Miracle-dz/UniFAR-FTUs huggingface dataset:
A seed‐specific transcription factor, HSFA9, anticipates UV‐B light responses by mimicking the activation of the UV‐B receptor in tobacco

SUMMARY Sunflower heat shock factor A9 (HSFA9, hereafter A9) is a transcription factor involved in seed desiccation tolerance and longevity. A9 also links the regulation of seed maturation with that of seedling photomorphogenesis through visible light receptors. Analyses in transgenic Nicotiana tabacum (tobacco) indicated that A9 also affects responses mediated by NtUVR8, the receptor of ultraviolet light B (UV‐B). We compared the effects of A9 and UV‐B illumination on the nuclear localization of GFP‐NtUVR8 in Nicotiana benthamiana leaves. We also used co‐immunoprecipitation and limited proteolysis for analyzing the interaction between A9 and NtUVR8. We found that A9, by binding to NtUVR8, induced structural changes that resulted in enhancing the nuclear localization of NtUVR8 by hindering its nuclear export. The localization of UVR8 is crucial for receptor activation and function in Arabidopsis, where UV‐B‐activated nuclear UVR8 binds the E3 ubiquitin ligase COP1, leading to enhanced UV‐B responses and photoprotection. A9 similarly activated NtUVR8 by enhancing COP1 binding without UV‐B light. Seedlings and dark‐germinated seeds that overexpress A9 showed primed UV‐B light stress protection. Our results unveil a UV‐B‐independent activation mechanism and a role for UVR8 in plant seeds that might contribute to early stress protection, facilitating seedling establishment.
The complexity of ventral CA1 and its multiple functionalities

The hippocampus is one of the most widely investigated brain regions with its massive contributions to multiple behaviours. Especially, the hippocampus is subdivided into the dorsal and ventral parts playing distinct roles. In this review, we will focus on the ventral hippocampus, especially the ventral CA1 (vCA1), whose role is being actively discovered. vCA1 is well known to be associated with emotion‐like behaviour, in both positive (reward) and negative (aversive) stimuli. How can this small region in volume mediate such variety of responses? This question will be answered with technologies up to date that have allowed us to study in‐depth the specific neural circuit and to map the complex connectivity.
``What is a Super Mario level anyway?'' An Argument For Non-Formalist Level Generation in Super Mario Bros.

The video game series Super Mario Bros. has proven immensely popular in the field of artificial intelligence research within the last 10 years. Procedural content generation research in Super Mario continues to prove popular to this day. However much of this work is based largely on the notions of creating ‘Mario-like’ level designs, patterns or structures. In this paper, we argue for the need to diversify the generative systems used for level creation within the Super Mario domain through the introduction of more aesthetic-driven and ‘non-formalist’ approaches towards game design. We assess the need for a broader approach to automated design of Mario artefacts and gameplay structures within the context of Super Mario Maker : a Super Mario Bros. level creation tool. By assessing a number of top-ranked levels established within the player community, we recognise a populist movement for more radical level design that the AI community should seek to embrace.
“Dragons” on the landscape: Modeling the abundance of large carnivorous dinosaurs of the Upper Jurassic Morrison Formation (USA) and the Upper Cretaceous Dinosaur Park Formation (Canada)

Counts of the number of skeletal specimens of “adult” megaherbivores and large theropods from the Morrison and Dinosaur Park formations—if not biased by taphonomic artifacts—suggest that the big meat‐eaters were more abundant, relative to the number of big plant‐eaters, than one would expect on the basis of the relative abundance of large carnivores and herbivores in modern mammalian faunas. Models of megaherbivore population density (number of individuals per square kilometer) that attempt to take into account ecosystem productivity, the size structure of megaherbivore populations, and individual megaherbivore energy requirements, when combined with values of the large theropod/megaherbivore abundance ratio, suggest that large theropods may have been more abundant on the landscape than estimates extrapolated from the population density versus body mass relationship of mammalian carnivores. Models of the meat production of megaherbivore populations and the meat requirements of “adult” large theropods suggest that herbivore productivity would have been insufficient to support the associated number of individuals of “adult” large theropods, unless the herbivore production/biomass ratio was substantially higher, and/or the large theropod meat requirement markedly lower, than expectations based on modern mammals. Alternatively, or in addition to one or both of these other factors, large theropods likely included dinosaurs other than megaherbivores as significant components of their diet.
Machine Learning Analysis of Handgun Transactions to Predict Firearm Suicide Risk

Key Points Question Can handgun purchasing records, coupled with machine learning techniques, be used to forecast firearm suicide risk? Findings In this prognostic study of nearly 2 million individuals with handgun transaction records, among transactions classified in the riskiest 5%, close to 40% were associated with a purchaser who died by firearm suicide within 1 year. Among the small number of transactions with a random forest score of 0.95 and above, more than two-thirds were affiliated with a purchaser who died by firearm suicide within 1 year (24 of 35). Meaning This study suggests that passively collected administrative data on handgun transactions may be used to inform targeted interventions based on risk stratification.
SIMPLICIAL MATRICES AND THE NERVES OF WEAK n-CATEGORIES I : NERVES OF BICATEGORIES

To a bicategory B (in the sense of Benabou) we assign a simplicial set Ner(B), the (geometric) nerve of B, which completely encodes the structure of B as a bicategory. As a simplicial set Ner(B) is a subcomplex of its 2-Coskeleton and itself isomorphic to its 3-Coskeleton, what we call a 2-dimensional Postnikov complex. We then give, somewhat more delicately, a complete characterization of those simplicial sets which are the nerves of bicategories as certain 2-dimensional Postnikov complexes which satisfy certain restricted “exact horn-lifting” conditions whose satisfaction is controlled by (and here defines) subsets of (abstractly) invertible 2 and 1-simplices. Those complexes which have, at minimum, their degenerate 2-simplices always invertible and have an invertible 2-simplex χ2(x12, x01) present for each “composable pair” (x12, , x01) ∈ ∧1 2 are exactly the nerves of bicategories. At the other extreme, where all 2 and 1-simplices are invertible, are those Kan complexes in which the Kan conditions are satisfied exactly in all dimensions > 2. These are exactly the nerves of bigroupoids–all 2-cells are isomorphisms and all 1-cells are equivalences.
Early detection of rumors based on source tweet-word graph attention networks

The massively and rapidly spreading disinformation on social network platforms poses a serious threat to public safety and social governance. Therefore, early and accurate detection of rumors in social networks is of vital importance before they spread on a large scale. Considering the small-world property of social networks, the source tweet-word graph is decomposed from the global graph of rumors, and a rumor detection method based on graph attention network of source tweet-word graph is proposed to fully learn the structure of rumor propagation and the deep representation of text contents. Specifically, the proposed model can adequately capture the contextual semantic association representation of source tweets during the propagation and extract semantic features. For the data sparseness of the early stage of information dissemination, text attention mechanism based on opinion similarity can aggregate and capture more tweet propagation structure features to help improve the efficiency of early detection of rumors. Through the analysis of the experimental results on real public datasets, the rumor detection performance of the proposed method is better than that of other baseline methods. Especially in the early rumor detection tasks, the proposed method can detect rumors with an accuracy of nearly 90% in the early stage of information dissemination. And it still has good robustness with noise interference.
Examination of nailfold videocapillaroscopy findings in ANCA-associated vasculitis.

OBJECTIVES To evaluate nailfold videocapillaroscopy (NVC) as a useful tool for assessing the disease activity of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). METHODS This study enrolled 51 patients with AAV and 21 healthy controls. We scored NVC findings semi-quantitatively, and compared them between AAV patients and controls. We examined the association of NVC findings with disease activity indicators, histopathological findings of skin biopsies, and high-resolution computed tomography (HRCT) scores in AAV. Additionally, we repeatedly rated the NVC findings 3 months after immunosuppressive therapy. RESULTS 70.6% (36/51) showed a microangiopathy pattern and 7.8% (4/51) showed a scleroderma pattern in AAV. The scores for microhemorrhage, capillary loss, neoangiogenesis, and tortuosity were significantly higher in the AAV group than in the control group. NVC abnormalities correlated with the severity of skin, lung, and kidney involvement. The scores of giant capillaries significantly correlated with the total BVAS and the chest BVAS; the scores of capillary loss correlated with the chest BVAS and the renal BVAS. The scores of microhemorrhage significantly correlated with perivascular inflammatory cell infiltrations in the upper dermis of the purpura and tended to correlate with the total ground-glass opacity and consolidation scores on HRCT. In addition, capillary loss scores had a significant positive correlation with serum creatinine levels. Additionally, the microhemorrhage scores significantly reduced after 3 months of immunosuppressive therapy. CONCLUSION In AAV patients, NVC abnormalities are significantly associated with disease severity. This result suggests that NVC is a useful tool for assessing the disease activity and treatment response in AAV.
Radiation therapy enhances systemic antitumor efficacy in PD-L1 therapy regardless of sequence of radiation in murine osteosarcoma

Recent studies demonstrate that immune checkpoint blockade (ICB) increases the chances of the abscopal effect, an anti-tumor effect outside the radiation field in radiation therapy. However, the optimal sequence between radiation and ICB remains unclear. To investigate the impact of sequence of radiation in anti-PD-L1 antibody (P1) therapy on immune microenvironments and antitumor efficacies in local and abscopal tumors, metastatic LM8 osteosarcoma cells were inoculated into both legs of C3H mice. For irradiation, only one side leg was irradiated at 10 Gy. Then mice were divided into four groups: administrated anti-PD-L1 antibody three times (P1 monotherapy), receiving radiation 3 days prior to P1 therapy (P1+pre-Rad), and receiving concurrent radiation with P1 therapy (P1+conc-Rad). Thereafter, tumor immune microenvironment and tumor volume changes were analyzed in irradiated and unirradiated tumors. The P1+pre-Rad regimen increased the proportion of CD8+ programmed cell death 1 (PD-1)+ granzyme B (GzmB)+ reinvigorated T cells and decreased the proportion of CD8+ PD-1+ GzmB- exhausted T cells than P1+conc-Rad regimen in unirradiated tumors. Combination regimens suppressed tumor growth in irradiated tumors compared with that in P1 monotherapy. In both irradiated and unirradiated tumors, significant tumor growth suppression and prolonged overall survival were observed under both combination treatment regimens compared with P1 monotherapy. However, no distinct differences in unirradiated tumor volume and survival were observed between P1+pre-Rad and P1+conc-Rad groups. These results suggest that local irradiation is necessary to improve systemic treatment efficacy in P1 therapy regardless of sequence of local irradiation.
Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion

Purpose The aim of this study was to explore the roles of crystallins in the context of aging in glaucoma and potential mechanisms of neuroprotection in an experimental animal model of glaucoma. Methods Intraocular pressure (IOP) was significantly elevated for 8 weeks in animals at different ages (10 days, 12 weeks, and 44 weeks) by episcleral vein cauterization. Retinal ganglion cells (RGCs) were quantified by anti-Brn3a immunohistochemical staining (IHC). Proteomics using ESI-LTQ Orbitrap XL-MS was used to analyze the presence and abundance of crystallin isoforms the retinal samples, respectively. Neuroprotective property and localization of three selected crystallins CRYAB, CRYBB2, and CRYGB as most significantly changed in retina and retinal layers were determined by IHC. Their expressions and endocytic uptakes into Müller cells were analyzed by IHC and Western blotting. Müller cell secretion of neurotrophic factors into the supernatant following CRYAB, CRYBB2, and CRYGB supplementation in vitro was measured via microarray. Results IOP elevation resulted in significant RGC loss in all age groups (P < 0.001). The loss increased with aging. Proteomics analysis revealed in parallel a significant decrease of crystallin abundance – especially CRYAB, CRYBB2, and CRYGB. Significant neuroprotective effects of CRYAB, CRYBB2, and CRYGB after addition to retinal cultures were demonstrated (P < 0.001). Endocytic uptake of CRYAB, CRYBB2, and CRYGB was seen in Müller cells with subsequent increased secretion of various neurotrophic factors into the supernatant, including nerve growth factor, clusterin, and matrix metallopeptidase 9. Conclusions An age-dependent decrease in CRYAB, CRYBB2, and CRYGB abundance is found going along with increased RGC loss. Addition of CRYAB, CRYBB2, and CRYGB to culture protected RGCs in vitro. CRYAB, CRYBB2, and CRYGB were uptaken into Müller cells. Secretion of neurotrophic factors was increased as a potential mode of action.
Unity of Opposites between Soluble and Insoluble Lithium Polysulfides in Lithium–Sulfur Batteries

Rechargeable batteries based on Li–S chemistry show promise as being possible for next‐generation energy storage devices because of their ultrahigh capacities and energy densities. Research over the past decade has demonstrated that the morphology of lithium polysulfides (LPSs) in electrolytes (soluble or insoluble) plays a decisive role in battery performance. Early studies have focused mainly on inhibiting the dissolution of LPSs and invested considerable effort to realize this objective. However, in recent years, a completely different view that the dissolution of LPSs during battery discharge/charge should be promoted has emerged. At this critical juncture in the large‐scale application of Li–S batteries, it is time to summarize and discuss both sides of the contradiction. Herein, an overview of these two opposite views pertaining to soluble and insoluble LPSs, including their historical environment, classical strategies, advantages, and disadvantages. Finally, the future morphology of LPSs in Li–S batteries is predicted based on a multiangle review of research studies conducted thus far, and the reasoning behind this conjecture is thoroughly discussed.
Pseudoaneurysm of the superficial temporal artery after blunt trauma: case report and literature review

An 88-year-old man presented with a left temporal pulsatile mass that developed after blunt trauma. Based on suspicion of hematoma, needle aspiration was performed with the removal of approximately 15 mL of blood. No evident improvement was noted, and active arterial bleeding was observed at the needle puncture site. Doppler ultrasonography revealed a “yin-yang” sign, and the mass was diagnosed as a pseudoaneurysm of the left superficial temporal artery. Under general anesthesia, the superficial temporal artery was ligated and the pseudoaneurysm was removed. Superficial temporal artery pseudoaneurysm is a rare facial tumor that generally occurs after blunt trauma. Due to its rarity, pseudoaneurysms are often misdiagnosed as hematoma. The treatment of choice is excision, although endovascular intervention is a potential treatment option. However, when a pseudoaneurysm is small, conservative treatment can be used.
Dopamine activity in projection neurons regulates short‐lasting olfactory approach memory in Drosophila

Survival in many animals requires the ability to associate certain cues with danger and others with safety. In a Drosophila melanogaster aversive olfactory conditioning paradigm, flies are exposed to two odours, one presented coincidentally with electrical shocks, and a second presented 45 s after shock cessation. When flies are later given a choice between these two odours, they avoid the shock‐paired odour and prefer the unpaired odour. While many studies have examined how flies learn to avoid the shock‐paired odour through formation of odour‐fear associations, here we demonstrate that conditioning also causes flies to actively approach the second odour. In contrast to fear memories, which are longer lasting and requires activity of D1‐like dopamine receptors only in the mushroom bodies, approach memory is short‐lasting and requires activity of D1‐like dopamine receptors in projection neurons originating from the antennal lobes, primary olfactory centers. Further, while recall of fear memories requires activity of the mushroom bodies, recall of approach memories does not. Our data suggest that olfactory approach memory is formed using different mechanisms in different brain locations compared to aversive and appetitive olfactory memories.
The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells

Human embryonic stem cells (hESCs) derive from the epiblast and have pluripotent potential. To maintain the conventional conditions of the pluripotent potential in an undifferentiated state, inactivated mouse embryonic fibroblast (iMEF) is used as a feeder layer. However, it has been suggested that hESC under this conventional condition (hESC-iMEF) is an artifact that does not correspond to the in vitro counterpart of the human epiblast. Our previous studies demonstrated the use of an alternative feeder layer of human amniotic epithelial cells (hAECs) to derive and maintain hESC. We wondered if the hESC-hAEC culture could represent a different pluripotent stage than that of naïve or primed conventional conditions, simulating the stage in which the amniotic epithelium derives from the epiblast during peri-implantation. Like the conventional primed hESC-iMEF, hESC-hAEC has the same levels of expression as the ‘pluripotency core’ and does not express markers of naïve pluripotency. However, it presents a downregulation of HOX genes and genes associated with the endoderm and mesoderm, and it exhibits an increase in the expression of ectoderm lineage genes, specifically in the anterior neuroectoderm. Transcriptome analysis showed in hESC-hAEC an upregulated signature of genes coding for transcription factors involved in neural induction and forebrain development, and the ability to differentiate into a neural lineage was superior in comparison with conventional hESC-iMEF. We propose that the interaction of hESC with hAEC confers hESC a biased potential that resembles the anteriorized epiblast, which is predisposed to form the neural ectoderm.
The emerging role of LRRK2 in tauopathies

Abstract Parkinson’s disease (PD) is conventionally described as an α-synuclein aggregation disorder, defined by Lewy bodies and neurites, and mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common autosomal dominant cause of PD. However, LRRK2 mutations may be associated with diverse pathologies in patients with Parkinson’s syndrome including tau pathology resembling progressive supranuclear palsy (PSP). The recent discovery that variation at the LRRK2 locus is associated with the progression of PSP highlights the potential importance of LRRK2 in tauopathies. Here, we review the emerging evidence and discuss the potential impact of LRRK2 dysfunction on tau aggregation, lysosomal function, and endocytosis and exocytosis.
Environmental effects on the spread of the Neolithic crop package to South Asia

The emergence of Neolithic economies and their spread through Eurasia was one of the most crucial transitions of the Holocene, with different mechanisms of diffusion—demic, cultural—being proposed. While this phenomenon has been exhaustively studied in Europe, with repeated attempts to model the speed of Neolithic diffusion based on radiocarbon dates, much less attention has been devoted to the dispersal towards the East, and in particular to South Asia. The Neolithic in the latter region at least partly derived from southwest Asia, given the presence of “founder crops” such as wheat and barley. The process of their eastward diffusion, however, may have been significantly different to the westward dispersal, which was mainly due to demic diffusion, as local domesticates were already available and farming was already practiced in parts of South Asia. Here, we use radiocarbon dates specifically related to the spread of the southwest Asian Neolithic crops to model the speed of dispersal of this agricultural package towards South Asia. To assess potential geographical and environmental effects on the dispersal, we simulate different speeds depending on the biomes being crossed, employing a genetic algorithm to search for the values that most closely approach the radiocarbon dates. We find that the most important barrier to be crossed were the Zagros mountains, where the speed was lowest, possibly due to topography and climate. A large portion of the study area is dominated by deserts and shrublands, where the speed of advance, albeit closer to the range expected for demic diffusion, was lower than observed in Europe, which can also potentially be attributed to environmental constraints in the adaptation of the crops. Finally, a notable acceleration begins in the Indus valley, exceeding the range of demic diffusion in the tropical and subtropical environments east of the Indus. We propose that the latter is due to the rapid diffusion among populations already familiar with plant cultivation.