Miracle-dz/UniFAR-FTUs huggingface dataset:
A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein

Hemocytes are essential components of the immune system against invading pathogens in shrimp. Many uncharacterized transcripts exist in hemocytes but the knowledge of them is very limited. In the present study, we identified a novel small protein from the uncharacterized transcripts in hemocytes of Litopenaeus vannamei. This transcript was specifically expressed in hemocytes and encoded a novel secretory protein, which was designated as hemocyte-specific small protein (LvHSSP). The expression level of LvHSSP was significantly up-regulated in the hemocytes of shrimp infected with white spot syndrome virus (WSSV). After knockdown of LvHSSP by RNA interference, the WSSV copy number in shrimp decreased significantly. Conversely, WSSV copy number increased in shrimp when they were infected by WSSV after incubation with recombinant LvHSSP protein. These results suggested that LvHSSP might promote viral infection in shrimp. Immunocytochemical assay showed that the recombinant LvHSSP protein was located on the membrane of hemocytes. Co-IP results showed that LvHSSP could interact with VP26, the main envelope protein of WSSV, suggesting that LvHSSP might mediate WSSV adhesion and entry into host cells by binding to viral envelope protein. Meanwhile, the total hemocyte counts were significantly decreased after LvHSSP knockdown while increased after supplementing with recombinant LvHSSP protein, supporting the idea of hemocytes as the carrier for systemic dissemination of WSSV. This study reported a novel small protein in hemocytes, which modulated the viral infection in shrimp. Our results will enrich the knowledge of invertebrate innate immunity and provide a new field in the study of hemocyte function.
Antiviral Susceptibilities of Distinct Lineages of Influenza C and D Viruses

The emergence and spread of antiviral-resistant influenza viruses are of great concern. To minimize the public health risk, it is important to monitor antiviral susceptibilities of influenza viruses. Analyses of the antiviral susceptibilities of influenza A and B viruses have been conducted globally; however, those of influenza C and D viruses are limited. Here, we determined the susceptibilities of influenza C viruses representing all six lineages (C/Taylor, C/Yamagata, C/Sao Paulo, C/Aichi, C/Kanagawa, and C/Mississippi) and influenza D viruses representing four lineages (D/OK, D/660, D/Yama2016, and D/Yama2019) to RNA polymerase inhibitors (baloxavir and favipiravir) by using a focus reduction assay. All viruses tested were susceptible to both drugs. We then performed a genetic analysis to check for amino acid substitutions associated with baloxavir and favipiravir resistance and found that none of the viruses tested possessed these substitutions. Use of the focus reduction assay with the genotypic assay has proven valuable for monitoring the antiviral susceptibilities of influenza C and D viruses as well as influenza A and B viruses. Antiviral susceptibility monitoring of all influenza virus types should continue in order to assess the public health risks posed by these viruses.
How Different Types of Linguistic Information Impact Voice Perception: Evidence From the Language-Familiarity Effect

Previous studies have suggested the effect of linguistic information on voice perception (e.g., the language-familiarity effect [LFE]). However, it remains unclear which type of specific information in speech contributes to voice perception, including acoustic, phonological, lexical, and semantic information. It is also underexamined whether the roles of these different types of information are modulated by the experimental paradigm (speaker discrimination vs. speaker identification). In this study, we conducted two experiments to investigate these issues regarding LFEs. Experiment 1 examined the roles of acoustic and phonological information in speaker discrimination and identification with forward and time-reversed Mandarin and Indonesian sentences. Experiment 2 further identified the roles of phonological, lexical, and semantic information with forward, word-scrambled, and reconstructed (consisting of pseudo-Mandarin words) Mandarin and forward Indonesian sentences. For Mandarin-only participants, in Experiment 1, speaker discrimination was more accurate for forward than reversed sentences, but there was no LFE in either sentence. Speaker identification was also more accurate for forward than reversed sentences, whereas there was an LFE for forward sentences. In Experiment 2, speaker discrimination was better for word-scrambled than reconstructed Mandarin sentences. Speaker identification was more accurate for forward and word-scrambled Mandarin sentences but less accurate for Mandarin reconstructed and forward Indonesian sentences. In general, the pattern of the results for Indonesian learners was the same as that for Mandarin-only speakers. These results suggest that different kinds of information support speaker discrimination and identification in native and unfamiliar languages. The LFE in speaker identification depends on both phonological and lexical information.
Oil Adsorption Kinetics of Calcium Stearate-Coated Kapok Fibers

This study used a simple and efficient dipping method to prepare oleophilic calcium stearate-coated kapok fibers (CaSt2-KF) with improved hydrophobicity. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) confirmed the deposition of calcium stearate particles on the surface of the kapok fibers. This led to higher surface roughness and improved static water contact angle of 137.4°. The calcium stearate-coated kapok fibers exhibited comparable sorption capacities for kerosene, diesel, and palm oil. However, the highest sorption capacity of 59.69 g/g was observed for motor oil at static conditions. For motor oil in water, the coated fibers exhibited fast initial sorption and a 65% removal efficiency after 30 s. At equilibrium, CaSt2-KF attained a sorption capacity of 33.9 g/g and 92.5% removal efficiency for motor oil in water. The sorption kinetics of pure motor oil and motor oil in water follows the pseudo-second-order kinetic model, and the Elovich model further described chemisorption. Intraparticle diffusion and liquid film diffusion were both present, with the latter being the predominant diffusion mechanism during motor oil sorption.
Associations Between Cognitive Function and ACL Injury-Related Biomechanics: A Systematic Review

Context: Does lower baseline cognitive function predispose athletes to ACL injury risk, especially when performing unplanned or dual-task movements? Objective: To evaluate the association between cognitive function and biomechanics related to ACL injuries during cognitively challenging sports movements. Data Sources: PubMed (MEDLINE), Web of Science, Scopus, and SciELO databases were searched; additional hand searching was also conducted. Study Selection: The following inclusion criteria had to be met: participants completed (1) a neurocognitive test, (2) a cognitively challenging sport-related task involving lower limbs, and (3) a biomechanical analysis. The following criteria determined exclusion from the review: studies involving participants with (1) recent or current musculoskeletal injuries; (2) recent or current concussion; (3) ACL surgical reconstruction, reviews of the literature, commentary or opinion articles, and case studies. Study Design: Systematic review using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P) statement and registered at the International Prospective Register of Systematic Reviews (PROSPERO). Level of Evidence: Level 3. Data Extraction: Two of authors independently extracted data and assessed the methodological quality of the articles with the Downs and Black and ROBINS-I checklists, to assess methodological quality and risk of bias, respectively. Results: Six studies with different methodologies and confounding factors were included in this review. Of these 6 studies, 3 were ranked as high-quality, 3 demonstrated a low risk of bias, 2 a moderate risk, and 1 a severe risk. Five studies found a cognitive-motor relationship, with worse cognitive performance associated with increased injury risk, with 1 study reporting the opposite directionality for 1 variable. One study did not identify any interaction between cognitive function and biomechanical outcomes. Conclusion: Worse cognitive performance is associated with an increased injury risk profile during cognitively challenging movements.
The Anti-Tubercular Aminolipopeptide Trichoderin A Displays Selective Toxicity against Human Pancreatic Ductal Adenocarcinoma Cells Cultured under Glucose Starvation

Pancreatic ductal adenocarcinoma remains a highly debilitating condition with no effective disease-modifying interventions. In our search for natural products with promising anticancer activity, we identified the aminolipopeptide trichoderin A as a potential candidate. While it was initially isolated as an antitubercular peptide, we provide evidence that it is also selectively toxic against BxPC-3 and PANC-1 human pancreatic ductal adenocarcinoma cells cultured under glucose deprivation. This has critical implications for the pancreatic ductal adenocarcinoma, which is characterized by nutrient deprivation due to its hypovascularized network. We have also successfully simplified the trichoderin A peptide backbone, allowing greater accessibility to the peptide for further biological testing. In addition, we also conducted a preliminary investigation into the role of peptide lipidation at the N-terminus. This showed that analogues with longer fatty acyl chains exhibited superior cytotoxicity than those with shorter acyl chains. Further structural optimization of trichoderin A is anticipated to improve its biological activity, whilst ongoing mechanistic studies to elucidate its intracellular mechanism of action are conducted in parallel.
Guaiacol oxidation: theoretical insight into thermochemistry of radical processes involving methoxy group demethylation

Abstract Guaiacol (2-methoxyphenol) is naturally occurring phenolic compound essential in various research areas. Oxidative transformation of guaiacol can lead to the formation of various products, including 1,3-benzodioxole or ortho-quinone. Therefore, this study is focused on the investigation of the reaction enthalpies of experimentally observed guaiacol oxidation pathways in gas-phase, as well as in non-polar environment and aqueous solution. Corresponding Density Functional Theory (DFT) calculations were carried out using two hybrid functionals (M06-2X and B3LYP-D3). All reaction enthalpies, as well as Gibbs free energies, were also calculated using composite ab initio G4 method. M06-2X and G4 results show mutual agreement and the best accordance with available experimentally determined reaction enthalpies. Obtained Gibbs free reaction energies indicate that formation of ortho-quinone is thermodynamically preferred to formation of 1,3-benzodioxole at 298 K in studied environments. Moreover, all computational methods confirm that the reaction enthalpy of methoxy group demethylation, i.e. O–C bond dissociation enthalpy (BDE), is substantially lower in comparison to the enthalpy of hydrogen atom transfer from phenolic OH group. In the case of phenoxide anion of guaiacol, which can be formed in ionization supporting solvents, O–C BDE shows further significant decrease, exceeding 50 kJ mol−1, in comparison to parent molecule. HIGHLIGHTS Reaction enthalpies and Gibbs free energies of individual steps of guaiacol transformation to 1,3-benzodioxole or ortho-quinone are studied in three environments. M06-2X functional and composite ab initio G4 methods provide reliable O–H and O–C bond dissociation enthalpies. Dissociation enthalpy of methoxy group O–C bond is lower by ca. 100 kJ mol−1 in comparison to phenolic O–H bond. Phenoxide anion of guaiacol shows substantially lower O–C BDE than parent molecule.
New Algorithms for Ultra-relativistic Numerical Hydrodynamics

Two new transport algorithms for solving the one-dimensional relativistic hydrodynamic equations of motion are discussed. One of them, relativistic HLLE, is based on the HLLE upwind scheme, while the other is a relativistic adaptation of some kinds of flux corrected transport (FCT) algorithms. A comparison with other numerical methods used in relativistic hydrodynamics including fully implicit techniques is given. Emphasis is put on the dependence of the results on the relativistic gamma factor and on the adiabatic exponent entering the ideal gas equation of state. Future applications of the algorithms are particularly in the field of ultra-relativistic heavy ion collisions that require transport methods not critically dependent on the Lorentz gamma or the equation of state.
Males’ Lived Experience with Self-Perceived Pornography Addiction: A Qualitative Study of Problematic Porn Use

The positive impact of pornography use has been demonstrated; however, most research points towards problematic, compulsive, or excessive engagement with pornography and associated adverse effects on well-being. However, results remain inconclusive and qualitative research capturing perspectives of affected people is scarce. This phenomenological study aimed to explore the perspective and lived experience of males with a self-reported addiction to pornography. Semi-structured in-depth interviews with 13 males aged between 21 and 66 years from Australia and the USA were conducted. A thematic analysis of the transcripts was undertaken, resulting in the identification of four themes. The interviews explored the participants’ reasoning for determining themselves as porn addicts, investigated patterns of use, examined the perceived multifaceted impacts of pornography use, illustrated applied individual strategies to overcome the addiction, and proposed interventions helping to inform future recommendations. Experiences and perceptions of pornography addiction were consistently depicted as problematic and harmful. Most participants described an inability to stop their consumption despite experiencing adverse effects. Commonly reported was a gradual increase in the use of and consumption of new or more shocking content. Consumption of content was outlined as an escape or coping mechanism for negative emotions or boredom. Participants reported a variety of applied strategies to manage their addiction and suggested recommendations. Investigation into strategies for the identification of problematic pornography use, its conceptualization, associated health outcomes, and effective preventative and interventional strategies are required to provide academic consistency, support those negatively affected by pornography, and achieve increased public awareness of the issue.
Comparison of Transperitoneal and Retroperitoneal Robotic Partial Nephrectomy for Patients with Completely Lower Pole Renal Tumors

(1) Background: For completely lower pole renal tumors, we compared the perioperative outcomes of robotic partial nephrectomy via transperitoneal and retroperitoneal approaches. (2) Methods: Complete lower pole renal tumors were defined as tumors that received 1 point for the “L” element of the R.E. N.A. L. and located at the lower pole of kidney. After confirming consistency in baseline characteristics, oncological and functional benefits were compared. Pentafecta achievement was used to represent the perioperative optimal outcome, followed by multivariate analysis of factors associated with the lack of pentafecta achievement. (3) Results: Among 151 patients identified, 116 (77%) underwent robotic partial nephrectomy via a transperitoneal approach and 35 (23%) via a retroperitoneal approach. Patients undergoing transperitoneal robotic partial nephrectomy experienced more blood loss than those undergoing retroperitoneal robotic partial nephrectomy (50 mL vs. 40 mL, p = 0.015). No significant differences were identified for operative time (120 min vs. 120 min), ischemia time (19 min vs. 20 min), positive surgical margins (0.0% vs. 2.86%), postoperative rate of complication (12.07% vs. 5.71%). No significant differences were identified in pathologic variables, eGFR decline in postoperative 12-month (3.9% vs. 5.4%) functional follow-up. Multivariate cox analysis showed that tumor size (OR: 0.523; 95% CI: 0.371–0.736; p < 0.001) alone was independently correlated to the achievement of pentafecta. (4) Conclusions: For completely lower pole renal tumors, transperitoneal and retroperitoneal robotic partial nephrectomy provide similar outcomes. These two surgical approaches remain feasible options for these cases.
Simulated Microgravity Disrupts Nuclear Factor κB Signaling and Impairs Murine Dendritic Cell Phenotype and Function

During spaceflights, astronauts face different forms of stress (e.g., socio-environmental and gravity stresses) that impact physiological functions and particularly the immune system. In this context, little is known about the effect of such stress on dendritic cells (DCs). First, we showed that hypergravity, but not chronic ultra-mild stress, a socio-environmental stress, induced a less mature phenotype characterized by a decreased expression of MHCII and co-stimulatory molecules. Next, using the random positioning machine (RPM), we studied the direct effects of simulated microgravity on either splenic DCs or Flt-3L-differentiated bone marrow dendritic cells (BMDCs). Simulated microgravity was found to reduce the BM-conventional DC (cDC) and splenic cDC activation/maturation phenotype. Consistent with this, BMDCs displayed a decreased production of pro-inflammatory cytokines when exposed to microgravity compared to the normogravity condition. The induction of a more immature phenotype in microgravity than in control DCs correlated with an alteration of the NFκB signaling pathway. Since the DC phenotype is closely linked to their function, we studied the effects of microgravity on DCs and found that microgravity impaired their ability to induce naïve CD4 T cell survival, proliferation, and polarization. Thus, a deregulation of DC function is likely to induce immune deregulation, which could explain the reduced efficiency of astronauts’ immune response.
Comparative Proteomics and Genome-Wide Druggability Analyses Prioritized Promising Therapeutic Targets against Drug-Resistant Leishmania tropica

Leishmania tropica is a tropical parasite causing cutaneous leishmaniasis (CL) in humans. Leishmaniasis is a serious public health threat, affecting an estimated 350 million people in 98 countries. The global rise in antileishmanial drug resistance has triggered the need to explore novel therapeutic strategies against this parasite. In the present study, we utilized the recently available multidrug resistant L. tropica strain proteome data repository to identify alternative therapeutic drug targets based on comparative subtractive proteomic and druggability analyses. Additionally, small drug-like compounds were scanned against novel targets based on virtual screening and ADME profiling. The analysis unveiled 496 essential cellular proteins of L. tropica that were nonhomologous to the human proteome set. The druggability analyses prioritized nine parasite-specific druggable proteins essential for the parasite’s basic cellular survival, growth, and virulence. These prioritized proteins were identified to have appropriate binding pockets to anchor small drug-like compounds. Among these, UDPase and PCNA were prioritized as the top-ranked druggable proteins. The pharmacophore-based virtual screening and ADME profiling predicted MolPort-000-730-162 and MolPort-020-232-354 as the top hit drug-like compounds from the Pharmit resource to inhibit L. tropica UDPase and PCNA, respectively. The alternative drug targets and drug-like molecules predicted in the current study lay the groundwork for developing novel antileishmanial therapies.
Study on the Effect of Freeze–Thaw Action on the Electrical Conductivity and Sensing Properties of Graphene-Based Cement Composites

Graphene can effectively improve the mechanical and electrical properties of cement-based materials due to its excellent tensile strength, thermal conductivity and electrical conductivity. In this paper, the effects of freeze–thaw on the conductivity and sensing properties of graphene-based cement materials were investigated. After the preparation of graphene-based cement materials, they were subjected to different times of freeze–thaw action. The experiments were designed to analyze the influence of freeze–thaw on the electrical conductivity, humidity sensitivity, thermosensitivity and pressure sensitivity of graphene-based cement composites. The results show that the influence of freeze–thaw on the electrical conductivity of graphene is mainly manifested in the influence on the resistivity and the extension of the polarization time, and the influence on the percolation transition zone is small. After freeze–thaw, the polarization time of the specimen decreases with the increase of the relative water content. The temperature has a great influence on the polarization effect of graphene-based cement composites and the composites with graphene content of the zone B still show satisfactory pressure-sensitive property after freeze–thaw.
Environmental Pollution Liability Insurance Pricing and the Solvency of Insurance Companies in China: Based on the Black–Scholes Model

Environmental pollution liability insurance is becoming increasingly important for China to achieve its emission reduction targets. Insurance pricing is a crucial factor restricting the market share of environment pollution liability insurance, from the perspective of the Black–Scholes pricing model, which in turn has influenced the solvency of insurance companies in China. Firstly, this study analyzes the problems existing in compulsory liability insurance for environmental pollution in China. It proceeds with analyzing the price of compulsory environmental pollution liability insurance using the Black–Scholes pricing model, and derives a high premium insurance rate of 2.44%. Moreover, it performs a multivariate regression analysis using the asset and liability data, taken from the annual report, to identify three key factors affecting the solvency adequacy ratio, namely, capital debt ratio, reflecting the company asset structure; net interest rate on assets, reflecting the asset scale with actual solvency; and claim ratio, reflecting the business quality. Based on the results of regression analysis and robustness test for the China Insurance Clauses (CIC) company, People’s Insurance Company of China (PICC), and Asia-Pacific Property & Casualty Insurance (API) company, it is shown that the effect of total asset, total debt, capital debt ratio, claim ratio, and net interest rate on assets on the solvency adequacy ratio is significant, with respect to the size of the coefficients. Based on the Black–Scholes pricing model found in the previous cycle of liability insurance, and keeping in view the existing problems of environmental pollution liability insurance expenditure, this paper presents suggestions that are conducive to improving the solvency of insurance companies in China.