Miracle-dz/UniFAR-FTUs huggingface dataset:
Preparation of Sourdoughs Fermented with Isolated Lactic Acid Bacteria and Characterization of Their Antifungal Properties

Traditional sourdough is obtained using a mixture of flour and water stored at room temperature until acidification. Therefore, adding lactic acid bacteria (LAB) can improve the quality and safety of sourdough bread. Faced with this problem, four drying techniques—freeze-drying, spray-drying, low-temperature drying, and drying at low humidity—have been applied. Our goals were to isolate LAB strains with antifungal potential against Aspergillus and Penicillium fungi. The antifungal capacity was evaluated with agar diffusion, co-culture in overlay agar, and a microdilution susceptibility assay. In addition, the antifungal compounds generated in sourdough were analyzed. As a result, dried sourdoughs were prepared with Lactiplantibacillus plantarum TN10, Lactiplantibacillus plantarum TF2, Pediococcus pentosaceus TF8, Pediococcus acidilactici TE4, and Pediococcus pentosaceus TI6. The minimum fungicidal concentrations ranged from 25 g/L versus P. verrucosum and 100 g/L against A. flavus. A total of 27 volatile organic compounds were produced. Moreover, the lactic acid content reached 26 g/kg of dry product, and the phenyllactic concentration was significantly higher than the control. The P. pentosaceus TI6 exhibited a higher antifungal capacity in vitro and demonstrated a higher production of antifungal compounds compared to the other strains; therefore, further studies will evaluate the impact of this sourdough in bread manufacture.
Mass transport in layered fluid systems

The method of matched asymptotic expansions is employed to calculate the mass transport velocity due to small amplitude oscillatory waves propagating in conditions of density and viscosity discontinuities. For progressive waves in a two-layer system, it is found that the velocity at the interface is in the direction of wave propagation; when the uppermost surface is free, the velocity there is in the direction opposite to that at the interface. If the difference in the densities is small, the calculated transport velocity associated with an internal wave can be of more importance than that associated with the surface wave as obtained from the work of Longuet-Higgins (1953).
MUC1 Expressions and Its Prognostic Values in US Gastric Cancer Patients

Simple Summary Although the alteration of mucin (MUC) expression was associated with gastric cancer (GC) prognosis, its prognostic values remain to be elucidated in US GC patients. The positive expression of MUC1 was associated with aggressive pathologic features of GC including diffuse-type cancer, advanced cancer, lymph node metastasis, and distant metastasis. In early GC, patients with a high level of MUC1 expression showed a higher rate of lymphovascular invasion, which is a strong risk factor for lymph node metastasis and noncurative resection after endoscopic submucosal dissection. MUC1 expression can be a useful prognostic marker in US GC patients. Abstract This study aims to evaluate the prognostic value of MUC expression in US GC patients. A total of 70 tumor specimens were collected from GC patients who underwent surgery or endoscopic resection between 2013 and 2019 at a tertiary referral center in the US. MUC expression status including MUC1, MUC2, MUC5AC, and MUC6 was evaluated by immunohistochemical staining. The positive rates of MUC1, MUC2, MUC5AC, and MUC6 were 71.4%, 78.6%, 74.3%, and 33.3%, respectively. Patients with positive MUC1 expression had a significantly higher rate of aggressive pathologic features including diffuse-type cancer (42.0% vs. 0%; p < 0.001), advanced GC (80.0% vs. 30.0%, p < 0.001), lymph node metastasis (62.0% vs. 20.0%; p = 0.001), and distant metastasis (32.0% vs. 5.0%; p = 0.017) compared with those with negative MUC1 expression. However, the differences in the pathologic features were not observed according to MUC2, MUC5AC, and MUC6 expression status. In early gastric cancer (EGC), patients with a high level of MUC1 expression showed a higher rate of lymphovascular invasion (71.4% vs. 21.4%; p = 0.026) and EGC meeting non-curative resection (85.7% vs. 42.9%; p = 0.061) than those with negative MUC1. In US GC patients, MUC1 expression is associated with aggressive pathological features, and might be a useful prognostic marker.
Planned Extracorporeal Life Support Employment during Liver Transplantation: The Potential of ECMO and CRRT as Preventive Therapies—Case Reports and Literature Review

Liver Transplantation (LT) has become the gold standard treatment for End-Stage Liver Disease (ESLD). One of the main strategies to manage life-threatening complications, such as cardio-respiratory failure, is Extracorporeal Membrane Oxygenation (ECMO) in the peri-transplantation period, with different configurations of the technique and in combination with other extracorporeal care devices such as Continuous Renal Replacement Therapy (CRRT). This retrospective study includes three clinical cases of planned ECMO support strategies in LT and evaluates their application compared with current literature exploring PubMed/Medline. The three LT supported with ECMO and CRRT were performed at IRCCS Polyclinic S. Orsola-Malpighi, Bologna. All three cases of patients with compromised organ function analysed produced positive outcomes. The planned use of ECMO and CRRT support in peri-transplantation has allowed the patients to overcome contraindications and successfully undergo LT. In recent years, only a few reports have documented successful LT outcomes performed with intraoperative ECMO in critically ESLD patients. However, the management of LT with ECMO and/or CRRT assistance is an emerging challenge, with the need for more published evidence on this topic to guide treatment choices in patients with severe, acute and reversible respiratory and cardiovascular failure after LT.
Questioning European Democracy? Versions of Representation in the 15M Movement and Podemos

ABSTRACT In the wake of the social movement mobilization after the 2008 financial crisis in Spain, many would like to argue that this has given rise to a new wave of left-wing political representation, such as the party Podemos. Left-wing populism is often seen as the natural continuation of protest movements and hailed as reinvigorating democracy by creating new forms of representation for the previously unrepresented. This article argues that the political subjectivity espoused by Podemos hails from a long European tradition built on rationality, masculinity, and nationalism. In opposition, the political subjectivities practiced by the many branches of the 15M provide a more novel critique of the European democratic system. The article questions the claims to democratic innovation by Podemos, and argues that without a closer engagement with how current party practices reinforce different forms of exclusion, the democratic promise of left-wing populism may be weaker than previously thought.
Exploiting Visual Context Semantics for Sound Source Localization

Self-supervised sound source localization in unconstrained visual scenes is an important task of audio-visual learning. In this paper, we propose a visual reasoning module to explicitly exploit the rich visual context semantics, which alleviates the issue of insufficient utilization of visual information in previous works. The learning objectives are carefully designed to provide stronger supervision signals for the extracted visual semantics while enhancing the audio-visual interactions, which lead to more robust feature representations. Extensive experimental results demonstrate that our approach significantly boosts the localization performances on various datasets, even without initializations pretrained on ImageNet. Moreover, with the visual context exploitation, our framework can accomplish both the audio-visual and purely visual inference, which expands the application scope of the sound source localization task and further raises the competitiveness of our approach.
Flow Induced Pulsations in Gas Transport Systems: Analysis of the Influence of Closed Side Branches

A theoretical analysis is presented of the low frequency aero-acoustic behavior of closed side branches along a gas transport pipe. The theory predicts the hydrodynamic conditions for moderate and strong pulsations. A model is proposed which predicts the order of magnitude of the power generated by the aero-acoustic source. The theoretical analysis leads to the design of spoilers which reduce the pulsation level by 30 to 40 dB. The results obtained by theoretical analysis and model experiments (Reynolds number 10-6) have been confirmed in full scale tests (Reynolds number 10-8).
Chimeric Newcastle Disease Virus Vectors Expressing Human IFN-γ Mediate Target Immune Responses and Enable Multifaceted Treatments

The therapeutic potential of Newcastle disease virus (NDV) has been reported as both an oncolytic agent and a vaccine vector against many antigens. However, in the individuals already immunized with NDVs, second and subsequent administration does not provide substantial benefits. In this study, two types of recombinant chimeric NDVs using APMV-2 F and HN genes were generated. In rNDV-2HN, the wild-type NDV HN gene was replaced with the APMV-2 HN gene, and in rNDV-2F/2HN, both wild-type F and HN genes were replaced with APMV-2 F and HN genes, respectively. We enhanced the immune responses of these chimeric viruses by inserting the human IFN-γ gene. To examine the escape from NDV antiserum, each virus was treated with diluted NDV antiserum, and HEp-2 cells were infected with these virus particles. The two constructed chimeric viruses indicated notably lower virus-neutralizing titer compared to wild-type NDV and escaped the action of NDV antiserum. These two chimeric viruses infected both respiratory and colon cancer cell lines, indicating their potential as a cancer treatment tool. Chimeric viruses with enhanced immune responses can be considered a novel therapeutic strategy in cancer treatment that can be administered multiple times and used to enhance immune cells interaction.
iTB-test: An Intelligent Image-enabled Diagnostic System for In Vitro Screening of Infectious Diseases

This paper performs an investigation into the development of an intelligent image-based automatic in vitro diagnostic system for infectious diseases using personal devices. The proposed framework of the image-based diagnostic system is demonstrated using the case study of Tuberculosis (TB)-specific antibody detection. The developed system, denoted as the iTB-test, is an intelligent bio-sensing system, comprised of a plasmonic Enzyme-Linked Immunosorbent Assay based colourimetric test in combination with an artificial intelligence-enabled image-based system. The presented system can separate the region of interest with 99.62% accuracy using clustering-based hybrid image processing algorithms, whereas the classification accuracy of antibody detection using a supervised machine learning technique is 100% based on the experiments conducted for the case study.
Effects of Positive Fighting Experience and Its Subsequent Deprivation on the Expression Profile of Mouse Hippocampal Genes Associated with Neurogenesis

The hippocampus is known as the brain region implicated in visuospatial processes and processes associated with learning and short- and long-term memory. An important functional characteristic of the hippocampus is lifelong neurogenesis. A decrease or increase in adult hippocampal neurogenesis is associated with a wide range of neurological diseases. We have previously shown that in adult male mice with a chronic positive fighting experience in daily agonistic interactions, there is an increase in the proliferation of progenitor neurons and the production of young neurons in the dentate gyrus (in hippocampus), and these neurogenesis parameters remain modified during 2 weeks of deprivation of further fights. The aim of the present work was to identify hippocampal genes associated with neurogenesis and involved in the formation of behavioral features in mice with the chronic experience of wins in aggressive confrontations, as well as during the subsequent 2-week deprivation of agonistic interactions. Hippocampal gene expression profiles were compared among three groups of adult male mice: chronically winning for 20 days in the agonistic interactions, chronically victorious for 20 days followed by the 2-week deprivation of fights, and intact (control) mice. Neurogenesis-associated genes were identified whose transcription levels changed during the social confrontations and in the subsequent period of deprivation of fights. In the experimental males, some of these genes are associated with behavioral traits, including abnormal aggression-related behavior, an abnormal anxiety-related response, and others. Two genes encoding transcription factors (Nr1d1 and Fmr1) were likely to contribute the most to the between-group differences. It can be concluded that the chronic experience of wins in agonistic interactions alters hippocampal levels of transcription of multiple genes in adult male mice. The transcriptome changes get reversed only partially after the 2-week period of deprivation of fights. The identified differentially expressed genes associated with neurogenesis and involved in the control of a behavior/neurological phenotype can be used in further studies to identify targets for therapeutic correction of the neurological disturbances that develop in winners under the conditions of chronic social confrontations.
Highly Concurrent TCP Session Connection Management System on FPGA Chip

Transmission Control Protocol (TCP) is a connection-oriented data transmission protocol, and it is also the main communication protocol used for end-to-end data transmission in the current Internet. At present, the mainstream TCP protocol processing service is implemented by software running on the Central Processing Unit (CPU). However, with the rapid growth of transmission bandwidth and the number of connections, the software-based processing method is not ideal in terms of delay and throughput, and also affects the processing performance of the CPU in other applications such as virtualization services. Moreover, other hardware solutions can only support a limited number of TCP session connections. In order to improve the processing efficiency of the TCP protocol and achieve highly concurrent network services, this paper proposes a TCP offload engine (TOE) prototype system based on field programmable gate array (FPGA) chips. It not only provides hardware-based data path processing, but also realizes hardware management of large-scale TCP session connection status through a multi-level cache management mechanism. Studies have shown that this solution can support 100 Gbps high-performance throughput characteristics, and allow concurrent processing of hundreds to 250,000 TCP connection state hardware maintenance on a single network node, improving the overall performance of the network system.
Comparison of models for the physiological estimation of internal mechanical power in Cycling

Physiological models for estimating internal mechanical power (IP) generally share a common basis: the sum of IP and the external mechanical power (EP) is equal to the total mechanical power, as represented by net VO2. Â While a biomechanical perspective of IP argues against this simple summation, physiological models serve a valuable purpose because they account for the total flow of energy through the system. Â Despite their common foundation, estimates of IP using various physiological models have not been consistent. Â Two pre-existing models that use a physiological approach, as well as variations of them and a simple body mass-cadence relationship, were applied to submaximal data from eight well-trained male cyclists. Â Three incremental cycling tests were performed at cadences of 50-55, 80-85 and 110-115 rev â€¢ min-1. Â Differences in the mean and limits of agreement were used to show that values of IP calculated using two previously described models were not similar at any of the cadences tested. Â It was also shown that using relevant energy equivalents for converting VO2 into metabolic power produced smaller values for IP than when using a generic energy equivalent. Â Differences in values for IP in the published literature, therefore, might not necessarily be caused by differences in participant characteristics, but rather differences in the accuracy of the variables that are input into the IP models.
Influence on the Behavior of a Linear Dynamical System of Some Imposed Rapid Motions of Small Amplitude

Occasionally, linear dynamical system must operate under the influence of imposed motions of very small amplitude and high frequency which were not contemplated in its design. The influence of such motions on the behavior of the system is therefore of paramount interest. It is shown that, although the differential equations of motion are linear with oscillatory coefficients when the imposed motions are present, it is possible under certain conditions to determine the behavior in an average sense from the solution of a system of linear differential equations with constant coefficients. Criteria to determine the influence of the imposed motions on the stability and response to various inputs of the original system are easily found.
Clinical Application of Metformin Use in Anhui Province, China: A Cross-Sectional Study

Purpose Given the importance of metformin, reasonable utilization is essential. We designed a cross-sectional survey on physicians’ attitude and clinical application of metformin in Anhui Province, China. Methods The survey was distributed via an electronic questionnaire among endocrinologists and general practitioners. Seven representative questions were used to evaluate professional levels. Results Among the 477 valid responses, 72.75% of the respondents preferred to prescribe metformin extended-release, while only 34.38% of them would prescribe metformin extended-release at the correct frequency. More than half of the respondents thought that estimated glomerular filtration rate ˂ 45 mL/min/1.73 m² should be the contraindication of metformin prescription. Less than 10% of the physicians selected correct responses for two questions regarding metformin usage and contrast agent. Physicians with higher levels of hospital grades, education background and professional titles as well as working in general hospitals and in the Department of Endocrinology achieved high scores (P˂0.05). Logistic regression showed that department was an independent predictor for high scores. Conclusion Physicians, especially non-endocrinologists, are not at a professional level for prescribing metformin. Physicians should be highly vigilant in terms of standardized prescription for metformin. The guidelines or consensuses about diabetes care for physicians should be promoted.
Lysiphyllum strychnifolium (Craib) A. Schmitz Extracts Moderate the Expression of Drug-Metabolizing Enzymes: In Vivo Study to Clinical Propose

Lysiphyllum strychnifolium (Craib) A. Schmitz (LS) has been traditionally used as a medicinal herb by folk healers in Thailand with rare evidence-based support. Hepatic cytochrome P450s (CYPs450) are well known as the drug-metabolizing enzymes that catalyze all drugs and toxicants. In this study, we investigated the mRNA levels of six clinically important CYPs450, i.e., CYP1A2, 3A2, 2C11, 2D1, 2D2, and 2E1, in rats given LS extracts. Seventy Wistar rats were randomized into seven groups (n = 10). Each group was given LS stem ethanol (SE) and leaf water (LW) extracts orally at doses of 300, 2000, and 5000 mg/kg body weight (mg/kg.bw) for twenty-eight consecutive days. After treatment, the expression of CYPs450 genes was measured using quantitative real-time PCR. The results revealed that SE and LW, which contained quercetin and gallic acid, promoted the upregulation of all CYPs450. Almost all CYPs450 genes were downregulated in all male LW-treated rats but upregulated in female-treated groups, suggesting that CYP gene expressions in LS-treated rats were influenced by gender. Moderate and high doses of the LS extracts had a tendency to induce six CYP450s’ transcription levels in both rat genders. CYP2E1 gene showed a unique expression level in male rats receiving SE at a dose of 2000 mg/kg.bw, whereas a low dose of 300 mg/kg.bw was found in the LW-treated female group. As a result, our findings suggest that different doses of LS extracts can moderate the varying mRNA expression of clinically relevant CYP genes. In this study, we provide information about CYP induction and inhibition in vivo, which could be a desirable condition for furthering the practical use of LS extracts in humans.
Tweaking the Small Non-Coding RNAs to Improve Desirable Traits in Plant

Plant transcriptome contains an enormous amount of non-coding RNAs (ncRNAs) that do not code for proteins but take part in regulating gene expression. Since their discovery in the early 1990s, much research has been conducted to elucidate their function in the gene regulatory network and their involvement in plants’ response to biotic/abiotic stresses. Typically, 20–30 nucleotide-long small ncRNAs are a potential target for plant molecular breeders because of their agricultural importance. This review summarizes the current understanding of three major classes of small ncRNAs: short-interfering RNAs (siRNAs), microRNA (miRNA), and transacting siRNAs (tasiRNAs). Furthermore, their biogenesis, mode of action, and how they have been utilized to improve crop productivity and disease resistance are discussed here.
Jointly Learning Band Selection and Filter Array Design for Hyperspectral Imaging

A single-shot multispectral camera equipped with an optimized color filter array (CFA) has the potential to deliver a fast and low-cost hyperspectral (HS) imaging system. Previous solutions are largely restricted to designing demosaicing algorithms for fixed CFAs – be it the Bayer color pattern or evenly-spaced spectral multiplexing patterns. Since sampling and reconstruction are tightly-coupled, the ability to search for an optimal solution is severely constrained by using predefined CFAs. In this work, we simultaneously address the problem of spectral band selection, CFA design, image demosaicing, and spectral image recovery in a joint learning framework for single-shot HS imaging. We propose a reinforcement learning (RL) based method for spectral band selection and a novel neural network for CFA generation, image demosaicing, and HS image recovery. The final spectral reconstruction accuracy is used to supervise the training of the main network to maximize the synergies between those tightly-related tasks. The RL method regards the main network as an agent to collect reward. Our final method delivers a simple setup – as simple as an RGB camera – for HS imaging. Experimental results show that our method outperforms competing methods by a large margin.
Enhanced gabor-based region covariance matrices for palmprint recognition

An enhanced Gabor-based region covariance matrices (EGRCM) method for palmprint recognition is proposed. Motivated by the fact that both the Gabor magnitude (GM) and the Gabor phase (GP) of a certain image contain effective information for image feature extraction, they are utilised simultaneously to construct the proposed EGRCM image descriptor for palmprint recognition. Experimental results demonstrate the efficacy of the proposed method.