Miracle-dz/UniFAR-FTUs huggingface dataset:
Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean

Soybean (Glycine max) is an important crop in agricultural production where water shortage limits yields in soybean. Root system plays important roles in water-limited environments, but the underlying mechanisms are largely unknown. In our previous study, we produced a RNA-seq dataset generated from roots of soybean at three different growth stages (20-, 30-, and 44-day-old plants). In the present study, we performed a transcriptome analysis of the RNA-seq data to select candidate genes with probable association with root growth and development. Candidate genes were functionally examined in soybean by overexpression of individual genes using intact soybean composite plants with transgenic hairy roots. Root growth and biomass in the transgenic composite plants were significantly increased by overexpression of the GmNAC19 and GmGRAB1 transcriptional factors, showing up to 1.8-fold increase in root length and/or 1.7-fold increase in root fresh/dry weight. Furthermore, greenhouse-grown transgenic composite plants had significantly higher seed yield by about 2-fold than control plants. Expression profiling in different developmental stages and tissues showed that GmNAC19 and GmGRAB1 were most highly expressed in roots, displaying a distinct root-preferential expression. Moreover, we found that under water-deficit conditions, overexpression of GmNAC19 enhanced water stress tolerance in transgenic composite plants. Taken together, these results provide further insights into the agricultural potential of these genes for development of soybean cultivars with improved root growth and enhanced tolerance to water-deficit conditions.
Determination of optimal load in the Wingate Anaerobic Test is not depend on number of sprints included in mathematical models

Determining the optimal load (OPTLOAD) in measuring mechanical peak power output (PPO) is important in assessment of anaerobic fitness. The main goals of this study were: 1) to examine estimated optimal load and PPO based on a force-velocity test and 2) to compare the PPO from the previous method with the Wingate Anaerobic Test (WAnT). The study involved 15 academic male athletes, aged 22.4 ± 2.3 (years), height 178.9 ± 6.8 (cm), and body weight 77.9 ± 12.2 (kg). They performed the 30-s WAnT (7.5% of body weight) during the first visit to the laboratory. Second to fourth session included a force-velocity test (FVT) involving three, 10-s all-out sprints. A randomized load ranging from 3 to 11 kg was used in each session for FVT. The OPTLOAD and PPO were computed using quadratic relationships based on power-velocity (P-v) and power-percent of body weight (P-%BM) and including three, four, five and nine sprints from FVT. The results showed non-difference in OPTLOAD [13.8 ± 3.2 (%BM); 14.1 ± 3.5 (%BM); 13.5 ± 2.8 (%BM); 13.4 ± 2.6 (%BM)] executed at three, four, five, and nine sprints (F3,56 = 0.174, p = 0.91, η2 = 0.01). The two-way ANOVA revealed that PPO were similar between tested models (P-%BM vs. P-v) independently from the numbers of sprints (F3,112 = 0.08, p = 0.99, η2 = 0.000). Moreover, the PPO measured in the WAnT (870.6 ± 179.1 W) was significantly lower compared with in P-v model (1,102.9 ± 242.5–1,134.2 ± 285.4 W) (F4,70 = 3.044, p = 0.02, η2 = 0.148). In addition, the PPO derived from P-%BM model (1,105.2 ± 245.5–1,138.7 ± 285.3 W) was significantly higher compared with the WAnT (F4,70 = 2.976, p = 0.02, η2 = 0.145). The findings suggest the potential utility of FVT for assessment of anaerobic capacity.
Editorial: Rising stars in cancer metabolism 2022

COPYRIGHT © 2023 Izquierdo-Garcia and Scumaci. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. TYPE Editorial PUBLISHED 31 May 2023 DOI 10.3389/fonc.2023. 1223630
3D bioprinted hydrogel/polymer scaffold with factor delivery and mechanical support for growth plate injury repair

Introduction: Growth plate injury is a significant challenge in clinical practice, as it could severely affect the limb development of children, leading to limb deformity. Tissue engineering and 3D bioprinting technology have great potential in the repair and regeneration of injured growth plate, but there are still challenges associated with achieving successful repair outcomes. Methods: In this study, GelMA hydrogel containing PLGA microspheres loaded with chondrogenic factor PTH(1–34) was combined with BMSCs and Polycaprolactone (PCL) to develop the PTH(1–34)@PLGA/BMSCs/GelMA-PCL scaffold using bio-3D printing technology. Results: The scaffold exhibited a three-dimensional interconnected porous network structure, good mechanical properties, biocompatibility, and was suitable for cellchondrogenic differentiation. And a rabbit model of growth plate injury was appliedto validate the effect of scaffold on the repair of injured growth plate. The resultsshowed that the scaffold was more effective than injectable hydrogel in promotingcartilage regeneration and reducing bone bridge formation. Moreover, the addition ofPCL to the scaffold provided good mechanical support, significantly reducing limbdeformities after growth plate injury compared with directly injected hydrogel. Discussion: Accordingly, our study demonstrates the feasibility of using 3D printed scaffolds for treating growth plate injuries and could offer a new strategy for the development of growth plate tissue engineering therapy.
Editorial: Variation in plant strategies with levels of forest disturbance

COPYRIGHT © 2023 Chaturvedi, Bhadouria and Singh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Editorial: Variation in plant strategies with levels of forest disturbance
Wool cortisol as putative retrospective indicator of stress in ewes during the third trimester of pregnancy, and their newborns: effects of parity and litter size — an exploratory study

Stress is an important factor in animal welfare. Hair or wool cortisol concentrations are considered to be potential long-term indicators of stress experienced by an animal. Using Swifter sheep, we investigated whether ewe parity and litter size affect the wool cortisol concentrations in ewes and their offspring. We hypothesized that multiparous ewes and their offspring would have higher wool cortisol concentrations than primiparous ewes and their offspring, that ewes with larger litters and their offspring would have lower wool cortisol concentrations than ewes with smaller litters and their offspring, that male lambs would have higher wool cortisol concentrations than female lambs, and that the wool cortisol concentrations in the wool of ewes and their lambs would be correlated. Lamb wool grows in utero during the third trimester of pregnancy. In ewes, the shave–reshave method was used so that wool samples from ewes also covered approximately the last trimester of pregnancy. Our study confirmed that litter size affected ewe wool cortisol concentrations: ewes that gave birth to larger litters (i.e., 3 or 4 lambs) had higher wool cortisol concentrations than ewes that gave birth to smaller litters (i.e., 1 or 2 lambs). There was no evidence that the wool cortisol concentrations of the ewes and their lambs were correlated. Neither litter size nor parity of the ewe affected wool cortisol in the lambs. Our study confirms that wool cortisol can be reliably measured in ewes and their newborn lambs, and suggests that it may be useful as a retrospective indicator of stress during the last trimester of pregnancy.
Clinical profiling of specific diagnostic subgroups of women with chronic pelvic pain

Introduction Chronic pelvic pain (CPP) is a common condition affecting up to 26.6% of women, with many suffering for several years before diagnosis and/or treatment. Its clinical presentation is varied and there are frequently comorbid conditions both within and outside the pelvis. We aim to explore whether specific subgroups of women with CPP report different clinical symptoms and differing impact of pain on their quality of life (QoL). Methods The study is part of the Translational Research in Pelvic Pain (TRiPP) project which is a cross-sectional observational cohort study. The study includes 769 female participants of reproductive age who completed an extensive set of questions derived from standardised WERF EPHect questionnaires. Within this population we defined a control group (reporting no pelvic pain, no bladder pain syndrome, and no endometriosis diagnosis, N = 230) and four pain groups: endometriosis-associated pain (EAP, N = 237), interstitial cystitis/bladder pain syndrome (BPS, N = 72), comorbid endometriosis-associated pain and BPS (EABP, N = 120), and pelvic pain only (PP, N = 127). Results Clinical profiles of women with CPP (13–50 years old) show variability of clinical symptoms. The EAP and EABP groups scored higher than the PP group (p < 0.001) on the pain intensity scales for non-cyclical pelvic pain and higher than both the BPS and PP groups (p < 0.001) on the dysmenorrhoea scale. The EABP group also had significantly higher scores for dyspareunia (p < 0.001), even though more than 50% of sexually active participants in each pain group reported interrupting and/or avoiding sexual intercourse due to pain in the last 12 months. Scores for the QoL questionnaire (SF-36) reveal that CPP patients had significantly lower QoL across all SF-36 subscales (p < 0.001). Significant effects were also observed between the pain groups for pain interference with their work (p < 0.001) and daily lives (p < 0.001), with the EABP suffering more compared to the EAP and PP groups (p < 0.001). Discussion Our results demonstrate the negative impact that chronic pain has on CPP patients' QoL and reveal an increased negative impact of pain on the comorbid EABP group. Furthermore, it demonstrates the importance of dyspareunia in women with CPP. Overall, our results demonstrate the need for further exploration of interventions targeting QoL more broadly and suggest that novel approaches to classifying women with CPP are needed.
Minimally invasive surgery vs. open thoracotomy for non-small-cell lung cancer with N2 disease: a systematic review and meta-analysis

Background This meta-analysis aimed to investigate the effectiveness and safety of minimally invasive surgery [MIS, including robotic-assisted thoracoscopic surgery (RATS) and video-assisted thoracoscopic surgery (VATS)] and open thoracotomy (OT) for non-small cell lung cancer (NSCLC) patients with N2 disease. Methods We searched online databases and studies from the creation of the database to August 2022, comparing the MIS group to the OT group for NSCLC with N2 disease. Study endpoints included intraoperative outcomes [e.g., conversion, estimated blood loss (EBL), surgery time (ST), total lymph nodes (TLN), and R0 resection], postoperative outcomes [e.g., length of stay (LOS) and complication], and survival outcomes [e.g., 30-day mortality, overall survival (OS), and disease-free survival (DFS)]. We estimated outcomes using random effects meta-analysis to account for studies with high heterogeneity (I2 > 50 or p < 0.05). Otherwise, we used a fixed-effect model. We calculated odds ratios (ORs) for binary outcomes and standard mean differences (SMDs) for continuous outcomes. Treatment effects on OS and DFS were described by hazard ratio (HR). Results This systematic review and meta-analysis of 15 studies on MIS vs. OT for NSCLC with N2 disease included 8,374 patients. Compared to OT, patients that underwent MIS had less estimated blood loss (EBL) (SMD = – 64.82, p < 0.01), shorter length of stay (LOS) (SMD = −0.15, p < 0.01), higher R0 resection rate (OR = 1.22, p = 0.049), lower 30-day mortality (OR = 0.67, p = 0.03), and longer overall survival (OS) (HR = 0.61, P < 0.01). The results showed no statistically significant differences in surgical time (ST), total lymph nodes (TLN), complications, and disease-free survival (DFS) between the two groups. Conclusion Current data suggest that minimally invasive surgery may provide satisfying outcomes, a higher R0 resection rate, and better short-term and long-term survival than open thoracotomy. Systematic review registration https://www.crd. york.ac. uk/PROSPERO/, identifier: CRD42022355712.
Viewing river corridors through the lens of critical zone science

River corridors integrate the active channels, geomorphic floodplain and riparian areas, and hyporheic zone while receiving inputs from the uplands and groundwater and exchanging mass and energy with the atmosphere. Here, we trace the development of the contemporary understanding of river corridors from the perspectives of geomorphology, hydrology, ecology, and biogeochemistry. We then summarize contemporary models of the river corridor along multiple axes including dimensions of space and time, disturbance regimes, connectivity, hydrochemical exchange flows, and legacy effects of humans. We explore how river corridor science can be advanced with a critical zone framework by moving beyond a primary focus on discharge-based controls toward multi-factor models that identify dominant processes and thresholds that make predictions that serve society. We then identify opportunities to investigate relationships between large-scale spatial gradients and local-scale processes, embrace that riverine processes are temporally variable and interacting, acknowledge that river corridor processes and services do not respect disciplinary boundaries and increasingly need integrated multidisciplinary investigations, and explicitly integrate humans and their management actions as part of the river corridor. We intend our review to stimulate cross-disciplinary research while recognizing that river corridors occupy a unique position on the Earth's surface.
An updated catalog of CTCF variants associated with neurodevelopmental disorder phenotypes

Introduction CTCF-related disorder (CRD) is a neurodevelopmental disorder (NDD) caused by monoallelic pathogenic variants in CTCF. The first CTCF variants in CRD cases were documented in 2013. To date, 76 CTCF variants have been further described in the literature. In recent years, due to the increased application of next-generation sequencing (NGS), growing numbers of CTCF variants are being identified, and multiple genotype-phenotype databases cataloging such variants are emerging. Methods In this study, we aimed to expand the genotypic spectrum of CRD, by cataloging NDD phenotypes associated with reported CTCF variants. Here, we systematically reviewed all known CTCF variants reported in case studies and large-scale exome sequencing cohorts. We also conducted a meta-analysis using public variant data from genotype-phenotype databases to identify additional CTCF variants, which we then curated and annotated. Results From this combined approach, we report an additional 86 CTCF variants associated with NDD phenotypes that have not yet been described in the literature. Furthermore, we describe and explain inconsistencies in the quality of reported variants, which impairs the reuse of data for research of NDDs and other pathologies. Discussion From this integrated analysis, we provide a comprehensive and annotated catalog of all currently known CTCF mutations associated with NDD phenotypes, to aid diagnostic applications, as well as translational and basic research.
Place Cells: The Brain Cells That Help us Navigate the World

Navigation through the space around us is one of the most fundamental and crucial abilities that humans and other animals have. This ability is so natural that we usually do it easily, without even thinking about it. Though finding our way through our environments seems effortless, it actually requires a complex, fascinating mechanism—the navigation system in the brain. In this article, we will explore a major group of cells that are part of this navigation system, called place cells. As you read, you will discover how the trait of curiosity helps the brain’s navigation system, and you will learn some important lessons from taxi drivers in London!
Emotional arousal and valence in patients with fibromyalgia: a pilot study

The pathogenesis of pain in fibromyalgia is still not completely understood. A disrupted emotional modulation could affect the physiology of nociception and contributes to an altered perception of pain. The aim of this study was to test the role of emotional arousal and valence in pain susceptibility in fibromyalgia using the International Affective Picture System (IAPS) paradigm and the Fibromyalgia Severity Scale (FSS). The study focused on comparing emotional arousal and valence between patients with fibromyalgia and the control group. The secondary objective was to examine the correlation between emotional indices and scores on the FSS and the duration of the disease. The 20 patients with fibromyalgia enrolled showed a higher mean arousal score for all the stimuli, including a higher score for unpleasant and socially unpleasant stimuli. The valence scores for social-relevant stimuli were also higher. Increased arousal to unpleasant and socially unpleasant images and increased valence of them correlated with the duration of the disease and the severity of symptoms and could reflect impairment in social cognition and marked sensitivity to pain in interaction with central nociceptive dysregulation.
Sex differences in olfactory cortex neuronal loss in aging

Introduction Aging plays a major role in neurodegenerative disorders such as Alzheimer’s disease, and impacts neuronal loss. Olfactory dysfunction can be an early alteration heralding the presence of a neurodegenerative disorder in aging. Studying alterations in olfaction-related brain regions might help detection of neurodegenerative diseases at an earlier stage as well as protect individuals from any danger caused by loss of sense of smell. Objective To assess the effect of age and sex on olfactory cortex volume in cognitively healthy participants. Method Neurologically healthy participants were divided in three groups based on their age: young (20–35 years; n = 53), middle-aged (36–65 years; n = 66) and older (66–85 years; n = 95). T1-weighted MRI scans acquired at 1.5 T were processed using SPM12. Smoothed images were used to extract the volume of olfactory cortex regions. Results ANCOVA analyses showed significant differences in volume between age groups in the olfactory cortex (p ≤ 0.0001). In women, neuronal loss started earlier than in men (in the 4th decade of life), while in men more substantial neuronal loss in olfactory cortex regions was detected only later in life. Conclusion Data indicate that age-related reduction in the volume of the olfactory cortex starts earlier in women than in men. The findings suggest that volume changes in olfaction-related brain regions in the aging population deserve further attention as potential proxies of increased risk of neurodegenerative diseases.
Editorial: Advances in biofabrication for skin regeneration

Centre for Rapid and Sustainable Product Development, Instituto Politécnico de Leiria, Marinha Grande, Portugal, Department of Orthopaedic Surgery and Musculoskeletal Medicine Centre for Bioengineering and Nanomedicine, University of Otago Christchurch, Christchurch, New Zealand, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal
Metagenomic surveillance and comparative genomic analysis of Chlamydia psittaci in patients with pneumonia

Chlamydia psittaci, a strictly intracellular bacterium, is an underestimated etiologic agent leading to infections in a broad range of animals and mild illness or pneumonia in humans. In this study, the metagenomes of bronchoalveolar lavage fluids from the patients with pneumonia were sequenced and highly abundant C. psittaci was found. The target-enriched metagenomic reads were recruited to reconstruct draft genomes with more than 99% completeness. Two C. psittaci strains from novel sequence types were detected and these were closely related to the animal-borne isolates derived from the lineages of ST43 and ST28, indicating the zoonotic transmissions of C. psittaci would benefit its prevalence worldwide. Comparative genomic analysis combined with public isolate genomes revealed that the pan-genome of C. psittaci possessed a more stable gene repertoire than those of other extracellular bacteria, with ~90% of the genes per genome being conserved core genes. Furthermore, the evidence for significantly positive selection was identified in 20 virulence-associated gene products, particularly bacterial membrane-embedded proteins and type three secretion machines, which may play important roles in the pathogen-host interactions. This survey uncovered novel strains of C. psittaci causing pneumonia and the evolutionary analysis characterized prominent gene candidates involved in bacterial adaptation to immune pressures. The metagenomic approach is of significance to the surveillance of difficult-to-culture intracellular pathogens and the research into molecular epidemiology and evolutionary biology of C. psittaci.
The 2021 Madrilenian regional election: how can the incumbent improve its results in times of crisis?

Introduction Although there is a considerable amount of work on the effect of catastrophes on elections, we still do not have much work on the impact of the COVID-19 pandemic on them. This article focuses on the case of the Madrilenian regional election of 2021, looking for the causes of the improvement of the ruling party's results, the Popular Party (PP), which went from having 22.23% of the vote share in 2019 to 44.76% in 2021, and more concretely to the role that COVID-19 had on this. This election is especially interesting for this matter because the main issue was the question of how to manage the pandemic: The right-wing parties (and mainly the PP) criticized the restrictions imposed by the central government, led by the socialist Pedro Sánchez, while the left-wing parties defended them. Methods The article runs separate analyses at the aggregate and individual levels. At the aggregate level, it uses municipal and district-level data with electoral and socio-demographic variables; at the individual level, it uses a post-electoral survey. Results The main factors behind the PP's improvement were the absorption of its main competitor, Ciudadanos, the mobilization of previously abstentionist voters, and the impact of COVID-19 itself, with a higher propensity to increase their vote for the PP in the most affected areas. Discussion The article contributes to the understanding of the electoral consequences of the pandemic, providing some evidence that in this case, there was a rejection of the restrictions.