Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell-based treatment of cerebral palsy: still a long way ahead.

BACKGROUND Cerebral palsy (CP) is a permanent neurodevelopmental disorder with considerable global disability. Various rehabilitation strategies are currently available. However, none represents a convincing curative result. Cellular therapy recently holds much promise as an alternative strategy to repair neurologic defects. METHOD In this narrative review, a comprehensive search of the MEDLINE and ClinicalTrials.gov was made, using the terms: "cell therapy" and "cerebral palsy", including published and registered clinical studies, respectively. RESULTS The early effects of these studies demonstrated that using cell therapy in CP patients is safe and improves the deficits for a variable duration. Despite such hopeful early bird results, the long-term outcomes are not conclusive. CONCLUSIONS Due to the heterogeneous nature of CP, personal factors seem essential to consider. Cell dosage, routes of administration, and repeated dosing are pivotal to establish optimal personalized treatments. Future clinical trials should consider employing other cell types, specific cell modifications before administration, and cell-free platforms.
Post-translational Modifications: The Signals at the Intersection of Exercise, Glucose Uptake, and Insulin Sensitivity

Abstract Diabetes is a global epidemic, of which type 2 diabetes makes up the majority of cases. Nonetheless, for some individuals, type 2 diabetes is eminently preventable and treatable via lifestyle interventions. Glucose uptake into skeletal muscle increases during and in recovery from exercise, with exercise effective at controlling glucose homeostasis in individuals with type 2 diabetes. Furthermore, acute and chronic exercise sensitizes skeletal muscle to insulin. A complex network of signals converge and interact to regulate glucose metabolism and insulin sensitivity in response to exercise. Numerous forms of post-translational modifications (eg, phosphorylation, ubiquitination, acetylation, ribosylation, and more) are regulated by exercise. Here we review the current state of the art of the role of post-translational modifications in transducing exercise-induced signals to modulate glucose uptake and insulin sensitivity within skeletal muscle. Furthermore, we consider emerging evidence for noncanonical signaling in the control of glucose homeostasis and the potential for regulation by exercise. While exercise is clearly an effective intervention to reduce glycemia and improve insulin sensitivity, the insulin- and exercise-sensitive signaling networks orchestrating this biology are not fully clarified. Elucidation of the complex proteome-wide interactions between post-translational modifications and the associated functional implications will identify mechanisms by which exercise regulates glucose homeostasis and insulin sensitivity. In doing so, this knowledge should illuminate novel therapeutic targets to enhance insulin sensitivity for the clinical management of type 2 diabetes.
Uphill sprinting load– and force–velocity profiling: Assessment and potential applications

ABSTRACT This study aimed to quantify the validity and reliability of load–velocity (LV) relationship of hill sprinting using a range of different hill gradients and to describe the effect of hill gradient on sprint performance. Twenty-four collegiate-level athletes performed a series of maximal sprints on either flat terrain or hills of gradients 5.2, 8.8 and 17.6%. Velocity–time curves were recorded using a radar device. LV relationships were established using the maximal velocity achieved in each sprinting condition, whilst force–velocity–power (FVP) profiles were established using only the flat terrain sprint. LV profiles were shown to be valid (R2 = 0.99) and reliable (TE < 4.4%). For every 1-degree increase in slope, subjects’ velocity decreased by 1.7 ± 0.1% on average. All the slopes used represented low resistance relative to the entire LV spectrum (<25% velocity loss). Subjects who exhibited greater horizontal force output at higher velocities on flat terrain were most affected by the gradient of the hill. Hills of gradients up to 17.6% do not provide sufficient resistance to optimize power development. However, such hills could be used to develop late-stage technical ability, due to the prolonged horizontally oriented body position that occurs as subjects attempt to overcome the acceleration due to gravity.
Human Physiological Signals Extraction and Estimation Using a Car Seat Built-in Piezo-film Sensors

The aim of the development is monitoring physiological parameters of car occupants (driver and passengers) without any constraint to detect their drowsiness or any unusual physiological states. The authors have developed a sensing system that allows any car occupants to feel free to move and behave. Piezoelectric-film sensors are embedded in the seat without changing the seating comfort impression of occupants. Since it is well known that piezoelectric sensors are sensitive to vibration, extracting physiological components from the sensor output is a challenge. To solve this issue, the authors combined a noise suppression step with transfer function estimation and physiological parameter estimation using an Extended Kalman Filter. The pulsation and breathing estimation and tracking are possible with very good performances.
High chromosomal mobility of rDNA clusters in holocentric chromosomes of Triatominae, vectors of Chagas disease (Hemiptera‐Reduviidae)

The subfamily Triatominae (Hemiptera‐Reduviidae) includes more than 150 blood‐sucking species, potential vectors of the protozoan Trypanosoma cruzi, causative agent of Chagas disease. A distinctive cytogenetic characteristic of this group is the presence of extremely stable chromosome numbers. Unexpectedly, the analyses of the chromosomal location of ribosomal gene clusters and other repetitive sequences place Triatominae as a significantly diverse hemipteran subfamily. Here, we advance the understanding of Triatominae chromosomal evolution through the analysis of the 45S rDNA cluster chromosomal location in 92 Triatominae species. We found the 45S rDNA clusters in one to four loci per haploid genome with different chromosomal patterns: On one or two autosomes, on one, two or three sex chromosomes, on the X chromosome plus one to three autosomes. The movement of 45S rDNA clusters is discussed in an evolutionary context. Our results illustrate that rDNA mobility has been relatively common in the past and in recent evolutionary history of the group. The high frequency of rDNA patterns involving autosomes and sex chromosomes among closely related species could affect genetic recombination and the viability of hybrid populations, which suggests that the mobility of rDNA clusters could be a driver of species diversification.
Nasopharyngeal Expression of Angiotensin-Converting Enzyme 2 and Transmembrane Serine Protease 2 in Children within SARS-CoV-2-Infected Family Clusters

ACE2 and TMPRSS2 are well established in the literature as SARS-CoV-2 entry factors. Recent data suggest that lower levels of nasal ACE2 in children may be associated with their lower incidence of coronavirus disease 2019 (COVID-19). ABSTRACT Lower levels of angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) in the nasal epithelium of children may be related to a lower incidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, compared to adults. However, no direct evidence is available to support this hypothesis. In this study, we compared the transcript levels of ACE2 and TMPRSS2 in nasopharyngeal swab samples (n = 234) from children and adult family members within SARS-CoV-2-exposed families and assessed the association with SARS-CoV-2 infection status. Transcript levels for ACE2, but not TMPRSS2, were higher in adults than in children (n = 129 adults and 105 children; P < 0.05). The expression of the two genes was not significantly different between SARS-CoV-2 positive and SARS-CoV-2 negative patients within the same age groups. However, in families with one or more SARS-CoV-2 positive adult family members, expression of both genes was significantly higher in SARS-CoV-2 positive children than in SARS-CoV-2 negative children (P < 0.05). By multivariate analysis, ACE2 expression adjusted for age and sex was significantly associated with SARS-CoV-2 infection in the overall population (odds ratio [OR], 1.112 [95% confidence interval [CI], 1.012 to 1.229]; P < 0.05). The degree of this association was higher (OR, 1.172 [95% CI, 1.034 to 1.347]; P < 0.05) in the subgroup of families with only SARS-CoV-2 positive adult family members. Our results suggest that children with lower levels of nasal ACE2 and TMPRSS2 are more likely to remain SARS-CoV-2 negative despite being exposed to a SARS-CoV-2 positive adult family member. IMPORTANCE ACE2 and TMPRSS2 are well established in the literature as SARS-CoV-2 entry factors. Recent data suggest that lower levels of nasal ACE2 in children may be associated with their lower incidence of coronavirus disease 2019 (COVID-19). In this study, using data from nasopharyngeal swab specimens from adult and pediatric members of families in which one or more members of the family had laboratory-confirmed SARS-CoV-2 infection, we show that children with lower levels of ACE2 and TMPRSS2 are more likely to remain SARS-CoV-2 negative despite being exposed to a SARS-CoV-2 positive adult family member. These results provide new insights into the roles of nasopharyngeal ACE2 and TMPRSS2 in acquiring SARS-CoV-2 infection, and they show that the differential expression of these genes in adults versus children may contribute to differential rates of SARS-CoV-2 infection in these populations.
Modeling the population‐level impact of opioid agonist treatment on mortality among people accessing treatment between 2001 and 2020 in New South Wales, Australia

Abstract Background and Aims The individual‐level effectiveness of opioid agonist treatment (OAT) in reducing mortality is well established, but there is less evidence on population‐level benefits. We use modeling informed with linked data from the OAT program in New South Wales (NSW), Australia, to estimate the impact of OAT provision in the community and prisons on mortality and the impact of eliminating excess mortality during OAT initiation/discontinuation. Design Dynamic modeling. Setting and participants A cohort of 49 359 individuals who ever received OAT in NSW from 2001 to 2018. Measurements Receipt of OAT was represented through five stages: (i) first month on OAT, (ii) short (1–9 months) and (iii) longer (9+ months) duration on OAT, (iv) first month following OAT discontinuation and (v) rest of time following OAT discontinuation. Incarceration was represented as four strata: (i) never or not incarcerated in the past year, (ii) currently incarcerated, (iii) released from prison within the past month and (iv) released from prison 1–12 months ago. The model incorporated elevated mortality post‐release from prison and OAT impact on reducing mortality and incarceration. Findings Among the cohort, mortality was 0.9 per 100 person‐years, OAT coverage and retention remained high (> 50%, 1.74 years/episode). During 2001–20, we estimate that OAT provision reduced overdose and other cause mortality among the cohort by 52.8% [95% credible interval (CrI) = 49.4–56.9%] and 26.6% (95% CrI =22.1–30.5%), respectively. We estimate 1.2 deaths averted and 9.7 life‐years gained per 100 person‐years on OAT. Prison OAT with post‐release OAT‐linkage accounted for 12.4% (95% CrI = 11.5–13.5%) of all deaths averted by the OAT program, primarily through preventing deaths in the first month post‐release. Preventing elevated mortality during OAT initiation and discontinuation could have averted up to 1.4% (95% CrI = 0.8–2.0%) and 3.0% (95% CrI = 2.1–5.3%) of deaths, respectively. Conclusion The community and prison opioid agonist treatment program in New South Wales, Australia appears to have substantially reduced population‐level overdose and all‐cause mortality in the past 20 years, partially due to high retention.
SlJMJ7 orchestrates tomato fruit ripening via crosstalk between H3K4me3 and DML2-mediated DNA demethylation.

The ripening of fleshy fruits is a unique developmental process that Arabidopsis and rice lack. This process is driven by hormones and transcription factors. However, the critical and early regulators of fruit ripening are still poorly understood. Here, we revealed that SlJMJ7, an H3K4 demethylase, is a critical negative regulator of fruit ripening in tomato. Combined genome-wide transcription, binding sites, histone H3K4me3 and DNA methylation analyses demonstrated that SlJMJ7 regulates a key group of ripening-related genes, including ethylene biosynthesis (ACS2, ACS4 and ACO6), transcriptional regulation (RIN and NOR) and DNA demethylation (DML2) genes, by H3K4me3 demethylation. Moreover, loss of SlJMJ7 function leads to increased H3K4me3 levels, which directly activates ripening-related genes, and to global DML2-mediated DNA hypomethylation in fruit, which indirectly prompts expression of ripening-related genes. Together, these effects lead to accelerated fruit ripening in sljmj7 mutant. Our findings demonstrate that SlJMJ7 acts as a master negative regulator of fruit ripening through not only direct removal of H3K4me3 from multiple key ripening-related factors, but also crosstalk between histone and DNA demethylation. These findings reveal a novel cross-talk between histone methylation and DNA methylation to regulate gene expression in plant developmental processes.
Which Speech Sound Norms Are Used in U.S. Public Schools? A Retrospective Survey Analysis

Abstract Normative data concerning speech sound acquisition are widely and heavily used in the United States to determine eligibility for services. Normative studies differ in the age and geographic location of participants, which can limit applicability across the United States. In 2016, we queried school-based speech-language pathologists (SLPs) in the United States using a web-based survey to determine which speech sound norms are used. Nine regions from the U.S. census were represented. The Iowa–Nebraska norms were most commonly reported. However, many SLPs were unable to name the source of the data that they used. More than 60% of the sample indicated that they referred to one source of normative data. Results of a logistic regression indicated that region of the United States predicted if SLPs used one or multiple sources. Specifically, SLPs in the West North Central region of the United States (which includes Iowa and Nebraska) were more likely to use only one source of data, the Iowa–Nebraska norms. We recommend that speech sound norms are less heavily relied upon for eligibility decisions, particularly due to the substantial variations in culture and dialect throughout the various regions of the United States.
An exploration of stakeholder perceptions to inform the development of an evidence-based classification system in para dressage

ABSTRACT In dressage, horse-rider combinations must demonstrate harmony whilst performing a test of gaits and movements, scored by judge(s) using predetermined criteria. The para dressage governing body is working towards compliance with the International Paralympic Committee’s mandate for evidence-based classification, which requires a comprehensive understanding of key performance determinants. This study aimed to explore stakeholder perceptions surrounding the key determinants of, and impact of impairment on, para dressage performance. Semi-structured interviews with 30 para dressage stakeholders (athletes, classifiers, judges, coach) were analysed using the Framework method. Themes relating to the equine and human athlete were associated with overall dressage performance and discussed within the context of impairment and horse-rider partnership. Key performance determinants were summarised as the athlete’s ability to maintain dynamic postural control for absorbing the horse’s movement and coordinating leg, hand, and seat aids, which directly influence the horse’s quality and accuracy of movements during dressage. Thus, muscular coordination, joint mobility that influences rider posture, and personality traits that influence the horse-rider partnership were considered performance determinants. These themes will inform the development of an evidence-based classification system, through the establishment of standardised, sport-specific performance measures for assessing the relationship between impairment and activity limitation in para dressage.
Detection of Early Gastric Cancer after Helicobacter pylori Eradication

Background: Based on evidence that Helicobacter pylori eradication reduces the development of gastric cancer and other diseases such as peptic ulcer, eradication therapy has prevailed. However, gastric cancer can develop even after successful eradication. Summary: In this review article, we searched for studies that identified the characteristics of primary and metachronous gastric cancers after H. pylori eradication, the risk factors for the development of these cancers after successful H. pylori eradication, and whether image-enhanced endoscopy is useful for diagnosing gastric cancer after eradication. A gastritis-like appearance is seen as a characteristic endoscopic finding, which corresponds to an epithelium with low-grade atypia – also known as nonneoplastic epithelium – covering the surface of the cancerous glands. This finding may make endoscopic detection of early gastric cancer difficult after H. pylori eradication. Similar risk factors, such as the male sex, endoscopic atrophy, histologic intestinal metaplasia, and late eradication, have been reported as predictors for the development of both primary and metachronous gastric cancers. Image-enhanced endoscopy, such as linked color imaging, may be useful for the detection and risk stratification of gastric cancer after eradication. Key Messages: Based on these findings, we believe that effective surveillance of high-risk patients leads to early detection of gastric cancer in the era of H. pylori eradication.
Improved BEC SMOS Arctic Sea Surface Salinity product v3.1

Abstract. Measuring salinity from space is challenging since the sensitivity of the brightness temperature (TB) to sea surface salinity (SSS) is low (about 0.5 K / psu), while the SSS range in the open ocean is narrow (about 5 psu, if river discharge areas are not considered). This translates into a high accuracy requirement of the radiometer (about 2–3 K). Moreover, the sensitivity of the TB to SSS at cold waters is even lower (0.3 K / psu), making the retrieval of the SSS in the cold waters even more challenging. Due to this limitation, ESA launched a speciﬁc initiative in 2019, the Arctic+Salinity project (AO/1-9158/18/I-BG), to produce an enhanced Arctic SSS product with better quality and resolution than the available products. This paper presents the methodologies used to produce the new enhanced Arctic SMOS SSS product (Martínez et al., 2020) . The product consists of 9-day averaged maps in an EASE 2.0 grid of 25 km. The product is freely distributed from the Barcelona Expert Center (BEC, http://bec.icm.csic.es/) with the DOI number: 10.20350/digitalCSIC/12620. The major change in this new product is its improvement of the effective spatial resolution that permits better monitoring of the mesoscale structures (larger than 50 Km), which beneﬁts the river discharges monitoring.
Prediction of Pancreatic Cancer in Diabetes Patients with Worsening Glycemic Control

Background: Worsening glycemic control indicates elevated risk of pancreatic ductal adenocarcinoma (PDAC). We developed prediction models for PDAC among those with worsening glycemic control after diabetes diagnosis. Methods: In 2000–2016 records within the Veterans Affairs Health System (VA), we identified three cohorts with progression of diabetes: (i) insulin initiation (n = 449,685), (ii) initiation of combination oral hypoglycemic medication (n = 414,460), and (iii) hemoglobin A1c (HbA1c) ≥8% with ≥Δ1% within 15 months (n = 593,401). We computed 12-, 36-, and 60-month incidence of PDAC and developed prediction models separately for males and females, with consideration of >30 demographic, behavioral, clinical, and laboratory variables. Models were selected to optimize Akaike's Information Criterion, and performance for predicting 12-, 36-, and 60-month incident PDAC was evaluated by bootstrap. Results: Incidence of PDAC was highest for insulin initiators and greater in males than in females. Optimism-corrected c-indices of the models for predicting 36-month incidence of PDAC in the male population were: (i) 0.72, (ii) 0.70, and (iii) 0.71, respectively. Models performed better for predicting 12-month incident PDAC [c-index (i) 0.78, (ii) 0.73, (iii) 0.76 for males], and worse for predicting 60-month incident PDAC [c-index (i) 0.69, (ii) 0.67, (iii) 0.68 for males]. Model performance was lower among females. For subjects whose model-predicted 36-month PDAC risks were ≥1%, the observed incidences were (i) 1.9%, (ii) 2.2%, and (iii) 1.8%. Conclusions: Sex-specific models for PDAC can estimate risk of PDAC at the time of progression of diabetes. Impact: Our models can identify diabetes patients who would benefit from PDAC screening.
Towards a roadmap for development of intelligent data analysis based cyber attack detection systems

Cyber-attacks against companies and governments are seeing an increase in complexity and persistence. These more complex attacks are aimed at penetrating corporate and government networks to obtain classified information. Common intrusion detection methods lack in their ability to detect such complex attacks. A framework is proposed to relate attack characteristics to analysis methods and business criteria. Traditional signature based detection algorithms still remain useful but the advanced approach of the attackers requires the use of intelligent data analysis. The framework is used as a roadmap to design a system capable of detection advanced persistent threats.
The role of natural antioxidants in the prevention of periodontal diseases

Background: Oxidative stress is a key causative factor in chronic inflammation and plays a key role in the pathogenesis of many chronic inflammatory diseases, including periodontitis. A properly balanced diet abundant in vegetables and fruits can be a rich source of biologically active substances that inhibit reactions with oxygen and ozone. The aim of the study is to assess the influence of natural antioxidants contained in vegetables and fruits on the occurrence and progression of periodontal diseases. Methods: The study covered a group of 368 students of the third grade (at the age of 18) of general secondary schools from the urban environment. The surveyed youth completed a survey regarding their dietary and hygienic habits. Subsequently, they participated in an oral cavity study in which the values of the plaque PL.I and gum GI index were assessed. Results: The beneficial effect on periodontal tissues resulted from eating of fruits and vegetables and frequent consumption of apples and carrots (median GI = 0). Eating of potato chips and crisps and the use of quick-service bars intensified gingivitis (median GI = 0.1). Conclusions: A diet intensifying oxidative stress influenced the deterioration the clinical status during the developmental age.