Miracle-dz/UniFAR-FTUs huggingface dataset:
Biochemical and Genetic Analysis of the Vaccinia Virus D5 Protein: Multimerization-Dependent ATPase Activity Is Required To Support Viral DNA Replication (cid:1)

The vaccinia virus-encoded D5 protein is an essential ATPase involved in viral DNA replication. We have expanded the genotypic and phenotypic analysis of six temperature-sensitive ( ts ) D5 mutants (C ts 17, C ts 24, E ts 69, D ts 6389 [also referred to as D ts 38], D ts 12, and D ts 56) and shown that at nonpermissive temperature all of the ts D5 viruses exhibit a dramatic reduction in DNA synthesis and virus production. For C ts 17 and C ts 24, this restriction reﬂects the thermolability of the D5 proteins. The D ts 6389, D ts 12, and D ts 56 D5 proteins become insoluble at 39.7°C, while the E ts 69 D5 protein remains stable and soluble and retains the ability to oligomerize and hydrolyze ATP when synthesized at 39.7°C. To investigate which structural features of D5 are important for its biological and biochemical activities, we generated targeted mutations in invariant residues positioned within conserved domains found within D5. Using a transient complementation assay that assessed the ability of D5 variants to sustain ongoing DNA synthesis during nonpermissive C ts 24 infections, only a wt D5 allele supported DNA synthesis. Alleles of D5 containing targeted mutations within the Walker A or B domains, the superfamily III helicase motif C, or the AAA (cid:1) motif lacked biological competency. Furthermore, puriﬁed preparations of these variant proteins revealed that they all were defective in ATP hydrolysis. Multimerization of D5
Low-Carbohydrate-Diet Scores and Long-term Risk of Type 2 Diabetes Among Women With a History of Gestational Diabetes: A Prospective Cohort Study

OBJECTIVE Low-carbohydrate diets (LCDs) may improve short-term glycemic control in patients with gestational diabetes mellitus (GDM), but the long-term effect on progression from GDM to type 2 diabetes mellitus (T2DM) is unknown. We aimed to examine the long-term risk of T2DM in association with a low-carbohydrate dietary pattern among women with a history of GDM.
OPTIMIZING GREEN ITINERARY FOR SILVER NANOPARTICLES USING BOX-BEHENKEN EXPERIMENTAL DESIGN

Silver nanoparticles by virtue of stronger optical absorption have facilitated the development of biomedical sciences and engineering. The green bio-reduction approach was followed for synthesizing silver nanoparticles. Box-Behenken experimental design was employed as statistical method for optimization of synthetic experimental design. The method was optimized with respect to wavelength maxima obtained from UV-Vis spectroscopy analysis as a response. Analysis of variance (ANOVA) showed that silver nanoparticles could be effectively synthesized by tuning significant parameters of greener route.
Feasibility study of ambient cured geopolymer concrete –A review

Geopolymer concrete is a fastest developing field of research for utilizing industrial and agro waste materials as an alternative for Portland cement based concrete. Geopolymers are formed by the alkaline activation of aluminosilicates rich materials termed as geopolymerization. The process of geopolymerization requires elevated temperature curing which restricts its application to precast industry. This review summarizes the work carried out on developing the geopolymer concrete with the addition of various mineral admixtures at ambient curing temperature conditions. An overview of studies promoting the geopolymer concrete in general building construction is presented. Literature study revealed that geopolymer concrete with the addition of admixtures can exhibit desirable properties at ambient temperature conditions.
Extracorporeal membrane oxygenation for 2009 in ﬂ uenza A (H1N1) acute respiratory distress syndrome: single-centre experience with 1-year follow-up †

OBJECTIVES : During 2009, pandemic in ﬂ uenza A (H1N1) affected France and several patients developed in ﬂ uenza A (H1N1)-asso-ciated acute respiratory distress syndrome. The use of extracorporeal membrane oxygenation (ECMO) could be advocated as therapeutic solution. We present our experience with ECMO utilized in patients with in ﬂ uenza A (H1N1)-associated respiratory failure. METHODS : We conducted a retrospective observational analysis of our experience with veno-venous ECMO for 2009 in ﬂ uenza A (H1N1)-associated respiratory failure. We have excluded from our study all not con ﬁ rmed cases of in ﬂ uenza A (H1N1). Veno-venous ECMO was always instituted using a percutaneous cannulation technique. Mechanical circulatory support was maintained until respiratory function recovery. RESULTS : Between October 2009 and February 2010, we performed veno-venous ECMO support in 12 patients with in ﬂ uenza A (H1N1)-associated respiratory failure. Mean age was 33 ± 12 years (14 – 63 years) and there was a prevalence of female sex. Median time from in ﬂ uenza A (H1N1) onset to mechanical ventilation (MV) initiation was 6 days (1 – 17 days); median time from MV to veno-venous ECMO support was 3 days (1 – 20 days). Six patients (50%) suffered ventilator-associated pneumonia during ECMO support. Eight patients (66.6%) suffered signi ﬁ cant haemorrhage requiring transfusion of more than 2 packed red cells. In two patients (16.6%), there was a thrombosis of the inferior vena cava and one of them experienced pulmonary embolism. Mean duration of ECMO support was 23 ± 14 days (3 – 47 days); mean duration of mechanical ventilatory support was 24 ± 21 days (6 – 70 days). ECMO was weaned in 10 patients (83.3%) and all these patients are still alive after a period of follow-up of 13.8 ± 1.12 months (11.03 – 14.83 months). Two patients (in-hospital mortality of 16.6%) died under ECMO support for refractory septic shock. CONCLUSIONS : Veno-venous ECMO for 2009 H1N1-associated respiratory failure gives good results with a very low mortality rate. The use of a mobile unit is a safe procedure and may improve survival of patients who might not be otherwise eligible for transfer to our institution. Larger studies are however required in order to optimize and re ﬁ ne the best treatment strategy in this subgroup of patients.
Dynamics and stability of gravity-capillary solitary waves

Over the past several years, it has been recognized that a new class of solitary waves can propagate in nonlinear dispersive wave systems if the phase speed of linear waves attains a local extremum at some finite wavenumber. Near such a point, solitary waves in the form of small-amplitude wavepackets can be obtained for which the phase speed of the carrier oscillations matches the group speed of their envelope. Such an extremum is found in the analysis of water waves when the restoring forces of both gravity and surface tension are taken into account, and certain kinds of these gravity-capillary solitary waves have been observed in experiments. While past theoretical studies have focussed mainly on determining steady solitary wave profiles, very little work has been done on examining their stability properties which is the thrust of this thesis. Beginning in the weakly nonlinear regime, an asymptotic analysis of linear stability is presented and comparison is made with numerical computations. Contrary to predictions of the nonlinear Schr6dinger (NLS) equation, some free solitary wave types are found to be unstable owing to exponentially effects terms that lie beyond standard two-scale perturbation theory. Moreover, numerical simulations show that unstable gravity-capillary solitary waves may decompose into stable solitary waves that have soliton properties. Stability results are then extended to the fully nonlinear regime to treat both free and forced situations using numerical techniques to solve the full hydrodynamic equations in steady form. A dramatic difference is found between the linear stability of free and forced waves in both weakly and fully nonlinear cases, and results obtained here are compared with laboratory experiments. The analysis followed in the free-surface problem is then generalized to examine the dynamics of gravity-capillary interfacial solitary waves in a layered two-fluid system. Here, the linear stability and limiting wave forms of free solitary waves are determined over a range of system parameters using the full hydrodynamic equations. Finally, a related problem of gravity-capillary envelope solitons is considered under the general situation of unequal phase and group speeds. By asymptotic and numerical techniques it is found that envelope solitons are generally nonlocal-tails are radiated owing to a resonance mechanism that is beyond the NLS equation.
FOURIER TRANSFORM-INFRARED SPECTROSCOPY TO MONITOR MODIFICATIONS IN CANOLA BIOCHEMISTRY CAUSED BY ALTERNARIA DESTRUENS

Alternaria destruens (Simmons) is a serious pathogen of Brassica napus L. as well as the other members of mustard family resulting in significant yield loss worldwide. Fourier transform-infrared spectroscopy is a new, rapid, easy technique to study the compositional and metabolic changes in the plant cell wall after any biotic or abiotic stresses. In current research, FTIR technique was utilized to observe the biochemical changes in canola caused by A. destruens . The mid-infrared region of the spectrum revealed dramatic changes in protein, lipid and carbohydrate composition of the infected leaves and stem when compared to healthy leaves. Disturbed biochemistry of diseased plants could lead to a disaster for the yield of this major oil producing crop, therefore effective management strategy should be adopted on emergency basis.
Where Next for Microbiome Research? The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters

The development of high-throughput sequencing technologies has transformed our capacity to investigate the composition and dynamics of the microbial communities that populate diverse habitats. Over the past decade, these advances have yielded an ava-lanche of metagenomic data. The current stage of “ van Leeuwenhoek ”– like cataloguing, as well as functional analyses, will likely accelerate as DNA and RNA sequencing, plus protein and metabolic profiling capacities and computational tools, continue to improve. However, it is time to consider: what ’ s next for microbiome
INEQUALITIES FOR THE ＄r＄ -METRIC

Summary. Matusita’s r-metric on the set of probability measures on a measurable space is extended to the set of signed measures. and $r=2$ which coincide with the total variation and Hellinger metric respectively. Inequalities between these metrics are presented. As a consequence it is obtained that the topologies induced by the total variation norm and the r-metric are equivalent.
A lattice calculation of B -> K(*) form factors

Lattice QCD can contribute to the search for new physics in b -> s decays by providing first-principle calculations of B -> K(*) form factors. Preliminary results are presented here which complement sum rule determinations by being done at large q^2 and which improve upon previous lattice calculations by working directly in the physical b sector on unquenched gauge field configurations.
ADVANCES IN ACUTE MYELOID LEUKEMIA Epigenetics and approaches to targeted epigenetic therapy in acute myeloid leukemia

Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults. AML is a heterogeneous malignancy characterized by distinct genetic abnormalities. Recent discoveries have highlighted an additional important role of dysregulated epigenetic mechanisms in the pathogenesis of the disease. In contrast to genetic changes, epigenetic modifications are fre-quentlyreversible,whichprovidesopportu- nities for targeted treatment using specific inhibitors. In this review, we will provide an overview of the current state of epigenetics and epigenetic therapy in AML and will describe perspectives on how to identify promising new approaches for epigenetic targeted treatment. ( Blood . 2016;127(1): 42-52)
(cid:2) -Lactamase-Mediated (cid:2) -Lactam Resistance in Campylobacter Species: Prevalence of Cj0299 ( bla OXA-61 ) and Evidence for a Novel (cid:2) -Lactamase in C . jejuni (cid:1)

Fifty-two percent of 1,288 poultry isolates of campylobacters were ampicillin resistant, and resistance was more common among Campylobacter coli isolates (67.4%) than among Campylobacter jejuni isolates (47.5%). Production of (cid:1) -lactamase was typically associated with resistance to ampicillin, amoxicillin (amoxicilline), penicillin, and ticarcillin. Regardless of (cid:1) -lactamase production, all isolates were resistant to piperacillin (MICs > 256 (cid:2) g/ml), and most were resistant to carbenicillin, cloxacillin, and cephalosporins. Of all ampi-cillin-resistant campylobacters tested, 91% (347/380) carried the bla OXA-61 gene, and 77% (136/175) of those tested with nitroceﬁn produced a (cid:1) -lactamase, presumably OXA-61. The isoelectric point (pI) of OXA-61 was 8.7, and the molecular mass was 31.0 kDa. Insertional inactivation of bla OXA-61 in C . jejuni NCTC 11168 and two ampicillin-resistant isolates resulted in increased susceptibility to ampicillin, co-amoxiclav (amoxicillin and clavulanic acid), penicillin, carbenicillin, oxacillin, and piperacillin, but the effects on MICs of cephalo- sporins and imipenem were negligible. Some C . jejuni isolates that lacked bla OXA-61 produced a (cid:1) -lactamase, CjBla2, with a pI of 9.2 and molecular mass of 32.4 kDa. Mass spectrometry conﬁrmed that the most prevalent (cid:1) -lactamase was the product of bla OXA-61 , but CjBla2 was not identiﬁed. OXA-61 is prevalent among Campy- lobacter spp. of veterinary origin and is similar to the (cid:1) -lactamase previously reported in human isolates. was associated resistance to but not cephalosporins. Co-amoxiclav re- mained active against all isolates tested.
The salinity tolerance of pomegranate cultivars: Eﬀects of salt stress on root and leaf mineral content

: In this study, the eﬀect of irrigation water salinity on rooted cuttings of eight pomegranate cultivars namely ‘Malase Saveh’ (‘M­Saveh’), ‘Malase Isfahan’ (‘M­Isfahan’), ‘Robabe Ghermeze Shiraz’ (‘Robab’), ‘Gabrie Yazd’ (‘G­ Yazd’), Gabrie Torshe Yazd’ (‘GT­Yazd’), ‘Zaghe Seﬁde Yazd’ (‘ZS­Yazd’), ‘Zaghe Torshe Yazd’ (‘ZT­Yazd’) and ‘Malase Torshe Pishva’ (‘M­Pishva’) was studied. Sodium chloride was added to irrigation water to get ﬁnal concentrations of 3, 6, 9 and 12 dS/m. Leaf and root mineral content, leaf abscission and root char­ acteristics were determined at the end of the experiment. The results showed that the salinity reduced signiﬁcantly fresh and dry weight of root in all pome­ granate cultivars. Water salinity up to 3 dS/m increased slightly roots fresh and dry weight of all cultivars and thereafter decreased. With increasing water salinity to 12 dS/m, accumulation of sodium and chloride both in roots and leaves increased, but nitrogen, phosphorus, magnesium and calcium contents decreased. The change of leaf potassium content was dependent to pomegran­ ate cultivar. The lowest sodium and chloride accumulation in root was observed in ‘ZT­Yazd’, but ‘M­Pishva’ translocated the lowest sodium and chlo­ ride to leaf. The low ability of nitrogen absorption was found in ‘M­Saveh’, whereas ‘M­Pishva’ maintained the highest leaf nitrogen under salt stress con­ ditions. The most potassium content of root was observed in GT­Yazd’, while ‘ZS­Yazd’ and ‘G­Yazd’ translocated the highest potassium to leaves. Generally, the responses of pomegranate to absorption and translocation of elements to leaves under salinity conditions were completely dependent to cultivar. ‘M­ Pishva’ and Yazd cultivars showed higher tolerance to salinity stress.
Rapid Rise of Cardio-Ankle Vascular Index May Be a Trigger of Cerebro-Cardiovascular Events: Proposal of Smooth Muscle Cell Contraction Theory for Plaque Rupture

: Cardiovascular diseases have been recognized as the main cause of death all over the world. Recently, the established cardio-ankle vascular index (CAVI) has become known as an index of arterial stiffness of the arterial tree from the origin of the aorta to the ankle. CAVI reflects the progress of arteriosclerosis, and a rapid rise in CAVI indicates arterial smooth muscle cell contraction. Considering the vasculature of the atheroma where vasa vasorum penetrates the smooth muscle cell layer and supplies blood to the intimal atheromatous lesion, a rapid rise of CAVI means “choked” atheroma. Thus, we proposed a “smooth muscle cell contraction” hypothesis of plaque rupture.
Do Prices Determine Vertical Integration? The made this article openly available. Please share how this access benefits you. Your story matters

What is the relationship between product prices and vertical integration? While the literature has focused on how integration aﬀects prices, this paper provides evidence that prices can aﬀect integration. Many theories in organizational economics and industrial organization posit that integration, while costly, increases productivity. It follows from ﬁrms’ maximizing behavior that higher prices induce more integration. The reason is that at low prices, increases in revenue resulting from enhanced productivity are too small to justify the cost, whereas at high prices the revenue beneﬁt exceeds the cost. Trade policy provides a source of exogenous price variation to assess the validity of this prediction: higher tariﬀs should lead to higher prices and therefore to more integration. We construct ﬁrm-level indices of vertical integration for a large set of countries and industries and exploit cross-section and time-series variation in import tariﬀs to examine their impact on ﬁrm boundaries. Our empirical results provide strong support for the view that output prices are a key determinant of vertical integration. Dependent variable: log of (one plus) the absolute diﬀerence between countries c and c (cid:48) in the estimated vertical integration index of ﬁrms with primary sector k . The variable Diﬀerence Tariﬀs k,c,c (cid:48) is the diﬀerence between the MFN tariﬀ imposed by country c and c (cid:48) in sector k . Capital Intensity k is the total capital expenditures divided by value added. Diﬀerence Financial Development cc (cid:48) measures diﬀerences in private credit by deposit money banks and other ﬁnancial institutions as a fraction of GDP. The variable Diﬀerence in Legal Quality c proxies diﬀerences in the quality of institutions across between two countries. The variables Contiguity c,c (cid:48) , Colonial Relationship c,c (cid:48) , Common Language c,c (cid:48) and Diﬀerence GDP c,c (cid:48) capture bilateral geographical, cultural and economic linkages.
‘ ‘ A KEY DIFFERENCE BETWEEN PREDICTIVE MODELING FOR PROFESSIONAL RACING COMPARED TO THAT IN BASKETBALL ( AND BASEBALL ) IS THE NATURE OF THE EVOLUTION OF THE GAME

Our goal is to design a prediction and decision system for real-time use during a professional car race. In designing a knowledge discovery process for racing, we faced several challenges that were overcome only when domain knowledge of racing was carefully infused within statistical modeling techniques. In this article, we describe how we leveraged expert knowledge of the domain to produce a real-time decision system for tire changes within a race. Our forecasts have the potential to impact how racing teams can optimize strategy by making tire-change decisions to benefit their rank position. Our work significantly expands previous research on sports analytics, as it is the only work on analytical methods for within-race prediction and decision making for professional car racing.
Complex projective structures: Lyapunov exponent, degree, and harmonic measure

We study several new invariants associated to a holomorphic projective structure on a Riemann surface of finite analytic type: the Lyapunov exponent of its holonomy which is of probabilistic/dynamical nature and was introduced in our previous work; the degree which measures the asymptotic covering rate of the developing map; and a family of harmonic measures on the Riemann sphere, previously introduced by Hussenot. We show that the degree and the Lyapunov exponent are related by a simple formula and give estimates for the Hausdorff dimension of the harmonic measures in terms of the Lyapunov exponent. In accordance with the famous "Sullivan dictionary", this leads to a description of the space of such projective structures that is reminiscent of that of the space of polynomials in holomorphic dynamics.
AN ASSOCIATIVE STUDY OF NON-LIPID RISK FACTORS (HIGH SENSITIVE-CRP, URIC ACID AND THYROID STIMULATING HORMONE) WITH PARAMETERS OF TYPE 2 DIABETES MELLITUS, BLOOD PRESSURE IN METABOLIC SYNDROME

Background : Metabolic syndrome is the huddle of diseases for atherosclerotic cardiovascular disease (ASCVD) which arises due to excess of plasma glucose, cholesterol, fatty acids, blood pressure and obesity. The role of lipids in the development of MetS had been extensively studied. Some non-lipid factors like hsCRP, uric acid and TSH level also remain elevated in the serum of the MetS patients the correlation of the same with the MetS not fully determined. Aim and Objectives : Hence in this study our aim and objectives was to assess the significance of non-lipid risk factors in determining the severity of MetS with the association of type 2 diabetes and blood pressure parameters. Methods : A total of 450 subjects (211 men and 239 women) aged ≥ 35 years attending the hospital were divided based on the components of MetS as control (CS), Metabolic syndrome (MS) and severe (SMS) MetS groups. Comparative study was done by one way ANOVA and variables with significant associations were included in regression analysis to determine the future prediction with non-lipid risk factors hs-CRP, serum uric acid and TSH as dependent variables. The cardiovascular and T2DM related parameters FPG, HbA1c, serum fasting insulin, HOMA-IR, SBP, DBP, PR were independent variables in this study. Results : All the T2DM and BP variables had highly significant relation (P<0.001) when compared MetS and severe Mets group with control group. In this study, the value of participants HbA1C, PR, FPG, DBP and IR had significant Adjusted OR [3.03 P<0.001; 2.10 P 0.004; 1.86 P 0.033; 0.17, P <0.001 and 0.08, P <0.001 respectively] high positive correlation with the hs-CRP values. It was found that HbA1c, SBP, DBP, FPG and PR had significant predictive AOR [5.829 P<0.001; 2.789 P 0.007; 0.098 P<0.001; 0.383 P 0.008 and 0.543 P 0.053 respectively] in association with Uric acid values. Also, PR, FPG and DBP had significant AOR significance [1.96 P 0.001; 1.41 P <0.001; 1.37 P 0.026 respectively] in association with TSH values. Conclusion: It can be concluded that reducing the above T2DM and cardiovascular variables by various lifestyles or other means can reduce the levels of non-lipid risk factors and thereby arrest susceptibility of the development of MetS and severe MetS.
Connections and Extensions: A Discussion of the Paper by Girolami and Byrne

We welcome this paper of Byrne and Girolami [BG]; it breathes even more life into the emerging area of hybrid Monte Carlo Markov chains by introducing original tools for dealing with Monte Carlo simulations on constrained spaces such as manifolds. We begin our comment with a bit of history. Using geodesics to sample from the uniform distribution on Stiefel manifolds was proposed by Asimov (Asimov, 1985) in his work on the Grand Tour for exploratory data analysis. For data x 1 ;x 2 ;::: ;x n in R p , it is natural to inspect low-dimensional projections (cid:2)x 1 ;(cid:2)x 2 ;::: ;(cid:2)x n for (cid:2) W R p (cid:2)! R k . In the BG paper, the authors have a space of k-frames in R p , called V k;p . If one chooses (cid:2) at random from this space, the views would be too ‘disconnected’ or ‘jerky’ for human observers. A better tactic turned out to be to choose a few (cid:2) i ;1 (cid:3) i (cid:3) L , at random and then moving smoothly from (cid:2) i to (cid:2) j by available closed form geodesics. While in a historical mode, we point to the little known papers of McLachlan (McLachlan & Quispel, 2003) and more recent papers by Betancourt on hybrid Monte Carlo (Betancourt, 2013).