Draft Genome Sequence of the White-Rot Fungus <named-content content-type='genus-species'>Phanerochaete sordida</named-content> YK-624

Here, we report the draft genome sequence of the white-rot basidiomycete fungus Phanerochaete sordida YK-624 (ATCC 90872), which was isolated in Yakushima, Kagoshima, Japan. The genome of this strain was found to be 41.2 Mbp, with a G1C content of 58.7%, and to comprise 17,108 predicted protein-coding sequences. P hanerochaete sordida YK-624 is a white-rot basidiomycete fungus that exhibits superior ligninolytic properties (1). The fungus was isolated from decaying wood collected in Yakushima, Japan. A piece cut from the decaying wood xylem was placed on wood agar medium (0.2% beech wood meal, 0.02% guaiacol, and 1.6% agar [pH 5.5]). Grown hyphae were isolated after incubation at 30°C for 7 days. The fungus secretes manganese peroxidase as the primary ligninolytic enzyme during wood decay, in addition to lignin peroxidases (2). P. sordida YK-624 also degrades several recalcitrant organic pollutants, such as neonicotinoid insecticides (3–5), endocrine-disrupting compounds (6, 7), and polychlorinated dioxins (8). It has been suggested that cytochrome P450s (CYPs) are involved in many of these reactions (3–5, 7). In addition, a molecular breeding technique for the fungus has been established, and several transformants have been constructed (e.g., references 2, 9, and 10). Therefore, the fungus can be applied as a platform for the analysis of white-rot fungus-specific gene function and biotechnological applications. However, basic genetic information regarding P. sordida, such as genes encoding lignin-degradation-related enzymes and CYPs, remains unknown. To enhance understanding of the metabolic mechanism and to facilitate modification of the activities of this fungus, we sequenced the genome of P. sordida YK624. Phanerochaete sordida YK-624 (ATCC 90872) was pregrown on potato-dextrose agar (PDA) plates. Two mycelium-covered plugs (1.0-cm diameter) from the PDA plates were inoculated into 100-ml Erlenmeyer flasks containing 10 ml of potato-dextrose liquid medium. The cultures were incubated for 5 days at 30°C, and then mycelia were recovered. Genomic DNA was extracted using the Isoplant II kit (Nippon Gene Co., Ltd.), followed by ethanol precipitation for final purification. The DNA concentration was determined using a Quantus fluorometer (Promega, USA). DNA fragmentation was performed using an M220 focused ultrasonicator (Covaris Inc., USA), and libraries were prepared using a TruSeq DNA PCR-free library preparation kit (Illumina, USA). Whole-genome sequencing using a MiSeq system (Illumina) was carried out using 301-bp paired-end reads, resulting in a total of 36,391,668 paired-end reads (totaling 10,038 Mb). Raw sequence data were cleaned using Trimmomatic v. 0.38 by trimming the adaptor sequences and bases with quality scores of ,15 and filtering out reads longer than 150 bp (11). The resultant 22,452,994 high-quality reads, totaling 5,521 Mb, were assembled using SPAdes v. 3.13. 0 with a default k-mer set and the options careful, only-assembler, and cov-cutoff auto CitationMori T, Dohra H, Suzuki T, Kawagishi H, Hirai H. 2021. Draft genome sequence of the white-rot fungus Phanerochaete sordida YK624. Microbiol Resour Announc 10:e00842-21. https://doi.org/10.1128/MRA.00842-21. Editor Antonis Rokas, Vanderbilt University Copyright © 2021 Mori et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Hirofumi Hirai, hirai.hirofumi@shizuoka.ac. jp. Received 23 August 2021 Accepted 24 September 2021 Published 21 October 2021 Volume 10 Issue 42 e00842-21 mra.asm. org 1 GENOME SEQUENCES