Miracle-dz/UniFAR-FTUs huggingface dataset:
Tomato Disease Classiﬁcation using AlexNet

—This work employs the AlexNet architecture for tomato diseases detection. The experimental setup is divided into two categories: In the ﬁrst case a 10-class classiﬁer is trained with healthy (1 class) and diseased (9 classes) leaf images and in the second case 9 different 2-class classiﬁers are trained with healthy (1 class) and diseased (1 class) leaf image. All cases are tested for three training scenarios. Finally, we point a particular feature of the PlantVillage dataset.
The role of E2A in ATPR‐induced cell differentiation and cycle arrest in acute myeloid leukaemia cells

Acute myeloid leukaemia (AML) is a biologically heterogeneous disease with an overall poor prognosis; thus, novel therapeutic approaches are needed. Our previous studies showed that 4‐amino‐2‐trifluoromethyl‐phenyl retinate (ATPR), a new derivative of all‐trans retinoic acid (ATRA), could induce AML cell differentiation and cycle arrest. The current study aimed to determine the potential pharmacological mechanisms of ATPR therapies against AML. Our findings showed that E2A was overexpressed in AML specimens and cell lines, and mediate AML development by inactivating the P53 pathway. The findings indicated that E2A expression and activity decreased with ATPR treatment. Furthermore, we determined that E2A inhibition could enhance the effect of ATPR‐induced AML cell differentiation and cycle arrest, whereas E2A overexpression could reverse this effect, suggesting that the E2A gene plays a crucial role in AML. We identified P53 and c‐Myc were downstream pathways and targets for silencing E2A cells using RNA sequencing, which are involved in the progression of AML. Taken together, these results confirmed that ATPR inhibited the expression of E2A/c‐Myc, which led to the activation of the P53 pathway, and induced cell differentiation and cycle arrest in AML.
The urinary exosomes derived from premature infants attenuate cisplatin-induced acute kidney injury in mice via microRNA-30a-5p/ mitogen-activated protein kinase 8 (MAPK8)

ABSTRACT Acute kidney injury (AKI) is a susceptible factor for chronic kidney disease (CKD). There is still a lack of effective prevention methods in clinical practice. This study investigated the protective effect of the urinary exosomes from premature infants on cisplatin-induced acute kidney injury. Here we isolated exosomes from the fresh urine of premature infants. A C57BL/6 mice model of cisplatin-induced acute kidney injury was given 100 ug urinary exosomes 24 hours after model establishment. The kidneys were collected for pathological examination and the evaluation of renal tubular damage and apoptosis. In the in vitro experiment, human renal cortex/proximal tubular cells (HK-2) were induced by cisplatin to assess the effect of the urine exosomes from premature infants. Exosome microRNA (miRNA) sequencing technology was applied to investigate the miRNAs enriched in exosomes and the dual-luciferase gene reporter system to examine the targeting relationship of the miRNA with target genes. The results indicated that the urinary exosomes could decrease the serum creatinine level and the apoptosis of renal tubular cells, and reduce mice mortality. In addition, miR-30a-5p was the most abundant miRNA in the exosomes. It protected HK-2 cells from cisplatin-induced apoptosis by targeting and down-regulating the mitogen-activated protein kinase 8 (MAPK8). Together, our findings identified that the urinary exosomes derived from premature infants alleviated cisplatin-induced acute kidney injury and inhibited the apoptosis of HK-2 via miR-30a-5p, which could target MAPK8. These findings implied that urinary exosomes from premature infants riched in miR-30a-5p might become a potential treatment for AKI.
Potensi Modal Sosial Pada Budaya Lokal Dalam Pembangunan Daerah

Pembahasan tema ini akan mengantar kita pada suatu pengertian tantang jaringan hubungan antar individu dalam masyarakat dan jaringan hubungan antar kelompok. Jaringan-jaringan itu dibimbing oleh suatu norma atau aturan, agar jaringan tersebut teratur dan terarah pada tujuan yang dikehendaki oleh kelompok Sosial menurut kebutuhan dan kepentingannya. Dalam norma dan jaringan itu, terlihat interaksi Sosial dan kemitraan yang saling mengukuhkan dan menguntungkan. Disinilah pentingnya modal Sosial disadari kegunaannya dan berpotensi mengantarkan manusia melakukan sector-sektor kehidupannya. Tenunan jaringan Sosial itu yang berpedoman pada aturan dan peraturan yang dibuatnya, maka tampak adanya sistem-sistem yang pada bentuknya yang lebih abstrak, adalah sebuah struktur Sosial. Jaringan-jaringan kerja dengan aturan tertentu, biasanya berwujud pada sebuah lembaga Sosial. Dalam lembaga terjadi tindakan Sosial dan interaksi antar individu dan kelompok untuk memenuhi harapan, kebutuhan, kepentingan dan tujuan lembaga. Dengan demikian, modal Sosial berisi lembagalembaga Sosial, etika Sosial dan etika lingkungan, sementara kearifan local merupakan bagian sempit dari modal Sosial, sedang etos Sosial merupakan sifat, sikap, watak dan karakter Sosial yang bersesuai dengan world view masyarakat local tersebut.
The role of junctophilin proteins in cellular function

Junctophilins (JPHs) comprise a family of structural proteins that connect the plasma membrane to intracellular organelles such as the endo/sarcoplasmic reticulum. Tethering of these membrane structures results in the formation of highly organized subcellular junctions that play important signaling roles in all excitable cell types. There are four JPH isoforms, expressed primarily in muscle and neuronal cell types. Each JPH protein consists of 6 'membrane occupation and recognition nexus' (MORN) motifs, a joining region connecting these to another set of 2 MORN motifs, a putative alpha-helical region, a divergent region exhibiting low homology between JPH isoforms, and a carboxy-terminal transmembrane region anchoring into the ER/SR membrane. JPH isoforms play essential roles in developing and maintaining subcellular membrane junctions. Conversely, inherited mutations in JPH2 cause hypertrophic or dilated cardiomyopathy, while trinucleotide expansions in the JPH3 gene cause Huntington Disease-Like 2. Loss of JPH1 protein levels can cause skeletal myopathy, while loss of cardiac JPH2 levels causes heart failure and atrial fibrillation, among other disease. This review will provide a comprehensive overview of the JPH gene family, phylogeny, and evolutionary analysis of JPH genes and other MORN domain proteins. JPH biogenesis, membrane tethering, and binding partners will be discussed, as well as functional roles of JPH isoforms in excitable cells. Finally, potential roles of JPH isoform deficits in human disease pathogenesis will be reviewed.
Nonmydriatic Retinal Photography in the Outpatient Neurology Resident Clinic

Background: The funduscopic examination is an essential component of the neurologic examination. However, examination of the ocular fundus with a direct ophthalmoscope is often difficult. Nonmydriatic ocular fundus photography allows direct visualization of the ocular fundus with high-quality photographs. We used nonmydriatic ocular fundus photography to improve patient care and funduscopy skills of residents in the Neurology Resident Clinic. Methods: At the time of triage, funduscopic photographs of all new neurology resident clinic patients were taken. The images were imported into the hospital's imaging software. The residents completed a full neurologic examination, including a funduscopic examination with a handheld ophthalmoscope. At the time of staffing the patients with the attendings, the residents received immediate feedback and teaching on retina photograph evaluation. Results: A total of 255 patients were enrolled. Of those, 230 (90%) had at least one high-quality funduscopic photograph. Retinal photographs were normal in 161 (70%). Out of the 69 abnormal photographs, only 7% of abnormalities were detected by the residents. Ninety-three percent of residents found the retinal photographs useful. Conclusions: Nonmydriatic ocular fundus photography improved the care in patients presenting to a Neurology resident clinic and facilitated residents in recognizing funduscopic findings. Its benefits are clear when one considers (1) the high risk of negative patient outcomes and possible medicolegal consequences due to missed findings, (2) the ease of incorporating retinal photographs into the patients' medical records, and (3) the benefit of improving resident education in regard to the ophthalmologic examination.
Chromodomain Helicase DNA Binding Protein 1-like, a negative regulator of Forkhead box O3a, promotes the proliferation and migration of Angiotensin II-induced vascular smooth muscle cells

ABSTRACT Essential hypertension (EH) represents a major risk factor for stroke, myocardial infarction, and heart failure. Dysregulated proliferation and migration of vascular smooth muscle cells (VSMCs) play an important role in pathogenesis of EH. This study aims to investigate the effect of Chromodomain Helicase DNA Binding Protein 1-Like (CHD1L) on Angiotensin II (AngII)-induced VSMCs injury and reveal the underlying mechanism. The expression of CHD1L in EH patients was determined by bioinformatics analysis, and then it was silenced in AngII-induced VSMCs to detect the changes in cellular functions including proliferation, migration, invasion and phenotypic switching via CCK-8, EDU staining, wound healing, transwell and Western blot assays, respectively. Inflammation and oxidative stress were also measured by detecting related markers via commercial kits. After confirming the binding sites between forkhead box O3A (FOXO3a) and CHD1L and their negative association by bioinformatics analysis, FOXO3a was further silenced, and the cellular functions were assessed again to reveal the underlying mechanism. Results showed that CHD1L was highly expressed in EH, and interference of CHD1L suppressed the proliferation, migration, invasion and phenotypic switching in VSMCs. Inflammation and oxidative stress were also restrained by CHD1L knockdown. After validating the negative role of FOXO3a in regulating CHD1L, it was found that FOXO3a abrogated the effect of CHD1L knockdown on the cellular functions of AngII-induced VSMCs. In conclusion, FOXO3a suppresses the proliferation and migration of AngII-induced VSMCs by down-regulating CHD1L.
Aging and Vascular Compliance in Hypertensive Patients Mirrored in Routine Investigations

The global prevalence of arterial hypertension is increasing. Due to its consequences, hypertension is a leading cause of morbidity /mortality. In this regard, awareness of the importance of diagnosis as well as proper treatment of hypertension is mandatory. Objectives: This study aims to analyze vascular compliance and different pressure characteristics depending on age, along with highlighting some clinical-paraclinical correlations in people diagnosed with hypertension. We used usual laboratory analyzes and the blood pressure values obtained using ABPM/24 hr, in addition to highlighting the impact of age on those parameters. Materials and methods: The sample comprises of 99 adults diagnosed with hypertension. We divided them into 2 groups, according to age: cases sample: 52 patients (age> 60 years old) and control sample: 47 patients (age< 60 years old). Results: In the control group, RDW was correlated with the general and diurnal systolic and diastolic hypertonicity indexes and the platelet count was positively correlated only with the morning surge. The dipping profile correlates negatively with RDW. In the elderly, all three systolic blood pressure parameters are influenced by ESR and also correlate negatively with HDL, with an inverse proportional relationship. Statistically significant correlations were found between blood glucose and diurnal systolic and diastolic hypertonicity indexes. Mean Pulse Pressure correlates in the elderly with HDL (negative correlations) and in the young with RDW (positive correlations). Conclusions: Vascular compliance can be estimated with the help of HDL in the elderly and RDW in young people, considering the influence of the two parameters on pulse pressure.
Prehospital Manual Ventilation: An NAEMSP Position Statement and Resource Document

Abstract Manual ventilation using a self-inflating bag device paired with a facemask (bag-valve-mask, or BVM ventilation) or invasive airway (bag-valve-device, or BVD ventilation) is a fundamental airway management skill for all Emergency Medical Services (EMS) clinicians. Delivery of manual ventilations is challenging. Several strategies and adjunct technologies can increase the effectiveness of manual ventilation. NAEMSP recommends: All EMS clinicians must be proficient in bag-valve-mask ventilation. BVM ventilation should be performed using a two-person technique whenever feasible. EMS clinicians should use available techniques and adjuncts to achieve optimal mask seal, improve airway patency, optimize delivery of the correct rate, tidal volume, and pressure during manual ventilation, and allow continual assessment of manual ventilation effectiveness.
Caffeic acid phenethyl ester induces radiosensitization via inhibition of DNA damage repair in androgen‐independent prostate cancer cells

In the present study, we evaluated the radiomodulatory potential of caffeic acid phenethyl ester (CAPE), an active component of traditional herbal medicine propolis. CAPE has been identified as a potent anticancer agent in multiple cancer types and is reported to have the dual role of radioprotection and radiosensitization. However, the radiomodulatory potential of CAPE in prostate cancer (PCa), which eventually becomes radioresistant is not known. Therefore, we studied the effect of co‐treatment of CAPE and gamma radiation on androgen‐independent DU145 and PC3 cells. The combination treatment sensitized PCa cells to radiation in a dose‐dependent manner. The radiosensitizing effect of CAPE was observed in both cell lines. CAPE enhanced the level of ionizing radiation (IR)‐induced gamma H2AX foci and cell death by apoptosis. The combination treatment also decreased the migration potential of PCa cells. This was confirmed by increased expression of E‐cadherin and decrease in vimentin expression. CAPE sensitized PCa cells to radiation in vitro and induced apoptosis, augmented phosphorylation of Akt/mTOR, and hampered cell migration. At the mechanistic level, co‐treatment of CAPE and IR inhibited cell growth by decreasing RAD50 and RAD51 proteins involved in DNA repair. This resulted in enhanced DNA damage and cell death. CAPE might represent a promising new adjuvant for the treatment of hormone‐refractory radioresistant PCa.
On the validation of fluid plasma model for pulsed DBD plasma actuator simulations against full kinetic approach

Dielectric barrier discharge plasma (DBD) actuators have demonstrated high potential for active flow control for both subsonic and supersonic applications [1-5]. However, both experimental and numerical studies showed that the performance of DBD plasma actuators is rather sensitive to the DBD parameters, such as driving voltage and position of the actuators on the aerodynamic body. In order to start transition between research and development efforts to the implementation into the existing platforms, it is important to gain confidence in both experimental and simulation results for DBD laboratory studies.
Empagliflozin protects glomerular endothelial cell architecture in experimental diabetes through the VEGF‐A/caveolin‐1/PV‐1 signaling pathway

In addition to having blood glucose‐lowering effects, inhibitors of sodium glucose cotransporter 2 (SGLT2) afford renoprotection in diabetes. We sought to investigate which components of the glomerular filtration barrier could be involved in the antiproteinuric and renoprotective effects of SGLT2 inhibition in diabetes. BTBR (black and tan, brachyuric) ob/ob mice that develop a type 2 diabetic nephropathy received a standard diet with or without empagliflozin for 10 weeks, starting at 8 weeks of age, when animals had developed albuminuria. Empagliflozin caused marked decreases in blood glucose levels and albuminuria but did not correct glomerular hyperfiltration. The protective effect of empagliflozin against albuminuria was not due to a reduction in podocyte damage as empagliflozin did not affect the larger podocyte filtration slit pore size nor the defective expression of nephrin and nestin. Empagliflozin did not reduce the thickening of the glomerular basement membrane. In BTBR ob/ob mice, the most profound abnormality seen using electron microscopy was in the endothelial aspect of the glomerular capillary, with significant loss of endothelial fenestrations. Remarkably, empagliflozin ameliorated the subverted microvascular endothelial ultrastructure. Caveolae and bridging diaphragms between adjacent endothelial fenestrae were seen in diabetic mice and associated with increased expression of caveolin‐1 and the appearance of PV‐1. These endothelial abnormalities were limited by the SGLT2 inhibitor. Although no expression of SGLT2 was found in glomerular endothelial cells, SGLT2 was expressed in the podocytes of diabetic mice. VEGF‐A, which is a known stimulus for endothelial caveolin‐1 and PV‐1, was increased in podocytes of BTBR ob/ob mice and normalized by SGLT2 inhibitor treatment. Thus, empagliflozin’s protective effect on the glomerular endothelium of diabetic mice could be due to a limitation of the paracrine signaling of podocyte‐derived VEGF‐A that resulted in a reduction of the abnormal endothelial caveolin‐1 and PV‐1, with the consequent preservation of glomerular endothelial function and permeability. © 2022 The Pathological Society of Great Britain and Ireland.
Quantifying Gender Bias in Consumer Culture

Cultural items like songs have an important impact in creating and reinforcing stereotypes, biases, and discrimination. But the actual nature of such items is often less transparent. Take songs, for example. Are lyrics biased against women? And how have any such biases changed over time? Natural language processing of a quarter of a million songs over 50 years quantifies misogyny. Women are less likely to be associated with desirable traits (i.e., competence), and while this bias has decreased, it persists. Ancillary analyses further suggest that song lyrics may help drive shifts in societal stereotypes towards women, and that lyrical shifts are driven by male artists (as female artists were less biased to begin with). Overall, these results shed light on cultural evolution, subtle measures of bias and discrimination, and how natural language processing and machine learning can provide deeper insight into stereotypes and cultural change.
When are Collaborative Interventions for a More Sustainable Agriculture Successful? Towards an Analytical Framework

Today's agriculture both contributes significantly to current environmental, social, and economic problems and also suffers from the consequences of this non- sustainable development. Despite the importance of research at the farm level to tackle these problems, it has often been argued that research and work for sustainable agricul- ture has to go beyond the farm gate. However, designing and implementing solutions at higher levels makes the collaboration of different stakeholders indispensable. There has already been much work on conditions influencing success or failure of joint action but there has been no research specifically on conditions for the success of collaborative in- terventions that aim at the improvement of the sustainability of agriculture. Further- more, much of the existing work is based on the examination of one or few case studies, which makes it difficult to identify overall patterns. To fill this gap, I am developing an analytical scheme that can be used for comparative, integrative analysis. In this paper, I describe how the variables making up this analytical scheme were derived. This in- cludes the formulation of a meaningful definition of what actually is a case as well as the operationalization of 'success'. Finally, I give an overview over the resulting coding scheme, containing factors that potentially contribute to or hinder success of collabora- ti ve interventions trying to achieve a more sustainable agriculture.
Review—"Knees" in Lithium-Ion Battery Aging Trajectories

Lithium-ion batteries can last many years but sometimes exhibit rapid, nonlinear degradation that severely limits battery lifetime. Here, we review prior work on “knees” in lithium-ion battery aging trajectories. We first review definitions for knees and three classes of “internal state trajectories” (termed snowball, hidden, and threshold trajectories) that can cause a knee. We then discuss six knee “pathways”, including lithium plating, electrode saturation, resistance growth, electrolyte and additive depletion, percolation-limited connectivity, and mechanical deformation, some of which have internal state trajectories with signals that are electrochemically undetectable. We also identify key design and usage sensitivities for knees. Finally, we discuss challenges and opportunities for knee modeling and prediction. Our findings illustrate the complexity and subtlety of lithium-ion battery degradation and can aid both academic and industrial efforts to improve battery lifetime.
The impact of seasonality on service quality in beach hotels : the case of the Palm Beach Hotel and Bungalows

One of the biggest issues that any business is facing nowadays is offering the highest quality of service possible to its clients. Having acknowledged the imminent importance of offering high quality of service in the Cypriot hotel industry, this report went on to investigate the impact that seasonality has on the quality of service in beach hotel. In order to do so in a feasible way and taking into consideration the limitation of the study, this report selected the case study method and used the Palm Beach Hotel and Bungalows (Four stars hotel) who acted as the sponsor to it. The hotel is situated right on the beach of the Larnaca bay in Cyprus, just off Dhekelia road and 10 minutes by car from the center of the city of Larnaca. With the aid of semi structure questionnaires and self filled questionnaires in two indicative periods (Winter and Summer) as well as the extensive use of literature review this report managed to collect a great number of data that allowed it to produce some solid recommendations that if followed by the sponsor hotel will bring actual change to the organization thus allowing it to offer the highest quality of service possible to its guests all year round. These recommendations aimed at improving both the tangible and the intangible aspects of the quality of service that the hotel has to offer. Regarding the tangible aspects, amongst others, this study went on to suggest the total renovation of the hotel and addition of new facilities. Moreover there were some more suggestions that included the intangible aspects related to the hotel’s day to day operations and they included a number of Staff related issues related to the hiring, induction and training of new staff members as well as the creation of a quality assurance office headed by the hotel’s quality assurance manager. Furthermore this research went on even further and suggested some secondary recommendations related to the mitigation of the problem of seasonality that were directed towards the tourism policy makers of Cyprus, namely the Government of the Republic of Cyprus. Finally, it also has to be mentioned that the framework developed for the purposes of this research could be adopted and used by other researchers in hotels of different types and classifications.