Miracle-dz/UniFAR-FTUs huggingface dataset:
Bacterioplankton seasonality in deep high-mountain lakes

Due to global warming, shorter ice cover duration might drastically affect the ecology of lakes currently undergoing seasonal surface freezing. High-mountain lakes show snow-rich ice covers that determine contrasting conditions between ice-off and ice-on periods. We characterized the bacterioplankton seasonality in a deep high-mountain lake ice-covered for half a year. The lake shows a rich core bacterioplankton community consisting of three components: (i) an assemblage stable throughout the year, dominated by Actinobacteria, resistant to all environmental conditions; (ii) an ice-on-resilient assemblage dominating during the ice-covered period, which is more diverse than the other components and includes a high abundance of Verrucomicrobia; the deep hypolimnion constitutes a refuge for many of the typical under-ice taxa, many of which recover quickly during autumn mixing; and (iii) an ice-off-resilient assemblage, which members peak in summer in epilimnetic waters when the rest decline, characterized by a dominance of Flavobacterium, and Limnohabitans. The rich core community and low random elements compared to other relatively small cold lakes can be attributed to its simple hydrological network in a poorly-vegetated catchment, the long water-residence time (ca. 4 years), and the long ice-cover duration; features common to many headwater deep high-mountain lakes.
Transcatheter tricuspid valve interventions: Current status and future perspectives

The development of transcatheter therapies to treat valvular heart diseases has changed the rules of the game, particularly in the case of aortic stenosis and mitral regurgitation. In the last years, the tricuspid valve has also been the focus of these percutaneous techniques for several reasons: (1) tricuspid regurgitation is a frequent disease associated with poor clinical outcomes in spite of medical treatment, leading to end-stage right ventricular heart failure, (2) surgical tricuspid valve repair or replacement has been the treatment of choice for patients with tricuspid valve disease, but high mortality rates for isolated surgical tricuspid valve intervention have been reported in the literature, and (3) most patients with tricuspid pathology are ultimately denied cardiac surgery because of their comorbidity burden. Thus, in this context the development of less invasive catheter-based therapies would be of high clinical relevance. The present review provides an overview regarding the framework of chronic tricuspid regurgitation transcatheter therapeutic options, summarizes the transcatheter systems under clinical use or clinical evaluation with their technical features, and describes the updated current evidence in this challenging and evolving field.
Coronary access following ACURATE neo implantation for transcatheter aortic valve-in-valve implantation: Ex vivo analysis in patient-specific anatomies

Background Coronary access after transcatheter aortic valve implantation (TAVI) with supra-annular self-expandable valves may be challenging or un-feasible. There is little data concerning coronary access following transcatheter aortic valve-in-valve implantation (ViV-TAVI) for degenerated surgical bioprosthesis. Aims To evaluate the feasibility and challenge of coronary access after ViV-TAVI with the supra-annular self-expandable ACURATE neo valve. Materials and methods Sixteen patients underwent ViV-TAVI with the ACURATE neo valve. Post-procedural computed tomography (CT) was used to create 3D-printed life-sized patient-specific models for bench-testing of coronary cannulation. Primary endpoint was feasibility of diagnostic angiography and PCI. Secondary endpoints included incidence of challenging cannulation for both diagnostic catheters (DC) and guiding catheters (GC). The association between challenging cannulations with aortic and transcatheter/surgical valve geometry was evaluated using pre and post-procedural CT scans. Results Diagnostic angiography and PCI were feasible for 97 and 95% of models respectively. All non-feasible procedures occurred in ostia that underwent prophylactic “chimney” stenting. DC cannulation was challenging in 17% of models and was associated with a narrower SoV width (30 vs. 35 mm, p < 0.01), STJ width (28 vs. 32 mm, p < 0.05) and shorter STJ height (15 vs. 17 mm, p < 0.05). GC cannulation was challenging in 23% of models and was associated with narrower STJ width (28 vs. 32 mm, p < 0.05), smaller transcatheter-to-coronary distance (5 vs. 9.2 mm, p < 0.05) and a worse coronary-commissural overlap angle (14.3° vs. 25.6o, p < 0.01). Advanced techniques to achieve GC cannulation were required in 22/64 (34%) of cases. Conclusion In this exploratory bench analysis, diagnostic angiography and PCI was feasible in almost all cases following ViV-TAVI with the ACURATE neo valve. Prophylactic coronary stenting, higher implantation, narrower aortic sinus dimensions and commissural misalignment were associated with an increased challenge of coronary cannulation. Graphical Abstract Central illustration: Evaluating feasibility and challenge of coronary cannulation after ViV-TAVI with ACURATE neo valve.
The glycolytic enzyme ALDOA and the exon junction complex protein RBM8A are regulators of ribosomal biogenesis

Cellular growth is a fundamental process of life and must be precisely controlled in multicellular organisms. Growth is crucially controlled by the number of functional ribosomes available in cells. The production of new ribosomes depends critically on the activity of RNA polymerase (RNAP) II in addition to the activity of RNAP I and III, which produce ribosomal RNAs. Indeed, the expression of both, ribosomal proteins and proteins required for ribosome assembly (ribosomal biogenesis factors), is considered rate-limiting for ribosome synthesis. Here, we used genetic screening to identify novel transcriptional regulators of cell growth genes by fusing promoters from a ribosomal protein gene (Rpl18) and from a ribosomal biogenesis factor (Fbl) with fluorescent protein genes (RFP, GFP) as reporters. Subsequently, both reporters were stably integrated into immortalized mouse fibroblasts, which were then transduced with a genome-wide sgRNA-CRISPR knockout library. Subsequently, cells with altered reporter activity were isolated by FACS and the causative sgRNAs were identified. Interestingly, we identified two novel regulators of growth genes. Firstly, the exon junction complex protein RBM8A controls transcript levels of the intronless reporters used here. By acute depletion of RBM8A protein using the auxin degron system combined with the genome-wide analysis of nascent transcription, we showed that RBM8A is an important global regulator of ribosomal protein transcripts. Secondly, we unexpectedly observed that the glycolytic enzyme aldolase A (ALDOA) regulates the expression of ribosomal biogenesis factors. Consistent with published observations that a fraction of this protein is located in the nucleus, this may be a mechanism linking transcription of growth genes to metabolic processes and possibly to metabolite availability.
Chromosome 1q21 gain is an adverse prognostic factor for newly diagnosed multiple myeloma patients treated with bortezomib-based regimens

Chromosome 1q21 aberration is one of the most common cytogenetic abnormalities in multiple myeloma, and is considered an important prognostic factor. The present study analyzed the clinical relevance and prognostic impact of 1q21 gain in 194 patients with newly diagnosed multiple myeloma treated with bortezomib-based regimens. 1q21 gain was detected in 45.9% (89/194) of patients, and those with 1q21 gain had a worse prognosis. Strikingly, our results showed that excluding the effects of other coinciding genetic anomalies, patients carrying at least four copies of 1q21 had worse survival outcome. Moreover, del(13q) strongly correlates with 1q21 gain, and the coexistence of del(13q) and 1q21 gain plays an important role in reducing PFS and OS times. Therefore, 1q21 gain should be considered a high-risk feature in multiple myeloma patients treated with a bortezomib-based regimen.
Does board diversity reduce the probability of financial distress? Evidence from Chinese firms

This paper empirically investigates the impact of cognitive board diversity in education, expertise, and tenure facets on financial distress likelihood in the emerging economy of China. This study examines how this relationship varies across State-Owned Enterprises (SOEs) and Non-State-Owned Enterprises (NSOEs). Paper argues that the Chinese stock market, as a typical emerging market, is an excellent laboratory for studying the impact of board diversity on the probability of financial distress. Its underdeveloped financial system and inadequate investor protection leave firms unprotected from financial hardship. A sample of 12,366 observations from 1,374 firms from 2010 to 2018 shows that cognitive diversity qualities are positively linked with Z-score, implying that directors with different educational backgrounds, financial skills, and tenures can assist in reducing the probability of financial distress. Cognitive board diversity reduces the likelihood of financial distress in SOEs and NSOEs. However, tenure diversity is insignificant in all cases. Furthermore, the robustness model “two-step system Generalized Methods of Moments (GMM)” demonstrated a positive association between educational diversity, financial expertise, and financial distress scores. The results have significant implications for researchers, managers, investors, regulators, and policymakers.
Initial sirolimus dosage recommendations for pediatric patients with PIK3CD mutation-related immunodeficiency disease

Sirolimus is used to treat pediatric patients with PIK3CD mutation-related immunodeficiency disease. However, the initial dosages of sirolimus remain undecided. The present study aims to explore initial dosages in pediatric patients with PIK3CD mutation-related immunodeficiency disease. Pediatric patients with this disease were analyzed using the population pharmacokinetic (PPK) model and the Monte Carlo simulation. Body weight and concomitant use of posaconazole were included in the final PPK model, where, under the same weight, clearances of sirolimus were 1 : 0.238 between children without and children with posaconazole. Without posaconazole, the initial dosages of sirolimus were 0.07, 0.06, 0.05, and 0.04 mg/kg/day for body weights of 10–14, 14–25, 25–50, and 50–60 kg, respectively. With posaconazole, the initial dosages of sirolimus were 0.02 mg/kg/day for body weights of 10–60 kg. This is the first attempt to build a sirolimus PPK model for recommending initial dosages in children with PIK3CD mutation-related immunodeficiency disease, thereby providing a reference for individualized clinical drug administration.
Microbial and metabolomic insights into the bovine lipometabolic responses of rumen and mammary gland to zymolytic small peptide supplementation

Small peptides provide the easily utilized nitrogen for rumen microbial and promote acetate generation for milk fat synthesis. However, the impacts of peptide supplements on lipometabolic processes were still unclear. Therefore, a total of 800 multiparous dairy herds (with an average live weight of 667.6 ± 39.4 kg, an average lactation of 89.3 ± 18.8 days, and an average calving parity of 2.76 ± 0.47) were randomly allocated to the control (CON) and the small peptide (SP) supplement (100 g/day for each cow) treatments, respectively. A 35-day-long feeding procedure that includes a 7-day-long pretreatment test and a 28-day-long treatment test was followed for all cows. Dry matter intake (DMI) was recorded every day and calculated by the deviation between the supply and residue, while the daily milk production was automatically recorded through the rotary milking facilities. Milk samples were collected from each replicate on the last day, followed by the milk quality and milk lipid composition measurement. Rumen fluid samples were collected on the last day through esophageal tubing 3 h after morning feeding for the determination of the underlying mechanism of the small peptide on lipid metabolism through the measurement of rumen lipometabolic-related metabolites and rumen bacterial communities. Results indicated that dry matter intake showed an increasing trend, while milk production and the milk fat content remarkably increased after SP supplement (P < 0.05). Further detailed detection showed the mainly increased milk composition focused on monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA). Acetate-producing microbes, such as Acetitomaculum, Bifidobacterium, Succiniclasticum, and Succinivibrio, and butyrate-producing microbes, such as Shuttleworthia and Saccharofermentans, significantly proliferated, which causatively brought the increased ruminal content of acetate, isobutyrate, and butyrate after SP supplement (P < 0.05) compared with CON. Lipometabolic metabolites such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), triacylglycerol (TG), and Acetyl-CoA also significantly increased after SP supplement. In summary, SP supplements help to increase milk fat content through the proliferation of rumen bacterial communities, which provided more acetate and butyrate for milk fat synthesis combined with the promotion of ruminal lipometabolism.
Editorial: Functional fitness/high intensity functional training for health and performance

Strength Training and Neuromuscular Performance Research Group (STreNgthP), Camilo José Cela University, Madrid, Spain, Federal University of Mato Grosso do Sul, Campo Grande, Brazil, Collegue of Healthcare Sciences, James Cook University, Townsville, QLD, Australia, Research and Development Department, iLOAD Solutions, Campo Grande, Brazil, EFFECTS Research Group, Department of Physiology, Faculty of Medicine, University of Granada, Granada, Spain, PROFITH “PROmoting FITness and Health Through Physical Activity” Research Group, Department of Physical Education and Sport, Faculty of Sport Sciences, University of Granada, Granada, Spain
Fusion de classifieurs en utilisant la théorie de l'évidence pour l'amélioration de la classification d'image

The problem addressed in this paper concerns the improvement of the image classification in conditions of insufficiency of deterministic and reliable prior information about the state and the nature of the image formation at the moment of acquisition. A fusion method of classifiers for a better classification of the observed scenes is proposed. The method is based on the theory of evidence. It is general and applicable to all types of classifiers. Using the rate of reliability of classification as criteria of evaluation, the obtained results, using the simulated and real remotely sensed data, show that the proposed method gives the best results compared with those of classifiers separately considered.
Design and Development of Electrogastrograph Wireless Electrode Module

Electrogastrography is a pain free diagnosing method for evaluating stomach myoelectric activity. People are drawn to EGG because it is painless diagnostic procedure. However, given to its non-invasive characteristics, there has been significant debate about the validity and applicability of EGG. The major goal of this project is to decouple the electrode-equipment probe connection. This enhances the patient’s mobility (infant, athlete, handicapped) while lowering probe-to-electrode misconnection. This portable electrode system allows physicians and nurses to examine data of a patient from a distance, decreasing the need for them to be physically present behind the subject for examination. The signal capture, data acquisition unit and communication interface with the Wireless Fidelity module are utilized to quantify stomach activity. Arduino is used to take an analogue signal and convert it to a digital value. The Arduino programming language is used to build the programmers, which are subsequently embedded in the board. As a consequence, the value retrieved from the Electrogastrograph module is transferred over Wireless Fidelity module to the thing speak online platform. Arduino accepts an analogue signal and transforms it to a digital value. The Arduino programming language is used to build the applications, which are subsequently incorporated into the board. As a result, the value retrieved from the EGG module is transferred through Wireless Fidelity module to thing speak online platform.
Adaptable medical device with 3D printing facilities

3D printing technology has breakthrough many long pending medical challenges. In this study the researchers are addressing epilepsy, a disability that limits mobility freedom, that can appear at any age but usually start in childhood or people over 60 years old. Diagnosing epilepsy quickly can be challenging due to the fact other conditions such as migraines, panic attacks and fainting possess similar symptoms. Regularly, it cannot be confirmed until seizure is detected. Electroencephalogram (EEG) is the most common test used to diagnose epilepsy. Epileptiform brain activity presence is used as a change seen on an EEG recording among epilepsy patients. The availability of EEG device for epilepsy diagnosis is currently limited to clinical settings which restricts the treatment process. The objective of this study is to offer an option for personalized home-based EEG device for epilepsy diagnosis and monitoring. A customized 3D printed EEG headset with 8 channel dry electrodes device is assembled and configured. The customization is managed by offering three different printable headset sizes with material selection options. The device is supported with an OpenBCI application connected through Bluetooth for recording and further processing options. The proposed device has potential to address number of limitations including the recent pandemic’s challenge where hospitalization option is restricted. The outcome of the research is expected to bring a new breakthrough in brain activity related research and clinical diagnosis in patient monitoring. The customization option of this device is also expected to offer a new trend in managing treatment compliance and adherence in clinical practice.
Subsatellite measurements of the lunar magnetic field

The Apollo 15 subsatellite magnetometer data have been used to map the lunar magnetic field over a narrow band of the lunar surface. Within this band the magnetic field is generally stronger and more variable over the farside highlands than the nearside maria. The correspondence between the strong variable lunar field regions and the source regions for limb compressions suggests that limb compressions arise as the result of the deflection of the solar wind just upstream of the terminator by the lunar magnetic field. Using this apparent relationship between field strength and limb compression source regions, it is deduced that the field strength in the northern farside highlands is not as strong as in the southern hemisphere at similar longitudes. Simultaneous measurements of the interplanetary magnetic field obtained by Explorer 35 and the Apollo 15 subsatellite above the dayside hemisphere are essentially identical. Thus, both instruments are measuring the undisturbed interplanetary field.
Orthogonality of linear (alinear) quasigroups and their parastrophes

Necessary and sufficient conditions of orthogonality of left(right) linear (alinear) quasigroups in various combinations are given. As corollary we obtain conditions of parastroph orthogonality of left (right) linear (alinear) quasigroups. Any linear (alinear) quasigroup over the group Sn (n 6 2;6) is not orthogonal to its (12)-parastrophe. 2000 Mathematics Subject Classification: 20N05
An integrated model of skill in solving elementary word problems cognition and instruction

Solving arithmetic word problems links the ability to execute arithmetic operations and the ability to apply these operations in real-word situations. An appropriate recent interest in word problems has produced much data on the relative difficulty of different kinds of word problems, and some data on the strategies children use to solve these problems together with the kinds of errors they make. We also have accounts of problem difficulty based on descriptions of the problem, that is, on their structural variables or on their semantic structure. We present here and account of problem difficulty based on a description of the psychological processes of the child. The purpose of this article is to outline a model of these processes, made explicit in the form of a computer-implemented model. The model solves many common word problems by acting them out with representations of physical counters. More difficult problems require augmenting this procedure first with knowledge that one object is a member of both .
A novel self-adaptive differential evolution algorithm for efficient design of multiplier-less low-pass FIR filter

Variety of real-world optimization problems can be successfully solved by employing a powerful technique, called Differential Evolution (DE) algorithm. The popularity of DE has grown tremendously since its inception as it includes a very few number of control parameters. However, the selection or tuning of these parameters plays a crucial role in determining the performance of the algorithm in terms of its convergence behaviour. In this paper, a novel Self-Adaptive DE (SADE) approach has been proposed for the de sign of a multiplier-less low-pass linear-phase FIR filter to improve the computational efficiency of the algorithm. For this purpose, the convergence behaviour of the SADE technique has been presented and it has been compared with that of traditional DE technique. Additionally, the performance of the SADE-optimized filter has been evaluated in terms of its magnitude response. The corresponding magnitude response for the DE-optimized filter has also been presented for comparison. It has been established that the proposed SADE algorithm outperforms the traditional DEfor this particular design problem.
Patients with Chronic Obstructive Pulmonary Disease and Evidence of Eosinophilic Inflammation Experience Exacerbations Despite Receiving Maximal Inhaled Maintenance Therapy

Background Some patients with chronic obstructive pulmonary disease (COPD) experience frequent exacerbations despite maximal inhaled therapy (“triple therapy”), possibly leading to high health care resource utilization (HCRU). Aim Describe characteristics, future HCRU, and mortality of patients with COPD who experience frequent exacerbations despite triple therapy; characterize individuals who may be candidates for biologic therapies. Methods This descriptive observational study used primary care data of patients aged ≥40 years in the United Kingdom receiving maintenance therapy for COPD who had ≥1 year of data prior to index date and ≥1 year of follow-up data. We described these patients’ clinical and demographic characteristics, including blood eosinophil counts (BEC), pattern of exacerbations, hospitalizations, and corticosteroid exposure, as well as future exacerbations, hospitalizations, and death. Results Of 43,753 patients with maintenance-treated COPD, 6480 experienced exacerbations despite ≥3 months of triple therapy. Of these, 5669 had available BEC: 1287 (22.7%) had BEC ≥250 cells/µL and ≥3 exacerbations in the year prior to the index date; 471 (36.6%) received ≥4 acute courses of oral corticosteroids. Patients with a pattern of high disease burden continued to have high disease burden: 51.1% experienced ≥3 exacerbations and 2.6% experienced ≥3 hospitalizations. Patients who experienced exacerbations despite triple therapy had a significantly higher risk of COPD-related death than other maintenance-treated patients (5.8% vs 2.1%). Conclusion Nearly one-quarter of patients receiving triple therapy for COPD who experienced frequent exacerbations had elevated BEC and ≥3 exacerbations, suggesting a potential mechanism of persistent eosinophilic inflammation that could be a target for eosinophil-depleting biologic therapy.