Miracle-dz/UniFAR-FTUs huggingface dataset:
Design, Synthesis, and Biological Evaluation of Triazolyl- and Triazinyl-Quinazolinediones as Potential Antitumor Agents

Novel 6(3-1H-1,2,4-triazol-1-yl)-3-phenylquinazoline-2,4(1H,3H)-diones (7a–e) were synthesized from different enaminones (6a–e) with 6-hydrazinyl-3-phenylquinazoline-2,4(1H,3H)-dione. 2,6(4-2-Substituted-1,3,5-triazin-1(2H)-yl)-3-phenylquinazoline-2,4(1H,3H)-diones (8a–k) were synthesized from the reaction of 1-(2,4-dioxo-3-phenyl-1,2,3,4-tetrahydroquinazolin-6-yl)thiourea, urea, or guanidine (3a–c) with enaminones (6a–e), and a series from 3-substituted-2-imino-1,3,5-triazin-1(2H)-yl-sulfonyl-phenyl-1-methylquinazoline-2,4(1H,3H)-dione (12a–j) were obtained from the reaction of N-(diaminomethylene)-4-(1-methyl-2,4-dioxo-1,2-dihydroquinazolin-3(4H)-yl)benzenesulfonamide (11) with the enaminone (6a–j). The antitumor activity of the synthesized compounds was evaluated against two human cell lines: human colon carcinoma HCT116 and human hepatocellular carcinoma HEP-G2. Some of the tested compounds showed significant potency compared to the reference drug staurosporin.
Neuropharmacological basis for multimodal analgesia in chronic pain

ABSTRACT Managing chronic pain remains a major unmet clinical challenge. Patients can be treated with a range of interventions, but pharmacotherapy is the most common. These include opioids, antidepressants, calcium channel modulators, sodium channel blockers, and nonsteroidal anti-inflammatory drugs. Many of these drugs target a particular mechanism; however, chronic pain in many diseases is multifactorial and induces plasticity throughout the sensory neuroaxis. Furthermore, comorbidities such as depression, anxiety, and sleep disturbances worsen quality of life. Given the complexity of mechanisms and symptoms in patients, it is unsurprising that many fail to achieve adequate pain relief from a single agent. The efforts to develop novel drug classes with better efficacy have not always proved successful; a multimodal or combination approach to analgesia is an important strategy in pain control. Many patients frequently take more than one medication, but high-quality evidence to support various combinations is often sparse. Ideally, combining drugs would produce synergistic action to maximize analgesia and reduce side effects, although sub-additive and additive analgesia is still advantageous if additive side-effects can be avoided. In this review, we discuss pain mechanisms, drug actions, and the rationale for mechanism-led treatment selection. Abbreviations: COX – cyclooxygenase, CGRP – calcitonin gene-related peptide, CPM – conditioned pain modulation, NGF – nerve growth factor, NNT – number needed to treat, NMDA – N-methyl-d-aspartate, NSAID – nonsteroidal anti-inflammatory drugs, TCA – tricyclic antidepressant, SNRI – serotonin-noradrenaline reuptake inhibitor, QST – quantitative sensory testing.
Тhe content of calcium and phosphorus in the blood of cows with a different tonus of the autonomic nervous system

Research was conducted on cows of the Ukrainian black-and-white breed of the 2–3 lactation. Tonus of the autonomic nervous system (ANS) of cows was determined using trigeminovagal reflex (TVR). According to the obtained results, an animal was designate as normotonics, sympatheticotoniсs or vagotonics. According to the results of the study of the ANS tonus, 3 experimental groups were formed 4 animals in each. The first group included animals-normotonics, the second – vagotonics, and the third one – sympatheticontics. It was established a tendency of сalcium/hosphorus lower content in the blood of cows during the warm season and higher in the winter (in the range of 3–8%). It should also be noted that there was tendency towards lower calcium content in the blood of vagotonic cows and higher – in sympatheticotoniс animals, regardless of the season. Unlike calcium, the content of phosphorus in the blood of cows significantly depended on the ANS tonus. In particular, the content of this element in the blood of sympatheticotoniс and vagotonic cows in winter was lower by 14.0% (P < 0.001) and 18.3% (P < 0.001), respectively. However, in the summer, this difference became significantly smaller and had character of a trend (at the level of 5.0–7.2%). It has been established that the calcium-phosphorus ratio in cow’s blood did not depend significantly on the ANS tonus or on the season of the year, but a clear trend was established for its lower value in summer, regardless of the ANS tonus. The strength of excitability levels of the sympathetic and parasympathetic nervous system according to the results of the TVR in cows did not significantly limit the phosphorus content in their blood in summer, and calcium in winter (η2x = 0.00–0.019). But in normotonic animals, the ANS tonus significantly affected the calcium-phosphorus ratio – η2x = 0.59 (P < 0.05) and the phosphorus content in their blood – η2x = 0.29 (P < 0.05) in winter. It was established that ANS tonus in sympatheticotonic animals limited the phosphorus content in winter – η2x = 0.35 (P < 0.05) and the calcium-phosphorus ratio as in summer – η2x = 0.29 (P < 0.05) so in winter – η2x = 0.45 (P < 0.05). Whereas in vagotonic cows, the ANS tonus limited the calcium content in the summer – η2x = 0.26 (P < 0.05). Correlation analysis of the ANS tonus index, phosphorus and calcium content in cow’s blood did not establish significant interrelationships depending on the season of the year. However, it should be noted a clear trend of direct correlation of the index of the ANS tonus with the calcium content (r = 0.24–0.35) and the calcium-phosphorus ratio (r = 0.43–44) in the cows’ blood and the reverse one with the phosphorus content in the cows’ blood (r = -0.22–0.30). The data obtained could indicate on the presence of autonomic regulatory mechanisms for the regulation of the content of specific macronutrients in the blood of cows.
Factors determining intention to vaccinate against Covid-19: A developing nation perspective

Abstract India is more challenged than developed nations in reducing the Covid-19 cases and lowering death rates due to the huge population and unprepared health care system. As intention to vaccinate is contextual and its availability does not translate into their de facto adoption. , this paper examines the factors that determine intention to vaccinate against COVID-19 in India. A research model was tested using 222 responses was tested using structural equation modeling. Findings suggested that credibility and awareness positively impacted whereas vaccine hesitancy and perceived risk negatively influenced intention to vaccinate. The fear of side effects did not impact the intention to vaccinate.
Preliminary design of corncob based furfural plant

Cob corn is one of solid waste farm which contents pentosan, and has economic value to be produced as furfural products. Furfural making process use semibatch process by generating hydrolysis main reaction with sulfuric acid catalyst. Reactor in use is stirred batch reactor under 2 bar pressure and 128°C for 70 minutes. Furfural purification use azeotropic distillation and dehydration column to gain furfural with 99% purity. Suprayield Technology is chosen due its better economic value to be compared with conventional technology. According to SuperPro Designer® simulation, to produce 183 kg/batch furfurals based on 2008’s market demands expectation, needs 900 kg/batch (4.9 kg/batch furfural) raw materials in form of cob corn, 36% sulfic acid or in amount of 84 kg/batch (0.45 Kg/Kg furfural). For production capacity of 362 tons per year, to build a furfural fabric in Indonesia demand US$ 2.855. 773,00 total investment, with manufacturing cost about US$ 189.865,00. The proper parameter of furfural fabric with 362 tons per annum is within NPV US$ 2.873. 820,29, IRR 15%, PBP 4 years and 9 months.Cob corn is one of solid waste farm which contents pentosan, and has economic value to be produced as furfural products. Furfural making process use semibatch process by generating hydrolysis main reaction with sulfuric acid catalyst. Reactor in use is stirred batch reactor under 2 bar pressure and 128°C for 70 minutes. Furfural purification use azeotropic distillation and dehydration column to gain furfural with 99% purity. Suprayield Technology is chosen due its better economic value to be compared with conventional technology. According to SuperPro Designer® simulation, to produce 183 kg/batch furfurals based on 2008’s market demands expectation, needs 900 kg/batch (4.9 kg/batch furfural) raw materials in form of cob corn, 36% sulfic acid or in amount of 84 kg/batch (0.45 Kg/Kg furfural). For production capacity of 362 tons per year, to build a furfural fabric in Indonesia demand US$ 2.855. 773,00 total investment, with manufacturing cost about US$ 189.865,00. The proper parameter of furfural fabric wit.
Vertical transmission of hepatitis B virus: propositions and future directions

Abstract Chronic hepatitis B virus (HBV) infection due to vertical transmission remains a critical concern with regards to eliminating HBV infection. Implementation of hepatitis B vaccine, the foundation to prevent perinatal and horizontal transmission, has reduced the prevalence of HBV by >80%. In countries where the hepatitis B immune globulin (HBIG) is available, such as China and the United States, the administration of HBIG and hepatitis B vaccine to the infants of mothers who are positive for hepatitis B surface antigen has become a standard practice and is effective in preventing vertical transmission. Accumulating evidence on the efficacy and safety of antiviral prophylaxis during pregnancy indicates the probability of attaining the goal of the World Health Organization to eliminate hepatitis by 2030. In this review, we discuss the transmission routes, diagnostic criteria, and preventive strategies for vertical transmission. A preventive program that includes screening before pregnancy, antiviral prophylaxis during pregnancy, and postpartum immunoprophylaxis provides “perfect strategies” to eliminate vertical transmission. However, there is still a notable gap between “perfect strategies” and real-world application, including insufficient coverage of timely birth dose vaccine and the efficacy and necessity of HBIG, especially in mothers who are negative for hepatitis B envelope antigen. In particular, there is a clear need for a comprehensive long-term safety profile of antiviral prophylaxis. Therefore, feasible and cost-effective preventive strategies need to be determined across regions. Access also needs to be scaled up to meet the demands for prophylaxis and prevalence targets.
Enhancing Phytoremediation Efficiency Using
Regulated Deficit Irrigation

In this study, Beta vulgaris L. var. cicla was grown in cadmium-contaminated soil in a greenhouse. Regulated deficit irrigation was applied using three different irrigation levels (T1: 300 L, T2: 200 L, T3: 100 L per block during each irrigation event during the organogenesis stage; T1 was the control) to examine the effects on phytoremediation efficiency. According to the experimental results, the regulated deficit irrigation treatment (T2) decreased the Beta vulgaris L. var. cicla shoot biomass by 15.8%, increased the Cd concentration in the shoots by 23%, and maintained a constant root-shoot ratio. By contrast, T3 decreased the Beta vulgaris L. var. cicla shoot biomass by 33.0%, decreased the Cd concentration in shoots by 9.8%, and increased the root-shoot ratio by 62.8%. The Cd remediation potential efficiency (PE) of treatment T2 was 5.42 g ha-1 – i.e., 39.7% higher than that of T1 and 61.8% higher than that of T3. This study indicated that regulated deficit irrigation can be used to enhance Cd phytoremediation and save water, but should be applied in a suitable way.
Assessing Global-Local Secondary Structure Fingerprints to Classify RNA Sequences With Deep Learning

RNA elements that are transcribed but not translated into proteins are called non-coding RNAs (ncRNAs). They play wide-ranging roles in biological processes and disorders. Just like proteins, their structure is often intimately linked to their function. Many examples have been documented where structure is conserved across taxa despite sequence divergence. Thus, structure is often used to identify function. Specifically, the secondary structure is predicted and ncRNAs with similar structures are assumed to have same or similar functions. However, a strand of RNA can fold into multiple possible structures, and some strands even fold differently in vivo and in vitro. Furthermore, ncRNAs often function as RNA-protein complexes, which can affect structure. Because of these, we hypothesized using one structure per sequence may discard information, possibly resulting in poorer classification accuracy. Therefore, we propose using secondary structure fingerprints, comprising two categories: a higher-level representation derived from RNA-As-Graphs (RAG), and free energy fingerprints based on a curated repertoire of small structural motifs. The fingerprints take into account the difference between global and local structural matches. We also evaluated our deep learning architecture with k-mers. By combining our global-local fingerprints with 6-mer, we achieved an accuracy, precision, and recall of 91.04%, 91.10%, and 91.00%.
Spontaneous spinal epidural haematoma: management and main risk factors in era of anticoagulant / antiplatelet treatment.

AIM OF THE STUDY Spontaneous spinal epidural haematomas (SSEH) are rare nosological units wherein acute collections of blood develop in the spinal canal. SSEH are usually manifested by sudden severe back pain accompanied by the development of neurological symptoms. In this study, we retrospectively describe management and the main risk factors of SSEH in a series of 14 cases. MATERIAL AND METHODS Between 2010 and 2019, we examined 14 patients (age range 17-89 years, 10 women) diagnosed with SSEH. Eight cases were patients using anticoagulant therapies (six warfarin, one dabigatran, one apixaban) and two others were using ASA of 100 mg/day. The exact localisation and extent of changes was determined from acute magnetic resonance imaging. Three people using warfarin had INR values higher than 3.0 at the time of their diagnosis. RESULTS Ten patients (71%) were taking oral anticoagulants or antiplatelet agents. In seven patients, SSEH were localised in the lower cervical/thoracic spine. Ten patients (71%) had arterial hypertension. Six patients underwent acute surgery due to rapidly developing spinal cord compression. Eight patients (57%) with slight or mild neurological symptoms were successfully managed without surgery. CONCLUSIONS SSEH should be suspected in any patient receiving anticoagulant/antiplatelet agents who complains of sudden, severe back pain accompanied by neurological symptoms. SSEH is mostly localised in the lower cervical/thoracic spine. Arterial hypertension appears to be a risk factor of SSEH. Early decompression is an important therapeutic approach; in cases with minor neurological deficits, conservative treatment may be chosen.
Different microbial responses in top‐ and sub‐soils to elevated temperature and substrate addition in a semiarid grassland on the Loess Plateau

The Loess Plateau soil in northwest China originated from wind sediments and is characterized by deep soil profiles and large organic carbon (C) content. Severe soil erosion constantly exposes deep soils to the surface, making the organic C vulnerable to microbial decomposition. Few, however, have so far examined how soil microbial activity and community composition in the deep loess soil respond to perturbations. We examined microbial responses in three layers of a clay‐loam loess (topsoil, 0–20 cm; midsoil, 40–60 cm; subsoil, 80–100 cm) to substrate additions (0.8 g glucose‐C kg−1 soil) under two temperature regimes (25 and 35°C). Soil C:N ratio was significantly larger in the subsoil (20.3) than topsoil (7.4). Glucose addition significantly increased CO2 efflux during a 30‐day incubation period and the relative magnitude of the increase was four times larger in the subsoil than topsoil. The temperature sensitivity (Q10) of soil CO2 efflux increased significantly with soil depth in the absence of glucose addition (i.e., ambient soil), but it decreased under glucose addition. Also, glucose addition significantly increased phenol oxidase and peroxidase activities in the subsoil, which might contribute to the stimulation of microbial CO2 efflux. Composition of the microbial community was more affected by temperature increase in the topsoil, but more responsive to labile C addition in the subsoil. Together, these results indicated that the composition of soil communities and microbial activities in the topsoil and deep soil responded differently to warming and labile C input. Our findings suggest that organic C in deep loess soils can be highly sensitive to environmental changes, emphasizing the need for more long‐term monitoring and quantitative assessment of organic C release from this important C pool.
Online Disclosure of Sexual Victimization: A Systematic Review

We map the available scientific literature on how and why victims of sexual violence use digital platforms in the aftermath of victimization. Twenty-four empirical studies on sexual victimization and online disclosure were identified by systematically searching Web of Science and PsycINFO, checking reference lists, and consulting authors about relevant publications. The literature on online disclosure of sexual victimization does not yield a coherent picture. International literature pays limited attention to the various components of online disclosure like the characteristics of victims who disclosure online and the characteristics of the disclosure messages. Most studies focused on motivations for and reactions to online disclosure. Victims of sexual violence disclose sexual victimization online to seek support for clarification and validation, unburdening, documenting, seeking justice, informing others, or commercial goals (individual-oriented disclosure) and to provide support, educate, and as a form of activism (other-oriented disclosure). Responses to online disclosure are predominantly positive. Negative responses are rare. This review provides a comprehensive overview of multidisciplinary empirical information and displays knowledge gaps in victimological research. Future research should use robust quantitative and/or qualitative designs with substantial sample sizes, comparing victims who do disclose their sexual victimization online to victims who do not and comparing disclosure on different online platforms to increase generalizability. Potential for online support is identified, in which online disclosure can serve as a relatively safe alternative to off-line disclosure. This offers points of intervention for assistance and victim support in facilitating the use of the internet for support for victims of sexual violence.
No overall impact on rate of weight gain with integrase inhibitor‐containing regimens in antiretroviral‐naïve adults

Integrase strand transfer inhibitors (INSTIs) are commonplace in modern antiretroviral therapy (ART). Increased weight gain with their use is increasingly scrutinized. We evaluated weight changes in treatment‐naïve adults with HIV‐1 attending a UK centre who started regimens including raltegravir or dolutegravir.
The Concept of Pain Inventory for Adults (COPI-Adult)

Supplemental Digital Content is available in the text. Objectives: Assessing knowledge and beliefs regarding pain science can identify gaps and misconceptions. The Concept of Pain Inventory (COPI) was recently developed in children with the intent to guide targeted pain science education. We utilized the original COPI item pool to (1) develop a tool to assess an adult’s concept of pain in a cohort who had not received pain science education, (2) evaluate its psychometric properties, (3) examine distribution of scores in a cohort of adults who had received pain science education, and (4) examine associations between scores and clinical variables. Materials and Methods: A total cohort of 627 adults were recruited through social media for an online survey. Initial development was conducted on those who had not received prior pain science education (n=125), then the COPI-Adult tool was tested in those who had received prior pain science education (n=502). Results: The resulting unidimensional 13-item COPI-Adult had acceptable internal consistency (α=0.78) and good test-retest reliability at 1 week (Intraclass Correlation Coefficient3,1=0.84 (95% confidence interval: 0.71-0.91). Higher COPI-Adult scores reflect greater alignment with contemporary pain science. COPI-Adult scores were correlated with revised Neurophysiology of Pain Questionnaire (rNPQ) scores and inversely correlated with average and current pain intensity, and pain interference. Adults who reported having received pain science education had significantly higher mean COPI-Adult scores than those who had not, and this difference exceeded the smallest detectable change. Discussion: The COPI-Adult is a brief questionnaire with promising psychometric properties to identify conceptual gaps or misconceptions to inform individualized pain science education.
Clinical Characteristics Between Survivors and Nonsurvivors of COVID-19 Patients Requiring Extracorporeal Membrane Oxygenation (ECMO) Support: A Systematic Review and Meta-Analysis

Background: The use of extracorporeal membrane oxygenation (ECMO) in coronavirus disease 2019 (COVID-19) for refractory respiratory failure, severe cardiac dysfunction, and bridge to lung transplantation has been steadily increasing during the ongoing global pandemic. Objective: Our meta-analysis aims to compare the clinical characteristics between COVID-19 survivors and nonsurvivors requiring ECMO support. Methods: A systematic search of Pubmed, Cochrane, Embase, Scopus, and Web of Science databases was performed between December first, 2019, to June first, 2021. Studies with comparative data of COVID-19 ECMO patients were selected, in which clinical characteristics and complications were assessed. Results: Sixteen cohort studies involving 706 COVID-19 patients requiring ECMO support with pooled mortality rate of 40% were included. Younger age (mean 51 years vs 55 years; P < .001), fewer comorbidities (23% vs 31%; odds ratio [OR] 0.55; P = .02), and less renal replacement therapy (RRT) (21% vs 39%; OR 0.41; P = .007) and vasopressor (76% vs 92%; OR 0.35; P = .008) requirement were demonstrated in COVID-19 survivors requiring ECMO support than nonsurvivors. Survivors also had higher pre-ECMO pH (mean 7.33 vs 7.26; P < .001) than nonsurvivors. No difference was observed in gender, body mass index, duration of mechanical ventilation (MV) before ECMO support initiation, total ECMO support duration, and pre-ECMO parameters of PaO2/FiO2 ratio, tidal volume (mL/kg), positive end-expiratory pressure, and plateau pressure. The rate of bleeding complications was lower in survivors (32% vs 59%; OR 0.36; P = .001) than nonsurvivors, but no difference was observed in thromboembolism and secondary infections. Conclusions: We found advanced age, multiple comorbidities, lower pre-ECMO pH, greater RRT, and vasopressor requirements, and bleeding are predictors of death in COVID-19 patients requiring ECMO support. The duration of MV before ECMO support initiation and total ECMO support duration was similar among survivors and nonsurvivors. Our study results have important clinical implications when considering ECMO support in critically ill COVID-19 patients.