Miracle-dz/UniFAR-FTUs huggingface dataset:
Deletion of the lipid droplet protein kinase gene affects lipid droplets biogenesis, parasite infectivity, and resistance to trivalent antimony in Leishmania infantum

The Lipid Droplet Protein Kinase (LDK) facilitates lipid droplet (LD) biogenesis, organelles involved in various metabolic and signaling functions in trypanosomatids. As LDK’s function has not been previously explored in Leishmania spp. , we utilized CRISPR/Cas9 technology to generate LDK-knockout lines of Leishmania infantum to investigate its role in this parasite. Our findings demonstrate that LDK is not an essential gene for L. infantum, as its deletion did not impede parasite survival. Furthermore, removing LDK did not impact the growth of promastigote forms of L. infantum lacking LDK. However, a noticeable reduction in LDs occurred during the stationary phase of parasite growth following LDK deletion. In the presence of myriocin, a LD inducer, LDK-knockout parasites displayed reduced LD abundance during both logarithmic and stationary growth phases compared to control parasites. Moreover, an infection analysis involving THP-1 cells revealed that 72 h post-infection, LDK-knockout L. infantum lines exhibited fewer infected macrophages and intracellular amastigotes than control parasites. LDK-knockout L. infantum lines also displayed 1.7 to 1.8 -fold greater resistance to trivalent antimony than control parasites. There were no observed alterations in susceptibility to amphotericin B, miltefosine, or menadione in LDK-knockout L. infantum lines. Our results suggest that LDK plays a crucial role in the biogenesis and/or maintenance of LDs in L. infantum, as well as in parasite infectivity and resistance to trivalent antimony.
The Multifaceted Role of Oxytocinergic System and OXTR Gene

The article explores the multifaceted role of the neuropeptide oxytocin in human behavior and its connection to the oxytocin receptor ( OXTR ) gene. Oxytocin, produced in specific brain nuclei, is implicated in emotional, social, and maternal behaviors, stress reduction, uterine contraction during childbirth, and lactation. The OXTR gene, located on chromosome 3, encodes oxytocin receptors found in various body parts, including critical brain regions associated with social behaviors. The article delves into studies on rodents, revealing correlations between OXTR gene expression and pair bonding in the prefrontal cortex and social behavior regulation in the amygdala. The discussion extends to the impact of oxytocin on social support-seeking behavior, focusing on a specific genetic variation, rs53576. The article explores how this genetic variation influences empathy, stress reactivity, and susceptibility to disorders such as autism and social anxiety. Furthermore, the article examines structural and functional changes in the brain associated with OXTR gene variations. It discusses the role of DNA methylation in influencing oxytocin receptor availability, affecting social perception and responsiveness to negative stimuli. The article also highlights the oxytocinergic system's involvement in disorders such as autism and social anxiety, emphasizing the interplay between genetics and environmental factors. The article also touches on the potential therapeutic use of exogenous oxytocin in mitigating symptoms associated with these disorders. In summary, the article underscores the intricate relationship between oxytocin, the OXTR gene, and diverse aspects of human behavior, providing insights into social bonding, perception, and the development of behavioral disorders.
Pharmacological activity of capsaicin: Mechanisms and controversies (Review)

Capsaicin, which is abundant in chili peppers, exerts antioxidative, antitumor, antiulcer and analgesic effects and it has demonstrated potential as a treatment for cardiovascular, gastrointestinal, oncological and dermatological conditions. Unique among natural irritants, capsaicin initially excites neurons but then ‘calms’ them into long-lasting non-responsiveness. Capsaicin can also promote weight loss, making it potentially useful for treating obesity. Several mechanisms have been proposed to explain the therapeutic effects of capsaicin, including antioxidation, analgesia and promotion of apoptosis. Some of the mechanisms are proposed to be mediated by the capsaicin receptor (transient receptor potential cation channel subfamily V member 1), but some are proposed to be independent of that receptor. The clinical usefulness of capsaicin is limited by its short half-life. The present review provided an overview of what is known about the therapeutic effects of capsaicin and the mechanisms involved and certain studies arguing against its clinical use were mentioned.
A retrospective observation of virologically suppressed people living with HIV by comparing switching to BIC/TAF/FTC with initial use BIC/TAF/FTC

Abstract Background The objective of this study was to observe retrospectively the clinical response of virologically suppressed people living with HIV (PLWH) by comparing switching to BIC/TAF/FTC with initial use BIC/TAF/FTC. Methods PLWH using BIC/TAF/FTC was divided into ‘initial use’ group and ‘switching to’ group. Immune response, metabolic parameters and renal function between the two groups were analysed. Results The CD4 cell counts was higher in post- treatment than pre- treatment in the ‘switching to’ group (416.54 ± 212.11 cells/mm3 vs. 243.72 ± 156.64 cells/mm3, p < .001); however, significant differences were not observed in the ‘initial use’ group (p = .658). The effect of BIC/TAF/FTC on metabolism was not obvious. Serum creatinine (SCr) was improved in post-treatment than in pre-treatment in ‘switching to’ group (69.03 ± 18.78 vs. 77.52 ± 20.18, p < .001). Platelet count was lower in post-treatment than pre-treatment both in the ‘initial use’ group (175.81 ± 69.27 vs. 202.90 ± 66.56, p = .070) and in the ‘switching to’ group (177.04 ± 64.48 vs. 212.53 ± 63.43, p < .001). Conclusions ‘Switching to’ is superior to ‘initial use’ BIC/TAF/FTC in immune response among PLWH. The effect of BIC/TAF/FTC on metabolism is not obvious. BIC/TAF/FTC related thrombocytopenia needs to be further explored.
Cascaded Plane Wave Ultrasound for Blood Velocity Vector Imaging in the Carotid Artery

Cascaded dual-polarity waves (CDWs) imaging increases the signal-to-noise ratio (SNR) by transmitting trains of pulses with different polarity order, which are combined via decoding afterward. This potentially enables velocity vector imaging (VVI) in more challenging SNR conditions. However, the motion of blood in between the trains will influence the decoding process. In this work, the use of CDW for blood VVI is evaluated for the first time. Dual-angle, plane wave (PW) ultrasound, CDW-coded, and noncoded conventional PW (cPW), was acquired using a 7.8 MHz linear array at a pulse repetition frequency (PRF) of 8 kHz. CDW-channel data were decoded prior to beamforming and cross correlation-based compound speckle tracking for VVI. Simulations of single scatterer motion show a high dependence of amplitude gain on the velocity magnitude and direction for CDW-coded transmissions. Both simulations and experiments of parabolic flow show increased SNRs for CDW imaging. As a result, CDW outperforms cPW VVI in low SNR conditions, based on both bias and standard deviation (SD). Quantitative linear regression and qualitative analyses of simulated realistic carotid artery blood flow show a similar performance of CDW and cPW for high SNR (14 dB) conditions. However, reducing the SNR to 6 dB, results in a root-mean-squared error $2.7\times $ larger for cPW versus CDW, and an ${R}^{{2}}$ of 0.4 versus 0.9. Initial in vivo evaluation of a healthy carotid artery shows increased SNR and more reliable velocity estimates for CDW versus cPW. In conclusion, this work demonstrates that CDW imaging facilitates improved VVI of deeper located carotid arteries.
2006 AIB Southeast Asia Regional Conference Global Vision and Local Competence

Recently, production of Tamiflu is a controversial issue. Because of Tamiflu is a patent right owning to Roche. The protection of private property right is a most important principle for capitalism. Why some country want to produce Tamiflu before influenza be pandemic in spite of protection of patent? And latter why Roche granted approval of an additional manufacturing site in another country for increasing production capacity? Because of life-saving is first allocation value of legitimacy over profit of patent protection at present. State has the supreme power to define what is legitimate, when social, economic and political environment change, state will change these value ordering. If MNEs can fit more the values allocation of state, they gain more legitimacy to survive. State is a mediator for MNES to detect changing legitimacy. MNEs could not just conform to other organization, but they must conform to the changing legitimacy to adjust themselves for gaining more resource. This study tries to discuss the reason behind the isomorphism, not just for conformation. If the reason of legitimacy changes, then organization would be change their isomorphic action for adequate response. Keyword: legitimacy, institution theory, patent protection, political system
How do passive infrared triggered camera traps operate and why does it matter? Breaking down common misconceptions

The use of passive infrared (PIR) triggered camera traps has dramatically increased in recent decades. Unfortunately, technical descriptions of how PIR triggered camera traps operate have not been sufficiently clear. Descriptions have often been ambiguous or misleading and in several cases are demonstrably wrong. Such descriptions have led to erroneous interpretations of camera trapping data. This short communication clarifies how PIR sensors operate. We clarify how infrared radiation is emitted and transmitted, and we describe the parts of the PIR sensor and how they detect infrared radiation and, by extension, fauna. Several problematic descriptions of PIR sensors are drawn on to highlight flawed descriptions and demonstrate where erroneous interpretations of camera trapping data occurred. By clarifying the language and the description of PIR triggered camera traps, this paper ensures that wildlife researchers and managers using camera traps will avoid flawed interpretations of their data. Avoiding flawed interpretations of data should reduce wasted effort and resources that would otherwise come about as researchers attempt to test flawed hypotheses. Furthermore, this paper provides a thorough technical reference for camera trapping practitioners, which is not present elsewhere in the wildlife research literature.
European Forest Recreation and Tourism : A Handbook

Introduction 1. Forest Recreation and Nature Tourism in Europe: Context, history, and current Situation Part 1 2. Evaluating the Economic and Social Benefits of Forest Recreation and Nature Tourism 3. Integrating Forest Recreation and Nature Tourism into the Rural Economy 4. Instruments for Developing Recreation and Nature Tourism in Forests Part 2 5. Monitoring of Forest Recreation Demand 6. Assessing and Planning the Supply of Opportunities for Forest Recreation and Nature Tourism Part 3 7. Strategic Planning of Forest Recreation and Nature Tourism 8. The Recreation Planning Process 9. Site Planning and Design for Recreation and Nature Tourism
CT image denoising methods for image quality improvement and radiation dose reduction

Abstract With the ever‐increasing use of computed tomography (CT), concerns about its radiation dose have become a significant public issue. To address the need for radiation dose reduction, CT denoising methods have been widely investigated and applied in low‐dose CT images. Numerous noise reduction algorithms have emerged, such as iterative reconstruction and most recently, deep learning (DL)‐based approaches. Given the rapid advancements in Artificial Intelligence techniques, we recognize the need for a comprehensive review that emphasizes the most recently developed methods. Hence, we have performed a thorough analysis of existing literature to provide such a review. Beyond directly comparing the performance, we focus on pivotal aspects, including model training, validation, testing, generalizability, vulnerability, and evaluation methods. This review is expected to raise awareness of the various facets involved in CT image denoising and the specific challenges in developing DL‐based models.
method for measuring the three-dimensional orientation of cortical anals with implications for comparative analysis of bone icrostructure in vertebrates saac

The orientation of vascular canals in cortical bone can reveal information about the growth rate and loading environment of a bone. For example, in birds it has been proposed that a high proportion of circumferential canals (a laminar cortex) is related to fast growth or torsional loading related to active flight. In this paper we present a method to measure the three dimensional (3D) orientation of vascular canals. Image data are obtained from micro-CT and two angles are measured: phi, determining how longitudinal a canal is; and theta, determining whether a canal is radial or circumferential. This method can measure the orientation of each canal contained in the scanned images. Here we demonstrate the approach on two samples − a rat tibia and a hawk humerus. This method offers a direct (3D) method for quantifying features of canal orientation, such as the degree of laminarity, and can be applied easily
An acoustic investigation of the near-surface turbulence on Mars.

The Perseverance rover is carrying out an original acoustic experiment on Mars: the SuperCam microphone records the spherical acoustic waves generated by laser sparks at distances from 2 m to more than 8 m. These N-shaped acoustic waves scatter from the multiple local heterogeneities of the turbulent atmosphere. Therefore, large and random fluctuations of sound travel time and intensity develop as the waves cross the medium. The variances of the travel times and the scintillation index (normalized variance of the sound intensity) are studied within the mathematical formalism of the propagation of spherical acoustic waves through thermal turbulence to infer statistical properties of the Mars atmospheric temperature fluctuation field. The comparison with the theory is made by simplifying assumptions that do not include wind fluctuations and diffraction effects. Two Earth years (about one Martian year) of observations acquired during the maximum convective period (10:00-14:00 Mars local time) show a good agreement between the dataset and the formalism: the travel time variance diverges from the linear Chernov solution exactly where the density of occurrence of the first caustic reaches its maximum. Moreover, on average, waves travel faster than the mean speed of sound due to a fast path effect, which is also observed on Earth. To account for the distribution of turbulent eddies, several power spectra are tested and the best match to observation is obtained with a generalized von Karman spectrum with a shallower slope than the Kolmogorov cascade, ϕ(k)∝(1+k2L2)-4/3. It is associated with an outer scale of turbulence, L, of 11 cm at 2 m above the surface and a standard deviation of 6 K over 9 s for the temperature. These near-surface atmospheric properties are consistent with a weak to moderate wave scattering regime around noon with little saturation. Overall, this study presents an innovative and promising methodology to probe the near-surface atmospheric turbulence on Mars.
Assessing ecological communities in the wake of ongoing land use change in the Atlantic Forest and Pantanal of Brazil

Globally, land use change (LUC) is responsible for 20% of anthropogenic CO2 emissions, widespread losses of ecosystem services and biodiversity, and erosion of ecosystem resilience to disturbance and climate change. Although LUC has peaked in developed northern temperate countries, it is ongoing in wilderness-rich equatorial countries, where economic and governmental incentives continue to drive expansion of agricultural development and natural resource extraction. In Brazil, vast tracts of tropical forest and other types of natural vegetation cover have been (and are being) replaced by crops and planted exotic (non-native) pasture, the latter for livestock rearing. I investigated how a range of ecological communities are being affected by ongoing LUC in two Neotropical biomes, the Atlantic Forest and Pantanal of Brazil. My objectives were to: (1) help understand the consequences of LUC, (2) provide baselines for current and future monitoring studies, and (3) help prioritize conservation actions. The Atlantic Forest, which originally covered 1.5 million km 2 of eastern Brazil, is a biodiversity hotspot with a range of tropical forest formations and a high proportion of endemic species. Five-hundred years of LUC, including coffee and sugarcane cultivation, timber extraction, wood charcoal production for industry, and urban development, shrank forested area to 11% of original coverage, creating a highly-fragmented landscape of mostly small (<100 ha) forest patches within an agriculturally-dominated matrix. In a fragmented region of inland seasonal forest, called the Planalto or interior Atlantic Forest, I investigated the state of headwater stream fish communities 80 years after much of the original vegetation was cleared for coffee plantations. The Pantanal, which covers 150,500 km 2 in central-western Brazil, southeastern Bolivia and northeastern Paraguay, is a highly-seasonal floodplain comprised of tropical forest, savanna, and wetland formations supporting large populations of fishes, waterbirds, and charismatic wildlife species. Since the late 1800s, LUC in the Pantanal has centered around extensive cattle ranching and more recently (since the 1960s) the expansion of grazing lands through conversions of native vegetation to planted exotic pasture. In the southern Pantanal, I investigated how cattle activity and ranching practices are affecting: (1) the
Soil greenhouse gas emissions from inorganic fertilizers and recycled oil palm waste products from Indonesian oil palm plantations

A continuous rise in the global demand for palm oil has resulted in the large‐scale expansion of oil palm plantations and generated environmental controversy. Efforts to increase the sustainability of oil palm cultivation include the recycling of oil mill and pruning residues in the field, but this may increase soil methane (CH4) emissions. This study reports the results of yearlong field‐based measurements of soil nitrous oxide (N2O) and CH4 emissions from commercial plantations in North Sumatra, Indonesia. One experiment investigated the effects of soil‐water saturation on N2O and CH4 emissions from inorganic fertilizers and organic amendments by simulating 25 mm rainfall per day for 21 days. Three additional experiments focused on emissions from (a) inorganic fertilizer (urea), (b) combination of enriched mulch with urea and (c) organic amendments (empty fruit bunches, enriched mulch and pruned oil palm fronds) applied in different doses and spatial layouts (placed in inter‐row zones, piles, patches or bands) for a full year. The higher dose of urea led to a significantly higher N2O emissions with the emission factors ranging from 2.4% to 2.7% in the long‐term experiment, which is considerably higher than the IPCC standard of 1%. Organic amendments were a significant source of both N2O and CH4 emissions, but N2O emissions from organic amendments were 66%–86% lower than those from inorganic fertilizers. Organic amendments applied in piles emitted 63% and 71% more N2O and CH4, respectively, than when spread out. With twice the dose of organic amendments, cumulative emissions were up to three times greater. The (simulated) rainwater experiment showed that the increase in precipitation led to a significant increase in N2O emissions significantly, suggesting that the time of fertilization is a critical management option for reducing emissions. The results from this study could therefore help guide residue and nutrient management practices to reduce emissions while ensuring better nutrient recycling for sustainable oil palm production systems.
pH and ROS Responsiveness of Polymersome Nanovaccines for Antigen and Adjuvant Codelivery: An In Vitro and In Vivo Comparison

The antitumor immunity can be enhanced through the synchronized codelivery of antigens and immunostimulatory adjuvants to antigen-presenting cells, particularly dendritic cells (DCs), using nanovaccines (NVs). To study the influence of intracellular vaccine cargo release kinetics on the T cell activating capacities of DCs, we compared stimuli-responsive to nonresponsive polymersome NVs. To do so, we employed “AND gate” multiresponsive (MR) amphiphilic block copolymers that decompose only in response to the combination of chemical cues present in the environment of the intracellular compartments in antigen cross-presenting DCs: low pH and high reactive oxygen species (ROS) levels. After being unmasked by ROS, pH-responsive side chains are exposed and can undergo a charge shift within a relevant pH window of the intracellular compartments in antigen cross-presenting DCs. NVs containing the model antigen Ovalbumin (OVA) and the iNKT cell activating adjuvant α-Galactosylceramide (α-Galcer) were fabricated using microfluidics self-assembly. The MR NVs outperformed the nonresponsive NV in vitro, inducing enhanced classical- and cross-presentation of the OVA by DCs, effectively activating CD8+, CD4+ T cells, and iNKT cells. Interestingly, in vivo, the nonresponsive NVs outperformed the responsive vaccines. These differences in polymersome vaccine performance are likely linked to the kinetics of cargo release, highlighting the crucial chemical requirements for successful cancer nanovaccines.
Additional contribution of phonophoresis and low-level laser therapy to exercise in the treatment of carpal tunnel syndrome: A clinical, electrophysiological, and ultrasonographic evaluation.

OBJECTIVES Carpal Tunnel Syndrome (CTS) is the most common peripheral nerve compression syndrome. This study aims to evaluate the additive contribution of phonophoresis and low-level laser therapy (LLLT) to tendon and nerve gliding exercises electrophysiologically, ultrasonographically, and clinically in the treatment of moderate CTS. METHODS The sample consisted of 45 patients with moderate CTS, randomized into three groups. Group 1 received phonophoresis and exercise, Group 2 received LLLT and exercise, and Group 3 received exercise alone. Participants were evaluated electrophysiologically, clinically, and ultrasonographically before treatment and at the 6th and 12th weeks after the treatment. RESULTS An improvement was observed in clinical parameters (Boston Functional Status Scale, Boston Symptom Severity Scale, visual analog scale at rest, and visual analog scale during activity) for all groups at the 6th and 12th weeks after the treatment (p<0.05). An improvement was also noted in the ultrasonographic parameter (cross-sectional area) for all groups at the 12th week after the treatment (p=0.017). CONCLUSION Phonophoresis and LLLT do not provide additional effects to exercise therapy. Exercise therapy alone may positively contribute to ultrasonographic and clinical outcomes in the treatment of moderate CTS.