Miracle-dz/UniFAR-FTUs huggingface dataset:
Preliminary Study on Rapid and Simultaneous Detection of Viable Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella by PMA-mPCR in Food

Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella are major foodborne pathogens that are widespread in nature and responsible for several outbreaks of food safety accidents. Thus, a rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. To eliminate false positive results, propidium monoazide (PMA) was applied to selectively suppress the DNA amplification of dead cells. The results showed the optimum concentration of PMA is 5.0 µg/mL. The detection limit of this assay by mPCR was 103 CFU/mL in the culture broth, and by PMA-mPCR was 104 CFU/mL both in pure culture and a food matrix (milk and ground beef). In addition, the detection of mixed viable and dead cells was also explored in this study. The detection sensitivity ratio of viable and dead counts was less than 1:10. Therefore, the PMA-mPCR assay proposed here might provide an efficient detection tool for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella and also have great potential for the detection and concentration assessment of VBNC cells.
Olanzapine Overdose in a Two-Year-Old Girl Resulting in Both High Serum and Plasma Levels

The antipsychotic olanzapine is used increasingly to treat various psychiatric illnesses. Accidental olanzapine overdose is uncommon among children. Here, we report a case of a child presenting with an unexplained coma. Accidental ingestion of olanzapine (20 mg) was confirmed by measurement of drug concentrations in both serum and plasma.
Mittag‐Leffler state estimator design and synchronization analysis for fractional‐order BAM neural networks with time delays

This paper deals with the extended design of Mittag‐Leffler state estimator and adaptive synchronization for fractional‐order bidirectional associative memory neural networks with time delays. By the aid of Lyapunov direct approach and Razumikhin‐type method, a suitable fractional‐order Lyapunov functional is constructed and a new set of novel sufficient condition are derived to estimate the neuron states via available output measurements such that the ensuring estimator error system is globally Mittag‐Leffler stable. Then, the adaptive feedback control rule is designed, under which the considered FBNNs can achieve Mittag‐Leffler adaptive synchronization by means of some fractional‐order inequality techniques. Moreover, the adaptive feedback control may be utilized even when there is no ideal information from the system parameters. Finally, two numerical simulations are given to reveal the effectiveness of the theoretical consequences.
Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model

The exact mechanism of desmoplastic stromal reaction (DSR) formation is still unclear. The interaction between cancer cells and cancer-associated fibroblasts (CAFs) has an important role in tumor progression, while stromal changes are a poor prognostic factor in pleural mesothelioma (PM). We aimed to assess the impact of CAFs paracrine signaling within the tumor microenvironment and the DSR presence on survival, in a cohort of 77 PM patients. DSR formation was evaluated morphologically and by immunohistochemistry for Fibroblast activation protein alpha (FAP). Digital gene expression was analyzed using a custom-designed CodeSet (NanoString). Decision-tree-based analysis using the “conditional inference tree” (CIT) machine learning algorithm was performed on the obtained results. A significant association between FAP gene expression levels and the appearance of DSR was found (p = 0.025). DSR-high samples demonstrated a statistically significant prolonged median survival time. The elevated expression of MYT1, KDR, PIK3R1, PIK3R4, and SOS1 was associated with shortened OS, whereas the upregulation of VEGFC, FAP, and CDK4 was associated with prolonged OS. CIT revealed a three-tier system based on FAP, NF1, and RPTOR expressions. We could outline the prognostic value of CAFs-induced PI3K signaling pathway activation together with FAP-dependent CDK4 mediated cell cycle progression in PM, where prognostic and predictive biomarkers are urgently needed to introduce new therapeutic strategies.
Psychosocial Problems on Adolescents with Thalassemia Major: A Systematic Scoping Review

Disease and treatment of thalassemia major that are carried out for life can cause physical and psychological problems in sufferers. Adolescents with thalassemia major will experience disturbances at a stage of development that is undergoing dynamic changes. Nurses, as health workers who provide holistic services, need to know what psychosocial problems may arise in adolescents with thalassemia major. This study aims to describe psychosocial problems in adolescents with thalassemia major. This study uses a scoping review design by searching English literature through the CINAHL, PubMed, and Scopus databases. The keywords used are psychosocial problems, thalassemia major, and adolescence. The criteria for the article in this study where the patient was a teenager with thalassemia, open access full text, and the time setting was the last 10 years (2013-2022). We found 9 articles discussing psychosocial problems in adolescents with thalassemia. Psychosocial problems that arise in the form of disturbances in several functions, namely physical, emotional, social, and school functions. Psychosocial disorders that occur in adolescents with thalassemia cause disturbances at the stage of adolescent development. Psychosocial problems that occur in adolescents with thalassemia include impaired physical, emotional, social, and school functions. Keywords: adolescence, psychosocial problems, thalassemia major
Peculiarities of the Variation of Biologically Active Compounds in Fruit of Vaccinium oxycoccos L. Growing in the Čepkeliai State Strict Nature Reserve

This study was carried out to analyze the accumulation patterns of anthocyanins, proanthocyanidins, flavonols, chlorogenic acid, and triterpene compounds in fruit samples of Vaccinium oxycoccos L. berries growing in the Čepkeliai State Strict Nature Reserve in Lithuania. Studies were carried out on the phytochemical composition of cranberry fruit samples during the period of 2020–2022. Anthocyanins, flavonols, chlorogenic acid and triterpene compounds were identified and quantified using UPLC-DAD methods, and proanthocyanins were determined using spectrophotometric methods. The content of identified compounds varied, as reflected in the total amounts of anthocyanins (710.3 ± 40 µg/g to 6993.8 ± 119 µg/g), proanthocyanidins (378.4 ± 10 µg EE/g to 3557. 3 ± 75 µg EE/g), flavonols (479.6 ± 9 µg/g to 7291.2 ± 226 µg/g), chlorogenic acid (68.0 ± 1 µg/g to 3858.2 ± 119 µg/g), and triterpenoids (3780.8 ± 98 µg/g to 7226.9 ± 224 µg/g). Cranberry fruit samples harvested from open oligotrophic wetland habitats contained higher levels of anthocyanins, anthocyanidins, flavonol glycosides, and proanthocyanidins. The highest levels of triterpene compounds were found in the cranberry fruits harvested in the spring of the following year after the snowmelt. The use of principal component analysis showed that cranberry plant material harvested in October and November had higher levels of bioactive compounds.
Job Satisfaction in Banking Sector: An Exploratory Analysis

This paper attempts to focus on job satisfaction in banking sector. The total sample covers 362 bank employees working in both public and private sector banks. To study job satisfaction, dimensions like job aspects, work environment, innovative aspects and interpersonal relations are focused. Further, variables like age, sex, marital status, qualification and type of organization are considered for the analysis. A study into relationship between the dimensions reveals that a significant relationship between dimensions under study is existed. More over dimensional-wise analysis reveals that innovative and work environment as well as job aspects and interpersonal relations are strongly correlated. While job aspects and innovative aspects were associated relatively very low, differences in opinion between the variable-wise respondents is also revealed by the study.
A MULTIPLE REMOTE SENSOR SYSTEM FOR THE AERIAL SURVEILLANCE OF THE NORTH SEA AND BALTIC SEA

A MULTIPLE REMOTE SENSOR SYSTEM FOR THE AERIALSURVEILLANCE OF THE NORTH SEA AND BALTIC SEA*O. Trieschmann, Th. Hunsanger, U. B. BarjenbruchGerman Federal Institute of Hydrology56068 Koblenz, GermanyEmail: trieschmann@bafg.deABTRACTMarine pollution in the sensible North Sea and Baltic Sea forces aninternational aerial surveillance. Within this framework, the German aerialsurveillance system operates an advanced instrumentation on board of two ‘Dornier228’ aircrafts. The instrumentation consists of a set of remote sensors within animproved sensor network of the second generation. The aim is to detect oil spills onthe water surface, emitted accidentally or illegally. For surveillance purposes thespills have to be located within a large area. If spills were identified, the pollution hasto be classified and quantified with a high accuracy.This paper describes the set of instruments and their potential to fulfill thesedemands. A sideward looking airborne radar (SLAR) operates to locate the discharge,whereas a line scanning infrared/ultraviolet line scanner (IR/UV) allows to examinethe polluted area more precisely with a higher resolution. The IR/UV sensor andespecially the line scanning microwave radiometer (MWR) on board the aircraftallow to quantify the thickness of the oil film. A laser-fluoro line scanning sensor(LFS) classifies the oil as well as organic material, chlorophyll, and gelbstoff. Aforward looking infrared imaging camera completes the set of instruments that allowto convict the polluter. A detailed technical description of the sensors is presentedwith respect to the specific interests of potential administrative and scientific users.Results of sensor measurements and their synergy effects are given.1. 0 INTRODUCTIONDuring the last two decades, the German Federal Ministry of Transport set up an airbornesurveillance system to monitor the German territorial waters in the North Sea and Baltic Sea for oildischarges and marine pollution. These activities are within an international effort culminated in theMARPOL Agreement and HELSINKI Convention defining an international standard on the protectionand surveillance of the North Sea and Baltic Sea. The airborne maritime surveillance system consisted oftwo DO 28 aircraft each equipped with a Side-Looking Airborne Radar (SLAR) for detecting oil slicksover large distances, a UV/IR scanner for mapping the sea surface in the nadir range, and TV andphotographic cameras for the identification of the polluter and for collecting evidence.
Abscisic acid-induced response of Triticum aestivum and T. spelta phytohormonal system to moderate soil drought

The effect of abscisic acid (ABA) priming (10 −6 M) on the growth and hormonal homeostasis of winter wheat ( Triticum aestivum L.) and spelt wheat ( T. spelta L.) plants after drought stress was analysed. During the experiment, 18-day-old water-germinated (C-plants) and ABA-primed (ABA + -plants) plants exposed to drought stress (4 days without irrigation) and 21-day-old plants after recovery were studied. Drought-stressed ABA + -plants showed increased length, fresh (FW) and dry (DW) weight of roots and decreased shoot height and FW compared to C-plants. Priming with ABA increased the accumulation of ABA in T. aestivum and T. spelta plants during soil drought. However, in T. spelta ABA + -plants, the ABA content was 2.2-fold greater than in T. aestivum . After recovery, the ABA content in both species decreased. Drought stress decreased indole acetic acid (IAA) content in T. aestivum ABA + -plants but increased in T. spelta ones. The IAA content in stressed ABA + -plants of both species was 1.6-fold higher compared to stressed C-plants. After recovery, IAA accumulated in both ABA + -plants. In stressed T. aestivum and T. spelta ABA + -plants, gibberellic acid (GA 3 ) content was 123.4% and 24.2% higher compared to C-plants. In recovered ABA + -plants, GA 3 content was higher than in C-plants. The content of salicylic acid (SA) in stressed T. aestivum and T. spelta ABA + -plants increased by 5.5% and 62.8%, respectively. Total cytokinin (CK) content in stressed T. aestivum and T. spelta ABA + -plants did not recover the level of stressed С-plants and after recovery was lower by 43% and 56.8%, respectively. Changes in CK forms in all treatments of the experiment were observed. Overall, the priming of T. aestivum and T. spelta seeds with ABA improved root growth and was associated with changes in the hormonal system of soil drought-stressed plants. The results of the experiment suggest that long-term changes in the balance of endogenous phytohormones caused by the ABA priming are related with the formation of drought stress response.
Adversarial Obstacle Generation Against LiDAR-Based 3D Object Detection

LiDAR sensors are widely used in many safety-critical applications such as autonomous driving and drone control, and the collected data called point clouds are subsequently processed by 3D object detectors for visual perception. Recent works have shown that attackers can inject virtual points into LiDAR sensors by strategically transmitting laser pulses to them; additionally, deep visual models have been found to be vulnerable to carefully crafted adversarial examples. Therefore, a LiDAR-based perception may be maliciously attacked with serious safety consequences. In this article, we present a highly-deceptive adversarial obstacle generation algorithm against deep 3D detection models, to mimic fake obstacles within the effective detection range of LiDAR using a limited number of points. To achieve this goal, we first perform a physical LiDAR simulation to construct sparse obstacle point clouds. Then, we devise a strong attack strategy to adversarially perturb prototype points along each direction of the ray. Our method achieves a high attack success rate while complying with physical laws at the hardware level. We perform comprehensive experiments on different types of 3D detectors and determine that the voxel-based detectors are more vulnerable to adversarial attacks than the point-based methods. For example, our approach achieves an 89% mean attack success rate against PV-RCNN by using only 20 points to spoof a fake car.
Effect of fl ecainide derivatives on sarcoplasmic reticulum calcium release suggests a lack of direct action on the cardiac ryanodine receptor Correspondence

BACKGROUND AND PURPOSE Flecainide is a use-dependent blocker of cardiac Na channels. Mechanistic analysis of this block showed that the cationic form of flecainide enters the cytosolic vestibule of the open Na channel. Flecainide is also effective in the treatment of catecholaminergic polymorphic ventricular tachycardia but, in this condition, its mechanism of action is contentious. We investigated how flecainide derivatives influence Ca-release from the sarcoplasmic reticulum through the ryanodine receptor channel (RyR2) and whether this correlates with their effectiveness as blockers of Na and/or RyR2 channels.
Deformation and Force Chain of Two-Dimensional Granular Systems under Continuous Loading

A continuous loading experiment of a two-dimensional granular system was carried out and the experimental data were obtained by digital image correlation (DIC). The deformation field of the granular system and the changing laws of the deflection angle and coordination number of the granules on force chains with time were obtained. Based on the granule element method, the quantitative calculation of contact force was realized, and the internal force chains of the granular system were identified. The effects of contact force between granules and mechanical parameters on the evolution of force chains in a two-dimensional granular system under line loads were analyzed. The formation, evolution, and reconstruction of force chains in a granular system during loading, as well as the influence of the force chain network evolution on the macroscopic mechanical properties of granules were discussed. The experimental results indicated that the evolution of force chains was directly related to the number, geometric properties, and permutation distribution of granules in direct contact with the external load.
Analysis of Cell Motility with a Successive Sequence of Images

Analysis of cell motility usually requires several hours of continuousvideo recording of a single view field. Consequently, the method is very much timeconsuming and only a small number of cells is usually included into analysis. To increase the number of cells monitored in an experiment we developed a simpleand expeditious method for analysing the cell motility. A combination of successive series of images of individual cells relocated on CELLocate coverslips andvector graphic editing software Adobe Illustrator 9.0 enabled simultaneous monitoring of locomotion for up to ten times more cells as with previous methods.
Association of physical activity combined with sedentary behavior with dynapenia in older adults

This research aimed to analyze the association of physical activity (PA) level combined with exposure to sedentary behavior (SB) with dynapenia in older people. Epidemiological, cross-sectional study, conducted with 208 older adults in Aiquara, Bahia, Brazil. Habitual PA and SB were quantified using the International Physical Activity Questionnaire. Based on this information, participants were divided into groups (G): G1) sufficiently active and low SB; G2) sufficiently active and high SB; G3) insufficiently active and low SB; and G4) insufficiently active and high SB. Assessment of dynapenia was performed based on the values of the handgrip strength measured in kilogram-force (kgf) (women: 18.37 kgf; men: 26.75 kgf). Descriptive analysis was conducted using absolute and relative frequencies, mean and standard deviation. For the inferential analysis, Poisson regression was used, with a robust estimator, calculation of Prevalence Ratios (PRs) and their relevant Confidence Intervals (CIs) of 95.0%. It was observed that the mean age of women and men were, respectively, 71.0 ± 6.7 and 72.3 ± 8.1 years and the prevalence of dynapenia observed was about 24.5%. Furthermore, it was found that the insufficiently active older adults with low SB (G3) (PR: 2.28; 95%CI: 1.09-4.76), and those insufficiently active with high SB (G4) (PR: 4, 14; 95%CI: 1.95-8.70), were more likely to experience the assessed outcome. Evidence showed that, among the older adults in Aiquara the prevalence of dynapenia was high in the presence of an insufficient level of PA, especially when combined with greater exposure to SB.
Application of piezoelectric materials in the field of bone: a bibliometric analysis

In the past 4 decades, many articles have reported on the effects of the piezoelectric effect on bone formation and the research progress of piezoelectric biomaterials in orthopedics. The purpose of this study is to comprehensively evaluate all existing research and latest developments in the field of bone piezoelectricity, and to explore potential research directions in this area. To assess the overall trend in this field over the past 40 years, this study comprehensively collected literature reviews in this field using a literature retrieval program, applied bibliometric methods and visual analysis using CiteSpace and R language, and identified and investigated publications based on publication year (1984–2022), type of literature, language, country, institution, author, journal, keywords, and citation counts. The results show that the most productive countries in this field are China, the United States, and Italy. The journal with the most publications in the field of bone piezoelectricity is the International Journal of Oral & Maxillofacial Implants, followed by Implant Dentistry. The most productive authors are Lanceros-Méndez S, followed by Sohn D.S. Further research on the results obtained leads to the conclusion that the research direction of this field mainly includes piezoelectric surgery, piezoelectric bone tissue engineering scaffold, manufacturing artificial cochleae for hearing loss patients, among which the piezoelectric bone tissue engineering scaffold is the main research direction in this field. The piezoelectric materials involved in this direction mainly include polyhydroxybutyrate valerate, PVDF, and BaTiO3.
Factors Influencing Interest in Shopee Pay Later Users in Bengkulu

Organizations are static tools or containers. The organization was founded as a container to achieve the goals that have been set. The objectives to be achieved in this study are: To find out whether the speed of approval, increased effectiveness, increased productivity, ability to pay and security simultaneously affect the interest of online loan users. The method used in collecting data for this research is a questionnaire. The method of data analysis in this study is the Multiple Linear Regression Research Instrument Test the Coefficient of Determination of the Hypothesis.Based on the results of this study it was concluded that there is an influence on the speed of loan approval (X1) on the interest in using Bengkulu online loans (Y) There is an effect of increasing effectiveness (X2) on the interest in using Bengkulu online loans (Y) There is an effect of increasing productivity (X3) on the interest in using loans online Bengkulu (Y) There is an effect of the ability to pay (X4) on the interest in using online loans Bengkulu (Y) There is a security effect (X5) on the interest in using online loans Bengkulu (Y) There is a simultaneous influence on speed of approval, increased effectiveness, increased productivity , the ability to pay and security for the interests of online loan users.
Monitoring Land Use Dynamics in Chanthaburi Province of Thailand Using Digital Remotely Sensed Images

A comprehensive method of image classification was developed for monitoring land use dynamics in Chanthaburi Province of Tailand. RS (Remote Sensing), GIS (Geographical Information System), GPS (Global Positioning System) and ancillary data were combined by the method which adopts the main idea of classifying images by steps from decision tree method and the hybridized supervised and unsupervised classification. An integration of automatic image interpretation, ancillary materials and expert knowledge was realized. Two subscenes of Landsat 5 Thematic Mapper (TM) images of bands 3, 4 and 5 obtained on December 15, 1992, and January 17, 1999, were used for image processing and spatial data analysis in the study. The overall accuracy of the results of classification reached 90%, which was verified by field check. Results showed that shrimp farm land, urban and traffic land, barren land, bush and agricultural developing area increased in area, mangrove, paddy field, swamp and marsh land, orchard and plantation, and tropical grass land decreased, and the forest land kept almost stable. Ecological analysis on the land use changes showed that more attentions should be paid on the effect of land development on ecological environment in the future land planning and management.