Miracle-dz/UniFAR-FTUs huggingface dataset:
217 th ENMC International Workshop: RYR1 -related Myopathies, 29-31 st January 2016, The Netherlands

as well as subunits, and their respective targeting/anchoring in a macromolecular complex that exceeds three million Daltons. Andy have obtained high-resolution cryo-electron microscopy (Cryo-EM) reconstructions from highly purified rabbit skeletal muscle RyR1 in the open and closed states. These data reveal that RyRs are members of the six transmembrane family of ion channels and show a mechanism for channel gating that couples a change in the conformation of the Ca 2+ binding site directly to opening of the channel pore, suggesting that Ca 2+ binding facilitates mechanical coupling of conformational changes in the cytosolic region to opening of the channel gate. The channel-specific ligand ryanodine shows the channel to be locked in an open state consistent with the know effects of ryanodine on the single channel properties of RyR1. Their team have mapped >180 disease causing mutations on the structure proving insight regarding disease mechanisms for
Interactions between engineered nanomaterials and agricultural crops: implications for food safety

Engineered nanomaterials (ENMs) are being discharged into the environment and to agricultural fields, with unknown impacts on crop species. In this paper, we review the literature on ENMs uptake, translocation/distribution, and generational transmission in various crop species, as well as potential material trophic transfer. Previous studies reveal that ENM-exposed crops exhibit adaptive processes in response to stress, including endocytosis/endosome activities, production of antioxidant enzymes, regulation of genes related to cell division/extension and membrane transport. Some agronomic traits of crops are compromised during the adaption response, including photosynthesis, fruit yields, nutritional quality and nitrogen fixation. Cultivation of crops in ENMs-contaminated environments has unknown implications for food safety and quality. Notably, mechanisms underlying ENMs phytotoxicity and bioavailability are unclear. Additional investigations focused on developing novel techniques for in vivo identification/characterization of ENMs are critically needed. Given the abundance of uncertainty in the literature, it is clear that more research is urgently needed in the area of ENMs-crop interactions; only then can one accurately assess exposure, risk, and overall implications for food safety and also enable guidance development for the sustainable implementation of nanotechnology in agriculture and food production/manufacturing. 概要研究目的通过综述作物对纳米材料的吸收途径和积累, 以及纳米材料对农作物生长和营养的影响, 为纳米污染在农业中的风险提供理论分析和启示。 创新要点归纳了纳米材料被作物吸收的路径和对作物生理、 遗传、 营养各水平上产生的胁迫。 重要结论当前纳米与作物的研究应集中在食品安全相关的问题上, 考虑农业实际情况和环境因素, 分析纳米材料通过食物链富集和传递的可能性, 探讨纳米材料与其他土壤有机污染物可能产生的复合污染。
Ginkgo biloba extract-761 protects myocardium by regulating akt/nrf 2 signal pathway

Objective: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI). Materials and methods: Forty Sprague Dawley rats were randomly divided into following four groups: sham group, I/R group and EGb 761 groups (20 and 40 mg/kg). MIRI model was established after 14 days of administration. The myocardial infarct size and myocardial histology were measured and compared. Meanwhile, the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin T (TnT), TNF- α , IL-6, IL-1 β , superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were evaluated. Western blot was used to detect the expression of Caspase-3, Bax, Bcl-2, HO-1, Nrf2, Akt, p-Akt and nuclear protein Nrf2. Results: The levels of infarct size, CK-MB, LDH, TnT, TNF- α , IL-6 and IL-1 β in the EGb 761 groups were significantly lower than those in the ischemia/reperfusion (I/R) group. The content of MDA was lower in the myocardium, whereas the activities of SOD and GSH-Px were higher than those in the I/R group. The expressions of Caspase-3 and Bax in the EGb 761 groups were significantly lower than those in the I/R group, whereas the expressions of Bcl-2, p-Akt and HO-1 and nuclear protein Nrf2 in the EGb 761 groups were higher than those in the I/R group. Conclusion: EGb 761 might inhibit the apoptosis of myocardial cells and protect the myocardium by activating the Akt/Nrf2 pathway, increasing the expression of HO-1, decreasing oxidative stress and repressing inflammatory reaction. Some research studies indicated that PI3K–Akt signaling pathway played an important role in the expression of nuclear factor Nrf2 and downstream antioxidant gene in the antioxidation process. And the activities of GSH-Px and SOD were enhanced by the activated Nrf2 pathway, the oxidative stress and inflammatory response, the cell and tissue
Solid-state modeling and basic training of specialists in the field of mechanical engineering

One of the main elements of basic training for modern machine-building enterprises is modern 3D-modelling technology. Currently, it is not only the construction of solid-state models of mechanical systems, but also their support – accumulation and analysis of data for the working efficiency of a product during the entire period of its operation. The article is devoted to forming of the initial level of knowledge, experience and practical skills necessary to work with models designed to manage the products life cycle. Different methods of teaching 3D modeling in Autodesk software environment are also considered in the article. Attention is focused on the interrelation between these methods and traditional methods of construction.
Renewable energy assisted traffic aware cellular base station energy cooperation

With global concern for climate change, and for cutting down the energy cost, especially in off grid areas, use of renewable energy has been gaining widespread attention in many areas including cellular communication. The base station (BS) has emerged as a strong candidate for the integration of renewable energy sources (RES), particularly solar and wind. The incorporation of renewable energy opens many possibilities for energy conservation through strategies such as energy cooperation between BSs during the off-peak hours, when the energy harvested from renewable energy sources may become surplus. In this paper, we present the case for cellular BSs enabled with renewable energy sources (RES) to have an arrangement in which the BS provide surplus energy to a neighboring BS, thus minimizing the use of conventional energy. A realistic objective is developed for northern region of Pakistan, which entails modeling of solar panels and wind-turbine according to the average solar irradiation and wind speed of the region. We also model the dynamic load of the BS, which depicts temporal fluctuations with traffic variations. Based on these models we initiate an energy cooperation scheme between the BS in which an energy cost minimization framework is mathematically modeled and solved through the interior point method algorithm. Results are obtained for different times of the year for different number of base stations showing respective energy cost savings. Keywords: Base station Cooperation; alternate energy; cellular networks
The challenge of electromagnetic field measurements for modern 5G Devices

This overview paper shows the challenges for antenna measurements facing with 5G devices. The new antenna related technologies: higher frequency bands to millimeter bands, broadband, new technologies as distributed MIMO, massive MIMO, phased arrays, adaptive beamforming or the fact that the sensors are fully integrated to the telecommunication system makes a real challenge the characterization of the antennas themselves. The figure of merit of interest for the users will be much more complicated than the far field performance. In fact, much of these devices will be interconnected in near field, not only in far field, and basically the interest will come in the reduction of the bit error rate and the increase of the capacity. All these facts make necessary to use Over the Air measurement systems, increasing the measurement speed (real time) and increasing the accuracy for measuring the millimeter frequencies.
Relationship between Psychosocial Care Centers and Services in Primary Health Care

OBJECTIVE: To analyze forms of articulation that the Centers for Psychosocial Care (CAPS) established with the Primary Health Care Services. METHODS: A descriptive, qualitative study conducted using document review of 23 Therapeutic Projects of the CAPS type I and II, of 23 cities in the southern region of Brazil (PR, SC and RS). RESULTS: It was observed that the structure of articulation occurs basically through the supervision and training of teams, and a reference and counter-reference system, often masked under the logic of the referrals. However, these actions are more suggestive, referred to in documents as objectives, than they are properly translated into actions and concrete operational logic of those in daily practice services. CONCLUSION: In fact, attention to the complexity of the demand for mental health is dependent on a gradual construction of joint responsibility guided by the various services that comprise the network of care.
Inactivation of the Osteopontin Gene Enhances Vascular Calciﬁcation of Matrix Gla Protein–deﬁcient Mice: Evidence for Osteopontin as an Inducible Inhibitor of Vascular Calciﬁcation In Vivo

Osteopontin (OPN) is abundantly expressed in human calcified arteries. To examine the role of OPN in vascular calcification, OPN mutant mice were crossed with matrix Gla protein (MGP) mutant mice. Mice deficient in MGP alone (MGP (cid:2) / (cid:2) OPN (cid:3) / (cid:3) ) showed calcification of their arteries as early as 2 weeks (wk) after birth (0.33 (cid:4) 0.01 mmol/g dry weight), and the expression of OPN in the calcified arteries was greatly up-regulated compared with MGP wild-types. OPN accumulated adjacent to the mineral and colocalized to surrounding cells in the calcified media. Cells synthesizing OPN lacked smooth muscle (SM) lineage markers, SM (cid:5) -actin and SM22 (cid:5) . However, most of them were not macrophages. Importantly, mice deficient in both MGP and OPN had twice as much arterial calcification as MGP (cid:2) / (cid:2) OPN (cid:3) / (cid:3) at 2 wk, and over 3 times as much at 4 wk, suggesting an inhibitory effect of OPN in vascular calcification. Moreover, these mice died significantly earlier (4.4 (cid:4) 0.2 wk) than MGP (cid:2) / (cid:2) OPN (cid:3) / (cid:3) counterparts (6.6 (cid:4) 1.0 wk). The cause of death in these animals was found to be vascular rupture followed by hemorrhage, most likely due to enhanced calcification. These studies are the first to demonstrate a role for OPN as an inducible inhibitor of ectopic calcification in vivo. region within the aortic wall was measured using direct 3D morphometric analysis Statistical Analysis. Data, means SEM, were analyzed with Student’s t test or analysis of variance to determine the significance of differences. The association of two variables, shown as correlation coefficient, was considered statistically significant at a P value of (cid:11) 0.05. The differences between observed data and expected data were analyzed by (cid:12) 2 test and considered to be statistically significant at a P value of (cid:11) 0.05.
Terahertz particle-in-liquid sensing with spoof surface plasmon polariton waveguides

We present a highly sensitive microfluidic sensing technique for the terahertz (THz) region of the electromagnetic spectrum based on spoof surface plasmon polaritons (SPPs). By integrating a microfluidic channel in a spoof SPP waveguide, we take advantage of these highly confined electromagnetic modes to create a platform for dielectric sensing of liquids. Our design consists of a domino waveguide, that is, a series of periodically arranged rectangular metal blocks on top of a metal surface that supports the propagation of spoof SPPs. Through numerical simulations, we demonstrate that the transmission of spoof SPPs along the waveguide is extremely sensitive to the refractive index of a liquid flowing through a microfluidic channel crossing the waveguide to give an interaction volume on the nanoliter scale. Furthermore, by taking advantage of the insensitivity of the domino waveguide’s fundamental spoof SPP mode to the lateral width of the metal blocks, we design a tapered waveguide able to achieve further.
Clonal B‐cell proliferations developing in the background of primary cutaneous CD4+ small/medium T‐cell lymphoproliferative disorder: A case series

Clonal B‐cell proliferations and B‐cell lymphomas may co‐occur in the background of follicular helper T‐cell (TFH)‐derived lymphomas, most associated with EBV, which has been a well‐known fact for many years in the prototypical entity “TFH lymphoma, angioimmunoblastic‐type. ” Primary cutaneous CD4+ small/medium T‐cell lymphoproliferative disorder (CD4+ PCSM‐LPD) is also a TFH‐derived clonal proliferation. We searched the archives and identified four cases of CD4+ PCSM‐LPD with accompanying clonal B‐cell proliferation (one of which showed EBER positivity), and one longstanding case of CD4+ PCSM‐LPD, in the background of which a B‐cell lymphoma had developed. These five cases broaden experience on CD4+ PCSM‐LPD with accompanying B‐cell proliferations and also support routine evaluation of these cases with EBV in situ hybridization, to better determine whether or not there is an association with EBV.
Multiple B-Value Model-Based Residual Network (MORN) for Accelerated High-Resolution Diffusion-Weighted Imaging

Single-Shot Echo Planar Imaging (SSEPI) based Diffusion Weighted Imaging (DWI) has shortcomings such as low resolution and severe distortions. In contrast, Multi-Shot EPI (MSEPI) provides optimal spatial resolution but increases scan time. This study proposed a Multiple b-value mOdel-based Residual Network (MORN) model to reconstruct multiple b-value high-resolution DWI from undersampled k-space data simultaneously. We incorporated Parallel Imaging (PI) into a residual U-net to reconstruct multiple b-value multi-coil data with the supervision of MUltiplexed Sensitivity-Encoding (MUSE) reconstructed Multi-Shot DWI (MSDWI). Moreover, asymmetric concatenations among different b-values and the combined loss to back propagate helped the feature transfer. After training and validation of the MORN in a dataset of 32 healthy cases, additional assessments were performed on 6 patients with different tumor types. The experimental results demonstrated that the MORN model outperformed conventional PI reconstruction (i.e. SENSE) and two state-of-the-art deep learning methods (SENSE-GAN and VSNet) in terms of PSNR (Peak Signal-to-Noise Ratio), SSIM (Structual SIMilarity) and apparent diffusion coefficient maps. In addition, using the pre-trained model under DWI, the MORN achieved consistent fractional anisotrophy and mean diffusivity reconstructed from multiple diffusion directions. Hence, the proposed method shows potential in clinical application according to the observations on tumor patients as well as images of multiple diffusion directions.
Damage of brown planthopper (BPH) Nilaparvata lugens and rice leaf folder (LF) Cnaphalocrocis medinalis in parent plants lead to distinct resistance in ratoon rice

ABSTRACT Herbivore-induced defense responses are often specific, whereas plants could induce distinct defense responses corresponding to infestation by different herbivorous insects. Brown plant hopper (BPH) Nilaparvata lugens, a phloem-feeding insect, and rice leaf folder (LF) Cnaphalocrocis medinalis, a chewing insect, are both specialist herbivores on rice. To characterize the distinct resistance primed by prior damage to these two specialist herbivores, we challenged rice plants with two herbivores during vegetative growth of parent plants and assessed plant resistance in subsequent ratoons. Here, we show that LF and BPH induce different suites of defense responses in parent rice plants, LF induced higher level of JA accumulation and OsAOS, OsCOI1 transcripts, while BPH induced higher accumulation of SA and OsPAL1 transcripts. Moreover, an apparent loss of LF resistance was observed in OsAOS, OsCOI1 RNAi lines. Ratoon plants generated from parents receiving prior LF infestation exhibited higher jasmonic acid (JA) levels and elevated levels of transcripts of defense-related genes associated with JA signaling, while ratoon generated from parents receiving prior BPH infestation exhibited higher salicylic acid (SA) levels and elevated levels of transcripts of defense-related genes associated with SA signaling. Moreover, previous LF infestation obviously elevated ratoons resistance to LF, while previous infestation by BPH led to enhanced resistance in ratoons to BPH. Pre-priming of ratoons defense to LF was significantly reduced in OsAOS and OsCOI1 RNAi plant, but silencing OsAOS and OsCOI1 did not attenuate ratoons resistance to BPH. These results suggest that infestation of two specialist herbivores with different feeding styles in parent crop led to distinct defense responses in subsequent rations, and the acquired resistance to LF in ratoons is associated with priming of jasmonic acid-dependent defense responses.
DETERMINATION ASSOCIATION OF ANTHROPOMETRIC AND PERFORMANCE ABILITY IN SEPAK TAKRAW YOUTH ATHLETE USING UNSUPERVISED MULTIVARIATE

The purpose of the current study was to determine the most important variable associated with the performance ability. Thirty-eight players of sepak takraw with the mean age 16.21 (0.93) years old participated in this study. A total 12 anthropometric measurements and sepak takraw performance ability were examined. The PCA revealed nine anthropometric parameters as most significant in sepak takraw. The significant parameters were then classified into two group of expertise labeled as high (HPT) and low performance team (LPT) using HACA. DA standard mode exposed hip circumference and performance ability are the best parameter to differentiate between the two groups, whereas both stepwise forward and backward exposed performance ability is significant parameter. The present study identified hip circumference and performance ability as foretelling factors of performance in sepak takraw. Keywords: anthropometric; multivariate analysis; performance ability; sepak takraw; youth athletes
Lactoferrin improves the immune response and resistance of silver carp, a hematological, light (histochemical and immunohistochemical), fluorescent, and scanning electron microscopic study

Lactoferrin is a multifunctional protein that has roles in iron metabolism, cell generation and differentiation, and antibacterial, antiviral, and antiparasitic activity. The study aim was to evaluate the effect of dietary lactoferrin powder on the innate immune response and disease resistance of silver carp (Hypophthalmichthys molitrix) infected with Vibrio vulnificus. The study also investigates immune cells in different organs including gills, intestine, spleen, liver, and pancreas using light, scan electron microcopy. Two diet regimes, a basic diet and a 600‐mg/kg bovine lactoferrin‐supplemented diet were used. The fish were fed for 30 successive days. We included 270 apparently healthy silver carp (H. Molitrix) with an average body weight of 10 ± 2 g/fish. Addition of lactoferrin to the diet led to an increase in the activity of non‐specific immune parameters; lymphocytes, and in the monocytes percentage, and in total protein, serum globulin, and albumin, with a corresponding increase in phagocytic activities and indices. The histological analysis revealed increase immune cells such as lymphocytes in intestine, rodlet cells in liver, gills, and pancreas, and the macrophages in liver, pancreas as well as spleen. The lactoferrin‐supplemented diet significantly increased survival and disease resistance following V. vulnificus challenge, which seemed to correspond to an enhancement of non‐specific immune functions in H. molitrix.
Encountering Overdose: Examining the Contexts and Correlates of US College Students’ Overdose Experiences

Abstract Background As overdose rates increase, it is critical to better understand the causes and contexts of overdose, particularly for college students who exhibit high rates of alcohol and drug use. The purpose of this study was to examine the social contexts of U.S. college students’ overdose experiences (their own, witnessed, and family’/friends’), and to assess the correlates of personal overdose. Methods A cross-sectional survey containing open- and closed-ended questions about overdose encounters was completed by undergraduate students at a southeastern American university (n = 1,236). Descriptive frequencies assessed prevalence, substance involvement, and fatalities associated with different encounter types. A content analysis of open-ended responses examined the social contexts of encounters. Multivariate logistic regression was used to assess the correlates of personal overdose. Results Forty-one percent of respondents reported at least one type of overdose encounter and witnessed overdose was most common. Substances involved varied across encounter type and 20-40% of respondents reported overdose-related fatalities. Students who encountered overdose often reported multiple experiences and many attributed overdoses to mixing substances. Respondents commonly encountered overdose as intervening bystanders and overdose events were often perceived to be intentional or the result of using substances to cope with stress/mental health concerns. Personal overdose was significantly associated with having ever mixed alcohol with prescription drugs, been diagnosed with a mental disorder, witnessed an overdose, and had a family member/friend overdose. Conclusion Findings suggest a need for future research into the contexts and consequences of students’ overdose encounters to more effectively tailor overdose prevention/response initiatives within college communities.
A 10‐mW 3.9‐dB NF transformer‐based V‐band low‐noise amplifier in 65‐nm CMOS

A V‐band low‐noise amplifier (LNA) employing a Gm‐boosting technique is presented in this paper. With the transformers, which are applied between the adjacent stages, the transconductances of the following transistors are boosted. Thus, the gain of the circuit is effectively enhanced. The noise figure (NF) is also decreased with the technique. Utilizing a commercial 65‐nm CMOS technology, the LNA is demonstrated. The measurement results show that the LNA achieves a maximum gain of 17.4 dB at 57.1 GHz with 10.9‐GHz 3‐dB gain bandwidth. The measured NF of the LNA is from 3.95 dB to 4.6 dB at 53 to 64 GHz. The tested input 1‐dB gain compression point (IP1dB) is −14.2 dBm at 57 GHz. The DC power consumption is only 10 mW with 1‐V power supply. The chip area is only 0.255 mm2 with all testing pads.
Capacity building in low- and middle-income countries

Abstract: NPs are an essential resource in the organization and creation of capacity-building efforts to improve healthcare across the globe. There are limited data to guide the creation of effective supplementary education projects. Recent evidence highlights the essential components of sustainability, curriculum development, and program evaluation. NPs are an essential resource in the organization and creation of capacity-building efforts to improve healthcare across the globe. There are limited data to guide the creation of effective supplementary education projects. Recent evidence highlights the essential components of sustainability, curriculum development, and program evaluation.