Miracle-dz/UniFAR-FTUs huggingface dataset:
Altered Ca 2+ signaling in skeletal muscle fibers of the R6/2 mouse, a model of Huntington’s disease

excitation–contraction FDB, flexor digitorum brevis; Huntington’s disease; htt, huntingtin; mhtt, mutant htt; MyHC, myosin heavy MyLC, myosin light TTX, tetrodotoxin. Huntington’s disease (HD) is caused by an expanded CAG trinucleotide repeat within the gene encoding the protein huntingtin. The resulting elongated glutamine (poly-Q) sequence of mutant huntingtin (mhtt) affects both central neurons and skeletal muscle. Recent reports suggest that ryanodine receptor–based Ca 2+ signaling, which is crucial for skeletal muscle excitation–contraction coupling (ECC), is changed by mhtt in HD neurons. Consequently, we searched for alterations of ECC in muscle fibers of the R6/2 mouse, a mouse model of HD. We performed fluorometric recordings of action potentials (APs) and cellular Ca 2+ transients on intact isolated toe muscle fibers (musculi interossei), and measured L-type Ca 2+ inward currents on internally dialyzed fibers under voltage-clamp conditions. Both APs and AP-triggered Ca 2+ transients showed slower kinetics in R6/2 fibers than in fibers from wild-type mice. Ca 2+ removal from the myoplasm and Ca 2+ release flux from the sarcoplasmic reticulum were characterized using a Ca 2+ binding and transport model, which indicated a significant reduction in slow Ca 2+ removal activity and Ca 2+ release flux both after APs and under voltage-clamp conditions. In addition, the voltage-clamp experiments showed a highly significant decrease in L-type Ca 2+ channel conductance. These results indicate profound changes of Ca 2+ turnover in skeletal muscle of R6/2 mice and suggest that these changes may be associated with muscle pathology in HD. by cubic spline interpolation. Averaged and smoothed signals were baseline corrected and normalized to the peak of the signal. AP recordings were used for kinetic analysis. A comparison of absolute amplitudes could not be performed because the basal background fluorescence showed considerable fiber-to-fiber vari-ation (probably mainly resulting from dye in the Matrigel coating used for plating the cells). Ca 2+ signaling in skeletal muscle of the mouse HD model Ca 2+ signaling in skeletal muscle can be disturbed by alterations in (a) Ca 2+ influx through plasma membrane channels, (b) release of Ca 2+ from intracellular stores, (c) the membrane potential signal that controls these fluxes, and (d) Ca 2+ removal from the cytoplasm. In this study, we demonstrated changes in all four aspects of muscular Ca 2+ signaling. of Ca 2+ via initiate contraction by binding to C. indicator transport
A fundamental relation between supermassive black holes and dark matter haloes.

1Ghent University, Krijgslaan 281 S9, B-9000 Ghent, Belgium 2Herzberg Institute of Astrophysics, National Researh Council of Canada, 5071 West Saanich Road, RR. 5, Victoria, British Columbia, V9E 2E7, Canada 3SISSA/ISAS Institute, Via Beirut 434014, Trieste, Italy 4European Southern Observatory, Alonso de Cordova 3107, Vitacura, Casilla 19001, Santiago 19, Chile 5Bonn University, Auf dem Hügel 71D, 53121 Bonn, Germany
The effect of progesterone on motility and the acrosome reaction in vitrified and cryopreserved human spermatozoa

English Progesterone has been found to play an important role in the migration of sperm through the reproductive tract and the sperm-oocyte interaction. As well as inducing the acrosome reaction, it has also been shown to induce hyperactive motility and increase sperm velocity. In this blind study we set out to examine whether progesterone could induce beneficial effects in vitrified and slow-frozen spermatozoa, thereby improving their postthaw fertilising ability. For this we divided sperm from 50 ejaculates into three treatment groups; non-cryopreserved, slow-freezing and vitrification. After thawing and a subsequent 2h incubation period to induce capacitation, sperm from each treatment group were treated with 50nM, 25nM progesterone and a control solution for 30 minutes. Thereafter, the sperm suspensions were examined manually and using CASA to assess the proportion of viable and motile sperm, as well as velocity parameters. A chlortetracyclin flourescence assay was used to determine uncapacitated (F), capacitated (B), acrosome reacted (AR) and acrosome reacting (Z) spermatozoa. We did not find any effect of progesterone on the acrosome reaction in the three cryopreservation groups. The results did reveal a higher proportion of progressively motile sperm in vitrified teratozoospermic samples, and improved velocity parameters in slow-frozen normozoospermic and teratozoospermic samples following treatment with 50nM progesterone. We concluded that the progesterone treatment after cryopreservation was able to significantly improve the motility parameters of sperm samples and it had an especially large impact on samples classed as teratozoospermic. These findings are an important step in determining ways of improving the currently low rates of motility in cryopreserved spermatozoa.
Long-term mortality rates and spatial patterns in an old-growth Pinus resinosa forest

Understanding natural mortality patterns and processes of forest tree species is increasingly important given projected changes inmortality owing to global change.With this need inmind, the rate and spatial pattern ofmortalitywas assessed over an 89-year period in a natural-origin Pinus resinosa (Aiton)-dominated system to assess these processes through advanced stages of stand development (stand age 120–209 years). Average annual mortality rates fluctuated through time, yet were within the range reported in other studies (0.60%–3.88% depending on species and sampling interval). Tree mortality was attributed to multiple agents, including the senescence of the short-lived Pinus banksiana Lamb. , windthrow, root-rot fungi (Armillaria ostoyae (Romagn.) Herink), and perhaps infrequent droughts. Despite the often contagious nature of many disturbance agents, the overall spatial pattern of mortality events (the arrangement of dead trees within the fixed initial population of live trees) was random at all scales tested. Similarly, the current spatial pattern of dead trees was predominantly random, despite clustering at small scales (2–4m). These findings underscore the importance of studyingmortality rates, agents, and spatial patterns over long time periods to avoid misinterpreting stochastic mortality events, and their influence on longer term stand structure and
Web Content Classification with Topic and Sentiment Analysis

Automatic classification of web content has been studied extensively, using different learning methods and tools, investigating different datasets to serve different purposes. Most of the studies have made use of content and structural features of web pages. In this study we present a new approach for automatically classifying web pages into pre-defined topic categories. We apply text summarization and sentiment analysis techniques to extract topic and sentiment indicators of web pages. We then build classifiers based on the extracted topic and sentiment features. Our results offer valuable insights and inputs to the development of web detection systems.
Spatial differences and chronology of metasomatism in depleted mantle xenoliths beneath Kimberley, South Africa

Dunite, wehrlite and websterite xenoliths are rare members of the mantle xenolith suite within the Kimberley kimberlites of Southern Africa. They uniquely document a chronological sequence of metasomatism within the strongly depleted Kaapvaal cratonic upper mantle. This metasomtic sequence differs between xenoliths that represent lithologies located close to and xenoliths that come from areas located away from paths of melt infiltration.
OVERVIEW OF ETIOLOGY AND CLINICAL MANAGEMENT OF TINNITUS

Tinnitus is a common medical symptom that can be exhausting. Hearing loss, head injury, ototoxic medication, and depression are risk factors. In this review we discuss the main points of management, etiology and background information of tinnitus. We conducted a narrative review to identify studies with tinnitus etiology and treatment strategies among which were published up to June, 2019. Tinnitus is a perception of sound in proximity to the head in the absence of an outside source. The sound might be a buzzing, sounding, or hissing, although it can likewise seem like other noises. Tinnitus is most typically connected with hearing loss, though it may be an offering signs and symptom of vascular or neurological irregularities. Once a serious underlying medical problem has been ruled out, treatment must be routed at the symptom itself, which badly hinders the quality of life in about 10 percent of patients with tinnitus. Corresponding author: Ohoud Faisal Aburuzaiza, Please cite this article in press Ohoud Faisal Aburuzaiza et al., Overview Of Etiology And Clinical Management Of Tinnitus. , Indo Am. J. P. Sci, 2019; 06[08]. QR code IAJPS 2019, 06 [08], 14390-14395 Ohoud Faisal Aburuzaiza et al ISSN 2349-7750 w w w . i a j p s . c o m Page 1439
Role of employee extraversion personality on job performance: A case study of financial companies in China

This paper describes employees’ personality traits which is one of the challenges that company may confront with when managing human resource as it impact on employees’ job satisfaction and their job performance. There are two main objectives of this study. One is investigating influence of job satisfaction on employee job performance, another one is study the impact of employee personality such as extraversion on job performance. This research applied the theoretical framework based on expectancy and discrepancy of job satisfaction might influence job performance. The research was distributed online to 300 employees of three small and mediumsize financial companies in China, 249 employees responded in order to identify the divergences of reaction between the elements. The result shows that Job Performance will be affected on different Gender, Age, Education, Job Position, income, Extraversion and Job Satisfaction. What’s more, there is a deeply relationship between Job Satisfaction & Job Performance, Extraversion Personality & Job Satisfaction and Extraversion Personality & Job
Platelet disaggregation

Time remains the most daunting obstacle in improving linical outcomes in the treatment of acute ST-segment eleation myocardial infarction (STEMI). Since the landmark ngiographic trial of DeWood et al. (2) demonstrating that TEMI is caused by thrombotic occlusion of the infarctelated artery, pharmacologic and mechanical reperfusion stratgies have been focused on salvaging ischemic but viable yocardium and, thereby, preserving left ventricular function. ibrinolytic therapy saves 39 lives per 1,000 patients treated in he first hour, compared with fewer than 7 lives saved per 1,000 atients treated 13 to 24 h after the onset of chest pain (3). ime-dependent efficacy is also observed for primary percutaeous coronary intervention (PCI) for STEMI, with mortality ncreasing by 61% as door-to-balloon time advances from 0 to 0 min to 180 min (4).
Aalborg Universitet Making a meaningful contribution to theory

Purpose – The need to make a “theoretical contribution” is a presumed mandate that permeates any researcher’s career in the Social Sciences, yet all too often this remains a source of confusion and frustration. The purpose of this paper is to reflect on, and further develops, the principal themes discussed in the “OM Theory” workshop in Dublin in 2011 and the special sessions at the 2011 and the 2013 EurOMA Conferences in Cambridge and Dublin. Design/methodology/approach – This paper presents six short essays that explore the role and use of theory in management research, and specifically ask what is a good or meaningful contribution to theory. The authors comment on the current state of theory in OperationsManagement (OM) (Harry Boer), the type of theories the authors have in OM (Chris Voss), the role of theory in increasing the general understanding of OM problems (Roger Schmenner), whether the authors can borrow theories from other fields or actually have theory “of our own” (Matthias Holweg), the different ways in which a contribution to theory can be made (Martin Kilduff), and how to construct a theoretical argument (Mark Pagell). Findings – The authors argue that theory is fundamental to OM research, but that it is not the inevitable starting point; discovery and observation are equally important and often neglected avenues to contributing to theory. Also, there is no one right way to making a contribution, yet consistency between ontology, epistemology, and claimed contribution is what matters. The authors further argue that the choice of theory is critical, as a common mistake is trying to contribute to high-level theories borrowed from other fields. Finally, the authors recommend using theory parsimoniously, yet with confidence. Originality/value – The paper presents a collection of viewpoints of senior scholars on the need for, and use of, theory in OM research.
Revisiting Clustered Microarchitecture for Future Superscalar Cores

During the past 10 years, the clock frequency of high-end superscalar processors has not increased. Performance keeps growing mainly by integrating more cores on the same chip and by introducing new instruction set extensions. However, this benefits only some applications and requires rewriting and/or recompiling these applications. A more general way to accelerate applications is to increase the IPC, the number of instructions executed per cycle. Although the focus of academic microarchitecture research moved away from IPC techniques, the IPC of commercial processors was continuously improved during these years. We argue that some of the benefits of technology scaling should be used to raise the IPC of future superscalar cores further. Starting from microarchitecture parameters similar to recent commercial high-end cores, we show that an effective way to increase the IPC is to allow the out-of-order engine to issue more micro-ops per cycle. But this must be done without impacting the clock cycle. We propose combining two techniques: clustering and register write specialization. Past research on clustered microarchitectures focused on narrow issue clusters, as the emphasis at that time was on allowing high clock frequencies. Instead, in this study, we consider wide issue clusters, with the goal of increasing the IPC under a constant clock frequency. We show that on a wide issue dual cluster, a very simple steering policy that sends 64 consecutive instructions to the same cluster, the next 64 instructions to the other cluster, and so forth, permits tolerating an intercluster delay of three cycles. We also propose a method for decreasing the energy cost of sending results from one cluster to the other cluster.
Structural Bioinformatics – cum-Immunoinformatic Approaches on the Major Glycoproteins to screen Potential Antigenic Determinants of Varicella Zoster virus in Vaccine design

: The Varivax vaccine presently used against chickenpox is live attenuated, and induces both cell-mediated and humoral immune response whose relative contributions and duration of protection is limited. The physio-chemical properties, prediction, validation of 3D structure, pairwise protein alignment and biological functions of five glycoproteins: gE, gB, gH, gI and gC, and their putative epitopes for potential T cell of HLA I and II have been predicted, using immunoinformatics and structural Bioinformatics tools to identify the effective peptide . gB protein was found to have highly similar structure based on its TM-score. The peptide showing the maximum binding with HLA class 1 molecule is VLLCLVIFL (gE) while LVIFLICTA (gE) and MVIVIVISV (gI) are peptides with HLA class 2 molecules. Hence considering this fact, even though gB had obtained the best TM-score it cannot be considered for proposing an effective subunit vaccine as it has very less allele predicted for both HLA class I and class II. The next best TM-score and hence the maximum alleles shown by gE for HLA class I and gI for HLA class II molecules could be considered in proposing an effective subunit vaccine.
Recovering the degeneracy in quasi two-dimensional artificial colloidal ice

Artificial Spin Ice systems have been used to investigate exotic behaviours of frustrated systems. In this project, I study geometric frustration through computer simulations of Artificial Colloidal Ice which is made of an ensemble of magnetized colloids confined in bistable traps and arranged to form a square lattice. By implementing an offset height between perpendicular traps, it is possible to recover a degenerate ground state in the system.
Origin of the Summertime Synoptic-Scale Wave Train in the Western North Pacific*

The origin of the summertime synoptic wave train in the western North Pacific is investigated with a multilevel, nonlinear baroclinic model. A realistic three-dimensional summer mean state is specified and eigenvectors are calculated by introducing small perturbation initially to the model. Numerical experiments indicate that the origin of the synoptic wave train may arise from instability of the summer mean flow in the presence of a convection–frictional convergence (CFC) feedback. In the lack of the CFC feedback, the summer mean flow supports only a least damped mode, characterized by a northwest–southeast-oriented wave train pattern with a zonal wavelength of 2500 km. In the presence of both the realistic summer mean flow and the CFC feedback, the model reproduces a fast growing mode, whose structure and propagation characters are similar to the observed. Sensitivity experiments with different initial perturbation patterns indicate that the model solution is not sensitive to initial conditions. Further sensitivity experiments reveal that the basic-state vertical shear may affect the growth rate and propagation character of the wave train. An easterly shear may lead to a faster growth and northwestward phase propagation, whereas a westerly shear may favor a slower growth and southeastward phase propagation.
Evaluation of Injection Strategies in Supersonic Nozzle Flow

The ability to manipulate shock patterns in a supersonic nozzle flow with fluidic injection is investigated numerically using Large Eddy Simulations. Various injector configurations in the proximity of the nozzle throat are screened for numerous injection pressures. We demonstrate that fluidic injection can split the original, single shock pattern into two weaker shock patterns. For intermediate injection pressures, a permanent shock structure in the exhaust can be avoided. The nozzle flow can be manipulated beneficially to increase thrust or match the static pressure at the discharge. The shock pattern evolution of injected stream is described over various pressure ratios. We find that the penetration depth into the supersonic crossflow is deeper with subsonic injection. The tight arrangement of the injectors can provoke additional counter-rotating vortex pairs in between the injection.
EEG and Neurocognitive Changes in Chronic Methamphetamine Abuse

Methamphetamine is a potent and highly addictive psychostimulant that has become very popular on the illicit drug market. There is a large amount of literature regarding the neurocognitive changes associated with chronic methamphetamine exposure that are present in currently abstinent individuals. However, the evidence about the possible reversibility of such changes is less clear and only sparse data exist concerning the electroencephalographic (EEG) changes in methamphetamine users. We present a case of a 38 year old male with a 10 year history of continuous abuse of high doses of methamphetamine. The patient has been incapable of adequate social functioning for the last two years and during that period has also been repeatedly admitted to a psychiatric ward due to methamphetamine associated aggressive behavior. After 25 days of abstinence, neurocognitive testing revealed mild cognitive impairment that was most pronounced in psychomotor speed and sequencing in working memory. EEG assessment performed by both visual inspection and quantitative EEG brain mapping showed a slowing of the frequency of dominant occipital activity to the sub alpha frequency band as well as an increase of theta power. No pathological findings were seen on the Magnetic resonance imaging of the brain, however, repeated EEG 20 days later confirmed an abnormal slowing of the dominant alpha frequency. Our findings are in accordance with the limited data available in the literature. However, further studies are needed to confirm the utility of EEG as a marker of methamphetamine toxicity, as well as the association between EEG findings and the neurocognitive performance and the reversibility of both variables in the case of sustained abstinence.
ffects of Statin Treatments on Coronary Plaques ssessed by Volumetric Virtual Histology ntravascular Ultrasound Analysis

esults In overall patients, necrotic core volume significantly reduced (15.7 to 13.7 mm, p .010) and fibrofatty plaque volume increased (4.3 to 5.5 mm, p 0.006) after statin treatments for year. There were no significant differences of changes in either plaque component volume beween simvastatinand rosuvastatin-treated subgroups. In serial comparisons during 1-year followp, simvastatin treatment did not achieve statistically significant changes in fibrofatty plaque (4.1 to .1 mm, p 0.131) and necrotic core volume (15.8 to 14.4 mm, p 0.216). However, there was a ignificant decrease in necrotic core volume (15.5 to 13.0 mm, p 0.015) and an increase in fibroatty plaque volume (4.5 to 5.9 mm, p 0.017) in the rosuvastatin-treated subgroup.
A predictive model for canine dilated cardiomyopathy: a meta-analysis of Doberman Pinscher data. PeerJ,

Dilated cardiomyopathy is a prevalent and often fatal disease in humans and dogs. Indeed dilated cardiomyopathy is the third most common form of cardiac disease in humans, reported to a ff ect approximately 36 individuals per 100,000 individuals. In dogs, dilated cardiomyopathy is the second most common cardiac disease and is most prevalent in the Irish Wolfhound, Doberman Pinscher and Newfoundland breeds. Dilated cardiomyopathy is characterised by ventricular chamber enlargement and systolic dysfunction which often leads to congestive heart failure. Although multiple human loci have been implicated in the pathogenesis of dilated cardiomyopathy, the identified variants are typically associated with rare monogenic forms of dilated cardiomyopathy. The potential for multigenic interactions contributing to human dilated cardiomyopathy remains poorly understood. Consistent with this, several known human dilated cardiomyopathy loci have been excluded as common causes of canine dilated cardiomyopathy, although canine dilated cardiomyopathy resembles the human disease functionally. This suggests additional genetic factors contribute to the dilated cardiomyopathy phenotype.This study represents a meta-analysis of available canine dilated cardiomyopathy genetic datasets with the goal of determining potential multigenic interactions relating the sex chromosome genotype (XX vs. XY) with known dilated cardiomyopathy associated loci on chromosome 5 and the PDK4 gene in the incidence and progression of dilated cardiomyopathy. The results show an interaction between known canine dilated cardiomyopathy loci and an unknown X-linked locus. Our study is the first to test a multigenic contribution to dilated cardiomyopathy and suggest a genetic basis for the known sex-disparity in dilated cardiomyopathy outcomes. heterozygotes (Xx) or susceptible x homozygotes (xx). Unsusceptible X males (XY) are phenotypically identical to unsusceptible X homozygotes (XX) with these individuals requiring at least one DCM susceptibility allele at both of the other loci to develop DCM. All individuals that possess a susceptible X (xY and xx individuals) develop DCM in this model while heterozygotes (Xx) only require a single DCM susceptibility allele at one of the other loci to develop DCM.