Miracle-dz/UniFAR-FTUs huggingface dataset:
MODIFIKASI METODE ISOLASI DNA CETYL TRIMETHYLAMMONIUM BROMIDE (CTAB) UNTUK SAMPEL EPITEL PIPI MANUSIA

Isolation of deoxyribonucleotide (DNA) is an important step in molecular analysis. In this process, DNA must be obtained in sufficient quantities and in good quality for any further analysis. The Cetyl Trimethylammonium Bromide (CTAB) method is commonly used in DNA isolation of plant or fungal. This method is an alternative in DNA isolation since it is easy and inexpensive. This study aims to modify the CTAB method for DNA isolation from human cheek epithelium for any molecular analysis. Epithelial cells were taken from the oral cavity of the researcher. The isolation protocol included cell lysis step with CTAB buffer and proteinase-K, purification step with the addition of chloroform:isoamylalcohol (24:1), precipitation step with isopropanol. The results of the ratio analysis of DNA spectrophotometer at wavelengths of 260 and 280 nm in the range of 1.73-1.85. The quality of DNA isolation was observed by agarose gel electrophoresis and a firm band was obtained after Ethidium Bromide staining. The DNA concentration in both methods ranged from 400-480 mg/mL. The time required for both methods ranges from 2.5-3 hours. The modified CTAB method DNA isolation protocol produces DNA that has good quality and quantity for molecular analysis processes, such as Polymerase Chain Reaction (PCR).
Lemah Abang Railway Crew Payroll Application Program at PT. X

In today's modern era the world of technology and information is growing so rapidly, computers have become a very basic need and able to help all oprasional activities in all fields, therefore it takes a creativity to facilitate and ease daily work. This research aims to facilitate the process of collecting the salaries of THB ka crew in accordance with its service, to help reduce errors in the payment of official money to the ka crew in charge, in order to improve the performance of the Ka Crew in accordance with applicable official regulations, make an ease in the unit of Crew KA THB in the process of data processing, as well as as a positive input in determining a policy, producing programs that support the work of related units. The method used in this research is data collection method such as interview, observation and literature study. The results of research on the Lemah Abang Railway Crew Payroll Application program at PT. The Desktop-Based Indonesian Railway (Persero) is expected to report and pay more easily, precisely, and efficiently.
Narcissism Today: What We Know and What We Need to Learn

Narcissism is of great interest to behavioral scientists and the lay public. Research across the past 20 years has led to substantial progress in the conceptualization, measurement, and study of narcissism. This article reviews the current state of the field, identifying recent advances and outlining future directions. Advances include hierarchical conceptualizations of narcissism across one-factor (narcissism), two-factor (grandiose vs. vulnerable narcissism), and three-factor (agentic extraversion, antagonism, narcissistic neuroticism) levels; the development of measures to assess the components of narcissism; clarification of the relations between narcissism and self-esteem; an understanding of the behavioral and motivational dynamics underlying narcissistic actions and social outcomes; and insight regarding potential fluctuations between narcissistic states. Future directions point in general to increased research using the lower levels of the narcissism hierarchy, especially the three-factor level. At this level, more research on the etiology, heritability, stability, and centrality of the three components is required.
FDA-Approved Excipient N,N-Dimethylacetamide Attenuates In-Vitro and In-Vivo In ammatory Bowel Disease

Jagadish Koya Saint John's University: St John's University Tong Shen Regor Therapeutics Group Geming Lu Icahn School of Medicine at Mount Sinai Alex Gauthier Saint John's University: St John's University Lin Mantell Saint John's University: St John's University Charles Ashby Saint John's University: St John's University Sandra Reznik (  rezniks@stjohns.edu ) Saint John's University: St John's University https://orcid.org/0000-0002-6272-7198
Efficiency of Energy Conversion in Underwater Spark Discharges and Associated Bubble Oscillations: Experimental Results

In this paper low-voltage high-energy spark discharges in water and associated bubble oscillations are studied experimentally. Pressure waves emitted during spark discharges and during following bubble oscillations have been recorded and analyzed to determine the radiated acoustic energy and potential energy of the bubbles. These energies are then used to determine the efficiency of energy conversion. For example, it has been found out that only 2% to 8% of electrical energy stored in the capacitor bank is converted into the potential energy of the bubble at its first maximum volume and only about 30% of this potential energy is radiated as acoustic energy in the first bubble pulse. Due to specific features of the low-voltage apparatus used, bubbles of different sizes have been generated and these bubbles were found to oscillate with different intensities. To explain this great diversity in the generated bubbles, the electrical circuit of the apparatus and the early stages of the spark discharge are analyzed. While it is confirmed that the bubble size grows with the energy used for discharge, the relation between the intensity of bubble oscillations and the circuit parameters is more complex. To explain this relation, two hypotheses, partly supported by the data available, are presented. The first hypothesis concerns the rate of electrical energy delivery into the discharge channel, and the second hypothesis concerns the efficiency of electrical energy conversion into heat in the discharge channel.
Freezability of Goat Epididymal Sperm using Aloe vera Extract and Trehalose in Diluents

Background: Cryopreservation process causes oxidative stress on sperm membranes, which in turn damages sperm organs and enzymatic activities which thereby decrease motility, functional membrane integrity and sperm fertility. Therefore, current study carried out to evaluate the effect of Aloe vera Ethanolic Extract (AEE), alone and with trehalose in diluents on cryopreserved epididymal goat sperm. Methodology: Epididymal sperm isolated from testes with motility >70% and total morphological abnormalities <10%. The experimental treatments consist of control (no additives) and basic diluents plus 5, 10, 20 or 50 µg/ml of AEE (AEE1, AEE2, AEE3 and AEE4, respectively), tr (150 mM trehalose), tr+AEE1, tr+AEE2, tr+AEE3 and tr+AEE4. Results: Obtained data show that the extender containing AEE3, AEE1+tr, AEE2+tr and AEE3+tr improved significantly the cryopreserved sperm. The combined treatments indicate also a decrease in MDA than control. In addition, AEE2+tr and AEE3+tr showed the lowest (P<0.05) DNA fragmentation compared to the other treatments. Extender containing AEE3+tr resulted in higher total motility and viability than the extender containing tr alone, as well as AEE1, AEE2 and AEE4 treatments. Conclusion: The present study indicates that ethanolic extract of Aloe vera could be used for goat sperm cryopreservation. Also, it can be concluded that trehalose in combination with 20 µg/ml of Aloe vera extract can be promised cryoprotectant in goat epididymal sperm freezing.
Dietary nano-dicalcium phosphate improves immune response and intestinal morphology of broiler chickens

Various levels of substitution of nano-dicalcium phosphate (nCaHPO4) for the calcium and phosphorus supplied by limestone and monocalcium phosphate were evaluated, based on weight change, serum constituents, immune response and intestinal morphology of broiler chickens. The birds were assigned to five dietary treatments containing 0% (control), 40%, 60%, 80%, and 100% substitution levels of nCaHPO4 from days 0 to 42. Data were collected for blood measurement and intestinal morphology and analysed with one-way analysis of variance. Feeds substituted with 40% nCaHPO4 showed a higher mean value of final weight and better feed conversion. The results revealed similar haematological parameters across the treatment groups. At the same time, nCaHPO4 influenced most of the serum biochemical constituents, such as calcium, phosphorus, glucose, albumin, and total protein. High concentrations of total protein were observed for birds fed 60% nCaHPO4 compared with other treatments. The highest comparable means for albumin were observed in birds fed 40% and 60% nCaHPO4. Cholesterol levels were increased when birds were fed 0% and 40% of diets. Elevated serum phosphorus concentration was observed when 0%, 40%, 60% and 100% substitution levels were fed, whereas 80% presented the least mean value. The results also showed that birds fed 40% nano-dicalcium phosphate had the highest villi length, which signified improved absorption of minerals. In conclusion, 40% and 60% levels of nCaHPO4 could be used to boost immune response and functional intestinal morphology of broiler chicken with no negative effect on haematological parameters.
Static and dynamic analysis of multisection and multielectrode semiconductor lasers

In this paper we introduce a new laser diode simulator for general multisection and multielectrode structures. Lasing mode features like linewidth, intensity and frequency modulation responses, etc., are calculated considering all the major physical effects. Furthermore, this model allows us to evaluate thermal effects in order to obtain more realistic results also at high injection rates.
An integrated model of supply chain performance evaluation and decision-making using structural equation modelling and fuzzy AHP

This paper aims to propose a comprehensive evaluation tool and decision model, which can help the practitioners to gauge their supply chain performance and guide them in decision-making for further improvements. The model provides a salient notion of integrated supply chain performance evaluation approach distilled from the literature. The five important performance evaluation criteria (supply chain planning performance, supply chain partnership performance, production performance, delivery and logistic performance and customer service and satisfaction performance) and corresponding 19 sub-criteria have been identified. An integrated methodology of structural equation modelling (SEM) and fuzzy analytic hierarchy process (FAHP) have been applied to the proposed model to a real case study of Indian textile–apparel–retail supply chain network.
Automation of an electron cyclotron resonance plasma system for the manufacturing of inhomogeneous optical coatings for sensor applications

The control system for an electron cyclotron resonance plasma enhanced chemical vapour deposition (ECR-PECVD) reactor was developed for the growth of inhomogeneous dielectric filters for optical applications. The system is intended to supervise the growth of films of a required refractive index profile on different substrates including fibre ends. It controls the gas flow rates, microwave power, RF substrate bias and the ECR and extraction magnet power supplies. All controlled parameters are displayed in real time as graphs and tables. The system also tunes the microwave generator to minimize reflected power during deposition. This is required because the varying gas flow rates and gas composition change the plasma conditions. The control system consisting of software and hardware, is implemented on an ECR-PECVD system that was designed and built in our laboratory. The characteristics of the ECR-PECVD-system and the automation of the deposition process is discussed using the growth of a complex rugate optical filter as an example.
Churn Influence Diffusion in a Multi-Relational Call Network

Customer Churn is a pesky problem that continues to haunt telecommunication companies. Social influence analysis has recently been introduced in churn prediction, motivated by the fact thatsome users can churn due to accumulated churn influence that they’ve received from other churners.We’ve collected call data records of about 10 thousands mobile phone users of one the largest mobile network operators in China,and have built a Multi-relational call network which is a graph constructed by mobile phone users considered as nodes and the interactive calls between them considered as the relationships or edges. We’veapplied Linear Threshold (LT) to model the diffusion of churners influence in theobtainedsocial network, in order to analyze the relevance of social affinities in the diffusion of churn influence.The results indicate that churn influence diffusion depends not only on the number of initial churners but also on the existingaffinities between users.
Bioaerosols in the Amazon rain forest: temporal variations and vertical profiles of Eukarya, Bacteria, and Archaea

Abstract. The Amazon rain forest plays a major role in global hydrological cycling, and biogenic aerosols are likely to influence the formation of clouds and precipitation. Information about the sources and altitude profiles of primary biological aerosol particles, however, is sparse. We used fluorescence in situ hybridization (FISH), a molecular biological staining technique largely unexplored in aerosol research, to investigate the sources and spatiotemporal distribution of Amazonian bioaerosols on the domain level. We found wet season bioaerosol number concentrations in the range of 1–5 × 105 m−3 accounting for > 70 % of the coarse mode aerosol. Eukaryotic and bacterial particles predominated, with fractions of ∼ 56 % and ∼ 26 % of the intact airborne cells. Archaea occurred at very low concentrations. Vertical profiles exhibit a steep decrease in bioaerosol numbers from the understory to 325 m height on the Amazon Tall Tower Observatory (ATTO), with a stronger decrease in Eukarya compared to Bacteria. Considering earlier investigations, our results can be regarded as representative for near-pristine Amazonian wet season conditions. The observed concentrations and profiles provide new insights into the sources and dispersion of different types of Amazonian bioaerosols as a solid basis for model studies on biosphere–atmosphere interactions such as bioprecipitation cycling.
A KSAO Based Solvers’ Competency Model in Crowdsourcing Contests

As typical crowdsourcing innovation, crowdsourcing contests have the characteristics such as clear task objectives, intense timelines, and voluntary participation of solvers. It is critical for improving crowdsourcing contests' efficiency to evaluate solvers' competency scientifically and identify appropriate solvers rapidly. The competency model was constructed from four dimensions of Knowledge , Skill , Ability , and Others . This framework will benefit not only the solvers but also the seekers and the crowdsourcing platform
Transformation of Value in Innovative Business Models: The Case of Pharmaceutical Market

Creating and developing innovative business models (BM) is currently one of the key success factors for contemporary business. Rapid complex changes in the world reemphasize the need to better understand how BM can be successfully innovated in different markets. The digital component of BM innovation comes under a special spotlight, using the example of a company within the pharmaceutical industry. In particular, this study demonstrates how BM innovation can be developed and implemented in practice within the pharmaceutical market, which accelerates its digital transformation to increase the value it brings to the healthcare systems around the world while sustaining the ongoing crisis. In order to do that, the current paper offers a framework for BM innovation that defines BM elements, BM innovation aspects, and BM innovation logic. The study covers six markets that represent different value creation systems and mechanisms. This paper demonstrates how technological innovations can be activated using managerial tools and insights and also how they can be combined into the holistic system based on the needs of the key value chain actors.
INCORPORATING POULTRY LITTER IN RABBIT FEED: A SOLID WASTE MANAGEMENT STRATEGY

SUMMARY An investigation was carried out for recycling of poultry litter by incorporating it in animal feed to reduce the risk of polluting the environment and reduce the cost of feed. Twenty-four 2 – 3 months old growing rabbits (Chinchilla breed) were used to evaluate the nutritive quality of poultry litter (PL). Soy Bean Meal (SBM) in the diets was replaced with poultry litter at 0,5,10 and 15% (21.70, 48.4, and 65.2%) levels, respectively in a study that lasted twelve weeks. The rabbits were divided into four groups and each group was assigned to one of the four dietary treatments in a Completely Randomized Block Design (CRBD). Results obtained showed that the inclusion of poultry litter had significant (P<0.05) effects on mean weight gain, feed intake and feed efficiency. These indices were better on 0% (control) and 5% when compared to 10% and 15% levels of inclusion. The economics of production indicated a better performance on those fed poultry litter over the control group in all the indices vis-a-vis cost/kg feed (N), cost/kg weight gain (N) and relative cost (%) measured suggesting that poultry litter has an economic advantage over soy bean meal as protein supplement in diet formulation. From the results obtained, poultry litter could replace up to 5% (21.70) of soy bean meal in growing rabbits diet without detrimental effects on growth performance. This study justifies the practical possibility of using poultry litter as dietary protein source using rabbits as a model and also provides an environmentally friendly way of disposing this pollutant.
In-line single-mode wavelength division multiplexer/demultiplexer

A single-mode fibre optic wavelength division multiplexer/demultiplexer at 1.32 ? m and 1.55 ? m, constructed from a fused evanescent wave coupler, is described. This device has an insertion loss of less than 0.05 dB and a wavelength isolation of 16 dB at 1.32 ? m and 18 dB at 1.55 ? m.
The effect of molecular structure on prebreakdown phenomena in dielectric liquids under a nonuniform field

In the present work, prebreakdown phenomena in dielectric liquids which have various molecular structure, especially those with chlorine or fluorine atoms and some double bonds included in their molecular formula, are observed in detail for positive and negative point polarities, under the application of an impulse voltage (1.1/225 µs) to the point electrode. As test liquids, cyclic hydrocarbon liquids such as cyclohexane, benzene, chlorocyclohexane, monochlorobenzene, hexafluorobenzene, and cyclohexene are used. Results obtained in these liquids are compared with those obtained in straight‐chain hydrocarbon liquids.
Analysis of the aftertreatment performance in HD-SI engine fueled with LPG

This research/article aimed to analyze the influence of an after-treatment system (ATS) on emissions of a heavy-duty spark-ignition (HD-SI) engine fueled with liquified petroleum gas (LPG), in the context of current Euro VI emissions requirements. The ATS is composed by a three-way catalyst (TWC) in series with a diesel particle filter (DPF). Emissions testing were carried out on an engine test bench according to homologation procedures, performing both world harmonized stationary cycle (WHSC) and world harmonized transient cycle (WHTC), to study the effects of the engine operating parameters on pollutant emissions behavior and ATS performance during steady and dynamic states, respectively. Instruments used were a gas analyzer Horiba MEXA ONE to measure gaseous emissions, HORIBA OBS ONE PN to measure particle matter (PM) concentration, and spectrometer TSI EEPS 3090 to measure PM concentration and particle size distribution (PSD). The results showed some important aspects such as the effects of engine speed and load on pollutant emissions formation and ATS performance, the influence of the three-way catalyst (TWC) on particulate matter (PM) reduction due to the relationship between volatile unburned hydrocarbons (UHC) and the emergence of nucleation-mode particles, stressing that ATS implementation is mandatory to meet the current emissions requirements.
Monolithically integrated 64-channel WDM channel selector with novel configuration

A novel configuration, which can reduce the number of gates in a wavelength division multiplexing (WDM) channel selector, is proposed. A monolithically integrated 64-channel selector is realised with only 16 optical gates. Selecting operation for all 64 channels was confirmed and zero insertion loss was achieved for almost all channels.
Mobile mapping system for the automated detection and analysis of road delineation

This study will explore a low-cost mobile mapping system that has been developed to carry out road delineation surveys. This data acquisition system has been designed to be fully compatible for both mounting and calibration on any vehicle possessing a set of standard roof bars. This system, constructed inside a standard roof box, is fully self-contained and powered making it completely independent from the carrier vehicle. In order to allow ease of use by both field engineers and untrained personnel, user-friendliness was prominent in the design of both the hardware and the user interface. Simplified calibration procedures reduce set-up times and again allow untrained personnel to initialise the hardware. Two applications have been developed for this system. Automated raised pavement marker detection allows for the mapping of both functioning and non-functioning road studs using stereo reconstruction coupled with navigation data. A road line detection algorithm has also been developed to detect and extract road line data from captured images with a view to both assessing retroreflectivity and mapping these data for maintenance planning.
Driving characteristics of an electric vehicle system with independently driven front and rear wheels

A new type electric vehicle drive system is proposed which has suitable functions for an urban car to easily cope with common situations such as traffic jams, and starting and stopping operations, and the special situation of failure of the motor drive system while running on city routs. The proposed electric vehicle drive system has a permanent magnet synchronous motor (SM) for the front wheel drive and an induction motor (IM) for the rear wheel drive. Various functions have been verified through simulations and experiences with an experimental driving apparatus equivalent to the actual system.