Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Optimum reproductive performance is essential for well managed and profitable dairy farms (Nebel and Jobst, 1998). Fertility of cow is affected by many specific and nonspecific pathogens of the genital tract. Cervical mucus discharge (CMD) is a A study was carried out on vaginal secretions/aspirates from infertile (acyclic and endometritic) crossbred cows from field and healthy cyclic as well as pregnant crossbred cows of University farm to identify the vaginal microorganisms based on routine cultural examination. The work was carried out on total 36 crossbred cows covering six each regular cyclic (proestrus, estrus, metestrus, diestrus), acyclic, endometritic and 3, 6 and 9 months pregnant animals. The samples of cervico-vaginal mucus/discharge during estrus/ endometritis, and vaginal washings during other phases of estrous cycle as well as pregnancy were collected aseptically using syringe and pipette method. The samples obtained were soon processed for cultural isolation on Blood agar and MacConkey agar, and identified using Gram’s staining and biochemical tests. Bacteria were recovered from all 54 vaginal samples (100%) of cows with different physio-pathological status. During the follicular phase of estrous cycle, the most predominant bacteria isolated were Bacillus Spp. , followed by Corynebacterium Spp. , Staphylococcus Spp. and Streptococcus Spp. , whereas during luteal phase the most predominant bacteria were Staphylococcus Spp. followed by Corynebacterium Spp. , Bacillus Spp. , E. coli and Streptococcus Spp. The most predominant vaginal bacterial isolates during pregnancy in descending order were Staphylococcus Spp. , Bacillus Spp. , Streptococcus Spp. , Klebsiella, E. coli and Pseudomonas. The vaginal aspirates of acyclic cows contained Streptococcus Spp. , Bacillus Spp. and Staphylococcus Spp. , Corynebacterium Spp. , E. coli, and Micrococcus, whereas in endometritis the major isolates were Staphylococcus Spp. , Bacillus Spp. , Streptococcus Spp. , E. coli, Salmonella and Corynebacterium. The findings reflected rich bacterial diversity in the vagina of crossbred cattle with varied physio-pathological conditions. K e y w o r d s
Editorial: Family enterprise: Significant and different

This special issue of Small Enterprise Research (SER) is dedicated to family business, a topic which, although sometimes mentioned in passing in the journal, has not previously been the subject of a special issue. A comparative lack of attention to the special nature of family firms is typical of business scholarship generally where the non-family (or widely-held) corporate form dominates both the business research literature and business education. Family business has also been slow to attract public policy attention, perhaps because family businesses are stereotyped as small, despite the international prominence of huge family-based enterprises such as Newscorp.
PENGARUH PRODUCT BUNDLING TERHADAP PEMBELIAN HANDPHONE MEREK NEXIAN DI KALANGAN MAHASISWA UNIVERSITAS MUHAMMADIYAH PURWOREJO

ABSTRAKTingkat persaingan yang tinggi menyebabkan tingkat inovasi dalam strategi penjualan mengalami peningkatan. Strategi penjualan produk denganmenggunakan bundling semakin banyak digunakan karena memberikan banyakmanfaat bagi kedua belah pihak, produsen dan konsumen. Tujuan dari penelitian ini adalah untuk menganalisis dan memberikanbukti empiris pengaruh produk, harga, promosi, persepsi penghematan, nilaipersepsian, dan kualitas persepsian terhadap perilaku pembelian product bundlinghandphone merek Nexian di kalangan Mahasiswa Universitas MuhammadiyahPurworejo. Populasi dalam penelitian ini adalah Mahasiswa UniversitasMuhammadiyah Purworejo yang membeli product bundling handphone merekNexian, yang kemudian diambil sebagai sampel sebanyak 100 orang denganmenggunakan teknik purposive sampling. Instrumen pengumpulan datamenggunakan kuesioner dengan skala Likert yang sudah diuji cobakan dan telahmemenuhi syarat validitas dan reliabilitas. Metode analisis yang digunakan adalahregresi linear berganda. Berdasarkan hasil perhitungan dengan menggunakan SPSS versi 16diketahui bahwa Produk secara signifikan berpengaruh secara positif terhadapperilaku pembelian konsumen dengan (p value) = 0.019 (< 0.05). Harga secarasignifikan berpengaruh secara positif terhadap perilaku pembelian konsumendengan (p value) = 0.001 (< 0.05). Promosi secara signifikan berpengaruh secarapositif terhadap perilaku pembelian konsumen dengan (p value) = 0.005 (< 0.05). Persepsi penghematan secara signifikan berpengaruh secara positif terhadapperilaku pembelian konsumen dengan (p value) = 0.001 (< 0.05). Nilai persepsiansecara signifikan berpengaruh secara positif terhadap perilaku pembeliankonsumen dengan (p value) = 0.019 (< 0.05). Kualitas persepsian secarasignifikan berpengaruh secara positif terhadap perilaku pembelian konsumendengan (p value) = 0.015 (< 0.05). Secara bersama-sama variabel produk, harga,promosi, persepsi penghematan, nilai persepsian, dan kualitas persepsianberpengaruh terhadap perilaku pembelian konsumen dilihat dari F hitung sebesar25.970 dan signifikansi < 0.05. Sehingga penelitian ini memberikan hasil yangmendukung teori product bundling berpengaruh terhadap perilaku pembeliankonsumen. Kata kunci : bundling, produk, harga, promosi, persepsi penghematan, nilai persepsian, kualitas persepsian, dan perilaku konsumen
Studying the client's role in construction management

Construction industry researchers tend to oversimplify the role of the client in the construction management process. This partly results from the propensity of researchers to use ‘broadcast’ survey method approaches which typically achieve shallow penetration of the client's world. Obtaining access to critical data involves a different relationship between researcher and client. When the client is seen as complex rather than unitary, the history and prehistory of the project loom large. What has occurred in the past can have a crucial effect on the operations of the project team assembled to manage the construction. A pilot study to test the feasibility of obtaining valid information from building clients is described. Twenty ‘points’ or hypotheses about the client's role in construction management are advanced and are to be tested in a forthcoming major study.
Fundamental Tribology Knowledge Serve Modeling and Knowledge Resource Units Construction

Knowledge-intensive is a significant feature of the modern service-oriented industrial structure, and knowledge service is a main part of knowledge science. With research practices and accumulations in the field of tribological knowledge acquisition of turbine, automobile engines and other complex mechanical systems, construction of knowledge base and cooperation with enterprises in chain of “University-Industry-Research”, based on the understanding and experience of conceptual contents and features of tribology, knowledge service and resource unit and other concepts in the process of utilization, this paper studied the expression of modeling knowledge in the process of basic tribological knowledge service, construction of knowledge unit based on knowledge base and the driving force of knowledge acquisition and knowledge flow.
Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33

Galectin-3 (Gal-3) has diverse roles in inflammatory and autoimmune diseases. There is evidence that Gal-3 plays a role in both type 1 and type 2 diabetes. While the role of Gal-3 expression in immune cells invading the pancreatic islets in the experimental model of type 1 diabetes mellitus has been already studied, the importance of the overexpression of Gal-3 in the target β cells is not defined. Therefore, we used multiple low doses of streptozotocin (MLD–STZ)–induced diabetes in C57Bl/6 mice to analyze the effect of transgenic (TG) overexpression of Gal-3 in β cells. Our results demonstrated that the overexpression of Gal-3 protected β cells from apoptosis and attenuated MLD–STZ–induced hyperglycemia, glycosuria, and ketonuria. The cellular analysis of pancreata and draining lymph nodes showed that Gal-3 overexpression significantly decreased the number of pro-inflammatory cells without affecting the presence of T-regulatory cells. As the application of exogenous interleukin 33 (IL-33) given from the beginning of MLD–STZ diabetes induction attenuates the development of disease, by increasing the presence of regulatory FoxP3+ ST2+ cells, we evaluated the potential synergistic effect of the exogenous IL-33 and TG overexpression of Gal-3 in β cells at the later stage of diabetogenesis. The addition of IL-33 potentiated the survival of β cells and attenuated diabetes even when administered later, after the onset of hyperglycemia (12–18 days), suggesting that protection from apoptosis and immunoregulation by IL-33 may attenuate type 1 diabetes.
Microbial Infections Associated with Diabetic Foot Ulcer in Nasiriyah City

Diabetic foot ulcers (DFUs) are a major complication of diabetes lead to development of gangrene associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. This study was aimed to identify the pathogenic microorganisms in diabetic foot ulcers and to test their antibiotic susceptibility. A total of 134 patients with diabetic foot ulcers (DFU) attending to Diabetic center/ Thi-Qar province, South of Iraq were randomly selected. Samples were cultured for isolating bacteria species and Fungi species. Antibiotic sensitivity testing for bacteria species was done by Kirby Bauer disc diffusion method. Elderly diabetic males were frequently observed to develop foot ulcers higher than female. The most of patients 56 (41.79%) were in the age group 51 to 60 years followed by the 36 (26.86%) patients were in age group 41 to 50 years. Out of 162 isolates, bacteria species was 116 (71.6%) more than Fungi species 46 (28.4%). The most isolated bacteria was Proteus mirabilis 25 (15.43%) followed by Enterobacter cloacae 22 (13.58%) and both Escherichia coli and Staphylococcus epidermidis 17 (10.49%). Diabetic foot infections are life-threatening. Many pathogens associated with diabetic foot ulcers.
Dietary Addition With Clostridium butyricum and Xylo-Oligosaccharides Improves Carcass Trait and Meat Quality of Huanjiang Mini-Pigs

This study was conducted to investigate the effects of dietary addition with Clostridium butyricum (CB) and xylo-oligosaccharides (XOS) on growth performance, carcass trait, and meat quality of pigs. A total of 128 Huanjiang mini-pigs with an initial body weight of 9.5 ± 0.1 kg were randomly assigned to one of four groups. The pigs in control (Con) group were fed a basal diet and those in the experimental groups were fed the basal diet supplemented with 0.05% CB (CB group), 0.02% XOS (XOS group), or 0.05% CB + 0.02% XOS (CB + XOS group). Eight replicate pens were used per group with four pigs per pen. On days 28, 56, and 84 of the trial, the growth performance, carcass trait, and meat quality were evaluated. The results showed that dietary CB addition decreased (p < 0.05) the average daily gain and increased (p < 0.05) the ratio of feed intake to body weight gain at day 28 of the trial; CB, XOS, and CB + XOS addition increased (p < 0.05) the backfat thickness at day 84 of the trial compared with the Con group. Dietary CB, XOS, and CB + XOS addition increased (p < 0.05) the pH45min, while decreased (p < 0.05) the marbling score at day 28 of the trial compared with the Con group. Dietary CB + XOS addition increased (p < 0.05) the contents of Ala, Arg, Asp, Gly, His, Leu, Lys, Met, Phe, Ser, Thr, Tyr, and Val in muscle at day 56 of the trial. At day 84 of the trial, dietary CB addition increased the contents of nonessential amino acid (NEAA), total amino acid (TAA), and monounsaturated fatty acid (MUFA), while decreased (p < 0.05) the percentage of C20:1 in muscle compared with the Con group. Collectively, dietary addition with 0.05% CB and 0.02% XOS could not alter the growth performance, but increase carcass trait, meat quality, and muscular nutrient contents in Huanjiang mini-pigs.
Identification of Glutathione Peroxidase Gene Family in Ricinus communis and Functional Characterization of RcGPX4 in Cold Tolerance

Glutathione peroxidases (GPXs) protect cells against damage caused by reactive oxygen species (ROS) and play key roles in regulating many biological processes. Here, five GPXs were identified in the Ricinus communis genome. Phylogenetic analysis displayed that the GPXs were categorized into five groups. Conserved domain and gene structure analyses showed that the GPXs from different plant species harbored four highly similar motifs and conserved exon-intron arrangement patterns, indicating that their structure and function may have been conserved during evolution. Several abiotic stresses and hormone-responsive cis-acting elements existed in the promoters of the RcGPXs. The expression profiles indicated that the RcGPXs varied substantially, and some RcGPXs were coordinately regulated under abiotic stresses. Overexpression of RcGPX4 in Arabidopsis enhanced cold tolerance at seed germination but reduced freezing tolerance at seedlings. The expression of abscisic acid (ABA) signaling genes (AtABI4 and AtABI5), ABA catabolism genes (AtCYP707A1 and AtCYP707A2), gibberellin acid (GA) catabolism gene (AtGA2ox7), and cytokinin (CTK)-inducible gene (AtARR6) was regulated in the seeds of transgenic lines under cold stress. Overexpression of RcGPX4 can disturb the hydrogen peroxide (H2O2) homeostasis through the modulation of some antioxidant enzymes and compounds involved in the GSH-ascorbate cycle in transgenic plants. Additionally, RcGPX4 depended on the MAPK3-ICE1-C-repeat-binding factor (CBF)-COR signal transduction pathway and ABA-dependent pathway to negatively regulate the freezing tolerance of transgenic plants. This study provides valuable information for understanding the potential function of RcGPXs in regulating the abiotic stress responses of castor beans.
Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells via Facilitating the Expression and Secretion of ENPP2

Background: Hepatitis B virus (HBV) infection is a major risk factor causing hepatocellular carcinoma (HCC) development, but the molecular mechanisms are not fully elucidated. It has been reported that virus infection induces ectonucleotide pyrophosphatase-phosphodiesterase 2 (ENPP2) expression, the latter participates in tumor progression. Therefore, the aim of the present study was to investigate whether HBV induced HCC malignancy via ENPP2. Methods: HCC patient clinical data were collected and prognosis was analyzed. Transient transfection and stable ectopic expression of the HBV genome were established in hepatoma cell lines. Immunohistochemical staining, RT-qPCR, western blot, and ELISA assays were used to detect the expression and secretion of ENPP2. Finally, CCK-8, colony formation, and migration assays as well as a subcutaneous xenograft mouse model were used to investigate the influence of HBV infection, ENPP2 expression, and activated hepatic stellate cells (aHSCs) on HCC progression in vitro and in vivo. Results: The data from cancer databases indicated that the level of ENPP2 was significant higher in HCC compared within normal liver tissues. Clinical relevance analysis using 158 HCC patients displayed that ENPP2 expression was positively correlated with poor overall survival and disease-free survival. Statistical analysis revealed that compared to HBV-negative HCC tissues, HBV-positive tissues expressed a higher level of ENPP2. In vitro, HBV upregulated ENPP2 expression and secretion in hepatoma cells and promoted hepatoma cell proliferation, colony formation, and migration via enhancement of ENPP2; downregulation of ENPP2 expression or inhibition of its function suppressed HCC progression. In addition, aHSCs strengthened hepatoma cell proliferation, migration in vitro, and promoted tumorigenesis synergistically with HBV in vivo; a loss-function assay further verified that ENPP2 is essential for HBV/aHSC-induced HCC progression. Conclusion: HBV enhanced the expression and secretion of ENPP2 in hepatoma cells, combined with aHSCs to promote HCC progression via ENPP2.
A Novel Combined Conjugate Therapeutic Cancer Vaccine, Recombinant EGF-CRM197, in Patients With Advanced Solid Tumors: A Phase I Clinical Study

Introduction The therapeutic cancer vaccine recombinant Epidermal Growth Factor (EGF)-CRM197 is a novel combined conjugate EGF with CRM197 as a carrier protein. Immunization with the EGF-CRM197 vaccine can induce high levels of neutralizing anti-EGF antibodies that inhibit EGF/EGFR signaling and thereby suppress growth of tumors that rely on this signaling pathway. Herein, we characterize the humoral immune responses elicited by the recombinant EGF-CRM197 vaccine in patients with advanced solid tumors in a phase I clinical trial and assess the safety, tolerability, and immunogenicity of this vaccine (CTR20190473). Methods A total of 16 subjects were enrolled in this study. Under 6 + 3 design, patients in each dosing cohort were administrated subcutaneously at a dosage of 0.4 mg, 0.8 mg, and 1.6 mg, respectively. The patients received vaccinations for immune induction (once a week for 4 consecutive weeks) and booster vaccinations (once every 4 weeks). Safety evaluation was performed 1 week after the immune induction. Booster vaccination was given until the occurrence of disease progression, intolerance, withdrawal of informed consent by the patient, or negative result of anti-EGF test after two booster vaccinations. Results Vaccination with EGF-CRM197 is safe and well-tolerated in patients with advanced solid tumors. Adverse reactions at the injection site were the most common adverse events (AEs) in recipients. No severe adverse reactions post vaccination were observed in the present study. Vaccinated patients developed a robust neutralizing antibody response triggered by EGF-CRM197 that significantly reduced the levels of EGF in serum. For lung cancer patients who were super good antibody responders (sGAR) to EGF-CRM197, the median progress-free survival (PFS) was 4.83 months, significantly longer than that of the good antibody responder (GAR) patients with lung cancer whose median PFS was 2.10 months (P=0.0018). The median overall survival (OS) of GAR lung cancer patients was 10.67 months while the OS) for sGAR lung cancer patients was not reached until analysis was performed. The median follow-up of the sGAR lung cancer patients was 14.6 months. Conclusion Our study demonstrates that the recombinant EGF-CRM197 therapeutic cancer vaccine can induce a good immune response in patients with advanced solid tumors and is safe and well tolerated, which ensures further clinical development of the vaccine for extending the survival time of EGF-CRM197 sensitive patients with advanced solid tumors. Clinical Trial Registration http://www.chinadrugtrials. org.cn, identifier CTR20190473, EGF-CRM197.
Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck

Ducks are the natural host and reservoir of influenza A virus (IAV), and as such are permissive to viral replication while being unharmed by most strains. It is not known which mechanisms of viral control are globally regulated during infection, and which are specific to tissues during infection. Here we compare transcript expression from tissues from Pekin ducks infected with a recombinant H5N1 strain A/Vietnam 1203/04 (VN1203) or an H5N2 strain A/British Columbia 500/05 using RNA-sequencing analysis and aligning reads to the NCBI assembly ZJU1.0 of the domestic duck (Anas platyrhynchos) genome. Highly pathogenic VN1203 replicated in lungs and showed systemic dissemination, while BC500, like most low pathogenic strains, replicated in the intestines. VN1203 infection induced robust differential expression of genes all three days post infection, while BC500 induced the greatest number of differentially expressed genes on day 2 post infection. While there were many genes globally upregulated in response to either VN1203 or BC500, tissue specific gene expression differences were observed. Lungs of ducks infected with VN1203 and intestines of birds infected with BC500, tissues important in influenza replication, showed highest upregulation of pattern recognition receptors and interferon stimulated genes early in the response. These tissues also appear to have specific downregulation of inflammatory components, with downregulation of distinct sets of proinflammatory cytokines in lung, and downregulation of key components of leukocyte recruitment and complement pathways in intestine. Our results suggest that global and tissue specific regulation patterns help the duck control viral replication as well as limit some inflammatory responses in tissues involved in replication to avoid damage.
The Pathogenesis of COVID-19 Myocardial Injury: An Immunohistochemical Study of Postmortem Biopsies

Rationale Myocardial injury associates significantly and independently with mortality in COVID-19 patients. However, the pathogenesis of myocardial injury in COVID-19 remains unclear, and cardiac involvement by SARS-CoV-2 presents a major challenge worldwide. Objective This histological and immunohistochemical study sought to clarify the pathogenesis and propose a mechanism with pathways involved in COVID-19 myocardial injury. Methods and Results Postmortem minimally invasive autopsies were performed in six patients who died from COVID-19, and the myocardium samples were compared to a control group (n=11). Histological analysis was performed using hematoxylin-eosin and toluidine blue staining. Immunohistochemical (IHC) staining was performed using monoclonal antibodies against targets: caspase-1, caspase-9, gasdermin-d, ICAM-1, IL-1β, IL-4, IL-6, CD163, TNF-α, TGF-β, MMP-9, type 1 and type 3 collagen. The samples were also assessed for apoptotic cells by TUNEL. Histological analysis showed severe pericardiocyte interstitial edema and higher mast cells counts per high-power field in all COVID-19 myocardium samples. The IHC analysis showed increased expression of caspase-1, ICAM-1, IL-1β, IL-6, MMP-9, TNF-α, and other markers in the hearts of COVID-19 patients. Expression of caspase-9 did not differ from the controls, while gasdermin-d expression was less. The TUNEL assay was positive in all the COVID-19 samples supporting endothelial apoptosis. Conclusions The pathogenesis of COVID-19 myocardial injury does not seem to relate to primary myocardiocyte involvement but to local inflammation with associated interstitial edema. We found heightened TGF-β and interstitial collagen expression in COVID-affected hearts, a potential harbinger of chronic myocardial fibrosis. These results suggest a need for continued clinical surveillance of patients for myocardial dysfunction and arrythmias after recovery from the acute phase of COVID-19.