Miracle-dz/UniFAR-FTUs huggingface dataset:
CRISPR/Cas9 genome editing for neurodegenerative diseases

Gene therapy has emerged as a promising therapeutic strategy for various conditions, including blood disorders, ocular disease, cancer, and nervous system disorders. The advent of gene editing techniques has facilitated the ability of researchers to specifically target and modify the eukaryotic cell genome, making it a valuable tool for gene therapy. This can be performed through either in vivo or ex vivo approaches. Gene editing tools, such as zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR-Cas-associated nucleases, can be employed for gene therapy purposes. Among these tools, CRISPR-Cas-based gene editing stands out because of its ability to introduce heritable genome changes by designing short guide RNAs. This review aims to provide an overview of CRISPR-Cas technology and summarizes the latest research on the application of CRISPR/Cas9 genome editing technology for the treatment of the most prevalent neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic lateral sclerosis, and Spinocerebellar ataxia.
MAGNETIC FIELD DESIGN TO REDUCE SYSTEMATIC EFFECTS IN NEUTRON ELECTRIC DIPOLE MOMENT MEASUREMENTS

OF DISSERTATION MAGNETIC FIELD DESIGN TO REDUCE SYSTEMATIC EFFECTS IN NEUTRON ELECTRIC DIPOLE MOMENT MEASUREMENTS Charge-Conjugation (C) and Charge-Conjugation-Parity (CP) Violation is one of the three Sakharov conditions to explain via baryogenesis the observed baryon asymmetry of the universe (BAU). The Standard Model of particle physics (SM) contains sources of CP violation, but cannot explain the BAU. This motivates searches for new physics beyond the standard model (BSM) which address the Sakharov criteria, including high-precision searches for new sources of CPV in systems for which the SM contribution is small, but larger effects may be present in BSM theories. A promising example is the search for the electric dipole moment of the neutron (nEDM), which is a novel system to observe CPV due to the initial and final state being identical. A non-zero measurement necessarily requires violation of P and T discrete symmetries; invoking CPT invariance requires that CP is violated. There are BSM theories which predict a magnitude for the nEDM larger than SM predictions, so that such studies are beneficial at setting constraints on new physics. The current experimental limit of dn < 3.0 × 10−26 e cm at 90% CL as set by the Institut Laue-Langevin (ILL) [1] was largely limited by systematic effects related to the magnetic field. The research presented here supported technical progress toward a new measurement of the nEDM, with the goal of improving the result by an order of magnitude. A novel approach to the problem of limiting systematics is proposed, studied in Monte Carlo simulations, and an optimized prototype was constructed for use in a magnetic resonance experiment.
KELP GAMETOPHYTES IN CHANGING OCEANS

Kelp forests are ecologically diverse habitats that provide vast ecosystem goods and services, but are threatened by climate and anthropogenic stressors. Laminarian kelps have an alternating biphasic life cycle, and while there is a growing understanding of climate impacts on the macroscopic diploid sporophyte, impacts on the microscopic haploid gametophyte stage are just emerging. There exists a strong history of gametophyte literature on single species and environmental factors, but only recently studies have increasingly examined multiple climate stressors, species and populations. We synthesize studies on kelp gametophytes, building upon our understanding of their responses to environmental conditions and subsequent vulnerability in changing oceans. Kelp gametophytes have a broad tolerance to environmental conditions predicted to change with climate change, including temperature and irradiance, but large variation exists among species and populations. Key processes such as gametogenesis and early sporophyte development consistently appear more sensitive to environmental conditions than vegetative growth and may present bottlenecks to ongoing kelp persistence. Indirect effects of climate change negatively affect kelp gametophytes through competition, grazing and sedimentation. Modern genomic techniques are paving the way to transition research into field settings that include both sporophyte and gametophyte stages. Unravelling the response of gametophytes to changing environmental conditions is beginning to provide an understanding of overall kelp forest persistence and enables proactive conservation and management initiatives in changing oceans.
Outcomes of Primary Percutaneous Coronary Intervention in Post COVID-19 Patients presenting with ST Segment Elevation Myocardial Infarction

Background: COVID-19 can cause a wide range of thrombotic diseases, including acute coronary syndromes (ACS). While these thrombotic diseases occur during acute infection, evidence on the long-term thrombotic consequences of COVID-19 remain unknown. Objective: The aim of the current study was to establish the particular coronary angiographic findings, as well as the procedural and clinical effectiveness of revascularization in post COVID-19 patients presenting with STEMI. Patients and methods: A total 100 patients presented to Ain Shams University Hospitals with ST Segment Elevation Myocardial Infarction (STEMI) managed by primary percutaneous coronary intervention (PCI). Participants were divided into two groups: Group (A) included 50 patients who developed COVID-19 infection in the previous 6 months, and Group (B) included 50 patients who deny COVID-19 infection in the previous 6 months. Group (A) was divided into two subgroups: the Early Post-COVID subgroup, which included 16 patients who developed STEMI within 8 weeks of infection, and the Late Post-COVID subgroup, which included 34 patients who developed STEMI >8-24 weeks after infection. Results: The Early Post-COVID subgroup had a statistically significant high thrombus load on angiography, with 81.3% versus 48% in the control group. This resulted in a statistically significant increase in the utilization of pre-dilatation (56.2% versus 24%) and thrombus aspiration (43.8% versus 4%) in the Early Post-COVID grouping (P-values 0.015 and 0.001, respectively). Coronary no-reflow was a substantially more common in the Early post-COVID subgroup (62.5%) than in the control group (22%). This translated into a higher Major Adverse Cardiovascular Events (MACE) among Early Post-COVID patients, at 31.3% versus 6% in the control group. Conclusion: The thrombogenic impact of COVID-19 on STEMI outcomes continues even after infection clearance being greatest during the first 8 weeks following infection and thereafter diminishes. It has an impact on the angiographic, procedural, and overall clinical success of in-hospital revascularization.
A study of X-ray narrow emission lines from ionised gas in Active Galactic Nuclei

This thesis presents a study of X-ray narrow emission lines from ionised gas within Active Galactic Nuclei (AGN) and is based on the analysis of high- resolution X-ray spectra from XMM-Newton’s Reflection Grating Spectrome- ter. I have investigated the location, ionisation level and chemical abundances of the gas producing X-ray narrow emission lines in NGC 5548 and NGC 1365. Using observations during an unusual time when the nuclear emission of NGC 5548 is obscured by cool absorbing material, I show that the emission lines are consistent with being absorbed by further ionised gas within the AGN system. This is supported by the location I derive for the emission line gas, within those of the known warm absorbers in this system. With NGC 1365 spectra I demonstrate that these emission lines can be used to derive metal abundances within AGN systems; this can be especially important in obscured objects where usual methods of deriving abundances from UV spectra are unavailable due to the large degree of absorption. Finally, leading on from the results on individual objects, I explore outflow-inflow velocities of emission lines in a large sample of AGN, testing whether these velocities are distributed differently for different categories of AGN. With current observations it is not possible to detect population velocity differences in the X-ray narrow emission lines of the magnitude previously seen in optical studies. Through this study, I significantly increase the number of O VII f line detections using a uniform method across a sample of AGN. These three studies show the variety of insights into AGN environments enabled by the study of X-ray narrow emission lines. The thesis closes with a forward look to what could be achieved in this area with the next generation of X-ray observatories.
in Children – Albanian Experience

: Background: Urolithiasis is already one of the oldest diseases even in pediatric patients. The prevalence of kidney stones is estimated to be 1:20.000 children/year. The medium age of onset is between 5-7 years. The aim of our study was to see the characteristic features, predisposing factors and therapeutic procedures for urolithiasis in children, as well as metabolic abnormalities especially hypercalciuria. Methods: In a retrospective study we involved 216 patients (46% of them with family history for kidney stones) in an 8 year period (study period from 2007 till 2015). All patients underwent abdominal ultrasonography and laboratory examination. Results: Male/female ratio was 2.17:1. Diagnostic medium age was 6.44 years. The most frequent symptoms were: back pain in 33.3%, abdominal pain in 19.4%, gross hematuria in 19.4%, microhematuria in 14%, urinary retention in 9.7%, dysuria in 8.3%, vomiting in 11.1%, growth retardation in 4.2%, hypertension in 1.4%, spontaneous passage of renal calculi in 5.5%. 4.2% of patients were asymptomatic. Infectious stone was found in 47.2% of cases. The chemical composition of the stone was studied only in 51% of cases from which 56% appeared to be calcium oxalate and phosphate stones. Metabolic abnormalities were found in 50% of patients. Conclusions: Urolithiasis is common disease among Albanian children. 50% of patients had metabolic disorders. Calcium-oxalate and calcium-phosphate represents the most frequent types of calculi. Hypercalciuria is the most important metabolic disorder. Hypocitraturia is the risk factor to calcium urolithiasis. We recommend that every child with stone must be estimated for a metabolic screening.
Planning of Arrayed Waveguide Grating (AWG) for 16x16 Channels Transmission System of Dense Wavelength Division Multiplexing (DWDM)

Arrayed Waveguide Grating (AWG) Technology, one technique of dividing the channel into smaller sub-channels by adjusting the fixed array length increment. AWG techniques can generate coherent transmissions, which are suitable for Wavelength Division Multiplexing (WDM), to be implemented as multiplexer, demultiplexer, filter, add-drop device, and more. This paper discusses the design planning of AWG parameters operating on C-Band channels (1530-1560 nm), to support the needs of WDM channels, especially Dense-WDM (DWDM). Planning is done using WDM-Phasar tool and through theoretical calculations with MatLab. Theoretical calculations aim to produce ideal design parameters, while through WDM-phasar by adding the device size limit, crosstalk and nonuniformity values, it is expected to obtain more realistic design parameters. The parameters observed include the magnitude of the diffraction order (m), the length of the free propagation range (FPR), the difference in array length (ΔL), the number of arrays (Narray), number of I/O (Nmax) and free spectral range (FSR) channels. By using 16 channels of 100 GHz (0.8 nm) in the C-band, the size of the device (15000x9000 μm2), crosstalk (-35 dB) and nonuniformity (0.5), through WDM-Phasar assistance the AWG parameter 1197.347 μm (FPR), 23.764 μm (ΔL), 41 (m), 56 (N array ), 16 (N max ) and 11.2 nm (FSR). While ignoring the device size, crosstalk and non-uniformity variables, theoretical parameters were generated at 1308.61 μm, 25.1698 μm, 43.7143, 108 pieces, 27 channels and 21.211 nm, respectively for FPR, ΔL, m , Narray, Nmax and FSR. or WDM system capacity (16x16).
Sparse reconstruction with multiple Walsh matrices

Abstract The problem of how to find a sparse representation of a signal is an important one in applied and computational harmonic analysis. It is closely related to the problem of how to reconstruct a sparse vector from its projection in a much lower-dimensional vector space. This is the setting of compressed sensing, where the projection is given by a matrix with many more columns than rows. We introduce a class of random matrices that can be used to reconstruct sparse vectors in this paradigm. These matrices satisfy the restricted isometry property with overwhelming probability. We also discuss an application in dimensionality reduction where we initially discovered this class of matrices.
Customer service culture at AEON supermarkets in Vietnam

With strong competition and globalization trends like today, customers are faced with countless products, brands, prices, suppliers, etc. Businesses that want to survive and develop need to. Customer-oriented and customer-centric, bringing the highest value to customers. When customers have more demands on satisfying their needs, businesses need to have a strategy to develop close relationships with customers, which is also a way to maintain existing customer loyalty and build relationships with potential customers. AEON Vietnam is a supermarket system from Japan. Through research, thoroughly understand the needs and habits of customers, approach and communicate with customers, manage customer information in the most systematic and effective way in order to offer the best products and services, the most suitable service that meets the needs and wants of the market.
Plant Growth-Promoting Rhizobacteria Improve Rice Response to Climate Change Conditions

Rice is one of the most important crops in the world and is considered a strategic crop for food security. Furthermore, the excessive use of chemical fertilizers to obtain high yields causes environmental problems. A sustainable alternative includes taking advantage of beneficial bacteria that promote plant growth. Here, we investigate the effect of five bacterial biofertilizers from halophytes on growth, and we investigate photosynthetic efficiency in rice plants grown under saline conditions (0 and 85 mmol L−1 NaCl) and future climate change scenarios, including increased CO2 concentrations and temperature (400/700 ppm and 25/+4 °C, respectively). Biofertilizers 1–4 increased growth by 9–64% in plants grown with and without salt in both CO2- temperature combinations, although there was no significant positive effect on the net photosynthetic rate of rice plants. In general, biofertilizer 1 was the most effective at 400 ppm CO2 and at 700 ppm CO2 +4 °C in the absence of salt. Inocula 1–5 also stimulated plant length at high CO2 levels without salt. Finally, the positive effect of biofertilization was attenuated in the plants grown under the interaction between salt and high CO2. This highlights the significance of studying biofertilization under stress interaction to establish the real potential of biofertilizers in the context of climate change conditions.
An efficient global optimization scheme for building energy simulation based on linear radial basis function.

Multi-objective optimization is considerably increasing its importance in building design since the design goals are moving from the solely energy saving target to the whole building performance, comprehensive of energy, cost and comfort targets. Optimization algorithms coupled with building simulation codes are frequently used in academic researches. However, they are limitedly adopted in real building design due to the high number of expensive simulation runs required by optimization algorithms such as direct search methods, evolutionary algorithm, particle swarm optimization and hybrid algorithms. For this reason, an efficient optimization scheme is essential for the diffusion of the optimization tools in building performance design outside the academic world. The research focuses on the development of an Efficient Global Optimization (EGO) scheme based on a radial basis function network (RBFN) model to emulate the expensive evaluations of the building performance simulation (BPS). The test bed of the method is the optimal building refurbishment of three simplified module representative of existing buildings, for which the optimal solutions have been also calculated by using the brute force approach, i.e. evaluating the performance of all the possible combinations of the retrofit measures. Finally, the EGO performances were also compared with those offered by the popular Non Sorting Genetic Algorithm (NSGA-II). The results show the extent to which the EGO algorithm is able to find optimal solutions with a reduced number of expensive simulation runs. This capability makes the EGO algorithm suitable for the optimization of expensive simulation codes such as lighting models, CFD codes or dynamic simulation of building and HVAC systems.
Towards an optical clock for space: Compact, high-performance optical lattice clock based on bosonic atoms

Optical clocks operated on satellites are expected to open up new opportunities in time transfer, geodesy, fundamental physics, and satellite navigation. Here we demonstrate an important first step towards this goal: a modular, compact, optical lattice clock (OLC) system that achieves $2.0\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}17}$ fractional uncertainty. The clock is operated with bosonic strontium and improves the performance of bosonic OLCs by a factor of 30. This has important implications for future use of bosonic OLCs in fundamental physics and metrology. We make use of the clock's metrological performance to measure, with independent clocks, the isotope shift of the $^{1}S_{0}\ensuremath{\rightarrow}^{3}P_{0}$ transitions of $^{88}\mathrm{Sr}$ and $^{87}\mathrm{Sr}$, with an uncertainty of 12 mHz. The ratio of the transition frequencies is thus determined with $3\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}17}$ fractional uncertainty.
Adaptive trajectory tracking control for VTOL‐UAVs with unknown inertia, gyro‐bias, and actuator LOE

This paper proposes an adaptive controller for the trajectory tracking problem of vertical take‐off and landing unmanned aerial vehicles with unknown inertia and gyro‐bias, in the presence of external disturbances and actuator loss of effectiveness. To achieve our control objective, first, an a priori bounded virtual control law, providing an a priori bounded thrust and desired attitude, is designed for the translational dynamics. Thereafter, the torque input is designed for the rotational dynamics to track the desired orientation derived in the first stage. The stability of the overall hierarchical closed‐loop system and the global convergence of the tracking errors to zero are rigorously established. Finally, numerical simulations are performed to validate the effectiveness of the proposed control scheme.
Prevalence of smart phone addiction and its impact on sleep quality and selected psychological problems among nursing students of selected nursing colleges, Rohtas, Bihar

Introduction: Smartphones have become an integral part of youth. But at the same time addiction towards its use have become a serious concern among them. Objective: The main objective of the study is to determine the prevalence of smartphone addiction among the nursing students. Further the study was done to find out the association between the smartphone addiction with sleep quality and some selected psychological problems, along with its association with selected demographic variables. Research Approach: A quantitative research approach was adopted in which cross sectional research design was used by the researcher and the samples were chosen by convenient sampling technique. The nursing underwent various scales to assess the relationship between smartphone addiction, sleep quality and psychological problems. Result: The researcher reported that almost 77 students (36 male and 41 female) found to be addicted with smartphone usage. Further on, a fair positive significant association (0.226) was obtained between smartphone addiction and sleep quality. The researcher identified and mention that fair positive relationship found between smartphone addiction and psychological problems such as depression, anxiety and stress at the p level <0.001. Conclusion: Thus, the analysis suggested that there is a need to develop counselling programs where experts can raise the awareness of university students, to understand the Pons and cons of smartphone usage.
Effects of reverse geocoding on OpenStreetMap tag quality assessment

OpenStreetMap (OSM), a widely‐used open‐source geographic information system platform, provides a vast geographic dataset in which users contribute both geometric information (nodes, ways, and relations) and semantic information (tags). This method of voluntary contributions is governed by the collective effort of the users. It is widely acknowledged that the quantity of tag information is substantial, but its quality is often poor. Researchers are therefore trying to assess the quality of the tags and enhance the data through various integration experiments. This article investigates the validity of the tags for geographical objects in metropolitan areas using municipal data and a reverse geocoding technique. The proposed method evaluates the data quality and the matching process carried out by reverse geocoding, using municipal points of interest as a reference. The accuracy of the tag and address information and road network centrality metrics were assessed for the OSM objects that were matched to the locations of interest. The tags were found to match the points of interest with an accuracy of 88%. Furthermore, the tag values were categorized and analyzed based on their similarity. It is concluded that in metropolitan settings where centers of interest are closely located, the accuracy of tags and addresses tends to decrease.
An approach to determination of the criteria of harmony of biological objects

The paper aims to study the criteria of harmony by example of protective coloration for antelopes and dynamics of the parameters for human cardiovascular system at different stages of obesity. The criteria of harmony reflecting the adaptive mechanisms of the biological objects under consideration are obtained. In the case of antelopes (Taurotragus oryx), criteria of harmony chosen were colorimetric parameters of protective coloration, subjected to change due to the domestication of these animals. In the case of the human cardiovascular system, the parameters of heart rate, systolic blood pressure and diastolic blood pressure were selected. The results of the simulation show that the increase in body weight, due to hypodynamia in women, leads to a deterioration of the harmony of the dynamics of the cardiovascular system. As a criterion of harmony, the variety of combinations of values observed in the cycle of changes of these parameters built in the result of modeling is used. A similar dependence takes place in the example of protective coloration of antelopes. As a criterion of harmony, in this case, a variety of combinations of colorimetric parameters of the protective color of antelopes is used. The results of mathematical modeling obtained in the work allow us to propose a new approach to finding a universal criterion of harmony of living systems related to biodiversity and adaptation to living conditions.