulation genes is expected to have occurred from BG01-4TM to BG01-4-8, with BG01-4-8 producing spores within 24 h, ~48 h quicker than BG01-4TM.
Research Progress in Improving Photosynthetic Efficiency

Photosynthesis is the largest mass- and energy-conversion process on Earth, and it is the material basis for almost all biological activities. The efficiency of converting absorbed light energy into energy substances during photosynthesis is very low compared to theoretical values. Based on the importance of photosynthesis, this article summarizes the latest progress in improving photosynthesis efficiency from various perspectives. The main way to improve photosynthetic efficiency is to optimize the light reactions, including increasing light absorption and conversion, accelerating the recovery of non-photochemical quenching, modifying enzymes in the Calvin cycle, introducing carbon concentration mechanisms into C3 plants, rebuilding the photorespiration pathway, de novo synthesis, and changing stomatal conductance. These developments indicate that there is significant room for improvement in photosynthesis, providing support for improving crop yields and mitigating changes in climate conditions.