Miracle-dz/UniFAR-FTUs huggingface dataset:
Microrna-125a-5p regulates cancer cell proliferation and migration through naiF1 in prostate carcinoma

Background: We investigated the functional roles of microRNA-125a-5p in regulating human prostate carcinoma. Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was conducted to evaluate the gene expression levels of miR-125a-5p in eight prostate cancer cell lines and nine biopsy specimens from patients with prostate cancer. miR-125a-5p was geneti-cally knocked down in prostate cancer cell lines, DU145 and VCaP cells by lentiviral transduction. The effects of miR-125a-5p downregulation on prostate cancer cell proliferation and migration were evaluated by MTT assay and transwell assay, respectively. Direct regulation of miR-125a-5p on its downstream targets, NAIF1, and apoptotic gene caspase-3 were evaluated through dual-luciferase reporter assay, qRT-PCR, and Western blot, respectively. NAIF1 was then ectopically overexpressed in DU145 and VCaP cells to modulate prostate cancer cell proliferation and migration. Finally, the effects of miR-125a-5p downregulation or NAIF1 overexpression on the growth of in vivo prostate cancer xenograft were evaluated. Results: miR-125a-5p was upregulated in prostate cancer cell lines and human prostate carcinomas. Lentivirus induced miR-125a-5p downregulation in DU145 and VCaP cells inhibited prostate cancer cell proliferation or migration. NAIF1 was the direct target of miR-125a-5p, as both gene and protein expression levels of NAIF1, as well as caspase-3 were upregulated by miR-125a-5p. Forced overexpression of NAIF1 had similar antitumor effects as miR-125a-5p downregulation on prostate cancer cell proliferation and migration. In vivo prostate xenograft assay confirmed the tumor-suppressive effect of miR-125a-5p downregulation or NAIF1 overexpression. Conclusion: miR-125a-5p regulates prostate cancer cell proliferation and migration through NAIF1.
Progress on the commissioning of the Iseult 11.7T whole-body MRI: first images

The Iseult project started in 2001 and is a collaborative effort involving CEA and the University of Freiburg in academics, Guerbet, Siemens Healthineers and Bruker Biospin as industrial partners. One central aspect of the project has been the design by CEA of a whole-body magnet of 11.7T field strength with a 90-cm wide bore. After nearly 20 years of research and development, prototyping, integration and tests, first images have been acquired with this unique MRI scanner. Some key validation results are presented here.
Environmental Assessment of the Agricultural Sector

One of the main problems recognized in sustainable development goals and sustainable agricultural objectives is Climate change. Farming contributes significantly to the overall Greenhouse gases (GHG) in the atmosphere, which is approximately 10-12 percent of total GHG emissions, but when taking in consideration also land-use change, including deforestation driven by agricultural expansion for food, fiber and fuel the number rises to approximately 30 percent (Smith et. al., 2007). There are two distinct methodological approaches for environmental impact assessment; Life Cycle Assessment (a bottom up approach) and Input-Output Analysis (a top down approach). The two methodologies differ significantly but there is not an immediate choice between them if the scope of the study is on a sectorial level. Instead, as an alternative, hybrid approaches which combine these two approaches have emerged. The aim of this study is to analyze in a greater detail the agricultural sectors contribution to Climate change caused by the consumption of food products. Hence, to identify the food products that have the greatest impact through their life cycle, identifying their hotspots and evaluating the mitigation possibilities for the same. At the same time evaluating methodological possibilities and models to be applied for this purpose both on a EU level and on a country level (Italy).
Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress

Climate change jeopardizes soybean production by declining seed yield and quality. In this review, the morphophysiological alterations of soybean in response to abiotic stress are summarized, followed by illustrations of cellular metabolisms and regulatory mechanisms to organellar stress based on subcellular proteomics. This highlights the communications associated with reactive oxygen species scavenging, molecular chaperones, and phytohormone signals among subcellular compartments. Given the complexity of climate change and the limitations of plants in coping with multiple abiotic stresses, a generic response to environmental constraints is proposed between calcium and abscisic acid signals in subcellular organelles. This review summarizes the findings of subcellular proteomics in stressed soybean and discusses the future prospects of subcellular proteomics for promoting the improvement of climate-tolerant crops.
Analysis of the disease burden of cardiomyopathy in children aged 0–14 years in China from 1990 to 2019

Objectives To assess the disease burden and changing trend of cardiomyopathy in children aged 0–14 years in China from 1990 to 2019. Methods This study was based on the Global Burden of Disease Study 2019; the age-specific prevalence rate, mortality rate and disability-adjusted life year (DALY) rate were used for analysis. Estimated annual percentage change (EAPC) in burden rate and its 95% confidence interval were calculated. The data of China were compared with the global average level. Results In 2019, the numbers of prevalence, deaths, and DALYs of cardiomyopathy in children aged 0–14 years in China were 4,493 [95% uncertainty interval (UI): 2687 ~ 6,838], 434 (95%UI: 337 ~ 565) and 37,522 (95%UI: 29,321 ~ 48,891), with declining amplitudes of 16.32, 70.56, and 70.74%, compared with 1990, respectively. In 2019, the prevalence rate of cardiomyopathy in Chinese children aged 0–14 years was 2.00/100,000 (95%UI: 1.2/100,000 ~ 3.04/100,000), higher than 1990 [1.66/100,000 (95%UI:1.00/100,000 ~ 2.53/100,000)]; mortality rate was 0.19/100,000 (95%UI: 0.15/100,000 ~ 0.25/100,000), significantly lower than 1990 [0.46/100,000 (95%UI: 0.25/100,000 ~ 0.95/100,000)]; DALY rate was 16.69/100,000 (95%UI: 13.04/100,000 ~ 21.75/100,000), also significantly lower than 1990 [39.71/100,000 (95%UI: 22.06/100,000 ~ 82.8/100,000)]. All burden rates of cardiomyopathy in Chinese children aged 0–14 years old were all lower than the global averages of 2019; the burden rates of male children were higher than female children. In all calendar years from 1990 to 2019, the mortality and DALY rates of children younger than 1-year-old were significantly higher than in the other age groups of 0–14 years old. From 1990 to 2019, the prevalence rate of cardiomyopathy aged 0–14 years old gradually increased, with EAPC of 0.82 (95%CI: 0.71–0.93); mortality rate and DALY rate decreased [EAPC = −2.32 (95%CI: −2.59 to −2.05)]. Conclusion From 1990 to 2019, the disease burden of cardiomyopathy in children of China aged 0–14 years was heterogeneous; the burden of male children was higher than females; and the burden of cardiomyopathy in children younger than 1 year old needs more attention.
Respiratory Muscle Function in Older Adults with Chronic Respiratory Diseases after Pulmonary Rehabilitation in Subterranean Salt Chambers

Training the respiratory muscles is a crucial aspect of pulmonary rehabilitation. The purpose of this study was to assess the function of respiratory muscles in older adults both before and after a period of pulmonary rehabilitation and treatment stay within the underground chambers of a salt mine. A total of 50 patients aged 65 years and older with chronic respiratory conditions was enrolled in the study. These participants underwent a 3-week subterranean pulmonary rehabilitation (PR) program in the “Wieliczka” Salt Mine. Levels of sniff nasal inspiratory pressure (SNIP), maximum inspiratory pressure (MIP), and maximum expiratory pressure (MEP) were measured using the MicroRPM both before and after the outpatient PR program conducted 135 m underground. A total of 44 patients with a mean age of 68.8 ± 2.9 years who completed the PR program and tests were included in the analysis. The average changes in the parameters of pulmonary function before and after the PR were: MIP 8.8 cmH2O, MEP 7.1 cmH2O, and SNIP 11.2 cmH2O (for p < 0.05). For patients older than 70 years, beneficial changes were only observed for MEP, which increased by 9.3 cmH2O (for p < 0.05). Speleotherapy combined with pulmonary rehabilitation improves respiratory muscle function in older adults with chronic respiratory diseases, mainly in terms of MEP. Therefore, a greater emphasis on inspiratory muscle training in the rehabilitation program should be considered.
Predictors of treatment dropout in patients with posttraumatic stress disorder due to childhood abuse

Background Knowledge about patient characteristics predicting treatment dropout for post-traumatic stress disorder (PTSD) is scarce, whereas more understanding about this topic may give direction to address this important issue. Method Data were obtained from a randomized controlled trial in which a phase-based treatment condition (Eye Movement Desensitization and Reprocessing [EMDR] therapy preceded by Skills Training in Affect and Interpersonal Regulation [STAIR]; n = 57) was compared with a direct trauma-focused treatment (EMDR therapy only; n = 64) in people with a PTSD due to childhood abuse. All pre-treatment variables included in the trial were examined as possible predictors for dropout using machine learning techniques. Results For the dropout prediction, a model was developed using Elastic Net Regularization. The ENR model correctly predicted dropout in 81.6% of all individuals. Males, with a low education level, suicidal thoughts, problems in emotion regulation, high levels of general psychopathology and not using benzodiazepine medication at screening proved to have higher scores on dropout. Conclusion Our results provide directions for the development of future programs in addition to PTSD treatment or for the adaptation of current treatments, aiming to reduce treatment dropout among patients with PTSD due to childhood abuse.
Intense and Superflat White Laser with 700-nm 3-dB Bandwidth and 1-mJ Pulse Energy Enabling Single-Shot Subpicosecond Pulse Laser Spectroscopy

An optical spectrometer is a basic spectral instrument that probes microscopic physical and chemical properties of macroscopic objects but generally suffers from difficulty in broadband time-resolved measurement. In this work, we report the creation of ultrabroadband white-light laser with a 3-dB bandwidth covering 385 to 1,080 nm, pulse energy of 1.07 mJ, and pulse duration of several hundred femtoseconds by passing 3-mJ pulse energy, 50-fs pulse duration Ti:Sapphire pulse laser through a cascaded fused silica plate and chirped periodically poled lithium niobate crystal. We utilize this unprecedented superflat, ultrabroadband, and intense femtosecond laser light source to build a single-shot (i.e., single-pulse) subpicosecond pulse laser ultraviolet–visible–near-infrared spectrometer and successfully measure various atomic and molecular absorption spectra. The single-shot ultrafast spectrometer may open up a frontier to monitor simultaneously the ultrafast dynamics of multiple physical and chemical processes in various microscopic systems.
Rutin: Family Farming Products’ Extraction Sources, Industrial Applications and Current Trends in Biological Activity Protection

In vitro and in vivo studies have demonstrated the bioactivity of rutin, a dietary flavonol naturally found in several plant species. Despite widespread knowledge of its numerous health benefits, such as anti-inflammatory, antidiabetic, hepatoprotective and cardiovascular effects, industrial use of rutin is still limited due to its low solubility in aqueous media, the characteristic bitter and astringent taste of phenolic compounds and its susceptibility to degradation during processing. To expand its applications and preserve its biological activity, novel encapsulation systems have been developed. This review presents updated research on the extraction sources and methodologies of rutin from fruit and vegetable products commonly found in a regular diet and grown using family farming approaches. Additionally, this review covers quantitative analysis techniques, encapsulation methods utilizing nanoparticles, colloidal and heterodisperse systems, as well as industrial applications of rutin.
Areal Precipitation and Temperature in the Swedish Mountains: An Evaluation from a Hydrological Perspective

This paper presents an evaluation of three different methods for estimation of areal precipitation and temperature, with special emphasis on their applicability for runoff modelling in the Swedish mountains. All three methods estimate the areal values as a weighted mean of the observations at nearby meteorological stations. The weights are determined by: 1) a manual subjective selection of the most representative stations 3) inverse square distance weighting 4) optimal interpolation The methods were tested in an area with complex topography and precipitation gradients. The evaluation included comparison of areal estimates, verification against point observations and the water balance equation, and sensitivity analyses with respect to method parameters and network changes. The evaluation showed that for simple runoff modelling the subjective and optimal interpolation methods performed equally well, and considerably better than inverse-distance weighting. The evaluation also showed that none of the methods correctly described the spatial variation in precipitation and temperature in the investigated region. They are thus not directly applicable for non-routine modelling applications where the estimation of runoff is not the sole objective. All methods proved to be sensitive to the selection of parameter values, which pointed to possible improvements of the estimates. The optimal interpolation method seemed to be the least sensitive to changes in the meteorological network.
Motion Capture Technologies for Ergonomics: A Systematic Literature Review

Muscular skeletal disorder is a difficult challenge faced by the working population. Motion capture (MoCap) is used for recording the movement of people for clinical, ergonomic and rehabilitation solutions. However, knowledge barriers about these MoCap systems have made them difficult to use for many people. Despite this, no state-of-the-art literature review on MoCap systems for human clinical, rehabilitation and ergonomic analysis has been conducted. A medical diagnosis using AI applies machine learning algorithms and motion capture technologies to analyze patient data, enhancing diagnostic accuracy, enabling early disease detection and facilitating personalized treatment plans. It revolutionizes healthcare by harnessing the power of data-driven insights for improved patient outcomes and efficient clinical decision-making. The current review aimed to investigate: (i) the most used MoCap systems for clinical use, ergonomics and rehabilitation, (ii) their application and (iii) the target population. We used preferred reporting items for systematic reviews and meta-analysis guidelines for the review. Google Scholar, PubMed, Scopus and Web of Science were used to search for relevant published articles. The articles obtained were scrutinized by reading the abstracts and titles to determine their inclusion eligibility. Accordingly, articles with insufficient or irrelevant information were excluded from the screening. The search included studies published between 2013 and 2023 (including additional criteria). A total of 40 articles were eligible for review. The selected articles were further categorized in terms of the types of MoCap used, their application and the domain of the experiments. This review will serve as a guide for researchers and organizational management.
Information Hiding: 6th International Workshop, IH 2004, Toronto, Canada, May 23-25, 2004, Revised Selected Papers

Session 1 - Digital Media Watermarking Session Chair: Lisa Marvel (University of Delaware). - An Implementation of, and Attacks on, Zero-Knowledge Watermarking. - On the Possibility of Non-invertible Watermarking Schemes. - Reversing Global and Local Geometrical Distortions in Image Watermarking. - On Achievable Regions of Public Multiple-Access Gaussian Watermarking Systems. - Fixed-Distortion Orthogonal Dirty Paper Coding for Perceptual Still Image Watermarking. - Session 2 - Steganalysis Session Chair: Mauro Barni (University of Siena). - Feature-Based Steganalysis for JPEG Images and Its Implications for Future Design of Steganographic Schemes. - Exploiting Preserved Statistics for Steganalysis. - Improved Detection of LSB Steganography in Grayscale Images. - An Improved Sample Pairs Method for Detection of LSB Embedding. - Session 3 - Forensic Applications Session Chair: Scott Craver (Princeton University). - Statistical Tools for Digital Forensics. - Relative Generic Computational Forensic Techniques. - Session 4 - Steganography Session Chair: Andreas Westfeld (Dresden University of Technology). - Syntax and Semantics-Preserving Application-Layer Protocol Steganography. - A Method of Linguistic Steganography Based on Collocationally-Verified Synonymy. - Session 5 - Software Watermarking Session Chair: John McHugh (SEI/CERT). - Graph Theoretic Software Watermarks: Implementation, Analysis, and Attacks. - Threading Software Watermarks. - Soft IP Protection: Watermarking HDL Codes. - Session 6 - Security and Privacy Session Chair: Ross Anderson (University of Cambridge). - An Asymmetric Security Mechanism for Navigation Signals. - Analysis of COT-based Fingerprinting Schemes: New Approach to Design Practical and Secure Fingerprinting Scheme. - Empirical and Theoretical Evaluation of Active Probing Attacks and Their Countermeasures. - Optimization and Evaluation of Randomized c-Secure CRT Code Defined on Polynomial Ring. - Session 7 - Anonymity Session Chair: Andreas Pfitzmann (Dresden University of Technology). - Statistical Disclosure or Intersection Attacks on Anonymity Systems. - Reasoning About the Anonymity Provided by Pool Mixes That Generate Dummy Traffic. - The Hitting Set Attack on Anonymity Protocols. - Session 8 - Data Hiding in Unusual Content Session Chair: Christian Collberg (University of Arizona). - Information Hiding in Finite State Machine. - Covert Channels for Collusion in Online Computer Games.
Dinamika Internet, Media Sosial, dan Politik di Era Kontemporer: Tinjauan Relasi Negara-Masyarakat

Era kontemporer dalam demokrasi elektoral menunjukkan pertarungan politik yang sangat kompetitif, keras, dan melibatkan berbagai isu sebagai alat untuk mendapatkan kekuasaan. Kandidat yang berpartisipasi dalam pemilihan umum, baik itu pemilihan legislatif, presiden, maupun kepala daerah, tidak dapat mengandalkan saluran komunikasi politik konvensional saja. Kondisi ini menandapat perlu adanya kajian yang menjelaskan mengenai internet, media sosial, dan politik sebagai bagian integral dalam realitas sosial kontemporer. Tujuan dari penelitian ini ialah mengkaji dinamika internet, media sosial, dan politik di era kontemprer di masyarakat sebagai bagian penting bagi perkembangan peradaban suatu bangsa. Penelitian ini menggunakan pendekatan kualitatif dengan studi kepustakaan yang berbasis pada temuan-temuan penelitian terdahulu, kemudian dikonstruk untuk mendapatkan penjelasan mengenai realitas kontemporer terkiat internet, media sosial, dan politik. Hasil telaah menunjukkan bahwa kegiatan dalam kegiatan politik kontemporer para aktor perlu mengadopsi pendekatan media baru dengan segala karakteristiknya, yang memungkinkan interaksi lebih intensif. Kandidat juga harus menyadari bahwa media baru memiliki sisi-sisi lemah yang tidak selalu menguntungkan dalam proses pembentukan citra mereka di ruang publik. Media baru merupakan generasi ketiga dalam komunikasi politik, di mana siapa pun dapat menjadi produsen dan konsumen informasi. Pola interaksi yang bersifat resiprokal dan personal, meskipun seringkali artifisial, menjadi tantangan bagi kandidat dalam memposisikan diri di tengah informasi yang beragam dan cepat. Demokrasi siber, yang memfasilitasi identitas pribadi dan publik, seringkali mengalami pergeseran peran yang kompleks.
Porcine Germ Cells Phenotype during Embryonic and Adult Development

Simple Summary The lineage of germ cells (GCs) ensures the perpetuation and diversification of genetic information across generations in most vertebral organisms. As part of their development into functional gametes, PGCs undergo extensive genetic and epigenetic modifications during their specification and development. We aimed to understand the dynamics of pluripotent, germline, and epigenetic markers of porcine PGCs (pPGCs) during the gestational and adult periods in both genders. The results demonstrated morphological differences between embryonic/fetal ages and genders in the expression of pluripotency and germinal markers. Additionally, different patterns of epigenetic markers were found at these embryonic/fetal ages. These findings are significant to the field of germ cells because they exhibit the progression of a variety of markers during pPGCs, and they may allow their use in applied science such as reproductive and regenerative medicine. Abstract Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro.
Influences of Psychomotor Behaviors on Learning Swimming Styles in 6–9-Year-Old Children

The aim of this study was to identify the existence of some relationships between certain psychomotor behaviors, which we consider specific to swimming, and learning to execute the technique of some swimming styles (front crawl and backstroke). The study was carried out for 10 months and included 76 children (40 boys and 36 girls) aged between 6 and 9 years who practice recreational swimming in a city in Romania. Several tools were used: the Tapping test for manual dexterity, the Goodenough test for body schema, the Flamingo test for static balance, and the horizontal buoyancy test for body balance on the water. The results indicated better ratings on all psychomotor behaviors analyzed according to gender (in favor of girls compared to boys). The levels of all analyzed psychomotor behaviors have a direct relationship to the subjects’ age. Also, we identified moderate positive correlations for manual dexterity (rs = 0.63 in the front crawl style; rs = 0.57 in the backstroke style) and strong correlations for body schema, static balance and buoyancy, coordination with the learning of the two swimming styles (r or rs between 0.77 and 0.85). In conclusion, psychomotor behaviors can be predictors for learning swimming styles.