Miracle-dz/UniFAR-FTUs huggingface dataset:
Cecropin A Alleviates LPS-Induced Oxidative Stress and Apoptosis of Bovine Endometrial Epithelial Cells

Simple Summary Dairy cows receiving a prolonged high-concentrate diet are highly susceptible to sub-acute ruminal acidosis, leading to an elevated concentration of lipopolysaccharides (LPSs) in the peripheral blood circulation. A high concentration of LPS can cause severe oxidative stress, leading to various inflammations, which can cause inflammatory damage and even apoptosis. Antimicrobial peptides (AMPs) not only have antimicrobial effects but also play roles in cells including anti-inflammation, antioxidative stress, and anti-apoptosis. In this study, we found that cecropin A, as an AMP, relieved LPS-induced oxidative stress in dairy cow endometrial epithelial cells and inhibited the inflammatory response caused by oxidative damage. Additionally, we discovered that cecropin A can effectively alleviate LPS-induced cell apoptosis by inhibiting the mitochondrial-dependent apoptotic pathway. Abstract Dairy cows receiving a prolonged high-concentrate diet express an elevated concentration of lipopolysaccharides (LPSs) in the peripheral blood circulation, accompanied by a series of systemic inflammatory responses; however, the specific impacts of inflammation are yet to be determined. Cecropin-like antimicrobial peptides have become a research hotspot regarding antimicrobial peptides because of their excellent anti-inflammatory activities, and cecropin A is a major member of the cecropin family. To elucidate the mechanism of cecropin A as anti-inflammatory under the condition of sub-acute ruminal acidosis (SARA) in dairy cows, we induced inflammation in bEECs with LPS (10 µg/mL) and then added cecropin A (25 µM). Afterwards, we detected three categories of indexes including oxidative stress indices, inflammation-related genes, and apoptosis-related genes in bovine endometrial epithelial cells (bEECs). The results indicated that cecropin A has the ability to reduce inflammatory factors TNF-α, IL-1β, and IL-8 and inhibit the MAPK pathway to alleviate inflammation. In addition, cecropin A is able to reduce reactive oxygen species (ROS) levels and alleviates LPS-induced oxidative stress and mitochondrial dysfunction by downregulating NADPH Oxidase (NOX), and upregulating catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD). Furthermore, cecropin A demonstrates the ability to inhibit apoptosis by suppressing the mitochondrial-dependent apoptotic pathway, specifically Fas/FasL-caspase-8/-3. The observed increase in the Bcl-2/Bax ratio, a known apoptosis regulator, further supports this finding. In conclusion, our study presents novel solutions for addressing inflammatory responses associated with SARA.
State-of-the-Art Review on Inhalable Lipid and Polymer Nanocarriers: Design and Development Perspectives

Nowadays, the interest in research towards the local administration of drugs via the inhalation route is growing as it enables the direct targeting of the lung tissue, at the same time reducing systemic side effects. This is of great significance in the era of nucleic acid therapeutics and personalized medicine for the local treatment of severe lung diseases. However, the success of any inhalation therapy is driven by a delicate interplay of factors, such as the physiochemical profile of the payload, formulation, inhalation device, aerodynamic properties, and interaction with the lung fluids. The development of drug delivery systems tailored to the needs of this administration route is central to its success and to revolutionize the treatment of respiratory diseases. With this review, we aim to provide an up-to-date overview of advances in the development of nanoparticulate carriers for drug delivery to the lung tissue, with special regard concerning lipid and polymer-based nanocarriers (NCs). Starting from the biological barriers that the anatomical structure of the lung imposes, and that need to be overcome, the current strategies to achieve efficient lung delivery and the best support for the success of NCs for inhalation are highlighted.
Silicon in prevention of atherosclerosis and other age-related diseases

Researchers' interest in silicon as an element important for the functioning of the animal and human body began in the 1970s. Soluble compounds of silicon bioavailable from water and food seem to have important meaning for life processes occurring in the body. So far, researchers have focused on the significance of silicon for the development of bones and connective tissue, and its role in preventing neurodegenerative diseases and atherosclerosis. Despite numerous studies, the role of silicon as an active element in the human body is poorly understood. Since the involvement of lipid oxidation and inflammatory processes in the pathogenesis of atherosclerosis is well known, this article summarizes and discusses the current research and scientific observations regarding silicon, primarily in terms of its beneficial influence on redox and anti-inflammatory reactions and the lipid profile. The association of silicon with the stabilization of the structure of glycosaminoglycans and their protein complexes may also support the anti-atherosclerotic effect. The authors attempted to collect and present existing publications that could confirm the beneficial role of dietary silicon in the prevention of age-related diseases and explain the potential mechanisms of its action.
Development of a standard capture efficiency test method for residential kitchen ventilation

Cooking activities are a major source of indoor air pollutants. To control pollutants generated from cooking activities, a range hood is commonly used in residential kitchens. Several building codes require that a range hood be installed in new homes to control pollutants from cooking, and the required airflow rates for range hoods are specified by indoor air quality standards. However, airflow alone does not show how much of the cooking pollutants are exhausted by the range hood. A better metric to evaluate range hood indoor air quality performance is capture efficiency—the fraction of contaminants emitted during cooking that are exhausted directly to the outside via the range hood. The current article summarizes the development of a range hood capture efficiency test method for use in laboratory testing and equipment rating.
Is COVID-19 Infection a Multiorganic Disease? Focus on Extrapulmonary Involvement of SARS-CoV-2

First described in December 2019 in Wuhan (China), COVID-19 disease rapidly spread worldwide, constituting the biggest pandemic in the last 100 years. Even if SARS-CoV-2, the agent responsible for COVID-19, is mainly associated with pulmonary injury, evidence is growing that this virus can affect many organs, including the heart and vascular endothelial cells, and cause haemostasis, CNS, and kidney and gastrointestinal tract abnormalities that can impact in the disease course and prognosis. In fact, COVID-19 may affect almost all the organs. Hence, SARS-CoV-2 is essentially a systemic infection that can present a large number of clinical manifestations, and it is variable in distribution and severity, which means it is potentially life-threatening. The goal of this comprehensive review paper in the series is to give an overview of non-pulmonary involvement in COVID-19, with a special focus on underlying pathophysiological mechanisms and clinical presentation.
Improvement of surface run‐off in the hydrological model ParFlow by a scale‐consistent river parameterization

We propose an improvement of the overland‐flow parameterization in a distributed hydrological model, which uses a constant horizontal grid resolution and employs the kinematic wave approximation for both hillslope and river channel flow. The standard parameterization lacks any channel flow characteristics for rivers, which results in reduced river flow velocities for streams narrower than the horizontal grid resolution. Moreover, the surface areas, through which these wider model rivers may exchange water with the subsurface, are larger than the real river channels potentially leading to unrealistic vertical flows. We propose an approximation of the subscale channel flow by scaling Manning's roughness in the kinematic wave formulation via a relationship between river width and grid cell size, following a simplified version of the Barré de Saint‐Venant equations (Manning–Strickler equations). The too large exchange areas between model rivers and the subsurface are compensated by a grid resolution‐dependent scaling of the infiltration/exfiltration rate across river beds. We test both scaling approaches in the integrated hydrological model ParFlow. An empirical relation is used for estimating the true river width from the mean annual discharge. Our simulations show that the scaling of the roughness coefficient and the hydraulic conductivity effectively corrects overland flow velocities calculated on the coarse grid leading to a better representation of flood waves in the river channels.
Applying the epidemic spreading model on the brain network to explain effective connectivity

Network science studies a complex system as a network to capture the connectivity patterns and topological features. Different network topologies have been observed to shape dynamic spread- ing processes on the network in various ways, while the exact relationship is complicated and not yet fully understood. Epidemic models are often applied to describe the spreading process on a network and to facilitate studying the dynamic interactions. We apply a simple epidemic model, the Susceptible-Infected-Susceptible (SIS) model, on the structural brain network to explore the topological properties that drive the dynamic processes. A recent study examined the transfer entropy of empirical data and observed a dominant posterior-anterior spreading pattern in the brain. In both transfer entropy and delayed correlation measures, we show that hubs are more sending information to the network than lower degree nodes. With our continuous-time simu- lations, we also found the empirically-observed posterior-anterior global pattern. Based on our results, the brain topology of hubs mainly located at the back of the brain seems to be responsible for the emergence of the global pattern.
Melatonin derivative 6a as a PARP-1 inhibitor for the treatment of Parkinson’s disease

Both continuous oxidative stress and poly (ADP-ribose) polymerase 1 (PARP-1) activation occur in neurodegenerative diseases such as Parkinson’s disease. PARP-1 inhibition can reverse mitochondrial damage and has a neuroprotective effect. In a previous study, we synthesized melatonin derivative 6a (MD6a) and reported that it has excellent antioxidant activity and significantly reduces α-synuclein aggregation in Caenorhabditis elegans; however, the underlying mechanism is largely unknown. In the present study, we revealed that MD6a is a potential PARP-1 inhibitor, leading to mammalian targe of rapamycin/heat shock factor 1 signaling downregulation and reducing heat shock protein 4 and 6 expression, thus helping to maintain protein homeostasis and improve mitochondrial function. Together, these findings suggest that MD6a might be a viable candidate for the prevention and treatment of Parkinson’s disease.
Changes in Tight Junction Protein Expression Levels but Not Distribution in Commercial White and Brown Laying Hens Supplemented with Chondrus crispus or Ascophyllum nodosum Seaweed

Simple Summary The intestinal epithelium is the layer of cells that line the digestive tract, and it acts both as a selective filter to facilitate the absorption of nutrients and a protective barrier to toxins and infection. The functional integrity of this constantly renewing cell layer depends on so-called tight junction proteins, a class that includes two regulatory proteins called occludin and zonula occludens-1 (ZO-1). We studied the effect of two different prebiotic seaweed supplements for 41 weeks on occludin and ZO-1 expression in two genetic lines of laying hen. Differences between genetic lines in the level of occludin in a highly absorptive section of the small intestine called the jejunum were found. There also were differences due to seaweed supplementation in the ZO-1 protein, and red seaweed supplementation appeared to have more impact in the brown laying hen strain than in the white strain. Overall, our results are consistent with previous studies showing genetic differences in diet response. Abstract It is proposed that prebiotic diet supplements improve intestinal function, in part by improving the barrier function of the intestinal epithelium with an associated increase in the expression of tight junction proteins, including occludin and zonula occludens-1 (ZO-1). We examined the expression of these proteins in two strains of laying hens (Lohman LSL-lite (White) and Lohman Brown-lite (Brown)) who were supplemented or not with 3% Chondrus crispus or 0.5% Ascophyllum nodosum seaweeds from 31 to 72 weeks of age. Occludin was localized to the lateral surfaces and across the intestinal epithelium in all animals. Reactivity for ZO-1 was concentrated at the apicolateral epithelial cell membrane border. Mood’s median test indicated that White hens may express more occludin in villus epithelium (median intensity 3.5 vs. 2.5 in Brown hens, p = 0.06) but less ZO-1 in the deep cryptal epithelium (median intensity 1.5 vs. 2.5 in Brown hens, p = 0.06). Western blotting also showed higher levels of occludin in White than Brown hens (p < 0.05). A decrease in ZO-1 Western blot expression was associated with Chondrus crispus supplementation in comparison to controls (p < 0.05), but not with Ascophyllum nodosum supplementation (p > 0.05). In conclusion, genetic strain and dietary seaweed supplements affect tight junction regulatory protein expression levels but do not impact the anatomical distribution, as seen in cryosections.
Artificial Radionuclides 137 Cs and 90 Sr in the Components of the Ecosystems of the Salt Lakes of the Crimea

In 2016, for the first time a radioecological study was made of 11 salt lakes of the Crimea to investigate the contamination of their ecosystems by 137Cs and 90Sr. There was a positive correlation between the salt content and the concentration of anthropogenic radionuclides 90Sr and 137Cs in the water of the studied groups of lakes. Absorbed doses of 137Cs and 90Sr radiation in aquatic plants in the lakes were 7.7∙10-6 and 3.2∙10-6 Gy/year, respectively, and lay within the “Uncertainty Zone” according to the scale “Zones of Chronic Exposure to Ionizing Radiation”, proposed by Polikarpov.
Exploring Brain Dynamics via EEG and Steady-State Activation Map Networks in Music Composition

In recent years, the integration of brain–computer interface technology and neural networks in the field of music generation has garnered widespread attention. These studies aimed to extract individual-specific emotional and state information from electroencephalogram (EEG) signals to generate unique musical compositions. While existing research has focused primarily on brain regions associated with emotions, this study extends this research to brain regions related to musical composition. To this end, a novel neural network model incorporating attention mechanisms and steady-state activation mapping (SSAM) was proposed. In this model, the self-attention module enhances task-related information in the current state matrix, while the extended attention module captures the importance of state matrices over different time frames. Additionally, a convolutional neural network layer is used to capture spatial information. Finally, the ECA module integrates the frequency information learned by the model in each of the four frequency bands, mapping these by learning their complementary frequency information into the final attention representation. Evaluations conducted on a dataset specifically constructed for this study revealed that the model surpassed representative models in the emotion recognition field, with recognition rate improvements of 1.47% and 3.83% for two different music states. Analysis of the attention matrix indicates that the left frontal lobe and occipital lobe are the most critical brain regions in distinguishing between ‘recall and creation’ states, while FP1, FPZ, O1, OZ, and O2 are the electrodes most related to this state. In our study of the correlations and significances between these areas and other electrodes, we found that individuals with musical training exhibit more extensive functional connectivity across multiple brain regions. This discovery not only deepens our understanding of how musical training can enhance the brain’s ability to work in coordination but also provides crucial guidance for the advancement of brain–computer music generation technologies, particularly in the selection of key brain areas and electrode configurations. We hope our research can guide the work of EEG-based music generation to create better and more personalized music.
Photometric and colorimetric studies of target objects using small and medium-size telescopes

We report on follow-up studies of transient events and objects, which have been conducted in recent years using ground-based optical observations. BVRI photometry has been performed with small and medium-sized telescopes located in Europe and North America. The purpose of these studies, which are mainly focused on objects of unknown nature, is to reveal features of
Gestion de l'incertitude et de l'ignorance, dans l'évaluation de la probabilité de déclenchement des éboulements rocheux

The general methodology for landslide risk management can be applied to rock falls. The uncertainties which affect the failure hazard characterization are described. They explain that the rupture probability is usually estimated in a qualitative way. New approaches are proposed for a quantitative evaluation of rock fall hazard. For a diffuse hazard, failure frequency and impact frequency downslope can be quantified. The failure probability for localized hazards is difficult to estimate. Coupling the classical qualitative geomechanical approach with the frequency based historical approach allows to verify the plausibility of previsions. A quantitative geomechanical approach can be envisaged in some cases where the internal structure of the rock wall is sufficiently known, but it must be associated with an historical one.
EVALUATION OF THE POTENTIAL HAZARD OF MANUFACTURED METAL-BASED NANOMATERIALS TO HEALTH OF AQUATIC ECOSYSTEMS: STATE OF THE ART

Rapidly growing production and use of engineered nanomaterials (NMs) is accompanied by increased emissions into the environment. Existing data gaps in the scientific knowledge on the fate of NMs in the environment and their potential biological adverse effects makes the evaluation of the environmental risks of NMs difficult if not impossible. We propose that the assessment of environmental risks and regulation and control of the release of metal-based NMs should be performed on the basis of total concentration of metals comprising these NMs. This would assure that the safe levels of NMs in the environment were not exceeded until enough environmentally relevant tests have been carried out for more realistic evaluations.
Dynamic Searchable Symmetric Encryption Schemes Supporting Range Queries with Forward/Backward Privacy

Dynamic searchable symmetric encryption (DSSE) is a useful cryptographic tool in encrypted cloud storage. However, it has been reported that DSSE usually suffers from file-injection attacks and content leak of deleted documents. To mitigate these attacks, forward privacy and backward privacy have been proposed. Nevertheless, the existing forward/backward-private DSSE schemes can only support single keyword queries. To address this problem, in this paper, we propose two DSSE schemes supporting range queries. One is forward-private and supports a large number of documents. The other can achieve backward privacy, while it can only support a limited number of documents. Finally, we also give the security proofs of the proposed DSSE schemes in the random oracle model.
Collective spin dynamics of 
Z2
 vortex crystals in triangular Kitaev-Heisenberg antiferromagnets

We show that the mesoscopic incommensurate Z2 vortex crystals proposed for layered triangular anisotropic magnets can be most saliently identified by two distinctive signatures in dynamical spin response experiments: the presence of pseudo-Goldstone phononlike modes at low frequencies ω, associated with the collective vibrations of the vortex cores, and a characteristic multiscattered intensity profile at higher ω, arising from a large number of Bragg reflections and magnon band gaps. These are direct fingerprints of the large vortex sizes and magnetic unit cells and the solitonic spin profile around the vortex cores.
Diagnostic utility of exome sequencing followed by research reanalysis in human brain malformations

Abstract This study aimed to determine the diagnostic yield of singleton exome sequencing and subsequent research-based trio exome analysis in children with a spectrum of brain malformations seen commonly in clinical practice. We recruited children ≤ 18 years old with a brain malformation diagnosed by magnetic resonance imaging and consistent with an established list of known genetic causes. Patients were ascertained nationally from eight tertiary paediatric centres as part of the Australian Genomics Brain Malformation Flagship. Chromosome microarray was required for all children, and those with pathogenic copy number changes were excluded. Cytomegalovirus polymerase chain reaction on neonatal blood spots was performed on all children with polymicrogyria with positive patients excluded. Singleton exome sequencing was performed through a diagnostic laboratory and analysed using a clinical exome sequencing pipeline. Undiagnosed patients were followed up in a research setting, including reanalysis of the singleton exome data and subsequent trio exome sequencing. A total of 102 children were recruited. Ten malformation subtypes were identified with the commonest being polymicrogyria (36%), pontocerebellar hypoplasia (14%), periventricular nodular heterotopia (11%), tubulinopathy (10%), lissencephaly (10%) and cortical dysplasia (9%). The overall diagnostic yield for the clinical singleton exome sequencing was 36%, which increased to 43% after research follow-up. The main source of increased diagnostic yield was the reanalysis of the singleton exome data to include newly discovered gene–disease associations. One additional diagnosis was made by trio exome sequencing. The highest phenotype-based diagnostic yields were for cobblestone malformation, tubulinopathy and lissencephaly and the lowest for cortical dysplasia and polymicrogyria. Pathogenic variants were identified in 32 genes, with variants in 6/32 genes occurring in more than one patient. The most frequent genetic diagnosis was pathogenic variants in TUBA1A. This study shows that over 40% of patients with common brain malformations have a genetic aetiology identified by exome sequencing. Periodic reanalysis of exome data to include newly identified genes was of greater value in increasing diagnostic yield than the expansion to trio exome. This study highlights the genetic and phenotypic heterogeneity of brain malformations, the importance of a multidisciplinary approach to diagnosis and the large number of patients that remain without a genetic diagnosis despite clinical exome sequencing and research reanalysis.