Miracle-dz/UniFAR-FTUs huggingface dataset:
The introduction and implementation of open dialogue in a day center in Athens, Greece: experiences and reflections of mental health professionals

Introduction The present study is part of a large-scale original action-research project aiming to assess the introduction and implementation of the Open Dialogue approach within the clinical practice of an established multidisciplinary team in a Day Centre in Athens, Greece. More specifically, it aimed to explore the experiences of professionals within the process of implementation both in relation to their clinical practice and their professional identity. Methods Data collection employed a focus group, which was set up to explore professional reflections of the implementation and research processes since the introduction of the model. Thematic Analysis of transcripts revealed two main themes that correspond to the impact of Open Dialogue on professionals’ clinical practice and on team dynamics, respectively. Results Professionals identify several challenges in implementing OD, such as difficulties in linking theory to practice, containing uncertainty, and addressing cultural barriers to dialogical ways of working. Professionals further reflect on their own internal journey stemming from the implementation of Open Dialogue that has led them to greater openness and growth, personally and as a team. Discussion The role of mental health professionals is being acknowledged as being at the frontline of any meaningful psychiatric reform through the assimilation and promotion of humanistic paradigms aiming towards a change of culture in psychiatric care across different contexts. Despite variations in implementation across different contexts, the importance of consolidating and embracing Open Dialogue as a philosophical framework underpinning mental health care is being discussed.
Editorial: Regulation of plant organelle biogenesis and trafficking

COPYRIGHT © 2023 Neuhaus, Pimpl, Zhao and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. TYPE Editorial PUBLISHED 26 May 2023 DOI 10.3389/fpls.2023. 1211807
Case report: A combination of mirror therapy and magnetic stimulation to the sacral plexus relieved phantom limb pain in a patient

Phantom limb pain (PLP) is a common sequela of amputation, experienced by 50-80% of amputees. Oral analgesics as the first-line therapy have limited effects. Since PLP usually affects activities of daily living and the psychological conditions of patients, effective treatments are imperatively needed. In this case study, a 49-year-old man was admitted to our hospital because of uncontrollable paroxysmal pain in his missing and residual leg. Due to severe injuries in a truck accident, the right lower limb of the patient was surgically amputated ~5 years ago. Around 1 month after amputation, he felt pain in his lost leg and PLP was diagnosed. Then, he started taking oral analgesics, but the pain still occurred. After admission on July 9, 2022, the patient received treatments of mirror therapy and magnetic stimulation to the sacral plexus. 1-month treatments reduced the frequency and intensity of pain in the phantom limb and the stump, without any adverse events. Analysis of high-resolution three-dimensional T1-weighted brain volume images at the end of 2-month treatments showed alterations in the thickness of cortex regions related to pain processing, compared to that before treatment. This case study gives us hints that one or both interventions of mirror therapy and sacral plexus magnetic stimulation effectively relieved PLP and stump limb pain. These non-invasive, low-cost and easily conducted treatments could be good options for PLP. But randomized controlled trials with a large number of cases are required to confirm their efficacy and safety.
Modelling spatiotemporal dynamics of cerebral blood flow using multiple-timepoint arterial spin labelling MRI

Introduction: Cerebral blood flow (CBF) is an important physiological parameter that can be quantified non-invasively using arterial spin labelling (ASL) imaging. Although most ASL studies are based on single-timepoint strategies, multi-timepoint approaches (multiple-PLD) in combination with appropriate model fitting strategies may be beneficial not only to improve CBF quantification but also to retrieve other physiological information of interest. Methods: In this work, we tested several kinetic models for the fitting of multiple-PLD pCASL data in a group of 10 healthy subjects. In particular, we extended the standard kinetic model by incorporating dispersion effects and the macrovascular contribution and assessed their individual and combined effect on CBF quantification. These assessments were performed using two pseudo-continuous ASL (pCASL) datasets acquired in the same subjects but during two conditions mimicking different CBF dynamics: normocapnia and hypercapnia (achieved through a CO2 stimulus). Results: All kinetic models quantified and highlighted the different CBF spatiotemporal dynamics between the two conditions. Hypercapnia led to an increase in CBF whilst decreasing arterial transit time (ATT) and arterial blood volume (aBV). When comparing the different kinetic models, the incorporation of dispersion effects yielded a significant decrease in CBF (∼10–22%) and ATT (∼17–26%), whilst aBV (∼44–74%) increased, and this was observed in both conditions. The extended model that includes dispersion effects and the macrovascular component has been shown to provide the best fit to both datasets. Conclusion: Our results support the use of extended models that include the macrovascular component and dispersion effects when modelling multiple-PLD pCASL data.
No evidence for tactile entrainment of attention

Temporal patterns in our environment provide a rich source of information, to which endogenous neural processes linked to perception and attention can synchronize. This phenomenon, known as entrainment, has so far been studied predominately in the visual and auditory domains. It is currently unknown whether sensory phase-entrainment generalizes to the tactile modality, e.g., for the perception of surface patterns or when reading braille. Here, we address this open question via a behavioral experiment with preregistered experimental and analysis protocols. Twenty healthy participants were presented, on each trial, with 2 s of either rhythmic or arrhythmic 10 Hz tactile stimuli. Their task was to detect a subsequent tactile target either in-phase or out-of-phase with the rhythmic entrainment. Contrary to our hypothesis, we observed no evidence for sensory entrainment in response times, sensitivity or response bias. In line with several other recently reported null findings, our data suggest that behaviorally relevant sensory phase-entrainment might require very specific stimulus parameters, and may not generalize to the tactile domain.
An anesthesia protocol for robust and repeatable measurement of behavioral visceromotor responses to colorectal distension in mice

Introduction Visceral motor responses (VMR) to graded colorectal distension (CRD) have been extensively implemented to assess the level of visceral pain in awake rodents, which are inevitably confounded by movement artifacts and cannot be conveniently implemented to assess invasive neuromodulation protocols for treating visceral pain. In this report, we present an optimized protocol with prolonged urethane infusion that enables robust and repeatable recordings of VMR to CRD in mice under deep anesthesia, providing a two-hour window to objectively assess the efficacy of visceral pain management strategies. Methods During all surgical procedures, C57BL/6 mice of both sexes (8–12 weeks, 25–35 g) were anesthetized with 2% isoflurane inhalation. An abdominal incision was made to allow Teflon-coated stainless steel wire electrodes to be sutured to the oblique abdominal musculature. A thin polyethylene catheter (Φ 0.2 mm) was placed intraperitoneally and externalized from the abdominal incision for delivering the prolonged urethane infusion. A cylindric plastic-film balloon (Φ 8 mm x 15 mm when distended) was inserted intra-anally, and its depth into the colorectum was precisely controlled by measuring the distance between the end of the balloon and the anus. Subsequently, the mouse was switched from isoflurane anesthesia to the new urethane anesthesia protocol, which consisted of a bout of infusion (0.6 g urethane per kg weight, g/kg) administered intraperitoneally via the catheter and continuous low-dose infusion throughout the experiment at 0.15–0.23 g per kg weight per hour (g/kg/h). Results Using this new anesthesia protocol, we systematically investigated the significant impact of balloon depth into the colorectum on evoked VMR, which showed a progressive reduction with increased balloon insertion depth from the rectal region into the distal colonic region. Intracolonic TNBS treatment induced enhanced VMR to CRD of the colonic region (>10 mm from the anus) only in male mice, whereas colonic VMR was not significantly altered by TNBS in female mice. Discussion Conducting VMR to CRD in anesthetized mice using the current protocol will enable future objective assessments of various invasive neuromodulatory strategies for alleviating visceral pain.
Case reports: a variety of clinical presentations and long-term evolution of Bochdalek hernias

Bochdalek hernias are the most common congenital diaphragmatic hernias, followed by Morgagni hernias. The failure of closure of the pleuroperitoneal membrane results in a posterolateral foramen, which can remain silent until adulthood. They remain a rare pathology with nearly a hundred cases published. Its clinical presentation is variable, making its diagnosis challenging for clinicians. Additionally, its symptoms are not necessarily representative of the content of the hernia. Its management is balanced between the abdominal and the thoracic approaches. However, no guidelines or algorithms are available to help surgeons in the decision-making process. We report here four consecutive cases of symptomatic Bochdalek hernias. Each case has a singular presentation, and we share how they were approached at our institution. In particular, this series shows no reoccurrence in 10+ years of follow-up in two cases and 20+ in one case, underlying the importance of surgical management when Bochdalek hernias are symptomatic.
Trained innate immunity, epigenetics, and food allergy

In recent years the increased incidence of food allergy in Western culture has been associated with environmental factors and an inappropriate immune phenotype. While the adaptive immune changes in food allergy development and progression have been well-characterized, an increase in innate cell frequency and activation status has also recently received greater attention. Early in prenatal and neonatal development of human immunity there is a reliance on epigenetic and metabolic changes that stem from environmental factors, which are critical in training the immune outcomes. In the present review, we discuss how trained immunity is regulated by epigenetic, microbial and metabolic factors, and how these factors and their impact on innate immunity have been linked to the development of food allergy. We further summarize current efforts to use probiotics as a potential therapeutic approach to reverse the epigenetic and metabolic signatures and prevent the development of severe anaphylactic food allergy, as well as the potential use of trained immunity as a diagnostic and management strategy. Finally, trained immunity is presented as one of the mechanisms of action of allergen-specific immunotherapy to promote tolerogenic responses in allergic individuals.
Remote-Care Interventions for Type 2 Diabetes Patients during the COVID-19 Pandemic

The COVID-19 pandemic poses increased burden on patients managing Type 2 diabetes. The Birmingham Free Clinic introduced new interventions as a result of the COVID-19 pandemic in efforts to mitigate pandemic burden on patients and to maintain care and diabetic disease management through medication delivery and virtual visitation appointments. Previous research shows diabetic education and lifestyle interventions can help patients with glycemic control in efforts to manage their diabetes. Before the pandemic, the clinic introduced grant-funded interventions focusing on providing glucose and blood pressure monitors as well as a social determinants of health screening during clinic visits. Of the clinic 300 patient population with Type 2 diabetes, 62 patients were selected for the dataset that had a baseline measurement of HbA1c and controlled blood pressure at 3 months leading up to the pandemic lockdown conditions in March 2020 with follow-up data collected in the 3 months preceding March 2021. Of those 62, 40 patients had complete data to analyze if there was a significant difference in HbA1c in a retrospective analysis. We observed a reduction of 0.39% (SD = 2.53, p-value = 0.1602) in HbA1c, which was not clinically meaningful nor statistically significant in showing an association of HbA1c reduction to the pandemic interventions for diabetes, with negligible differences in blood pressure control. By providing resources such as health screenings, medication deliveries, and telehealth options during the hardships of the pandemic, the clinic strives to maintain quality of care in diabetes management
Basal UPR activity in Aspergillus fumigatus regulates adaptation to nutrient stress and is critical for the establishment of corneal infection

The Aspergillus fumigatus unfolded protein response (UPR) is a two-component relay consisting of the ER-bound IreA protein, which splices and activates the mRNA of the transcription factor HacA. Spliced hacA accumulates under conditions of acute ER stress in vitro, and UPR null mutants are hypovirulent in a murine model of invasive pulmonary infection. In this report, we demonstrate that a hacA deletion mutant is completely unable to establish infection in a model of fungal keratitis, a corneal infection and an important cause of ocular morbidity and unilateral blindness worldwide. Contrary to our initial prediction, however, we demonstrate that hacA splicing is not increased above baseline conditions in the cornea, nor is the expression of genes classically associated with UPR activation, such as protein chaperones. We employed transcriptomics on wild-type and ΔhacA strains in gelatin media, as a proxy for the corneal environment, and found that hacA supports the expression of numerous primary and secondary metabolic processes that likely promote adaptation to nutrient limitation. Taken together, our results support a model in which the cornea, similar to growth on protein in vitro, is a source of sub-acute ER stress for A. fumigatus, but one nevertheless that requires the UPR pathway for proper adaptation. The data also suggest that this pathway could be a target for novel antifungals that improve visual outcomes for fungal keratitis patients. AUTHOR SUMMARY Fungal keratitis has emerged as a leading cause of ocular morbidity and unilateral blindness worldwide. Relative to other infectious contexts, however, little is known about the fungal genes or pathways that regulate invasive growth and virulence in the corneal environment. In this report, we demonstrate that genetic disruption of the Aspergillus fumigatus unfolded protein response (UPR) abolishes the ability of the mold to establish infection in a mouse model of FK. Despite this critical role for virulence, however, we did not detect a concerted activation of the pathway beyond levels observed on standard medium, suggesting that the host environment is not an acute source of endoplasmic reticulum stress. Transcriptomic profiling of the wild-type and UPR-deficient strains under host-relevant nutrient conditions revealed a critical role for the pathway in regulating primary and secondary metabolism, cell wall biology, and mitochondrial function, all of which likely modulate fungal growth within and interactions with the host. These results expand our understanding of UPR regulation and function in this important mold pathogen and suggest the pathway could serve as a target for novel antifungals that improve visual outcomes in the setting of fungal keratitis.
MULTIRELATIONAL MODELS OF LAZY, MONODIC TREE, AND PROBABILISTIC KLEENE ALGEBRAS

This paper studies basic properties of multirelations, and then shows that classes of multirelations provides models of three weaker variants of Kleene algebras, namely, lazy, monodic tree, and probabilistic Kleene algebras. Also it is shown that these classes of up-closed multirelations need not be models of Kozen’s Kleene algebras unlike the case of ordinary binary relations.
Analyzing monotone space complexity via the switching network model

Space complexity is the study of how much space/memory it takes to solve problems. Unfortunately, proving general lower bounds on space complexity is notoriously hard. Thus, we instead consider the restricted case of monotone algorithms, which only make deductions based on what is in the input and not what is missing. In this thesis, we develop techniques for analyzing monotone space complexity via a model called the monotone switching network model. Using these techniques, we prove tight bounds on the minimal size of monotone switching networks solving the directed connectivity, generation, and k-clique problems. These results separate monotone analgoues of L and NL and provide an alternative proof of the separation of the monotone NC hierarchy first proved by Raz and McKenzie. We then further develop these techniques for the directed connectivity problem in order to analyze the monotone space complexity of solving directed connectivity on particular input graphs. Thesis Supervisor: Jonathan A. Kelner Title: Associate Professor
Herpes Zoster Vaccination: Live or Inactivated Vaccines? Or no Vaccination at All?

Frage Derzeit stehen zwei Impfstoffe gegen Herpes zoster zur Verfügung, Zostavax® als attenuierter Lebendimpfstoff und Shingrix® als adjuvantierter, rekombinanter Totimpfstoff. Soll überhaupt gegen Herpes zoster geimpft werden, und wenn ja, wer, ab welchem Alter und mit welchem Impfstoff? Antwort Beide Impfstoffe sind effektiv in der Prävention von Herpes zoster und postherpetischer Neuralgie (PHN), mindestens für drei bis vier Jahre. Für den Lebendimpfstoff zeigte sich aber in Langzeitstudien ein rascher Effektivitätsverlust. Langzeitdaten für den Totimpfstoff liegen noch nicht vor. In den ersten vier Jahren war die Impfeffektivität des Totimpfstoffs derjenigen des Lebendimpfstoffs überlegen und der Effektivitätsverlust fiel geringer aus. Dieser Vorteil wird aber durch eine höhere Rate an lokalen und systemischen Nebenwirkungen erkauft. In Anbetracht der eher schlechten Verträglichkeit der Impfung muss in einer partizipativen informierten Entscheidung mit den Patient*innen diskutiert werden, ob eine Impfung erfolgen soll. Die STIKO empfiehlt die Impfung mit dem Totimpfstoff ab dem 60. Lebensjahr. In Deutschland wird die mit 226,80 Euro nicht ganz billige Impfung durch die Sozialversicherung bezahlt, in Österreich nicht.
Diagnostic accuracy of circulating exosomal circRNAs in malignances: A meta-analysis and systematic review

Background: Pathogenesis of malignant tumors are often accompanied by aberrant expression of circular RNAs (circRNAs), indicating the potential diagnostic value of circRNAs in tumors. CircRNAs have been found to be enriched, stable and ubiquitous in serum and plasma exosomes. The study aims at evaluating the diagnostic performance of circulating (plasma and serum) exosomal circRNA in different types of cancer by synthesis of published data. Methods: A comprehensive literature search was conducted in PubMed, Embase, Medline and the Web of Science databases to identify potentially eligible studies published before April 2021. We conducted the meta-analysis following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations. Results: Eleven articles comprising 21 studies were included, and a total of 1609 cases and 1498 controls were evaluated. Six types of cancer were involved in these studies, including lung cancer, hepatocellular carcinoma, colorectal cancer, gastric cancer, multiple myeloma and osteosarcoma. The pooled sensitivity and specificity were 0.72 (95% confidence interval [CI], 0.62–0.81) and 0.83 (95% CI, 0.78–0.88), respectively. Summary receiver operating characteristic curve was constructed and the pooled value of area under curve was 0.86 (95% CI, 0.83–0.89), indicating a favorable diagnostic efficacy of circulating exosomal circRNAs in malignancies. Conclusions: In conclusion, our study evaluated the diagnostic power of circulating exosomal circRNAs in 6 types of cancer by synthesis of published data comprising 21 studies from eleven articles. The pooled analysis provided the evidence supporting circulating exosomal circRNAs as a promising noninvasive diagnostic biomarkers for malignancies.
Evaluation Strategies for Florida's Early Childhood Comprehensive Systems Impact Project

Early Childhood Comprehensive Systems (ECCS) grants help states and communities improve early childhood systems to support child development, equitable access to quality services and supports, and family well-being. Truly interdisciplinary, ECCS promotes cross-sector systems change initiatives that focus on integrating health care, mental health, social services, and family support broadly and within communities with concentrated risk factors that negatively impact child health and development. Florida was 1 of 12 states funded from 2016 to 2021 to use a Collaborative Improvement and Innovation Network (CoIIN) approach to do this work. Through community-driven initiatives that focus on policy, partnerships, and practices, Florida ECCS committed to ensuring equitable opportunities for families and young children who may face barriers due to residing in disenfranchised communities. This article describes the process of evaluating this multiyear community-led, place-based initiative that leveraged cross-sector state-level support and used a variety of methods to track evolving ECCS processes and outcomes.
Some Smooth Compactly Supported Tight Framelets

For any dilation matrix with integer entries, we construct a family of smooth compactly supported tight wavelet frames in $L^2(\mathbb{R}^d)$, $d\ge 1$. Estimates for the degrees of smoothness of these framelets are given. Our construction involves some compactly supported refinable functions, the Oblique Extension Principle and a slight generalization of a theorem of Lai and Stockler.