PUBLISHED VERSION

Antenatal testosterone exposure influences fetal neurodevelopment and gender-role behavior in postnatal life and may contribute to differences in developmental psychopathology during childhood. We prospectively measured the associations between umbilical cord blood testosterone levels at birth and childhood behavioral development in both males and females from a large population based sample. The study comprised 430 females and 429 males from the Western Australian Pregnancy Cohort (Raine) Study where umbilical cord blood had been collected. Total testosterone concentrations were determined by mass spectrometry and bioavailable testosterone (BioT) levels were calculated. At two, five, eight and ten years of age, the participants completed the Child Behavior Checklist (CBCL). Linear regression models were used to analyse the relationship between BioT concentrations (in quartiles) and CBCL scores (total, internalizing, externalizing and selected syndrome). Boys had higher mean CBCL T-scores than girls across all ages of followup. There was no significant relationship between cord blood BioT quartiles and CBCL total, internalizing and externalizing T-scores at age two or five to ten combined. In the syndrome score analyses, higher BioT quartiles were associated with significantly lower scores for attention problems for boys at age five, eight and ten, and greater withdrawal symptoms in pre-school girls (age five). We did not identify a consistent relationship between antenatal testosterone exposure and total, internalizing or externalizing behavioral difficulties in childhood. Higher umbilical cord BioT levels were associated with lower scores for attention problems in boys up to 10 years and more withdrawn behavior in 5-year-old girls; however, these findings were not consistent across ages and require further investigation in a larger sample. Citation: Robinson M, Whitehouse AJO, Jacoby P, Mattes E, Sawyer MG, et al. (2013) Umbilical Cord Blood Testosterone and Childhood Internalizing and Externalizing Behavior: A Prospective Study. PLoS ONE 8(4): e59991. doi:10.1371/journal.pone. 0059991 Editor: C. Mary Schooling, CUNY, United States of America Received June 22, 2012; Accepted February 22, 2013; Published April 1, 2013 Copyright: 2013 Robinson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The authors acknowledge the support via core funding from the Raine Medical Research Foundation; The University of Western Australia (UWA); the Telethon Institute for Child Health Research; the UWA Faculty of Medicine, Dentistry and Health Science; the Women and Infants Research Foundation; and Curtin University. The Raine Study is also funded by the National Health and Medical Research Council of Australia (NHMRC, grant numbers 963209, 211912, 003209, and 353514), Australian Health Management, the Telstra Foundation, the Western Australian Health Promotion Foundation, the National Heart Foundation of Australia, and Beyond Blue. This research was funded by Australian Rotary Health. AW is supported by a NHMRC Career Development Fellowship (number 1004065). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: moniquer@ichr.uwa. edu.au