Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism

Purpose: This study was designed to evaluate the pharmacological mechanisms of puerarin against oliguria in acute alcoholism via network pharmacology analysis combined with experimental verification. Methods: First, this study established an acute alcoholism rat model, compared the changes in urine volume in each group, and observed the therapeutic effect of puerarin by H&E staining, biochemical, RT-qPCR, and immunohistochemical analyses. Second, puerarin-related targets were searched in TCMS, PubChem, CNKI, Wanfang, PubMed, and GeenMedical Academic databases. Also, potential disease targets were obtained from the GeneCards, MalaCards, and NCBI-gene databases and genes with puerarin target gene intersections were screened out. The interaction network for co-predicted targets was obtained using the STRING database, and the core targets were imported into Cytoscape for visualization using DAVID Bioinformatics Resources 6.8. The essential genes were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway enrichment analyses to predict related biological processes and significant signaling pathways. Finally, molecular docking was used to examine the interaction of puerarin with key targets, and the core targets were validated further by RT-qPCR and Western blotting. Results: Compared to the model group, the urine volume of the rats was significantly increased after puerarin treatment, and the levels of anti-diuretic hormone (ADH) and aquaporin 2 (AQP2) expression were decreased. Searching the intersection of puerarin and acute alcoholism targets yielded 214 potential targets, 837 biological processes, and 185 signaling pathways involved. The molecular docking results indicated a good affinity between puerarin and key targets (cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response element-binding protein (CREB), and c-Fos). RT-qPCR and Western blotting further verified that puerarin could down-regulate the expression of cAMP/PKA/CREB/c-Fos. Conclusion: This study identified the potential targets of puerarin against oliguria in rats with acute alcoholism using network pharmacology and animal experiments. The mechanism may be closely related to the cAMP signaling pathway.
Functions and strategies for enhancing zinc availability in plants for sustainable agriculture

Zinc (Zn), which is regarded as a crucial micronutrient for plants, and is considered to be a vital micronutrient for plants. Zn has a significant role in the biochemistry and metabolism of plants owing to its significance and toxicity for biological systems at specific Zn concentrations, i.e., insufficient or harmful above the optimal range. It contributes to several cellular and physiological activities of plants and promotes plant growth, development, and yield. Zn is an important structural, enzymatic, and regulatory component of many proteins and enzymes. Consequently, it is essential to understand the interplay and chemistry of Zn in soil, its absorption, transport, and the response of plants to Zn deficiency, as well as to develop sustainable strategies for Zn deficiency in plants. Zn deficiency appears to be a widespread and prevalent issue in crops across the world, resulting in severe production losses that compromise nutritional quality. Considering this, enhancing Zn usage efficiency is the most effective strategy, which entails improving the architecture of the root system, absorption of Zn complexes by organic acids, and Zn uptake and translocation mechanisms in plants. Here, we provide an overview of various biotechnological techniques to improve Zn utilization efficiency and ensure the quality of crop. In light of the current status, an effort has been made to further dissect the absorption, transport, assimilation, function, deficiency, and toxicity symptoms caused by Zn in plants. As a result, we have described the potential information on diverse solutions, such as root structure alteration, the use of biostimulators, and nanomaterials, that may be used efficiently for Zn uptake, thereby assuring sustainable agriculture.
The impact of environmental information disclosure on enterprises’ green preference of outbound investment: Evidence from China

China is accelerating green and low-carbon transformation and promoting high-quality economic development. To effectively contribute to the improvement of global environmental quality and enhance enterprises’ awareness of environmental responsibility in the process of outbound investment, China strongly advocates that enterprises should comply with the environmental protection laws and regulations of host countries and try to strengthen local environmental protection as investing abroad. However, inadequate attention has been paid to the factors influencing green preferences in corporate outbound investment. Therefore, using a sample of Chinese A-share listed companies from 2008 to 2019, this paper aims to empirically analyze whether the quality of environmental information disclosure (QEID) affects enterprises’ green preference of outbound investment (OIGP), and the influence mechanism of QEID on the enterprises’ OIGP by analyzing the mediating effects of green technology innovation and media attention. It is found that QEID significantly promotes corporate OIGP. In terms of influence mechanism, QEID promotes firms’ OIGP by restraining media attention, while the mediating role of green technology innovation in the effect of QEID on firms’ OIGP is manifested as the masking effect. In addition, it is found that these influence mechanisms are different among enterprises with different property rights and different life cycle stages.
On the maximum accretion luminosity of magnetized neutron stars: connecting X-ray pulsars and ultraluminous X-ray sources

We study properties of luminous X-ray pulsars using a simplified model of the accretion column. The maximal possible luminosity is calculated as a function of the neutron star (NS) magnetic field and spin period. It is shown that the luminosity can reach values of the order of $10^{40}\,{\rm erg/s}$ for the magnetar-like magnetic field ($B\gtrsim 10^{14}\,{\rm G}$) and long spin periods ($P\gtrsim 1.5\,{\rm s}$). The relative narrowness of an area of feasible NS parameters which are able to provide higher luminosities leads to the conclusion that $L\simeq 10^{40}\,\,{\rm erg/s}$ is a good estimate for the limiting accretion luminosity of a NS. Because this luminosity coincides with the cut-off observed in the high mass X-ray binaries luminosity function which otherwise does not show any features at lower luminosities, we can conclude that a substantial part of ultra-luminous X-ray sources are accreting neutron stars in binary systems.
Counterintuitive consequences of COVID-19 on healthcare workers: A meta-analysis of the relationship between work engagement and job satisfaction

Background Studies conducted in the health sector have determined a positive relationship between job satisfaction and work engagement. However, this paper reveals that this relationship turns into a negative or non-significant relationship during the COVID-19 pandemic. We explore the reasons for inconsistency in research findings in this critical period through a meta-analysis. Methods This study was conducted according to the PRISMA guidelines and PICO framework. Online databases including Web of Science, Scopus, PubMed, ProQuest, Google Scholar, and additional records from other databases were searched without any time limitation, and all studies published in English that reported the correlation between work engagement and job satisfaction were included in the analysis. In total, 36 individual correlation coefficients were synthesized. R statistical language was used to analyze the data. Result A total of 36 studies with a sample size of 16,087 were synthesized. The overall effect size was found as r = 0.57 (95% CI [0.50–0.64]). While the moderating effect of national culture was not statistically significant, presence of COVID-19 as the significant moderator explained 37.08% of effect size heterogeneity. Such that the presence of COVID-19 has transformed the positive relationship between work engagement and job satisfaction into a negative but statistically non-significant relationship. Conclusion This study empirically challenges the existing assumptions about the positive link between work engagement and job satisfaction. The results of the research can be a guide for managers and policymakers. Specifically, based on these results, different mechanisms can be put in place to support work engagement and, in turn, job satisfaction in the COVID-19 process.
Experimental study of camptothecin combined with drug-eluting bead transarterial chemoembolization in the rabbit VX2 liver tumor model

Drug-eluting bead transarterial chemoembolization (DEB-TACE) has been widely used in the treatment of liver cancer; however, the utilization rate of chemotherapeutic drugs after embolization is low. Chemotherapy resistance mediated by high nuclear factor E2-related factor 2 (NRF2) expression limits DEB-TACE efficacy. Camptothecin (CPT), an NRF2 inhibitor, exerts chemosensitizing effects. We designed a controlled experiment to determine the efficacy and feasibility of DEB-TACE combined with CPT for the treatment of rabbit VX2 hepatoma. DEB-TACE activated NRF2 expression in the tumor region. NRF2 activation could be inhibited by the combined use of CPT. After DEB-TACE alone, the tumor necrosis was incomplete, there were still highly active tumor residues, and the apparent diffusion coefficient (ADC) value, which was negatively correlated with tumor activity observed by magnetic resonance imaging, remained low. After DEB-TACE combined with CPT, the relative necrosis of the tumor was more complete, the ADC value was higher, and the ADC change was greater. The single application of CPT did not result in evident liver function and physical burden to the rabbits. The combined use of CPT and DEB-TACE did not significantly increase DEB-TACE imaging of liver function and body. In conclusion, CPT can also inhibit high NRF2 expression after DEB-TACE treatment. Combining CPT with DEB-TACE can improve the sensitivity of DEB-TACE in the treatment of VX2 tumors, improve the therapeutic effect, and has no evident toxic and side effects. This study explored the methods for enhancing the efficacy of DEB-TACE in liver cancer from a new perspective and performed model experiments, which provided a theoretical basis for future clinical treatment.
Investigating the Association Between Adolescent Internet Addiction and Parental Attitudes

Objectives: In this study, the association between parents’ attitudes and internet addiction among adolescent high school students was investigated through some sociodemographic variables. Methods: The research was carried out with 385 students studying in four high schools in western Turkey. Sociodemographic characteristics, parental attitude scale and internet addiction scale were used in the study. Descriptive statistics, logistic regression analysis and structural equation modeling analysis were applied. Results: The results of multivariate logistic regression analysis demonstrated that there was a significant relationship between the combined mean score obtained from the Parental Attitude Scale and the mean score obtained from the Internet Addiction Scale, and that authoritarian parenting attitude increases the risk of internet addiction [OR = 1.70 (95% CI: 1.33–2.18)]. In the structural equation modeling analysis, the model summary fit values were determined to be at a good level (χ2/df = 2.86, GFI = 0.97, RMSEA = 0.073) regarding the relationship between parental attitude and internet addiction. Conclusion: Adolescents’ internet addiction levels changes related to their parents’ democratic attitude evolve.
The role of calcium-calpain pathway in hyperthermia

Hyperthermia is a promising anticancer treatment modality. Heat stress stimulates proteolytic machineries to regulate cellular homeostasis. Calpain, an intracellular calcium (Ca2+)-dependent cysteine protease, is a modulator that governs various cellular functions. Hyperthermia induces an increase in cytosolic Ca2+ levels and triggers calpain activation. Contrastingly, pre-exposure of cells to mild hyperthermia induces thermotolerance due to the presence of cellular homeostatic processes such as heat shock response and autophagy. Recent studies suggest that calpain is a potential key molecule that links autophagy and apoptosis. In this review, we briefly introduce the regulation of intracellular Ca2+ homeostasis, basic features of calpains with their implications in cancer, immune responses, and the roles and cross-talk of calpains in cellular protection and cell death in hyperthermia.
Masquelet technique with radical debridement and alternative fixation in treatment of infected bone nonunion

Background Infected bone nonunion is the toughest problem in fracture-related infection, leading to high disability and recurrence. The aim of this study was to evaluate the effectiveness of the Masquelet technique with radical debridement and alternative fixation in the management of infected bone nonunion. Patients and Methods A retrospective study of prospectively collected data in two trauma centers was performed from 2016 to 2020. Patients diagnosed as infected bone nonunion were included in this study. The initial implant was removed and all patients received a two-stage Masquelet procedure with radical debridement and alternative fixation. The disappearance of inflammatory manifestations and regression of infection indicators (such as interleukin-6 (IL-6), C-reactive protein, white blood cell count) to the normal range were regarded as radical debridement. The alternative fixation depended on local soft tissue conditions. Results were evaluated according to clinical and radiographic assessment and patient satisfaction. Results A total of 23 patients were included in our study. Six of them received internal fixation, while the other 17 received external fixation. Of the 23 cases, 21 were successfully reconstructed without infection recurrence, except 2 reinfected cases. Mean full weight bearing time was 6.6 months follow-up post last surgery. Out of the 23, 20 cases had satisfactory functional outcomes without additional bone or soft tissue comorbidities. Discrepancies in leg length and joint stiffness were observed in three cases and marked as unsatisfied results. Conclusions Infected bone nonunion can be successfully managed using the Masquelet technique under radical debridement combined with an alternative fixation method.
Computational tools to study RNA-protein complexes

RNA is the key player in many cellular processes such as signal transduction, replication, transport, cell division, transcription, and translation. These diverse functions are accomplished through interactions of RNA with proteins. However, protein–RNA interactions are still poorly derstood in contrast to protein–protein and protein–DNA interactions. This knowledge gap can be attributed to the limited availability of protein-RNA structures along with the experimental difficulties in studying these complexes. Recent progress in computational resources has expanded the number of tools available for studying protein-RNA interactions at various molecular levels. These include tools for predicting interacting residues from primary sequences, modelling of protein-RNA complexes, predicting hotspots in these complexes and insights into derstanding in the dynamics of their interactions. Each of these tools has its strengths and limitations, which makes it significant to select an optimal approach for the question of interest. Here we present a mini review of computational tools to study different aspects of protein-RNA interactions, with focus on overall application, development of the field and the future perspectives.
Case report: Senescence as mechanism of resistance to Pembrolizumab in a Lymphoma patient who failed CD19-Targeted CAR-T cell therapy

Background T cells engineered to target CD19 antigen on neoplastic B cells represent the most striking example of CAR-T cell therapy. The success rate of this therapy is affected by several limitations: target antigen loss, and/or acquisition of a senescent/exhausted phenotype by CAR and non-CAR T cells. Case presentation We report on a patient affected by refractory Diffuse Large B-cell Lymphoma who was resistant to CAR T-cell therapy and to two cycles post CAR-T of pembrolizumab (PBZ) due to the evolution into a B-cell Hodgkin-like lymphoma. Owing to the CD30 expression and the Hodgkin-like phenotype, the patient was ultimately treated with Brentuximab-Vedotin and finally underwent remission. Upon PBZ treatment, 100% of circulating CAR-T+ cells showed a persistent CD8+ senescent/exhausted phenotype, while an increase in the percentage of senescent cells was found in the non-CAR CD8+ T cells compartment. Conclusions PBZ is not able to reinvigorate exhausted CAR+ T cells and to confer durable clinical response. We hypothesize that the phenomenon is due to the senescent phenotype of CAR+ T cells, which did not allow PBZ-induced reactivation and proliferative rescue. The phenomenon, together with the loss of CAR-T target CD19 and the shift of non-CAR CD8+ T cells towards a senescent phenotype likely contributed to set up an immune landscape with poor antitumor capacity.
ELECTROMAGNETIC TRANSIENTS POWERED BY NUCLEAR DECAY IN THE TIDAL TAILS OF COALESCING COMPACT BINARIES

We investigate the possibility that long tidal tails formed during compact object mergers may produce optical transients powered by the decay of freshly synthesized r-process material. Precise modeling of the merger dynamics allows for a realistic determination of the thermodynamic conditions in the ejected debris. We combine hydrodynamic and full nuclear network calculations to determine the resultant r-process abundances and the heating of the material by their decays. The subsequent homologous structure is mapped into a radiative transfer code to synthesize emergent model light curves and determine how their properties (variability and color evolution) depend on the mass ratio and orientation of the merging binary. The radiation emanating from the ejected debris, though less spectacular than a typical supernova, should be observable in transient surveys and we estimate the associated detection rates. We find that it is unlikely that photometry alone will be able to distinguish between different binary mass ratios and the nature of the compact objects, emphasizing the need for spectroscopic follow-up of these events. The case for (or against) compact object mergers as the progenitors of short gamma-ray bursts can be tested if such electromagnetic transients are detected (or not) in coincidence with some bursts, although they may be obscured by on-axis afterglows.
Characterization of Mycobacterium salfingeri sp. nov.: A novel nontuberculous mycobacteria isolated from a human wound infection

Nontuberculous mycobacteria (NTM) are environmental bacteria commonly found in soil and water in almost every part of the world. While usually non-pathogenic, they can cause acute respiratory and cutaneous infections under certain circumstances or in patients with underlying medical conditions. Contrary to members of the Mycobacterium tuberculosis complex, documented human-to-human transmissions of NTM have been rarely reported and most cases result from direct environmental exposure. Here we describe the identification of a new NTM species isolated from a hand laceration of a New York State patient after a fall. This new NTM forms rough, orange pigmented colonies and is naturally resistant to doxycycline and tobramycin. Whole genome analysis reveal no close relatives present in public databases, and our findings are in accordance with the recognition of a new taxonomic species of NTM. We propose the name Mycobacterium salfingeri sp. nov. for this new NTM representative. The type strain is 20-157661T (DSM = 113368T, BCCM = ITM 501207T).
Invariant Transformations, Conservation Laws, and Energy - Momentum

A printer head for use in an impact printer of the dotmatrix type utilizes a stack of pivoted thin blades, each having a printing tip at one end thereof. A pancake coil is attached to each blade for initiating selective independent movement thereof within a common, externally-produced magnetic field to efficiently convert electrical print signals to kinetic energy in each printing tip of a vertical array thereof thereby facilitating printing of symbols, characters and other indicia on underlying media with high resolution. The single magnetic-field-producing means interacts with all pancake coils of the stack of printer blades to facilitate close spacing of the printing tips for superior character printing. Resilient members are integrally formed in each blade to support the moving structure with negligible loss, thereby increasing the printing speed of the stacked blade head.
Combining robust level extraction and unsupervised adaptive classification for high-accuracy fNIRS-BCI: An evidence on single-trial differentiation between mentally arithmetic- and singing-tasks

Functional near-infrared spectroscopy (fNIRS) is a safe and non-invasive optical imaging technique that is being increasingly used in brain-computer interfaces (BCIs) to recognize mental tasks. Unlike electroencephalography (EEG) which directly measures neural activation, fNIRS signals reflect neurovascular-coupling inducing hemodynamic response that can be slow in time and varying in the pattern. The established classifiers extend the EEG-ones by mostly employing the feature based supervised models such as the support vector machine (SVM) and linear discriminant analysis (LDA), and fail to timely characterize the level-sensitive hemodynamic pattern. A dedicated classifier is desired for intentional activity recognition of fNIRS-BCI, including the adaptive acquisition of response relevant features and accurate discrimination of implied ideas. To this end, we herein propose a specifically-designed joint adaptive classification method that combines a Kalman filtering (KF) for robust level extraction and an adaptive Gaussian mixture model (a-GMM) for enhanced pattern recognition. The simulative investigations and paradigm experiments have shown that the proposed KF/a-GMM classification method can effectively track the random variations of task-evoked brain activation patterns, and improve the accuracy of single-trial classification task of mental arithmetic vs. mental singing, as compared to the conventional methods, e.g., those that employ combinations of the band-pass filtering (BPF) based feature extractors (mean, slope, and variance, etc.) and the classical recognizers (GMM, SVM, and LDA). The proposed approach paves a promising way for developing the real-time fNIRS-BCI technique.